A A Brakhage

Summary

Affiliation: Leibniz Institute for Natural Product Research and Infection Biology
Country: Germany

Publications

  1. ncbi request reprint Regulation of penicillin biosynthesis in filamentous fungi
    Axel A Brakhage
    University of Hannover, Institute of Microbiology, Schneiderberg 50, 30167 Hannover, Germany
    Adv Biochem Eng Biotechnol 88:45-90. 2004
  2. pmc Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron
    Peter Hortschansky
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, and Friedrich Schiller University Jena, Jena, Germany
    EMBO J 26:3157-68. 2007
  3. pmc Production of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA
    Sandra Bruns
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knoell Institute HKI, Jena, Germany
    PLoS Pathog 6:e1000873. 2010
  4. pmc The CCAAT-binding complex coordinates the oxidative stress response in eukaryotes
    Marcel Thön
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, Beutenbergstrasse 11a, D 07745 Jena, Germany
    Nucleic Acids Res 38:1098-113. 2010
  5. pmc Pyomelanin formation in Aspergillus fumigatus requires HmgX and the transcriptional activator HmgR but is dispensable for virulence
    Sophia Keller
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Jena, Germany
    PLoS ONE 6:e26604. 2011
  6. pmc The MAP kinase MpkA controls cell wall integrity, oxidative stress response, gliotoxin production and iron adaptation in Aspergillus fumigatus
    Radhika Jain
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, Beutenbergstrasse 11a, D 07745 Jena, Germany
    Mol Microbiol 82:39-53. 2011
  7. pmc Conidial Dihydroxynaphthalene Melanin of the Human Pathogenic Fungus Aspergillus fumigatus Interferes with the Host Endocytosis Pathway
    Andreas Thywißen
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute Jena, Germany
    Front Microbiol 2:96. 2011
  8. pmc Systems biology of fungal infection
    Fabian Horn
    Systems Biology Bioinformatics, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute Jena, Germany
    Front Microbiol 3:108. 2012
  9. pmc Extrinsic extracellular DNA leads to biofilm formation and colocalizes with matrix polysaccharides in the human pathogenic fungus Aspergillus fumigatus
    Iordana Shopova
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute Jena, Germany Department of Microbiology and Molecular Biology, Institute of Microbiology, Friedrich Schiller University Jena, Germany
    Front Microbiol 4:141. 2013
  10. pmc Comparison of transcriptome technologies in the pathogenic fungus Aspergillus fumigatus reveals novel insights into the genome and MpkA dependent gene expression
    Sebastian Müller
    Department of Systems Biology Bioinformatics, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute, Beutenbergstr 11a, Jena 07745, Germany
    BMC Genomics 13:519. 2012

Collaborators

Detail Information

Publications69

  1. ncbi request reprint Regulation of penicillin biosynthesis in filamentous fungi
    Axel A Brakhage
    University of Hannover, Institute of Microbiology, Schneiderberg 50, 30167 Hannover, Germany
    Adv Biochem Eng Biotechnol 88:45-90. 2004
    ..Furthermore, such investigations have contributed to the elucidation of signals leading to the production of penicillin and can be expected to have a major impact on rational strain improvement programs...
  2. pmc Interaction of HapX with the CCAAT-binding complex--a novel mechanism of gene regulation by iron
    Peter Hortschansky
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, and Friedrich Schiller University Jena, Jena, Germany
    EMBO J 26:3157-68. 2007
    ..Remarkably, CBC-mediated regulation has an inverse impact on the expression of the same gene set in A. nidulans, compared with Saccharomyces cerevisae...
  3. pmc Production of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA
    Sandra Bruns
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knoell Institute HKI, Jena, Germany
    PLoS Pathog 6:e1000873. 2010
    ..fumigatus, but suggest that it does not play a major role in killing this fungus. Instead, NETs may have a fungistatic effect and may prevent further spreading...
  4. pmc The CCAAT-binding complex coordinates the oxidative stress response in eukaryotes
    Marcel Thön
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, Beutenbergstrasse 11a, D 07745 Jena, Germany
    Nucleic Acids Res 38:1098-113. 2010
    ..The underlying fine-tuned mechanism very likely represents a general feature of the CCAAT-binding complexes in eukaryotes...
  5. pmc Pyomelanin formation in Aspergillus fumigatus requires HmgX and the transcriptional activator HmgR but is dispensable for virulence
    Sophia Keller
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Jena, Germany
    PLoS ONE 6:e26604. 2011
    ..Thus, HmgX is supposed to function as an accessory factor to mediate specific activity of HppD. Remarkably, the ability to degrade tyrosine and to form pyomelanin is dispensable for virulence of A. fumigatus in a murine infection model...
  6. pmc The MAP kinase MpkA controls cell wall integrity, oxidative stress response, gliotoxin production and iron adaptation in Aspergillus fumigatus
    Radhika Jain
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, Beutenbergstrasse 11a, D 07745 Jena, Germany
    Mol Microbiol 82:39-53. 2011
    ..Based on our data, we propose that MpkA fine-tunes the balance between stress response and energy consuming cellular processes...
  7. pmc Conidial Dihydroxynaphthalene Melanin of the Human Pathogenic Fungus Aspergillus fumigatus Interferes with the Host Endocytosis Pathway
    Andreas Thywißen
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute Jena, Germany
    Front Microbiol 2:96. 2011
    ..fumigatus based on the finding that addition of bafilomycin which inhibits vATPase, led to complete inhibition of the acidification whereas the fusion of phagosomes containing wild-type conidia and lysosomes was not affected...
  8. pmc Systems biology of fungal infection
    Fabian Horn
    Systems Biology Bioinformatics, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute Jena, Germany
    Front Microbiol 3:108. 2012
    ....
  9. pmc Extrinsic extracellular DNA leads to biofilm formation and colocalizes with matrix polysaccharides in the human pathogenic fungus Aspergillus fumigatus
    Iordana Shopova
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute Jena, Germany Department of Microbiology and Molecular Biology, Institute of Microbiology, Friedrich Schiller University Jena, Germany
    Front Microbiol 4:141. 2013
    ..These results indicate that biofilm formation in A. fumigatus is shaped by certain substrates and in response to host environmental signals...
  10. pmc Comparison of transcriptome technologies in the pathogenic fungus Aspergillus fumigatus reveals novel insights into the genome and MpkA dependent gene expression
    Sebastian Müller
    Department of Systems Biology Bioinformatics, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute, Beutenbergstr 11a, Jena 07745, Germany
    BMC Genomics 13:519. 2012
    ..This kinase is involved in the cell wall integrity signalling pathway of A. fumigatus and essential for maintaining an intact cell wall in response to stress...
  11. doi request reprint Regulation of fungal secondary metabolism
    Axel A Brakhage
    Leibniz Institute for Natural Product Research and Infection Biology HKI, Beutenbergstrasse 11a, 07745 Jena, Germany
    Nat Rev Microbiol 11:21-32. 2013
    ....
  12. pmc Regulatory interactions for iron homeostasis in Aspergillus fumigatus inferred by a Systems Biology approach
    Jörg Linde
    Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute, Jena, Germany
    BMC Syst Biol 6:6. 2012
    ..Even though some regulatory interactions are known, we are still far from a complete understanding of the way iron homeostasis is regulated...
  13. pmc Comparative and functional genomics provide insights into the pathogenicity of dermatophytic fungi
    Anke Burmester
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute, Beutenbergstrasse 11a, Jena, 07745, Germany
    Genome Biol 12:R7. 2011
    ....
  14. pmc Integrative analysis of the heat shock response in Aspergillus fumigatus
    Daniela Albrecht
    Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Jena, Germany
    BMC Genomics 11:32. 2010
    ..fumigatus. The study was augmented by bioinformatical analysis of transcription factor binding sites (TFBSs) in the promoter region of genes whose corresponding proteins were differentially regulated upon heat shock...
  15. ncbi request reprint Systemic fungal infections caused by Aspergillus species: epidemiology, infection process and virulence determinants
    Axel A Brakhage
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, Beutenbergstrasse 11a, D 07745 Jena, Germany
    Curr Drug Targets 6:875-86. 2005
    ..fumigatus. The review discusses aspects of the current knowledge of the infection process, mechanisms of protection of the fungus against immune effector cells, and virulence determinants of A. fumigatus...
  16. ncbi request reprint Activation of fungal silent gene clusters: a new avenue to drug discovery
    Axel A Brakhage
    Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, Friedrich Schiller University, Jena, Germany
    Prog Drug Res 66:1, 3-12. 2008
    ..The activation of these gene clusters by genetic engineering will lead to the discovery of many so far unknown products and therefore represents a novel avenue to drug discovery...
  17. doi request reprint Interaction of phagocytes with filamentous fungi
    Axel A Brakhage
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute HKI, Beutenbergstrasse 11a, 07745 Jena, Germany
    Curr Opin Microbiol 13:409-15. 2010
    ..In addition, A. fumigatus interferes with the innate immunity, with both the complement system and defense mechanisms of phagocytes, thereby evading at least in part the innate immune system...
  18. ncbi request reprint Aspergillus fumigatus conidial pigment and cAMP signal transduction: significance for virulence
    A A Brakhage
    Leibniz lnstitute for Natural Products Research and Infection Biology Hans Knoell lnstitute, Department of Molecular and Applied Microbiology, Jena, Germany
    Med Mycol 43:S75-82. 2005
    ..However, besides its contribution to the biosynthesis of DHN-like melanin, PKSP also appears to be involved in the formation of another compound which is immunosuppressive...
  19. ncbi request reprint Evolution of beta-lactam biosynthesis genes and recruitment of trans-acting factors
    Axel A Brakhage
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Products Research and Infection Biology, Hans Knoell Institute, Beutenbergstrasse 11a, D 07745 Jena, Germany
    Phytochemistry 66:1200-10. 2005
    ..Alternatively, it is conceivable that only a part of the beta-lactam biosynthesis gene cluster was transferred to some fungi, e.g., the acvA and ipnA gene without a regulatory gene...
  20. doi request reprint Fungal secondary metabolites - strategies to activate silent gene clusters
    Axel A Brakhage
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute HKI, Beutenbergstr 11a, 07745 Jena, Germany
    Fungal Genet Biol 48:15-22. 2011
    ..These simulation strategies will help discover new natural products in the future, and may also provide fundamental new insights into microbial communication...
  21. doi request reprint Aspects on evolution of fungal beta-lactam biosynthesis gene clusters and recruitment of trans-acting factors
    Axel A Brakhage
    Department of Molecular and Applied Microbiology, University of Jena, Jena, Germany
    Phytochemistry 70:1801-11. 2009
    ..We propose that during the assembly of aatA and acvA-ipnA into a single gene cluster, recruitment of transcriptional regulators occurred along with acquisition of the duplicated aatA ancestor gene and its cis-acting sites...
  22. doi request reprint Missing values in gel-based proteomics
    Daniela Albrecht
    Research group Systems Biology Bioinformatics, Hans Knölle Institute, Jena, Germany
    Proteomics 10:1202-11. 2010
    ..We summarize reasons for missing values and explore their distribution in data sets. We also provide a comparison and evaluation of hitherto proposed imputation methods for gel-based proteomics data...
  23. pmc Protein kinase A regulates growth, sporulation, and pigment formation in Aspergillus fumigatus
    Christina Grosse
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Friedrich Schiller University Jena, 07745 Jena, Germany
    Appl Environ Microbiol 74:4923-33. 2008
    ..In contrast, proteins which were involved in nucleotide and amino acid biosynthesis were downregulated, as were enzymes involved in catabolism of carbon sources other than glucose...
  24. doi request reprint Proteome analysis for pathogenicity and new diagnostic markers for Aspergillus fumigatus
    Olaf Kniemeyer
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute HKI, Jena, Germany
    Med Mycol 47:S248-54. 2009
    ..Thus, besides pathogenicity studies, proteomics also delivers the tools to screen for new antigens which could improve the diagnosis of diseases caused by A. fumigatus...
  25. pmc Environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans
    Judith Behnsen
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Jena, Germany
    PLoS Pathog 3:e13. 2007
    ..This could explain why "delocalized" Aspergillus infections such as hematogeneous spread are almost uncontrollable diseases, even in immunocompetent individuals...
  26. doi request reprint FungiFun: a web-based application for functional categorization of fungal genes and proteins
    Steffen Priebe
    Research group Systems Biology Bioinformatics, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute HKI, Jena, Germany
    Fungal Genet Biol 48:353-8. 2011
    ..FungiFun is freely accessible at https://www.omnifung.hki-jena.de/FungiFun/...
  27. pmc The Aspergillus fumigatus transcriptional regulator AfYap1 represents the major regulator for defense against reactive oxygen intermediates but is dispensable for pathogenicity in an intranasal mouse infection model
    Franziska Lessing
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knoell Institute, Jena, Germany
    Eukaryot Cell 6:2290-302. 2007
    ..fumigatus wild-type and DeltaAfyap1 mutant germlings by human neutrophilic granulocytes worked equally well irrespective of whether the ROI scavenger glutathione or an NADPH-oxidase inhibitor was added to the cells...
  28. pmc Intimate bacterial-fungal interaction triggers biosynthesis of archetypal polyketides in Aspergillus nidulans
    Volker Schroeckh
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute, 07745 Jena, Germany
    Proc Natl Acad Sci U S A 106:14558-63. 2009
    ....
  29. ncbi request reprint Identification of the novel penicillin biosynthesis gene aatB of Aspergillus nidulans and its putative evolutionary relationship to this fungal secondary metabolism gene cluster
    Petra Spröte
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, 07745 Jena, Germany
    Mol Microbiol 70:445-61. 2008
    ..Our findings suggest that aatB is a paralogue of aatA. They extend the model of evolution of the penicillin biosynthesis gene cluster by recruitment of a biosynthesis gene and its cis-regulatory sites upon gene duplication...
  30. ncbi request reprint Characterisation of the laccase-encoding gene abr2 of the dihydroxynaphthalene-like melanin gene cluster of Aspergillus fumigatus
    Venelina Sugareva
    Department of Microbiology and Molecular Biology, Friedrich Schiller University, Jena, Germany
    Arch Microbiol 186:345-55. 2006
    ..An abr2p-lacZ gene fusion was expressed during sporulation, but not during vegetative growth confirming the pattern of laccase activity due to Abr2...
  31. doi request reprint Functional genomic profiling of Aspergillus fumigatus biofilm reveals enhanced production of the mycotoxin gliotoxin
    Sandra Bruns
    Department of Molecular and Applied Microbiology, Hans Knöll Institute, Jena, Germany
    Proteomics 10:3097-107. 2010
    ..It may confer A. fumigatus protection from the host immune system and also enable its survival and persistence in chronic lung infections such as aspergilloma...
  32. doi request reprint Proteome profiling and functional classification of intracellular proteins from conidia of the human-pathogenic mold Aspergillus fumigatus
    Janka Teutschbein
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute HKI, Jena, Germany
    J Proteome Res 9:3427-42. 2010
    ..The generated data lay the foundations for further proteomic analyses and a better understanding of fungal pathogenesis...
  33. ncbi request reprint Optimisation of a 2-D gel electrophoresis protocol for the human-pathogenic fungus Aspergillus fumigatus
    Olaf Kniemeyer
    Department of Microbiology and Molecular Biology, Friedrich Schiller University, Jena, Germany
    Curr Genet 49:178-89. 2006
    ..In A. fumigatus, differential patterns of enzymes of the gluconeogenesis, glyoxylate cycle and ethanol degradation pathway during growth on glucose and ethanol were observed...
  34. pmc Secreted Aspergillus fumigatus protease Alp1 degrades human complement proteins C3, C4, and C5
    Judith Behnsen
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, Beutenbergstrasse 11a, Jena, Germany
    Infect Immun 78:3585-94. 2010
    ..Thus, the protease Alp1 is responsible for the observed cleavage and degrades a broad range of different substrates. In summary, we identified a novel mechanism in A. fumigatus that contributes to evasion from the host complement attack...
  35. pmc Production of pyomelanin, a second type of melanin, via the tyrosine degradation pathway in Aspergillus fumigatus
    Jeannette Schmaler-Ripcke
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute and Friedrich Schiller University Jena, Beutenbergstrasse 11a, 07745 Jena, Germany
    Appl Environ Microbiol 75:493-503. 2009
    ..fumigatus. Homogentisic acid is the major intermediate, and the L-tyrosine degradation pathway leading to pyomelanin is similar to that in humans leading to alkaptomelanin...
  36. ncbi request reprint Two-dimensional proteome reference maps for the human pathogenic filamentous fungus Aspergillus fumigatus
    Martin Vödisch
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knoell Institute HKI, Jena, Germany
    Proteomics 9:1407-15. 2009
    ..Furthermore, we established a protocol for the isolation of mitochondria of A. fumigatus and developed a mitochondrial proteome reference map. 147 proteins represented by 234 spots were identified...
  37. ncbi request reprint Apoptosis inhibition of alveolar macrophages upon interaction with conidia of Aspergillus fumigatus
    Katrin Volling
    Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knoell Institute HKI, and Friedrich Schiller University Jena, Jena, Germany
    FEMS Microbiol Lett 275:250-4. 2007
    ..This unexpected result was due to inhibition of caspase 3 by a yet unknown mechanism...
  38. ncbi request reprint The mitogen-activated protein kinase MpkA of Aspergillus fumigatus regulates cell wall signaling and oxidative stress response
    Vito Valiante
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute HKI, Beutenbergstrasse 11a, 07745 Jena, Germany
    Fungal Genet Biol 45:618-27. 2008
    ..The expression of mpkA was induced by cell wall damaging conditions. Despite its involvement in cell wall signaling no influence on virulence of the deletion of mpkA was observed in a murine infection model...
  39. pmc Contribution of peroxisomes to penicillin biosynthesis in Aspergillus nidulans
    Petra Spröte
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, Jena, Germany
    Eukaryot Cell 8:421-3. 2009
    ..This is in contrast to the effects of IAT mislocalization in Penicillium chrysogenum...
  40. doi request reprint The MpkA MAP kinase module regulates cell wall integrity signaling and pyomelanin formation in Aspergillus fumigatus
    Vito Valiante
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute HKI, Beutenbergstrasse 11a, 07745 Jena, Germany
    Fungal Genet Biol 46:909-18. 2009
    ..Interestingly, this MAP kinase module affects the formation of pyomelanin derived from tyrosine degradation...
  41. pmc The opportunistic human pathogenic fungus Aspergillus fumigatus evades the host complement system
    Judith Behnsen
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute, Jena, Germany
    Infect Immun 76:820-7. 2008
    ..These data show that A. fumigatus conidia bind to complement regulators, and these bound host regulators may contribute to evasion of a host complement attack...
  42. pmc Aspergillus fumigatus does not require fatty acid metabolism via isocitrate lyase for development of invasive aspergillosis
    Felicitas Schöbel
    Leibniz Institute for Natural Products Research and Infection Biology, Beutenbergstr 11a, 07745 Jena, Germany
    Infect Immun 75:1237-44. 2007
    ..Therefore, an antifungal drug inhibiting fungal isocitrate lyases, postulated to act against Candida infections, is assumed to be ineffective against A. fumigatus...
  43. ncbi request reprint The CCAAT-binding complex of eukaryotes: evolution of a second NLS in the HapB subunit of the filamentous fungus Aspergillus nidulans despite functional conservation at the molecular level between yeast, A.nidulans and human
    André Tüncher
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knoell Institute, Beutenbergstrasse 11a, D 07745 Jena, Germany
    J Mol Biol 352:517-33. 2005
    ..nidulans hapB mutant was complemented, at least in part, by both the human NF-YA and S.cerevisiae Hap2p this finding suggests that the piggy-back mechanism of nuclear transport found for A.nidulans is conserved in yeast and human...
  44. ncbi request reprint The light-dependent regulator velvet A of Aspergillus nidulans acts as a repressor of the penicillin biosynthesis
    Petra Spröte
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, Beutenbergstrasse 11a, 07745 Jena, Germany
    Arch Microbiol 188:69-79. 2007
    ..These findings were confirmed by the analysis of a regulatable niiAp-veA gene fusion. Under niiAp-inducing conditions, penicillin titres and acvAp-uidA expression were much lower than in untransformed wild-type strains...
  45. ncbi request reprint Analysis of mating-dependent transcription of Blakeslea trispora carotenoid biosynthesis genes carB and carRA by quantitative real-time PCR
    André D Schmidt
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Products Research and Infection Biology, Hans Knoell Institute, Jena, Germany
    Appl Microbiol Biotechnol 67:549-55. 2005
    ..Transcription levels of both carB and carRA were strongly induced only under mating conditions. These data suggest that beta-carotene production in B. trispora is due to increased transcription of the biosynthesis genes carB and carRA...
  46. pmc Activation of a silent fungal polyketide biosynthesis pathway through regulatory cross talk with a cryptic nonribosomal peptide synthetase gene cluster
    Sebastian Bergmann
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute HKI, Beutenbergstrasse 11a, 07745 Jena, Germany
    Appl Environ Microbiol 76:8143-9. 2010
    ..To the best of our knowledge, this is the first report of regulatory cross talk between two biosynthesis gene clusters located on different chromosomes...
  47. ncbi request reprint Transannular disulfide formation in gliotoxin biosynthesis and its role in self-resistance of the human pathogen Aspergillus fumigatus
    Daniel H Scharf
    Leibniz Institute for Natural Product Research and Infection Biology, Beutenbergstrasse 11a, 07745 Jena, Germany
    J Am Chem Soc 132:10136-41. 2010
    ..GliT thus not only represents the prototype of ETP-forming enzymes in eukaryotes but also delineates a novel mechanism for self-resistance...
  48. ncbi request reprint Genomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus nidulans
    Sebastian Bergmann
    Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute, Beutenbergstr 11a, 07745 Jena, Germany
    Nat Chem Biol 3:213-7. 2007
    ..Here we report a new strategy for the successful induction of a silent metabolic pathway in the important model organism Aspergillus nidulans, which led to the discovery of novel PKS-NRPS hybrid metabolites...
  49. pmc Protein kinase C (PkcA) of Aspergillus nidulans is involved in penicillin production
    Martina Herrmann
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology HKI, Beutenbergstrasse 11a, D 07745 Jena, Germany
    Appl Environ Microbiol 72:2957-70. 2006
    ..The involvement of Pkc in penicillin biosynthesis is also interesting in light of the fact that in the yeast Saccharomyces cerevisiae, Pkc plays a major role in maintaining cell integrity...
  50. pmc The cyclic AMP-dependent protein kinase a network regulates development and virulence in Aspergillus fumigatus
    Burghard Liebmann
    Institut fur Mikrobiologie, Universitat Hannover, Schneiderberg 50, D 30167 Hannover, Germany
    Infect Immun 72:5193-203. 2004
    ..Taken together, these findings indicate that the cAMP-PKA signal transduction pathway is required for A. fumigatus pathogenicity...
  51. ncbi request reprint Deletion of the Aspergillus fumigatus lysine biosynthesis gene lysF encoding homoaconitase leads to attenuated virulence in a low-dose mouse infection model of invasive aspergillosis
    Burghard Liebmann
    Institut fur Mikrobiologie, Universitat Hannover, Schneiderberg 50, 30167, Hannover, Germany
    Arch Microbiol 181:378-83. 2004
    ..In this murine model, the Delta lysF mutant was avirulent, suggesting that lysine biosynthesis, or at least a functional homoaconitase, is important for survival of A. fumigatus in vivo and a potential target for antifungal drugs...
  52. ncbi request reprint Biosynthesis of fungal melanins and their importance for human pathogenic fungi
    Kim Langfelder
    Institut fur Mikrobiologie, Universitat Hannover, Schneiderberg 50, 30167, Hannover, Germany
    Fungal Genet Biol 38:143-58. 2003
    ..In addition, in several fungi the production of fungal virulence factors, such as melanin, has been linked to a cAMP-dependent signaling pathway. Many of the components involved in the signaling pathway have been identified...
  53. pmc SreA-mediated iron regulation in Aspergillus fumigatus
    Markus Schrettl
    Divisions of Molecular Biology Biocenter, Medical University Innsbruck, Fritz Pregl Str 3, A 6020 Innsbruck, Austria
    Mol Microbiol 70:27-43. 2008
    ..As all detrimental effects of sreA disruption are restricted to iron-replete conditions these data underscore that A. fumigatus faces iron-depleted conditions during infection...
  54. ncbi request reprint Methylcitrate synthase from Aspergillus fumigatus is essential for manifestation of invasive aspergillosis
    Oumaima Ibrahim-Granet
    Aspergillus Unit, Institute Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Cell Microbiol 10:134-48. 2008
    ..fumigatus. Thus, blocking of methylcitrate synthase activity abrogates fungal growth and provides a suitable target for new antifungals...
  55. ncbi request reprint Functional characterization of the Aspergillus fumigatus calcineurin
    Márcia Eliana da Silva Ferreira
    Faculdade de Ciências Farmacêuticas de Ribeirão, Universidade de Sao Paulo, Brazil
    Fungal Genet Biol 44:219-30. 2007
    ..These results suggest that calcineurin is involved in the regulation of the PHO pathway in A. fumigatus. Our work on calcineurin opens new venues for the research on sensing and nutrient acquisition in A. fumigatus...
  56. ncbi request reprint Deletion of the gliP gene of Aspergillus fumigatus results in loss of gliotoxin production but has no effect on virulence of the fungus in a low-dose mouse infection model
    Claudio Kupfahl
    Institute for Medical Microbiology and Hygiene, Faculty for Clinical Medicine Mannheim of the University Heidelberg, Theodor Kutzer Ufer 1 3, 68167 Mannheim, Germany
    Mol Microbiol 62:292-302. 2006
    ..fumigatus. Gliotoxin is not required for pathogenicity of the fungus in immunocompromised mice, despite the fact that a reduced cytotoxicity of the culture supernatant of gliP deletion strains was demonstrated...
  57. ncbi request reprint Menacing mold: the molecular biology of Aspergillus fumigatus
    Axel A Brakhage
    Institut fur Mikrobiologie, Universitat Hannover, Schneiderberg 50, Germany
    Annu Rev Microbiol 56:433-55. 2002
    ..This review summarizes the current knowledge on the biology, molecular genetics, and genomics of A. fumigatus...
  58. ncbi request reprint Novel basic-region helix-loop-helix transcription factor (AnBH1) of Aspergillus nidulans counteracts the CCAAT-binding complex AnCF in the promoter of a penicillin biosynthesis gene
    Maria Louise Caruso
    Institut fur Mikrobiologie, Universitat Hannover, Schneiderberg 50, 30167 Hannover, Germany
    J Mol Biol 323:425-39. 2002
    ..The analysis of aatAp-lacZ expression in such a strain indicated that AnBH1 acts as a repressor of aatA gene expression and therefore counteracts the positive action of AnCF...
  59. ncbi request reprint PKSP-dependent reduction of phagolysosome fusion and intracellular kill of Aspergillus fumigatus conidia by human monocyte-derived macrophages
    Bernhard Jahn
    Institut fur Medizinische Mikrobiologie und Hygiene, Johannes Gutenberg Universitat Mainz, Hochhaus am Augustusplatz, D 55101 Mainz, Germany
    Cell Microbiol 4:793-803. 2002
    ..This finding might help explain the attenuated virulence of pksP mutant strains in a murine animal model and provides a conceptual frame to understand the virulence of A. fumigatus...
  60. ncbi request reprint A basic-region helix-loop-helix protein-encoding gene (devR) involved in the development of Aspergillus nidulans
    André Tüncher
    Institut fur Mikrobiologie, Universitat Hannover, Schneiderberg 50, D 30167 Hannover, Germany
    Mol Microbiol 52:227-41. 2004
    ..Taken together, devR encodes a bHLH regulatory protein that is part of the tcsA signal transduction network and required for development under standard growth conditions...
  61. ncbi request reprint Phagocytosis of Aspergillus fumigatus conidia by murine macrophages involves recognition by the dectin-1 beta-glucan receptor and Toll-like receptor 2
    Kathrin Luther
    Max von Pettenkofer Institute, Ludwig Maximilians University, Munich, Germany
    Cell Microbiol 9:368-81. 2007
    ..Additionally we found that TLR2 and the adaptor protein MyD88 are required for efficient conidial phagocytosis, suggesting a link between the TLR2-mediated recognition of A. fumigatus and the phagocytic response...
  62. ncbi request reprint A single subunit of a heterotrimeric CCAAT-binding complex carries a nuclear localization signal: piggy back transport of the pre-assembled complex to the nucleus
    Stefan Steidl
    Institut fur Mikrobiologie, Universitat Hannover, Schneiderberg 50, D 30167 Hannover, Germany
    J Mol Biol 342:515-24. 2004
    ..This enables the cell to provide equimolar concentrations of all subunits to the nucleus...
  63. ncbi request reprint Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole
    Márcia Eliana da Silva Ferreira
    Departamento de Ciencias Farmaceuticas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brazil
    Curr Genet 50:32-44. 2006
    ....
  64. ncbi request reprint Methylcitrate synthase from Aspergillus fumigatus. Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia
    Claudia Maerker
    Institute of Microbiology, University of Hannover, Germany
    FEBS J 272:3615-30. 2005
    ..Therefore, an impact of methylcitrate synthase in the infection process is discussed...
  65. ncbi request reprint Analysis of the regulation, expression, and localisation of the isocitrate lyase from Aspergillus fumigatus, a potential target for antifungal drug development
    Frank Ebel
    Max von Pettenkofer Institute, Munich, Germany
    Fungal Genet Biol 43:476-89. 2006
    ..Lipids, as potential substrates, were detected in conidia and macrophages. Additionally, germinating conidia within macrophages contain ICL, suggesting that the glyoxylate shunt might be a relevant target for development of antifungals...
  66. pmc The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus
    Márcia Eliana da Silva Ferreira
    Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Departamento de Ciencias Farmaceuticas, Universidade de Sao Paulo, Av do Cafe S N, CEP 14040 903, Ribeirao Preto, Sao Paulo, Brazil
    Eukaryot Cell 5:207-11. 2006
    ..fumigatus strain. Deletion of akuB(KU80) had no influence on pathogenicity in a low-dose murine infection model...
  67. ncbi request reprint Nuclear translocation of the heterotrimeric CCAAT binding factor of Aspergillus oryzae is dependent on two redundant localising signals in a single subunit
    Hideya Goda
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464 8601 Japan
    Arch Microbiol 184:93-100. 2005
    ..nidulans. The nuclear translocation of the A. oryzae complex was found to be dependent on two redundant localising signals in HapB...
  68. ncbi request reprint A novel method used to delete a new Aspergillus fumigatus ABC transporter-encoding gene
    Kim Langfelder
    Institut fur Mikrobiologie, Universitat Hannover, Am Schneiderberg 50, 30167 Hannover, Germany
    Curr Genet 41:268-74. 2002
    ..fumigatus knock-out mutant was generated, providing evidence that this method of generating gene deletions can also be used in A. fumigatus and significantly broadens our repertoire of molecular techniques to study A. fumigatus...