F Hofmann

Summary

Affiliation: Klinikum rechts der Isar
Country: Germany

Publications

  1. ncbi request reprint International Union of Pharmacology. XLII. Compendium of voltage-gated ion channels: cyclic nucleotide-modulated channels
    Franz Hofmann
    Institut fur Pharmakologie und Toxikologie, Technischen Universitat Munchen, Biedersteiner Strasse 29, D 80802 Munich, Germany
    Pharmacol Rev 55:587-9. 2003
  2. ncbi request reprint Function of cGMP-dependent protein kinases as revealed by gene deletion
    F Hofmann
    Institut für Pharmakologie und Toxicologie, Technische Universitat Munchen, Biedersteiner Strasse 29, D 80802 Munich, Germany
    Physiol Rev 86:1-23. 2006
  3. ncbi request reprint International Union of Pharmacology. LI. Nomenclature and structure-function relationships of cyclic nucleotide-regulated channels
    Franz Hofmann
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner StraBe 29, D 80802 München, Germany
    Pharmacol Rev 57:455-62. 2005
  4. ncbi request reprint The role of subunit composition on prepulse facilitation of the cardiac L-type calcium channel
    S Dai
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Munich, Germany
    FEBS Lett 442:70-4. 1999
  5. ncbi request reprint Distribution of IRAG and cGKI-isoforms in murine tissues
    A Geiselhöringer
    Institut für Pharmakologie und Toxikologie der TU München, Biedersteiner Strasse 29, 80802 Munchen, Germany
    FEBS Lett 575:19-22. 2004
  6. pmc Two pacemaker channels from human heart with profoundly different activation kinetics
    A Ludwig
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    EMBO J 18:2323-9. 1999
  7. pmc IRAG is essential for relaxation of receptor-triggered smooth muscle contraction by cGMP kinase
    Angela Geiselhöringer
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    EMBO J 23:4222-31. 2004
  8. ncbi request reprint Cellular expression and functional characterization of four hyperpolarization-activated pacemaker channels in cardiac and neuronal tissues
    S Moosmang
    , , Germany
    Eur J Biochem 268:1646-52. 2001
  9. doi request reprint Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle
    Eva Frei
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Munich, Germany
    Am J Physiol Gastrointest Liver Physiol 297:G834-9. 2009
  10. ncbi request reprint Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4-/- mice
    M Freichel
    Institut fur Pharmakologie und Toxikologie, Universitat des Saarlandes, D 66421 Homburg, Germany
    Nat Cell Biol 3:121-7. 2001

Detail Information

Publications118 found, 100 shown here

  1. ncbi request reprint International Union of Pharmacology. XLII. Compendium of voltage-gated ion channels: cyclic nucleotide-modulated channels
    Franz Hofmann
    Institut fur Pharmakologie und Toxikologie, Technischen Universitat Munchen, Biedersteiner Strasse 29, D 80802 Munich, Germany
    Pharmacol Rev 55:587-9. 2003
    ..The complete Compendium, including data tables for each member of the cyclic nucleotide-modulated channel family can be found at http://www.iuphar-db.org/iuphar-ic/...
  2. ncbi request reprint Function of cGMP-dependent protein kinases as revealed by gene deletion
    F Hofmann
    Institut für Pharmakologie und Toxicologie, Technische Universitat Munchen, Biedersteiner Strasse 29, D 80802 Munich, Germany
    Physiol Rev 86:1-23. 2006
    ..These data show that cGKs are signaling molecules involved in many biological functions...
  3. ncbi request reprint International Union of Pharmacology. LI. Nomenclature and structure-function relationships of cyclic nucleotide-regulated channels
    Franz Hofmann
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner StraBe 29, D 80802 München, Germany
    Pharmacol Rev 57:455-62. 2005
  4. ncbi request reprint The role of subunit composition on prepulse facilitation of the cardiac L-type calcium channel
    S Dai
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Munich, Germany
    FEBS Lett 442:70-4. 1999
    ..We conclude that the expressed alpha1C-a beta2a complex is sufficient to support prepulse facilitation. Facilitation is prevented by coexpression of the alpha2delta subunit...
  5. ncbi request reprint Distribution of IRAG and cGKI-isoforms in murine tissues
    A Geiselhöringer
    Institut für Pharmakologie und Toxikologie der TU München, Biedersteiner Strasse 29, 80802 Munchen, Germany
    FEBS Lett 575:19-22. 2004
    ..These results suggest that IRAG may be involved in cGKI-dependent and -independent pathways...
  6. pmc Two pacemaker channels from human heart with profoundly different activation kinetics
    A Ludwig
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    EMBO J 18:2323-9. 1999
    ..hHCN2 and hHCN4 currents differ profoundly from each other in their activation kinetics, being fast and slow, respectively. We thus conclude that hHCN2 and hHCN4 may underlie the fast and slow component of cardiac If, respectively...
  7. pmc IRAG is essential for relaxation of receptor-triggered smooth muscle contraction by cGMP kinase
    Angela Geiselhöringer
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    EMBO J 23:4222-31. 2004
    ..We conclude that cGMP-dependent relaxation of hormone receptor-triggered smooth muscle contraction essentially depends on the interaction of cGKI-IRAG with IP(3)RI...
  8. ncbi request reprint Cellular expression and functional characterization of four hyperpolarization-activated pacemaker channels in cardiac and neuronal tissues
    S Moosmang
    , , Germany
    Eur J Biochem 268:1646-52. 2001
    ..Our results suggest that the heterogeneity of native I(h) currents is generated, at least in part, by the tissue-specific expression of HCN channel genes...
  9. doi request reprint Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle
    Eva Frei
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Munich, Germany
    Am J Physiol Gastrointest Liver Physiol 297:G834-9. 2009
    ....
  10. ncbi request reprint Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4-/- mice
    M Freichel
    Institut fur Pharmakologie und Toxikologie, Universitat des Saarlandes, D 66421 Homburg, Germany
    Nat Cell Biol 3:121-7. 2001
    ....
  11. ncbi request reprint A family of hyperpolarization-activated mammalian cation channels
    A Ludwig
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Germany
    Nature 393:587-91. 1998
    ..We have also identified full-length sequences of two related channels, HAC2 and HAC3, that are specifically expressed in the brain, indicating the existence of a family of hyperpolarization-activated cation channels...
  12. ncbi request reprint Absence of the gamma subunit of the skeletal muscle dihydropyridine receptor increases L-type Ca2+ currents and alters channel inactivation properties
    D Freise
    Institut fur Pharmakologie und Toxikologie, Universitat des Saarlandes, D 66421 Homburg, Germany
    J Biol Chem 275:14476-81. 2000
    ..These results indicate that gamma1 decreases the amount of Ca(2+) entry during stimulation of skeletal muscle...
  13. ncbi request reprint Functional embryonic cardiomyocytes after disruption of the L-type alpha1C (Cav1.2) calcium channel gene in the mouse
    C Seisenberger
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Strasse 29, D 80802 München, Germany
    J Biol Chem 275:39193-9. 2000
    ..3) knockout embryos at day 12.5 p.c. These results indicate that, up to day 14 p.c., contraction of murine embryonic hearts requires an unidentified, low affinity L-type like calcium channel...
  14. ncbi request reprint A T-type calcium channel from mouse brain
    N Klugbauer
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Str 29, D 80802 Munich, Germany
    Pflugers Arch 437:710-5. 1999
    ..The kinetics of ICa inactivation were significantly slower than those of IBa. The expressed alpha1G channel has a relatively high sensitivity to mibefradil, but is only slightly affected by Ni2+...
  15. doi request reprint Phospholipase C mediated Ca2+ signals in murine urinary bladder smooth muscle
    Eva Frei
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Str 29, D 80802 München, Germany
    Eur J Pharmacol 610:106-9. 2009
    ..The results show that PLC/IP(3)-dependent Ca(2+) release is activated by stimulation with carbachol in urinary bladder smooth muscle but has a minor contribution to overall carbachol-induced Ca(2+) signals...
  16. ncbi request reprint Enhanced expression of L-type Cav1.3 calcium channels in murine embryonic hearts from Cav1.2-deficient mice
    Man Xu
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    J Biol Chem 278:40837-41. 2003
    ..2 (-/-) cardiomyocytes. In summary, our results imply that calcium channel expression is dynamically regulated during heart development and that the Cav1.3 channel may substitute for Cav1.2 during early embryogenesis...
  17. ncbi request reprint Long-term potentiation in the hippocampal CA1 region of mice lacking cGMP-dependent kinases is normal and susceptible to inhibition of nitric oxide synthase
    T Kleppisch
    Institut fur Pharmakologie und Toxikologie, 80802 Munchen, Germany
    J Neurosci 19:48-55. 1999
    ..These results show that cGKs are not involved in LTP in mice and that NO induces LTP through an alternative cGMP-independent pathway, possibly ADP-ribosylation...
  18. ncbi request reprint Primary structure and functional expression of a cyclic nucleotide-gated channel from rabbit aorta
    M Biel
    Institut fur Pharmakologie und Toxikologie, Technischen Universitat Munchen, Germany
    FEBS Lett 329:134-8. 1993
    ..The complete amino acid sequence of a CNG channel from rabbit aorta has been deduced by cloning and sequence analysis of the cDNA. Synthetic RNA derived from this cDNA induces the formation of a functional CNG channel in Xenopus oocytes...
  19. pmc A proatherogenic role for cGMP-dependent protein kinase in vascular smooth muscle cells
    Wiebke Wolfsgruber
    Institut fur Pharmakologie und Toxikologie, Technische Universitat, Biedersteiner Strasse 29, 80802 Munich, Germany
    Proc Natl Acad Sci U S A 100:13519-24. 2003
    ..We suggest that activation of smooth muscle cGKI contributes to the proatherogenic effect of NO and that inhibition of cGKI might be a therapeutic option for treating atherosclerosis in humans...
  20. ncbi request reprint Pacemaker channels and sinus node arrhythmia
    Juliane Stieber
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Munich, Germany
    Trends Cardiovasc Med 14:23-8. 2004
    ....
  21. ncbi request reprint Molecular determinants of the interaction between the inositol 1,4,5-trisphosphate receptor-associated cGMP kinase substrate (IRAG) and cGMP kinase Ibeta
    A Ammendola
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, D 80802 München, Germany
    J Biol Chem 276:24153-9. 2001
    ..These results show that cGMP induced reduction of cytosolic calcium concentrations requires cGKIbeta and phosphorylation of Ser(696) of IRAG...
  22. ncbi request reprint Distribution of cGMP-dependent protein kinase type I and its isoforms in the mouse brain and retina
    S Feil
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    Neuroscience 135:863-8. 2005
    ....
  23. pmc An isoform of the rod photoreceptor cyclic nucleotide-gated channel beta subunit expressed in olfactory neurons
    A Sautter
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munich, Germany
    Proc Natl Acad Sci U S A 95:4696-701. 1998
    ..The expression of various CNG4 isoforms in retina and olfactory epithelium indicates that the CNG4 subunit may be necessary for normal function of both photoreceptor and olfactory CNG channels...
  24. doi request reprint Signaling through cGMP-dependent protein kinase I in the amygdala is critical for auditory-cued fear memory and long-term potentiation
    Cindy Paul
    Institut fur Pharmakologie und Toxikologie, 80802 Munich, Germany
    J Neurosci 28:14202-12. 2008
    ..In contrast, cGKII null mutants lack these phenotypes. Our data suggest a function of cGKI, likely the beta isoform, in the LA, supporting synaptic plasticity and consolidation of fear memory...
  25. ncbi request reprint Temporally controlled somatic mutagenesis in smooth muscle
    S Kühbandner
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Munchen, Germany
    Genesis 28:15-22. 2000
    ..The recombined genome persisted for at least four months after tamoxifen treatment. SM-CreER(T2)(ki) transgenic mice should be useful to study the effects of various somatic mutations in smooth muscle...
  26. pmc Another member of the cyclic nucleotide-gated channel family, expressed in testis, kidney, and heart
    M Biel
    Institut fur Pharmakologie und Toxikologie, Technischen Universitat Munchen, Germany
    Proc Natl Acad Sci U S A 91:3505-9. 1994
    ..It is likely that CNG-3 protein is responsible for cGMP-induced Ca2+ entry in cells other than sensory cells...
  27. ncbi request reprint Reconstruction of the dihydropyridine site in a non-L-type calcium channel: the role of the IS6 segment
    L Lacinova
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Munich, Germany
    FEBS Lett 451:152-6. 1999
    ....
  28. pmc Absence of modulation of the expressed calcium channel alpha1G subunit by alpha2delta subunits
    L Lacinova
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    J Physiol 516:639-45. 1999
    ..No significant difference was found for any of the examined parameters. 3. These results suggest that the LVA alpha1G channel is not regulated by known auxiliary alpha2delta subunits...
  29. pmc Defective smooth muscle regulation in cGMP kinase I-deficient mice
    A Pfeifer
    Institut fur Pharmakologie und Toxikologie, Munchen, Biedersteiner, Str 29, 80802 Müchen, Germany
    EMBO J 17:3045-51. 1998
    ..However, cGKI-deficient smooth muscle responded normally to cAMP, indicating that cAMP and cGMP signal via independent pathways, with cGKI being the specific mediator of the NO/cGMP effects in murine smooth muscle...
  30. ncbi request reprint The structure of the murine calcium channel gamma-subunit gene and protein
    U Wissenbach
    Institut fur Pharmakologie und Toxikologie, Universitat des Saarlandes, Homburg, Germany
    Biol Chem 379:45-50. 1998
    ....
  31. ncbi request reprint The biology of cyclic GMP-dependent protein kinases
    Franz Hofmann
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, D 80802 München, Germany
    J Biol Chem 280:1-4. 2005
  32. pmc Facilitation of murine cardiac L-type Ca(v)1.2 channel is modulated by calmodulin kinase II-dependent phosphorylation of S1512 and S1570
    Anne Blaich
    Forschergruppe 923, Munich Center for Integrated Protein Science, and Institut für Pharmakologie und Toxikologie, Technische Universitat Munchen, 80802 Munich, Germany
    Proc Natl Acad Sci U S A 107:10285-9. 2010
    ..Our results strongly support the notion that CaMKII-dependent phosphorylation of Cav1.2 at S1512 and S1570 mediates Ca(2+) current facilitation in the murine heart...
  33. doi request reprint cGMP-dependent protein kinase type I promotes CREB/CRE-mediated gene expression in neurons of the lateral amygdala
    Cindy Paul
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    Neurosci Lett 473:82-6. 2010
    ..In summary, we provide evidence that cytosolic cGKI can support fear memory consolidation and LTP in neurons of the lateral amygdala via activation of CREB and CRE-dependent transcription...
  34. pmc Selective loss of cone function in mice lacking the cyclic nucleotide-gated channel CNG3
    M Biel
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, 80802 Munich, Germany
    Proc Natl Acad Sci U S A 96:7553-7. 1999
    ..CNG3-deficient mice provide an animal model to dissect unequivocally the contribution of rod and cone pathways for normal retinal function...
  35. ncbi request reprint Analysis of calcium channels by conditional mutagenesis
    S Moosmang
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Str 29, 80802 Munchen, Germany
    Handb Exp Pharmacol . 2007
    ....
  36. pmc Impairment of LTD and cerebellar learning by Purkinje cell-specific ablation of cGMP-dependent protein kinase I
    Robert Feil
    Institut fur Pharmakologie und Toxikologie, Technische Universitat, Biedersteiner Str 29, D 80802 München, Germany
    J Cell Biol 163:295-302. 2003
    ..These results indicate that cGKI in PCs is dispensable for general motor coordination, but that it is required for cerebellar LTD and specific forms of motor learning, namely the adaptation of the VOR...
  37. ncbi request reprint Identification and functional characterization of a calcium channel gamma subunit
    R Eberst
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, D 80802 München, Germany
    Pflugers Arch 433:633-7. 1997
    ....
  38. ncbi request reprint Inhibition of L-type Cav1.2 Ca2+ channels by 2,(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002) and 2-[1-(3-dimethyl-aminopropyl)-5-methoxyindol-3-yl]-3-(1H-indol-3-yl) maleimide (Go6983)
    A Welling
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Str 29, 80802 Munchen, Germany
    Mol Pharmacol 67:541-4. 2005
    ..Because both drugs are commonly used at concentrations of approximately 10 microM or higher, the interpretation of such experiments is questionable with respect to a regulatory action of PI3-K or PKC on L-type Ca(2+) channels...
  39. pmc Cardiac hypertrophy is not amplified by deletion of cGMP-dependent protein kinase I in cardiomyocytes
    Robert Lukowski
    Forschergruppe 923 and Institut für Pharmakologie und Toxikologie, Technische Universitat Munchen, D 80802 Munich, Germany
    Proc Natl Acad Sci U S A 107:5646-51. 2010
    ..These results strongly suggest that cardiac myocyte cGKI does not affect the development of heart hypertrophy induced by pressure overload or chronic ISO infusion...
  40. ncbi request reprint Calmodulin kinase II is involved in voltage-dependent facilitation of the L-type Cav1.2 calcium channel: Identification of the phosphorylation sites
    Tae Seong Lee
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Munchen, Germany
    J Biol Chem 281:25560-7. 2006
    ..2 protein was strongly decreased in the S1512A/S1570A double mutant. These results suggest that voltage-dependent facilitation of the Ca(v)1.2 channel depends on the phosphorylation of Ser1512/Ser1570 by calmodulin kinase II...
  41. pmc Unchanged beta-adrenergic stimulation of cardiac L-type calcium channels in Ca v 1.2 phosphorylation site S1928A mutant mice
    Toni Lemke
    Institut fur Pharmakologie, Technische Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    J Biol Chem 283:34738-44. 2008
    ..2 by PKA. Therefore, our data show that PKA phosphorylation of Ser(1928) of Ca(v)1.2 is not functionally involved in beta-adrenergic stimulation of Ca(v)1.2-mediated Ca(2+) influx into the cardiomyocyte...
  42. doi request reprint A complex of Ca(V)1.2/PKC is involved in muscarinic signaling in smooth muscle
    Maria Huster
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Str 29, 80802 Munich, Germany
    FASEB J 24:2651-9. 2010
    ..2 is a substrate for PKC-mediated phosphorylation. These findings suggest that a signaling complex consisting of Ca(V)1.2, PKC, and, probably, PLD controls muscarinic-mediated phasic contraction of urinary bladder smooth muscle...
  43. doi request reprint cGMP-dependent protein kinase II and aldosterone secretion
    Beate Spiessberger
    Institut fur Pharmakologie und Toxikologie, Medizinische Fakultat, Technische Universitat Munchen, Germany
    FEBS J 276:1007-13. 2009
    ..These results support the view that cGKII modulates aldosterone secretion in the murine adrenal cortex...
  44. ncbi request reprint Neutrophil dysfunction in guanosine 3',5'-cyclic monophosphate-dependent protein kinase I-deficient mice
    Claudia G Werner
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Munchen, Germany
    J Immunol 175:1919-29. 2005
    ..In summary, these results show that cGKIalpha down-regulates Ca2+ transients and chemotaxis in murine neutrophils. The regulatory influences of cGKIalpha on the secretagogue responses are complex, depending on the granule subtype...
  45. ncbi request reprint cGMP signals mainly through cAMP kinase in permeabilized murine aorta
    René Wörner
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Str 29, 80802 Munchen, Germany
    Am J Physiol Heart Circ Physiol 292:H237-44. 2007
    ..The effect of 8-Br-cGMP on U-46619-induced contraction was attenuated by PKI 5-24. These results show that cGKI has only a small inhibitory effect on Ca(2+) sensitization in murine aortas...
  46. ncbi request reprint Primary structure of a novel ABC transporter with a chromosomal localization on the band encoding the multidrug resistance-associated protein
    N Klugbauer
    Institut fur Pharmakologie und Toxikologie, Technischen Universitat Munchen, Germany
    FEBS Lett 391:61-5. 1996
    ..The genomic clustering of both transporters, typical also for other members of the ABC family, supports the notion that ABC-C may be involved in development of resistance to xenobiotics...
  47. ncbi request reprint Identification of the amino acid sequences responsible for high affinity activation of cGMP kinase Ialpha
    P Ruth
    Institut für Pharmakologie und Toxikologie der Technische Universität München, Biedersteiner Strasse 29, D 80802 München, Germany
    J Biol Chem 272:10522-8. 1997
    ....
  48. ncbi request reprint Regulation of the calcium channel alpha(1G) subunit by divalent cations and organic blockers
    L Lacinova
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Str 29, 80802, Munchen, Germany
    Neuropharmacology 39:1254-66. 2000
    ..The alpha(1G) current was minimally activated by 1 microM Bay K 8644...
  49. ncbi request reprint Low voltage activated calcium channels: from genes to function
    L Lacinova
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Germany
    Gen Physiol Biophys 19:121-36. 2000
    ..In contrast, alpha2delta-1, alpha2delta-3, gamma-2 and gamma-4 subunits failed to modulate the current or had only minor effects...
  50. ncbi request reprint A family of gamma-like calcium channel subunits
    N Klugbauer
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Str 29, 80802, Munich, Germany
    FEBS Lett 470:189-97. 2000
    ..The coexpression of the gamma-5 subunit accelerates the time course of current activation and inactivation of the alpha(1G) T-type calcium channel...
  51. ncbi request reprint Structure and function of cardiac pacemaker channels
    A Ludwig
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Munchen, Deutschland
    Cell Physiol Biochem 9:179-86. 1999
    ..The relationships between cloned HCN channel types and native cardiac I(f) currents are explored...
  52. ncbi request reprint Isradipine interacts with the open state of the L-type calcium channel at high concentrations
    L Lacinova
    Institut für Pharmakologie and Toxikologie TU München, Germany
    Receptors Channels 6:153-64. 1998
    ..These results demonstrate that the mutations of Ch30 affect selectively the high affinity inhibition of an inactivated channel and unmask a low affinity interaction of isradipine with an open state of the channel...
  53. ncbi request reprint State- and isoform-dependent interaction of isradipine with the alpha1C L-type calcium channel
    L Lacinova
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Munich, Germany
    Pflugers Arch 440:50-60. 2000
    ..Our results suggest that the higher DHP selectivity of the alpha1Cb versus the alpha1Ca channel is caused by the structural difference in the binding site and not by different transitions between resting, open and inactivated states...
  54. ncbi request reprint SM22alpha modulates vascular smooth muscle cell phenotype during atherogenesis
    Susanne Feil
    Institut fur Pharmakologie und Toxikologie, Technische Universitat, Biedersteiner Str 29, 80802 Munchen, Germany
    Circ Res 94:863-5. 2004
    ..These results identify a role for SM22alpha in the regulation of SMC phenotype during atherogenesis...
  55. ncbi request reprint Antihypertensive effects of the putative T-type calcium channel antagonist mibefradil are mediated by the L-type calcium channel Cav1.2
    Sven Moosmang
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Germany
    Circ Res 98:105-10. 2006
    ..2SMAKO mice. These results demonstrate that the effect of the putative T-type Ca2+ channel-selective blocker mibefradil on blood pressure and small vessel myogenic tone is mediated by the Cav1.2 L-type Ca2+ channel...
  56. ncbi request reprint An essential role of Cav1.2 L-type calcium channel for urinary bladder function
    Jörg W Wegener
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Str 29, D 80802 München, Germany
    FASEB J 18:1159-61. 2004
    ..These results show that the Cav1.2 Ca2+ channel is essential for normal bladder function. The Rho-kinase and Ca2+-release pathways cannot compensate the lack of the L-type Ca2+ channel...
  57. ncbi request reprint Functional expression of the human HCN3 channel
    Juliane Stieber
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    J Biol Chem 280:34635-43. 2005
    ..The missing response to cAMP distinguishes human HCN3 from both the well cAMP responding HCN subtypes 2 and 4 and the weak responding subtype 1...
  58. ncbi request reprint Mouse models to study L-type calcium channel function
    Sven Moosmang
    Institut fur Pharmakologie, Technische Universitat Munchen, Biedersteiner Strasse 29, 80802, Munchen, Germany
    Pharmacol Ther 106:347-55. 2005
    ....
  59. ncbi request reprint cGMP-dependent protein kinases in drug discovery
    Jens Schlossmann
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    Drug Discov Today 10:627-34. 2005
    ..Isozyme-specific inhibitors and activators of cGK and its downstream substrates might act more specifically than upstream signalling activators, such as organic nitrates and phosphodiesterase inhibitors...
  60. ncbi request reprint Function of cGMP-dependent protein kinases in the nervous system
    Robert Feil
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Munchen, Germany
    Rev Neurosci 16:23-41. 2005
    ..Thus, cGKs serve key functions in the transduction of cGMP signals into cellular responses in distinct regions of the nervous system.-..
  61. ncbi request reprint Bradycardic and proarrhythmic properties of sinus node inhibitors
    Juliane Stieber
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Str 29, 80802 Munchen, Germany
    Mol Pharmacol 69:1328-37. 2006
    ..Hence, all available sinus node inhibitors possess an as-yet-unrecognized proarrhythmic potential...
  62. ncbi request reprint A heretical view on the role of NO and cGMP in vascular proliferative diseases
    Robert Feil
    Institut fur Pharmakologie und Toxikologie, Technische Universitat, Biedersteiner Strasse 29, 80802 Munchen, Germany
    Trends Mol Med 11:71-5. 2005
    ..This new concept has important implications for the use of cGMP-elevating drugs in humans and might help to identify novel therapeutic strategies for vascular proliferative diseases...
  63. ncbi request reprint Importance of NO/cGMP signalling via cGMP-dependent protein kinase II for controlling emotionality and neurobehavioural effects of alcohol
    Claudia Werner
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    Eur J Neurosci 20:3498-506. 2004
    ..No taste differences to sweet solutions were observed compared to control animals. In summary, our data show that cGKII activity modulates anxiety-like behaviour and neurobehavioural effects of alcohol...
  64. ncbi request reprint Role of hippocampal Cav1.2 Ca2+ channels in NMDA receptor-independent synaptic plasticity and spatial memory
    Sven Moosmang
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, 80802 Munich, Germany
    J Neurosci 25:9883-92. 2005
    ..2 channel-mediated Ca2+ influx to either process...
  65. ncbi request reprint Calcium channel alpha2delta subunits: differential expression, function, and drug binding
    Norbert Klugbauer
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Str 29, 80802 Munchen, Germany
    J Bioenerg Biomembr 35:639-47. 2003
    ..Alpha2delta subunits can also support pharmacological interactions with drugs that are used for the treatment of epilepsy and neuropathic pain...
  66. ncbi request reprint Control of intestinal motility by the Ca(v)1.2 L-type calcium channel in mice
    Jörg W Wegener
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Str 29, München 80802, Germany
    FASEB J 20:1260-2. 2006
    ..In conclusion, the Ca(v)1.2 channel is essential for electromechanical coupling and important for pharmaco-mechanical coupling in intestinal smooth muscle and cannot be substituted functionally by other Ca2+ entry pathways...
  67. ncbi request reprint Functional reconstitution of vascular smooth muscle cells with cGMP-dependent protein kinase I isoforms
    Robert Feil
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Universitat Munchen, Munchen, Germany
    Circ Res 90:1080-6. 2002
    ..These results suggest that one component of NO/cGMP-induced smooth muscle relaxation is the activation of the cGKIalpha isoform, which decreases the noradrenaline-stimulated cytosolic Ca(2+) level...
  68. ncbi request reprint Association of phospholamban with a cGMP kinase signaling complex
    Angela Koller
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Strasse 29, 80802 Munich, Germany
    Biochem Biophys Res Commun 300:155-60. 2003
    ..PLB, IP(3)RI, and cGMP kinase Ibeta were co-immune precipitated after expression in COS-7 cells. These results suggest that PLB may have additional functions to regulate the activity of SERCA II...
  69. pmc Absence epilepsy and sinus dysrhythmia in mice lacking the pacemaker channel HCN2
    Andreas Ludwig
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, D 80802 München, Germany
    EMBO J 22:216-24. 2003
    ..Our results define the physiological role of the HCN2 subunit as a major determinant of membrane resting potential that is required for regular cardiac and neuronal rhythmicity...
  70. ncbi request reprint Hippocampal cGMP-dependent protein kinase I supports an age- and protein synthesis-dependent component of long-term potentiation but is not essential for spatial reference and contextual memory
    Thomas Kleppisch
    Institut fur Pharmakologie und Toxikologie, 80802 Munchen, Germany, Max Planck Institut fur Psychiatrie, 80804 München, Germany
    J Neurosci 23:6005-12. 2003
    ..These results suggest that hippocampal cGKI supports an age- and protein synthesis-dependent form of hippocampal LTP, whereas it is dispensable for hippocampus-dependent spatial reference and contextual memory...
  71. ncbi request reprint Signaling through NO and cGMP-dependent protein kinases
    Jens Schlossmann
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Germany
    Ann Med 35:21-7. 2003
    ..Activation of the NO/cGMP/cGKI pathway induces relaxation of smooth muscle by lowering the cytosolic calcium level and/or by calcium desensitization of the contractile elements...
  72. ncbi request reprint Insights into cGMP signalling derived from cGMP kinase knockout mice
    Jens Schlossmann
    Institute for Pharmacology and Toxicology, Technical University Munich, Biedersteiner Strasse 29, 80802 Munich, Germany
    Front Biosci 10:1279-89. 2005
    ..cGKII is involved in intestinal water secretion, bone growth and circardian rhythmicity. The cGK mutant mice are important tools to obtain detailed insights into cGMP-mediated signalling pathways in health and disease...
  73. pmc Cyclic guanosine monophosphate-dependent protein kinase I promotes adhesion of primary vascular smooth muscle cells
    Pascal Weinmeister
    Institut fur Pharmakologie und Toxikologie, Technischen Universiät München, D 80802 München, Germany
    Mol Biol Cell 19:4434-41. 2008
    ..Together, these results identify a novel proadhesive function of cGMP/cGKI signaling in primary VSMCs and suggest that the opposing effects of this pathway on VSMC number depend on the phenotypic context of the cells...
  74. ncbi request reprint L-type Ca2+ channels of the embryonic mouse heart
    Norbert Klugbauer
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Str 29, 80802 Munich, Germany
    Eur J Pharmacol 447:279-84. 2002
    ..3 channel has a similar affinity for nisoldipine as Ca(v)1.2+/+ cardiomyocytes, the L-type-like Ca(2+) channel found in Ca(v)1.2+/+ and -/- cardiomyocytes is not identical with the new Ca(v)1.3 splice variants...
  75. ncbi request reprint Molecular basis for the different activation kinetics of the pacemaker channels HCN2 and HCN4
    Juliane Stieber
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munich, Germany
    J Biol Chem 278:33672-80. 2003
    ..Taken together our results indicate that the speed of activation of the HCN4 channel is determined by structural elements present in the S1, S1-S2 linker, and the S2 segment...
  76. pmc Role of smooth muscle cGMP/cGKI signaling in murine vascular restenosis
    Robert Lukowski
    Institut für Pharmakologie und Toxikologie der TUM, Biedersteiner Str 29, D 80802 München, Germany
    Arterioscler Thromb Vasc Biol 28:1244-50. 2008
    ..The purpose of this study was to examine the functional role of the smooth muscle cGMP/cGKI signaling cascade in restenosis of vessels...
  77. ncbi request reprint Hypothyroidism does not affect the dihydropyridine sensitivity of precontracted murine uterus
    Jörg W Wegener
    Institute of Pharmacology and Toxicology, Technische Universitat Munchen, Biedersteiner Strasse 29, 80202 Munich, Germany
    Can J Physiol Pharmacol 81:890-3. 2003
    ..These results demonstrate that hypothyroidism does not change dihydropyridine sensitivity (i.e., the pattern of Ca2+-channel expression) in the murine uterus...
  78. pmc The hyperpolarization-activated channel HCN4 is required for the generation of pacemaker action potentials in the embryonic heart
    Juliane Stieber
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteinerstrasse 29, D 80802 Munich, Germany
    Proc Natl Acad Sci U S A 100:15235-40. 2003
    ..0, were not detected in HCN4-deficient embryos. Thus, HCN4 channels are essential for the proper generation of pacemaker potentials in the emerging sinoatrial node...
  79. pmc Dominant role of smooth muscle L-type calcium channel Cav1.2 for blood pressure regulation
    Sven Moosmang
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Strasse 29, D 80802 München, Germany
    EMBO J 22:6027-34. 2003
    ..2 channel. These results show that Cav1.2 calcium channels are key players in the hormonal regulation of blood pressure and development of myogenic tone...
  80. ncbi request reprint Rising behind NO: cGMP-dependent protein kinases
    F Hofmann
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Str 29, D 80802 München, Germany tu muenchen de
    J Cell Sci 113:1671-6. 2000
    ..cGKII regulates in vivo intestinal fluid secretion by phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR), bone growth and renal renin secretion by phosphorylation of unknown proteins...
  81. ncbi request reprint Expression of cyclic nucleotide-gated cation channels in non-sensory tissues and cells
    M Distler
    Institut fur Pharmakologie und Toxikologie, Technischen Universitat Munchen, Germany
    Neuropharmacology 33:1275-82. 1994
    ..These data suggest that some of the effects of cGMP in peripheral tissues and cells might involve the activation of CNG channels...
  82. pmc Calcium channel beta subunit heterogeneity: functional expression of cloned cDNA from heart, aorta and brain
    R Hullin
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, FRG
    EMBO J 11:885-90. 1992
    ..These results together with the known alpha 1 subunit heterogeneity, suggest that different types of calcium currents may depend on channel subunit composition...
  83. ncbi request reprint Cyclic GMP-dependent protein kinases and the cardiovascular system: insights from genetically modified mice
    Robert Feil
    Institut fur Pharmakologie und Toxikologie, Technische Universitat, Biedersteiner Str 29, D 80802 München, Germany
    Circ Res 93:907-16. 2003
    ..Thus, cGKs may represent novel drug targets for the treatment of human cardiovascular disorders...
  84. ncbi request reprint Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II
    A Pfeifer
    Institut f ur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, D 80802 M unchen, Germany
    Science 274:2082-6. 1996
    ..The cGKII-deficient mice also developed dwarfism that was caused by a severe defect in endochondral ossification at the growth plates. These results indicate that cGKII plays a central role in diverse physiological processes...
  85. pmc Characterization of voltage-dependent sodium and calcium channels in mouse pancreatic A- and B-cells
    Sheila Vignali
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Strasse 29, D 80802 München, Germany
    J Physiol 572:691-706. 2006
    ..Instead, two TTX-sensitive sodium currents were found: an early inactivating and a residual current. The residual current was only recovered in a subpopulation of B-cells. A putative genetic background for these currents is Na(v)1.7...
  86. pmc Impaired insulin secretion and glucose tolerance in beta cell-selective Ca(v)1.2 Ca2+ channel null mice
    Verena Schulla
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Strasse 29, D 80802 München, Germany
    EMBO J 22:3844-54. 2003
    ..2 channel. Thus, the conduit of Ca(2+) entry determines the ability of the cation to elicit secretion...
  87. doi request reprint Combined reporter gene PET and iron oxide MRI for monitoring survival and localization of transplanted cells in the rat heart
    Takahiro Higuchi
    Nuklearmedizinische Klinik und Poliklinik, Technische Universitat Munchen, Munich, Germany
    J Nucl Med 50:1088-94. 2009
    ..This study assessed the feasibility and usefulness of combined PET and MRI for monitoring cell engraftment and survival after cell transplantation...
  88. ncbi request reprint Abnormal neocortical development in mice lacking cGMP-dependent protein kinase I
    Galina P Demyanenko
    Department of Biochemistry and Biophysics CB 7260, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Brain Res Dev Brain Res 160:1-8. 2005
    ..These results demonstrate a role for cGKI signaling in cortical development related to neuronal migration/positioning that is important for dendritic orientation and connectivity...
  89. ncbi request reprint entla, a novel epileptic and ataxic Cacna2d2 mutant of the mouse
    Julia Brill
    Institut fur Biochemie, Emil Fischer Zentrum, Friedrich Alexander Universitat Erlangen Nurnberg, D 91054 Erlangen, Germany
    J Biol Chem 279:7322-30. 2004
    ..entla is allelic to ducky and ducky2J, representing the third murine Cacna2d2 allele identified and so far the only one encoding an untruncated protein that is incorporated into membranes...
  90. ncbi request reprint Cav1.2 calcium channels modulate the spiking pattern of hippocampal pyramidal cells
    Lubica Lacinova
    Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34 Bratislava, Slovakia
    Life Sci 82:41-9. 2008
    ..2 channels are involved in regulating the intrinsic excitability of CA1 pyramidal neurons. This cellular mechanism may contribute to the known function of Ca(v)1.2 channels in supporting synaptic plasticity and memory...
  91. ncbi request reprint Individual cerebellar Purkinje cells express different cGMP phosphodiesterases (PDEs): in vivo phosphorylation of cGMP-specific PDE (PDE5) as an indicator of cGMP-dependent protein kinase (PKG) activation
    Masami Shimizu-Albergine
    Department of Pharmacology, University of Washington, Seattle, Washington 98195 7280, USA
    J Neurosci 23:6452-9. 2003
    ....
  92. pmc Phosphorylation of GSK-3beta by cGMP-dependent protein kinase II promotes hypertrophic differentiation of murine chondrocytes
    Yosuke Kawasaki
    Sensory and Motor System Medicine, Faculty of Medicine, University of Tokyo, Tokyo, Japan
    J Clin Invest 118:2506-15. 2008
    ..These data indicate that hypertrophic differentiation of growth plate chondrocytes during skeletal growth is promoted by phosphorylation and inactivation of GSK-3beta by cGKII...
  93. ncbi request reprint Autophosphorylation of cGMP-dependent protein kinase type II
    Arie B Vaandrager
    Department of Biochemistry, Erasmus University Medical Center Rotterdam 3000 DR, The Netherlands
    J Biol Chem 278:28651-8. 2003
    ....
  94. pmc Significance and therapeutic potential of the natriuretic peptides/cGMP/cGMP-dependent protein kinase pathway in vascular regeneration
    Kenichi Yamahara
    Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 100:3404-9. 2003
    ....
  95. pmc Direct activation of PDE5 by cGMP: long-term effects within NO/cGMP signaling
    Florian Mullershausen
    Abteilung für Pharmakologie und Toxikologie, Medizinische Fakultat, Ruhr Universitat Bochum, MA N1 43, Universitaetsstr 150, 44780 Bochum, Germany
    J Cell Biol 160:719-27. 2003
    ..In sum, we suggest NO-induced activation and phosphorylation of PDE5 as the mechanism for a long-lasting negative feedback loop shaping the cGMP response in human platelets in order to adapt to the amount of NO available...
  96. ncbi request reprint A stimulatory role for cGMP-dependent protein kinase in platelet activation
    Zhenyu Li
    Department of Pharmacology, College of Medicine, University of Illinois, 835 South Wolcott Avenue, Chicago, IL 60612, USA
    Cell 112:77-86. 2003
    ..The cGMP-stimulated platelet responses are biphasic, consisting of an initial transient stimulatory response that promotes platelet aggregation and a subsequent inhibitory response that limits the size of thrombi...
  97. pmc cGMP-mediated signaling via cGKIalpha is required for the guidance and connectivity of sensory axons
    Hannes Schmidt
    Developmental Neurobiology Group, Medical Research Council, Max Delbruck Centrum fur Molekulare Medizin, Robert Rossle Strasse 10, D 13092 Berlin, Germany
    J Cell Biol 159:489-98. 2002
    ..Our studies therefore reveal that cGMP signaling is important for axonal growth in vivo and in vitro...
  98. ncbi request reprint Cyclic GMP-dependent protein kinase II plays a critical role in C-type natriuretic peptide-mediated endochondral ossification
    Takashi Miyazawa
    Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Sakyo ku, Kyoto 606 8507, Japan
    Endocrinology 143:3604-10. 2002
    ..This study provides in vivo and in vitro genetic evidence that cGKII plays a critical role in CNP-mediated endochondral ossification...
  99. ncbi request reprint Cilnidipine is a novel slow-acting blocker of vascular L-type calcium channels that does not target protein kinase C
    Matthias Löhn
    Franz Volhard Clinic, Humboldt University of Berlin, Germany
    J Hypertens 20:885-93. 2002
    ....
  100. ncbi request reprint cGMP-dependent protein kinase I mediates the negative inotropic effect of cGMP in the murine myocardium
    Jörg W Wegener
    Pharmakologisches Institut, Universitat Mainz, Germany
    Circ Res 90:18-20. 2002
    ..These results demonstrate that cGKI mediates the negative inotropic effect of cGMP in the myocardium of juvenile and adult mice...
  101. ncbi request reprint Regulation of cGMP-specific phosphodiesterase (PDE5) phosphorylation in smooth muscle cells
    Sergei D Rybalkin
    Department of Pharmacology, University of Washington, Seattle, Washington 98195 7280, USA
    J Biol Chem 277:3310-7. 2002
    ..Together, the data suggest that phosphorylation and activation of PDE5 by PKG I and its subsequent dephosphorylation by myosin phosphatase may be key steps in the regulation of relaxation/contraction cycles of smooth muscle...