P R Jungblut
Affiliation: Max-Planck-Institute for Infection Biology
- Proteomics in human disease: cancer, heart and infectious diseasesP R Jungblut
Max Planck Institut für Infektionsbiologie, Protein Analyse Einheit, Berlin, Germany
Electrophoresis 20:2100-10. 1999..Similarly, antibody reactivity to seven different marker antigens of T. gondii allowed differentiation between acute and latent toxoplasmosis, an important diagnostic tool in both pregnancy and immunosuppressed patients...
- Proteomic identification of secreted proteins of Propionibacterium acnesCarsten Holland
Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany
BMC Microbiol 10:230. 2010..acnes virulence factors remains scarce. Here, we identified proteins secreted during anaerobic cultivation of a range of skin and clinical P. acnes isolates, spanning the four known phylogenetic groups...
- Calibration of mass spectrometric peptide mass fingerprint data without specific external or internal calibrantsWitold E Wolski
Max Planck Institute for Molecular Genetics, Ihnestrasse 63 73, D 14195 Berlin, Germany
BMC Bioinformatics 6:203. 2005..It relies on the comparison between experimentally determined and theoretical mass spectra. The PMF process requires calibration, usually performed with external or internal calibrants of known molecular masses...
- Helicobacter pylori proteomics by 2-DE/MS, 1-DE-LC/MS and functional data miningPeter R Jungblut
Core Facility Protein Analysis, Max Planck Institute for Infection Biology, 10117 Berlin, Germany
Proteomics 10:182-93. 2010..Access to the PROMPT-generated diagrams in the Proteome Database allows the mining of experimental data with respect to other functional aspects...
- Towards the analysis of protein species: an overview about strategies and methodsPeter R Jungblut
Core Facility Protein Analysis, Max Planck Institute for Infection Biology, Chariteplatz 1, 10117, Berlin, Germany
Amino Acids 41:219-22. 2011..For determining the function of defined protein species a closer cooperation between cell biologists and proteomics experts is desirable...
- Proteome analysis of bacterial pathogensP R Jungblut
Max Planck Institute for Infection Biology, Support Unit for Biochemistry, Schumann Str 21 22, 10117, Berlin, Germany
Microbes Infect 3:831-40. 2001..Immunoproteomics identifies specific and nonspecific antigens. For the management of the huge data amounts, bioinformatics is a valuable instrument for the construction of complex protein databases...
- Comparative proteome analysis of Helicobacter pyloriP R Jungblut
Max Planck Institute for Infection Biology, Central Support Unit Biochemistry, Berlin, Germany
Mol Microbiol 36:710-25. 2000..This basic knowledge of the proteome in the public domain will be an effective instrument for the identification of new virulence or pathogenic factors, and antigens of potentially diagnostic or curative value against H. pylori...
- Comprehensive detection of immunorelevant Borrelia garinii antigens by two-dimensional electrophoresisP R Jungblut
Institute of Microbiology, Medical Faculty, University Innsbruck, Austria
Electrophoresis 20:3611-22. 1999..A 2-DE database of B. garinii was built up and is available on the World Wide Web (http://www.mpiib-berlin.mpg.de/2D-PAGE)...
- Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains: towards functional genomics of microbial pathogensP R Jungblut
Protein Analysis Unit, Max Planck Institute for Infection Biology, Berlin, Germany
Mol Microbiol 33:1103-17. 1999..It is to be hoped that the availability of the mycobacterial proteome will facilitate the design of novel measures for prevention and therapy of one of the great health threats, tuberculosis...
- Identification of proteins from Mycobacterium tuberculosis missing in attenuated Mycobacterium bovis BCG strainsJ Mattow
Max-Planck-Institute for Infection Biology, Berlin, Germany
Electrophoresis 22:2936-46. 2001..bovis BCG. The remaining 20 spots unique for M. tuberculosis were identified as proteins encoded by genes that are not known to be missing in M. bovis BCG...
- A dynamic two-dimensional polyacrylamide gel electrophoresis database: the mycobacterial proteome via InternetH J Mollenkopf
Max Planck Institute for Infection Biology, Department of Immunology, Berlin, Germany
Electrophoresis 20:2172-80. 1999..Furthermore, the database facilitates not only interactive use, but also worldwide active participation of other scientific groups with their own data, requiring only minimal computer hardware and knowledge of information technology...
- Proteome analysis of the common human pathogen Helicobacter pyloriD Bumann
Max Planck Institute for Infection Biology, Department of Molecular Biology, Schumannstrasse 21 22, 10117 Berlin, Germany
Proteomics 1:473-9. 2001..The results that have been obtained led to a more detailed understanding of the Helicobacter biology and pathology and open further interesting fields for future work...
- Identification of a tyrosine-phosphorylated 35 kDa carboxy-terminal fragment (p35CagA) of the Helicobacter pylori CagA protein in phagocytic cells: processing or breakage?S Moese
Max Planck Institut für Infektionsbiologie, Abt, Molekulare Biologie, Schumannstr 20 21, D 10117 Berlin, Germany
Proteomics 1:618-29. 2001..The possible function of CagA in host signal transduction and development of gastric disease is discussed...
- Proteomics reveals open reading frames in Mycobacterium tuberculosis H37Rv not predicted by genomicsP R Jungblut
Core Facility for Protein Analysis, Max Planck Institute for Infection Biology, Berlin, Germany
Infect Immun 69:5905-7. 2001....
- Identification of acidic, low molecular mass proteins of Mycobacterium tuberculosis strain H37Rv by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometryJ Mattow
Max Planck Institute for Infection Biology, Department of Immunology, Schumannstr 21 22, D 10117 Berlin, Germany
Proteomics 1:494-507. 2001..Six additional spots were shown to comprise proteins encoded by open reading frames that have not been predicted for M. tuberculosis H37Rv by genomic investigations...
- Analysis of missed cleavage sites, tryptophan oxidation and N-terminal pyroglutamylation after in-gel tryptic digestionB Thiede
Max Planck Institut für Infektionsbiologie, Monbijoustrasse 2, D 10117 Berlin, Germany
Rapid Commun Mass Spectrom 14:496-502. 2000..These data can be used for the refinement of protein searches by peptide mass fingerprinting...
- Matrix-assisted laser desorption-ionization mass spectrometry peptide mass fingerprinting for proteome analysis: identification efficiency after on-blot or in-gel digestion with and without desalting proceduresS Lamer
Max-Planck-Institute for Infection Biology, Central Support Unit Biochemistry, Berlin, Germany
J Chromatogr B Biomed Sci Appl 752:311-22. 2001..Whereas higher signal intensities resulted after concentration, hydrophilic peptides are better detected by direct measurement of the peptide mix without POROS R2 concentration...
- The necessity of functional proteomics: protein species and molecular function elucidation exemplified by in vivo alpha A crystallin N-terminal truncationW Hoehenwarter
Max Planck Institute for Infection Biology, Core Facility Protein Analysis, Berlin, Germany
Amino Acids 31:317-23. 2006..We assess the present state of technology and suggest a shift in resources and paradigm for the routine attainment of the protein species level in proteomics...
- Immunization with gp96 from Listeria monocytogenes-infected mice is due to N-formylated listerial peptidesA M Sponaas
Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany
J Immunol 167:6480-6. 2001..We assume a unique role of gp96 in Ag processing through the H2-M3 pathway...
- Eye lens proteomicsW Hoehenwarter
Max Planck Institute for Infection Biology, Core Facility Protein Analysis, Berlin, Germany
Amino Acids 30:369-89. 2006..Proteomics methodology is therefore ideal to further comprehensive understanding of this organ and the factors involved in cataractogenesis...
- Tyrosine phosphorylation patterns and size modification of the Helicobacter pylori CagA protein after translocation into gastric epithelial cellsS Backert
, Abt. Molekulare Biologie, Schumannstr. 20/21, D-10117 Berlin, Germany
Proteomics 1:608-17. 2001..Translocation, tyrosine phosphorylation and size modification of CagA might be involved in host signal transduction and development of gastric disease...
- Separation and identification of human heart proteinsD Jager
Department of Medicine III, Martin Luther University, Halle Wittenberg, Germany
J Chromatogr B Analyt Technol Biomed Life Sci 771:131-53. 2002..In concert with genomic data functional proteomics will hold the key for a better understanding and therapeutical management of cardiovascular diseases in the future...
- Proteome analysis of rat hepatomas: carcinogen-dependent tumor-associated protein variantsE Zeindl-Eberhart
Institute of Pathology, University of Munich, Germany
Electrophoresis 22:3009-18. 2001..The hypothesis is proposed that carcinogens leave specific fingerprints at the proteome level of manifest liver tumors...
- Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactionsA Villarino
Unité de Biochimie Structurale URA 2185 CNRS, Institut Pasteur, Paris, France
J Mol Biol 350:953-63. 2005..The ensuing model of PknB-GarA interactions suggests a substrate recruitment mechanism that might apply to other mycobacterial kinases bearing multiple phosphorylation sites in their activation loops...