Research Topics
| Iain W MattajSummaryCountry: Germany Publications
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Publications
Identification of 40LoVe, a Xenopus hnRNP D family protein involved in localizing a TGF-beta-related mRNA during oogenesisKevin Czaplinski
EMBL Gene Expression Programme, Meyerhofstrasse 1, Heidelberg 69117, Germany
Dev Cell 8:505-15. 2005..Our results associate an hnRNP D protein with mRNA localization and have implications for several functions mediated by this important protein family...- Identity elements used in export of mRNAsMutsuhito Ohno
The Institute for Virus Research, Laboratory of Biochemistry, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
Mol Cell 9:659-71. 2002..These data provide insight into mechanisms for discrimination of different classes of nuclear RNA and demonstrate that two RNAs of identical sequence can have distinct cytoplasmic fates depending on their mode of export... 
A compartmentalized phosphorylation/dephosphorylation system that regulates U snRNA export from the nucleusSaori Kitao
Institute for Virus Research, Kyoto University, Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
Mol Cell Biol 28:487-97. 2008..This finding was surprising in that such a specific system for U snRNA export regulation is composed of two such universal regulators, suggesting that this compartmentalized system is used more broadly for gene expression regulation...- Importin alpha associates with membranes and participates in nuclear envelope assembly in vitroVirginie Hachet
European Molecular Biology Laboratory, Meyerhofstrasse, Heidelberg, Germany
EMBO J 23:1526-35. 2004..Unlike other functions of Importin alpha, this membrane-associated activity does not require interaction with NLS proteins... - Cdk11 is a RanGTP-dependent microtubule stabilization factor that regulates spindle assembly rateHideki Yokoyama
European Molecular Biology Laboratory, Heidelberg 69117, Germany
J Cell Biol 180:867-75. 2008..Our results indicate that Cdk11 is responsible for RanGTP-dependent MT stabilization around chromosomes and that this local stabilization is essential for normal rates of spindle assembly and spindle function... - Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebratesCerstin Franz
European Molecular Biology Laboratory, Heidelberg, Germany
EMBO J 24:3519-31. 2005..Time-course experiments indicated that Nup155 is recruited to chromatin at the time of NE sealing, suggesting that nuclear pore complex assembly has to progress to a relatively late stage before NE membrane assembly occurs... - 40LoVe interacts with Vg1RBP/Vera and hnRNP I in binding the Vg1-localization elementKevin Czaplinski
EMBL Gene Expression Programme, Heidelberg, Germany
RNA 12:213-22. 2006..We further demonstrate that titration of VM1 and E2 motif binding activity in vivo surprisingly suggests that the motif binding proteins have differing roles during Vg1LE-dependent mRNA localization... - NuSAP, a mitotic RanGTP target that stabilizes and cross-links microtubulesKatharina Ribbeck
European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
Mol Biol Cell 17:2646-60. 2006..Once activated, NuSAP may aid to maintain spindle integrity by stabilizing and cross-linking microtubules around chromatin... - A role for NuSAP in linking microtubules to mitotic chromosomesKatharina Ribbeck
European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Curr Biol 17:230-6. 2007..We propose that the presence of MT binding agents such as NuSAP, which can be directly immobilized on chromatin, are critical for targeting MT production to vertebrate chromosomes during spindle self-organization... - The conserved Nup107-160 complex is critical for nuclear pore complex assemblyTobias C Walther
EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Cell 113:195-206. 2003..Depletion also prevented association of FG-repeat nucleoporins with chromatin. We propose a stepwise model in which postmitotic NPC assembly initiates on chromatin via early recruitment of the Nup107-160 complex... - The SMN complex is associated with snRNPs throughout their cytoplasmic assembly pathwaySéverine Massenet
Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
Mol Cell Biol 22:6533-41. 2002..Thus, the SMN complex is associated with snRNPs during the entire process of their biogenesis in the cytoplasm and may have multiple functions throughout this process... - The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein importTobias C Walther
Gene Expression Program, EMBL, D-69117 Heidelberg, Germany
J Cell Biol 158:63-77. 2002..We conclude that RanBP2 is the major nucleoporin component of the cytoplasmic filaments of the NPC, and that these filaments do not have an essential role in importin alpha/beta- or transportin-dependent import... - Steady-state nuclear localization of exportin-t involves RanGTP binding and two distinct nuclear pore complex interaction domainsScott Kuersten
Gene Expression Programme, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
Mol Cell Biol 22:5708-20. 2002..We propose that these interactions increase the concentration of tRNA export complexes and of empty Xpo-t in the vicinity of NPCs and thus increase the efficiency of the Xpo-t transport cycle... - Identification and characterization of RED120: a conserved PWI domain protein with links to splicing and 3'-end formationPuri Fortes
Division of Gene Therapy and Hepatology, CIMA, University of Navarra, Pamplona, Spain
FEBS Lett 581:3087-97. 2007..These results suggest that RED120 may function to couple splicing with mRNA 3'-end formation... - The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assemblyMartin Hetzer
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Nat Cell Biol 4:E177-84. 2002..Ran functions in these events as a signal for chromosome position... - Direct membrane protein-DNA interactions required early in nuclear envelope assemblySebastian Ulbert
European Molecular Biology Laboratory, 69117 Heidelberg, Germany
J Cell Biol 173:469-76. 2006..Membrane fractions that are essential for NE formation are shown to bind directly to protein-free DNA, and our data suggest that these interactions are critical for early steps in NE assembly... - HURP is part of a Ran-dependent complex involved in spindle formationMaria D Koffa
EMBL, Heidelberg, Germany
Curr Biol 16:743-54. 2006..CONCLUSIONS: HURP is a new and essential component of the mitotic apparatus. HURP acts as part of a multicomponent complex that affects the growth or stability of spindle MTs and is required for spindle MT organization... - The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cellsJörg Mansfeld
Institute of Biochemistry, ETH Zurich, CH 8093 Zurich, Switzerland
Mol Cell 22:93-103. 2006..Importantly, NDC1 interacts with Nup53 in vitro. This suggests that NDC1 function involves forming a link between the NE membrane and soluble nucleoporins, thereby anchoring the NPC in the membrane... - Ran binds to chromatin by two distinct mechanismsDaniel Bilbao-Cortes
European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Curr Biol 12:1151-6. 2002..We propose that the Ran binding capacity of chromatin contributes to localized spindle and NE assembly... - Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryosPeter Askjaer
European Molecular Biology Laboratory, 69117 Heidelberg, Germany
Mol Biol Cell 13:4355-70. 2002..Finally, we have demonstrated that both IMA-2 and IMB-1, the homologues of vertebrate importin alpha and beta, are essential for both spindle assembly and nuclear formation in early embryos... - Large-scale induced fit recognition of an m(7)GpppG cap analogue by the human nuclear cap-binding complexCatherine Mazza
European Molecular Biology Laboratory, Grenoble Outstation, c o ILL, BP 181, F 38042 Grenoble Cedex 9, France
EMBO J 21:5548-57. 2002..Implications for the transfer of capped mRNAs to eIF4E, required for translation initiation, are discussed... - Quantitative models of nuclear transportAttila Becskei
Department of Physics, Massachusetts Institute of Technology, 13 2009, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
Curr Opin Cell Biol 17:27-34. 2005..Recent evidence suggests that the distinctive kinetics and spatial organization of nuclear transport processes can be used to efficiently propagate signals by new and unexpected pathways... - The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formationWolfram Antonin
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Mol Cell 17:83-92. 2005.... - Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complexCarlo Petosa
European Molecular Biology Laboratory, Grenoble Outstation, B P 181, 38042 Grenoble Cedex 9, France
Mol Cell 16:761-75. 2004..Our findings indicate that a single architectural and mechanistic framework can explain the divergent effects of RanGTP on substrate binding by many import and export receptors... - Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cellsOliver J Gruss
EMBL, Meyerhofstrasse 1, 69 117 Heidelberg, Germany
Nat Cell Biol 4:871-9. 2002..This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells... - Co-crystallization of the human nuclear cap-binding complex with a m7GpppG cap analogue using protein engineeringCatherine Mazza
European Molecular Biology Laboratory, Grenoble Outstation, c/o ILL, BP 181, F-38042 Grenoble Cedex 9, France
Acta Crystallogr D Biol Crystallogr 58:2194-7. 2002..3 A resolution. In both forms, strong extra electron density is observed for the cap analogue and for the N- and C-terminal extensions of CBP20 which was absent or disordered in all previous structures... - Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivoVincent Galy
European Molecular Biology Laboratory, 69117 Heidelberg, Germany
Mol Biol Cell 14:5104-15. 2003..Thus, the contribution to NPC structure of Nup93 and Nup205 is essential for establishment of normal NPC function and for cell viability... - RanGTP mediates nuclear pore complex assemblyTobias C Walther
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Nature 424:689-94. 2003..The data demonstrate that RanGTP triggers distinct steps of NPC assembly, and suggest a mechanism for the spatial restriction of NPC assembly to the surface of chromatin... - An ATP-dependent activity that releases RanGDP from NTF2Masami Yamada
European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
J Biol Chem 279:36228-34. 2004..A protein phosphatase inhibitor mixture stimulates the RanGDF activity, suggesting the activity is regulated by phosphorylation. We propose that the ATP-dependent NTF2 releasing factor may have a role in the RanGDP/GTP cycle...