Hedda Wardemann

Summary

Affiliation: Max-Planck-Institute for Infection Biology
Country: Germany

Publications

  1. pmc Development of self-reactive germinal center B cells and plasma cells in autoimmune Fc gammaRIIB-deficient mice
    Thomas Tiller
    Max Planck Molecular Immunology Research Group, Max Planck Institute for Infection Biology, 10117 Berlin, Germany
    J Exp Med 207:2767-78. 2010
  2. pmc Atypical and classical memory B cells produce Plasmodium falciparum neutralizing antibodies
    Matthias F Muellenbeck
    Max Planck Research Group Molecular Immunology, Max Planck Institute for Infection Biology, 10117 Berlin, Germany
    J Exp Med 210:389-99. 2013
  3. pmc Persistent expression of autoantibodies in SLE patients in remission
    Sergey Yurasov
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA
    J Exp Med 203:2255-61. 2006
  4. doi request reprint Expression cloning of human B cell immunoglobulins
    Hedda Wardemann
    Research Group Molecular Immunology, Max Planck Institute for Infection Biology, Berlin, Germany
    Methods Mol Biol 971:93-111. 2013
  5. pmc T cell-independent B cell activation induces immunosuppressive sialylated IgG antibodies
    Constanze Hess
    Laboratory of Tolerance and Autoimmunity, German Rheumatism Research Center, Berlin, Germany
    J Clin Invest 123:3788-96. 2013
  6. pmc Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning
    Thomas Tiller
    Max Planck Institute for Infection Biology, D 10117 Berlin, Germany
    J Immunol Methods 329:112-24. 2008
  7. pmc Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligation
    Hugo Mouquet
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10065, USA
    Nature 467:591-5. 2010
  8. pmc Autoreactivity in human IgG+ memory B cells
    Thomas Tiller
    Max Planck Institute for Infection Biology, D 10117 Berlin, Germany
    Immunity 26:205-13. 2007
  9. pmc A method for identification of HIV gp140 binding memory B cells in human blood
    Johannes F Scheid
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10065, USA
    J Immunol Methods 343:65-7. 2009
  10. pmc Autoreactive IgG memory antibodies in patients with systemic lupus erythematosus arise from nonreactive and polyreactive precursors
    Brun Mietzner
    Max Planck Institute for Infection Biology, 10117 Berlin, Germany
    Proc Natl Acad Sci U S A 105:9727-32. 2008

Collaborators

Detail Information

Publications29

  1. pmc Development of self-reactive germinal center B cells and plasma cells in autoimmune Fc gammaRIIB-deficient mice
    Thomas Tiller
    Max Planck Molecular Immunology Research Group, Max Planck Institute for Infection Biology, 10117 Berlin, Germany
    J Exp Med 207:2767-78. 2010
    ....
  2. pmc Atypical and classical memory B cells produce Plasmodium falciparum neutralizing antibodies
    Matthias F Muellenbeck
    Max Planck Research Group Molecular Immunology, Max Planck Institute for Infection Biology, 10117 Berlin, Germany
    J Exp Med 210:389-99. 2013
    ..Understanding the memory B cell response to natural Pf infection may be key to the development of a malaria vaccine that induces long-lived protection...
  3. pmc Persistent expression of autoantibodies in SLE patients in remission
    Sergey Yurasov
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA
    J Exp Med 203:2255-61. 2006
    ..Our finding that abnormal levels of self-reactive mature naive B cells persist in the majority of patients in clinical remission suggests that early checkpoint abnormalities are an integral feature of SLE...
  4. doi request reprint Expression cloning of human B cell immunoglobulins
    Hedda Wardemann
    Research Group Molecular Immunology, Max Planck Institute for Infection Biology, Berlin, Germany
    Methods Mol Biol 971:93-111. 2013
    ..The strategy may be used to obtain information on the clonal evolution of B cell lymphomas by single cell Ig gene sequencing and on the antibody reactivity of human lymphoma B cells...
  5. pmc T cell-independent B cell activation induces immunosuppressive sialylated IgG antibodies
    Constanze Hess
    Laboratory of Tolerance and Autoimmunity, German Rheumatism Research Center, Berlin, Germany
    J Clin Invest 123:3788-96. 2013
    ....
  6. pmc Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning
    Thomas Tiller
    Max Planck Institute for Infection Biology, D 10117 Berlin, Germany
    J Immunol Methods 329:112-24. 2008
    ....
  7. pmc Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligation
    Hugo Mouquet
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10065, USA
    Nature 467:591-5. 2010
    ..Despite the low affinity of the polyreactive combining site, heteroligation demonstrably increases the apparent affinity of polyreactive antibodies to HIV...
  8. pmc Autoreactivity in human IgG+ memory B cells
    Thomas Tiller
    Max Planck Institute for Infection Biology, D 10117 Berlin, Germany
    Immunity 26:205-13. 2007
    ..We conclude that deregulation of self-reactive IgG(+) memory B cells may be associated with autoimmunity...
  9. pmc A method for identification of HIV gp140 binding memory B cells in human blood
    Johannes F Scheid
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10065, USA
    J Immunol Methods 343:65-7. 2009
    ..When biotinylated gp140 is used to stain peripheral blood mononuclear cells it identifies a distinct population of gp140 binding B cells by flow cytometry...
  10. pmc Autoreactive IgG memory antibodies in patients with systemic lupus erythematosus arise from nonreactive and polyreactive precursors
    Brun Mietzner
    Max Planck Institute for Infection Biology, 10117 Berlin, Germany
    Proc Natl Acad Sci U S A 105:9727-32. 2008
    ..Heterogeneity in the frequency of memory B cells expressing SLE-associated autoantibodies suggests that this variable may be important in the outcome of therapies that ablate this compartment...
  11. pmc A checkpoint for autoreactivity in human IgM+ memory B cell development
    Makoto Tsuiji
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA
    J Exp Med 203:393-400. 2006
    ..Selection against self-reactive antibodies was implemented before the onset of somatic hypermutation. We conclude that a third checkpoint selects against self-reactivity during IgM+ memory B cell development in humans...
  12. pmc Defective B cell tolerance checkpoints in systemic lupus erythematosus
    Sergey Yurasov
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY, USA
    J Exp Med 201:703-11. 2005
    ..We conclude that SLE is associated with abnormal early B cell tolerance...
  13. doi request reprint Tolerance induction with T cell-dependent protein antigens induces regulatory sialylated IgGs
    Carolin M Oefner
    Laboratory of Tolerance and Autoimmunity, German Rheumatism Research Center, Leibniz Institute, Berlin, Germany
    J Allergy Clin Immunol 129:1647-55.e13. 2012
    ..Under both conditions, IgG antibodies are generated, but whether they have different immunoregulatory functions remains elusive...
  14. pmc Differential regulation of self-reactivity discriminates between IgG+ human circulating memory B cells and bone marrow plasma cells
    Johannes F Scheid
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 108:18044-8. 2011
    ....
  15. ncbi request reprint B-cell self-tolerance in humans
    Hedda Wardemann
    Max Planck Institute for Infection Biology, Campus Charite Mitte, Schumannstrasse 21 22, D 10117 Berlin, Germany
    Adv Immunol 95:83-110. 2007
    ..Here we summarize recent data on the self-reactive B-cell repertoire in healthy humans and in patients with autoimmunity...
  16. doi request reprint B-cell tolerance checkpoints in health and autoimmunity
    Eric Meffre
    Hospital for Special Surgery, Weill Medical College of Cornell University, New York, NY 10021, USA
    Curr Opin Immunol 20:632-8. 2008
    ..Under normal circumstances, several mechanisms regulate the removal of autoreactive B cells and defects in central and peripheral B cell tolerance checkpoints are associated with the development of autoimmunity in humans...
  17. ncbi request reprint Cloning and expression of murine Ig genes from single B cells
    Thomas Tiller
    Max Planck Institute for Infection Biology, Chariteplatz 1, Berlin D 10117, Germany
    J Immunol Methods 350:183-93. 2009
    ....
  18. pmc Human autoantibody silencing by immunoglobulin light chains
    Hedda Wardemann
    The Rockefeller University, 1230 York Ave, Box 220, New York, NY 10021, USA
    J Exp Med 200:191-9. 2004
    ..The greater efficacy of Iglambda chains as silencer of autoreactivity provides a possible explanation for the expansion and altered configuration of the Iglambda locus in evolution...
  19. pmc Bruton's tyrosine kinase is essential for human B cell tolerance
    Yen Shing Ng
    Laboratory of Biochemistry and Molecular Immunology, The Hospital for Special Surgery, New York, NY 10021, USA
    J Exp Med 200:927-34. 2004
    ..Nevertheless, XLA B cells are enriched in autoreactive clones. Our results demonstrate that Btk is essential in regulating thresholds for human B cell tolerance...
  20. doi request reprint TLR9 in peritoneal B-1b cells is essential for production of protective self-reactive IgM to control Th17 cells and severe autoimmunity
    Alexander D Stoehr
    Laboratory of Tolerance and Autoimmunity, German Rheumatism Research Center, Leibniz Institute, 10117 Berlin, Germany
    J Immunol 187:2953-65. 2011
    ..Taken together, these data provide evidence for a TLR9-dependent tolerance mechanism of peritoneal B-1b cells generating protective self-reactive IgM in lupus-prone mice to control Th17 cell development and severe autoimmunity...
  21. ncbi request reprint RAGs and regulation of autoantibodies
    Mila Jankovic
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA
    Annu Rev Immunol 22:485-501. 2004
    ..Here we review the prevalence of autoantibodies in the initial antibody repertoire, their regulation by receptor editing, and the role of the recombinase proteins (RAG1 and RAG2) in this process...
  22. pmc Surrogate light chain expressing human peripheral B cells produce self-reactive antibodies
    Eric Meffre
    Laboratory of Molecular Immunology, The Rockefeller University, 1230 York Ave, New York, NY 10021, USA
    J Exp Med 199:145-50. 2004
    ..We conclude that V-preB+L+ B cells are a unique subset of normal circulating human B cells that escape central tolerance mechanisms and express self-reactive antibodies including potentially harmful ANAs...
  23. ncbi request reprint Predominant autoantibody production by early human B cell precursors
    Hedda Wardemann
    Laboratory of Molecular Immunology, Rockefeller University, New York, NY 10021, USA
    Science 301:1374-7. 2003
    ..Most of these autoantibodies were removed from the population at two discrete checkpoints during B cell development. Inefficient checkpoint regulation would lead to substantial increases in circulating autoantibodies...
  24. ncbi request reprint B-cell tolerance checkpoints in healthy humans and patients with systemic lupus erythematosus
    Sergey Yurasov
    Laboratory of Molecular Immunology, The Rockefeller University, 1230 York Ave, New York, NY 10021, USA
    Ann N Y Acad Sci 1062:165-74. 2005
    ....
  25. pmc Unmutated and mutated chronic lymphocytic leukemias derive from self-reactive B cell precursors despite expressing different antibody reactivity
    Maxime Hervé
    Laboratory of Biochemistry and Molecular Immunology, Hospital for Special Surgery, New York, New York 10021, USA
    J Clin Invest 115:1636-43. 2005
    ..We concluded that both UM-CLLs and M-CLLs originate from self-reactive B cell precursors and that SHM plays an important role in the development of the disease by altering original BCR autoreactivity...
  26. pmc The majority of intestinal IgA+ and IgG+ plasmablasts in the human gut are antigen-specific
    Julia Benckert
    Max Planck Research Group Molecular Immunology, Max Planck Institute for Infection Biology, Berlin, Germany
    J Clin Invest 121:1946-55. 2011
    ....
  27. doi request reprint Broad diversity of neutralizing antibodies isolated from memory B cells in HIV-infected individuals
    Johannes F Scheid
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10065, USA
    Nature 458:636-40. 2009
    ..Thus, the IgG memory B-cell compartment in the selected group of patients with broad serum neutralizing activity to HIV is comprised of multiple clonal responses with neutralizing activity directed against several epitopes on gp120...
  28. ncbi request reprint Single-cell based high-throughput sequencing of full-length immunoglobulin heavy and light chain genes
    Christian E Busse
    Research Group Molecular Immunology, Max Planck Institute for Infection Biology, Berlin, Germany
    Eur J Immunol 44:597-603. 2014
    ....
  29. ncbi request reprint Visualizing dendritic cell networks in vivo
    Randall L Lindquist
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA
    Nat Immunol 5:1243-50. 2004
    ..Mature DCs were more motile than steady-state DCs and were rapidly dispersed and integrated into the sessile network, facilitating their interaction with migrating T cells...