Daniel Martins-de-Souza

Summary

Affiliation: Max Planck Institute of Psychiatry
Country: Germany

Publications

  1. pmc Proteomics as a tool for understanding schizophrenia
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Munich, Germany and Lab de Neurociências LIM 27, Inst Psiquiatria, Fac de Medicina da Universidade de São Paulo, Sao Paulo, Brazil
    Clin Psychopharmacol Neurosci 9:95-101. 2011
  2. pmc Identification of proteomic signatures associated with depression and psychotic depression in post-mortem brains from major depression patients
    D Martins-de-Souza
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    Transl Psychiatry 2:e87. 2012
  3. ncbi Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia
    Daniel Martins-de-Souza
    Laboratorio de Neurociencias, Instituto de Psiquiatria, Faculdade de Medicina da USP, Rua Dr Ovidio Pires de Campos, SP, Brazil
    J Psychiatr Res 43:978-86. 2009
  4. doi Proteome analyses of cultured astrocytes treated with MK-801 and clozapine: similarities with schizophrenia
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Kraepelinstr 2, 80804, Munich, Germany
    Eur Arch Psychiatry Clin Neurosci 261:217-28. 2011
  5. doi Different apolipoprotein E, apolipoprotein A1 and prostaglandin-H2 D-isomerase levels in cerebrospinal fluid of schizophrenia patients and healthy controls
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Munich, Germany
    World J Biol Psychiatry 11:719-28. 2010
  6. doi Proteome and transcriptome analysis suggests oligodendrocyte dysfunction in schizophrenia
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Kraepelinstrasse 2 10, D 80804 Munich, Germany
    J Psychiatr Res 44:149-56. 2010
  7. pmc Proteome analysis of schizophrenia patients Wernicke's area reveals an energy metabolism dysregulation
    Daniel Martins-de-Souza
    Laboratorio de Neurociencias, Instituto de Psiquiatria, Faculdade de Medicina da USP, Rua Dr, Ovídio Pires de Campos, no 785, Sao Paulo, SP, CEP 05403 010, Brazil
    BMC Psychiatry 9:17. 2009
  8. doi Shotgun mass spectrometry analysis of the human thalamus proteome
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Munich, Germany
    J Sep Sci 32:1231-6. 2009
  9. doi Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia
    Daniel Martins-de-Souza
    Laboratorio de Neurociencias, Instituto de Psiquiatria, Universidade de Sao Paulo, Rua Dr Ovidio Pires de Campos, no 785, Consolação, Sao Paulo, SP 05403 010, Brazil
    Eur Arch Psychiatry Clin Neurosci 259:151-63. 2009
  10. doi Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Kraepelinstr 2, 80804 Munich, Germany
    J Psychiatr Res 44:1176-89. 2010

Collaborators

Detail Information

Publications53

  1. pmc Proteomics as a tool for understanding schizophrenia
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Munich, Germany and Lab de Neurociências LIM 27, Inst Psiquiatria, Fac de Medicina da Universidade de São Paulo, Sao Paulo, Brazil
    Clin Psychopharmacol Neurosci 9:95-101. 2011
    ..These findings may encourage new studies on the understanding of schizophrenia biochemical pathways and even new potential drug targets...
  2. pmc Identification of proteomic signatures associated with depression and psychotic depression in post-mortem brains from major depression patients
    D Martins-de-Souza
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    Transl Psychiatry 2:e87. 2012
    ..These findings suggest that it may be possible to contribute to the disease understanding by distinguishing different subtypes of MDD based on distinct brain proteomic profiles...
  3. ncbi Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia
    Daniel Martins-de-Souza
    Laboratorio de Neurociencias, Instituto de Psiquiatria, Faculdade de Medicina da USP, Rua Dr Ovidio Pires de Campos, SP, Brazil
    J Psychiatr Res 43:978-86. 2009
    ..Our data not only reinforces the involvement of proteins previously implicated in schizophrenia, but also suggests new markers, providing further information to foster the comprehension of this important disease...
  4. doi Proteome analyses of cultured astrocytes treated with MK-801 and clozapine: similarities with schizophrenia
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Kraepelinstr 2, 80804, Munich, Germany
    Eur Arch Psychiatry Clin Neurosci 261:217-28. 2011
    ..Interestingly, clozapine reversed protein changes induced by MK-801. The treatment of cell cultures with neural transmission agonists and antagonists might provide useful insights about psychiatric disorders...
  5. doi Different apolipoprotein E, apolipoprotein A1 and prostaglandin-H2 D-isomerase levels in cerebrospinal fluid of schizophrenia patients and healthy controls
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Munich, Germany
    World J Biol Psychiatry 11:719-28. 2010
    ..To identify proteins differentially expressed in schizophrenia patients, we collected 50 microl cerebrospinal fluid from 17 first-episode schizophrenia patients and 10 healthy controls...
  6. doi Proteome and transcriptome analysis suggests oligodendrocyte dysfunction in schizophrenia
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Kraepelinstrasse 2 10, D 80804 Munich, Germany
    J Psychiatr Res 44:149-56. 2010
    ..The presented data contribute to the composition of a scenario that may lead to a better understanding of schizophrenia pathogenesis...
  7. pmc Proteome analysis of schizophrenia patients Wernicke's area reveals an energy metabolism dysregulation
    Daniel Martins-de-Souza
    Laboratorio de Neurociencias, Instituto de Psiquiatria, Faculdade de Medicina da USP, Rua Dr, Ovídio Pires de Campos, no 785, Sao Paulo, SP, CEP 05403 010, Brazil
    BMC Psychiatry 9:17. 2009
    ..The superior temporal gyrus is implicated in schizophrenia and executes functions such as the processing of speech, language skills and sound processing...
  8. doi Shotgun mass spectrometry analysis of the human thalamus proteome
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Munich, Germany
    J Sep Sci 32:1231-6. 2009
    ..The thalamus proteome contributes to the knowledge of the human brain proteome and future applications in basic and clinical research...
  9. doi Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia
    Daniel Martins-de-Souza
    Laboratorio de Neurociencias, Instituto de Psiquiatria, Universidade de Sao Paulo, Rua Dr Ovidio Pires de Campos, no 785, Consolação, Sao Paulo, SP 05403 010, Brazil
    Eur Arch Psychiatry Clin Neurosci 259:151-63. 2009
    ..In addition a number of new potential markers were found that may contribute to the understanding of the pathogenesis of this complex disease...
  10. doi Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Kraepelinstr 2, 80804 Munich, Germany
    J Psychiatr Res 44:1176-89. 2010
    ....
  11. doi Proteome analysis of schizophrenia brain tissue
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Munich, Germany
    World J Biol Psychiatry 11:110-20. 2010
    ..Reviewing the results of 13 proteome studies in SCZ brain tissue, we aimed to provide information regarding potential proteins biomarkers as well as information about the pathophysiology of the disease...
  12. ncbi Alterations in oligodendrocyte proteins, calcium homeostasis and new potential markers in schizophrenia anterior temporal lobe are revealed by shotgun proteome analysis
    Daniel Martins-de-Souza
    Laboratorio de Neurociencias, Faculdade de Medicina da USP, Instituto de Psiquiatria, Universidade de Sao Paulo, Rua Dr Ovidio Pires de Campos, no 785, s n Consolação, Sao Paulo, SP, CEP 05403 010, Brazil
    J Neural Transm 116:275-89. 2009
    ....
  13. doi Protein phosphorylation patterns in serum from schizophrenia patients and healthy controls
    Julian A J Jaros
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, United Kingdom
    J Proteomics 76:43-55. 2012
    ..This article is part of a Special Issue entitled: Integrated omics...
  14. doi Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration
    Daniel Martins-de-Souza
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    J Proteome Res 11:2533-43. 2012
    ..Further studies of aFTLD-U and other FTLD subtypes are warranted, although this will require intensive biobanking efforts...
  15. doi Proteome analysis of human dorsolateral prefrontal cortex using shotgun mass spectrometry
    Daniel Martins-de-Souza
    Laboratorio de Neurociencias, Instituto de Psiquiatria, Facultdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
    J Sep Sci 31:3122-6. 2008
    ..It will also aid comparative studies of different brain areas and medical conditions, with future applications in basic and clinical research...
  16. doi Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia
    Daniel Martins-de-Souza
    Max Planck Institute of Psychiatry, Proteomics and Biomarkers, Kraepelinstr 2, 80804 Munich, Germany
    J Psychiatr Res 44:989-91. 2010
    ..Our findings not only support previous findings but also indicate areas that warrant further study in schizophrenia...
  17. doi Identification of protein biomarkers in human serum using iTRAQ and shotgun mass spectrometry
    Theodoros A Koutroukides
    Department of Chemical Engineering and Biotechnology, Cambridge Centre for Neuropsychiatric Research, University of Cambridge, Cambridge, UK
    Methods Mol Biol 1061:291-307. 2013
    ..Here, we describe a complete protocol using iTRAQ in a shotgun proteomics workflow along with data analysis steps, customized for the challenges associated with the serum proteome. ..
  18. doi Proteome profiling of peripheral mononuclear cells from human blood
    Giuseppina Maccarrone
    Max Planck Institute for Psychiatry, Proteomics and Biomarkers, Munich, Germany
    Proteomics 13:893-7. 2013
    ..Mapping the MNC proteome provides a valuable resource for studies on disease pathogenesis and the identification of therapeutic targets...
  19. doi Analysis of the rat hypothalamus proteome by data-independent label-free LC-MS/MS
    Viktoria Stelzhammer
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    Proteomics 12:3386-92. 2012
    ..Important proteins involved in neurological and synaptic function were identified including several members of the Ras-related protein family and proteins involved in glutamate biosynthesis...
  20. doi To label or not to label: applications of quantitative proteomics in neuroscience research
    Michaela D Filiou
    Max Planck Institute of Psychiatry, Proteomics and Biomarkers, Munich, Germany
    Proteomics 12:736-47. 2012
    ....
  21. doi Characterizing the proteome of the human dorsolateral prefrontal cortex by shotgun mass spectrometry
    Daniel Martins-de-Souza
    Institute of Biotechnology, University of Cambridge, Cambridge, UK
    Proteomics 11:2347-53. 2011
    ..Combination of this data set with that obtained by our previous characterization of the same brain region results in a total of 755 unique proteins, making this the most comprehensive analysis of this important brain region to date...
  22. doi Differential expression of HINT1 in schizophrenia brain tissue
    Jeeva Varadarajulu
    Max Planck Institute of Psychiatry, Kraepelinstr 2, 80804, Munich, Germany
    Eur Arch Psychiatry Clin Neurosci 262:167-72. 2012
    ..Our results combined to previous studies in human samples and preclinical models support the notion that HINT1 must be more explored as a potential target for psychiatric disorders...
  23. doi Structural and pharmacological characterization of the crotamine isoforms III-4 (MYX4_CROCu) and III-7 (MYX7_CROCu) isolated from the Crotalus durissus cumanensis venom
    Luis Alberto Ponce-Soto
    Department of Biochemistry, Institute of Biology IB, State University of Campinas UNICAMP, P O Box 6109, 13083 970 Campinas, SP, Brazil
    Toxicon 55:1443-52. 2010
    ..Edema-forming activity was also analyzed by injection of the crotamine isoforms into the right paw, since both crotamine isoforms exert a strong pro-inflammatory effect...
  24. pmc Phosphoproteomic differences in major depressive disorder postmortem brains indicate effects on synaptic function
    Daniel Martins-de-Souza
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, Cambridgeshire, UK
    Eur Arch Psychiatry Clin Neurosci 262:657-66. 2012
    ..The majority of these phosphorylated proteins were associated with synaptic transmission and cellular architecture not only pointing out potential biomarker candidates but mainly shedding light to the comprehension of MDD pathobiology...
  25. pmc The application of selective reaction monitoring confirms dysregulation of glycolysis in a preclinical model of schizophrenia
    Daniel Martins-de-Souza
    Dept of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
    BMC Res Notes 5:146. 2012
    ..Selective reaction monitoring (SRM) has already shown promise in preclinical and clinical studies for multiplex measurement of diagnostic, prognostic and treatment-related biomarkers...
  26. doi Affinity depletion of plasma and serum for mass spectrometry-based proteome analysis
    Julian A J Jaros
    University of Cambridge, Cambridge, UK
    Methods Mol Biol 1002:1-11. 2013
    ..We describe in detail an affinity depletion method that selectively removes 14 of the most abundant proteins in plasma and serum...
  27. doi Clinical use of phosphorylated proteins in blood serum analysed by immobilised metal ion affinity chromatography and mass spectrometry
    Julian A J Jaros
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, United Kingdom
    J Proteomics 76:36-42. 2012
    ..This article is part of a Special Issue entitled: Integrated omics...
  28. doi Identification of a blood-based biological signature in subjects with psychiatric disorders prior to clinical manifestation
    Emanuel Schwarz
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    World J Biol Psychiatry 13:627-32. 2012
    ..To determine whether a molecular signature is present in blood of patients with psychiatric disorders before manifestation of symptoms...
  29. doi Abnormalities in metabolism and hypothalamic-pituitary-adrenal axis function in schizophrenia
    Paul C Guest
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    Int Rev Neurobiol 101:145-68. 2011
    ....
  30. doi Proteomic approaches to unravel the complexity of schizophrenia
    Daniel Martins-de-Souza
    Department of Chemical Engineering and Biotechnology, Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK
    Expert Rev Proteomics 9:97-108. 2012
    ..Here, we discuss the main outcome of these investigations and suggest a practical means of integrating and translating the findings between the brain and peripheral blood to increase our understanding of schizophrenia pathophysiology...
  31. doi The role of energy metabolism dysfunction and oxidative stress in schizophrenia revealed by proteomics
    Daniel Martins-de-Souza
    Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, Cambridgeshire, United Kingdom
    Antioxid Redox Signal 15:2067-79. 2011
    ..Here we review the results of these studies with a focus on the biomarker pattern depicting alterations in energy metabolism and oxidative stress in this debilitating illness...
  32. pmc Shotgun mass spectrometry workflow combining IEF and LC-MALDI-TOF/TOF
    Giuseppina Maccarrone
    Max Planck Institute of Psychiatry, Proteomics and Biomarkers, Kraepelinstrasse 2, 80804, Munich, Germany
    Protein J 29:99-102. 2010
    ..Such workflow was successfully applied to a comparative proteome analysis of schizophrenia versus healthy control brain tissues and can be an alternative to proteome researches...
  33. doi Quantitative proteomics for investigating psychiatric disorders
    Michaela D Filiou
    Proteomics and Biomarkers, Max Planck Institute of Psychiatry, Munich, Germany
    Proteomics Clin Appl 5:38-49. 2011
    ..Finally, we explore the applicability of implementing proteomic methods as a routine diagnostic tool in the clinical laboratory...
  34. doi Biochemical and pharmacological characterization of PhTX-I a new myotoxic phospholipase A2 isolated from Porthidium hyoprora snake venom
    Salomón Huancahuire-Vega
    Department of Biochemistry, Institute of Biology, State University of Campinas, SP, Brazil
    Comp Biochem Physiol C Toxicol Pharmacol 154:108-19. 2011
    ..In vitro, both induced low cytotoxicity in skeletal muscle myoblasts, however PhTX-I PLA(2) was able to lyse myotubes...
  35. doi Proteomic technologies for biomarker studies in psychiatry: advances and needs
    Daniel Martins-de-Souza
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom
    Int Rev Neurobiol 101:65-94. 2011
    ..The future development of such technologies may put proteomics closer to clinical applications in psychiatry...
  36. doi Characterization of the human primary visual cortex and cerebellum proteomes using shotgun mass spectrometry-data-independent analyses
    Daniel Martins-de-Souza
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    Proteomics 12:500-4. 2012
    ..The complete mapping of these and other brain proteomes may help in the elucidation of neurological processes and identify potential targets for therapeutic strategies...
  37. doi Functional and structural characterization of a new serine protease with thrombin-like activity TLBan from Bothrops andianus (Andean Lancehead) snake venom
    José Antonio Valeriano-Zapana
    Department of Biochemistry, Institute of Biology IB, State University of Campinas UNICAMP, P O Box 6109, Zip Code 13083 970, Campinas, SP, Brazil
    Toxicon 59:231-40. 2012
    ..TLBan showed a potential medical-scientific interest to understand the pathophysiological mechanism of the snake venom action and identification of new blood coagulation cascade acting enzymes of natural sources...
  38. doi Biological and biochemical characterization of two new PLA2 isoforms Cdc-9 and Cdc-10 from Crotalus durissus cumanensis snake venom
    Frey Francisco Romero-Vargas
    Department of Biochemistry, Institute Biology CP 6109, State University of Campinas, Campinas, SP, Brazil
    Comp Biochem Physiol C Toxicol Pharmacol 151:66-74. 2010
    ..However, p-BPB strongly diminishes the local and systemic myotoxic effects...
  39. ncbi Ten years of proteomics in multiple sclerosis
    Alessandro S Farias
    Neuroimmunomodulation Group, Department of Genetics, Evolution and Bioagents, University of Campinas UNICAMP Campinas, Sao Paulo, Brazil Neuroimmunology Unit, Department of Genetics, Evolution and Bioagents, University of Campinas UNICAMP Campinas, Sao Paulo, Brazil
    Proteomics 14:467-80. 2014
    ..In this review, we summarize these data, presenting their value to the current knowledge of the disease mechanisms, as well as their importance in identifying biomarkers or treatment targets. ..
  40. doi Characterization of the human serum depletome by label-free shotgun proteomics
    Theodoros A Koutroukides
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    J Sep Sci 34:1621-6. 2011
    ..These results suggest that further studies on this commonly ignored serum fraction may provide new insights into clinical proteomics...
  41. doi Proteomic changes induced by anaesthesia and muscle relaxant treatment prior to electroconvulsive therapy
    Viktoria Stelzhammer
    Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK
    Proteomics Clin Appl 5:644-9. 2011
    ..g. musculoskeletal injury, during the convulsions. However, most molecular studies investigating the mechanism of action of ECT have not explored the potential effects of the pre-treatment with anaesthetic and/ or muscle relaxant...
  42. doi Purification and inflammatory edema induced by two PLA2 (Anch TX-I and Anch TX-II) from sea anemone Anthothoe chilensis (Actiniaria: Sagartiidae)
    Elen Cristina Teizem Landucci
    Departamento de Farmacologia, Faculdade de Ciencias Medicas, CEP 13083 970, Campinas, SP, Brazil
    Comp Biochem Physiol B Biochem Mol Biol 161:170-7. 2012
    ..We also investigated the in vivo edematogenic activity of Anch TX-I and Anch TX-II in a model of paw and skin edema in rats and observed that both are able to induce dose-dependent edema...
  43. pmc The role of proteomics in depression research
    Daniel Martins-de-Souza
    Max Planck Institute for Psychiatry, Kraepelinstr 2, 80804, Munich, Germany
    Eur Arch Psychiatry Clin Neurosci 260:499-506. 2010
    ..Here, we will show the potential of proteome analyses as a tool to elucidate the pathophysiological mechanisms of depression as well as the discovery of potential diagnostic, therapeutic and disease course biomarkers...
  44. ncbi Proteome analysis of the plant pathogen Xylella fastidiosa reveals major cellular and extracellular proteins and a peculiar codon bias distribution
    Marcus Bustamante Smolka
    Departamento de Bioquimica, UNICAMP, Campinas, SP, Brazil
    Proteomics 3:224-37. 2003
    ..fastidiosa. A database of the X. fastidiosa proteome was developed and can be accessed via the internet (URL: www.proteome.ibi.unicamp.br)...
  45. doi Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3
    Carolina L Bigarella
    Hematology and Hemotherapy Center, University of Campinas, Campinas, Sao Paulo, Brazil
    FEBS Lett 586:3522-8. 2012
    ..Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function...
  46. doi Proteome analysis of spinal cord during the clinical course of monophasic experimental autoimmune encephalomyelitis
    Alessandro S Farias
    Neuroimmunology Unit, Department of Genetics Evolution and Bioagents, University of Campinas, SP, Brazil
    Proteomics 12:2656-62. 2012
    ....
  47. doi Increased stress reactivity is associated with reduced hippocampal activity and neuronal integrity along with changes in energy metabolism
    Alana Knapman
    Research Group of Psychoneuroendocrinology, Max Planck Institute of Psychiatry, Munich, Germany
    Eur J Neurosci 35:412-22. 2012
    ..Thus, our results provide further support for the involvement of a dysregulated HPA axis and mitochondrial dysfunction in the etiology and pathophysiology of affective disorders...
  48. ncbi Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL)
    Giuseppina Maccarrone
    Proteomics and Biomarkers, Max Planck Institute of Psychiatry, Kraepelinstr 2, Munich, 80804, Germany
    Methods Mol Biol 1156:175-85. 2014
    ..This protocol is suitable to studying any proteome of interest, only requiring a specific sample preparation and protein identification. Given our expertise, descriptions here are centered on the study of brain disorders. ..
  49. ncbi Characterization of the C-terminal half of human juvenile myoclonic epilepsy protein EFHC1: dimer formation blocks Ca2+ and Mg2+ binding to its functional EF-hand
    Marcelo J Murai
    Department of Medical Genetics, Faculty of Medical Sciences, Tessalia Vieira de Camargo, 126, CEP 13083 970, University of Campinas, UNICAMP, Campinas, SP, Brazil
    Arch Biochem Biophys 477:131-8. 2008
    ..It was also found that under reducing condition Ca(2+) or Mg(2+) ions bind to EFHC1C in a 1/1 molar ratio, while under oxidizing condition this ratio is reduced, showing that EFHC1C dimerization blocks Ca(2+) and Mg(2+) binding...
  50. ncbi The use of ASB-14 in combination with CHAPS is the best for solubilization of human brain proteins for two-dimensional gel electrophoresis
    Daniel Martins-de-Souza
    Departamento de Bioquimica, Instituto de Biologia, Universidade Estadual de Campinas UNICAMP, 13083 970 Campinas, SP, Brazil
    Brief Funct Genomic Proteomic 6:70-5. 2007
    ..Our results confirm that the most efficient solubilizing solution for 2-DE analysis of cytosolic and membrane Human Brain Proteins is SB combined with 4% CHAPS and 2% ASB-14...
  51. ncbi The overexpression of a single oncogene (ERBB2/HER2) alters the proteomic landscape of extracellular vesicles
    Maria Amorim
    Laboratory of Medical Genomics, AC Camargo Cancer Center, Sao Paulo, SP, Brazil Pós graduação em Oncologia, Fundacao Antonio Prudente, Sao Paulo, SP, Brazil
    Proteomics 14:1472-9. 2014
    ..Our results also suggest that HER2-upregulated proteins from EVs may be relevant for cellular malignancy and can be potential biomarkers for HER2(+) cancer patients. ..
  52. ncbi Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pera
    Rúbia P Purcino
    Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, 13083 970, Campinas, SP, Brazil
    J Exp Bot 58:2733-44. 2007
    ..These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant...