Gunter Meister

Summary

Country: Germany

Publications

  1. ncbi miRNAs get an early start on translational silencing
    Gunter Meister
    Munich Center for Integrated Protein Science CIPSM, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Cell 131:25-8. 2007
  2. ncbi Identification of novel argonaute-associated proteins
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Curr Biol 15:2149-55. 2005
  3. ncbi microRNA-guided posttranscriptional gene regulation
    Po Yu Chen
    Laboratory of RNA Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Biol Chem 386:1205-18. 2005
  4. doi Molecular biology. RNA interference in the nucleus
    Gunter Meister
    Max Planck Institute of Biochemistry, Martinsried D 82152, Germany
    Science 321:496-7. 2008
  5. ncbi Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage
    Yu Ren Yuan
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell 19:405-19. 2005
  6. doi A human snoRNA with microRNA-like functions
    Christine Ender
    Center for Integrated Protein Science Munich CIPSM, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 32:519-28. 2008
  7. doi Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs
    Lasse Weinmann
    Laboratory of RNA Biology and Center for Integrated Protein Science Munich, Max Planck Institute of Biochemistry, Martinsried, Germany
    Cell 136:496-507. 2009
  8. pmc Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells
    Julia Höck
    Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    EMBO Rep 8:1052-60. 2007
  9. ncbi Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Mol Cell 15:185-97. 2004
  10. pmc Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity
    Po Yu Chen
    Laboratory of RNA Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    RNA 14:263-74. 2008

Collaborators

Detail Information

Publications38

  1. ncbi miRNAs get an early start on translational silencing
    Gunter Meister
    Munich Center for Integrated Protein Science CIPSM, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Cell 131:25-8. 2007
    ..2007; Mathonnet et al., 2007; Thermann and Hentze, 2007; Wakiyama et al., 2007). These new findings indicate that miRNAs inhibit translation at early steps of initiation...
  2. ncbi Identification of novel argonaute-associated proteins
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Curr Biol 15:2149-55. 2005
    ..The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage...
  3. ncbi microRNA-guided posttranscriptional gene regulation
    Po Yu Chen
    Laboratory of RNA Biology, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Biol Chem 386:1205-18. 2005
    ....
  4. doi Molecular biology. RNA interference in the nucleus
    Gunter Meister
    Max Planck Institute of Biochemistry, Martinsried D 82152, Germany
    Science 321:496-7. 2008
  5. ncbi Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage
    Yu Ren Yuan
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell 19:405-19. 2005
    ..We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC...
  6. doi A human snoRNA with microRNA-like functions
    Christine Ender
    Center for Integrated Protein Science Munich CIPSM, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 32:519-28. 2008
    ..We further identify a number of human snoRNAs with microRNA (miRNA)-like processing signatures. We have, therefore, identified a class of small RNAs in human cells that originate from snoRNAs and can function like miRNAs...
  7. doi Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs
    Lasse Weinmann
    Laboratory of RNA Biology and Center for Integrated Protein Science Munich, Max Planck Institute of Biochemistry, Martinsried, Germany
    Cell 136:496-507. 2009
    ..Therefore, we provide evidence that Imp8 is required for cytoplasmic miRNA-guided gene silencing and affects nuclear localization of Ago proteins...
  8. pmc Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells
    Julia Höck
    Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    EMBO Rep 8:1052-60. 2007
    ..Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation...
  9. ncbi Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Mol Cell 15:185-97. 2004
    ..The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based depletion of individual Ago members in combination with a sensitive positive-readout reporter assay...
  10. pmc Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity
    Po Yu Chen
    Laboratory of RNA Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    RNA 14:263-74. 2008
    ..We conclude that asymmetric 5' modification of siRNA duplexes can be useful for controlling targeting specificity...
  11. pmc A multifunctional human Argonaute2-specific monoclonal antibody
    Sabine Rüdel
    Center for Integrated Protein Science Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
    RNA 14:1244-53. 2008
    ..With the anti-Ago2(11A9) antibody we have generated a potent tool that is useful for many biochemical or cell biological applications...
  12. pmc Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, New York, New York 10021, USA
    RNA 10:544-50. 2004
    ..Here we show that 2'-O-methyl oligoribonucleotides, but not 2'-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA-protein complexes in human cell extracts as well as in cultured human cells...
  13. pmc Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68
    Jia Yun Zhu
    Center for Integrated Protein Sciences Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    J Virol 84:10266-75. 2010
    ..Our data will aid in the full exploration of the functions of gammaHV miRNAs...
  14. pmc Phosphorylation of human Argonaute proteins affects small RNA binding
    Sabine Rüdel
    Center for Integrated Protein Science Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nucleic Acids Res 39:2330-43. 2011
    ..Our data suggest that a negatively charged phospho-tyrosine generates a repulsive force that prevents efficient binding of the negatively charged 5' phosphate of the small RNA...
  15. ncbi Argonaute proteins: mediators of RNA silencing
    Lasse Peters
    Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 26:611-23. 2007
    ..This review focuses on the various cellular functions of mammalian Argonaute proteins in conjunction with the different small RNA species that are known today...
  16. pmc Dicer-dependent and -independent Argonaute2 protein interaction networks in mammalian cells
    Anne Frohn
    Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell Proteomics 11:1442-56. 2012
    ..In contrast to our current view, we find that Ago-mRNA interactions can also take place in the absence of miRNAs. Our proteomics approach provides a rich resource for further functional studies on the cellular roles of Ago proteins...
  17. pmc The Argonaute protein family
    Julia Höck
    Center for Integrated Protein Science Munich CIPSM, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz, 82152 Martinsried, Germany
    Genome Biol 9:210. 2008
    ..Therefore, it is very likely that as yet unknown functions of the Argonaute protein family will be elucidated in the future...
  18. ncbi Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein
    Jin Biao Ma
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 434:666-70. 2005
    ....
  19. doi Phosphorylation of Argonaute proteins: regulating gene regulators
    Sabine Rüdel
    Center for integrated protein Sciences Munich CIPSM, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Biochem J 413:e7-9. 2008
    ..Zeng et al. further show that a member of the MAPK (mitogen-activated protein kinase) signalling pathway phosphorylates Ago2 at Ser(387), suggesting that Ago2-mediated gene silencing might be linked to distinct signalling pathways...
  20. doi Experimental identification of microRNA targets by immunoprecipitation of Argonaute protein complexes
    Michaela Beitzinger
    Laboratory for RNA Biology, Center for Integrated Protein Science Munich CIPSM, Max Planck Institute of Biochemistry, Martinsried, Germany
    Methods Mol Biol 732:153-67. 2011
    ..Here we provide a detailed protocol for isolation and identification of miRNA target mRNAs from immunoprecipitated human Ago protein complexes...
  21. ncbi SMN-mediated assembly of RNPs: a complex story
    Gunter Meister
    Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82152, Martinsried, Germany
    Trends Cell Biol 12:472-8. 2002
    ..These complexes might also facilitate the assembly of other cellular RNPs...
  22. ncbi Mechanisms of gene silencing by double-stranded RNA
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Nature 431:343-9. 2004
    ..These short dsRNAs guide RNA silencing by specific and distinct mechanisms. Many components of the RNA silencing machinery still need to be identified and characterized, but a more complete understanding of the process is imminent...
  23. doi The small RNA expression profile of the developing murine urinary and reproductive systems
    Ana Laura Gutierrez Aguilar
    Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    FEBS Lett 584:4426-34. 2010
    ..Our comprehensive miRNA expression atlas of the developing genitourinary system forms an invaluable basis for further functional in vivo studies...
  24. ncbi Identification of human microRNA targets from isolated argonaute protein complexes
    Michaela Beitzinger
    Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    RNA Biol 4:76-84. 2007
    ..Our data clearly indicate that miRNA targets can be experimentally identified from Ago complexes and therefore provide a new tool to directly analyze miRNA function...
  25. pmc Identification of novel Epstein-Barr virus microRNA genes from nasopharyngeal carcinomas
    Jia Yun Zhu
    Center for Integrated Protein Sciences Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Virol 83:3333-41. 2009
    ..We found that the tumor suppressor BRCA-1 is a target of miR-15a as well as miR-16, suggesting a miRNA role in NPC pathogenesis...
  26. doi In vitro RISC cleavage assay
    Julia Stoehr
    Center for Integrated Protein Science Munich CIPSM, Laboratory for RNA Biology, Max Planck Institute of Biochemistry, Martinsried, Germany
    Methods Mol Biol 725:77-90. 2011
    ..In vitro cleavage assays have proved to be powerful tools for the characterization of the RNAi pathway in many different organisms. Therefore, this chapter provides a detailed protocol for in vitro RISC assays...
  27. ncbi Differential regulation of microRNAs by p53 revealed by massively parallel sequencing: miR-34a is a p53 target that induces apoptosis and G1-arrest
    Valery Tarasov
    Molecular Oncology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell Cycle 6:1586-93. 2007
    ..siRNAs corresponding to p53-induced miRNAs may have potential as cancer therapeutic agents as RNA interference based therapies are currently emerging...
  28. pmc Assisted RNP assembly: SMN and PRMT5 complexes cooperate in the formation of spliceosomal UsnRNPs
    Gunter Meister
    Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 21:5853-63. 2002
    ..These data uncover the SMN-PRMT5 complex as a functional entity that promotes the assisted assembly of spliceosomal UsnRNPs, and potentially other, RNA-protein complexes...
  29. pmc Extensive terminal and asymmetric processing of small RNAs from rRNAs, snoRNAs, snRNAs, and tRNAs
    Zhihua Li
    School of Medicine, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Nucleic Acids Res 40:6787-99. 2012
    ..We suggest that endogenous 3' tRFs have a role in regulating the unwarranted expression of endogenous viruses through the RNA interference pathway...
  30. ncbi Ultrastructural characterisation of a nuclear domain highly enriched in survival of motor neuron (SMN) protein
    Manuela Malatesta
    Centre of Electron Microscopy, University of Lausanne, 1005 Lausanne, Switzerland
    Exp Cell Res 292:312-21. 2004
    ..Our data reveal a novel SMN/Gemin2 containing nuclear domain and support the idea that it represents the structural counterpart of gems seen in the light microscope...
  31. ncbi Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes
    Teldja N Azzouz
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland
    J Biol Chem 280:34435-40. 2005
    ..Furthermore, we present evidence for two separate binding sites in SMN for Sm/Lsm proteins. One recognizes Sm domains and the second one, the sDMA-modified RG-tails, which are present only in a subset of these proteins...
  32. pmc Epstein-Barr virus nuclear antigen 2 binds via its methylated arginine-glycine repeat to the survival motor neuron protein
    Stephanie Barth
    Institut fur Medizinische Mikrobiologie und Hygiene, Abteilung Virologie, Universitatskliniken des Saarlandes, 66421 Homburg Saar, Germany
    J Virol 77:5008-13. 2003
    ....
  33. doi Tdrd3 is a novel stress granule-associated protein interacting with the Fragile-X syndrome protein FMRP
    Bastian Linder
    Department of Biochemistry, Theodor Boveri Institute, Am Hubland, D 97074 Wurzburg, Germany
    Hum Mol Genet 17:3236-46. 2008
    ..We propose a contribution of Tdrd3 to FMRP-mediated translational repression and suggest that the loss of the FMRP-Tdrd3 interaction caused by the I304N mutation might contribute to the pathogenesis of Fragile X syndrome...
  34. pmc The impact of small RNAs. Microsymposium on small RNAs
    Gregor Obernosterer
    Institute of Molecuar Biotechnology of the Austrian Academy of Sciences IMBA, Dr Bohr Gasse 3, 1030 Vienna, Austria
    EMBO Rep 8:23-7. 2007
  35. pmc Phosphorylation regulates the activity of the SMN complex during assembly of spliceosomal U snRNPs
    Matthias Grimmler
    Institute of Biochemistry, Biocenter at the University of Würzburg, Am Hubland, 97074 Wurzburg, Germany
    EMBO Rep 6:70-6. 2005
    ..As cytoplasmic SMN is phosphorylated to a much larger extent, we hypothesize that this modification is a key activator of the SMN complex...
  36. pmc Epstein-Barr virus-encoded microRNA miR-BART2 down-regulates the viral DNA polymerase BALF5
    Stephanie Barth
    Institute of Virology, University of Saarland Medical School, 66424 Homburg, Germany
    Nucleic Acids Res 36:666-75. 2008
    ..Our results are compatible with the notion that EBV-miR-BART2 inhibits transition from latent to lytic viral replication...
  37. pmc Reduced U snRNP assembly causes motor axon degeneration in an animal model for spinal muscular atrophy
    Christoph Winkler
    Institute of Biochemistry and Institute of Physiological Chemistry, Biocenter of the University of Wurzburg, D 97074 Wurzburg, Germany
    Genes Dev 19:2320-30. 2005
    ..These findings suggest that motoneuron degeneration in SMA patients is a direct consequence of impaired production of U snRNPs...
  38. pmc Gene targeting of Gemin2 in mice reveals a correlation between defects in the biogenesis of U snRNPs and motoneuron cell death
    Sibylle Jablonka
    Institute of Clinical Neurobiology, Josef Schneider Strasse 11, D 97080 Wurzburg, Germany
    Proc Natl Acad Sci U S A 99:10126-31. 2002
    ..This finding correlates with enhanced motoneuron degeneration in Gemin2(+/-)/Smn(+/-) mice. Our data provide in vivo evidence that impaired production of U snRNPs contributes to motoneuron degeneration...