R Klopfleisch

Summary

Country: Germany

Publications

  1. doi Canine cutaneous peripheral nerve sheath tumours versus fibrosarcomas can be differentiated by neuroectodermal marker genes in their transcriptome
    R Klopfleisch
    Institute of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, Berlin, Germany
    J Comp Pathol 148:197-205. 2013
  2. doi Tandem duplication of KIT exon 11 influences the proteome of canine mast cell tumours
    P Schlieben
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163, Berlin, Germany
    J Comp Pathol 148:318-22. 2013
  3. doi The combined expression pattern of BMP2, LTBP4, and DERL1 discriminates malignant from benign canine mammary tumors
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, 14163, Berlin, Germany
    Vet Pathol 47:446-54. 2010
  4. doi The metastatic cascade is reflected in the transcriptome of metastatic canine mammary carcinomas
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, Berlin, Germany
    Vet J 190:236-43. 2011
  5. doi RAD51 protein expression is increased in canine mammary carcinomas
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, Berlin, Germany
    Vet Pathol 47:98-101. 2010
  6. doi Efficient and cost-effective extraction of genomic DNA from formalin-fixed and paraffin-embedded tissues
    A Th A Weiss
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Str 15, 14163 Berlin, Germany
    Vet Pathol 48:834-8. 2011
  7. doi Loss of p27 expression in canine mammary tumors and their metastases
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, 14163 Berlin, Germany
    Res Vet Sci 88:300-3. 2010
  8. doi Canine cutaneous histiocytomas are clonal lesions as defined by X-linked clonality testing
    N M Delcour
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Str 15, 14163 Berlin, Germany
    J Comp Pathol 149:192-8. 2013
  9. doi Increased Derlin-1 expression in metastases of canine mammary adenocarcinomas
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, Germany
    J Comp Pathol 142:79-83. 2010
  10. doi Multiple RT-PCR markers for the detection of circulating tumour cells of metastatic canine mammary tumours
    A Da Costa
    Institute of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163 Berlin, Germany
    Vet J 196:34-9. 2013

Collaborators

  • A D Gruber
  • A Meyer
  • A Th A Weiss
  • M Hummel
  • A Jansen
  • B Ehlers
  • A Da Costa
  • P Olias
  • B Kohn
  • P Schlieben
  • N M Delcour
  • M C von Deetzen
  • A Bondzio
  • C Weise
  • A Ochs
  • L Mundhenk
  • U Seelig
  • B Schmeck
  • M Bothe
  • D Lenze
  • R Einspanier
  • E Schulz
  • J T Oliveira
  • F Gartner
  • B Kaser-Hotz
  • L Brunnberg
  • K Steinicke
  • E Luge
  • F Lotz
  • G Arndt
  • K Nockler
  • B Walther
  • C Weingart
  • B Guerra
  • A Luebke-Becker

Detail Information

Publications33

  1. doi Canine cutaneous peripheral nerve sheath tumours versus fibrosarcomas can be differentiated by neuroectodermal marker genes in their transcriptome
    R Klopfleisch
    Institute of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, Berlin, Germany
    J Comp Pathol 148:197-205. 2013
    ..Furthermore, the genes associated with the fibrosarcoma phenotype may serve as markers to differentiate fibrosarcomas from other tumour types...
  2. doi Tandem duplication of KIT exon 11 influences the proteome of canine mast cell tumours
    P Schlieben
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163, Berlin, Germany
    J Comp Pathol 148:318-22. 2013
    ....
  3. doi The combined expression pattern of BMP2, LTBP4, and DERL1 discriminates malignant from benign canine mammary tumors
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, 14163, Berlin, Germany
    Vet Pathol 47:446-54. 2010
    ..This pilot study identified a complex mRNA expression pattern of 3 genes that was able to identify malignancy in laser-microdissected tumor cells for each individual tumor, instead of group means as used in previous studies...
  4. doi The metastatic cascade is reflected in the transcriptome of metastatic canine mammary carcinomas
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, Berlin, Germany
    Vet J 190:236-43. 2011
    ..Gene expression profiling of metastatic carcinomas, therefore, identified molecular pathways and functional gene families that are deregulated during malignant progression in canine mammary tumors...
  5. doi RAD51 protein expression is increased in canine mammary carcinomas
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, Berlin, Germany
    Vet Pathol 47:98-101. 2010
    ..Nevertheless, the increased RAD51 mRNA expression in metastases could not be confirmed by immunohistochemistry...
  6. doi Efficient and cost-effective extraction of genomic DNA from formalin-fixed and paraffin-embedded tissues
    A Th A Weiss
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Str 15, 14163 Berlin, Germany
    Vet Pathol 48:834-8. 2011
    ..The protocol allows stable amplification of DNA fragments up to 600 bp in length in a wide variety of tissues...
  7. doi Loss of p27 expression in canine mammary tumors and their metastases
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, 14163 Berlin, Germany
    Res Vet Sci 88:300-3. 2010
    ....
  8. doi Canine cutaneous histiocytomas are clonal lesions as defined by X-linked clonality testing
    N M Delcour
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Str 15, 14163 Berlin, Germany
    J Comp Pathol 149:192-8. 2013
    ..Analysis of 11 histiocytomas identified a non-random X chromosome inactivation of one androgen receptor allele. CCH may, therefore, be a clonal lesion and of neoplastic nature. ..
  9. doi Increased Derlin-1 expression in metastases of canine mammary adenocarcinomas
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, Germany
    J Comp Pathol 142:79-83. 2010
    ..Weak Derl-1 protein expression was also found in vascular endothelial cells and fibrocytes and occasionally in lymphatic lining cells...
  10. doi Multiple RT-PCR markers for the detection of circulating tumour cells of metastatic canine mammary tumours
    A Da Costa
    Institute of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163 Berlin, Germany
    Vet J 196:34-9. 2013
    ..However, a multimarker assay of four genes significantly enhanced the sensitivity of the assay and is therefore preferable for CTC detection...
  11. doi Iron overload syndrome in the black rhinoceros (Diceros bicornis): microscopical lesions and comparison with other rhinoceros species
    P Olias
    Department of Veterinary Pathology, Faculty of Veterinary Medicine, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163 Berlin, Germany
    J Comp Pathol 147:542-9. 2012
    ....
  12. doi Differences in the proteome of high-grade versus low-grade canine cutaneous mast cell tumours
    P Schlieben
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163 Berlin, Germany
    Vet J 194:210-4. 2012
    ..It is noteworthy that some of the proteins identified have been proposed as therapeutic targets for human oncology and it will be interesting to evaluate their therapeutic and diagnostic potential for canine MCTs...
  13. doi Insulin receptor is expressed in normal canine mammary gland and benign adenomas but decreased in metastatic canine mammary carcinomas similar to human breast cancer
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, Berlin, Germany
    Vet Comp Oncol 8:293-301. 2010
    ..However, decreased INSR expression carcinomas and their metastases render questionable its impact at late stages of carcinogenesis...
  14. doi Potential markers for detection of circulating canine mammary tumor cells in the peripheral blood
    A Da Costa
    Institute of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, Berlin, Germany
    Vet J 190:165-8. 2011
    ..These CTC markers are thus candidate markers for identifying canine mammary CTC in the peripheral blood and may serve as prognostic factors for metastatic behavior in the future...
  15. doi Identification of six potential markers for the detection of circulating canine mammary tumour cells in the peripheral blood identified by microarray analysis
    A Da Costa
    Institute of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, 14163 Berlin, Germany
    J Comp Pathol 146:143-51. 2012
    ..The six mRNA markers may be suitable for detection of canine mammary CTCs and allow the analysis of their spatiotemporal distribution in dogs with mammary tumours...
  16. doi All subunits of the interleukin-2 receptor are expressed by canine cutaneous mast cell tumours
    A Meyer
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163 Berlin, Germany
    J Comp Pathol 149:19-29. 2013
    ..The decrease in IL-2R expression with increasing histological evidence of malignancy suggests that the IL-2R may be more relevant for early MCT development and well-differentiated tumours...
  17. doi Derlin-1 and stanniocalcin-1 are differentially regulated in metastasizing canine mammary adenocarcinomas
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, 14163 Berlin, Germany
    J Comp Pathol 141:113-20. 2009
    ..Taken together, these results suggest that malignant behaviour of canine mammary adenocarcinoma is associated with reduced transcription of the stanniocalcin-1 gene and overexpression of the derlin-1 gene...
  18. doi Increased expression of BRCA2 and RAD51 in lymph node metastases of canine mammary adenocarcinomas
    R Klopfleisch
    Institute of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strasse 15, 14163 Berlin, Germany
    Vet Pathol 46:416-22. 2009
    ..Further experimental studies are needed to clarify whether these changes in gene expression are a direct carcinogenetic stimulus or a protective response due to genetic instability during tumor progression...
  19. doi Molecular quantification of canine specific microRNA species
    M C von Deetzen
    Institute of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertagstraße 15, 14163 Berlin, Germany
    Res Vet Sci 95:562-8. 2013
    ..GeNorm and NormFinder analysis were used for the evaluation of potential housekeeping genes and identified miRNAs to be more stable housekeeping genes by trend. ..
  20. doi The pathology of comparative animal models of human haemochromatosis
    R Klopfleisch
    Department of Veterinary Pathology, Faculty of Veterinary Medicine, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163 Berlin, Germany
    J Comp Pathol 147:460-78. 2012
    ....
  21. doi Downregulation of transforming growth factor β (TGFβ) and latent TGFβ binding protein (LTBP)-4 expression in late stage canine mammary tumours
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Berlin, Germany
    Vet J 186:379-84. 2010
    ....
  22. doi Iron storage disease in red deer (Cervus elaphus elaphus) is not associated with mutations in the HFE gene
    P Olias
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163 Berlin, Germany
    J Comp Pathol 145:207-13. 2011
    ....
  23. pmc An emerging pulmonary haemorrhagic syndrome in dogs: similar to the human leptospiral pulmonary haemorrhagic syndrome?
    R Klopfleisch
    Institute of Veterinary Pathology, Department of Veterinary Medicine, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163 Berlin, Germany
    Vet Med Int 2010:928541. 2010
    ..The lack of a consistent post mortem identification of Leptospira spp. in dogs with pulmonary haemorrhage raise questions as to whether additional factors besides Leptospira may cause this as yet unrecognized entity in dogs...
  24. doi Septic polyarthritis caused by Erysipelothrix rhusiopathiae in a dog
    U Seelig
    Clinic for Small Animals, Freie Universitat Berlin, College of Veterinary Medicine, Berlin, Germany
    Vet Comp Orthop Traumatol 23:71-3. 2010
    ..The radiographical, bacteriological and necropsy findings confirmed a diagnosis of septic polyarthritis due to infection with Erysipelothrix rhusiopathiae...
  25. doi Pulmonary abnormalities in dogs with leptospirosis
    B Kohn
    Small Animal Clinic, Faculty of Veterinary Medicine, Freie Universitat Berlin, Berlin, Germany
    J Vet Intern Med 24:1277-82. 2010
    ..Leptospirosis in dogs is a multiorgan disease affecting mostly kidneys and liver...
  26. doi T-cell receptor γ chain variable and joining region genes of subgroup 1 are clonally rearranged in feline B- and T-cell lymphoma
    A Th A Weiss
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Str 15, 14163 Berlin, Germany
    J Comp Pathol 144:123-34. 2011
    ..2, 1.3 and 1.5. V region variants 2 and 4 were not identified in either tumour type. Some feline B-cell lymphomas had a clonally rearranged TCR, a finding reported in human, but not canine, B-cell lymphoma...
  27. doi Differential expression of cell cycle regulators p21, p27 and p53 in metastasizing canine mammary adenocarcinomas versus normal mammary glands
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Strabe 15, 14163 Berlin, Germany
    Res Vet Sci 87:91-6. 2009
    ..Taken together the results suggest that loss of p21 overexpression is associated with tumor metastasis while reduced cell cycle inhibition by p27 is associated with malignant progression...
  28. doi Metastatic endocervical adenocarcinoma in a western lowland gorilla (Gorilla g. gorilla): no evidence of virus-induced carcinogenesis
    P Olias
    Department of Veterinary Pathology, Faculty of Veterinary Medicine, Freie Universitat Berlin, Berlin, Germany
    J Med Primatol 41:142-6. 2012
    ..Nevertheless, similar tumours have only been rarely described in Great Apes. This report characterizes the pathological and molecular features of a metastatic endocervical adenocarcinoma in a Western lowland gorilla (Gorilla g. gorilla)...
  29. doi Does the taking of biopsies affect the metastatic potential of tumours? A systematic review of reports on veterinary and human cases and animal models
    R Klopfleisch
    Freie Universitat Berlin, Institute of Veterinary Pathology, 14163 Berlin, Germany
    Vet J 190:e31-42. 2011
    ..The overall risk therefore appears to be negligible when compared to the valuable information obtained from biopsies in veterinary practice...
  30. doi CD25 is expressed by canine cutaneous mast cell tumors but not by cutaneous connective tissue mast cells
    A Meyer
    Department of Veterinary Pathology, College of Veterinary Medicine, Freie Universitat Berlin, Robert von Ostertag Strasse 15, 14163 Berlin, Germany
    Vet Pathol 49:988-97. 2012
    ..Because of the low number of CD25-positive tumor cells in high-grade tumors, the usefulness of CD25 as a tumor marker is, however, questionable...
  31. doi Multiple Polymerase Chain Reaction Markers for the Differentiation of Canine Cutaneous Peripheral Nerve Sheath Tumours versus Canine Fibrosarcomas
    A Meyer
    Institute of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, 14163 Berlin, Germany
    J Comp Pathol 150:198-203. 2014
    ....
  32. ncbi Excavation of a buried treasure--DNA, mRNA, miRNA and protein analysis in formalin fixed, paraffin embedded tissues
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, Berlin, Germany
    Histol Histopathol 26:797-810. 2011
    ..In addition, methods and general problems of DNA, miRNA, mRNA and protein extraction and the current knowledge on the feasibility and accuracy of quantitative gene expression analysis in FFPE tissues is summarized...
  33. doi Weigners fixative-an alternative to formalin fixation for histology with improved preservation of nucleic acids
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Berlin, Germany
    Vet Pathol 50:191-9. 2013
    ..Weigners may therefore be a promising alternative to cryopreservation and may be embraced by people affected by formalin allergies...