D Marsh

Summary

Country: Germany

Publications

  1. pmc Lipid-binding proteins: structure of the phospholipid ligands
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, 37070 Gottingen, Germany
    Protein Sci 12:2109-17. 2003
  2. ncbi request reprint Elastic curvature constants of lipid monolayers and bilayers
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Dept Spektroskopie, 37070 Gottingen, Germany
    Chem Phys Lipids 144:146-59. 2006
  3. doi request reprint Interaction of a peptide derived from glycoprotein gp36 of feline immunodeficiency virus and its lipoylated analogue with phospholipid membranes
    Gerardino D'Errico
    Dipartimento di Chimica, Universita di Napoli Federico II, Napoli, Italy, Dipartimento di Scienze Farmaceutiche, Universita di Salerno, Fisciano, Italy
    Biochemistry 47:5317-27. 2008
  4. doi request reprint Liquid-ordered phases induced by cholesterol: a compendium of binary phase diagrams
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie und Photochemische Kinetik, 37070 Gottingen, Germany
    Biochim Biophys Acta 1798:688-99. 2010
  5. ncbi request reprint Anisotropic motion effects in CW non-linear EPR spectra: relaxation enhancement of lipid spin labels
    V A Livshits
    Centre of Photochemistry, Russian Academy of Sciences, 117427, Moscow, Russian Federation
    J Magn Reson 162:429-42. 2003
  6. pmc Phase diagram of ternary cholesterol/palmitoylsphingomyelin/palmitoyloleoyl-phosphatidylcholine mixtures: spin-label EPR study of lipid-raft formation
    Irina V Ionova
    Centre of Photochemistry, Russian Academy of Sciences, Moscow, Russia
    Biophys J 102:1856-65. 2012
  7. doi request reprint Intermediate dipolar distances from spin labels
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, 37070 Gottingen, Germany Electronic address
    J Magn Reson 238:77-81. 2014
  8. doi request reprint Librational fluctuations in protein glasses
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Biochim Biophys Acta 1834:1591-5. 2013
  9. doi request reprint Orientation and conformation of lipids in crystals of transmembrane proteins
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, 37070, Gottingen, Germany
    Eur Biophys J 42:119-46. 2013
  10. pmc Thermodynamics of phospholipid self-assembly
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Biophys J 102:1079-87. 2012

Collaborators

Detail Information

Publications109 found, 100 shown here

  1. pmc Lipid-binding proteins: structure of the phospholipid ligands
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, 37070 Gottingen, Germany
    Protein Sci 12:2109-17. 2003
    ..Stereochemical violations appear for some of the ester carboxyl groups of the protein-bound phospholipids in the PDB, and two glycerol backbones have the incorrect enantiomeric configuration...
  2. ncbi request reprint Elastic curvature constants of lipid monolayers and bilayers
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Dept Spektroskopie, 37070 Gottingen, Germany
    Chem Phys Lipids 144:146-59. 2006
    ..Consideration is given to the relation between the bending moduli of bilayers and the constituent monolayer leaflets. Useful mathematical relations involving the bending moduli and spontaneous curvature are summarized...
  3. doi request reprint Interaction of a peptide derived from glycoprotein gp36 of feline immunodeficiency virus and its lipoylated analogue with phospholipid membranes
    Gerardino D'Errico
    Dipartimento di Chimica, Universita di Napoli Federico II, Napoli, Italy, Dipartimento di Scienze Farmaceutiche, Universita di Salerno, Fisciano, Italy
    Biochemistry 47:5317-27. 2008
    ....
  4. doi request reprint Liquid-ordered phases induced by cholesterol: a compendium of binary phase diagrams
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie und Photochemische Kinetik, 37070 Gottingen, Germany
    Biochim Biophys Acta 1798:688-99. 2010
    ..Intercomparison of the various data sets allows critical assessment of which phase boundaries are rigorously established from direct evidence for phase coexistence...
  5. ncbi request reprint Anisotropic motion effects in CW non-linear EPR spectra: relaxation enhancement of lipid spin labels
    V A Livshits
    Centre of Photochemistry, Russian Academy of Sciences, 117427, Moscow, Russian Federation
    J Magn Reson 162:429-42. 2003
    ..Experimental examples are given for spin-labelled lipids in membranes...
  6. pmc Phase diagram of ternary cholesterol/palmitoylsphingomyelin/palmitoyloleoyl-phosphatidylcholine mixtures: spin-label EPR study of lipid-raft formation
    Irina V Ionova
    Centre of Photochemistry, Russian Academy of Sciences, Moscow, Russia
    Biophys J 102:1856-65. 2012
    ..These studies define the compositions of raft-like L(o) phases for a minimal realistic biological lipid mixture...
  7. doi request reprint Intermediate dipolar distances from spin labels
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, 37070 Gottingen, Germany Electronic address
    J Magn Reson 238:77-81. 2014
    ..Evaluations of reff,n for Gaussian distributions in r12 yield calibrations to determine 〈r12〉 for a given distribution width, σ(r12)...
  8. doi request reprint Librational fluctuations in protein glasses
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Biochim Biophys Acta 1834:1591-5. 2013
    ..Similar results are found for librational fluctuations of membranous Na,K-ATPase spin-labelled either on superficial SH groups or on those essential to activity. ..
  9. doi request reprint Orientation and conformation of lipids in crystals of transmembrane proteins
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, 37070, Gottingen, Germany
    Eur Biophys J 42:119-46. 2013
    ..Some lipids have the incorrect enantiomeric configuration of the glycerol backbone, and many of the branched methyl groups in the phytanyl chains associated with bacteriorhodopsin have the incorrect S configuration...
  10. pmc Thermodynamics of phospholipid self-assembly
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Biophys J 102:1079-87. 2012
    ..These measurements help define the thermodynamic effects of anionic lipids on biomembrane stability...
  11. pmc Water adsorption isotherms of lipids
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Biophys J 101:2704-12. 2011
    ..The relationship of the latter approach to measurements of hydration forces between lipid bilayers is discussed...
  12. doi request reprint Bilayer dimensions and hydration of glycolipids
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, 37070 Gottingen, Germany
    Chem Phys Lipids 165:23-31. 2012
    ..A point of current uncertainty is whether the chains in the gel phases of diacyl glycoglycerolipids are appreciably tilted, unlike their dialkyl counterparts...
  13. doi request reprint Lateral order in gel, subgel and crystalline phases of lipid membranes: wide-angle X-ray scattering
    Derek Marsh
    Max Planck Insitut für biophysikalische Chemie, 37077 Gottingen, Germany
    Chem Phys Lipids 165:59-76. 2012
    ..These parameters determine many of the properties of the ordered lipid phases and, together with the chain tilt, specify the area occupied by the lipid head groups at the surface of the bilayer...
  14. pmc Membrane elastic fluctuations and the insertion and tilt of beta-barrel proteins
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, Gottingen, Germany
    Biophys J 91:227-32. 2006
    ..L. Pocanschi, J. H. Kleinschmidt, and D. Marsh. 2005. Biochemistry. 44:3515-3523) is also considered. A contribution to the orientational order parameter that scales as the inverse second power of the membrane thickness is predicted...
  15. ncbi request reprint Lipid conformation in crystalline bilayers and in crystals of transmembrane proteins
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, Gottingen, Germany
    Chem Phys Lipids 141:48-65. 2006
    ....
  16. ncbi request reprint Comment on interpretation of mechanochemical properties of lipid bilayer vesicles from the equation of state or pressure-area measurement of the monolayer at the air-water or oil-water interface
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, Am Fassberg 11, 37077 Gottingen, Germany
    Langmuir 22:2916-9; discussion 2920-2. 2006
  17. pmc Oxygen profiles in membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, 37070 Gottingen, Germany
    Biophys J 90:L49-51. 2006
    ....
  18. ncbi request reprint High-field spin-label EPR of lipid membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, 37070 Gottingen, Germany
    Magn Reson Chem 43:S20-5. 2005
    ..These results are obtained from phosphatidylcholine spin probes with the nitroxide systematically stepped down the sn-2 chain, in fully hydrated bilayer membranes of dimyristoyl phosphatidylcholine (DMPC)-containing cholesterol...
  19. ncbi request reprint The protein-lipid interface: perspectives from magnetic resonance and crystal structures
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biochim Biophys Acta 1666:118-41. 2004
    ..Finally, the interactions between integral proteins and peripheral or lipid-linked proteins, as reflected by the lipid-protein interactions in double reconstitutions, are considered...
  20. pmc Electron spin resonance in membrane research: protein-lipid interactions from challenging beginnings to state of the art
    Derek Marsh
    Abteilung Spektroskopie, Max Planck Institut fur biophysikalische Chemie, 37070 Gottingen, Germany
    Eur Biophys J 39:513-25. 2010
    ..The sensitivity of non-linear EPR to low frequencies of spin exchange also allows the location of spin-labelled membrane protein residues relative to those of spin-labelled lipids, in double-labelling experiments...
  21. ncbi request reprint Infrared dichroism of isotope-edited alpha-helices and beta-sheets
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, 37070 Gottingen, Germany
    J Mol Biol 338:353-67. 2004
    ..The orientation of the individual transition moments in a beta-sheet that is essential for this analysis is derived for the different amide bands...
  22. ncbi request reprint Lipid interactions with transmembrane proteins
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Cell Mol Life Sci 60:1575-80. 2003
    ..Chain C-C dihedral angles are, however, not restricted to the energetically allowable trans and gauche rotamers.This indicates that the chains of a given lipid do not have a unique configuration in protein crystals...
  23. ncbi request reprint Lipid membranes with grafted polymers: physicochemical aspects
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, Am Fassberg 11, D 37077 Gottingen, Germany
    Biochim Biophys Acta 1615:33-59. 2003
    ..Topics covered are: mushroom-brush transitions, membrane expansion and elasticity, bilayer-micelle transitions, membrane-membrane interactions and protein-membrane interactions...
  24. pmc TOAC spin labels in the backbone of alamethicin: EPR studies in lipid membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, Gottingen, Germany
    Biophys J 92:473-81. 2007
    ..Thus, alamethicin does not form large stable aggregates in fluid membranes, and ionic conductance must arise from transient or voltage-induced associations...
  25. pmc Lipid chain-length dependence for incorporation of alamethicin in membranes: electron paramagnetic resonance studies on TOAC-spin labeled analogs
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, 37070 Gottingen, Germany
    Biophys J 92:4002-11. 2007
    ..Alamethicin differs in its mode of incorporation from other helical peptides for which transmembrane orientation has been determined as a function of lipid chain length...
  26. doi request reprint Pivotal surfaces in inverse hexagonal and cubic phases of phospholipids and glycolipids
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Chem Phys Lipids 164:177-83. 2011
    ..The area per lipid at the pivotal plane is similar in size to lipid cross-sectional areas found in the fluid lamellar phase (L(α)) of lipid bilayers...
  27. doi request reprint Molecular volumes of phospholipids and glycolipids in membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Chem Phys Lipids 163:667-77. 2010
    ..Lipid volumes are determining parameters for lipid dynamics, membrane partitioning and permeation of solutes, and are essential quantities for the structural analysis of lipid membranes...
  28. doi request reprint Structural and thermodynamic determinants of chain-melting transition temperatures for phospholipid and glycolipids membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie und Photochemische Kinetik, 37070 Gottingen, Germany
    Biochim Biophys Acta 1798:40-51. 2010
    ..This type of information is essential for biophysical approaches to functional lipidomics...
  29. doi request reprint Cholesterol-induced fluid membrane domains: a compendium of lipid-raft ternary phase diagrams
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie und Photochemische Kinetik, 37070 Gottingen, Germany
    Biochim Biophys Acta 1788:2114-23. 2009
    ..It is then possible to appreciate the common features and examine critically the discrepancies and hence what direct biophysical evidence there is that supports the raft concept...
  30. pmc Reaction fields in the environment of fluorescent probes: polarity profiles in membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, Gottingen, Germany
    Biophys J 96:2549-58. 2009
    ..An important application is calibration of the transmembrane polarity profile recorded by fluorescent probes in terms of the high-resolution profile obtained from site-specifically spin-labeled lipid chains...
  31. doi request reprint Orientation and peptide-lipid interactions of alamethicin incorporated in phospholipid membranes: polarized infrared and spin-label EPR spectroscopy
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biochemistry 48:729-37. 2009
    ....
  32. doi request reprint Electron spin resonance in membrane research: protein-lipid interactions
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, Am Fassberg 11, 37070 Gottingen, Germany
    Methods 46:83-96. 2008
    ..Instrumental requirements are considered, and also sample handling, spin-labelling techniques and the synthesis of spin-labelled lipids...
  33. doi request reprint Protein modulation of lipids, and vice-versa, in membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, Gottingen, Germany
    Biochim Biophys Acta 1778:1545-75. 2008
    ....
  34. pmc Energetics of hydrophobic matching in lipid-protein interactions
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37077 Gottingen, Germany
    Biophys J 94:3996-4013. 2008
    ....
  35. ncbi request reprint Polarity dependence of EPR parameters for TOAC and MTSSL spin labels: correlation with DOXYL spin labels for membrane studies
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37077 Gottingen, Germany
    J Magn Reson 190:211-21. 2008
    ..A similar calibration procedure is also applied to a(o)(N) and g(o) for the pyrroline methanethiosulphonate nitroxide (MTSSL) that is used in site-directed spin-labelling studies of membrane proteins...
  36. pmc Lateral pressure profile, spontaneous curvature frustration, and the incorporation and conformation of proteins in membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, Gottingen, Germany
    Biophys J 93:3884-99. 2007
    ..Changes in chemical potential approximately 12 kJ mol(-1) can be estimated for radial changes of 1 A in a protein of diameter 40 A...
  37. ncbi request reprint Reaction fields and solvent dependence of the EPR parameters of nitroxides: the microenvironment of spin labels
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, 37077 Gottingen, Germany
    J Magn Reson 190:60-7. 2008
    ..g., in membranes) between different nitroxide spin labels (e.g., of the TEMPO and DOXYL variety)...
  38. ncbi request reprint Application of electron spin resonance for investigating peptide-lipid interactions, and correlation with thermodynamics
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biochem Soc Trans 29:582-9. 2001
    ..Comparison with thermodynamic predictions for membrane insertion provides a useful adjunct to the spin-label method...
  39. pmc Elastic constants of polymer-grafted lipid membranes
    D Marsh
    Abt Spektroskopie, Max Planck Institut fur biophysikalische Chemie, 37070 Gottingen, Germany
    Biophys J 81:2154-62. 2001
    ..The polymer brush contribution to spontaneous curvature scales quadratically with the polymer length, and at least quadratically with the mole fraction of polymer lipid...
  40. ncbi request reprint Structure, dynamics and composition of the lipid-protein interface. Perspectives from spin-labelling
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, D 37070 Gottingen, Germany
    Biochim Biophys Acta 1376:267-96. 1998
    ..Comparison with the results obtained from integral proteins helps to define the extent of membrane penetration and degree of transmembrane crossing that are relevant to protein translocation mechanisms...
  41. ncbi request reprint Membrane water-penetration profiles from spin labels
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroscopie, 37070 Gottingen, Germany
    Eur Biophys J 31:559-62. 2002
    ..Water penetration is appreciable, depends on chain unsaturation, and is strongly affected by cholesterol...
  42. pmc Thermodynamic analysis of chain-melting transition temperatures for monounsaturated phospholipid membranes: dependence on cis-monoenoic double bond position
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, D 37070 Gottingen, Germany
    Biophys J 77:953-63. 1999
    ....
  43. pmc Nonaxiality in infrared dichroic ratios of polytopic transmembrane proteins
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, D 37070 Gottingen, Germany
    Biophys J 75:354-8. 1998
    ..The nonaxial contributions to the dichroic ratios of beta-barrel oligomers are also derived. Estimates are given of the likely size of the nonaxial contributions for the different alpha-helical and beta-sheet systems...
  44. pmc Polarity and permeation profiles in lipid membranes
    D Marsh
    Abteilung Spektroskopie, Max Planck Institut fur biophysikalische Chemie, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 98:7777-82. 2001
    ....
  45. pmc Quantitation of secondary structure in ATR infrared spectroscopy
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, D 37070 Gottingen, Germany
    Biophys J 77:2630-7. 1999
    ..With unpolarized radiation, this correction is not possible. Similar considerations apply to the dichroic ratios of multicomponent bands, which are also treated...
  46. ncbi request reprint High-field electron spin resonance of spin labels in membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, Göttingen 37070, Germany
    Chem Phys Lipids 116:93-114. 2002
    ..These areas are treated in some depth, along with a detailed consideration of recent developments in the investigation of transmembrane polarity profiles...
  47. pmc Renormalization of the tension and area expansion modulus in fluid membranes
    D Marsh
    Abteilung Spektroskopie, Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Biophys J 73:865-9. 1997
    ..Rev. Lett. 64:2094-2097, 1990), and suggests conditions for molecular dynamics simulations with membrane patches of limited size that might best represent the properties of macroscopic vesicles...
  48. ncbi request reprint Derivatised lipids in membranes. Physico-chemical aspects of N-biotinyl phosphatidylethanolamines, N-acyl phosphatidylethanolamines and N-acyl ethanolamines
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, Gottingen, Germany
    Chem Phys Lipids 105:43-69. 2000
    ..All of these properties are relevant to the role of these rather unusual lipids in membranes...
  49. ncbi request reprint Specific spin labelling of the sugar-H(+) symporter, GalP, in cell membranes of Escherichia coli: site mobility and overall rotational diffusion of the protein
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Biochim Biophys Acta 1510:464-73. 2001
    ..The rate of rotation about the membrane normal at 37 degrees C is consistent with predictions for a 12-transmembrane helix assembly that is less than closely packed...
  50. pmc Dichroic ratios in polarized Fourier transform infrared for nonaxial symmetry of beta-sheet structures
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, Gottingen, Germany
    Biophys J 72:2710-8. 1997
    ..The results are applied to previous ATR and polarized transmission FTIR measurements on a potassium channel-associated peptide, the Escherichia coli outer membrane protein OmpA, and the E. coli OmpF porin protein in oriented membranes...
  51. ncbi request reprint Interaction of membrane-spanning proteins with peripheral and lipid-anchored membrane proteins: perspectives from protein-lipid interactions (Review)
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Mol Membr Biol 19:247-55. 2002
    ..Both the former and the cytochrome c/cytochrome oxidase couple evidence a propagation of lipid perturbation out from the intramembrane protein interface that could be a basis for formation of microdomains...
  52. ncbi request reprint Infrared dichroism of twisted beta-sheet barrels. The structure of E. coli outer membrane proteins
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, D 37070 Gottingen, Germany
    J Mol Biol 297:803-8. 2000
    ..Hydrophobic matching of the 2.6 nm transmembrane stretch partly determines the shear number of the OmpA beta-barrel...
  53. pmc Infrared dichroism from the X-ray structure of bacteriorhodopsin
    D Marsh
    Abteilung Spektroskopie, Max Planck Institut fur biophysikalische Chemie, 37070 Gottingen, Germany
    Biophys J 80:305-12. 2001
    ..These estimates are close to those found for model alpha-helical polypeptides, indicating that side-chain heterogeneity and slight helix imperfections are unlikely to affect the reliability of infrared measurements of helix orientations...
  54. pmc Orientation of the infrared transition moments for an alpha-helix
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, D 37070 Gottingen, and Institut fur Polymerforschung, D 01005 Dresden, Germany
    Biophys J 78:2499-510. 2000
    ..The measurements are discussed both in the context of the somewhat divergent older determinations, and in relation to the helix geometry and results on model amide compounds, to resolve current uncertainties in the literature...
  55. ncbi request reprint Relaxation time determinations by progressive saturation EPR: effects of molecular motion and Zeeman modulation for spin labels
    V A Livshits
    Abteilung Spektroskopie, , , D 37070, Germany
    J Magn Reson 133:79-91. 1998
    ....
  56. pmc Binding of peripheral proteins to mixed lipid membranes: effect of lipid demixing upon binding
    T Heimburg
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, D 37070 Gottingen, Germany
    Biophys J 76:2575-86. 1999
    ..Using the value of Kr obtained it is possible to derive the average in-plane lipid distribution and the enhancement in protein binding induced by lipid redistribution using the statistical thermodynamic theory...
  57. doi request reprint Incorporation of outer membrane protein OmpG in lipid membranes: protein-lipid interactions and beta-barrel orientation
    V Anbazhagan
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biochemistry 47:6189-98. 2008
    ..These systematic features of the integration of monomeric beta-barrel proteins in lipid membranes could be useful for characterizing outer membrane proteins of unknown structure...
  58. ncbi request reprint Specific surface association of avidin with N-biotinylphosphatidylethanolamine membrane assemblies: effect on lipid phase behavior and acyl-chain dynamics
    M J Swamy
    Abteilung Spektroskopie, , , Germany
    Biochemistry 40:14869-77. 2001
    ..These effects may be important for both lateral and transbilayer communication in the membrane...
  59. ncbi request reprint Interactions of angiotensin II non-peptide AT(1) antagonist losartan with phospholipid membranes studied by combined use of differential scanning calorimetry and electron spin resonance spectroscopy
    E Theodoropoulou
    Abteilung Spektroskopie und Photochemische Kinetik, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077, Gottingen, Germany
    Biochim Biophys Acta 1461:135-46. 1999
    ..As a conclusion, our study has shown that Losartan interacts with phospholipid membranes by affecting both their thermotropic behavior and molecular mobility...
  60. ncbi request reprint Interaction of cholesterol with sphingomyelin in mixed membranes containing phosphatidylcholine, studied by spin-label ESR and IR spectroscopies. A possible stabilization of gel-phase sphingolipid domains by cholesterol
    M P Veiga
    , Abteilung Spektroskopie, , Germany
    Biochemistry 40:2614-22. 2001
    ..This result provides some support for the suggestion that transbilayer interdigitation induced by cholesterol stabilizes the coexistence of gel-phase and "liquid-ordered" domains in membranes containing sphingolipids...
  61. pmc Intrinsic curvature in normal and inverted lipid structures and in membranes
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, Gottingen
    Biophys J 70:2248-55. 1996
    ..08) is obtained for DOPC. The energetics of the intrinsic curvature and lamellar-nonlamellar transitions are also discussed within the framework of the model...
  62. ncbi request reprint Interactions at the membrane surface studied by spin label ESR spectroscopy
    D Marsh
    Max Planck Institut fur biophysikalische Chemie, Karl Friedrich Bonhoeffer Institut, Gottingen, Germany
    Biosci Rep 19:253-9. 1999
    ..A description of these illustrates both the capabilities of this biophysical methodology and the functional/technological implications of these interactions and dynamic/thermodynamic processes for cell membranes and their surfaces...
  63. ncbi request reprint Association of alpha-synuclein and mutants with lipid membranes: spin-label ESR and polarized IR
    Muthu Ramakrishnan
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biochemistry 45:3386-95. 2006
    ..The beta-sheet secondary-structural elements of the wild-type and truncated proteins are partially oriented on the surface of membranes with which they interact...
  64. ncbi request reprint Orientation of beta-barrel proteins OmpA and FhuA in lipid membranes. Chain length dependence from infrared dichroism
    Muthu Ramakrishnan
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biochemistry 44:3515-23. 2005
    ..The orientational ordering of the beta-barrels and their assembly in the membrane are discussed in terms of hydrophobic matching with the lipid chains...
  65. ncbi request reprint Lipid-protein interactions and effect of local anesthetics in acetylcholine receptor-rich membranes from Torpedo marmorata electric organ
    S B Mantipragada
    Abteilung Spektroskopie, Max Planck Institut fur biophysikalische Chemie, 37077 Gottingen, Germany
    Biochemistry 42:9167-75. 2003
    ..These displacements represent an intrinsic difference in affinity of the local anesthetics for the lipid-protein interface because the membrane partition coefficients are in the order benzocaine >> tetracaine approximately procaine...
  66. pmc Tilt, twist, and coiling in beta-barrel membrane proteins: relation to infrared dichroism
    T Pali
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, 37070 Gottingen, Germany
    Biophys J 80:2789-97. 2001
    ..The strand tilt obtained from the x-ray coordinates, or from the reparameterised model, can be combined with infrared dichroism measurements to obtain information on the orientation of the beta-barrel assembly in the membrane...
  67. doi request reprint Protein-lipid interactions with Fusobacterium nucleatum major outer membrane protein FomA: spin-label EPR and polarized infrared spectroscopy
    V Anbazhagan
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biochemistry 47:8414-23. 2008
    ..Comparison with similar data from the 8- to 22-stranded E. coli outer membrane proteins, OmpA, OmpG, and FhuA, supports the above conclusions...
  68. pmc Oxygen permeation profile in lipid membranes: comparison with transmembrane polarity profile
    Boris G Dzikovski
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biophys J 85:1005-12. 2003
    ..At the protein interface of the KcsA ion channel, the oxygen profile is more diffuse than that in fluid lipid bilayers...
  69. pmc Differential scanning calorimetry of thermotropic phase transitions in vitaminylated lipids: aqueous dispersions of N-biotinyl phosphatidylethanolamines
    M J Swamy
    , Abteilung Spektroskopie, , Germany
    Biophys J 66:31-9. 1994
    ..Additionally, the lipids with shorterchainlengths exhibit metastability in the absence of salt, converting from a micellar solution to a lamellar gel phase only after incubation at low temperature with freeze-thaw cycling...
  70. pmc Lateral ordering of lipid chains in cholesterol-containing membranes: high-field spin-label EPR
    Dieter Kurad
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, 37070 Gottingen, Germany
    Biophys J 86:264-71. 2004
    ..The nonaxial lipid ordering may be related to the formation of in-plane lipid domains in membranes containing cholesterol and saturated lipids...
  71. pmc Stoichiometry of lipid interactions with transmembrane proteins--Deduced from the 3D structures
    Tibor Pali
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Protein Sci 15:1153-61. 2006
    ..Deviations from the geometrical first shell, in the beta-barrel case, are for the smaller protein with a highly curved barrel...
  72. ncbi request reprint Effect of angiotensin II non-peptide AT(1) antagonist losartan on phosphatidylethanolamine membranes
    E Theodoropoulou
    Abteilung Spektroskopie und Photochemische Kinetik, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077, Gottingen, Germany
    Biochim Biophys Acta 1509:346-60. 2000
    ..Furthermore, its interactions with phosphatidylethanolamine membranes are more superficial than with phosphatidylcholine bilayers...
  73. ncbi request reprint Application of the out-of-phase absorption mode to separating overlapping EPR signals with different T1 values
    V A Livshits
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37077 Gottingen, Germany
    J Magn Reson 175:317-29. 2005
    ..It is concluded that optimal resolution in V1'-EPR spectroscopy is obtained at relatively low Zeeman modulation frequencies (20-30 kHz) and moderate saturation (H1 approximately 0.2-0.3 G)...
  74. ncbi request reprint Multifrequency simulations of the EPR spectra of lipid spin labels in membranes
    V A Livshits
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37077 Gottingen, Germany
    J Magn Reson 180:63-71. 2006
    ..Inclusion of spectra at 34GHz greatly improves precision in determining the D(R//) element of the slow diffusion tensor in these systems...
  75. doi request reprint Incorporation of transmembrane peptides from the vacuolar H(+)-ATPase in phospholipid membranes: spin-label electron paramagnetic resonance and polarized infrared spectroscopy
    Zoltan Kota
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biochemistry 47:3937-49. 2008
    ..Comparison of the peptide behavior in lipid bilayers is made with membranous subunit c assemblies of the 16-kDa proteolipid from Nephrops norvegicus, which can substitute functionally for Vma3p in S. cerevisiae...
  76. ncbi request reprint Association of spin-labeled lipids with beta-barrel proteins from the outer membrane of Escherichia coli
    Muthu Ramakrishnan
    Abteilung Spektroskopie, Max Planck Institut fur biophysikalische Chemie, 37077 Gottingen, Germany
    Biochemistry 43:11630-6. 2004
    ....
  77. ncbi request reprint Alpha-synuclein association with phosphatidylglycerol probed by lipid spin labels
    Muthu Ramakrishnan
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biochemistry 42:12919-26. 2003
    ..Accordingly, membrane-bound alpha-synuclein associates at the interfacial region of the bilayer where it may favor a local concentration of certain phospholipids...
  78. ncbi request reprint Mechanism of relaxation enhancement of spin labels in membranes by paramagnetic ion salts: dependence on 3d and 4f ions and on the anions
    V A Livshits
    , Abteilung Spektroskopie, , Germany
    J Magn Reson 148:221-37. 2001
    ..On this basis, different paramagnetic agents may be chosen to optimize sensitivity to different structurally correlated interactions. These results therefore will aid further spin-label EPR studies in structural biology...
  79. pmc Lipid membrane polarity profiles by high-field EPR
    Dieter Kurad
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, 37070 Gottingen, Germany
    Biophys J 85:1025-33. 2003
    ..In the transition region between high- and low-polarity regions (n approximately 8), the g(xx)-feature consists of two components characteristic of coexisting hydrated and nonhydrated states...
  80. ncbi request reprint Orientation of TOAC amino-acid spin labels in alpha-helices and beta-strands
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    J Magn Reson 180:305-10. 2006
    ..The spin-label z-axes of the two mirror-image TOAC twist-boat conformers are inclined at 13+/-2 degrees and 65+/-3 degrees , respectively, to the alpha-helix axis, or at 25+/-3 degrees and 32+/-3 degrees to the beta-strand axis...
  81. ncbi request reprint Solvent dependence of the rotational diffusion of TOAC-spin-labeled alamethicin
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, Gottingen, Germany
    Chem Biodivers 4:1269-74. 2007
    ..Even in HxOH, the (fivefold) increase in correlation time was no greater than the increase in viscosity. We conclude that TOAC-labeled alamethicins remain monomeric in these solvents of relatively high polarity...
  82. ncbi request reprint Lipid-protein interactions with cardiac phospholamban studied by spin-label electron spin resonance
    Ashish Arora
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37077 Gottingen, Germany
    Biochemistry 42:5151-8. 2003
    ..A similar pattern of lipid selectivity is obtained in DMPG, but the absolute selectivities are reduced considerably. The results are discussed in terms of the integration of the regulatory species in the lipid membrane...
  83. ncbi request reprint Interaction of inhibitors of the vacuolar H(+)-ATPase with the transmembrane Vo-sector
    Tibor Pali
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, 37070 Gottingen, Germany
    Biochemistry 43:12297-305. 2004
    ..Inhibition of proton transport may also involve immobilization of the overall rotation of the proteolipid subunit assembly...
  84. pmc Structural characterization of copper(II) binding to alpha-synuclein: Insights into the bioinorganic chemistry of Parkinson's disease
    Rodolfo M Rasia
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 102:4294-9. 2005
    ..The metallobiology of Cu(II) in Parkinson's disease is discussed by a comparative analysis with other Cu(II)-binding proteins involved in neurodegenerative disorders...
  85. ncbi request reprint A divalent-ion binding site on the 16-kDa proton channel from Nephrops norvegicus--revealed by EPR spectroscopy
    Tibor Pali
    Max Planck Institut fur biophysikalische Chemie, Abteilung Spektroskopie, 37070 Gottingen, Germany
    Biochim Biophys Acta 1758:206-12. 2006
    ....
  86. ncbi request reprint Spin-labelled vacuolar-ATPase inhibitors in lipid membranes
    Neil Dixon
    Department of Chemistry, University of Leeds, Leeds LS2 9JT, UK
    Biochim Biophys Acta 1665:177-83. 2004
    ..These two spin-labelled derivatives provide complementary EPR probes of the lipid environment (INDOL6), and of ligand-protein interactions (INDOL5), for this class of V-ATPase inhibitor...
  87. ncbi request reprint New biophysical probes for structure-activity analyses of vacuolar-H+ -ATPase enzymes
    Neil Dixon
    Department of Chemistry, Woodhouse Lane, Leeds, UK LS2 9JT
    Org Biomol Chem 1:4361-3. 2003
    ..New EPR spin labelled and photoactivatable molecules have been designed to probe specifically the vacuolar-H(+)-ATPase...
  88. pmc Backbone dynamics of alamethicin bound to lipid membranes: spin-echo electron paramagnetic resonance of TOAC-spin labels
    Rosa Bartucci
    Dipartimento di Fisica, Universita della Calabria, 87036 Arcavacata di Rende CS, Italy
    Biophys J 94:2698-705. 2008
    ..Associated fluctuations in polar fields from the peptide could facilitate ion permeation...
  89. ncbi request reprint Domain formation in sphingomyelin/cholesterol mixed membranes studied by spin-label electron spin resonance spectroscopy
    M Isabel Collado
    Departamento de Bioquimica, Universidad del Pais Vasco, Apartado 644, E 48080 Bilbao, Spain
    Biochemistry 44:4911-8. 2005
    ....
  90. pmc Intramembrane polarity by electron spin echo spectroscopy of labeled lipids
    Rosa Bartucci
    Dipartimento di Fisica and Unità INFM, Universita della Calabria, I 87036 Arcavacata di Rende CS, Italy
    Biophys J 84:1025-30. 2003
    ..These results are in good agreement with more indirect measurements of the transmembrane polarity profile that are based on the (14)N-hyperfine splittings in the conventional continuous-wave electron spin resonance spectrum...
  91. pmc Librational motion of spin-labeled lipids in high-cholesterol containing membranes from echo-detected EPR spectra
    Denis A Erilov
    Dipartimento di Fisica and Unità Instituto Nazionale per la Fisica della Materia, Universita della Calabria, Arcavacata di Rende CS, Italy
    Biophys J 87:3873-81. 2004
    ..Such librational motions are important for glass-like states and are generally relevant to high lipid packing densities, e.g., in cholesterol-containing raft domains and condensed complexes...
  92. ncbi request reprint Incorporation of the V-ATPase inhibitors concanamycin and indole pentadiene in lipid membranes. Spin-label EPR studies
    Tibor Pali
    Department of Chemistry, University of Leeds, Leeds LS2 9JT, UK
    Biochim Biophys Acta 1663:14-8. 2004
    ..Therefore, the common mode of addition in V-ATPase inhibition assays ensures incorporation of concanamycin into the lipid bilayer milieu, which provides an efficient channel of access to the transmembrane domains of the V-ATPase...
  93. pmc Scaling and mean-field theories applied to polymer brushes
    Derek Marsh
    Biophys J 86:2630-3. 2004
  94. pmc Orientation and lipid-peptide interactions of gramicidin A in lipid membranes: polarized attenuated total reflection infrared spectroscopy and spin-label electron spin resonance
    Zoltan Kota
    Institute of Biophysics, Biological Research Centre, Szeged, Hungary
    Biophys J 86:1521-31. 2004
    ..These results are discussed in terms of the integration of gramicidin A channels in lipid bilayers, and of the interactions of lipids with integral membrane proteins...
  95. pmc The N-terminal segment of pulmonary surfactant lipopeptide SP-C has intrinsic propensity to interact with and perturb phospholipid bilayers
    Ines Plasencia
    Departamento de Bioquimica y Biologia Molecular I, Facultad de Biologia, Universidad Complutense, 28040 Madrid, Spain
    Biochem J 377:183-93. 2004
    ....
  96. ncbi request reprint Lipid-protein interactions in Escherichia coli membranes over-expressing the sugar-H(+) symporter, GalP EPR of spin-labelled lipids
    Anne Hubert
    School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    Biochim Biophys Acta 1611:243-8. 2003
    ..At the pH of the transport assay, there is relatively little selectivity between the different phospholipids tested. Only stearic acid displays a stronger preferential interaction with this protein...
  97. ncbi request reprint Chain dynamics in the low-temperature phases of lipid membranes by electron spin-echo spectroscopy
    Rosa Bartucci
    Dipartimento di Fisica, Universita della Calabria, I 87036 Arcavacata di Rende CS, Italy
    J Magn Reson 162:371-9. 2003
    ..Cholesterol has a large effect on torsional oscillations about the chain long axis. Small-amplitude chain motions in the low-temperature phases may be important for cryopreservation of membranes...
  98. ncbi request reprint Shifts in chain-melting transition temperature of liposomal membranes by polymer-grafted lipids
    Manuela Pantusa
    Dipartimento di Fisica and Unità INFM, Universita della Calabria, Ponte P Bucci, Cubo 31 C, I 87036, Arcavacata di Rende CS, Italy
    Biochim Biophys Acta 1614:165-70. 2003
    ..1-0.2 degrees C) in reasonable accord with theoretical estimates of the lateral pressure exerted by the polymer brush. Shifts of this size are significant to the design of liposomes for controlled release of contents by mild hyperthermia...
  99. ncbi request reprint Interaction of human serum albumin with membranes containing polymer-grafted lipids: spin-label ESR studies in the mushroom and brush regimes
    Rosa Bartucci
    Lab di Biofisica Molecolare, Dipartimento di Fisica and Unità INFM, Universita della Calabria, Ponte P Bucci, Cubo 31 C, I 87036 Arcavacata di Rende CS, Italy
    Biochim Biophys Acta 1564:237-42. 2002
    ..These results are in agreement with theoretical estimates of the lateral pressure exerted by the grafted polymer in the brush and mushroom regimes, respectively...
  100. pmc Interaction of spin-labeled inhibitors of the vacuolar H+-ATPase with the transmembrane Vo-sector
    Neil Dixon
    University of Leeds, School of Chemistry and School of Biochemistry and Molecular Biology, Leeds, United Kingdom
    Biophys J 94:506-14. 2008
    ..The INDOL6 derivative, which is closest to the parent INDOL0 inhibitor, displays limited subtype specificity for the osteoclast V-ATPase, with an IC(50) in the 10-nanomolar range...
  101. ncbi request reprint Electron spin-echo studies of spin-labelled lipid membranes and free fatty acids interacting with human serum albumin
    Francesco De Simone
    Dipartimento di Fisica, Laboratorio di Biofisica Molecolare and UdR CNISM, Universita della Calabria, I 87036 Arcavacata di Rende, CS, Italy
    Biochim Biophys Acta 1768:1541-9. 2007
    ..Correspondingly, all segments of the bound chains are accessible to water, to rather similar extents...