Michael Mahler

Summary

Country: Germany

Publications

  1. ncbi request reprint Advances in B-cell epitope analysis of autoantigens in connective tissue diseases
    M Mahler
    Institute of Molecular Genetics, University of Heidelberg, Heidelberg, Germany
    Clin Immunol 107:65-79. 2003
  2. pmc International multicenter evaluation of autoantibodies to ribosomal P proteins
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Mainstr 85, 41469 Neuss, Germany
    Clin Vaccine Immunol 13:77-83. 2006
  3. pmc Effect of dsDNA binding to SmD-derived peptides on clinical accuracy in the diagnosis of systemic lupus erythematosus
    Michael Mahler
    Development and Production, Dr Fooke Laboratorien, Neuss 41469, Germany
    Arthritis Res Ther 9:R68. 2007
  4. ncbi request reprint Novel aspects of autoantibodies to the PM/Scl complex: clinical, genetic and diagnostic insights
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Mainstr 85, 41469 Neuss, Germany
    Autoimmun Rev 6:432-7. 2007
  5. pmc Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Arthritis Res Ther 10:R131. 2008
  6. pmc Clinical evaluation of autoantibodies to a novel PM/Scl peptide antigen
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Arthritis Res Ther 7:R704-13. 2005
  7. pmc Improved serological differentiation between systemic lupus erythematosus and mixed connective tissue disease by use of an SmD3 peptide-based immunoassay
    M Mahler
    Dr Fooke Laboratorien GmbH, Mainstr 85, 41469 Neuss, Germany
    Clin Diagn Lab Immunol 12:107-13. 2005
  8. pmc Identification of a SmD3 epitope with a single symmetrical dimethylation of an arginine residue as a specific target of a subpopulation of anti-Sm antibodies
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Arthritis Res Ther 7:R19-29. 2005
  9. ncbi request reprint Technical and clinical evaluation of anti-ribosomal P protein immunoassays
    M Mahler
    Pharmacia Diagnostics, Freiburg, Germany
    J Clin Lab Anal 18:215-23. 2004
  10. pmc The changing landscape of the clinical value of the PM/Scl autoantibody system
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Mainstr, 41469 Neuss, Germany
    Arthritis Res Ther 11:106. 2009

Detail Information

Publications31

  1. ncbi request reprint Advances in B-cell epitope analysis of autoantigens in connective tissue diseases
    M Mahler
    Institute of Molecular Genetics, University of Heidelberg, Heidelberg, Germany
    Clin Immunol 107:65-79. 2003
    ..To illustrate the identification of epitope structure, clinically relevant autoantigens, including CENP-A, PM/Scl-100, fibrillarin, filaggrin, Ro-52, and dsDNA, are discussed as examples of each type of epitope...
  2. pmc International multicenter evaluation of autoantibodies to ribosomal P proteins
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Mainstr 85, 41469 Neuss, Germany
    Clin Vaccine Immunol 13:77-83. 2006
    ..We confirm previously reported associations of antiribosomal antibodies with clinical symptoms and serological findings. Remarkably, we found a lower occurrence of serositis in Rib-P-positive lupus patients...
  3. pmc Effect of dsDNA binding to SmD-derived peptides on clinical accuracy in the diagnosis of systemic lupus erythematosus
    Michael Mahler
    Development and Production, Dr Fooke Laboratorien, Neuss 41469, Germany
    Arthritis Res Ther 9:R68. 2007
    ..Taken together, the data indicate that both SmD peptide ELISAs represent accurate assays and may be used as important standards for the detection of anti-Sm antibodies...
  4. ncbi request reprint Novel aspects of autoantibodies to the PM/Scl complex: clinical, genetic and diagnostic insights
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Mainstr 85, 41469 Neuss, Germany
    Autoimmun Rev 6:432-7. 2007
    ..The present review focuses on recent insights on anti-PM/Scl autoantibodies with special emphasis on clinical, genetic and diagnostic aspects...
  5. pmc Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Arthritis Res Ther 10:R131. 2008
    ..The primary goal of this study was to determine the sensitivity of indirect immunofluorescence using conventional HEp-2 substrates in the detection of sera with ribosomal P antibodies as detected by other immunoassays...
  6. pmc Clinical evaluation of autoantibodies to a novel PM/Scl peptide antigen
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Arthritis Res Ther 7:R704-13. 2005
    ..The anti-PM1-alpha ELISA thus offers a new serological marker to diagnose and discriminate different systemic autoimmune disorders...
  7. pmc Improved serological differentiation between systemic lupus erythematosus and mixed connective tissue disease by use of an SmD3 peptide-based immunoassay
    M Mahler
    Dr Fooke Laboratorien GmbH, Mainstr 85, 41469 Neuss, Germany
    Clin Diagn Lab Immunol 12:107-13. 2005
    ..Finally, we conclude that the recently identified SmD peptide containing a symmetrical dimethylarginine at position 112 of D3 represents a promising tool for the detection of a highly specific subpopulation of anti-Sm antibodies...
  8. pmc Identification of a SmD3 epitope with a single symmetrical dimethylation of an arginine residue as a specific target of a subpopulation of anti-Sm antibodies
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Arthritis Res Ther 7:R19-29. 2005
    ..Thus, anti-SMP detection using ELISA represents a new serological marker with which to diagnose and discriminate between systemic autoimmune disorders...
  9. ncbi request reprint Technical and clinical evaluation of anti-ribosomal P protein immunoassays
    M Mahler
    Pharmacia Diagnostics, Freiburg, Germany
    J Clin Lab Anal 18:215-23. 2004
    ..The Varelisa Rib-P profile and the addressable laser bead Rib-P assays represent promising tools and platforms for the detection of anti-Rib-P antibodies in the future...
  10. pmc The changing landscape of the clinical value of the PM/Scl autoantibody system
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Mainstr, 41469 Neuss, Germany
    Arthritis Res Ther 11:106. 2009
    ..This report will hopefully trigger systematic multi-centre studies to confirm and/or elucidate the novel line immunoassay and clinical associations...
  11. ncbi request reprint Characterization of the human autoimmune response to the major C-terminal epitope of the ribosomal P proteins
    M Mahler
    Pharmacia Diagnostics, Munzingerstrasse 7, 79111 Freiburg, Germany
    J Mol Med (Berl) 81:194-204. 2003
    ..The role of the major Rib-P epitope in the development of anti-ribosomal P antibodies and in the pathogenesis of SLE remains a subject of further investigation...
  12. doi request reprint Anti-Scl-70 (topo-I) antibodies in SLE: Myth or reality?
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Autoimmun Rev 9:756-60. 2010
    ..Therefore, our objective was to analyse the prevalence of ATA in different disease conditions with a special focus on SLE employing a systematic literature review (meta-analysis) in combination with our experimental investigations...
  13. ncbi request reprint Serological and clinical characterization of anti-dsDNA and anti-PM/Scl double-positive patients
    M Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Ann N Y Acad Sci 1109:311-21. 2007
    ..Clinically, anti-dsDNA(+)/anti-PM/Scl(+) patients may define a small subgroup of SLE patients with additional features of systemic sclerosis...
  14. doi request reprint PM1-Alpha ELISA: the assay of choice for the detection of anti-PM/Scl autoantibodies?
    Michael Mahler
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Autoimmun Rev 8:373-8. 2009
    ..In summary, the data derived from individual studies suggest that PM1-Alpha may become the "gold standard" for the detection of anti-PM/Scl antibodies...
  15. pmc Clinical and serological evaluation of a novel CENP-A peptide based ELISA
    Michael Mahler
    Dr Fooke Laboratorien, Mainstrasse 85, 41469 Neuss, Germany
    Arthritis Res Ther 12:R99. 2010
    ..Historically, ACA were detected by indirect immunofluorescence (IIF) on HEp-2 cells and confirmed by immunoassays using recombinant CENP-B. The objective of this study was to evaluate a novel CENP-A peptide ELISA...
  16. doi request reprint Epitope specificity and significance in systemic autoimmune diseases
    Michael Mahler
    Dr Fooke Laboratorien, Neuss, Germany
    Ann N Y Acad Sci 1183:267-87. 2010
    ....
  17. ncbi request reprint Development of a CENP-A/CENP-B-specific immune response in a patient with systemic sclerosis
    Michael Mahler
    Institute of Molecular Genetics, University of Heidelberg, Heidelberg, Germany
    Arthritis Rheum 46:1866-72. 2002
    ..Whether the D-penicillamine treatment received by this patient was involved in the triggering of this cascade remains a matter of speculation...
  18. doi request reprint Third generation anti-citrullinated peptide antibody assay is a sensitive marker in rheumatoid factor negative rheumatoid arthritis
    Andreas Swart
    Center for Rheumatic Diseases, Neuss, Germany
    Clin Chim Acta 414:266-72. 2012
    ..We compared 2 anti-citrullinated protein antibody (ACPA) assays using a routine patient cohort...
  19. ncbi request reprint Diverse humoral autoimmunity to the ribosomal P proteins in systemic lupus erythematosus and hepatitis C virus infection
    K Kessenbrock
    University of Heidelberg, Heidelberg, Germany
    J Mol Med (Berl) 85:953-9. 2007
    ..In contrast, anti-Rib-P autoantibodies in HCV infection mainly recognize epitopes within the N-terminal half of ribosomal P2...
  20. doi request reprint Anti-centromere antibodies in a large cohort of systemic sclerosis patients: comparison between immunofluorescence, CENP-A and CENP-B ELISA
    Michael Mahler
    Dr Fooke Laboratorien, Neuss, Germany
    Clin Chim Acta 412:1937-43. 2011
    ..The objective of this study was to compare the detection of ACA by IIF to CENP-A and a CENP-B ELISA in a large cohort of SSc patients...
  21. ncbi request reprint Experimental Helicobacter pylori infection induces antral-predominant, chronic active gastritis in hispid cotton rats (Sigmodon hispidus)
    Michael Mahler
    Institute for Laboratory Animal Science and Central Animal Facility, Medical School Hannover, Hannover, Germany
    Helicobacter 10:332-44. 2005
    ..The hispid cotton rat has proven to be an excellent animal model for a variety of human infectious disease agents. This study was performed to evaluate the use of the cotton rat as a model of Helicobacter pylori infection...
  22. ncbi request reprint A population of autoantibodies against a centromere-associated protein A major epitope motif cross-reacts with related cryptic epitopes on other nuclear autoantigens and on the Epstein-Barr nuclear antigen 1
    M Mahler
    Institute of Molecular Genetics, University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany
    J Mol Med 79:722-31. 2001
    ..The presence of such an ubiquitous structure on autoantigens suggests a novel peptide-driven mechanism for the evolution of autoantibodies...
  23. doi request reprint Latest update on the Ro/SS-A autoantibody system
    J Schulte-Pelkum
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    Autoimmun Rev 8:632-7. 2009
    ..It was concluded that Ro60 (SS-A) and Ro52 represent two distinct autoantibody systems and that separate detection is desirable in a clinical diagnostic setting...
  24. ncbi request reprint Identification of an alpha-helical epitope region on the PM/Scl-100 autoantigen with structural homology to a region on the heterochromatin p25beta autoantigen using immobilized overlapping synthetic peptides
    M Bluthner
    Institute of Molecular Genetics, University of Heidelberg, Germany
    J Mol Med (Berl) 78:47-54. 2000
    ....
  25. ncbi request reprint Development and evaluation of a rapid assay for the diagnosis of immunoglobulin E-mediated type I allergies
    R Lucassen
    Dr Fooke Laboratorien GmbH, Neuss, Germany
    J Investig Allergol Clin Immunol 18:223-4. 2008
  26. ncbi request reprint Clinical and serological associations of autoantibodies to GW bodies and a novel cytoplasmic autoantigen GW182
    Theophany Eystathioy
    Department of Medicine, University of Calgary, 3330 Hospital Dr N W, Calgary, AB, T2N 4N1, Canada
    J Mol Med (Berl) 81:811-8. 2003
    ..None of the GW182 epitopes had significant sequence similarities to other known proteins. GW182 represents a new category of ribonucleoprotein autoantigens...
  27. ncbi request reprint Identification of the B-cell epitopes of the early endosome antigen 1 (EEA1)
    Sanja Selak
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, T2N 4N1 Alberta, Canada
    Clin Immunol 109:154-64. 2003
    ....
  28. ncbi request reprint Synthetic peptides: the future of patient management in systemic rheumatic diseases?
    Kai Kessenbrock
    Department of Neuroimmunology, Max Planck Institute of Neurobiology, Martinsried, Germany
    Curr Med Chem 14:2831-8. 2007
    ..This may lead to a new scientific research area with high impact on the development of diagnostic and therapeutic products, to the area of peptide engineering and "theranostics"...
  29. ncbi request reprint A panel of monoclonal antibodies to cytoplasmic GW bodies and the mRNA binding protein GW182
    Theophany Eystathioy
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    Hybrid Hybridomics 22:79-86. 2003
    ..These MAbs are valuable tools for cell biologists and pathologists to study the location and function of the novel GW182 protein in tissue culture cells, as well as cryopreserved or archived tissues...
  30. ncbi request reprint Generation of an HFRS patient-derived neutralizing recombinant antibody to Hantaan virus G1 protein and definition of the neutralizing domain
    Mifang Liang
    Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China
    J Med Virol 69:99-107. 2003
    ..These results are the first to define a neutralizing epitope on the G1 protein of HTNV using an antibody derived from an HFRS patient...