Manfred Schmitt

Summary

Affiliation: Klinikum rechts der Isar
Country: Germany

Publications

  1. ncbi request reprint Clinical utility of urokinase-type plasminogen activator and plasminogen activator inhibitor-1 determination in primary breast cancer tissue for individualized therapy concepts
    Nadia Harbeck
    Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany
    Clin Breast Cancer 3:196-200. 2002
  2. doi request reprint Multicenter study using paraffin-embedded tumor tissue testing PITX2 DNA methylation as a marker for outcome prediction in tamoxifen-treated, node-negative breast cancer patients
    Nadia Harbeck
    Department of Obstetrics and Gynecology and Pathology, Technical University of Munich, Munich, Germany
    J Clin Oncol 26:5036-42. 2008
  3. pmc Modulation of CXCR3 ligand secretion by prostaglandin E2 and cyclooxygenase inhibitors in human breast cancer
    Holger Bronger
    Department of Gynecology and Obstetrics, Technische Universitat Munchen, Ismaninger Str, 22, 81675 Munich, Germany
    Breast Cancer Res 14:R30. 2012
  4. doi request reprint Clinical utility of kallikrein-related peptidases (KLK) in urogenital malignancies
    J Dorn
    Prof Dr Manfred Schmitt, Klinische Forschergruppe der Frauenklinik der Technischen Universitaet Muenchen, Ismaninger Strasse 22, 81675 Muenchen, Germany, Tel 49 89 4140 2449, Fax 49 89 4140 7410, E mail
    Thromb Haemost 110:408-22. 2013
  5. ncbi request reprint Identification, validation, and clinical implementation of tumor-associated biomarkers to improve therapy concepts, survival, and quality of life of cancer patients: tasks of the Receptor and Biomarker Group of the European Organization for Research and T
    Manfred Schmitt
    Clinical Research Unit, Department of Obstetrics and Gynecology, Klinikum rechts der Isar, Technische Universitaet Muenchen, Ismaninger Strasse 22, D 81675 Munich, Germany
    Int J Oncol 25:1397-406. 2004
  6. doi request reprint Clinical utility of level-of-evidence-1 disease forecast cancer biomarkers uPA and its inhibitor PAI-1
    Manfred Schmitt
    Frauenklinik der Technischen Universitaet Muenchen, Germany
    Expert Rev Mol Diagn 10:1051-67. 2010
  7. doi request reprint Cancer therapy trials employing level-of-evidence-1 disease forecast cancer biomarkers uPA and its inhibitor PAI-1
    Manfred Schmitt
    Clinical Research Unit, Department of Obstetrics and Gynecology Frauenklinik, Klinikum rechts der Isar of the Technical University of Munich, Ismaninger Strasse 22, D 81675 Munich, Germany
    Expert Rev Mol Diagn 11:617-34. 2011
  8. ncbi request reprint European Organisation for Research and Treatment of Cancer (EORTC) Pathobiology Group standard operating procedure for the preparation of human tumour tissue extracts suited for the quantitative analysis of tissue-associated biomarkers
    Manfred Schmitt
    Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Germany
    Eur J Cancer 43:835-44. 2007
  9. ncbi request reprint Inhibition of intraperitoneal tumor growth of human ovarian cancer cells by bi- and trifunctional inhibitors of tumor-associated proteolytic systems
    Janna Krol
    Klinische Forschergruppe der Frauenklinik, Technische Universitat Munchen, Klinikum rechts der Isar, D 81675 Munchen, Germany
    Biol Chem 384:1097-102. 2003
  10. doi request reprint Tumor suppressor KAI1 affects integrin alphavbeta3-mediated ovarian cancer cell adhesion, motility, and proliferation
    Zlatna Ruseva
    Department of Gynecology and Obstetrics, Clinical Research Unit, Technische Universitat Munchen, Munich, Germany
    Exp Cell Res 315:1759-71. 2009

Detail Information

Publications94

  1. ncbi request reprint Clinical utility of urokinase-type plasminogen activator and plasminogen activator inhibitor-1 determination in primary breast cancer tissue for individualized therapy concepts
    Nadia Harbeck
    Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany
    Clin Breast Cancer 3:196-200. 2002
    ..Thus, combined determination of the invasion factors uPA and PAI-1 supports risk-adapted individualized therapeutic strategies in patients with primary breast cancer, particularly in those with node-negative breast cancer...
  2. doi request reprint Multicenter study using paraffin-embedded tumor tissue testing PITX2 DNA methylation as a marker for outcome prediction in tamoxifen-treated, node-negative breast cancer patients
    Nadia Harbeck
    Department of Obstetrics and Gynecology and Pathology, Technical University of Munich, Munich, Germany
    J Clin Oncol 26:5036-42. 2008
    ....
  3. pmc Modulation of CXCR3 ligand secretion by prostaglandin E2 and cyclooxygenase inhibitors in human breast cancer
    Holger Bronger
    Department of Gynecology and Obstetrics, Technische Universitat Munchen, Ismaninger Str, 22, 81675 Munich, Germany
    Breast Cancer Res 14:R30. 2012
    ....
  4. doi request reprint Clinical utility of kallikrein-related peptidases (KLK) in urogenital malignancies
    J Dorn
    Prof Dr Manfred Schmitt, Klinische Forschergruppe der Frauenklinik der Technischen Universitaet Muenchen, Ismaninger Strasse 22, 81675 Muenchen, Germany, Tel 49 89 4140 2449, Fax 49 89 4140 7410, E mail
    Thromb Haemost 110:408-22. 2013
    ..Evidently, depending on the type of cancer or cancer stage, individual KLKs may show characteristics of a Janus-faced behaviour, by either expanding or inhibiting cancer progression and metastasis. ..
  5. ncbi request reprint Identification, validation, and clinical implementation of tumor-associated biomarkers to improve therapy concepts, survival, and quality of life of cancer patients: tasks of the Receptor and Biomarker Group of the European Organization for Research and T
    Manfred Schmitt
    Clinical Research Unit, Department of Obstetrics and Gynecology, Klinikum rechts der Isar, Technische Universitaet Muenchen, Ismaninger Strasse 22, D 81675 Munich, Germany
    Int J Oncol 25:1397-406. 2004
    ..With this approach, a major step forward in the knowledge and understanding of the biological role and the clinical utility of tumor-associated biomarkers would be anticipated...
  6. doi request reprint Clinical utility of level-of-evidence-1 disease forecast cancer biomarkers uPA and its inhibitor PAI-1
    Manfred Schmitt
    Frauenklinik der Technischen Universitaet Muenchen, Germany
    Expert Rev Mol Diagn 10:1051-67. 2010
    ..Furthermore, small synthetic molecules targeting uPA are currently in Phase II clinical trials in patients afflicted with advanced cancer of the ovary, breast or pancreas...
  7. doi request reprint Cancer therapy trials employing level-of-evidence-1 disease forecast cancer biomarkers uPA and its inhibitor PAI-1
    Manfred Schmitt
    Clinical Research Unit, Department of Obstetrics and Gynecology Frauenklinik, Klinikum rechts der Isar of the Technical University of Munich, Ismaninger Strasse 22, D 81675 Munich, Germany
    Expert Rev Mol Diagn 11:617-34. 2011
    ..The therapeutic effect of a uPA-derived small-size synthetic peptide (Å6) is tested in advanced ovarian cancer patients...
  8. ncbi request reprint European Organisation for Research and Treatment of Cancer (EORTC) Pathobiology Group standard operating procedure for the preparation of human tumour tissue extracts suited for the quantitative analysis of tissue-associated biomarkers
    Manfred Schmitt
    Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Germany
    Eur J Cancer 43:835-44. 2007
    ..The resulting tissue cell debris sediment is a rich source of genomic DNA...
  9. ncbi request reprint Inhibition of intraperitoneal tumor growth of human ovarian cancer cells by bi- and trifunctional inhibitors of tumor-associated proteolytic systems
    Janna Krol
    Klinische Forschergruppe der Frauenklinik, Technische Universitat Munchen, Klinikum rechts der Isar, D 81675 Munchen, Germany
    Biol Chem 384:1097-102. 2003
    ..These compact and small inhibitors may represent promising agents for gene therapy of solid malignant tumors...
  10. doi request reprint Tumor suppressor KAI1 affects integrin alphavbeta3-mediated ovarian cancer cell adhesion, motility, and proliferation
    Zlatna Ruseva
    Department of Gynecology and Obstetrics, Clinical Research Unit, Technische Universitat Munchen, Munich, Germany
    Exp Cell Res 315:1759-71. 2009
    ..These data strongly indicate that KAI1 may suppress ovarian cancer progression by inhibiting integrin alphavbeta3/vitronectin-provoked tumor cell motility and proliferation as important hallmarks of the oncogenic process...
  11. ncbi request reprint Disease processes may be reflected by correlations among tissue kallikrein proteases but not with proteolytic factors uPA and PAI-1 in primary ovarian carcinoma
    Julia Dorn
    Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, D 81675 Munich, Germany, and Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Canada
    Biol Chem 387:1121-8. 2006
    ..These results support the notion that several tissue kallikreins are co-expressed in ovarian cancer patients, substantiating the existence of a steroid hormone-driven tissue kallikrein cascade in this disease...
  12. ncbi request reprint Overexpression of the human tissue kallikrein genes KLK4, 5, 6, and 7 increases the malignant phenotype of ovarian cancer cells
    Panagiotis Prezas
    Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, D 81675 Munich, Germany, and G Papanicolaou Research Center of Oncology, Saint Savas Hospital, GR 11522 Athens, Greece
    Biol Chem 387:807-11. 2006
    ..017; chi2 test). Our results strongly support the view that tumor-associated overexpression of tissue kallikreins contributes to ovarian cancer progression...
  13. ncbi request reprint ADAM15 decreases integrin alphavbeta3/vitronectin-mediated ovarian cancer cell adhesion and motility in an RGD-dependent fashion
    Veronika Beck
    Clinical Research Unit, Department of Obstetrics and Gynecology, Technische Universität München TUM, D 81675 Munich, Germany
    Int J Biochem Cell Biol 37:590-603. 2005
    ..In conclusion, our results point to a physiological role of ADAM15 as a natural binding partner of integrin alphavbeta3 thereby loosening tumor cell adhesion to the underlying matrix and regulating tumor cell migration and invasion...
  14. ncbi request reprint Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes
    Bettina Grismayer
    Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Ismaninger Str 22, D 81675 Munich, Germany
    Biol Chem 393:1449-55. 2012
    ..uPAR-del4/5 may thus play an important role in the regulation of the extracellular proteolytic network and, by this, influence the metastatic potential of breast cancer cells...
  15. ncbi request reprint Quantitative RT-PCR assays for the determination of urokinase-type plasminogen activator and plasminogen activator inhibitor type 1 mRNA in primary tumor tissue of breast cancer patients: comparison to antigen quantification by ELISA
    Julia C Biermann
    Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, D 81675 Munich, Germany
    Int J Mol Med 21:251-9. 2008
    ..In conclusion, the implementation of mRNA quantification of uPA and PAI-1 in breast tumors is unable to serve as a one-to-one substitution for antigen determination by ELISA...
  16. pmc Immunohistochemical evidence of ubiquitous distribution of the metalloendoprotease insulin-degrading enzyme (IDE; insulysin) in human non-malignant tissues and tumor cell lines
    Gregor Weirich
    Institute of Pathology, Klinikum rechts der Isar, Technical University of Munich, Ismaninger Str 22, D 81675 Munich, Germany
    Biol Chem 389:1441-5. 2008
    ..IDE protein was expressed in all the tissues assessed and all the tumor cell lines except for Raji and HL-60. Trophoblast cells and granulocytes, but not normal lymphocytes, were also IDE-positive...
  17. doi request reprint Overexpression of the urokinase receptor mRNA splice variant uPAR-del4/5 affects tumor-associated processes of breast cancer cells in vitro and in vivo
    Sumito Sato
    Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University Munich, Ismaninger Str 22, 81675 Munich, Germany
    Breast Cancer Res Treat 127:649-57. 2011
    ..Our results thus illustrate how tumor-specific alternative splicing can distinctly impact the biology of the tumor...
  18. pmc Expression of metalloprotease insulin-degrading enzyme insulysin in normal and malignant human tissues
    Christina Yfanti
    Department of Obstetrics and Gynecology, Klinikum rechts der Isar, Technical University of Munich, D 81675 Munich, Germany
    Int J Mol Med 22:421-31. 2008
    ....
  19. pmc Rab31 expression levels modulate tumor-relevant characteristics of breast cancer cells
    Bettina Grismayer
    Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Ismaninger Str, 22, Munich 81675, Germany
    Mol Cancer 11:62. 2012
    ..In the present study, we aimed to investigate the impact of rab31 (over)-expression on important aspects of tumor progression in vitro and in vivo...
  20. ncbi request reprint Primary tumor levels of human tissue kallikreins affect surgical success and survival in ovarian cancer patients
    Julia Dorn
    Department of Obstetrics and Gynecology, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany
    Clin Cancer Res 13:1742-8. 2007
    ....
  21. ncbi request reprint Urokinase-type plasminogen activator and its inhibitor type 1 predict disease outcome and therapy response in primary breast cancer
    Nadia Harbeck
    Department of Obstetrics and Gynecology, Technical University of Munich, Germany
    Clin Breast Cancer 5:348-52. 2004
    ....
  22. doi request reprint Characteristics of the level-of-evidence-1 disease forecast cancer biomarkers uPA and its inhibitor PAI-1
    Karin Mengele
    Klinikum rechts der Isar of the Technische Universität München, Ismaninger Strasse 22, D 81675 Munich, Germany
    Expert Rev Mol Diagn 10:947-62. 2010
    ....
  23. ncbi request reprint Enhanced benefit from adjuvant chemotherapy in breast cancer patients classified high-risk according to urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor type 1 (n = 3424)
    Nadia Harbeck
    Department of Obstetrics and Gynecology, Technical University, D 81675 Munich, Germany
    Cancer Res 62:4617-22. 2002
    ....
  24. ncbi request reprint Inhibition of the tumor-associated urokinase-type plasminogen activation system: effects of high-level synthesis of soluble urokinase receptor in ovarian and breast cancer cells in vitro and in vivo
    Viktor Magdolen
    Klinische Forschergruppe der Frauenklinik der TU München, Klinikum rechts der Isar, Ismaninger Str 22, 81675 Munich, Germany
    Recent Results Cancer Res 162:43-63. 2003
    ....
  25. ncbi request reprint Stromal cell-associated expression of kallikrein-related peptidase 6 (KLK6) indicates poor prognosis of ovarian cancer patients
    Lina Seiz
    Klinische Forschergruppe der Frauenklinik der Technischen Universität München, Klinikum rechts der Isar, Munich, Germany
    Biol Chem 393:391-401. 2012
    ..low KLK6 expression; hazard ratio (HR), 1.92; p=0.017; HR, 1.80; p=0.042, respectively]. These results indicate that KLK6 expressed by stromal cells may considerably contribute to the aggressiveness of ovarian cancer...
  26. ncbi request reprint Urokinase-type plasminogen activator (uPA) and its inhibitor PAI-I: novel tumor-derived factors with a high prognostic and predictive impact in breast cancer
    Nadia Harbeck
    Department of Obstetrics and Gynecology, Technical University of Munich, Germany
    Thromb Haemost 91:450-6. 2004
    ....
  27. ncbi request reprint High hydrostatic pressure-induced cell death in human chondrocytes and chondrosarcoma cells
    Florian Dominique Naal
    Department of Orthopedic Surgery, Technical University of Munich, Ismaninger Str 22, D 81675 Muenchen, Germany
    Anticancer Res 25:1977-82. 2005
    ....
  28. ncbi request reprint Revitalization of human bone after extracorporeal high hydrostatic pressure treatment
    Johannes Schauwecker
    Department of Orthopedic Surgery, Technical University of Munich, Germany
    Anticancer Res 31:1235-9. 2011
    ..The subject of the present study is revitalization of human bone segments after HHP treatment to acquire knowledge about the ingrowth and regeneration of osteoblast-like cells after such treatment...
  29. ncbi request reprint Methods to analyze the effects of the urokinase system on cancer cell adhesion, proliferation, migration, and signal transduction events
    Ute Reuning
    Department of Obstetrics and Gynaecology, Technical University of Munich, Germany
    Methods Mol Med 120:427-40. 2006
    ..g., as a function of uPA-R/integrin-mediated effects...
  30. ncbi request reprint Effect of high hydrostatic pressure on biological properties of extracellular bone matrix proteins
    Peter Diehl
    Department of Orthopedic Surgery, Technical University of Munich, Munich, Germany
    Int J Mol Med 16:285-9. 2005
    ..While during this treatment eukaryotic cells including tumor cells will be irreversibly impaired, the bone's biomechanical properties and the biological properties of the ECM proteins FN, VN, and Col-I, respectively, are preserved...
  31. ncbi request reprint Effect of extracorporeal high hydrostatic pressure on cellular outgrowth from tumor-afflicted bone
    Johannes Schauwecker
    Department of Orthopedic Surgery, Technical University of Munich, Germany
    Anticancer Res 26:85-9. 2006
    ....
  32. ncbi request reprint Urokinase-type plasminogen activator (uPA) and its receptor (uPAR): development of antagonists of uPA/uPAR interaction and their effects in vitro and in vivo
    Ute Reuning
    Klinische Forschergruppe der Frauenklinik, Technische Universitat Munchen, Ismaninger Str 22, D 81675 Munich, Germany
    Curr Pharm Des 9:1529-43. 2003
    ..Such compounds affecting the uPA/uPAR system represent novel tumor biology-based therapeutic agents, thereby opening new ways for patient optimized and individualized cancer therapy...
  33. ncbi request reprint Molecular and functional interdependence of the urokinase-type plasminogen activator system with integrins
    Ute Reuning
    Klinische Forschergruppe, Frauenklinik der Technischen Universitat Munchen, Klinikum rechts der Isar, D 81675 Munchen, Germany
    Biol Chem 384:1119-31. 2003
    ..By this multifaceted crosstalk, cells may modulate their proteolytic, adhesive, and migratory activities and monitor ECM integrity in their microenvironment...
  34. ncbi request reprint Novel bi- and trifunctional inhibitors of tumor-associated proteolytic systems
    Janna Krol
    Klinische Forschergruppe der Frauenklinik der Technischen Universität München, Klinikum rechts der Isar, D 81675 Munchen, Germany
    Biol Chem 384:1085-96. 2003
    ..Thus, these novel, compact, and small-size inhibitors directed against up to three different tumor-associated proteolytic systems may represent promising agents for prevention of tumor cell migration and metastasis...
  35. ncbi request reprint Procedures for the quantitative protein determination of urokinase and its inhibitor, PAI-1, in human breast cancer tissue extracts by ELISA
    Manfred Schmitt
    Clinical Research Unit, Department of Obstetrics and Gynaecology, Technical University of Munich, Germany
    Methods Mol Med 120:245-65. 2006
    ..Such a technique allows rapid and quantitative determination of uPA and PAI-1, even in small breast cancer specimens...
  36. ncbi request reprint Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa
    Nathalie Beaufort
    Department of Obstetrics and Gynecology, Technical University of Munich, Ismaninger Strasse 22, D 81675 Munich, Germany
    Biochem J 428:473-82. 2010
    ..Direct pro-uPA activation, as also reported for other bacterial proteases, might be a frequent phenomenon that contributes to bacterial virulence...
  37. ncbi request reprint Tumor-associated proteolytic factors uPA and PAI-1: critical appraisal of their clinical relevance in breast cancer and their integration into decision-support algorithms
    Nadia Harbeck
    Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany
    Crit Rev Clin Lab Sci 44:179-201. 2007
    ..This concept could be useful in assessing and maximizing the performance of risk assessment and the quality of therapeutic indications...
  38. ncbi request reprint Analysis of the DPYD gene implicated in 5-fluorouracil catabolism in a cohort of Caucasian individuals
    Katharina Seck
    Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany
    Clin Cancer Res 11:5886-92. 2005
    ..The intention of this population study is to assess and to evaluate gene variations in the entire coding region of the dihydropyrimidine dehydrogenase gene (DPYD), which could be implicated in DPD malfunction...
  39. ncbi request reprint Effect of extracorporal high hydrostatic pressure on tumor cell adherence and viability
    Peter Diehl
    Department of Orthopedic Surgery, Technical University of Munich, Germany
    Oncol Rep 12:369-73. 2004
    ..Therefore, we anticipate that in orthopedic surgery HHP could be used as a new gentle way of treating resected cancer-afflicted bones or tendons to inactivate tumor cells before autologous reimplantation...
  40. doi request reprint Polyclonal antibodies against kallikrein-related peptidase 4 (KLK4): immunohistochemical assessment of KLK4 expression in healthy tissues and prostate cancer
    Lina Seiz
    Department of Obstetrics and Gynecology, Technical University of Munich, Germany
    Biol Chem 391:391-401. 2010
    ..Thus, our results indicate that KLK4, which is expressed in the healthy prostate, is upregulated in early-stage but not late-stage prostate cancer...
  41. ncbi request reprint Protein expression of MMP-13, uPA, and PAI-1 in pseudocapsular and interface tissue around implants of loose artificial hip joints and in osteoarthritis
    Peter Diehl
    Department of Orthopaedic Surgery, Technical University of Munich, D 81675 Munchen, Germany
    Int J Mol Med 13:711-5. 2004
    ....
  42. doi request reprint Interdependence of kallikrein-related peptidases in proteolytic networks
    Nathalie Beaufort
    Department of Obstetrics and Gynecology, Technical University of Munich, Germany
    Biol Chem 391:581-7. 2010
    ..Our observations provide new insights into the regulation of these important tumor-associated proteases...
  43. ncbi request reprint Spontaneous in vitro transformation of primary human osteoblast-like cells
    Ursula Magdolen
    Department of Orthopedic Surgery, Klinikum rechts der Isar, Technische Universitaet Muenchen, D 81675 Muenchen, Germany
    Cancer Genomics Proteomics 7:61-6. 2010
    ..Two new tumor-like cell lines were established which developed spontaneously in vitro from normal human primary osteoblast-like cells originating from non-oncogenic bone surgery...
  44. ncbi request reprint Non-muscle alpha-actinin-4 interacts with plasminogen activator inhibitor type-1 (PAI-1)
    Ulla Magdolen
    Klinische Forschergruppe der Frauenklinik der Technischen Universität München, Klinikum rechts der Isar, Ismaninger Str 22, D 81675 Munchen, Germany
    Biol Chem 385:801-8. 2004
    ..We suggest that PAI-1, via interaction with both Act-4 and uPA, may function as a modulator of this mononuclear phagocyte response, not only in inflammation but also in tumor invasion and metastasis...
  45. ncbi request reprint Biomechanical and immunohistochemical analysis of high hydrostatic pressure-treated Achilles tendons
    Peter Diehl
    Department of Orthopedic Surgery, Technical University of Munich, Ismaninger Str 22, D 81675 Munich, Germany
    J Orthop Sci 11:380-5. 2006
    ..The aim of this study was to examine the biomechanical and immunohistochemical properties of tendons after exposure to HHP and to evaluate whether preservation of the bony attachment of tendons and ligaments is possible...
  46. doi request reprint Integrin alphavbeta3 upregulates integrin-linked kinase expression in human ovarian cancer cells via enhancement of ILK gene transcription
    Daniela Lössner
    Clinical Research Unit, Frauenklinik der Technischen Universitat Munchen, Munich, Germany
    J Cell Physiol 220:367-75. 2009
    ..By increasing ILK as an important integrin-proximal kinase, alphavbeta3 may promote its intracellular signaling and tumor biological processes arising thereof in favor of ovarian cancer metastasis...
  47. doi request reprint Function and clinical relevance of kallikrein-related peptidases and other serine proteases in gynecological cancers
    Julia Dorn
    Klinische Forschergruppe der Frauenklinik der Technischen Universität München, Klinikum rechts der Isar, Munich, Germany
    Crit Rev Clin Lab Sci 51:63-84. 2014
    ..In conclusion, proteases are a rich source of biomarkers of gynecological cancer, though the enzymes' exact roles and functions merit further investigation...
  48. ncbi request reprint Minimal residual disease in breast cancer and gynecological malignancies: phenotype and clinical relevance
    Frigga Roggel
    Klinische Forschergruppe der Frauenklinik, Technische Universitat Munchen, Ismaninger Strasse 22, 81675 Munich, Germany
    Recent Results Cancer Res 162:89-100. 2003
    ....
  49. ncbi request reprint Tumour-associated urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 in normal and neoplastic tissues of patients with squamous cell cancer of the oral cavity - clinical relevance and prognostic value
    Brigitte Hundsdorfer
    Department of Oral and Cranio Maxillofacial Surgery, Technical University of Munich, Klinikum rechts der Isar, Munchen, Germany
    J Craniomaxillofac Surg 33:191-6. 2005
    ..These observations might help to select patients with poor prognosis for additional adjuvant therapy in conjunction with complete surgical resection...
  50. ncbi request reprint The human fibrinolytic system is a target for the staphylococcal metalloprotease aureolysin
    Nathalie Beaufort
    Department of Obstetrics and Gynecology, Technical University of Munich, Ismaninger Str 22, D 81675 Munich, Germany
    Biochem J 410:157-65. 2008
    ..aureus, and thus may promote bacterial spread and invasion...
  51. doi request reprint Emerging biomarkers in breast cancer care
    Rudolf Napieralski
    Clinical Research Unit, Department of Obstetrics and Gynecology, Klinikum rechts der Isar, Technische Universitaet Muenchen, Munich, Germany
    Biomark Med 4:505-22. 2010
    ..These biomarkers are now subject of validation in prospective clinical trials...
  52. ncbi request reprint High-affinity urokinase-derived cyclic peptides inhibiting urokinase/urokinase receptor-interaction: effects on tumor growth and spread
    Sumito Sato
    Klinische Forschergruppe der Frauenklinik, Klinikum rechts der Isar, Technische Universitat Munchen, Ismaninger Str 22, D 81675, Munchen, Germany
    FEBS Lett 528:212-6. 2002
    ..Thus, both WX-360 and WX-360-Nle are promising novel compounds to reduce dissemination of human ovarian carcinoma...
  53. ncbi request reprint Quantitative reverse transcription-PCR assay for detection of mRNA encoding full-length human tissue kallikrein 7: prognostic relevance of KLK7 mRNA expression in breast cancer
    Leon Holzscheiter
    Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany
    Clin Chem 52:1070-9. 2006
    ..The human tissue kallikrein gene family (KLK1 to KLK15) encodes a group of 15 serine proteases (hK1 to hK15), several of which have been implicated in cancer-related processes...
  54. ncbi request reprint Immunohistochemical demonstration of the zinc metalloprotease insulin-degrading enzyme in normal and malignant human breast: correlation with tissue insulin levels
    Razvan T Radulescu
    Clinical Research Unit, Department of Obstetrics and Gynecology, Klinikum rechts der Isar, Technical University of Munich, Ismaninger Str 22, D 81675 Munchen, Germany
    Int J Oncol 30:73-80. 2007
    ..Our present report should also provide an experimental starting point towards exploring a potential role of IDE in the control of tumor progression...
  55. ncbi request reprint Ex vivo detection of apoptotic trophoblast cells applying flow cytofluorometry and immunocytochemistry using M30 antibody directed to the cytokeratin 18 neo-epitope
    Janna Krol
    Klinische Forschergruppe der Frauenklinik der Technischen Universitaet Muenchen, Klinikum rechts der Isar, Germany
    Int J Mol Med 16:415-20. 2005
    ..Our protocol is an improvement over previously used methods such as immunocytochemistry as it allows to differentiate rapidly between competent and apoptotic trophoblast cells by the quantitative method of flow cytofluorometry...
  56. ncbi request reprint Content of urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 in oral mucosa and inflamed periodontal tissue
    Herbert Deppe
    Department of Oral and Craniomaxillofacial Surgery, Technische Universitat Munchen, Klinikum rechts der Isar, Munich, Germany
    Quintessence Int 41:165-71. 2010
    ....
  57. doi request reprint Placental expression of proteases and their inhibitors in patients with HELLP syndrome
    Stephanie Pildner von Steinburg
    Abteilung Perinatalmedizin, Frauenklinik und Poliklinik der Technischen Universität München, Klinikum rechts der Isar, D 81675 Munchen, Germany
    Biol Chem 390:1199-204. 2009
    ..Our findings at the mRNA level suggest a decrease in matrix remodeling in placentae from patients with HELLP syndrome compared with control pregnancies, although this is not supported at the protein level...
  58. ncbi request reprint Ovarian cancer cell proliferation and motility is induced by engagement of integrin alpha(v)beta3/Vitronectin interaction
    Sandra Hapke
    Department of Obstetrics and Gynecology, Clinical Research Unit, Technical University Munich, D 81675 Munich, Germany
    Biol Chem 384:1073-83. 2003
    ....
  59. ncbi request reprint Induction of tumor cell death by high hydrostatic pressure as a novel supporting technique in orthopedic surgery
    Peter Diehl
    Orthopaedische Klinik und Poliklinik der Technischen Universitaet Muenchen, D 81675 Muenchen, Germany
    Oncol Rep 10:1851-5. 2003
    ..The present study demonstrates that both normal cells and tumor cells are rapidly inactivated by HHP treatment...
  60. ncbi request reprint Quantitative analysis of the impact of short-time high hydrostatic pressure on bone tumor-associated proteases
    Peter Diehl
    Department of Orthopedic Surgery, University of Rostock, Rostock, Germany
    Int J Mol Med 19:667-73. 2007
    ..During this treatment tumor cells are irreversibly impaired, while HHP treatment of proteases may not exert any significant autolytic effect on bone tissue...
  61. ncbi request reprint Growth promoting in vitro effect of synthetic cyclic RGD-peptides on human osteoblast-like cells attached to cancellous bone
    Ursula Magdolen
    Department of Orthopedic Surgery, Muenchen, Germany
    Int J Mol Med 17:1017-21. 2006
    ....
  62. ncbi request reprint Clinical relevance of invasion factors urokinase-type plasminogen activator and plasminogen activator inhibitor type 1 for individualized therapy decisions in primary breast cancer is greatest when used in combination
    Nadia Harbeck
    Clinical Research Group, Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany
    J Clin Oncol 20:1000-7. 2002
    ..The improvement in clinical risk assessment gained by combining these factors is evaluated here...
  63. ncbi request reprint Detailed analysis of five mutations in dihydropyrimidine dehydrogenase detected in cancer patients with 5-fluorouracil-related side effects
    Eva Gross
    Department of Obstetrics and Gynecology, Technical University of Munich, Germany
    Hum Mutat 22:498. 2003
    ....
  64. ncbi request reprint Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin (Vn): mapping the binding sites on PAI-1 and Vn
    Florian Schroeck
    Klinische Forschergruppe der Frauenklinik der Technischen Universität München, Klinikum rechts der Isar, Germany
    Biol Chem 383:1143-9. 2002
    ..There are indications for at least one other low-affinity PAI-1 binding site in the C-terminal region of Vn, which seems to be involved in the formation of larger PAI-1/Vn complexes...
  65. ncbi request reprint HELLP syndrome with fetal growth retardation in a woman homozygous for the prothrombin gene variant 20210A
    Georg Moessmer
    Institute of Clinical Chemistry, Technische Universitat Munchen, Klinikum rechts der Isar, Ismaninger Strasse 22, 81675 Munchen, Germany
    Thromb Haemost 93:787-8. 2005
  66. ncbi request reprint Advanced statistical methods for the definition of new staging models
    Ronald Kates
    Klinische Forschergruppe der Frauenklinik, Technische Universitat Munchen, Ismaningerstr 22, 81675 Munich, Germany
    Recent Results Cancer Res 162:101-13. 2003
    ..In this article, advantages of advanced statistical methods such as neural networks for definition of new staging models will be illustrated using breast cancer as an example...
  67. ncbi request reprint Invasion factors uPA/PAI-1 and HER2 status provide independent and complementary information on patient outcome in node-negative breast cancer
    Iris Zemzoum
    Frauenklinik and Institut für Allgemeine Pathologie und Pathologische Anatomie, Technische Universitat, and Gemeinschaftspraxis Lachnerstrasse 2 für Pathologie und Zytologie, Munchen, Germany
    J Clin Oncol 21:1022-8. 2003
    ....
  68. ncbi request reprint Human kallikrein 8 protein is a favorable prognostic marker in ovarian cancer
    Carla A Borgono
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, University of Toronto, Ontario, Canada
    Clin Cancer Res 12:1487-93. 2006
    ..001 and P = 0.014, respectively). These results indicate that hK8 is an independent marker of favorable prognosis in ovarian cancer...
  69. ncbi request reprint DNA-methylation of the homeodomain transcription factor PITX2 reliably predicts risk of distant disease recurrence in tamoxifen-treated, node-negative breast cancer patients--Technical and clinical validation in a multi-centre setting in collaboration wit
    Sabine Maier
    Department of Research, Epigenomics AG, Berlin, Germany
    Eur J Cancer 43:1679-86. 2007
    ..In addition, these low-risk postmenopausal patients seem to respond sufficiently well to tamoxifen so that they may not require up-front aromatase inhibitor therapy...
  70. ncbi request reprint Multicenter validation of a gene expression-based prognostic signature in lymph node-negative primary breast cancer
    John A Foekens
    Department of Medical Oncology, Erasmus Medical Center, Daniel den Hoed Cancer Center, Rotterdam, The Netherlands
    J Clin Oncol 24:1665-71. 2006
    ..The aim of this study was to validate this gene signature in an independent more diverse population of LNN patients from multiple institutions...
  71. ncbi request reprint Cancer progression and tumor cell motility are associated with the FGFR4 Arg(388) allele
    Johannes Bange
    Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Cancer Res 62:840-7. 2002
    ..Our results support the conclusion that the FGFR4 Arg(388) allele represents a determinant that is innocuous in healthy individuals but predisposes cancer patients for significantly accelerated disease progression...
  72. ncbi request reprint Pooled analysis of prognostic impact of urokinase-type plasminogen activator and its inhibitor PAI-1 in 8377 breast cancer patients
    Maxime P Look
    Department of Medical Oncology, University Hospital Rotterdam, The Netherlands
    J Natl Cancer Inst 94:116-28. 2002
    ....
  73. ncbi request reprint Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin
    Nuria Arroyo De Prada
    Klinische Forschergruppe der Frauenklinik der Technischen Universität München, Klinikum rechts der Isar, German
    Eur J Biochem 269:184-92. 2002
    ..Our results demonstrate that the wild-type sequence of the region around hE in PAI-1 is not a prerequisite for binding to Vn...
  74. ncbi request reprint Y-box factor YB-1 predicts drug resistance and patient outcome in breast cancer independent of clinically relevant tumor biologic factors HER2, uPA and PAI-1
    Martin Janz
    Department of Cell Growth and Differentiation, Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Int J Cancer 97:278-82. 2002
    ....
  75. ncbi request reprint Identification of a novel urokinase receptor splice variant and its prognostic relevance in breast cancer
    Thomas Luther
    Institut für Pathlogie, Technische Universitat Dresden, Germany
    Thromb Haemost 89:705-17. 2003
    ..Higher uPAR-del4/5 expression was significantly associated with shorter disease-free survival (p = 0.0004) of breast cancer patients. These results suggest that uPAR-del4/5 mRNA may serve as a novel prognostic marker in breast cancer...
  76. ncbi request reprint Association of DNA methylation of phosphoserine aminotransferase with response to endocrine therapy in patients with recurrent breast cancer
    John W M Martens
    Department of Medical Oncology, Erasmus MC, Rotterdam, The Netherlands
    Cancer Res 65:4101-17. 2005
    ..Concluding, our work indicates that promoter hypermethylation and mRNA expression of PSAT1 are indicators of response to tamoxifen-based endocrine therapy in steroid hormone receptor-positive patients with recurrent breast cancer...
  77. ncbi request reprint Considerations on development, validation, application, and quality control of immuno(metric) biomarker assays in clinical cancer research: an EORTC-NCI working group report
    Fred C G J Sweep
    Department of Chemical Endocrinology, University Medical Center Nijmegen, NL 6500 HB Nijmegen, The Netherlands
    Int J Oncol 23:1715-26. 2003
    ..Moreover, a model for an appropriate quality assurance program is presented...
  78. ncbi request reprint Effect of reoxygenation on the hypoxia-induced up-regulation of serine protease inhibitor PAI-1 in head and neck cancer cells
    Lisa D Sprague
    Institute of Molecular Pathogenesis, Friedrich Loeffler Institut, Jena, Germany
    Oncology 71:282-91. 2006
    ..Fatally irradiated reoxygenat- ed tumour cells might still produce sufficient amounts of 'harmful' protein and thus initiate a path for invasion and metastasis for surviving tumour cells...
  79. ncbi request reprint Urokinase receptor splice variant uPAR-del4/5-associated gene expression in breast cancer: identification of rab31 as an independent prognostic factor
    Matthias Kotzsch
    Institute of Pathology, Dresden University of Technology, Fetscherstr 74, 01307, Dresden, Germany
    Breast Cancer Res Treat 111:229-40. 2008
    ..To evaluate the pure prognostic impact of the uPA-receptor splice variant uPAR-del4/5 for lymph node-negative breast cancer patients, and to identify differentially expressed genes associated with high or low uPAR-del4/5 mRNA levels...
  80. ncbi request reprint DNA hypermethylation of PITX2 is a marker of poor prognosis in untreated lymph node-negative hormone receptor-positive breast cancer patients
    Inko Nimmrich
    Department of Biomedical Research and Development and Technology Development, Epigenomics AG, Berlin, Germany
    Breast Cancer Res Treat 111:429-37. 2008
    ..In addition, we studied the association between PITX2 DNA methylation and PITX2 gene expression...
  81. ncbi request reprint Effects of hypoxia and reoxygenation on the expression levels of the urokinase-type plasminogen activator, its inhibitor plasminogen activator inhibitor type-1 and the urokinase-type plasminogen activator receptor in human head and neck tumour cells
    Lisa D Sprague
    Institut für Molekulare Pathogenese, Friedrich Loeffler Institut, Bundesforschungsinstitut für Tiergesundheit, D 07743 Jena, Germany
    Oncol Rep 17:1259-68. 2007
    ....
  82. ncbi request reprint Urokinase-type plasminogen activator system in breast cancer: association with tamoxifen therapy in recurrent disease
    Marion E Meijer-Van Gelder
    Department of Medical Oncology, Erasmus MC Rotterdam, Rotterdam, The Netherlands
    Cancer Res 64:4563-8. 2004
    ..Knowledge of their tumor expression levels might be helpful for future individualized therapy protocols, including possible new-targeted therapies based on the interference in the urokinase system...
  83. doi request reprint Intrathecal trastuzumab (Herceptin) and methotrexate for meningeal carcinomatosis in HER2-overexpressing metastatic breast cancer: a case report
    Hans Joachim Stemmler
    Medical Department III, Ludwig Maximilians University of Munich, Klinikum Grosshadern, Munich, Germany
    Anticancer Drugs 19:832-6. 2008
    ..This case report gives details regarding the clinical course of a breast cancer patient who received intrathecal trastuzumab and methotrexate via lumbar puncture for meningeal carcinomatosis of HER2-overexpressing MBC...
  84. ncbi request reprint Suppression of rat breast cancer metastasis and reduction of primary tumour growth by the small synthetic urokinase inhibitor WX-UK1
    Buddy Setyono-Han
    Department of Medical Oncology, Erasmus Medical Center Rotterdam, 3000 DR Rotterdam, The Netherlands
    Thromb Haemost 93:779-86. 2005
    ..In conclusion, our results provide evidence that WX-UK1 as a single agent inhibits breast tumour growth and metastasis in vivo, and thus is a promising candidate drug to treat human cancer...
  85. ncbi request reprint Clinical relevance of matrix metalloproteinase-13 determined with a new highly specific and sensitive ELISA in ascitic fluid of advanced ovarian carcinoma patients
    Bernd Hantke
    Department of Chemistry, Biochemistry I, University of Bielefeld, D 33615 Bielefeld, Germany
    Biol Chem 384:1247-51. 2003
    ..Together with other prognostic markers, determination of MMP-13 in ascitic fluid may help to identify patients at risk for early death and help to individualize adjuvant therapy...
  86. ncbi request reprint Prognostic relevance of uPAR-del4/5 and TIMP-3 mRNA expression levels in breast cancer
    Matthias Kotzsch
    Institute of Pathology, Technical University Dresden, Fetscherstr 74, D 01307 Dresden, Germany
    Eur J Cancer 41:2760-8. 2005
    ..Therefore, considering the prognostic impact of two proteolytic factors stemming from complementary protease systems may improve the prediction of disease recurrence in breast cancer...
  87. ncbi request reprint Pooled analysis of prognostic impact of uPA and PAI-1 in breast cancer patients
    Maxime Look
    Erasmus MC, Josephine Nefkens Institute, Rm Be 428, P O Box 1738, 3000 DR Rotterdam, The Netherlands
    Thromb Haemost 90:538-48. 2003
    ..We conclude that uPA and PAI-1 are ready to be used in the clinic to help classify breast cancer patients into high and low risk groups...
  88. ncbi request reprint Synthesis, solution structure, and biological evaluation of urokinase type plasminogen activator (uPA)-derived receptor binding domain mimetics
    Niko Schmiedeberg
    Institut fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, D 85747 Garching, Germany
    J Med Chem 45:4984-94. 2002
    ..In conclusion, small cyclic peptides were created, which mimic the structure and activity of the binding epitope of uPA to uPAR and which may serve as novel therapeutic agents in cancer metastasis...
  89. ncbi request reprint Biomolecular features of clinical relevance in breast cancer
    Maria Grazia Daidone
    Research Unit 10, Department of Experimental Oncology, Istituto Nazionale Tumori, Via Venezian 1, 20133 Milan, Italy
    Eur J Nucl Med Mol Imaging 31:S3-14. 2004
    ..However, the outcome of these novel studies still needs to be validated, which will entail cooperation between different specialists and integration of all the different skills involved in translational research in oncology...
  90. ncbi request reprint uPA-silica-Particles (SP-uPA): a novel analytical system to investigate uPA-uPAR interaction and to test synthetic uPAR antagonists as potential cancer therapeutics
    Elke Guthaus
    Klinische Forschergruppe, Frauenklinik der TU München, Germany
    Biol Chem 383:207-16. 2002
    ..The results obtained with the noncellular SP-uPA/uPAR system are highly comparable to those obtained with a cellular system involving FITC-uPA and the promyeloid cell line U937 as the source of uPAR...
  91. ncbi request reprint Tumor markers: from laboratory to clinical utility
    Anne Sofie Schrohl
    Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Ridebanevej 9, DK 1870 Frederiksberg C, Copenhagen, Denmark
    Mol Cell Proteomics 2:378-87. 2003
  92. ncbi request reprint Human kallikrein 5: a potential novel serum biomarker for breast and ovarian cancer
    George M Yousef
    Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5 Canada
    Cancer Res 63:3958-65. 2003
    ..Our preliminary data indicate that hK5 is a potential biomarker in patients with ovarian and breast cancer...