Ralf Kuppers

Summary

Country: Germany

Publications

  1. doi New insights in the biology of Hodgkin lymphoma
    Ralf Kuppers
    Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Essen, Germany
    Hematology Am Soc Hematol Educ Program 2012:328-34. 2012
  2. doi Laser-based microdissection of single cells from tissue sections and PCR analysis of rearranged immunoglobulin genes from isolated normal and malignant human B cells
    Ralf Kuppers
    Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
    Methods Mol Biol 971:49-63. 2013
  3. pmc Hodgkin lymphoma
    Ralf Kuppers
    Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
    J Clin Invest 122:3439-47. 2012
  4. doi The biology of Hodgkin's lymphoma
    Ralf Kuppers
    Institute of Cell Biology Tumour Research, University of Duisburg Essen, Medical School, Virchowstrasse 173, 45122 Essen, Germany
    Nat Rev Cancer 9:15-27. 2009
  5. doi Rare occurrence of biallelic CYLD gene mutations in classical Hodgkin lymphoma
    Annette Schmidt
    Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Essen, Germany
    Genes Chromosomes Cancer 49:803-9. 2010
  6. doi Pathogenesis of classical and lymphocyte-predominant Hodgkin lymphoma
    Roland Schmitz
    Institute for Cell Biology Tumor Research, Medical School, University of Duisburg Essen, Essen, Germany
    Annu Rev Pathol 4:151-74. 2009
  7. doi Analyzing primary Hodgkin and Reed-Sternberg cells to capture the molecular and cellular pathogenesis of classical Hodgkin lymphoma
    Enrico Tiacci
    Institute of Cell Biology Cancer Research, University of Duisburg Essen Medical School, Essen, Germany
    Blood 120:4609-20. 2012
  8. doi EBV transformation overrides gene expression patterns of B cell differentiation stages
    Dörte Siemer
    University of Duisburg Essen, Medical School, Institute for Cell Biology Tumor Research, Virchowst 173, 45122 Essen, Germany
    Mol Immunol 45:3133-41. 2008
  9. pmc Mutations in the genes coding for the NF-κB regulating factors IκBα and A20 are uncommon in nodular lymphocyte-predominant Hodgkin's lymphoma
    Martin A Schumacher
    Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
    Haematologica 95:153-7. 2010
  10. doi Mechanisms of aberrant GATA3 expression in classical Hodgkin lymphoma and its consequences for the cytokine profile of Hodgkin and Reed/Sternberg cells
    Jens Stanelle
    Institute of Cell Biology, University of Duisburg Essen Medical School, Essen, Germany
    Blood 116:4202-11. 2010

Collaborators

Detail Information

Publications36

  1. doi New insights in the biology of Hodgkin lymphoma
    Ralf Kuppers
    Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Essen, Germany
    Hematology Am Soc Hematol Educ Program 2012:328-34. 2012
    ..T cells represent a main population of infiltrating cells, and it appears that HRS cells both inhibit cytotoxic T cells efficiently and also receive survival signals from Th cells in direct contact with them...
  2. doi Laser-based microdissection of single cells from tissue sections and PCR analysis of rearranged immunoglobulin genes from isolated normal and malignant human B cells
    Ralf Kuppers
    Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
    Methods Mol Biol 971:49-63. 2013
    ..The approach is also useful to determine V, D, and J gene usage. Moreover, the presence and pattern of somatic Ig V gene mutations give valuable insight into the stage of differentiation of the B cells...
  3. pmc Hodgkin lymphoma
    Ralf Kuppers
    Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
    J Clin Invest 122:3439-47. 2012
    ..The dependency of HRS cells on microenvironmental interactions and deregulated signaling pathways may offer novel strategies for targeted therapies...
  4. doi The biology of Hodgkin's lymphoma
    Ralf Kuppers
    Institute of Cell Biology Tumour Research, University of Duisburg Essen, Medical School, Virchowstrasse 173, 45122 Essen, Germany
    Nat Rev Cancer 9:15-27. 2009
    ..Understanding the contribution of all of these changes to the pathogenesis of this disease is essential for the development of novel therapies...
  5. doi Rare occurrence of biallelic CYLD gene mutations in classical Hodgkin lymphoma
    Annette Schmidt
    Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Essen, Germany
    Genes Chromosomes Cancer 49:803-9. 2010
    ....
  6. doi Pathogenesis of classical and lymphocyte-predominant Hodgkin lymphoma
    Roland Schmitz
    Institute for Cell Biology Tumor Research, Medical School, University of Duisburg Essen, Essen, Germany
    Annu Rev Pathol 4:151-74. 2009
    ..Less is known about the pathogenesis of L&H cells in NLPHL, but increases in the activity of receptor tyrosine kinases, NF-kappaB, and JAK/STAT have also been detected...
  7. doi Analyzing primary Hodgkin and Reed-Sternberg cells to capture the molecular and cellular pathogenesis of classical Hodgkin lymphoma
    Enrico Tiacci
    Institute of Cell Biology Cancer Research, University of Duisburg Essen Medical School, Essen, Germany
    Blood 120:4609-20. 2012
    ..Finally, HRS cells display deregulated expression of several genes potentially highly relevant to lymphoma pathogenesis, including silencing of the apoptosis-inducer BIK and of INPP5D, an inhibitor of the PI3K-driven oncogenic pathway...
  8. doi EBV transformation overrides gene expression patterns of B cell differentiation stages
    Dörte Siemer
    University of Duisburg Essen, Medical School, Institute for Cell Biology Tumor Research, Virchowst 173, 45122 Essen, Germany
    Mol Immunol 45:3133-41. 2008
    ..Thus, EBV-infection deregulates multiple differentiation factors and processes in B cells, leading to a largely homogenous phenotype of EBV-infected B cells in latency III...
  9. pmc Mutations in the genes coding for the NF-κB regulating factors IκBα and A20 are uncommon in nodular lymphocyte-predominant Hodgkin's lymphoma
    Martin A Schumacher
    Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
    Haematologica 95:153-7. 2010
    ....
  10. doi Mechanisms of aberrant GATA3 expression in classical Hodgkin lymphoma and its consequences for the cytokine profile of Hodgkin and Reed/Sternberg cells
    Jens Stanelle
    Institute of Cell Biology, University of Duisburg Essen Medical School, Essen, Germany
    Blood 116:4202-11. 2010
    ..GATA3 activity significantly contributes to the typical cytokine secretion of and signaling in HRS cells, which presumably plays an essential role in HL pathogenesis...
  11. doi Recurrent deletions of the TNFSF7 and TNFSF9 genes in 19p13.3 in diffuse large B-cell and Burkitt lymphomas
    Rene Scholtysik
    Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Essen, Germany
    Int J Cancer 131:E830-5. 2012
    ..Thus, TNFSF7 and TNFSF9 deletions are recurrent genetic lesions in multiple types of human lymphomas...
  12. doi p53 protein expression in chronic lymphocytic leukemia
    Ludger Sellmann
    Department of Hematology, Medical Faculty, University of Duisburg Essen, Essen, Germany
    Leuk Lymphoma 53:1282-8. 2012
    ..Taken together, the differential expression of p53 isoforms could disrupt the p53 response and contribute to CLL pathogenesis...
  13. doi Genetic lesions of the TRAF3 and MAP3K14 genes in classical Hodgkin lymphoma
    Claudia Otto
    Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Essen, Germany
    Br J Haematol 157:702-8. 2012
    ....
  14. doi Molecular analysis of IgD-positive human germinal centres
    Claudia Müller
    Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Virchowstrasse 173, 45122 Essen, Germany
    Int Immunol 22:289-98. 2010
    ....
  15. pmc TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma
    Roland Schmitz
    Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, 45122 Essen, Germany
    J Exp Med 206:981-9. 2009
    ..The significantly higher frequency of TNFAIP3 mutations in EBV(-) than EBV(+) cHL suggests complementing functions of TNFAIP3 inactivation and EBV infection in cHL pathogenesis...
  16. pmc Origin and pathogenesis of nodular lymphocyte-predominant Hodgkin lymphoma as revealed by global gene expression analysis
    Verena Brune
    Institute for Cell Biology Tumor Research, University of Duisburg Essen Medical School, 45122 Essen, Germany
    J Exp Med 205:2251-68. 2008
    ..Thus, these findings shed new light on the nature of L&H cells, reveal several novel pathogenetic mechanisms in NLPHL, and may help in differential diagnosis and lead to novel therapeutic strategies...
  17. pmc Cellular origin and pathophysiology of chronic lymphocytic leukemia
    Marc Seifert
    Institute of Cell Biology Cancer Research, University of Duisburg Essen, Essen 45122, Germany
    J Exp Med 209:2183-98. 2012
    ..Finally, we identified deregulated proteins, including EBF1 and KLF transcription factors, that were not detected in previous comparisons of CLL and conventional B cells...
  18. ncbi Absence of measles virus genome and transcripts in Hodgkin-Reed/Sternberg cells of a cohort of Hodgkin lymphoma patients
    Ewerton Maggio
    Institute for Cell Biology Tumor Research, University of Duisburg Essen, Essen, Germany
    Int J Cancer 121:448-53. 2007
    ..In this cohort of German and Israeli HL patients--including cases previously typed as MV-positive--and HL-derived cell lines, there was no evidence of MV genome in the HRS cells...
  19. ncbi Molecular single-cell PCR analysis of rearranged immunoglobulin genes as a tool to determine the clonal composition of normal and malignant human B cells
    Ralf Kuppers
    Tumor Research, Institute for Cell Biology, University of Essen Medical School, Essen, Germany
    Methods Mol Biol 271:225-38. 2004
    ..As a large part of the V-gene rearrangements is amplified, the approach is also useful to address additional issues, such as V-, D-, and J-gene usage and the presence and pattern of somatic mutations...
  20. ncbi The Hodgkin and Reed/Sternberg cell
    Ralf Kuppers
    Institute for Cell Biology Tumor Research, University of Duisburg Essen, Medical School, Virchowstr 173, 45122 Essen, Germany
    Int J Biochem Cell Biol 37:511-7. 2005
    ..HRS or HRS-like cells are also found in several other diseases, e.g. as rare intermingled cells in some non-Hodgkin lymphomas and in infectious mononucleosis...
  21. doi A model for the development of human IgD-only B cells: Genotypic analyses suggest their generation in superantigen driven immune responses
    Marc Seifert
    Institute for Cell Biology Tumor Research, University of Duisburg Essen, Medical School, Virchowstr 173, 45122 Essen, Germany
    Mol Immunol 46:630-9. 2009
    ..IgD-only B cells may hence represent a unique B lineage subset generated in response to particular antigens...
  22. ncbi Transformation of BCR-deficient germinal-center B cells by EBV supports a major role of the virus in the pathogenesis of Hodgkin and posttransplantation lymphomas
    Dörte Bechtel
    Institute for Cell Biology Tumor Research, University of Duisburg Essen, Virchowstr 173, 45122 Essen, Germany
    Blood 106:4345-50. 2005
    ..The monoclonal LCLs represent valuable models for early stages of lymphoma development in classic HL and PTLD...
  23. ncbi Advances in biology, diagnostics, and treatment of Hodgkin's disease
    Ralf Kuppers
    Institute for Cell Biology, Tumor Research, University of Duisburg Essen, Germany
    Biol Blood Marrow Transplant 12:66-76. 2006
    ....
  24. pmc Molecular footprints of a germinal center derivation of human IgM+(IgD+)CD27+ B cells and the dynamics of memory B cell generation
    Marc Seifert
    Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, D 45122 Essen, Germany
    J Exp Med 206:2659-69. 2009
    ..This provides novel insights into the dynamics of GC reactions and the generation of a memory B cell repertoire...
  25. doi Telomeres and prognosis in patients with chronic lymphocytic leukaemia
    Ludger Sellmann
    Department of Haematology, University of Duisburg Essen, Essen, Germany
    Int J Hematol 93:74-82. 2011
    ..Short telomeres and high telomerase activity as occurs in some patients with CLL with a worse prognosis might be an ideal target for treatment with telomerase inhibitors...
  26. doi Mutation analysis of the TNFAIP3 (A20) tumor suppressor gene in CLL
    Claudia Philipp
    Institute of Cell Biology, University of Duisburg Essen, Medical School, Essen, Germany
    Int J Cancer 128:1747-50. 2011
    ..Therefore, A20 inactivation plays no significant role in the pathogenesis of CLL, and the recurrent deletion in CLL on 6q21-23, where TNFAIP3 is located, likely affects other gene(s)...
  27. ncbi Prognosis in follicular lymphoma--it's in the microenvironment
    Ralf Kuppers
    Institute for Cell Biology Tumor Research, University of Duisburg Essen Medical School, Essen, Germany
    N Engl J Med 351:2152-3. 2004
  28. doi Gene dosage effects in chronic lymphocytic leukemia
    Ludger Sellmann
    Department of Hematology, Medical Faculty, University of Duisburg Essen, Hufelandstrase 55, 45122 Essen, Germany
    Cancer Genet Cytogenet 203:149-60. 2010
    ..We did not confirm a consistent correlation between MIRN15A and MIRN16-1 expression levels and BCL2 protein levels, indicating a complex regulation of BCL2 expression...
  29. ncbi Reprogramming of the tumour B-cell phenotype in Hodgkin lymphoma
    Ralf Kuppers
    Institute for Cell Biology Tumor Research, University of Duisburg Essen, Medical School, 45122 Essen, Germany
    Trends Immunol 27:203-5. 2006
    ....
  30. ncbi Trisomy 19 is associated with trisomy 12 and mutated IGHV genes in B-chronic lymphocytic leukaemia
    Ludger Sellmann
    Department of Haematology, Medical Faculty, University of Duisburg Essen, Essen, Germany
    Br J Haematol 138:217-20. 2007
    ..Karyotypes of the present study and the literature identified a strong correlation to trisomy 18 in addition to trisomy 12. Trisomy 19 seems to be a secondary event in B-CLL with trisomy 12, mostly originating from mutated B cells...
  31. ncbi Prognostic significance of Epstein-Barr virus-infected cells in the bone marrow of patients with nasal natural killer/T cell lymphoma
    Ralf Kuppers
    Institute for Cell Biology, Tumor Research, University of Duisburg Essen, Medical School, Essen, Germany
    Haematologica 90:1011A. 2005
  32. ncbi The major subtypes of human B-cell lymphomas lack mutations in BCL-2 family member BAD
    Roland Schmitz
    Institute for Cell Biology Tumor Research, University of Duisburg Essen, Medical School, Essen, Germany
    Int J Cancer 119:1738-40. 2006
    ..No mutations were found in any of the cases. We conclude that mutations in the BAD gene do not play a role in the pathogenesis of the major subtypes of human B-cell lymphomas...
  33. doi Origin and pathogenesis of B cell lymphomas
    Marc Seifert
    Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
    Methods Mol Biol 971:1-25. 2013
    ..Many B cell malignancies derive from germinal center B cells, most likely because of the high proliferation rate of these cells and the high activity of mutagenic processes...
  34. pmc Detection of genomic aberrations in molecularly defined Burkitt's lymphoma by array-based, high resolution, single nucleotide polymorphism analysis
    Rene Scholtysik
    Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Virchowstr 173, 45122 Essen, Germany
    Haematologica 95:2047-55. 2010
    ..Knowledge about the genetic lesions that occur in Burkitt's lymphoma, besides the pathognomonic IG-MYC translocations, is limited...
  35. pmc B cells in chronically hepatitis C virus-infected individuals lack a virus-induced mutation signature in the TP53, CTNNB1, and BCL6 genes
    Felicia Anna Tucci
    Institute of Cell Biology Cancer Research, University of Duisburg Essen Medical School, Essen, Germany
    J Virol 87:2956-62. 2013
    ..Based on these findings, new targets have to be investigated as potential mediators of HCV-associated B-cell lymphomagenesis...
  36. ncbi Mechanisms of B-cell lymphoma pathogenesis
    Ralf Kuppers
    Institute for Cell Biology Tumor Research, University of Duisburg Essen, Medical School, Germany
    Nat Rev Cancer 5:251-62. 2005
    ..Recent insights into the lymphomagenic role of factors supplied by the microenvironment also offer new therapeutic strategies...