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Genomes and GenesSpecies | D KoeslingSummaryCountry: Germany Publications
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Negative feedback in NO/cGMP signallingD Koesling
Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, Ruhr Universitat Bochum, Med Fak MA N1, Universitatsstrasse 150, 44780 Bochum, Germany
Biochem Soc Trans 33:1119-22. 2005..As the cGMP-induced PDE5 activation turned out to be sustained, in the range of hours, it is probably responsible for the NO-induced desensitization observed within NO/cGMP signalling...- Nitric oxide-sensitive guanylyl cyclase: structure and regulationDoris Koesling
Institut fur Pharmakologie und Toxikologie, Medizinische Fakultät MA N1, Ruhr Universitat Bochum, 44780 Bochum, Germany
Neurochem Int 45:813-9. 2004..In sum, the two known isoforms may stand for the neuronal and vascular form of NO-sensitive GC reflecting a possible association to the neuronal and endothelial NO-synthase, respectively... - Regulation of nitric oxide-sensitive guanylyl cyclaseAndreas Friebe
Abteilung für Pharmakologie, Medizinische Fakultat, Ruhr Universitat Bochum, Universitatsstr 150, 44780 Bochum, Germany
Circ Res 93:96-105. 2003..Finally, regulation of GC expression and a possible modulation of GC activity by other factors are discussed... 
Nitric oxide-sensitive guanylyl cyclase is the only nitric oxide receptor mediating platelet inhibitionO Dangel
Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, Ruhr Universitat Bochum, Bochum, Germany
J Thromb Haemost 8:1343-52. 2010..However, NO has been postulated to influence these platelet functions also via cGMP-independent mechanisms...- Desensitization of NO/cGMP signaling in smooth muscle: blood vessels versus airwaysFlorian Mullershausen
, , , , 44780 Bochum, Germany
Mol Pharmacol 69:1969-74. 2006..In summary, our results demonstrate the physiological impact of PDE5 activation on the control of smooth muscle tone and provide an explanation for the apparent impairment of NO-induced vasorelaxation... - Exogenous nitric oxide donation causes desensitization of arteriolar relaxing activity in vivo: an intravital analysis in miceAndrej Ring
Department of Surgery, Trauma Center, University Hospital Bergmannsheil, Bochum, Germany
J Surg Res 164:169-74. 2010..To examine whether a similar phenomenon can be detected in vivo, we analyzed relaxations of microvessels in response to repeated applications of NO... 
Spare guanylyl cyclase NO receptors ensure high NO sensitivity in the vascular systemEvanthia Mergia
Institut fur Pharmakologie und Toxikologie, Ruhr Universitat Bochum, Bochum, Germany
J Clin Invest 116:1731-7. 2006....- In vivo reconstitution of the negative feedback in nitric oxide/cGMP signaling: role of phosphodiesterase type 5 phosphorylationFlorian Mullershausen
Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, Ruhr Universitat Bochum, Bochum, Germany
Mol Biol Cell 15:4023-30. 2004..Phosphorylation of PDE5 seems to act as memory switch for activation leading to long-term desensitization of the signaling pathway... - The enhanced NO-induced cGMP response induced by long-term L-NAME treatment is not due to enhanced expression of NO-sensitive guanylyl cyclaseFlorian Mullershausen
Pharmakologie und Toxikologie, Medizinische Fakultät MA N1 39, Ruhr Universitat Bochum, Universitatsstr 150, 44780 Bochum, Germany
Vascul Pharmacol 40:161-5. 2003..The results show that a reduction of the endogenous NO production induced by long-term L-NAME treatment does not lead to an up-regulation of NO-sensitive GC on the level of protein expression... - Direct activation of PDE5 by cGMP: long-term effects within NO/cGMP signalingFlorian Mullershausen
Abteilung für Pharmakologie und Toxikologie, Medizinische Fakultat, Ruhr Universitat Bochum, MA N1 43, Universitaetsstr 150, 44780 Bochum, Germany
J Cell Biol 160:719-27. 2003..In sum, we suggest NO-induced activation and phosphorylation of PDE5 as the mechanism for a long-lasting negative feedback loop shaping the cGMP response in human platelets in order to adapt to the amount of NO available... - More than a retrograde messenger: nitric oxide needs two cGMP pathways to induce hippocampal long-term potentiationFeras Taqatqeh
Department of Neurophysiology, Medical School, Ruhr University Bochum, 44780 Bochum, Germany
J Neurosci 29:9344-50. 2009..The unexpected role of cGMP at two sites of the synaptic cleft explains many of the controversial results in former NO research in LTP and demonstrates the necessity of presynaptic and postsynaptic changes for LTP expression... - Guanylyl cyclase/PSD-95 interaction: targeting of the nitric oxide-sensitive alpha2beta1 guanylyl cyclase to synaptic membranesM Russwurm
Pharmakologie und Toxikologie, Medizinische Fakultät MA N1, Ruhr Universitat Bochum, 44780 Bochum, Germany
J Biol Chem 276:44647-52. 2001.... - Isoforms of NO-sensitive guanylyl cyclaseMichael Russwurm
Pharmakologie und Toxikologie, Medizinische Fakultät MA N1, Ruhr Universitat Bochum, Germany
Mol Cell Biochem 230:159-64. 2002..The interaction with PSD-95 locates the alpha2beta1 isoform in close proximity to the NO-generating NO synthase thereby enabling the NO sensor to respond to locally raised NO concentrations... - Inhibition of deactivation of NO-sensitive guanylyl cyclase accounts for the sensitizing effect of YC-1Michael Russwurm
Pharmakologie und Toxikologie, , , Germany
J Biol Chem 277:24883-8. 2002..In contrast to the wild type enzyme, YC-1 did not increase NO sensitivity of this mutant, clearly establishing inhibition of deactivation as the underlying mechanism of the NO sensitizer YC-1... - Fatal gastrointestinal obstruction and hypertension in mice lacking nitric oxide-sensitive guanylyl cyclaseAndreas Friebe
Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, Ruhr Universitat Bochum, 44780 Bochum, Germany
Proc Natl Acad Sci U S A 104:7699-704. 2007..Our knockout model completely disrupts the NO/cGMP signaling cascade and provides evidence for the unique role of NO-GC as NO receptor... - Dimerization of nitric oxide-sensitive guanylyl cyclase requires the alpha 1 N terminusCorina Wagner
Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, MA N1, Ruhr Universitat Bochum, 44780 Bochum, Germany
J Biol Chem 280:17687-93. 2005..Whereas beta(1) forms heme containing homodimers and is stable, alpha(1) appears to be prone to misfolding and degradation when heterodimerization is impaired by deletion of important sequences... - Purification and characterization of NO-sensitive guanylyl cyclaseMichael Russwurm
, , Germany
Methods Enzymol 396:492-501. 2005..In this chapter, a fast method for purification of the enzyme from bovine lungs is described, and basic methods for characterization of the enzyme are summarized... - Dual acylation of PDE2A splice variant 3: targeting to synaptic membranesCorina Russwurm
Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, Ruhr Universitat Bochum, Bochum 44780, Germany
J Biol Chem 284:25782-90. 2009..In sum, these results demonstrate dual acylation as mechanism targeting neuronal PDE2A3 to synapses thereby ensuring local control of cyclic nucleotides... - Design of fluorescence resonance energy transfer (FRET)-based cGMP indicators: a systematic approachMichael Russwurm
Institut fur Pharmakologie und Toxikologie, Ruhr Universitat Bochum, Universitatsstrasse 150, 44780 Bochum, Federal Republic of Germany
Biochem J 407:69-77. 2007..The in vivo performance of these new indicators is demonstrated in living cells and validated by comparison with cGMP dynamics as measured by radioimmunoassays... - cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase IAchim Schmidtko
Pharmazentrum frankfurt ZAFES, Institut fur Klinische Pharmakologie, Klinikum der Johann Wolfgang Goethe Universitat, 60590 Frankfurt am Main, Germany
J Neurosci 28:8568-76. 2008..Together, our results provide evidence that NO-GC is crucially involved in the central sensitization of pain pathways during inflammatory and neuropathic pain... - Effects of nitroglycerin/L-cysteine on soluble guanylate cyclase: evidence for an activation/inactivation equilibrium controlled by nitric oxide binding and haem oxidationAntonius C F Gorren
Department of Pharmacology and Toxicology, , A-8010 Graz, Austria
Biochem J 390:625-31. 2005..These results suggest that the effect of GTN/Cys is the result of an activation/inactivation equilibrium that is controlled by the rate of NO release and haem oxidation... - Long-term potentiation in the visual cortex requires both nitric oxide receptor guanylyl cyclasesArash Haghikia
Faculty of Medicine, Institute of Physiology, Department of Neurophysiology, Ruhr University Bochum, D 44780 Bochum, Germany
J Neurosci 27:818-23. 2007..The requirement of both NO receptor GCs for LTP indicates the existence of two distinct NO/cGMP-mediated pathways, which have to work in concert for expression of LTP... - Major occurrence of the new alpha2beta1 isoform of NO-sensitive guanylyl cyclase in brainEvanthia Mergia
Pharmakologie und Toxikologie, , , 44780, Bochum, Germany
Cell Signal 15:189-95. 2003..In sum, the major occurrence of the alpha(2)beta(1) heterodimer suggests a special role in synaptic transmission; whether this isoform outside the brain also occurs in neuronal networks has to be addressed in future studies... - Inhibition of phosphodiesterase type 5 by the activator of nitric oxide-sensitive guanylyl cyclase BAY 41-2272Florian Mullershausen
, , Bochum, Germany
Circulation 109:1711-3. 2004..CONCLUSIONS: The physiological effects of BAY 41-2272, which are commonly ascribed to the NO-independent activation of NO-sensitive GC, are rather due to the synergism of sensitization of NO-sensitive GC and inhibition of PDE5... - Contribution of aldehyde dehydrogenase to mitochondrial bioactivation of nitroglycerin: evidence for the activation of purified soluble guanylate cyclase through direct formation of nitric oxideAlexander Kollau
Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
Biochem J 385:769-77. 2005..In addition, the previous conflicting notion of the existence of a high-affinity GTN-metabolizing pathway operating in intact blood vessels but not in tissue homogenates is explained... - NO activation of guanylyl cyclaseMichael Russwurm
Institute for Pharmacology und Toxicology, Medical Faculty, Ruhr-University Bochum, Bochum, Germany
EMBO J 23:4443-50. 2004..The model not only covers the data reported here but also consolidates results from previous studies on NO-binding and dissociation/deactivation of GC... - NO-sensitive guanylyl cyclase and NO-induced feedback inhibition in cGMP signalingFlorian Mullershausen
Institut fuer Pharmakologie und Toxikologie, Medizinische Fakultaet MA N1, Ruhr-Universitaet Bochum, Universitaetsstr 150, 44789 Bochum, Germany
Front Biosci 10:1269-78. 2005..Moreover, tuning of PDE5 activity may also represent a regulatory link to mediate cross talk between NO-induced and natriuretic peptide-induced cGMP signaling in general... - Bioactivation of nitroglycerin by purified mitochondrial and cytosolic aldehyde dehydrogenasesMatteo Beretta
Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
J Biol Chem 283:17873-80. 2008..Incubation of ALDH1 or ALDH2 with GTN resulted in sustained, chloral hydrate-sensitive formation of NO. These data may explain the coupling of ALDH2-catalyzed GTN metabolism to sGC activation in vascular smooth muscle... - Bioactivation of nitroglycerin by ascorbateAlexander Kollau
Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
Mol Pharmacol 72:191-6. 2007..This reaction may contribute to GTN bioactivation in blood vessels under conditions of GTN tolerance and ascorbate supplementation...