B M Fuchs

Summary

Affiliation: Max Planck Institute for Marine Microbiology
Country: Germany

Publications

  1. doi request reprint Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques
    Rudolf Amann
    Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Celsiusstr 1, D 28359 Bremen, Germany
    Nat Rev Microbiol 6:339-48. 2008
  2. ncbi request reprint The effect of nucleobase-specific fluorescence quenching on in situ hybridization with rRNA-targeted oligonucleotide probes
    Sebastian Behrens
    Max Planck Institute of Marine Microbiology, Bremen, Germany
    Syst Appl Microbiol 27:565-72. 2004
  3. pmc Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov
    Stefan Spring
    Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures, Inhoffenstr 7B, Braunschweig 38124, Germany
    BMC Microbiol 13:118. 2013
  4. pmc Graphical representation of ribosomal RNA probe accessibility data using ARB software package
    Yadhu Kumar
    Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen, D 85350 Freising, Germany
    BMC Bioinformatics 6:61. 2005
  5. pmc Unlabeled helper oligonucleotides increase the in situ accessibility to 16S rRNA of fluorescently labeled oligonucleotide probes
    B M Fuchs
    Max Planck Institut für Marine Mikrobiologie, D 28359 Bremen, Germany
    Appl Environ Microbiol 66:3603-7. 2000
  6. pmc In situ accessibility of Escherichia coli 23S rRNA to fluorescently labeled oligonucleotide probes
    B M Fuchs
    Max Planck Institute for Marine Microbiology, D 28359 Bremen, Germany
    Appl Environ Microbiol 67:961-8. 2001
  7. ncbi request reprint Changes in community composition during dilution cultures of marine bacterioplankton as assessed by flow cytometric and molecular biological techniques
    B M Fuchs
    Max Planck Institut für Marine Mikrobiologie, Bremen, Germany
    Environ Microbiol 2:191-201. 2000
  8. pmc Characterization of a marine gammaproteobacterium capable of aerobic anoxygenic photosynthesis
    Bernhard M Fuchs
    Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D 28359 Bremen, Germany
    Proc Natl Acad Sci U S A 104:2891-6. 2007
  9. pmc Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes
    B M Fuchs
    Max Planck Institut für Marine Mikrobiologie, D 28359 Bremen, Germany
    Appl Environ Microbiol 64:4973-82. 1998
  10. ncbi request reprint The identification of microorganisms by fluorescence in situ hybridisation
    R Amann
    Molecular Ecology Group, Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D 28359, Bremen, Germany
    Curr Opin Biotechnol 12:231-6. 2001

Collaborators

Detail Information

Publications21

  1. doi request reprint Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques
    Rudolf Amann
    Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Celsiusstr 1, D 28359 Bremen, Germany
    Nat Rev Microbiol 6:339-48. 2008
    ....
  2. ncbi request reprint The effect of nucleobase-specific fluorescence quenching on in situ hybridization with rRNA-targeted oligonucleotide probes
    Sebastian Behrens
    Max Planck Institute of Marine Microbiology, Bremen, Germany
    Syst Appl Microbiol 27:565-72. 2004
    ..Probes quenched upon hybridization to a guanine-rich region of purified RNA in solution were not quenched upon FISH. Among other factors the high protein concentration within cells may prevent quenching of probe fluorescence in situ...
  3. pmc Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov
    Stefan Spring
    Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures, Inhoffenstr 7B, Braunschweig 38124, Germany
    BMC Microbiol 13:118. 2013
    ..Within this group a close relationship between aerobic anoxygenic photoheterotrophs and non-phototrophic members has been found...
  4. pmc Graphical representation of ribosomal RNA probe accessibility data using ARB software package
    Yadhu Kumar
    Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen, D 85350 Freising, Germany
    BMC Bioinformatics 6:61. 2005
    ..Careful in silico design and evaluation of potential oligonucleotide probe targets is therefore crucial for performing successful hybridization experiments...
  5. pmc Unlabeled helper oligonucleotides increase the in situ accessibility to 16S rRNA of fluorescently labeled oligonucleotide probes
    B M Fuchs
    Max Planck Institut für Marine Mikrobiologie, D 28359 Bremen, Germany
    Appl Environ Microbiol 66:3603-7. 2000
    ..We conclude that helpers can open inaccessible rRNA regions for FISH with oligonucleotide probes and will thereby further improve the applicability of this technique for in situ identification of microorganisms...
  6. pmc In situ accessibility of Escherichia coli 23S rRNA to fluorescently labeled oligonucleotide probes
    B M Fuchs
    Max Planck Institute for Marine Microbiology, D 28359 Bremen, Germany
    Appl Environ Microbiol 67:961-8. 2001
    ..coli, which may be extrapolated to other bacteria. Thereby, it may contribute to a better exploitation of the high potential of the 23S rRNA for identification of bacteria in the future...
  7. ncbi request reprint Changes in community composition during dilution cultures of marine bacterioplankton as assessed by flow cytometric and molecular biological techniques
    B M Fuchs
    Max Planck Institut für Marine Mikrobiologie, Bremen, Germany
    Environ Microbiol 2:191-201. 2000
    ..Furthermore, it was demonstrated that the combination of flow cytometric analysis and sorting combined with FISH and DGGE analysis presented a fairly rapid method of analysing the taxonomic composition of marine bacterioplankton...
  8. pmc Characterization of a marine gammaproteobacterium capable of aerobic anoxygenic photosynthesis
    Bernhard M Fuchs
    Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D 28359 Bremen, Germany
    Proc Natl Acad Sci U S A 104:2891-6. 2007
    ..KT71 enables future experiments investigating the importance of this group of gammaproteobacterial AAnPs in coastal environments...
  9. pmc Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes
    B M Fuchs
    Max Planck Institut für Marine Mikrobiologie, D 28359 Bremen, Germany
    Appl Environ Microbiol 64:4973-82. 1998
    ..Considering the high evolutionary conservation of 16S rRNA, the in situ accessibility map of E. coli should facilitate a more rational selection of probe target sites for other species as well...
  10. ncbi request reprint The identification of microorganisms by fluorescence in situ hybridisation
    R Amann
    Molecular Ecology Group, Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D 28359, Bremen, Germany
    Curr Opin Biotechnol 12:231-6. 2001
    ..Over the past year there have been a number of methodological developments in this area and new applications of FISH in microbial ecology and biotechnology have been reported...
  11. pmc Temporal variability of coastal Planctomycetes clades at Kabeltonne station, North Sea
    Ilaria Pizzetti
    Max Planck Institute for Marine Microbiology, Celsiusstr 1, D 28359 Bremen, Germany
    Appl Environ Microbiol 77:5009-17. 2011
    ..Our analyses suggest that the seasonal succession of the Planctomycetes is correlated with algal blooms. We hypothesize that the niche partitioning of the different clades might be caused by their algal substrates...
  12. ncbi request reprint Microbial control of phosphate in the nutrient-depleted North Atlantic subtropical gyre
    Mikhail V Zubkov
    National Oceanography Centre, Southampton SO143ZH, UK
    Environ Microbiol 9:2079-89. 2007
    ..3%) played minor roles in direct phosphate uptake. We have demonstrated that phosphate uptake in the oligotrophic gyre is rapid and dominated by two bacterial groups rather than by algae...
  13. pmc Potential interactions of particle-associated anammox bacteria with bacterial and archaeal partners in the Namibian upwelling system
    Dagmar Woebken
    Max Planck Institute for Marine Microbiology, Molecular Ecology, Celsiusstr 1, 28359 Bremen, Germany
    Appl Environ Microbiol 73:4648-57. 2007
    ....
  14. pmc In situ accessibility of small-subunit rRNA of members of the domains Bacteria, Archaea, and Eucarya to Cy3-labeled oligonucleotide probes
    Sebastian Behrens
    Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D 28359 Bremen, Germany
    Appl Environ Microbiol 69:1748-58. 2003
    ..Finally, the 16S rRNA consensus model was compared to data on the in situ accessibility of the 18S rRNA of S. cerevisiae...
  15. pmc Massive nitrogen loss from the Benguela upwelling system through anaerobic ammonium oxidation
    Marcel M M Kuypers
    Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany
    Proc Natl Acad Sci U S A 102:6478-83. 2005
    ..We hypothesize that anammox could also be responsible for substantial nitrogen loss from other OMZ waters of the ocean...
  16. ncbi request reprint Fluorescence in situ hybridization of 16S rRNA gene clones (Clone-FISH) for probe validation and screening of clone libraries
    Andreas Schramm
    Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195 2700, USA
    Environ Microbiol 4:713-20. 2002
    ..In summary, Clone-FISH is a simple and fast technique, compatible with a wide variety of cloning vectors and hosts, that should have general utility for probe validation and screening of clone libraries...
  17. pmc High rate of uptake of organic nitrogen compounds by Prochlorococcus cyanobacteria as a key to their dominance in oligotrophic oceanic waters
    Mikhail V Zubkov
    Southampton Oceanography Centre, Southampton SO14 3ZH, United Kingdom
    Appl Environ Microbiol 69:1299-304. 2003
    ..This finding may provide a mechanism for Prochlorococcus' competitive dominance over both strictly autotrophic algae and other bacteria in oligotrophic regions sustained by nutrient remineralization via a microbial loop...
  18. pmc In situ accessibility of Saccharomyces cerevisiae 26S rRNA to Cy3-labeled oligonucleotide probes comprising the D1 and D2 domains
    João Inácio
    Centro de Recursos Microbiológicos, Biotechnology Unit, Faculty of Sciences and Technology, New University of Lisbon, 2829 516 Caparica, Portugal
    Appl Environ Microbiol 69:2899-905. 2003
    ..This work contributes to a more rational design of species-specific probes for yeast identification and monitoring...
  19. pmc Flow sorting of marine bacterioplankton after fluorescence in situ hybridization
    Raju Sekar
    Max Planck Institut für Marine Mikrobiologie, Celsiusstrasse 1, D 28359 Bremen, Germany
    Appl Environ Microbiol 70:6210-9. 2004
    ..This illustrates that a combination of CARD-FISH and flow sorting might be a powerful approach to study the diversity and potentially the activity and the genomes of different bacterial populations in aquatic habitats...
  20. pmc SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB
    Elmar Pruesse
    Microbial Genomics Group, Max Planck Institute for Marine Microbiology
    Nucleic Acids Res 35:7188-96. 2007
    ..The latest publicly available database release 91 (August 2007) hosts 547 521 sequences split into 461 823 small subunit and 85 689 large subunit rRNAs...
  21. pmc Is the in situ accessibility of the 16S rRNA of Escherichia coli for Cy3-labeled oligonucleotide probes predicted by a three-dimensional structure model of the 30S ribosomal subunit?
    Sebastian Behrens
    Max Planck Institute of Marine Microbiology, Bremen Max Planck Institute of Molecular Genetics, Berlin, Germany
    Appl Environ Microbiol 69:4935-41. 2003
    ..The presence or absence of the strongly denaturing detergent sodium dodecyl sulfate had a much more pronounced effect than a change of fixative from paraformaldehyde to ethanol...