Research Topics
Genomes and Genes
| Gunter MeisterSummaryCountry: Germany Publications
| Collaborators
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Detail Information
Publications
miRNAs get an early start on translational silencingGunter Meister
Munich Center for Integrated Protein Science CIPSM, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Cell 131:25-8. 2007..2007; Mathonnet et al., 2007; Thermann and Hentze, 2007; Wakiyama et al., 2007). These new findings indicate that miRNAs inhibit translation at early steps of initiation...- Identification of novel argonaute-associated proteinsGunter Meister
Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
Curr Biol 15:2149-55. 2005..The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage... - microRNA-guided posttranscriptional gene regulationPo Yu Chen
Laboratory of RNA Biology, Max-Planck-Institute for Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany
Biol Chem 386:1205-18. 2005.... - Molecular biology. RNA interference in the nucleusGunter Meister
Max Planck Institute of Biochemistry, Martinsried D-82152, Germany
Science 321:496-7. 2008 
A human snoRNA with microRNA-like functionsChristine Ender
Center for Integrated Protein Science Munich CIPSM, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Mol Cell 32:519-28. 2008..We further identify a number of human snoRNAs with microRNA (miRNA)-like processing signatures. We have, therefore, identified a class of small RNAs in human cells that originate from snoRNAs and can function like miRNAs...- Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavageYu Ren Yuan
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Mol Cell 19:405-19. 2005..We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC... - Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAsGunter Meister
Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
Mol Cell 15:185-97. 2004..The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based depletion of individual Ago members in combination with a sensitive positive-readout reporter assay... - Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAsLasse Weinmann
Laboratory of RNA Biology and Center for Integrated Protein Science Munich, Max Planck Institute of Biochemistry, Martinsried, Germany
Cell 136:496-507. 2009..Therefore, we provide evidence that Imp8 is required for cytoplasmic miRNA-guided gene silencing and affects nuclear localization of Ago proteins... 
Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cellsJulia Höck
Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
EMBO Rep 8:1052-60. 2007..Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation...- Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificityPo Yu Chen
Laboratory of RNA Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
RNA 14:263-74. 2008..We conclude that asymmetric 5' modification of siRNA duplexes can be useful for controlling targeting specificity... - A multifunctional human Argonaute2-specific monoclonal antibodySabine Rüdel
Center for Integrated Protein Science Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
RNA 14:1244-53. 2008..With the anti-Ago2(11A9) antibody we have generated a potent tool that is useful for many biochemical or cell biological applications... - Phosphorylation of human Argonaute proteins affects small RNA bindingSabine Rüdel
Center for Integrated Protein Science Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Nucleic Acids Res 39:2330-43. 2011..Our data suggest that a negatively charged phospho-tyrosine generates a repulsive force that prevents efficient binding of the negatively charged 5' phosphate of the small RNA... - Argonaute proteins: mediators of RNA silencingLasse Peters
Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Mol Cell 26:611-23. 2007..This review focuses on the various cellular functions of mammalian Argonaute proteins in conjunction with the different small RNA species that are known today... - Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68Jia Yun Zhu
Center for Integrated Protein Sciences Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
J Virol 84:10266-75. 2010..Our data will aid in the full exploration of the functions of gammaHV miRNAs... - Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi proteinJin Biao Ma
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Nature 434:666-70. 2005.... - The Argonaute protein familyJulia Höck
Center for Integrated Protein Science Munich CIPSM, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz, 82152 Martinsried, Germany
Genome Biol 9:210. 2008..Therefore, it is very likely that as yet unknown functions of the Argonaute protein family will be elucidated in the future... - Phosphorylation of Argonaute proteins: regulating gene regulatorsSabine Rüdel
Center for integrated protein Sciences Munich CIPSM, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Biochem J 413:e7-9. 2008..Zeng et al. further show that a member of the MAPK (mitogen-activated protein kinase) signalling pathway phosphorylates Ago2 at Ser(387), suggesting that Ago2-mediated gene silencing might be linked to distinct signalling pathways... - Experimental identification of microRNA targets by immunoprecipitation of Argonaute protein complexesMichaela Beitzinger
Laboratory for RNA Biology, Center for Integrated Protein Science Munich CIPSM, Max Planck Institute of Biochemistry, Martinsried, Germany
Methods Mol Biol 732:153-67. 2011..Here we provide a detailed protocol for isolation and identification of miRNA target mRNAs from immunoprecipitated human Ago protein complexes... - Identification of human microRNA targets from isolated argonaute protein complexesMichaela Beitzinger
Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
RNA Biol 4:76-84. 2007..Our data clearly indicate that miRNA targets can be experimentally identified from Ago complexes and therefore provide a new tool to directly analyze miRNA function... - The small RNA expression profile of the developing murine urinary and reproductive systemsAna Laura Gutierrez Aguilar
Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
FEBS Lett 584:4426-34. 2010..Our comprehensive miRNA expression atlas of the developing genitourinary system forms an invaluable basis for further functional in vivo studies... - Mechanisms of gene silencing by double-stranded RNAGunter Meister
Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
Nature 431:343-9. 2004..These short dsRNAs guide RNA silencing by specific and distinct mechanisms. Many components of the RNA silencing machinery still need to be identified and characterized, but a more complete understanding of the process is imminent... - Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencingGunter Meister
Laboratory of RNA Molecular Biology, The Rockefeller University, New York, New York 10021, USA
RNA 10:544-50. 2004..Here we show that 2'-O-methyl oligoribonucleotides, but not 2'-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA-protein complexes in human cell extracts as well as in cultured human cells... - Dicer-dependent and -independent Argonaute2 protein interaction networks in mammalian cellsAnne Frohn
Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Mol Cell Proteomics 11:1442-56. 2012..In contrast to our current view, we find that Ago-mRNA interactions can also take place in the absence of miRNAs. Our proteomics approach provides a rich resource for further functional studies on the cellular roles of Ago proteins... - Differential regulation of microRNAs by p53 revealed by massively parallel sequencing: miR-34a is a p53 target that induces apoptosis and G1-arrestValery Tarasov
Molecular Oncology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
Cell Cycle 6:1586-93. 2007..siRNAs corresponding to p53-induced miRNAs may have potential as cancer therapeutic agents as RNA interference based therapies are currently emerging... - In vitro RISC cleavage assayJulia Stoehr
Center for Integrated Protein Science Munich CIPSM, Laboratory for RNA Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
Methods Mol Biol 725:77-90. 2011..In vitro cleavage assays have proved to be powerful tools for the characterization of the RNAi pathway in many different organisms. Therefore, this chapter provides a detailed protocol for in vitro RISC assays... - Identification of novel Epstein-Barr virus microRNA genes from nasopharyngeal carcinomasJia Yun Zhu
Center for Integrated Protein Sciences Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
J Virol 83:3333-41. 2009..We found that the tumor suppressor BRCA-1 is a target of miR-15a as well as miR-16, suggesting a miRNA role in NPC pathogenesis... - Extensive terminal and asymmetric processing of small RNAs from rRNAs, snoRNAs, snRNAs, and tRNAsZhihua Li
School of Medicine, University of Pittsburgh, Pittsburgh, PA 15260, USA
Nucleic Acids Res 40:6787-99. 2012..We suggest that endogenous 3' tRFs have a role in regulating the unwarranted expression of endogenous viruses through the RNA interference pathway... - Ultrastructural characterisation of a nuclear domain highly enriched in survival of motor neuron (SMN) proteinManuela Malatesta
Centre of Electron Microscopy, University of Lausanne, 1005 Lausanne, Switzerland
Exp Cell Res 292:312-21. 2004..Our data reveal a novel SMN/Gemin2 containing nuclear domain and support the idea that it represents the structural counterpart of gems seen in the light microscope... - Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexesTeldja N Azzouz
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland
J Biol Chem 280:34435-40. 2005..Furthermore, we present evidence for two separate binding sites in SMN for Sm/Lsm proteins. One recognizes Sm domains and the second one, the sDMA-modified RG-tails, which are present only in a subset of these proteins... - SMN-mediated assembly of RNPs: a complex storyGunter Meister
Max-Planck Institute of Biochemistry, Am Klopferspitz 18a, 82152, Martinsried, Germany
Trends Cell Biol 12:472-8. 2002..These complexes might also facilitate the assembly of other cellular RNPs... - Assisted RNP assembly: SMN and PRMT5 complexes cooperate in the formation of spliceosomal UsnRNPsGunter Meister
Max-Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany
EMBO J 21:5853-63. 2002..These data uncover the SMN-PRMT5 complex as a functional entity that promotes the assisted assembly of spliceosomal UsnRNPs, and potentially other, RNA-protein complexes... - Epstein-Barr virus-encoded microRNA miR-BART2 down-regulates the viral DNA polymerase BALF5Stephanie Barth
Institute of Virology, University of Saarland Medical School, 66424 Homburg, Germany
Nucleic Acids Res 36:666-75. 2008..Our results are compatible with the notion that EBV-miR-BART2 inhibits transition from latent to lytic viral replication... - Epstein-Barr virus nuclear antigen 2 binds via its methylated arginine-glycine repeat to the survival motor neuron proteinStephanie Barth
Institut fur Medizinische Mikrobiologie und Hygiene, Abteilung Virologie, Universitatskliniken des Saarlandes, 66421 Homburg Saar, Germany
J Virol 77:5008-13. 2003.... - Phosphorylation regulates the activity of the SMN complex during assembly of spliceosomal U snRNPsMatthias Grimmler
Institute of Biochemistry, , Am Hubland, , Germany
EMBO Rep 6:70-6. 2005..As cytoplasmic SMN is phosphorylated to a much larger extent, we hypothesize that this modification is a key activator of the SMN complex... - Tdrd3 is a novel stress granule-associated protein interacting with the Fragile-X syndrome protein FMRPBastian Linder
Department of Biochemistry, Theodor Boveri Institute, Am Hubland, D 97074 Wurzburg, Germany
Hum Mol Genet 17:3236-46. 2008..We propose a contribution of Tdrd3 to FMRP-mediated translational repression and suggest that the loss of the FMRP-Tdrd3 interaction caused by the I304N mutation might contribute to the pathogenesis of Fragile X syndrome... - Reduced U snRNP assembly causes motor axon degeneration in an animal model for spinal muscular atrophyChristoph Winkler
Institute of Biochemistry and Institute of Physiological Chemistry, Biocenter of the University of Wurzburg, D 97074 Wurzburg, Germany
Genes Dev 19:2320-30. 2005..These findings suggest that motoneuron degeneration in SMA patients is a direct consequence of impaired production of U snRNPs... - The impact of small RNAs. Microsymposium on small RNAsGregor Obernosterer
Institute of Molecuar Biotechnology of the Austrian Academy of Sciences (IMBA, Dr. Bohr-Gasse 3, 1030 Vienna, Austria
EMBO Rep 8:23-7. 2007 - Gene targeting of Gemin2 in mice reveals a correlation between defects in the biogenesis of U snRNPs and motoneuron cell deathSibylle Jablonka
Institute of Clinical Neurobiology, Josef Schneider Strasse 11, D 97080 Wurzburg, Germany
Proc Natl Acad Sci U S A 99:10126-31. 2002..This finding correlates with enhanced motoneuron degeneration in Gemin2(+/-)/Smn(+/-) mice. Our data provide in vivo evidence that impaired production of U snRNPs contributes to motoneuron degeneration...