Ulrich Kück

Summary

Country: Germany

Publications

  1. pmc A mutant defective in sexual development produces aseptate ascogonia
    Sandra Bloemendal
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, ND7 131, Universitatsstrasse 150, 44780 Bochum, Germany
    Eukaryot Cell 9:1856-66. 2010
  2. pmc Combining laser microdissection and RNA-seq to chart the transcriptional landscape of fungal development
    Ines Teichert
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    BMC Genomics 13:511. 2012
  3. doi request reprint New tools for the genetic manipulation of filamentous fungi
    Ulrich Kück
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Universitatsstrasse 150, 44780 Bochum, Germany
    Appl Microbiol Biotechnol 86:51-62. 2010
  4. pmc A WD40 repeat protein regulates fungal cell differentiation and can be replaced functionally by the mammalian homologue striatin
    Stefanie Pöggeler
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, 44780 Bochum, Germany
    Eukaryot Cell 3:232-40. 2004
  5. pmc Two components of a velvet-like complex control hyphal morphogenesis, conidiophore development, and penicillin biosynthesis in Penicillium chrysogenum
    Birgit Hoff
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Universitatsstrasse 150, 44780 Bochum, Germany
    Eukaryot Cell 9:1236-50. 2010
  6. pmc Sexual reproduction and mating-type-mediated strain development in the penicillin-producing fungus Penicillium chrysogenum
    Julia Böhm
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, D 44780 Bochum, Germany
    Proc Natl Acad Sci U S A 110:1476-81. 2013
  7. pmc Members of the Penicillium chrysogenum velvet complex play functionally opposing roles in the regulation of penicillin biosynthesis and conidiation
    Katarina Kopke
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    Eukaryot Cell 12:299-310. 2013
  8. ncbi request reprint Versatile EGFP reporter plasmids for cellular localization of recombinant gene products in filamentous fungi
    Stefanie Pöggeler
    Department for General and Molecular Botany, Ruhr University Bochum, 44780 Bochum, Germany
    Curr Genet 43:54-61. 2003
  9. doi request reprint Evidence for Dicer-dependent RNA interference in the industrial penicillin producer Penicillium chrysogenum
    Danielle Janus
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat, Universitatsstr 150, 44780 Bochum, Germany
    Microbiology 155:3946-56. 2009
  10. pmc Three alpha-subunits of heterotrimeric G proteins and an adenylyl cyclase have distinct roles in fruiting body development in the homothallic fungus Sordaria macrospora
    Jens Kamerewerd
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    Genetics 180:191-206. 2008

Collaborators

Detail Information

Publications41

  1. pmc A mutant defective in sexual development produces aseptate ascogonia
    Sandra Bloemendal
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, ND7 131, Universitatsstrasse 150, 44780 Bochum, Germany
    Eukaryot Cell 9:1856-66. 2010
    ..This points to a specific role of PRO22 in the tubular and vesicular vacuolar network, and the loss of intercalary septation in ascogonia suggests that PRO22 functions during the initiation of sexual development...
  2. pmc Combining laser microdissection and RNA-seq to chart the transcriptional landscape of fungal development
    Ines Teichert
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    BMC Genomics 13:511. 2012
    ..Here, we used laser microdissection (LM) and RNA-seq to determine gene expression patterns in young fruiting bodies (protoperithecia) and non-reproductive mycelia of the ascomycete Sordaria macrospora...
  3. doi request reprint New tools for the genetic manipulation of filamentous fungi
    Ulrich Kück
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Universitatsstrasse 150, 44780 Bochum, Germany
    Appl Microbiol Biotechnol 86:51-62. 2010
    ....
  4. pmc A WD40 repeat protein regulates fungal cell differentiation and can be replaced functionally by the mammalian homologue striatin
    Stefanie Pöggeler
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, 44780 Bochum, Germany
    Eukaryot Cell 3:232-40. 2004
    ..Our data strongly suggest that an evolutionarily conserved cellular process controlling eukaryotic cell differentiation may regulate fruiting body formation...
  5. pmc Two components of a velvet-like complex control hyphal morphogenesis, conidiophore development, and penicillin biosynthesis in Penicillium chrysogenum
    Birgit Hoff
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Universitatsstrasse 150, 44780 Bochum, Germany
    Eukaryot Cell 9:1236-50. 2010
    ..chrysogenum, with structurally conserved components that have distinct developmental roles, illustrating the functional plasticity of these regulators in genera other than Aspergillus...
  6. pmc Sexual reproduction and mating-type-mediated strain development in the penicillin-producing fungus Penicillium chrysogenum
    Julia Böhm
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, D 44780 Bochum, Germany
    Proc Natl Acad Sci U S A 110:1476-81. 2013
    ..These discoveries of a sexual cycle and MAT gene function are likely to be of broad relevance for manipulation of other asexual fungi of economic importance...
  7. pmc Members of the Penicillium chrysogenum velvet complex play functionally opposing roles in the regulation of penicillin biosynthesis and conidiation
    Katarina Kopke
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    Eukaryot Cell 12:299-310. 2013
    ....
  8. ncbi request reprint Versatile EGFP reporter plasmids for cellular localization of recombinant gene products in filamentous fungi
    Stefanie Pöggeler
    Department for General and Molecular Botany, Ruhr University Bochum, 44780 Bochum, Germany
    Curr Genet 43:54-61. 2003
    ..In the latter, the heterologous egfp gene is stably inherited during meiotic divisions, as can easily be seen from fluorescent ascospores...
  9. doi request reprint Evidence for Dicer-dependent RNA interference in the industrial penicillin producer Penicillium chrysogenum
    Danielle Janus
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat, Universitatsstr 150, 44780 Bochum, Germany
    Microbiology 155:3946-56. 2009
    ..The applicability of the RNAi system in P. chrysogenum was finally demonstrated by silencing the Pcku70 gene to increase homologous recombination frequency. This led to the generation of single and double knockout mutants...
  10. pmc Three alpha-subunits of heterotrimeric G proteins and an adenylyl cyclase have distinct roles in fruiting body development in the homothallic fungus Sordaria macrospora
    Jens Kamerewerd
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    Genetics 180:191-206. 2008
    ..From the sum of all our data, we propose a model for how different Galpha-subunits interact with pheromone receptors, adenylyl cyclase, and STE12 and thus cooperatively regulate sexual development in S. macrospora...
  11. ncbi request reprint Highly efficient generation of signal transduction knockout mutants using a fungal strain deficient in the mammalian ku70 ortholog
    Stefanie Pöggeler
    Ruhr Universitat Bochum, Lehrstuhl für Allgemeine und Molekulare Botanik, 44780 Bochum, Germany
    Gene 378:1-10. 2006
    ..PCR generated deletion constructs containing 1000 bp of homologous flanking sequence resulted in a drastically increased gene targeting efficiency. As a consequence, almost all transformants generated carried a disrupted target gene...
  12. ncbi request reprint Identification of a minimal cre1 promoter sequence promoting glucose-dependent gene expression in the beta-lactam producer Acremonium chrysogenum
    Danielle Janus
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat, Universitatsstr 150, 44780 Bochum, Germany
    Curr Genet 53:35-48. 2008
    ..Our results will contribute to the construction of versatile expression vectors carrying a minimal cre1 promoter sequence that still confers glucose-dependent induction of gene expression...
  13. pmc Application of the Saccharomyces cerevisiae FLP/FRT recombination system in filamentous fungi for marker recycling and construction of knockout strains devoid of heterologous genes
    Katarina Kopke
    Ruhr Universitat Bochum, Universitatsstrasse 150, 44780 Bochum, Germany
    Appl Environ Microbiol 76:4664-74. 2010
    ..Here, we discuss the application of the optimized FLP/FRT recombination system as a molecular tool for the genetic manipulation of filamentous fungi...
  14. pmc The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora
    Minou Nowrousian
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    Mol Microbiol 64:923-37. 2007
    ..Taken together, these data show that PRO41 is a novel ER membrane protein essential for fruiting body formation in filamentous fungi...
  15. pmc The WW domain protein PRO40 is required for fungal fertility and associates with Woronin bodies
    Ines Engh
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, ND7 131, Universitatsstrasse 150, 44780 Bochum, Germany
    Eukaryot Cell 6:831-43. 2007
    ..macrospora and Neurospora crassa, as shown by fluorescence microscopy, sedimentation, and immunoblot analyses. We discuss the function of PRO40 in fruiting body formation...
  16. pmc A homologue of the Aspergillus velvet gene regulates both cephalosporin C biosynthesis and hyphal fragmentation in Acremonium chrysogenum
    Jacqueline Dreyer
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat, Universitatsstr 150, D 44780 Bochum, Germany
    Appl Environ Microbiol 73:3412-22. 2007
    ..Our findings will be significant for biotechnical processes that require a defined stage of cellular differentiation for optimal production of secondary metabolites...
  17. ncbi request reprint Regulation of melanin biosynthesis via the dihydroxynaphthalene pathway is dependent on sexual development in the ascomycete Sordaria macrospora
    Ines Engh
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    FEMS Microbiol Lett 275:62-70. 2007
    ....
  18. ncbi request reprint Multiple layers of temporal and spatial control regulate accumulation of the fruiting body-specific protein APP in Sordaria macrospora and Neurospora crassa
    Minou Nowrousian
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universität Bochum ND 7 130, Universitatsstr 150, 44780 Bochum, Germany
    Fungal Genet Biol 44:602-14. 2007
    ..These findings highlight multiple layers of temporal and spatial control of gene expression during fungal development...
  19. doi request reprint A homologue of the human STRIPAK complex controls sexual development in fungi
    Sandra Bloemendal
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Universitatsstr 150, 44780 Bochum, Germany
    Mol Microbiol 84:310-23. 2012
    ..In S. macrospora, truncation of PRO11 and PRO22 leads to distinct defects in sexual development and cell fusion, indicating a role for the fungal STRIPAK complex in both processes...
  20. doi request reprint PcchiB1, encoding a class V chitinase, is affected by PcVelA and PcLaeA, and is responsible for cell wall integrity in Penicillium chrysogenum
    Jens Kamerewerd
    Christian Doppler Laboratory for Fungal Biotechnology, Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Universitatsstrase 150, 44780 Bochum, Germany
    Microbiology 157:3036-48. 2011
    ..We discuss the relevance of this class V chitinase for morphology in an industrially important fungus...
  21. pmc De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis
    Minou Nowrousian
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    PLoS Genet 6:e1000891. 2010
    ....
  22. doi request reprint Homologous recombination in the antibiotic producer Penicillium chrysogenum: strain DeltaPcku70 shows up-regulation of genes from the HOG pathway
    Birgit Hoff
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    Appl Microbiol Biotechnol 85:1081-94. 2010
    ..Furthermore, we discuss strategies to reactivate Pcku70 in strains successfully used for gene disruptions...
  23. ncbi request reprint Detection of hyphal fusion in filamentous fungi using differently fluorescence-labeled histones
    Christine Rech
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, ND7 131, Universitatsstrasse 150, 44780 Bochum, Germany
    Curr Genet 52:259-66. 2007
    ..The simple and efficient HAMF assay described here could detect even rare fusion events and should be applicable to a broad range of diverse fungal species including those lacking male or female reproductive structures or asexual spores...
  24. pmc Winged helix transcription factor CPCR1 is involved in regulation of beta-lactam biosynthesis in the fungus Acremonium chrysogenum
    Esther K Schmitt
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, D 44780 Bochum, Germany
    Eukaryot Cell 3:121-34. 2004
    ..However, the complexity of the data points to a well-controlled or even functional redundant network of transcription factors, with CPCR1 being only one player within this process...
  25. pmc Eighty years after its discovery, Fleming's Penicillium strain discloses the secret of its sex
    Birgit Hoff
    Ruhr Universitat Bochum, Lehrstuhl für Allgemeine und Molekulare Botanik, Universitatsstr 150, 44780 Bochum, Germany
    Eukaryot Cell 7:465-70. 2008
    ..This discovery extends options for industrial strain improvement programs using conventional genetical approaches...
  26. doi request reprint Sordaria macrospora, a model organism to study fungal cellular development
    Ines Engh
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, 44780 Bochum, Germany
    Eur J Cell Biol 89:864-72. 2010
    ..This review summarizes the results of our recent findings, thus advancing current knowledge of the general principles and paradigms underpinning eukaryotic cell differentiation and development...
  27. ncbi request reprint Comparative gene expression analysis of fruiting body development in two filamentous fungi
    Minou Nowrousian
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    FEMS Microbiol Lett 257:328-35. 2006
    ..The analyses revealed similar expression patterns during sexual development for several of the S. macrospora genes when compared with P. confluens...
  28. pmc An efficient fungal RNA-silencing system using the DsRed reporter gene
    Danielle Janus
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat, Universitatsstr 150, D 44780 Bochum, Germany
    Appl Environ Microbiol 73:962-70. 2007
    ..The RNA-silencing system presented here should be a powerful genetic tool for strain improvement and genome-wide analysis of this biotechnologically important filamentous fungus...
  29. ncbi request reprint CPCR1, but not its interacting transcription factor AcFKH1, controls fungal arthrospore formation in Acremonium chrysogenum
    Birgit Hoff
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat, Universitatsstrasse 150, D 44780 Bochum, Germany
    Mol Microbiol 56:1220-33. 2005
    ..chrysogenum. Our data offer the potential to control fungal growth in biotechnical processes that require defined morphological stages for optimal production yields...
  30. doi request reprint Cell-to-cell communication in plants, animals, and fungi: a comparative review
    Sandra Bloemendal
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, ND7 131, Universitatsstrase 150, Bochum, 44780, Germany
    Naturwissenschaften 100:3-19. 2013
    ..This review summarizes the underlying mechanisms for intercellular communication in these three eukaryotic groups and discusses its consequences for the regulation of differentiation and developmental processes...
  31. pmc Whole-Genome Sequencing of Sordaria macrospora Mutants Identifies Developmental Genes
    Minou Nowrousian
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, 44780 Bochum, Germany
    G3 (Bethesda) 2:261-70. 2012
    ....
  32. ncbi request reprint Ds-like restless deletion derivatives occur in Tolypocladium inflatum and two foreign hosts, Neurospora crassa and Penicillium chrysogenum
    Frank Windhofer
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, Bochum, Germany
    Fungal Genet Biol 35:171-82. 2002
    ..In addition Ds1-like Restless elements were identified that carried foreign sequences between the inverted repeats. We discuss how Ds-like Restless elements might be generated by inaccurate excision from an active transposon copy...
  33. ncbi request reprint AcFKH1, a novel member of the forkhead family, associates with the RFX transcription factor CPCR1 in the cephalosporin C-producing fungus Acremonium chrysogenum
    Esther K Schmitt
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, D 44801 Bochum, Germany
    Gene 342:269-81. 2004
    ..2 kb promoter region of the divergently oriented cephalosporin biosynthesis gene pair pcbAB-pcbC from A. chrysogenum. Additionally, AcFKH1 is able to bind with high affinity to the SWI5-binding site of the yeast FKH2 protein...
  34. pmc Identification of a chloroplast ribonucleoprotein complex containing trans-splicing factors, intron RNA, and novel components
    Jessica Jacobs
    Department for General and Molecular Botany, Ruhr University Bochum, Bochum, Germany
    Mol Cell Proteomics 12:1912-25. 2013
    ..Our findings contribute to our knowledge of chloroplast multisubunit ribonucleoprotein complexes and are discussed in support of the generally accepted view that group II introns are the ancestors of the eukaryotic spliceosomal introns. ..
  35. doi request reprint Function of chloroplast RNA-binding proteins
    Jessica Jacobs
    Department for General and Molecular Biology, Ruhr University Bochum, Universitatsstrase 150, Bochum, Germany
    Cell Mol Life Sci 68:735-48. 2011
    ..Such data will greatly contribute to a better understanding of the biogenesis of a unique organelle found in all photosynthetic organisms...
  36. ncbi request reprint A Sordaria macrospora mutant lacking the leu1 gene shows a developmental arrest during fruiting body formation
    Ulrich Kück
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat, Bochum, Germany
    Mol Genet Genomics 274:307-15. 2005
    ..Taken together, these data show that pro4 represents a novel mutant type in S. macrospora, in which amino acid starvation acts as a signal that interrupts the development of the fruiting body...
  37. ncbi request reprint Cross-species microarray hybridization to identify developmentally regulated genes in the filamentous fungus Sordaria macrospora
    Minou Nowrousian
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universität Bochum ND 7 131, Universitatsstr 150, 44801, Bochum, Germany
    Mol Genet Genomics 273:137-49. 2005
    ..Analysis of gene expression in double mutants revealed a complex network of interaction between the pro gene products...
  38. ncbi request reprint Functional analysis of the C6 zinc finger gene pro1 involved in fungal sexual development
    Sandra Masloff
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, D 44780 Bochum, Germany
    Fungal Genet Biol 36:107-16. 2002
    ..crassa genome, we identified hpro1A, another transcriptionally expressed gene, with a similarity of 40% to the pro1 genes, which is present as a single copy gene in N. crassa as well as in S. macrospora...
  39. ncbi request reprint DNA macroarray and real-time PCR analysis of two nuclear photosystem I mutants from Chlamydomonas reinhardtii reveal downregulation of Lhcb genes but different regulation of Lhca genes
    Carsten Balczun
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, D 44780 Bochum, Germany
    Biochim Biophys Acta 1732:62-8. 2005
    ..Taken together, our data suggest that a signal is transmitted from the chloroplast to the nucleus that serves to regulate the level of light harvesting polypeptides in the organelle...
  40. pmc A nucleosome assembly protein-like polypeptide binds to chloroplast group II intron RNA in Chlamydomonas reinhardtii
    Stephanie Glanz
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat Bochum, D 44780 Bochum, Germany
    Nucleic Acids Res 34:5337-51. 2006
    ..These data indicate that the chloroplast protein described here is a novel member of the NAP family and most probably has not been acquired from a prokaryotic endosymbiont...
  41. ncbi request reprint Use of bimolecular fluorescence complementation to demonstrate transcription factor interaction in nuclei of living cells from the filamentous fungus Acremonium chrysogenum
    Birgit Hoff
    Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr Universitat, 44801 Bochum, Germany
    Curr Genet 47:132-8. 2005
    ..The nuclear localization of the protein-protein interaction was verified by staining fungal cells with the nucleic acid dye TOTO-3...