S Jakobs

Summary

Country: Germany

Publications

  1. ncbi request reprint High resolution imaging of live mitochondria
    Stefan Jakobs
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Mitochondrial Structure and Dynamics Group, Am Fassberg 11, 37077 Goettingen, Germany
    Biochim Biophys Acta 1763:561-75. 2006
  2. pmc The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates
    Stefan Stoldt
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Biol Cell 23:2292-301. 2012
  3. pmc Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms
    Daniel Neumann
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    PMC Biophys 3:4. 2010
  4. ncbi request reprint Light microscopic analysis of mitochondrial heterogeneity in cell populations and within single cells
    Stefan Jakobs
    Mitochondrial Structure and Dynamics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Goettingen, Germany
    Adv Biochem Eng Biotechnol 124:1-19. 2011
  5. ncbi request reprint EFGP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy
    S Jakobs
    Max Planck Institute for Biophysicial Chemistry, High Resolution Optical Microscopy Group, Gottingen, Germany
    FEBS Lett 479:131-5. 2000
  6. ncbi request reprint Photoconversion of matrix targeted GFP enables analysis of continuity and intermixing of the mitochondrial lumen
    Stefan Jakobs
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    FEBS Lett 554:194-200. 2003
  7. doi request reprint Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 26:1035-40. 2008
  8. doi request reprint Rapid FlAsH labelling in the budding yeast Saccharomyces cerevisiae
    C A Wurm
    Mitochondrial Structure and Dynamics Group, Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Microsc 240:6-13. 2010
  9. ncbi request reprint Spatial and temporal dynamics of budding yeast mitochondria lacking the division component Fis1p
    Stefan Jakobs
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Cell Sci 116:2005-14. 2003
  10. pmc Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters
    Alexander Egner
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 93:3285-90. 2007

Collaborators

  • Christian Eggeling
  • Alexander Egner
  • S W Hell
  • Astrid C Schauss
  • Johannes M Herrmann
  • B Westermann
  • Gerrit Groenhof
  • R Jahn
  • Christian A Wurm
  • Martin Andresen
  • Andre C Stiel
  • Rebecca Medda
  • Roman Schmidt
  • Birka Hein
  • Andreas Schönle
  • Gert Weber
  • Simon Trowitzsch
  • Markus C Wahl
  • Dirk Wenzel
  • Daniel Neumann
  • Jonas Fölling
  • Hannes Bock
  • Katrin I Willig
  • Katrin Altmann
  • Jörg Bewersdorf
  • Michael Hofmann
  • Stefan Stoldt
  • Kai Stefan Dimmer
  • C A Wurm
  • Tanja Brakemann
  • Ida E Suppanz
  • Volker Westphal
  • Ilaria Testa
  • Mariano Bossi
  • Helmut Grubmuller
  • Miriam A Schwentker
  • Gerald Donnert
  • Johann Engelhardt
  • Hilmar Gugel
  • Frank Vogel
  • Marcus Dyba
  • Marlies Messerschmitt
  • K Bahlmann
  • Markus Hildenbeutel
  • H Kano
  • I E Suppanz
  • S Stoldt
  • Lars Kastrup
  • Johanna Bückers
  • Annedore Punge
  • Jessica Schilde
  • Claudia Geisler
  • Martina Frank
  • Claas von Middendorff
  • Michael Hilbert
  • Silvio O Rizzoli
  • Jan Keller
  • Robert R Kellner
  • Frauke Gräter
  • Lars V Schäfer
  • Rafael Storz
  • Rita Schmitz-Salue
  • Stefan Fritz
  • Walter Neupert
  • M Nagorni

Detail Information

Publications39

  1. ncbi request reprint High resolution imaging of live mitochondria
    Stefan Jakobs
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Mitochondrial Structure and Dynamics Group, Am Fassberg 11, 37077 Goettingen, Germany
    Biochim Biophys Acta 1763:561-75. 2006
    ..In particular, approaches to study the movement of mitochondrial proteins and novel imaging techniques (FRET imaging-, 4Pi- and STED-microscopy) that provide high spatial resolution are considered...
  2. pmc The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates
    Stefan Stoldt
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Biol Cell 23:2292-301. 2012
    ..Our findings show that the distribution of Oxa1 within the inner membrane is dynamic and adapts to different physiological needs...
  3. pmc Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms
    Daniel Neumann
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    PMC Biophys 3:4. 2010
    ..pacs: 87.16.tb, 87.85.rs...
  4. ncbi request reprint Light microscopic analysis of mitochondrial heterogeneity in cell populations and within single cells
    Stefan Jakobs
    Mitochondrial Structure and Dynamics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Goettingen, Germany
    Adv Biochem Eng Biotechnol 124:1-19. 2011
    ..Therefore, we briefly discuss here the potential of subdiffraction light microscopy (nanoscopy) to study inner-mitochondrial heterogeneities...
  5. ncbi request reprint EFGP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy
    S Jakobs
    Max Planck Institute for Biophysicial Chemistry, High Resolution Optical Microscopy Group, Gottingen, Germany
    FEBS Lett 479:131-5. 2000
    ..In aging bacterial cultures DsRed appeared to aggregate within the cells, accompanied by a strong reduction in its fluorescence lifetime as determined by fluorescence lifetime imaging microscopy...
  6. ncbi request reprint Photoconversion of matrix targeted GFP enables analysis of continuity and intermixing of the mitochondrial lumen
    Stefan Jakobs
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    FEBS Lett 554:194-200. 2003
    ..Matrix constrictions frequently occurring in wild type cells as well as in Deltafis1 and Deltadnm1 mutants do not interfere with luminal continuity...
  7. doi request reprint Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 26:1035-40. 2008
    ..Furthermore, we demonstrate dual-color fluorescence microscopy with sub-diffraction resolution using bsDronpa and Dronpa whose emission maxima are separated by <20 nm...
  8. doi request reprint Rapid FlAsH labelling in the budding yeast Saccharomyces cerevisiae
    C A Wurm
    Mitochondrial Structure and Dynamics Group, Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Microsc 240:6-13. 2010
    ..This approach may be used for cells in different growth phases or grown under different conditions. It may be further extended to other small high affinity probes, thus opening up new possibilities for labelling in budding yeast...
  9. ncbi request reprint Spatial and temporal dynamics of budding yeast mitochondria lacking the division component Fis1p
    Stefan Jakobs
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Cell Sci 116:2005-14. 2003
    ..The data suggest that different molecular machineries are responsible for the separation of the matrix and the fission of the outer membrane in budding yeast...
  10. pmc Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters
    Alexander Egner
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 93:3285-90. 2007
    ..These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm...
  11. ncbi request reprint Dual-color 4Pi-confocal microscopy with 3D-resolution in the 100 nm range
    H Kano
    High Resolution Optical Microscopy Group, Max-Planck Institute for Biophysical Chemistry, , Germany
    Ultramicroscopy 90:207-13. 2001
    ..The synergistic combination of dual-color 4Pi-confocal recording with image restoration results in dual-color imaging with a 3D resolution in the 100 nm range...
  12. ncbi request reprint 4Pi-confocal microscopy of live cells
    K Bahlmann
    High Resolution Optical Microscopy Group, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Ultramicroscopy 87:155-64. 2001
    ..The first images of live biological samples with an all-directional resolution in the 190-280 nm range are presented here, thus establishing a new resolution benchmark in live-cell microscopy...
  13. ncbi request reprint Reversible photoswitching enables single-molecule fluorescence fluctuation spectroscopy at high molecular concentration
    C Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Microsc Res Tech 70:1003-9. 2007
    ..Photoswitching expands the range of single-molecule detection based experiments such as fluorescence fluctuation spectroscopy to large entity concentrations in the micromolar range...
  14. pmc Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy
    Andre C Stiel
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 95:2989-97. 2008
    ..We demonstrate time-lapse live-cell subdiffraction microscopy by imaging rsCherryRev targeted to the endoplasmic reticulum utilizing the switching and localization of single molecules...
  15. pmc The m-AAA protease processes cytochrome c peroxidase preferentially at the inner boundary membrane of mitochondria
    Ida E Suppanz
    Department of NanoBiophotonics Mitochondrial Structure and Dynamics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Biol Cell 20:572-80. 2009
    ..These findings suggest that protein quality control and proteolytic activation exerted by the m-AAA protease take place preferentially in the IBM pointing to significant functional differences between the IBM and the CM...
  16. ncbi request reprint Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching
    Miriam A Schwentker
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Microsc Res Tech 70:269-80. 2007
    ..The obtained resolution of 50 nm ( approximately lambda/12) is limited only by the spectroscopic properties of the proteins and the imperfections of the optical implementation, but not on principle grounds...
  17. doi request reprint Spherical nanosized focal spot unravels the interior of cells
    Roman Schmidt
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 5:539-44. 2008
    ..Fully relying on focused light, this lens-based fluorescence nanoscope unravels the interior of cells noninvasively, uniquely dissecting their sub-lambda-sized organelles...
  18. doi request reprint Mitochondrial cristae revealed with focused light
    Roman Schmidt
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37077 Gottingen, Germany
    Nano Lett 9:2508-10. 2009
    ..We find a pronounced heterogeneity in the cristae arrangements even within individual mitochondrial tubules...
  19. ncbi request reprint Nanoscale resolution in GFP-based microscopy
    Katrin I Willig
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 3:721-3. 2006
    ..Our results mark the advent of nanoscale biological microscopy with genetically encoded markers...
  20. ncbi request reprint Fis1p and Caf4p, but not Mdv1p, determine the polar localization of Dnm1p clusters on the mitochondrial surface
    Astrid C Schauss
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    J Cell Sci 119:3098-106. 2006
    ..This study suggests that Caf4p plays a key role in determining the polarized localization of those Dnm1p clusters that are not immediately involved in the mitochondrial fission process...
  21. pmc Stimulated emission depletion nanoscopy of living cells using SNAP-tag fusion proteins
    Birka Hein
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
    Biophys J 98:158-63. 2010
    ..Hence fusion proteins that bind modified organic dyes expand widely the application range of far-field fluorescence nanoscopy of living cells...
  22. pmc Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins
    Michael Hofmann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 102:17565-9. 2005
    ..Our results underscore the potential to finally achieve molecular resolution in fluorescence microscopy by technical optimization...
  23. pmc Short tetracysteine tags to beta-tubulin demonstrate the significance of small labels for live cell imaging
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Biol Cell 15:5616-22. 2004
    ..The presented approach to tag open reading frames (ORFs) at their native loci with very small TetCys-tags and the subsequent visualization of the tagged proteins in vivo can be extended in principle to any ORF in S. cerevisiae...
  24. ncbi request reprint Nanoscale separation of molecular species based on their rotational mobility
    Ilaria Testa
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 16:21093-104. 2008
    ..Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells...
  25. pmc Fast 100-nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast
    Alexander Egner
    High Resolution Optical Microscopy Group, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 99:3370-5. 2002
    ..Moreover, this change is associated with a 2.8-fold increase of the surface of the reticulum, resulting in an average increase in volume of the mitochondrial compartment by a factor of 3.0 +/- 0.2...
  26. pmc Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron
    Tanja Brakemann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Biol Chem 285:14603-9. 2010
    ..Distinct absorption cross-sections for the switching wavelengths in the fluorescent and the nonfluorescent state are not essential for efficient photochromism in fluorescent proteins, although they may facilitate the switching process...
  27. pmc 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants
    Andre C Stiel
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Biochem J 402:35-42. 2007
    ..The findings reported in the present study support the view that a cis-trans isomerization is one of the key events common to the switching mechanism in RSFPs...
  28. pmc Structural basis for reversible photoswitching in Dronpa
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 104:13005-9. 2007
    ..We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore...
  29. ncbi request reprint Concepts for nanoscale resolution in fluorescence microscopy
    Stefan W Hell
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37070 Gottingen, Germany
    Curr Opin Neurobiol 14:599-609. 2004
    ..Formed on the basis of reversible saturable optical transitions, these concepts might eventually allow us to investigate hitherto inaccessible details within live cells...
  30. ncbi request reprint Differential protein distributions define two sub-compartments of the mitochondrial inner membrane in yeast
    Christian A Wurm
    Mitochondrial Structure and Dynamics Group, Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Gottingen, Germany
    FEBS Lett 580:5628-34. 2006
    ..The different protein distributions shown here demonstrate that the cristae membranes and the IBM are functionally distinct sub-compartments...
  31. pmc Structure and mechanism of the reversible photoswitch of a fluorescent protein
    Martin Andresen
    Department of NanoBiophotonics, Theoretical and Computational Biophysics, and X Ray Crystallography, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 102:13070-4. 2005
    ..Reversible photoswitching of the protein chromophore system within intact crystals also constitutes a step toward the use of fluorescent proteins in three-dimensional data recording...
  32. ncbi request reprint Immunofluorescence stimulated emission depletion microscopy
    Marcus Dyba
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Nat Biotechnol 21:1303-4. 2003
    ..We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation...
  33. doi request reprint Fluorescence nanoscopy by ground-state depletion and single-molecule return
    Jonas Fölling
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 5:943-5. 2008
    ..Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach...
  34. ncbi request reprint 4Pi microscopy of quantum dot-labeled cellular structures
    Rebecca Medda
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37070 Gottingen, Germany
    J Struct Biol 156:517-23. 2006
    ..In particular, we visualize the three-dimensional entanglement of the two networks with unprecedented detail...
  35. pmc Mdm31 and Mdm32 are inner membrane proteins required for maintenance of mitochondrial shape and stability of mitochondrial DNA nucleoids in yeast
    Kai Stefan Dimmer
    Institut fur Physiologische Chemie, Universitat Munchen, 81377 Munchen, Germany
    J Cell Biol 168:103-15. 2005
    ..We propose that Mdm31 and Mdm32 cooperate with Mmm1, Mmm2, Mdm10, and Mdm12 in maintenance of mitochondrial morphology and mtDNA...
  36. pmc The inner membrane protein Mdm33 controls mitochondrial morphology in yeast
    Marlies Messerschmitt
    Institut fur Physiologische Chemie, Universitat Munchen, D 81377 Munchen, Germany
    J Cell Biol 160:553-64. 2003
    ..Our results indicate that Mdm33 plays a distinct role in the mitochondrial inner membrane to control mitochondrial morphology. We propose that Mdm33 is involved in fission of the mitochondrial inner membrane...
  37. pmc The class V myosin motor protein, Myo2, plays a major role in mitochondrial motility in Saccharomyces cerevisiae
    Katrin Altmann
    Institut fur Zellbiologie, Universitat Bayreuth, 95440 Bayreuth, Germany
    J Cell Biol 181:119-30. 2008
    ..We conclude that Myo2 plays an important and direct role for mitochondrial motility and inheritance in budding yeast...
  38. pmc Two-color far-field fluorescence nanoscopy
    Gerald Donnert
    Biophys J 92:L67-9. 2007
    ..The joint improvement of resolution and colocalization demonstrates the emerging potential of far-field fluorescence nanoscopy to study the spatial organization of macromolecules in cells...
  39. pmc Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy
    Hilmar Gugel
    Leica Microsystems Heidelberg GmbH, 68165 Mannheim, Germany
    Biophys J 87:4146-52. 2004
    ..Realized in a state-of-the-art scanning microscope, this approach enables robust three-dimensional imaging of fixed and live cells at approximately 80 nm axial resolution...