Kai Simons

Summary

Affiliation: Max Planck Institute of Molecular Cell Biology and Genetics
Country: Germany

Publications

  1. ncbi Rab10 is involved in basolateral transport in polarized Madin-Darby canine kidney cells
    Sebastian Schuck
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Traffic 8:47-60. 2007
  2. ncbi Lessons in science politics
    K Simons
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 110, 01307 Dresden, Germany
    Nat Rev Mol Cell Biol 2:931-3. 2001
  3. ncbi The European research council on the brink
    Kai Simons
    Max Planck Institute for Cell Biology and Genetics, Dresden 01307, Germany
    Cell 123:747-50. 2005
  4. pmc Membrane organization and lipid rafts
    Kai Simons
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Cold Spring Harb Perspect Biol 3:a004697. 2011
  5. ncbi Model systems, lipid rafts, and cell membranes
    Kai Simons
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Annu Rev Biophys Biomol Struct 33:269-95. 2004
  6. doi Revitalizing membrane rafts: new tools and insights
    Kai Simons
    Max Planck Institute of Cell Biology and Genetics, Dresden, Germany
    Nat Rev Mol Cell Biol 11:688-99. 2010
  7. pmc Cholesterol, lipid rafts, and disease
    Kai Simons
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    J Clin Invest 110:597-603. 2002
  8. pmc Caveolin-1 is not essential for biosynthetic apical membrane transport
    Aki Manninen
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Mol Cell Biol 25:10087-96. 2005
  9. doi Generic sorting of raft lipids into secretory vesicles in yeast
    Michal A Surma
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstr 108, Dresden 01307, Germany
    Traffic 12:1139-47. 2011
  10. pmc Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network
    Robin W Klemm
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 185:601-12. 2009

Collaborators

Detail Information

Publications79

  1. ncbi Rab10 is involved in basolateral transport in polarized Madin-Darby canine kidney cells
    Sebastian Schuck
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Traffic 8:47-60. 2007
    ..These results indicate that Rab10 functions in trafficking from the Golgi at early stages of epithelial polarization, is involved in biosynthetic transport to the basolateral membrane and may co-operate with Rab8...
  2. ncbi Lessons in science politics
    K Simons
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 110, 01307 Dresden, Germany
    Nat Rev Mol Cell Biol 2:931-3. 2001
    ..In Europe, we are now also faced with a similar problem and need to learn how to campaign for better research funding...
  3. ncbi The European research council on the brink
    Kai Simons
    Max Planck Institute for Cell Biology and Genetics, Dresden 01307, Germany
    Cell 123:747-50. 2005
    ..This new agency will sustain fundamental investigation into all aspects of scientific knowledge and should drive up standards of scientific research across the continent...
  4. pmc Membrane organization and lipid rafts
    Kai Simons
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Cold Spring Harb Perspect Biol 3:a004697. 2011
    ..Here we will review the emerging principles of membrane architecture with special emphasis on lipid organization and domain formation...
  5. ncbi Model systems, lipid rafts, and cell membranes
    Kai Simons
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Annu Rev Biophys Biomol Struct 33:269-95. 2004
    ..This review critically analyzes what is known of phase behavior and liquid-liquid immiscibility in model systems and compares these data with what is known of domain formation in cell membranes...
  6. doi Revitalizing membrane rafts: new tools and insights
    Kai Simons
    Max Planck Institute of Cell Biology and Genetics, Dresden, Germany
    Nat Rev Mol Cell Biol 11:688-99. 2010
    ....
  7. pmc Cholesterol, lipid rafts, and disease
    Kai Simons
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    J Clin Invest 110:597-603. 2002
  8. pmc Caveolin-1 is not essential for biosynthetic apical membrane transport
    Aki Manninen
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Mol Cell Biol 25:10087-96. 2005
    ..Similar results were obtained using embryonic fibroblasts from caveolin-1-knockout mice. Thus, we conclude that caveolin-1 does not play a major role in lipid raft-mediated biosynthetic membrane trafficking...
  9. doi Generic sorting of raft lipids into secretory vesicles in yeast
    Michal A Surma
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstr 108, Dresden 01307, Germany
    Traffic 12:1139-47. 2011
    ..This finding indicates that lipid raft sorting is a generic feature of vesicles carrying PM cargo and suggests a common lipid-based mechanism for their formation...
  10. pmc Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network
    Robin W Klemm
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 185:601-12. 2009
    ....
  11. pmc A lipid E-MAP identifies Ubx2 as a critical regulator of lipid saturation and lipid bilayer stress
    Michal A Surma
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Mol Cell 51:519-30. 2013
    ..Our results point toward the existence of dedicated bilayer stress responses for membrane homeostasis. ..
  12. ncbi Partitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes
    Erdinç Sezgin
    Biophysics BIOTEC, TU Dresden Tatzberg 47 51, 01307 Dresden, Germany
    Biochim Biophys Acta 1818:1777-84. 2012
    ....
  13. pmc Plasma membrane polarization during mating in yeast cells
    Tomasz J Proszynski
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 173:861-6. 2006
    ..Furthermore, we provide evidence that the lipid bilayer at the mating projection is more condensed than the plasma membrane enclosing the cell body, and that sphingolipids are required for this lipid organization...
  14. pmc Gp135/podocalyxin and NHERF-2 participate in the formation of a preapical domain during polarization of MDCK cells
    Doris Meder
    Max Planck Institute of Molecular Cell Biology and Genetics, D 01307 Dresden, Germany
    J Cell Biol 168:303-13. 2005
    ..Together, our data suggest that podocalyxin and NHERF-2 function in epithelial polarization by contributing to an early apical scaffold based on PDZ domain-mediated interactions...
  15. pmc Galectin-9 trafficking regulates apical-basal polarity in Madin-Darby canine kidney epithelial cells
    Rashmi Mishra
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 107:17633-8. 2010
    ..Together our results suggest a model wherein such glycosphingolipid-galectin couples form a circuit between the Golgi apparatus and the cell surface that in an epithelial context facilitates the apical sorting of proteins and lipids...
  16. pmc Order of lipid phases in model and plasma membranes
    Hermann Josef Kaiser
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 106:16645-50. 2009
    ..Thus, lipid-mediated coalescence of the GM1 raft domain seems to be distinct from the formation of a Lo phase, suggesting additional interactions between proteins and lipids to be effective...
  17. pmc Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry
    Christer S Ejsing
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 106:2136-41. 2009
    ..This work serves as a resource for molecular characterization of eukaryotic lipidomes, and establishes shotgun lipidomics as a powerful platform for complementing biochemical studies and other systems-level approaches...
  18. doi Lipid-dependent protein sorting at the trans-Golgi network
    Michal A Surma
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstr 108, 01307 Dresden, Germany
    Biochim Biophys Acta 1821:1059-67. 2012
    ..Here, we review the evidence for such lipid-raft-based sorting at the TGN, as well as their involvement in the formation of TGN-to-PM transport carriers. This article is part of a Special Issue entitled Lipids and Vesicular Transport...
  19. pmc Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane
    Mathias J Gerl
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 196:213-21. 2012
    ..Our data are consistent with and extend existing models of membrane raft-based biogenesis of the apical membrane and IFV envelope...
  20. doi Lipid rafts as functional heterogeneity in cell membranes
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Biochem Soc Trans 37:955-60. 2009
    ..Evaluation of this property suggests that functional raft heterogeneity arises through principles of lipid-driven phase segregation coupled to additional chemical specificities, probably involving proteins...
  21. pmc Yeast lipids can phase-separate into micrometer-scale membrane domains
    Christian Klose
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    J Biol Chem 285:30224-32. 2010
    ..Taken together, these results provide a mechanistic explanation for lipid raft-dependent lipid and protein sorting in yeast...
  22. pmc FAPP2 is involved in the transport of apical cargo in polarized MDCK cells
    Otilia V Vieira
    Max Planck Institute for Molecular Cell Biology and Genetics, 01307, Dresden, Germany
    J Cell Biol 170:521-6. 2005
    ..On the other hand, FAPP2 was not involved in the Golgi-to-PM transport of cargo that was targeted to the basolateral membrane domain. Thus, we conclude that FAPP2 plays a specific role in apical transport in MDCK cells...
  23. pmc Alzheimer's disease beta-amyloid peptides are released in association with exosomes
    Lawrence Rajendran
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 103:11172-7. 2006
    ..Exosomal proteins were found to accumulate in the plaques of AD patient brains, suggesting a role in the pathogenesis of AD...
  24. pmc O-glycosylation as a sorting determinant for cell surface delivery in yeast
    Tomasz J Proszynski
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Mol Biol Cell 15:1533-43. 2004
    ..Exocytosis of this protein could be restored by addition of a 33-amino acid portion of an O-glycosylated sequence from Fus1p. Our data suggest that O-glycosylation functions as a sorting determinant for cell surface delivery of Fus1p...
  25. doi Elucidating membrane structure and protein behavior using giant plasma membrane vesicles
    Erdinç Sezgin
    Biophysics BIOTEC, Technische Universitat Dresden, Dresden, Germany
    Nat Protoc 7:1042-51. 2012
    ..GPMV preparation and isolation, including fluorescent labeling and observation, can be accomplished within 4 h...
  26. doi Efficient inhibition of the Alzheimer's disease beta-secretase by membrane targeting
    Lawrence Rajendran
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr 108, 01307 Dresden, Germany
    Science 320:520-3. 2008
    ..In addition to effectively targeting beta-secretase, this strategy could also be used in designing potent drugs against other membrane protein targets...
  27. pmc Phase coexistence and connectivity in the apical membrane of polarized epithelial cells
    Doris Meder
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 103:329-34. 2006
    ....
  28. pmc Identification of glycosylated marker proteins of epithelial polarity in MDCK cells by homology driven proteomics
    Joachim Fullekrug
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    BMC Biochem 7:8. 2006
    ..Monoclonal antibodies against apical gp114 and basolateral p58 have served as important tools in these studies. However, the molecular identity of these membrane glycoproteins has not been known...
  29. pmc Functional convergence of hopanoids and sterols in membrane ordering
    James Peter Sáenz
    Max Plank Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 109:14236-40. 2012
    ..These observations suggest that the evolution of an ordered biochemically active liquid membrane could have evolved before the oxygenation of Earth's surface and the emergence of sterols...
  30. ncbi Polarized sorting in epithelial cells: raft clustering and the biogenesis of the apical membrane
    Sebastian Schuck
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    J Cell Sci 117:5955-64. 2004
    ..Besides functioning in polarized sorting in differentiated cells, raft clustering might also play an important role in the biogenesis of apical membrane domains during development...
  31. pmc Generation of cubic membranes by controlled homotypic interaction of membrane proteins in the endoplasmic reticulum
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Biol Chem 284:12041-8. 2009
    ..Autophagy may be targeting irregular membrane shapes and/or aggregated protein. We suggest that membrane morphology can be controlled in cells...
  32. ncbi Increased Abeta production leads to intracellular accumulation of Abeta in flotillin-1-positive endosomes
    Lawrence Rajendran
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Neurodegener Dis 4:164-70. 2007
    ..We also demonstrate that flotillin-1 is not only associated with intracellular Abeta in transgenic mice but also with extracellular beta-amyloid plaques in AD patient brain sections...
  33. pmc Plasma membranes are poised for activation of raft phase coalescence at physiological temperature
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Technical University of Dresden, Dresden, Germany
    Proc Natl Acad Sci U S A 105:10005-10. 2008
    ..Under this light, our induction of raft-phase separation suggests that plasma membrane composition is poised for selective and functional raft clustering at physiologically relevant temperature...
  34. pmc Controversy fuels trafficking of GPI-anchored proteins
    Sebastian Schuck
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 172:963-5. 2006
    ..p. 1035) show that the original view nevertheless holds. This closes a chapter in the winding story of GPI-AP trafficking but opens another phase, as the controversy has stimulated the development of new methodology...
  35. pmc A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast
    Tomasz J Proszynski
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Proc Natl Acad Sci U S A 102:17981-6. 2005
    ..This visual screening method can now be used for different cargo proteins to search in a genome-wide fashion for machinery involved in post-Golgi sorting...
  36. pmc Polarized sorting and trafficking in epithelial cells
    Xinwang Cao
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Cell Res 22:793-805. 2012
    ..Here we review the recent advances in the field of polarized sorting in epithelial cells. We especially highlight the role of lipid rafts in apical sorting...
  37. pmc Molecular convergence of bacterial and eukaryotic surface order
    Hermann Josef Kaiser
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden 01307, Germany
    J Biol Chem 286:40631-7. 2011
    ..We found that transmembrane segments of proteins have an important role in buffering lipid-mediated packing. This buffering ensures that sterol-free and sterol-containing membranes exhibit similar barrier properties...
  38. pmc Membrane lipidome of an epithelial cell line
    Julio L Sampaio
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 108:1903-7. 2011
    ..Moreover, during polarization, the sphingolipids became longer, more saturated, and more hydroxylated as required to generate an apical membrane domain that serves as a protective barrier for the epithelial sheet...
  39. pmc Oligomerization and pore formation by equinatoxin II inhibit endocytosis and lead to plasma membrane reorganization
    Ana J García-Sáez
    BIOTEC der TU Dresden, 01307 Dresden, Germany
    J Biol Chem 286:37768-77. 2011
    ..We propose that plasma membrane reorganization into stabilized raft domains is part of the killing strategy of equinatoxin II...
  40. pmc Flexibility of a eukaryotic lipidome--insights from yeast lipidomics
    Christian Klose
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    PLoS ONE 7:e35063. 2012
    ..In addition, we established a baseline for future lipidomic experiments in yeast. Finally, flexibility of lipidomic features is proposed as a new parameter for the description of the physiological state of an organism...
  41. pmc Resistance of cell membranes to different detergents
    Sebastian Schuck
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 100:5795-800. 2003
    ....
  42. doi Membrane rafting: from apical sorting to phase segregation
    Unal Coskun
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    FEBS Lett 584:1685-93. 2010
    ..These are composed of fluctuating nanoscale assemblies of sphingolipid, cholesterol and proteins that can be stabilized to coalesce, forming platforms that function in membrane signalling and trafficking...
  43. pmc Golgi protein FAPP2 tubulates membranes
    Xinwang Cao
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Proc Natl Acad Sci U S A 106:21121-5. 2009
    ..This activity is dependent on the phosphoinositide-binding activity of the PH domain of FAPP2. These data suggest that FAPP2 functions directly in the formation of apical carriers in the trans-Golgi network...
  44. pmc Palmitoylation regulates raft affinity for the majority of integral raft proteins
    Ilya Levental
    Max Planck Institute of Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 107:22050-4. 2010
    ..Because cysteine palmitoylation is the only lipid modification that has been shown to be reversibly regulated, our data suggest a role for palmitoylation as a dynamic raft targeting mechanism for transmembrane proteins...
  45. ncbi Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry
    Christer S Ejsing
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Mass Spectrom 41:372-89. 2006
    ....
  46. ncbi Cholesterol-induced caveolin targeting to lipid droplets in adipocytes: a role for caveolar endocytosis
    Soazig Le Lay
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Traffic 7:549-61. 2006
    ..The present data identify lipid droplets as potential target organelles for caveolar endocytosis and demonstrate a role for caveolin-1 in the maintenance of free cholesterol levels in adipocyte lipid droplets...
  47. ncbi Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning
    Christer S Ejsing
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Anal Chem 78:6202-14. 2006
    ..The automated analysis of total lipid extracts was powered by a robotic nanoflow ion source and produced currently the most detailed description of the glycerophospholipidome...
  48. pmc Raft domains of variable properties and compositions in plasma membrane vesicles
    Ilya Levental
    Max Planck Institute of Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 108:11411-6. 2011
    ..These results show that, based on the principle of preferential association of raft lipids, domains of various properties can be produced in a membrane environment whose complexity is reflective of biological membranes...
  49. doi Lipid rafts as a membrane-organizing principle
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Science 327:46-50. 2010
    ..Here we review the evidence for how this principle combines the potential for sphingolipid-cholesterol self-assembly with protein specificity to selectively focus membrane bioactivity...
  50. pmc Lateral sorting in model membranes by cholesterol-mediated hydrophobic matching
    Hermann Josef Kaiser
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstr 108, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 108:16628-33. 2011
    ..These constraints translate into a sorting potential and lead to selective lateral segregation of peptides and lipids according to their hydrophobic length...
  51. ncbi Lipid rafts and membrane dynamics
    Lawrence Rajendran
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstr 108, 01307, Dresden, Germany
    J Cell Sci 118:1099-102. 2005
  52. doi Organellar lipidomics--background and perspectives
    Christian Klose
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Curr Opin Cell Biol 25:406-13. 2013
    ..Here we argue that only mass spectrometry-based organellar lipidomics can provide this information today. ..
  53. ncbi Lipid rafts in protein sorting and cell polarity in budding yeast Saccharomyces cerevisiae
    Michel Bagnat
    Max Planck Institute of Molecular Cell Biology and Genetics, D 01307 Dresden, Germany
    Biol Chem 383:1475-80. 2002
    ..Here we discuss some recent findings that stress the role of lipid rafts as key players in yeast protein sorting and cell polarity...
  54. pmc Regulation of human EGF receptor by lipids
    Unal Coskun
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Proc Natl Acad Sci U S A 108:9044-8. 2011
    ....
  55. doi Cholesterol modulates glycolipid conformation and receptor activity
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Nat Chem Biol 7:260-2. 2011
    ..This property appears to organize erythrocyte blood group presentation and glycolipid receptor function during the activation of sperm fertility, suggesting that lipid 'allostery' is a means to regulate membrane recognition processes...
  56. doi Depletion of apical transport proteins perturbs epithelial cyst formation and ciliogenesis
    Juha M Torkko
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    J Cell Sci 121:1193-203. 2008
    ..Although the phenotypes demonstrate the robustness of the formation of the polarized membrane domains, they indicate the important role of apical membrane biogenesis in epithelial organization...
  57. pmc The lipidomes of vesicular stomatitis virus, semliki forest virus, and the host plasma membrane analyzed by quantitative shotgun mass spectrometry
    Lucie Kalvodova
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    J Virol 83:7996-8003. 2009
    ....
  58. ncbi Lipid rafts and apical membrane traffic
    Joachim Fullekrug
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Ann N Y Acad Sci 1014:164-9. 2004
    ..Currently, we are evaluating candidate proteins by affinity chromatography, proteomics, and RNA interference...
  59. ncbi The effect of raft lipid depletion on microvilli formation in MDCK cells, visualized by atomic force microscopy
    Kate Poole
    Max Planck Institute for Molecular and Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    FEBS Lett 565:53-8. 2004
    ..Significantly, smaller spikes were observed on the surface of the smoother cells...
  60. ncbi Cell biology. Ras on the roundabout
    Doris Meder
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Science 307:1731-3. 2005
  61. doi Greasing their way: lipid modifications determine protein association with membrane rafts
    Ilya Levental
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden, Germany
    Biochemistry 49:6305-16. 2010
    ....
  62. pmc Amyloidogenic processing of the Alzheimer beta-amyloid precursor protein depends on lipid rafts
    Robert Ehehalt
    Max Planck Institute of Molecular Cell Biology and Genetics, D 01307 Dresden, Germany
    J Cell Biol 160:113-23. 2003
    ..Thus, access of alpha- and beta-secretase to APP, and therefore A beta generation, may be determined by dynamic interactions of APP with lipid rafts...
  63. ncbi Contributions of galectin-3 and -9 to epithelial cell adhesion analyzed by single cell force spectroscopy
    Jens Friedrichs
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Biol Chem 282:29375-83. 2007
    ..These defects in adhesion of the two galectin knockdown cell lines may underlie the epithelial phenotypes observed in the cyst assays. Our findings emphasize the complex regulation of epithelial cell functions by galectins...
  64. pmc Generation of single and double knockdowns in polarized epithelial cells by retrovirus-mediated RNA interference
    Sebastian Schuck
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 101:4912-7. 2004
    ..Thus, retrovirus-mediated RNAi is a convenient method for gene silencing in Madin-Darby canine kidney cells, and is likely to be applicable to virtually any mammalian cell...
  65. ncbi Lipids as modulators of proteolytic activity of BACE: involvement of cholesterol, glycosphingolipids, and anionic phospholipids in vitro
    Lucie Kalvodova
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden, Germany
    J Biol Chem 280:36815-23. 2005
    ..We have identified 3 groups of lipids that stimulate proteolytic activity of BACE: 1) neutral glycosphingolipids (cerebrosides), 2) anionic glycerophospholipids, and 3) sterols (cholesterol)...
  66. doi Lipidomics: coming to grips with lipid diversity
    Andrej Shevchenko
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Nat Rev Mol Cell Biol 11:593-8. 2010
    ..Here, we argue that lipidomics will become an essential tool kit in cell and developmental biology, molecular medicine and nutrition...
  67. ncbi Morphological homeostasis by autophagy
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Autophagy 5:1039-40. 2009
    ....
  68. ncbi Detergent resistance as a tool in membrane research
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Nat Protoc 2:2159-65. 2007
    ..These protocols are completed in 1-2 d...
  69. ncbi Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation
    Kim Ekroos
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    J Lipid Res 44:2181-92. 2003
    ....
  70. pmc Cell surface polarization during yeast mating
    Michel Bagnat
    Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 99:14183-8. 2002
    ..Our results show that membrane microdomain clustering at the mating projection is involved in the generation and maintenance of polarity during mating...
  71. ncbi Involvement of caveolin-2 in caveolar biogenesis in MDCK cells
    Ulla Lahtinen
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    FEBS Lett 538:85-8. 2003
    ..Correspondingly, in MDCK cells over-expressing caveolin-2 the basolateral membrane exhibited an increased number of caveolae. These results indicate the involvement of caveolin-2 in caveolar biogenesis...
  72. ncbi Quantitative profiling of phospholipids by multiple precursor ion scanning on a hybrid quadrupole time-of-flight mass spectrometer
    Kim Ekroos
    Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Anal Chem 74:941-9. 2002
    ..Taken together, a combination of multiple precursor ion scans allowed quantitative monitoring of major perturbation in phospholipid composition and elucidating of molecular heterogeneity of individual lipid species...
  73. pmc FAPP2, cilium formation, and compartmentalization of the apical membrane in polarized Madin-Darby canine kidney (MDCK) cells
    Otilia V Vieira
    Max Planck Institute for Molecular Cell Biology and Genetics, D 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 103:18556-61. 2006
    ..These results indicate that the compartmentalization of the apical membrane in MDCK cells into the ciliary membrane and the surrounding membrane depends on the balance of raft and nonraft domains...
  74. pmc Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells
    Delphine Delacour
    Unité INSERM 560, 59045 Lille Cedex, France
    J Cell Biol 169:491-501. 2005
    ..Together, our data propose that interaction between galectin-4 and sulfatides plays a functional role in the clustering of lipid rafts for apical delivery...
  75. ncbi A pipeline for Europe
    Tony Hyman
    Nature 449:256. 2007
  76. doi The misused impact factor
    Kai Simons
    Science 322:165. 2008
  77. ncbi Outline for a European research council
    Carol Featherstone
    Nature 425:451. 2003
  78. doi Caveolin-1 deficiency alters plasma lipid and lipoprotein profiles in mice
    Susanne Heimerl
    Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz Josef Strauss Allee 11, 93042 Regensburg, Germany
    Biochem Biophys Res Commun 367:826-33. 2008
    ..Our results demonstrate a significant role of Cav-1 in HDL-cholesterol metabolism and may reflect a variety of Cav-1 functions including modulation of ACAT activity and SR-BI function...
  79. ncbi The multiple faces of caveolae
    Robert G Parton
    Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Queensland 4072, Australia
    Nat Rev Mol Cell Biol 8:185-94. 2007
    ....