K Büssow

Summary

Affiliation: Max Planck Institute for Molecular Genetics
Country: Germany

Publications

  1. pmc Vectors for co-expression of an unrestricted number of proteins
    Christoph Scheich
    Max Planck Institute for Molecular Genetics, Department of Vertebrate Genomics, Berlin, Germany
    Nucleic Acids Res 35:e43. 2007
  2. pmc Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones
    Konrad Büssow
    Protein Structure Factory, Heubnerweg 6, 14059 Berlin, Germany
    Microb Cell Fact 4:21. 2005
  3. pmc Fast identification of folded human protein domains expressed in E. coli suitable for structural analysis
    Christoph Scheich
    Proteinstrukturfabrik, Heubnerweg 6, 14059 Berlin, Germany
    BMC Struct Biol 4:4. 2004
  4. pmc X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1)
    Babu A Manjasetty
    Protein Structure Factory, c o BESSY GmbH, Albert Einstein Str 15, 12489 Berlin, Germany
    BMC Struct Biol 5:21. 2005
  5. pmc An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
    Christoph Scheich
    Protein Structure Factory, Max Planck Institute of Molecular Genetics, Heubnerweg 6, 14059 Berlin, Germany
    BMC Biotechnol 3:12. 2003
  6. ncbi request reprint Protein in gels, computers, crystals and camels
    K Büssow
    Max Planck Institute of Molecular Genetics, Berlin, Germany
    Trends Biotechnol 19:328-9. 2001
  7. ncbi request reprint A human cDNA library for high-throughput protein expression screening
    K Büssow
    Max Planck Institute of Molecular Genetics, Ihnestrasse 73, Berlin, 14195, Germany
    Genomics 65:1-8. 2000
  8. ncbi request reprint Protein array technology. Potential use in medical diagnostics
    K Büssow
    Max Planck Institute of Molecular Genetics, Berlin, Germany
    Am J Pharmacogenomics 1:37-43. 2001
  9. pmc A catalog of human cDNA expression clones and its application to structural genomics
    Konrad Büssow
    Protein Structure Factory, Heubnerweg 6, 14059 Berlin, Germany
    Genome Biol 5:R71. 2004
  10. pmc ORFer--retrieval of protein sequences and open reading frames from GenBank and storage into relational databases or text files
    Konrad Büssow
    Protein Structure Factory, Max Planck Institute of Molecular Genetics, Heubnerweg 6, 14059 Berlin, Germany
    BMC Bioinformatics 3:40. 2002

Collaborators

Detail Information

Publications15

  1. pmc Vectors for co-expression of an unrestricted number of proteins
    Christoph Scheich
    Max Planck Institute for Molecular Genetics, Department of Vertebrate Genomics, Berlin, Germany
    Nucleic Acids Res 35:e43. 2007
    ..It is expected that applications are not restricted to bacteria, but could include co-expression in other hosts such as Bacluovirus/insect cells...
  2. pmc Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones
    Konrad Büssow
    Protein Structure Factory, Heubnerweg 6, 14059 Berlin, Germany
    Microb Cell Fact 4:21. 2005
    ..139 human proteins (18%) could be expressed and purified in soluble form and with the expected size. All E. coli expression clones are publicly available to facilitate further functional characterisation of this set of human proteins...
  3. pmc Fast identification of folded human protein domains expressed in E. coli suitable for structural analysis
    Christoph Scheich
    Proteinstrukturfabrik, Heubnerweg 6, 14059 Berlin, Germany
    BMC Struct Biol 4:4. 2004
    ..For this purpose, methods need to be implemented for fast and reliable screening of the expressed proteins as early as possible in the overall process from cloning to structure determination...
  4. pmc X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1)
    Babu A Manjasetty
    Protein Structure Factory, c o BESSY GmbH, Albert Einstein Str 15, 12489 Berlin, Germany
    BMC Struct Biol 5:21. 2005
    ..Human Aortic Preferentially Expressed Protein-1 (APEG-1) is a novel specific smooth muscle differentiation marker thought to play a role in the growth and differentiation of arterial smooth muscle cells (SMCs)...
  5. pmc An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
    Christoph Scheich
    Protein Structure Factory, Max Planck Institute of Molecular Genetics, Heubnerweg 6, 14059 Berlin, Germany
    BMC Biotechnol 3:12. 2003
    ..Such studies require methods to express and purify these proteins in a parallel, time and cost effective manner...
  6. ncbi request reprint Protein in gels, computers, crystals and camels
    K Büssow
    Max Planck Institute of Molecular Genetics, Berlin, Germany
    Trends Biotechnol 19:328-9. 2001
    ..The IBC's Proteomics 2001 meeting was held in Philadelphia, PA, USA, 14-17 May 2000...
  7. ncbi request reprint A human cDNA library for high-throughput protein expression screening
    K Büssow
    Max Planck Institute of Molecular Genetics, Ihnestrasse 73, Berlin, 14195, Germany
    Genomics 65:1-8. 2000
    ..Our data indicate that high-throughput screening of an arrayed protein expression library is an economical way of generating large numbers of clones producing recombinant human proteins for structural and functional analyses...
  8. ncbi request reprint Protein array technology. Potential use in medical diagnostics
    K Büssow
    Max Planck Institute of Molecular Genetics, Berlin, Germany
    Am J Pharmacogenomics 1:37-43. 2001
    ..Microarrays can now be used to go from an individual clone to a specific gene and its protein product. Clone libraries become amenable to database integration including all steps from DNA sequencing to functional assays of gene products...
  9. pmc A catalog of human cDNA expression clones and its application to structural genomics
    Konrad Büssow
    Protein Structure Factory, Heubnerweg 6, 14059 Berlin, Germany
    Genome Biol 5:R71. 2004
    ..Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures...
  10. pmc ORFer--retrieval of protein sequences and open reading frames from GenBank and storage into relational databases or text files
    Konrad Büssow
    Protein Structure Factory, Max Planck Institute of Molecular Genetics, Heubnerweg 6, 14059 Berlin, Germany
    BMC Bioinformatics 3:40. 2002
    ..Functional genomics involves the parallel experimentation with large sets of proteins. This requires management of large sets of open reading frames as a prerequisite of the cloning and recombinant expression of these proteins...
  11. pmc A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library
    K Büssow
    Max Planck Institute for Molecular Genetics, Ihnestr 73, D 14195 Berlin, Germany
    Nucleic Acids Res 26:5007-8. 1998
    ..His antibody, Qiagen) detected 20% of the library as putative expression clones. Two example genes, GAPDH and HSP90alpha, were identified on high-density filters using DNA probes and antibodies against their proteins...
  12. ncbi request reprint Large-gel two-dimensional electrophoresis-matrix assisted laser desorption/ionization-time of flight-mass spectrometry: an analytical challenge for studying complex protein mixtures
    E Nordhoff
    Department Lehrach, Max Planck Institute for Molecular Genetics, Berlin, Germany
    Electrophoresis 22:2844-55. 2001
    ..This challenge is heading a second part of the paper...
  13. ncbi request reprint Alpha-cyano-4-hydroxycinnamic acid affinity sample preparation. A protocol for MALDI-MS peptide analysis in proteomics
    J Gobom
    Max Planck Institute for Molecular Genetics, Berlin, Germany
    Anal Chem 73:434-8. 2001
    ..Performance of the sample preparation is demonstrated with in situ proteolytic digests of human brain proteins separated by two-dimensional gel electrophoresis...
  14. pmc Rapid determination of short DNA sequences by the use of MALDI-MS
    E Nordhoff
    Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany
    Nucleic Acids Res 28:E86. 2000
    ..The mass spectrometric analyses and subsequent sequence read-out require only a few seconds per template...
  15. ncbi request reprint Identification of the predominant antigenic epitopes in intestinal flora in IBD
    A Ergin
    Medizinische Klinik I, Universitatsmedizin Berlin, Charite, Campus Benjamin Franklin, Hindenburgdamm, Berlin, Germany
    Mucosal Immunol 1:S19-23. 2008
    ..The reason for the decreased frequency of an in vitro T(H)-cell IFNgamma response toward E. coli proteins in peripheral blood of CD and AS patients, e.g., increased suppression needs to be clarified...