Yvonne Genzel

Summary

Affiliation: Max Planck Institute for Dynamics of Complex Technical Systems
Country: Germany

Publications

  1. ncbi High cell density cultivations by alternating tangential flow (ATF) perfusion for influenza A virus production using suspension cells
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg Sandtorstr 1, 39106 Magdeburg, Germany Electronic address
    Vaccine 32:2770-81. 2014
  2. doi CAP, a new human suspension cell line for influenza virus production
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Sandtorstr 1, 39106, Magdeburg, Germany
    Appl Microbiol Biotechnol 97:111-22. 2013
  3. ncbi Metabolism of MDCK cells during cell growth and influenza virus production in large-scale microcarrier culture
    Y Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Sandtorstr 1, 39106 Magdeburg, Germany
    Vaccine 22:2202-8. 2004
  4. ncbi Amino acid analysis in mammalian cell culture media containing serum and high glucose concentrations by anion exchange chromatography and integrated pulsed amperometric detection
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Sandtorstr 1, 39106 Magdeburg, Germany
    Anal Biochem 335:119-25. 2004
  5. ncbi Substitution of glutamine by pyruvate to reduce ammonia formation and growth inhibition of mammalian cells
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Sandtorstr 1, 39106 Magdeburg, Germany
    Biotechnol Prog 21:58-69. 2005
  6. ncbi Serum-free influenza virus production avoiding washing steps and medium exchange in large-scale microcarrier culture
    Y Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg Sandtorstrasse 1, 39106 Magdeburg, Germany
    Vaccine 24:3261-72. 2006
  7. doi MDCK and Vero cells for influenza virus vaccine production: a one-to-one comparison up to lab-scale bioreactor cultivation
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany
    Appl Microbiol Biotechnol 88:461-75. 2010
  8. ncbi Continuous cell lines as a production system for influenza vaccines
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering Magdeburg, Sandtorstr 1, Magdeburg 39106, Germany
    Expert Rev Vaccines 8:1681-92. 2009
  9. pmc Metabolic effects of influenza virus infection in cultured animal cells: Intra- and extracellular metabolite profiling
    Joachim B Ritter
    Delft University of Technology, Bioprocess Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
    BMC Syst Biol 4:61. 2010
  10. doi Virus-host cell interactions in vaccine production cell lines infected with different human influenza A virus variants: a proteomic approach
    Diana Vester
    Bioprocess Engineering, Otto von Guericke University Magdeburg, Magdeburg, Germany
    J Proteomics 73:1656-69. 2010

Collaborators

Detail Information

Publications30

  1. ncbi High cell density cultivations by alternating tangential flow (ATF) perfusion for influenza A virus production using suspension cells
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg Sandtorstr 1, 39106 Magdeburg, Germany Electronic address
    Vaccine 32:2770-81. 2014
    ..To our knowledge, this is the first study that illustrates the potential of ATF-based perfusion of chemically defined media across cell-retaining membranes for production of an influenza A vaccine. ..
  2. doi CAP, a new human suspension cell line for influenza virus production
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Sandtorstr 1, 39106, Magdeburg, Germany
    Appl Microbiol Biotechnol 97:111-22. 2013
    ....
  3. ncbi Metabolism of MDCK cells during cell growth and influenza virus production in large-scale microcarrier culture
    Y Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Sandtorstr 1, 39106 Magdeburg, Germany
    Vaccine 22:2202-8. 2004
    ..Here, glutamate and aspartate increase correlated to virus release kinetics, indicating cell disruption and apoptosis. Starting with a moi of 0.025 resulted in a maximum virus yield of 2.4 log HA/100 microl at 44 h post-infection...
  4. ncbi Amino acid analysis in mammalian cell culture media containing serum and high glucose concentrations by anion exchange chromatography and integrated pulsed amperometric detection
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Sandtorstr 1, 39106 Magdeburg, Germany
    Anal Biochem 335:119-25. 2004
    ..6 microM and quantitation limits of less than 1.6 microM. As an example, data for the amino acid composition of different media used for the production of inactivated influenza vaccines in cell culture are shown...
  5. ncbi Substitution of glutamine by pyruvate to reduce ammonia formation and growth inhibition of mammalian cells
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Sandtorstr 1, 39106 Magdeburg, Germany
    Biotechnol Prog 21:58-69. 2005
    ..After virus infection completely different profiles were found for essential and nonessential amino acids...
  6. ncbi Serum-free influenza virus production avoiding washing steps and medium exchange in large-scale microcarrier culture
    Y Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg Sandtorstrasse 1, 39106 Magdeburg, Germany
    Vaccine 24:3261-72. 2006
    ..Omission of the medium exchange before infection clearly simplified the process and reduced sterility risks due to washing steps...
  7. doi MDCK and Vero cells for influenza virus vaccine production: a one-to-one comparison up to lab-scale bioreactor cultivation
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany
    Appl Microbiol Biotechnol 88:461-75. 2010
    ..However, using these cultivation conditions in lab-scale bioreactors (stirred tank, wave bioreactor) resulted in lower productivities for Vero cells...
  8. ncbi Continuous cell lines as a production system for influenza vaccines
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering Magdeburg, Sandtorstr 1, Magdeburg 39106, Germany
    Expert Rev Vaccines 8:1681-92. 2009
    ..These encouraging developments for cell culture-based influenza virus production are summarized and an overview on potential cell substrates is given...
  9. pmc Metabolic effects of influenza virus infection in cultured animal cells: Intra- and extracellular metabolite profiling
    Joachim B Ritter
    Delft University of Technology, Bioprocess Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
    BMC Syst Biol 4:61. 2010
    ..A detailed metabolic characterization of an influenza infected adherent cell line (MDCK) was carried out based on extracellular and intracellular measurements of metabolite concentrations...
  10. doi Virus-host cell interactions in vaccine production cell lines infected with different human influenza A virus variants: a proteomic approach
    Diana Vester
    Bioprocess Engineering, Otto von Guericke University Magdeburg, Magdeburg, Germany
    J Proteomics 73:1656-69. 2010
    ..The findings will improve understanding of host cell response mechanisms during influenza vaccine production and viral strategies to evade these responses and to replicate efficiently in different cell lines...
  11. pmc Impact of host cell line adaptation on quasispecies composition and glycosylation of influenza A virus hemagglutinin
    Jana Verena Roedig
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany
    PLoS ONE 6:e27989. 2011
    ..In Vero cells a selection for "rescue" variants was characteristic. After back-adaptation to MDCK cells some variants persisted at indifferent frequencies, others slowly diminished and even dropped below the detection limit...
  12. ncbi Simultaneous extraction of several metabolites of energy metabolism and related substances in mammalian cells: optimization using experimental design
    Joachim B Ritter
    Max Planck Institute for Dynamics of Complex Technical Systems, 39106 Magdeburg, Germany
    Anal Biochem 373:349-69. 2008
    ..Absolute values of intracellular metabolites extracted with this modified method are comparable to the results of the two previously optimized methods, indicating a good extraction procedure according to the chosen response variables...
  13. pmc Continuous influenza virus production in cell culture shows a periodic accumulation of defective interfering particles
    Timo Frensing
    Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany
    PLoS ONE 8:e72288. 2013
    ..Yet, the continuous replication of influenza virus using a two-stage bioreactor setup is a novel tool to study aspects of viral evolution and the impact of DIPs. ..
  14. doi Impact of cultivation conditions on N-glycosylation of influenza virus a hemagglutinin produced in MDCK cell culture
    Jana Verena Rödig
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany
    Biotechnol Bioeng 110:1691-703. 2013
    ..Only the adaptation of the production host cell line to serum-free suspension growth significantly influenced HA N-glycosylation regarding both, the type of attached glycan structures as well as their abundances...
  15. pmc Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells
    Verena Lohr
    Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr 1, 39106 Magdeburg, Germany
    BMC Biotechnol 12:79. 2012
    ..In the near future influenza vaccines for human use will also be available from adherent MDCK or Vero cell cultures, but a scalable suspension process may facilitate production and supply with vaccines...
  16. doi Metabolic flux model for an anchorage-dependent MDCK cell line: characteristic growth phases and minimum substrate consumption flux distribution
    Aljoscha Wahl
    Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr 1, 39106 Magdeburg, Germany
    Biotechnol Bioeng 101:135-52. 2008
    ..Comparing this minimum substrate consumption flux distribution to the fluxes estimated from experiments unveiled high overflow metabolism under the applied process conditions...
  17. ncbi Harvesting and concentration of human influenza A virus produced in serum-free mammalian cell culture for the production of vaccines
    Bernd Kalbfuss
    Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr 1, Magdeburg 39106, Germany
    Biotechnol Bioeng 97:73-85. 2007
    ..From the results it can be concluded that the suggested process scheme is well suited for the harvesting and concentration of cell culture-derived influenza A virus...
  18. doi Proteome analysis of virus-host cell interaction: rabies virus replication in Vero cells in two different media
    Sabine Kluge
    Otto von Guericke University, Bioprocess Engineering, Universitatsplatz 2, 39106 Magdeburg, Germany
    Appl Microbiol Biotechnol 97:5493-506. 2013
    ....
  19. doi Comparison of metabolic flux distributions for MDCK cell growth in glutamine- and pyruvate-containing media
    Yury Sidorenko
    Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, Magdeburg, Germany
    Biotechnol Prog 24:311-20. 2008
    ..Thus, the switch from glutamine-containing to glutamine-free medium with pyruvate provided a series of benefits without dramatic changes of cellular metabolism...
  20. doi Growth behavior of number distributed adherent MDCK cells for optimization in microcarrier cultures
    Andreas Bock
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Sandtorstrasse 1, 39106 Magdeburg, Germany
    Biotechnol Prog 25:1717-31. 2009
    ....
  21. doi Capture of cell culture-derived influenza virus by lectins: strain independent, but host cell dependent
    Lars Opitz
    Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany
    J Virol Methods 154:61-8. 2008
    ..Hence, altered lectin specificities have been observed between the two cell lines, requiring process adaptations between different influenza vaccine production systems...
  22. ncbi Glycan analysis in cell culture-based influenza vaccine production: influence of host cell line and virus strain on the glycosylation pattern of viral hemagglutinin
    Jana Schwarzer
    Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany
    Vaccine 27:4325-36. 2009
    ..In contrast to this, a principal switch of the N-glycan type attached to HA was observed when comparing different virus types (A and B) and subtypes (H1N1 and H3N2)...
  23. ncbi Real-time RT-qPCR assay for the analysis of human influenza A virus transcription and replication dynamics
    Diana Vester
    Bioprocess Engineering, Otto von Guericke University Magdeburg, Magdeburg, Germany
    J Virol Methods 168:63-71. 2010
    ..9 hpi viral genome replication could be detected. The vRNA(-)s were synthesized in equimolar amounts and similar dynamics whereas preferential synthesis of NS1 vmRNA(+) in early transcription phases and a delay for M1 vmRNA(+) was found...
  24. ncbi Quantitative analysis of cellular proteome alterations in human influenza A virus-infected mammalian cell lines
    Diana Vester
    Bioprocess Engineering, Otto von Guericke University Magdeburg, Magdeburg, Germany
    Proteomics 9:3316-27. 2009
    ..Quantitative proteome-wide profiling of virus infection can provide insights into complexity and dynamics of virus-host cell interactions and may accelerate antiviral research and support optimization of vaccine manufacturing processes...
  25. pmc Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production
    Josef Schulze-Horsel
    Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany
    BMC Biotechnol 8:45. 2008
    ..Flow cytometry represents a valuable tool to investigate the influences of cultivation conditions and process variations on virus replication and virus yields...
  26. ncbi The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis
    Verena Lohr
    Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr, 1, 39106 Magdeburg, Germany
    BMC Biotechnol 14:72. 2014
    ..Therefore, we derived previously a continuous suspension cell line, AGE1.CR.pIX, from muscovy duck and established chemically-defined media for virus propagation...
  27. doi Production of high-titer human influenza A virus with adherent and suspension MDCK cells cultured in a single-use hollow fiber bioreactor
    Felipe Tapia
    Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr 1, 39106 Magdeburg, Germany Advanced Materials and Processes MAP, Elite Graduate Program, University of Erlangen Nuremberg, Martenstr 5 7, 91058 Erlangen, Germany Electronic address
    Vaccine 32:1003-11. 2014
    ....
  28. doi Vaccine production: upstream processing with adherent or suspension cell lines
    Yvonne Genzel
    Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany
    Methods Mol Biol 1104:371-93. 2014
    ..In particular, use of serum-containing, serum-free, and chemically defined media as well as choice of cultivation vessel are considered. ..
  29. ncbi High-performance anion-exchange chromatography using on-line electrolytic eluent generation for the determination of more than 25 intermediates from energy metabolism of mammalian cells in culture
    Joachim B Ritter
    Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstr 1, 39106 Magdeburg, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 843:216-26. 2006
    ..99 for most analytes. Working ranges were between 0.125-3.875 and 4.5-139.5 microM. Sample pH had a major impact on the quantification of several metabolites, but measurements were robust within a pH range of 6.5-9.0...
  30. ncbi The influence of cell growth and enzyme activity changes on intracellular metabolite dynamics in AGE1.HN.AAT cells
    Alexander G Rath
    Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering Group, Sandtorstr 1, 39106 Magdeburg, Germany Electronic address
    J Biotechnol 178:43-53. 2014
    ..An additional knockdown of the glutamine synthetase might help to prevent unnecessary dissipation of ATP, to yield a cell line with optimized growth characteristics and increased overall productivity. ..