Research Topics
Genomes and GenesSpecies | Ralf KuppersSummaryCountry: Germany Publications
| Collaborators
|
Detail Information
Publications
New insights in the biology of Hodgkin lymphomaRalf Kuppers
Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Essen, Germany
Hematology Am Soc Hematol Educ Program 2012:328-34. 2012..T cells represent a main population of infiltrating cells, and it appears that HRS cells both inhibit cytotoxic T cells efficiently and also receive survival signals from Th cells in direct contact with them...- Laser-based microdissection of single cells from tissue sections and PCR analysis of rearranged immunoglobulin genes from isolated normal and malignant human B cellsRalf Kuppers
Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
Methods Mol Biol 971:49-63. 2013..The approach is also useful to determine V, D, and J gene usage. Moreover, the presence and pattern of somatic Ig V gene mutations give valuable insight into the stage of differentiation of the B cells... 
Hodgkin lymphomaRalf Kuppers
Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
J Clin Invest 122:3439-47. 2012..The dependency of HRS cells on microenvironmental interactions and deregulated signaling pathways may offer novel strategies for targeted therapies...- The biology of Hodgkin's lymphomaRalf Kuppers
Institute of Cell Biology Tumour Research, University of Duisburg Essen, Medical School, Virchowstrasse 173, 45122 Essen, Germany
Nat Rev Cancer 9:15-27. 2009..Understanding the contribution of all of these changes to the pathogenesis of this disease is essential for the development of novel therapies... - Rare occurrence of biallelic CYLD gene mutations in classical Hodgkin lymphomaAnnette Schmidt
Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Essen, Germany
Genes Chromosomes Cancer 49:803-9. 2010.... - Analyzing primary Hodgkin and Reed-Sternberg cells to capture the molecular and cellular pathogenesis of classical Hodgkin lymphomaEnrico Tiacci
Institute of Cell Biology Cancer Research, University of Duisburg Essen Medical School, Essen, Germany
Blood 120:4609-20. 2012..Finally, HRS cells display deregulated expression of several genes potentially highly relevant to lymphoma pathogenesis, including silencing of the apoptosis-inducer BIK and of INPP5D, an inhibitor of the PI3K-driven oncogenic pathway... - Pathogenesis of classical and lymphocyte-predominant Hodgkin lymphomaRoland Schmitz
Institute for Cell Biology Tumor Research, Medical School, University of Duisburg Essen, Essen, Germany
Annu Rev Pathol 4:151-74. 2009..Less is known about the pathogenesis of L&H cells in NLPHL, but increases in the activity of receptor tyrosine kinases, NF-kappaB, and JAK/STAT have also been detected... - Mutations in the genes coding for the NF-κB regulating factors IκBα and A20 are uncommon in nodular lymphocyte-predominant Hodgkin's lymphomaMartin A Schumacher
Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
Haematologica 95:153-7. 2010.... - Genetic lesions of the TRAF3 and MAP3K14 genes in classical Hodgkin lymphomaClaudia Otto
Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Essen, Germany
Br J Haematol 157:702-8. 2012.... - Mechanisms of aberrant GATA3 expression in classical Hodgkin lymphoma and its consequences for the cytokine profile of Hodgkin and Reed/Sternberg cellsJens Stanelle
Institute of Cell Biology, University of Duisburg Essen Medical School, Essen, Germany
Blood 116:4202-11. 2010..GATA3 activity significantly contributes to the typical cytokine secretion of and signaling in HRS cells, which presumably plays an essential role in HL pathogenesis... - Recurrent deletions of the TNFSF7 and TNFSF9 genes in 19p13.3 in diffuse large B-cell and Burkitt lymphomasRene Scholtysik
Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Essen, Germany
Int J Cancer 131:E830-5. 2012..Thus, TNFSF7 and TNFSF9 deletions are recurrent genetic lesions in multiple types of human lymphomas... - p53 protein expression in chronic lymphocytic leukemiaLudger Sellmann
Department of Hematology, Medical Faculty, University of Duisburg Essen, Essen, Germany
Leuk Lymphoma 53:1282-8. 2012..Taken together, the differential expression of p53 isoforms could disrupt the p53 response and contribute to CLL pathogenesis... - EBV transformation overrides gene expression patterns of B cell differentiation stagesDörte Siemer
University of Duisburg Essen, Medical School, Institute for Cell Biology Tumor Research, Virchowst 173, 45122 Essen, Germany
Mol Immunol 45:3133-41. 2008..Thus, EBV-infection deregulates multiple differentiation factors and processes in B cells, leading to a largely homogenous phenotype of EBV-infected B cells in latency III... - Molecular analysis of IgD-positive human germinal centresClaudia Müller
Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Virchowstrasse 173, 45122 Essen, Germany
Int Immunol 22:289-98. 2010.... - TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphomaRoland Schmitz
Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, 45122 Essen, Germany
J Exp Med 206:981-9. 2009..The significantly higher frequency of TNFAIP3 mutations in EBV(-) than EBV(+) cHL suggests complementing functions of TNFAIP3 inactivation and EBV infection in cHL pathogenesis... - Cellular origin and pathophysiology of chronic lymphocytic leukemiaMarc Seifert
Institute of Cell Biology Cancer Research, University of Duisburg Essen, Essen 45122, Germany
J Exp Med 209:2183-98. 2012..Finally, we identified deregulated proteins, including EBF1 and KLF transcription factors, that were not detected in previous comparisons of CLL and conventional B cells... 
Absence of measles virus genome and transcripts in Hodgkin-Reed/Sternberg cells of a cohort of Hodgkin lymphoma patientsEwerton Maggio
Institute for Cell Biology Tumor Research, University of Duisburg Essen, Essen, Germany
Int J Cancer 121:448-53. 2007..In this cohort of German and Israeli HL patients--including cases previously typed as MV-positive--and HL-derived cell lines, there was no evidence of MV genome in the HRS cells...- Transformation of BCR-deficient germinal-center B cells by EBV supports a major role of the virus in the pathogenesis of Hodgkin and posttransplantation lymphomasDörte Bechtel
Institute for Cell Biology Tumor Research, University of Duisburg Essen, Virchowstr 173, 45122 Essen, Germany
Blood 106:4345-50. 2005..The monoclonal LCLs represent valuable models for early stages of lymphoma development in classic HL and PTLD... - Molecular single-cell PCR analysis of rearranged immunoglobulin genes as a tool to determine the clonal composition of normal and malignant human B cellsRalf Kuppers
Tumor Research, Institute for Cell Biology, University of Essen Medical School, Essen, Germany
Methods Mol Biol 271:225-38. 2004..As a large part of the V-gene rearrangements is amplified, the approach is also useful to address additional issues, such as V-, D-, and J-gene usage and the presence and pattern of somatic mutations... - Advances in biology, diagnostics, and treatment of Hodgkin's diseaseRalf Kuppers
Institute for Cell Biology, Tumor Research, University of Duisburg Essen, Germany
Biol Blood Marrow Transplant 12:66-76. 2006.... - Origin and pathogenesis of nodular lymphocyte-predominant Hodgkin lymphoma as revealed by global gene expression analysisVerena Brune
Institute for Cell Biology Tumor Research, University of Duisburg Essen Medical School, 45122 Essen, Germany
J Exp Med 205:2251-68. 2008..Thus, these findings shed new light on the nature of L&H cells, reveal several novel pathogenetic mechanisms in NLPHL, and may help in differential diagnosis and lead to novel therapeutic strategies... - Telomeres and prognosis in patients with chronic lymphocytic leukaemiaLudger Sellmann
Department of Haematology, University of Duisburg Essen, Essen, Germany
Int J Hematol 93:74-82. 2011..Short telomeres and high telomerase activity as occurs in some patients with CLL with a worse prognosis might be an ideal target for treatment with telomerase inhibitors... - Molecular footprints of a germinal center derivation of human IgM+(IgD+)CD27+ B cells and the dynamics of memory B cell generationMarc Seifert
Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, D 45122 Essen, Germany
J Exp Med 206:2659-69. 2009..This provides novel insights into the dynamics of GC reactions and the generation of a memory B cell repertoire... - The Hodgkin and Reed/Sternberg cellRalf Kuppers
Institute for Cell Biology Tumor Research, University of Duisburg Essen, Medical School, Virchowstr 173, 45122 Essen, Germany
Int J Biochem Cell Biol 37:511-7. 2005..HRS or HRS-like cells are also found in several other diseases, e.g. as rare intermingled cells in some non-Hodgkin lymphomas and in infectious mononucleosis... - Prognosis in follicular lymphoma--it's in the microenvironmentRalf Kuppers
Institute for Cell Biology Tumor Research, University of Duisburg Essen Medical School, Essen, Germany
N Engl J Med 351:2152-3. 2004 - A model for the development of human IgD-only B cells: Genotypic analyses suggest their generation in superantigen driven immune responsesMarc Seifert
Institute for Cell Biology Tumor Research, University of Duisburg Essen, Medical School, Virchowstr 173, 45122 Essen, Germany
Mol Immunol 46:630-9. 2009..IgD-only B cells may hence represent a unique B lineage subset generated in response to particular antigens... - Reprogramming of the tumour B-cell phenotype in Hodgkin lymphomaRalf Kuppers
Institute for Cell Biology Tumor Research, University of Duisburg Essen, Medical School, 45122 Essen, Germany
Trends Immunol 27:203-5. 2006.... - Gene dosage effects in chronic lymphocytic leukemiaLudger Sellmann
Department of Hematology, Medical Faculty, University of Duisburg Essen, Hufelandstrase 55, 45122 Essen, Germany
Cancer Genet Cytogenet 203:149-60. 2010..We did not confirm a consistent correlation between MIRN15A and MIRN16-1 expression levels and BCL2 protein levels, indicating a complex regulation of BCL2 expression... - Trisomy 19 is associated with trisomy 12 and mutated IGHV genes in B-chronic lymphocytic leukaemiaLudger Sellmann
Department of Haematology, Medical Faculty, University of Duisburg Essen, Essen, Germany
Br J Haematol 138:217-20. 2007..Karyotypes of the present study and the literature identified a strong correlation to trisomy 18 in addition to trisomy 12. Trisomy 19 seems to be a secondary event in B-CLL with trisomy 12, mostly originating from mutated B cells... - Prognostic significance of Epstein-Barr virus-infected cells in the bone marrow of patients with nasal natural killer/T cell lymphomaRalf Kuppers
Institute for Cell Biology, Tumor Research, University of Duisburg Essen, Medical School, Essen, Germany
Haematologica 90:1011A. 2005 - Mutation analysis of the TNFAIP3 (A20) tumor suppressor gene in CLLClaudia Philipp
Institute of Cell Biology, University of Duisburg Essen, Medical School, Essen, Germany
Int J Cancer 128:1747-50. 2011..Therefore, A20 inactivation plays no significant role in the pathogenesis of CLL, and the recurrent deletion in CLL on 6q21-23, where TNFAIP3 is located, likely affects other gene(s)... - The major subtypes of human B-cell lymphomas lack mutations in BCL-2 family member BADRoland Schmitz
Institute for Cell Biology Tumor Research, University of Duisburg Essen, Medical School, Essen, Germany
Int J Cancer 119:1738-40. 2006..No mutations were found in any of the cases. We conclude that mutations in the BAD gene do not play a role in the pathogenesis of the major subtypes of human B-cell lymphomas... - Origin and pathogenesis of B cell lymphomasMarc Seifert
Institute of Cell Biology Cancer Research, Medical School, University of Duisburg Essen, Essen, Germany
Methods Mol Biol 971:1-25. 2013..Many B cell malignancies derive from germinal center B cells, most likely because of the high proliferation rate of these cells and the high activity of mutagenic processes... - Detection of genomic aberrations in molecularly defined Burkitt's lymphoma by array-based, high resolution, single nucleotide polymorphism analysisRene Scholtysik
Institute of Cell Biology Cancer Research, University of Duisburg Essen, Medical School, Virchowstr 173, 45122 Essen, Germany
Haematologica 95:2047-55. 2010..Knowledge about the genetic lesions that occur in Burkitt's lymphoma, besides the pathognomonic IG-MYC translocations, is limited... - Mechanisms of B-cell lymphoma pathogenesisRalf Kuppers
Institute for Cell Biology Tumor Research, University of Duisburg Essen, Medical School, Germany
Nat Rev Cancer 5:251-62. 2005..Recent insights into the lymphomagenic role of factors supplied by the microenvironment also offer new therapeutic strategies...