Research Topics
Species | Matthias MeyerSummaryAffiliation: Max Planck Institute for Evolutionary Anthropology Country: Germany Publications
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Detail Information
Publications
Illumina sequencing library preparation for highly multiplexed target capture and sequencingMatthias Meyer
Max Planck Institute for Evolutionary Anthropology, D 04103 Leipzig, Germany
Cold Spring Harb Protoc 2010:pdb.prot5448. 2010..Libraries can be prepared from any type of double-stranded DNA, even if present in subnanogram quantity...
A genomic region evolving toward different GC contents in humans and chimpanzees indicates a recent and regionally limited shift in the mutation patternIngo Ebersberger
Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany
Mol Biol Evol 22:1240-5. 2005..Based on our analysis, we suggest a recent and species-specific shift in the region's mutation pattern as the cause of its differing evolution in humans and chimpanzees...
Targeted high-throughput sequencing of tagged nucleic acid samplesMatthias Meyer
Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D 04103 Leipzig, Germany
Nucleic Acids Res 35:e97. 2007..PTS dramatically increases the sequencing throughput of samples in parallel and thus fully mobilizes the resources of the 454 technology for targeted sequencing...
Parallel tagged sequencing on the 454 platformMatthias Meyer
Max Planck Institute for Evolutionary Anthropology, Department of Evolutionary Genetics, Deutscher Platz 6, D 04103 Leipzig, Germany
Nat Protoc 3:267-78. 2008..Most of the reactions can be performed in a multichannel setup on 96-well reaction plates, allowing for processing up to several hundreds of samples in a few days...
A high-coverage genome sequence from an archaic Denisovan individualMatthias Meyer
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, D 04103 Leipzig, Germany
Science 338:222-6. 2012....
A phylogenetic estimate for golden moles (Mammalia, Afrotheria, Chrysochloridae)Robert J Asher
Department of Zoology, University of Cambridge, Cambridge, UK
BMC Evol Biol 10:69. 2010..Here, we use a combined analysis of partial sequences of the nuclear GHR gene and morphological characters to derive a phylogeny of species in the family Chrysochloridae...
From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencingMatthias Meyer
Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D 04103 Leipzig, Germany
Nucleic Acids Res 36:e5. 2008..The method should also apply to Illumina/Solexa and ABI/SOLiD sequencing, and should therefore help to widen the accessibility of all three platforms...
A draft sequence of the Neandertal genomeRichard E Green
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, D 04103 Leipzig, Germany
Science 328:710-22. 2010....
Targeted investigation of the Neandertal genome by array-based sequence captureHernán A Burbano
Max Planck Institute for Evolutionary Anthropology, D 04103 Leipzig, Germany
Science 328:723-5. 2010..By generating the sequence of one Neandertal and 50 present-day humans at these positions, we have identified 88 amino acid substitutions that have become fixed in humans since our divergence from the Neandertals...
Road blocks on paleogenomes--polymerase extension profiling reveals the frequency of blocking lesions in ancient DNAPatricia Heyn
Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D 04103 Leipzig, Germany
Nucleic Acids Res 38:e161. 2010....
Removal of deaminated cytosines and detection of in vivo methylation in ancient DNAAdrian W Briggs
Max Planck Institute for Evolutionary Anthropology, D 04103 Leipzig, Germany
Nucleic Acids Res 38:e87. 2010..In addition, our results demonstrate that Neandertal DNA retains in vivo patterns of CpG methylation, potentially allowing future studies of gene inactivation and imprinting in ancient organisms...
A complete Neandertal mitochondrial genome sequence determined by high-throughput sequencingRichard E Green
Max Planck Institute for Evolutionary Anthropology, D 04103 Leipzig, Germany
Cell 134:416-26. 2008..There is evidence that purifying selection in the Neandertal mtDNA was reduced compared with other primate lineages, suggesting that the effective population size of Neandertals was small...
Patterns of damage in genomic DNA sequences from a NeandertalAdrian W Briggs
Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D 04103 Leipzig, Germany
Proc Natl Acad Sci U S A 104:14616-21. 2007..The results suggest that reliable genome sequences can be obtained from Pleistocene organisms...
Direct multiplex sequencing (DMPS)--a novel method for targeted high-throughput sequencing of ancient and highly degraded DNAMathias Stiller
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany
Genome Res 19:1843-8. 2009..Embedded in our approach, we present a new protocol for the construction of barcoded sequencing libraries, which is compatible with all current high-throughput technologies and can be performed entirely in plate setup...
A recent evolutionary change affects a regulatory element in the human FOXP2 geneTomislav Maricic
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany
Mol Biol Evol 30:844-52. 2013....
Double indexing overcomes inaccuracies in multiplex sequencing on the Illumina platformMartin Kircher
Max Planck Institute for Evolutionary Anthropology, Department of Evolutionary Genetics, 04103 Leipzig, Germany
Nucleic Acids Res 40:e3. 2012..Double-indexing eliminates these problems and increases both the scope and accuracy of multiplex sequencing on the Illumina platform...
Single-stranded DNA library preparation for the sequencing of ancient or damaged DNAMarie Theres Gansauge
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany
Nat Protoc 8:737-48. 2013..We estimate that intact DNA strands are recovered in the library with ∼50% efficiency. Libraries can be generated from up to 12 DNA or oligonucleotide samples in parallel within 2 d...
Length and GC-biases during sequencing library amplification: a comparison of various polymerase-buffer systems with ancient and modern DNA sequencing librariesJesse Dabney
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany
Biotechniques 52:87-94. 2012....
Multiplex amplification of ancient DNAHolger Römpler
Molecular Biochemistry, Institute of Biochemistry, Medical Faculty, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany
Nat Protoc 1:720-8. 2006..It thus combines optimal template usage with a high specificity and can be performed within a day...
