Zecha J, Meng C, Zolg D, Samaras P, Wilhelm M, Kuster B. Peptide Level Turnover Measurements Enable the Study of Proteoform Dynamics. Mol Cell Proteomics. 2018;17:974-992 pubmed publisher
Lee C, Wang D, Wilhelm M, Zolg D, Schmidt T, Schnatbaum K, et al
. Mining the Human Tissue Proteome for Protein Citrullination. Mol Cell Proteomics. 2018;17:1378-1391 pubmed publisher
Zolg D, Wilhelm M, Schmidt T, Médard G, Zerweck J, Knaute T, et al
. ProteomeTools: Systematic characterization of 21 post-translational protein modifications by LC-MS/MS using synthetic peptides. Mol Cell Proteomics. 2018;: pubmed publisher
..To our knowledge, this constitutes the first broad and systematic analysis of the LC-MS/MS properties of common and rare PTMs using synthetic peptides, leading to direct applicable utility for bottom-up proteomic experiments. ..
da Silva B, Meng C, Helm D, Pachl F, Schiller J, Ibrahim E, et al
. Towards Understanding Male Infertility After Spinal Cord Injury Using Quantitative Proteomics. Mol Cell Proteomics. 2016;15:1424-34 pubmed publisher
..Together, our data highlights the molecular pathways hindering fertility in SCI and shed new light on other causes of male infertility. ..
Yu P, Hahne H, Wilhelm M, Kuster B. Ethylene glycol improves electrospray ionization efficiency in bottom-up proteomics. Anal Bioanal Chem. 2017;409:1049-1057 pubmed publisher
..Graphical Abstract Ethylene glycol substantially improves peptide ionization. ..
Ruprecht B, Wang D, Chiozzi R, Li L, Hahne H, Kuster B. Hydrophilic Strong Anion Exchange (hSAX) Chromatography Enables Deep Fractionation of Tissue Proteomes. Methods Mol Biol. 2017;1550:69-82 pubmed publisher
Ku X, Heinzlmeir S, Liu X, Médard G, Kuster B. A new chemical probe for quantitative proteomic profiling of fibroblast growth factor receptor and its inhibitors. J Proteomics. 2014;96:44-55 pubmed publisher
..The overall aim of such studies is to improve our understanding of how target as well as off-target profiles can be used to assess or predict the therapeutic efficacy of a drug. ..
Ruprecht B, Koch H, Domasinska P, Frejno M, Kuster B, Lemeer S. Optimized Enrichment of Phosphoproteomes by Fe-IMAC Column Chromatography. Methods Mol Biol. 2017;1550:47-60 pubmed publisher
..We show that the application of this protocol enables the selective (>90 %) identification of more than 10,000 unique phosphopeptides from 1 mg of HeLa digest within 2 h of measurement time (Q Exactive Plus). ..
Ruprecht B, Zecha J, Zolg D, Kuster B. High pH Reversed-Phase Micro-Columns for Simple, Sensitive, and Efficient Fractionation of Proteome and (TMT labeled) Phosphoproteome Digests. Methods Mol Biol. 2017;1550:83-98 pubmed publisher
..Importantly, this protocol is equally applicable to the fractionation of full proteome digests. ..