D Kloor

Summary

Country: Germany

Publications

  1. ncbi request reprint Effects of ions on adenosine binding and enzyme activity of purified S-adenosylhomocysteine hydrolase from bovine kidney
    D Kloor
    Department of Pharmacology, University of Tubingen, Germany
    Biochem Pharmacol 56:1493-6. 1998
  2. ncbi request reprint Expression and localization of S-adenosylhomocysteine-hydrolase in the rat kidney following carbon monoxide induced hypoxia
    Doris Kloor
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 19:57-66. 2007
  3. ncbi request reprint Studies of S-adenosylhomocysteine-hydrolase polymorphism in a Croatian population
    Doris Kloor
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, 72074 Tubingen, Germany
    J Hum Genet 51:21-4. 2006
  4. ncbi request reprint Characterization of glycine N-methyltransferase from rabbit liver
    Doris Kloor
    Department of Pharmacology and Toxicology, Wilhelmstrasse 56, Faculty of Medicine, Univesrity of Tübingen, D 72074 Tubingen, Germany
    Biochem Cell Biol 82:369-74. 2004
  5. ncbi request reprint S-Adenosylhomocysteine hydrolase as a target for intracellular adenosine action
    Doris Kloor
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074 Tubingen, Germany
    Trends Pharmacol Sci 25:294-7. 2004
  6. ncbi request reprint Adenosine binding sites at S-adenosylhomocysteine hydrolase are controlled by the NAD+/NADH ratio of the enzyme
    Doris Kloor
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074 Tuebingen, Germany
    Biochem Pharmacol 66:2117-23. 2003
  7. doi request reprint Determinants for the cAMP-binding site at the S-adenosylhomocysteine-hydrolase
    Doris Kloor
    Department of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, Tubingen, Germany
    Naunyn Schmiedebergs Arch Pharmacol 380:215-22. 2009
  8. ncbi request reprint Characterization of the cAMP binding site of purified S-adenosyl-homocysteine hydrolase from bovine kidney
    Doris Kloor
    Department of Pharmacology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074 Tubingen, Germany
    Biochem Pharmacol 64:1201-6. 2002
  9. ncbi request reprint Tissue levels of S-adenosylhomocysteine in the rat kidney: effects of ischemia and homocysteine
    Doris Kloor
    Department of Pharmacology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074, Tubingen, Germany
    Biochem Pharmacol 63:809-15. 2002
  10. ncbi request reprint Simple and sensitive binding assay for measurement of adenosine using reduced S-adenosylhomocysteine hydrolase
    D Kloor
    Department of Pharmacology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074 Tubingen, Germany
    Clin Chem 46:537-42. 2000

Collaborators

Detail Information

Publications23

  1. ncbi request reprint Effects of ions on adenosine binding and enzyme activity of purified S-adenosylhomocysteine hydrolase from bovine kidney
    D Kloor
    Department of Pharmacology, University of Tubingen, Germany
    Biochem Pharmacol 56:1493-6. 1998
    ..These findings imply that SAH-sensitive transmethylation reactions are inhibited during renal hypoxia when intracellular levels of phosphate, adenosine, and SAH are elevated...
  2. ncbi request reprint Expression and localization of S-adenosylhomocysteine-hydrolase in the rat kidney following carbon monoxide induced hypoxia
    Doris Kloor
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Tubingen, Germany
    Cell Physiol Biochem 19:57-66. 2007
    ....
  3. ncbi request reprint Studies of S-adenosylhomocysteine-hydrolase polymorphism in a Croatian population
    Doris Kloor
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, 72074 Tubingen, Germany
    J Hum Genet 51:21-4. 2006
    ..Moreover, a total of four different electromorphic SAHHs have been observed, giving allele frequencies calculated as SAHH 1 = 0.941, SAHH 2 = 0.032, SAHH 3 = 0.006, SAHH 4 = 0.015, and SAHH 0 = 0.006...
  4. ncbi request reprint Characterization of glycine N-methyltransferase from rabbit liver
    Doris Kloor
    Department of Pharmacology and Toxicology, Wilhelmstrasse 56, Faculty of Medicine, Univesrity of Tübingen, D 72074 Tubingen, Germany
    Biochem Cell Biol 82:369-74. 2004
    ..Based on the kinetic data glycine N-methyltransferase from rabbit liver exhibits appreciable activity at physiological S-adenosylmethionine and S-adenosylhomocysteine levels...
  5. ncbi request reprint S-Adenosylhomocysteine hydrolase as a target for intracellular adenosine action
    Doris Kloor
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074 Tubingen, Germany
    Trends Pharmacol Sci 25:294-7. 2004
    ..Thus, the ratio between adenosine and cAMP, which can vary under different physiological conditions, might result in changes in, for example, DNA promoter methylation and therefore transcription...
  6. ncbi request reprint Adenosine binding sites at S-adenosylhomocysteine hydrolase are controlled by the NAD+/NADH ratio of the enzyme
    Doris Kloor
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074 Tuebingen, Germany
    Biochem Pharmacol 66:2117-23. 2003
    ..In conclusion, our data show that AdoHcy hydrolase has two different Ado binding sites which are dependent upon the enzyme-bound NAD(+)/NADH ratios...
  7. doi request reprint Determinants for the cAMP-binding site at the S-adenosylhomocysteine-hydrolase
    Doris Kloor
    Department of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, Tubingen, Germany
    Naunyn Schmiedebergs Arch Pharmacol 380:215-22. 2009
    ..Moreover, cAMP did not interact with the catalytic site of AdoHcy-hydrolase and did not act as an allosteric effector for the AdoHcy-hydrolase...
  8. ncbi request reprint Characterization of the cAMP binding site of purified S-adenosyl-homocysteine hydrolase from bovine kidney
    Doris Kloor
    Department of Pharmacology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074 Tubingen, Germany
    Biochem Pharmacol 64:1201-6. 2002
    ..Our data indicate that the binding site for nanomolar concentrations of cAMP and adenosine at the AdoHcyase appears to be identical. The physiological implications of a cAMP binding site at the AdoHcyase remain to be established...
  9. ncbi request reprint Tissue levels of S-adenosylhomocysteine in the rat kidney: effects of ischemia and homocysteine
    Doris Kloor
    Department of Pharmacology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074, Tubingen, Germany
    Biochem Pharmacol 63:809-15. 2002
    ....
  10. ncbi request reprint Simple and sensitive binding assay for measurement of adenosine using reduced S-adenosylhomocysteine hydrolase
    D Kloor
    Department of Pharmacology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074 Tubingen, Germany
    Clin Chem 46:537-42. 2000
    ..We developed a simple and sensitive binding assay for the detection of adenosine based on the displacement of [(3)H]adenosine from S-adenosylhomocysteine (SAH) hydrolase in its reduced form...
  11. ncbi request reprint Adenosine metabolism and its effect on methylation potential in cultured cells: methodological considerations
    M Hermes
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tuebingen, Wilhelmstrasse 56, D 72074, Tuebingen, Germany
    Cell Mol Biol (Noisy-le-grand) 52:OL874-81. 2006
    ..In conclusion our data demonstrate that extracellular adenosine concentration and intracellular metabolite concentration strongly depend on the methods used to culture and harvest the cells...
  12. ncbi request reprint Effect of acute hyperhomocysteinemia on methylation potential of erythrocytes and on DNA methylation of lymphocytes in healthy male volunteers
    R Fux
    Dept of Pharmacology and Toxicology, Div of Experimental Pharmacology, Univ Hospital Tübingen, Wilhelmstr 56, D 72074 Tubingen, Germany
    Am J Physiol Renal Physiol 289:F786-92. 2005
    ..Although chronic elevation of tHcy has been shown to affect MP and DNA methylation, acute elevation of plasma tHcy above 20 micromol/l for 8 h is not sufficient to change MP and to induce DNA hypomethylation in lymphocytes...
  13. ncbi request reprint Dopamine D(3) receptors and salt-dependent hypertension
    G Luippold
    Department of Pharmacology, University of Tubingen, Tubingen, Germany
    J Am Soc Nephrol 12:2272-9. 2001
    ....
  14. doi request reprint S-Adenosylhomocysteine hydrolase overexpression in HEK-293 cells: effect on intracellular adenosine levels, cell viability, and DNA methylation
    Marina Hermes
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tuebingen, Germany
    Cell Physiol Biochem 22:223-36. 2008
    ....
  15. ncbi request reprint Lack of effect of extracellular adenosine generation and signaling on renal erythropoietin secretion during hypoxia
    Almut Grenz
    Dept of Pharmacology and Toxicology, Tübingen Univ Hospital, Wilhelmstr 56, D 72074 Tubingen, Germany
    Am J Physiol Renal Physiol 293:F1501-11. 2007
    ..Together, these studies combine genetic and pharmacological in vivo evidence that increases of EPO secretion during limited oxygen availability are not affected by extracellular adenosine generation or signaling...
  16. ncbi request reprint Protective role of ecto-5'-nucleotidase (CD73) in renal ischemia
    Almut Grenz
    Department of Pharmacology and Toxicology, Tubingen University Hospital, Tubingen, Germany
    J Am Soc Nephrol 18:833-45. 2007
    ....
  17. ncbi request reprint Role of S-adenosylhomocysteine hydrolase in adenosine-induced apoptosis in HepG2 cells
    Marina Hermes
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tuebingen, Wilhelmstrasse 56, D 72074 Tuebingen, Germany
    Exp Cell Res 313:264-83. 2007
    ..Since several p53-target genes and other apoptosis-related genes were up-regulated by Adox, we conclude that AdoHcy is involved in adenosine-induced apoptosis by altering gene expression...
  18. ncbi request reprint S-adenosylhomocysteine metabolism in different cell lines: effect of hypoxia and cell density
    Marina Hermes
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Germany
    Cell Physiol Biochem 15:233-44. 2005
    ..Exp Cell Res 294: 325-334, 2004). In the present study, we compared AdoMet/AdoHcy ratio and energy metabolism in HepG2, HEK-293, HeLa, MCF-7 and SK-HEP-1 cell lines under normoxia and hypoxia...
  19. ncbi request reprint Influence of an altered methylation potential on mRNA methylation and gene expression in HepG2 cells
    Marina Hermes
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, D 72074 Tubingen, Germany
    Exp Cell Res 294:325-34. 2004
    ..Our results show that the mRNA levels of the studied genes respond differentially to changes in MP. This implies that genes with a slower transcription rate and mRNAs with a slower turnover are insensitive to short-term changes in MP...
  20. ncbi request reprint Functional analysis of human S-adenosylhomocysteine hydrolase isoforms SAHH-2 and SAHH-3
    Ksenija Fumic
    Clinical Institute of Laboratory Diagnosis, University Hospital Center, Zagreb, Croatia
    Eur J Hum Genet 15:347-51. 2007
    ....
  21. pmc Role of extracellular nucleotide phosphohydrolysis in intestinal ischemia-reperfusion injury
    Melanie L Hart
    Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    FASEB J 22:2784-97. 2008
    ..These data reveal a previously unrecognized role of CD73 in attenuating intestinal IR-mediated injury...
  22. doi request reprint Alterations in S-adenosylhomocysteine metabolism decrease O6-methylguanine DNA methyltransferase gene expression without affecting promoter methylation
    Marina Hermes
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Tubingen, Wilhelmstrasse 56, D 72074 Tubingen, Germany
    Biochem Pharmacol 75:2100-11. 2008
    ..Thus, the present study suggests elevation of AdoHcy levels by AdoHcy hydrolase inhibition as a novel pharmacological approach to modulate MGMT expression and to increase the responsiveness to alkylating agents...
  23. doi request reprint Direct treatment of mouse or human blood with soluble 5'-nucleotidase inhibits platelet aggregation
    Melanie L Hart
    Department of Anesthesiology and Intensive Care Medicine, University of Tubingen, Germany
    Arterioscler Thromb Vasc Biol 28:1477-83. 2008
    ..However, the effects of 5'-NT treatment on platelet aggregation remain unknown. We examined the direct effects of 5'-NT treatment on platelet aggregation in vivo and ex vivo using a whole blood aggregation method...