R Jahn

Summary

Country: Germany

Publications

  1. ncbi request reprint Neuroscience. A neuronal receptor for botulinum toxin
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Science 312:540-1. 2006
  2. doi request reprint Molecular machines governing exocytosis of synaptic vesicles
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nature 490:201-7. 2012
  3. ncbi request reprint Synaptic transmission: two players team up for a new tune
    R Jahn
    Department of Neurobiology Max Planck Institute for Biophysical Chemistry Am Fassberg D 37077 Göttingen, Germany
    Curr Biol 8:R856-8. 1998
  4. doi request reprint Some classic papers in the field of membrane fusion--a personal view
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nat Struct Mol Biol 15:655-7. 2008
  5. ncbi request reprint SNAREs--engines for membrane fusion
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 37077 Gottingen, Germany
    Nat Rev Mol Cell Biol 7:631-43. 2006
  6. ncbi request reprint Principles of exocytosis and membrane fusion
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Ann N Y Acad Sci 1014:170-8. 2004
  7. ncbi request reprint Membrane fusion and exocytosis
    R Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Annu Rev Biochem 68:863-911. 1999
  8. ncbi request reprint Membrane fusion
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 37077, Gottingen, Germany
    Curr Opin Cell Biol 14:488-95. 2002
  9. ncbi request reprint Membrane fusion
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Cell 112:519-33. 2003
  10. ncbi request reprint Reconstitution of regulated exocytosis in cell-free systems: a critical appraisal
    J Avery
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Annu Rev Physiol 61:777-807. 1999

Collaborators

Detail Information

Publications92

  1. ncbi request reprint Neuroscience. A neuronal receptor for botulinum toxin
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Science 312:540-1. 2006
  2. doi request reprint Molecular machines governing exocytosis of synaptic vesicles
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nature 490:201-7. 2012
    ..Using mainly a biochemical and biophysical perspective, we briefly survey the molecular mechanisms in an attempt to functionally integrate the key proteins into the emerging picture of the neuronal fusion machine...
  3. ncbi request reprint Synaptic transmission: two players team up for a new tune
    R Jahn
    Department of Neurobiology Max Planck Institute for Biophysical Chemistry Am Fassberg D 37077 Göttingen, Germany
    Curr Biol 8:R856-8. 1998
    ..The AMPA-subclass glutamate receptor GluR2 interacts with the membrane-fusion protein NSF in a manner that appears important for receptor-mediated intracellular signalling...
  4. doi request reprint Some classic papers in the field of membrane fusion--a personal view
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nat Struct Mol Biol 15:655-7. 2008
  5. ncbi request reprint SNAREs--engines for membrane fusion
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 37077 Gottingen, Germany
    Nat Rev Mol Cell Biol 7:631-43. 2006
    ..A fascinating picture of these robust nanomachines is emerging...
  6. ncbi request reprint Principles of exocytosis and membrane fusion
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Ann N Y Acad Sci 1014:170-8. 2004
    ..In contrast, less is known about regulatory mechanisms. Here, a brief overview is given about the current state of knowledge, focusing on SNAREs involved in neuronal exocytosis...
  7. ncbi request reprint Membrane fusion and exocytosis
    R Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Annu Rev Biochem 68:863-911. 1999
    ..Recent evidence suggests that Rab proteins function in the initial membrane contact connecting the fusing membranes but are not involved in the fusion reaction itself...
  8. ncbi request reprint Membrane fusion
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 37077, Gottingen, Germany
    Curr Opin Cell Biol 14:488-95. 2002
    ..Despite substantial progress, an integrated concept for protein-mediated membrane fusion is not yet available, and many open questions remain to be answered...
  9. ncbi request reprint Membrane fusion
    Reinhard Jahn
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Cell 112:519-33. 2003
    ..Cellular fusion machines are adapted to fit the needs of different reactions but operate by similar principles in order to achieve merging of the bilayers...
  10. ncbi request reprint Reconstitution of regulated exocytosis in cell-free systems: a critical appraisal
    J Avery
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Annu Rev Physiol 61:777-807. 1999
    ..In this chapter, we review some of the literature in the light of these new developments and attempt to provide a critical discussion of the strengths and limitations of the various cell-free systems...
  11. pmc A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function
    W Antonin
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    EMBO J 19:6453-64. 2000
    ..We conclude that the structure of core complexes and their molecular mechanism in membrane fusion is highly conserved between distant SNAREs...
  12. ncbi request reprint Homo- and heterooligomeric SNARE complexes studied by site-directed spin labeling
    M Margittai
    Department of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, , Germany
    J Biol Chem 276:13169-77. 2001
    ..The binary complex of syntaxin and SNAP-25 consists of a parallel four helix-bundle with properties resembling that of the ternary complex...
  13. ncbi request reprint Identification of differentiation-associated brain-specific phosphate transporter as a second vesicular glutamate transporter (VGLUT2)
    S Takamori
    Departments of Neurobiology and Membrane Biophysics, Max-Planck-Institute for Biophysical Chemistry, , Germany
    J Neurosci 21:RC182. 2001
    ..Thus, expression of VGLUT2 suffices to cause GABAergic neurons to release glutamate in addition to GABA in a manner very similar to that reported previously for VGLUT1...
  14. ncbi request reprint Selective interaction of complexin with the neuronal SNARE complex. Determination of the binding regions
    S Pabst
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    J Biol Chem 275:19808-18. 2000
    ..Complexins may regulate the function of ternary complexes and control membrane fusion through this interaction...
  15. pmc SNAREs are concentrated in cholesterol-dependent clusters that define docking and fusion sites for exocytosis
    T Lang
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    EMBO J 20:2202-13. 2001
    ..This suggests that high local concentrations of SNAREs are required for efficient fusion...
  16. ncbi request reprint Rapid and selective binding to the synaptic SNARE complex suggests a modulatory role of complexins in neuroexocytosis
    Stefan Pabst
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen D 37077, Germany
    J Biol Chem 277:7838-48. 2002
    ..Furthermore, complexin did not promote the formation of SNARE complex oligomers. Together, our data suggest that complexins modulate neuroexocytosis after assembly of membrane-bridging SNARE complexes...
  17. ncbi request reprint Reinvestigation of the role of snapin in neurotransmitter release
    Olga Vites
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Biol Chem 279:26251-6. 2004
    ..We conclude that the postulated role of snapin as a SNARE regulator in neurotransmitter release needs reconsideration, leaving the true function of this evolutionarily conserved protein to be discovered...
  18. ncbi request reprint Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties
    D Fasshauer
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    J Biol Chem 274:15440-6. 1999
    ....
  19. pmc The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes
    W Antonin
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Mol Biol Cell 11:3289-98. 2000
    ..Recombinant endobrevin/VAMP-8 inhibited both fusion reactions with similar potency. We conclude that endobrevin/VAMP-8 operates as an R-SNARE in the homotypic fusion of early and late endosomes...
  20. pmc Single-molecule fluorescence resonance energy transfer reveals a dynamic equilibrium between closed and open conformations of syntaxin 1
    M Margittai
    Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 100:15516-21. 2003
    ..We found that free syntaxin switches between an inactive closed and an active open configuration with a relaxation time of 0.8 ms, explaining why regulatory proteins are needed to arrest the protein in one conformational state...
  21. ncbi request reprint Quantal release of serotonin
    D Bruns
    Max Planck Institute for Biophysical Chemistry, Department of Neurobiology, Gottingen, Germany
    Neuron 28:205-20. 2000
    ..On average, differences in the transmitter amount released from SSVs and LDCVs are proportional to the size differences of the organelles, suggesting that transmitter is stored at similar concentrations in SSVs and LDCVs...
  22. ncbi request reprint The N-terminal domains of syntaxin 7 and vti1b form three-helix bundles that differ in their ability to regulate SNARE complex assembly
    Wolfram Antonin
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Biol Chem 277:36449-56. 2002
    ..However, they differ in their ability to adopt closed conformations and thus to regulate the assembly of SNARE complexes...
  23. ncbi request reprint Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons
    S Takamori
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Nature 407:189-94. 2000
    ..We conclude that BNPI functions as vesicular glutamate transporter and that expression of BNPI suffices to define a glutamatergic phenotype in neurons...
  24. pmc Exocytosis requires asymmetry in the central layer of the SNARE complex
    R Ossig
    Department of Neurobiology, Department of Molecular Genetics and Theoretical Molecular Biophysics Group, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    EMBO J 19:6000-10. 2000
    ..We conclude that the asymmetric arrangement of the polar amino acids in the central layer is essential for normal function of SNAREs in membrane fusion...
  25. ncbi request reprint Neuroscience. Synaptic vesicles in the fast lane
    Matthew Holt
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Science 303:1986-7. 2004
  26. ncbi request reprint A dual function for Munc-18 in exocytosis of PC12 cells
    Dagmar Schütz
    Max Planck Institute for Biophysical Chemistry, Department of Neurobiology, Am Fassberg 11, 37077 Gottingen, Germany
    Eur J Neurosci 21:2419-32. 2005
    ..We propose that Munc-18 represents a dynamic link between syntaxin-related and Mint1-related mechanisms, both involved in the control of LDCV exocytosis in neuroendocrine cells...
  27. pmc A novel site of action for alpha-SNAP in the SNARE conformational cycle controlling membrane fusion
    Marcin Barszczewski
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Biol Cell 19:776-84. 2008
    ..We conclude that alpha-SNAP inhibits exocytosis by binding to the syntaxin SNARE motif and in turn prevents SNARE assembly, revealing an unexpected site of action for alpha-SNAP in the SNARE cycle that drives exocytotic membrane fusion...
  28. pmc Determinants of liposome fusion mediated by synaptic SNARE proteins
    Christina G Schuette
    Max Planck Institute for Biophysical Chemistry, 37077 Goettingen, Germany
    Proc Natl Acad Sci U S A 101:2858-63. 2004
    ..We conclude that the rate of liposome fusion is dominated by the intrinsic properties of the SNAREs rather than by the preceding docking step...
  29. pmc Determinants of synaptobrevin regulation in membranes
    Tabrez J Siddiqui
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Biol Cell 18:2037-46. 2007
    ....
  30. doi request reprint Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses
    Henrik Martens
    Synaptic Systems GmbH, Gottingen, Germany
    J Neurosci 28:13125-31. 2008
    ..Overall, our data open the possibility of studying novel GABA release sites, characterizing inhibitory vesicle trafficking, and establishing their contribution to inhibitory neurotransmission at identified GABAergic synapses...
  31. ncbi request reprint The R-SNARE motif of tomosyn forms SNARE core complexes with syntaxin 1 and SNAP-25 and down-regulates exocytosis
    Kiyotaka Hatsuzawa
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, D 37077 Gottingen, Germany
    J Biol Chem 278:31159-66. 2003
    ..We conclude that tomosyn is a soluble SNARE that directly competes with synaptobrevin in the formation of SNARE complexes and thus may function in down-regulating exocytosis...
  32. ncbi request reprint Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs
    Wolfram Antonin
    Department of Neurobiology and Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Struct Biol 9:107-11. 2002
    ..We conclude that the structure of the SNARE core complex is an evolutionarily conserved hallmark of all SNARE complexes and is intimately associated with the general role of SNAREs in membrane fusion...
  33. ncbi request reprint Live cell imaging by multifocal multiphoton microscopy
    M Straub
    High Resolution Optical Microscopy Group, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Eur J Cell Biol 79:726-34. 2000
    ..We conclude that MMM proves to be a technically simple and very effective method for fast 3-D live cell imaging at high resolution...
  34. doi request reprint Synaptic vesicles studied by dynamic light scattering
    S Castorph
    Institut fur Rontgenphysik, Georg August Universitat Gottingen, Germany
    Eur Phys J E Soft Matter 34:1-11. 2011
    ..The size increase of the proteoliposomes due to SNARE-dependent fusion with SVs is quantified by DLS under quasi-physiological conditions...
  35. ncbi request reprint Molecular determinants of exocytosis
    Dieter Bruns
    Max Planck Institute for Biophysical Chemistry, Department of Neurobiology, Am Fassberg 11, 37077 Gottingen, Germany
    Pflugers Arch 443:333-8. 2002
    ..Structural and functional studies have provided a conceptual framework and a starting point for our mechanistic understanding of how SNARE proteins may contribute to the final step in exocytosis...
  36. ncbi request reprint SNARE assembly and disassembly exhibit a pronounced hysteresis
    Dirk Fasshauer
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Struct Biol 9:144-51. 2002
    ..We suggest that the hysteresis is a hallmark of all SNARE complexes and that complex assembly and disassembly follow different pathways that may be independently controlled...
  37. ncbi request reprint Distinct kinetic changes in neurotransmitter release after SNARE protein cleavage
    Takeshi Sakaba
    Department of Neurobiology and Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Göttingen 37077, Germany
    Science 309:491-4. 2005
    ..When stimulating release by calcium influx through channels, a difference between BoNT/C1 and TeNT emerged, which suggests that cleavage of synaptobrevin modifies the coupling between channels and release-competent vesicles...
  38. pmc Structure parameters of synaptic vesicles quantified by small-angle x-ray scattering
    Simon Castorph
    Institut fur Rontgenphysik, Universitat Gottingen, Gottingen, Germany
    Biophys J 98:1200-8. 2010
    ..Not only does our model confirm many of the preexisting ideas concerning SV structure, but also for the first time, to our knowledge, it indicates structural refinements, such as the presence of protein microdomains...
  39. pmc Synaptic vesicles are constitutively active fusion machines that function independently of Ca2+
    Matthew Holt
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Curr Biol 18:715-22. 2008
    ..To bridge this gap, we have used purified synaptic vesicles, together with artificial membranes containing core-constituted SNAREs as reaction partners, in fusion assays...
  40. ncbi request reprint The riddle of the Sec1/Munc-18 proteins - new twists added to their interactions with SNAREs
    Dieter Gallwitz
    Department of Molecular Genetics, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 37070, Gottingen, Germany
    Trends Biochem Sci 28:113-6. 2003
    ..Amazing as this might be for those considering the evolution of conserved folds, it leaves the question of how SM proteins operate in membrane fusion unanswered...
  41. pmc SNAREs in native plasma membranes are active and readily form core complexes with endogenous and exogenous SNAREs
    Thorsten Lang
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, D 37077 Gottingen, Germany
    J Cell Biol 158:751-60. 2002
    ..We conclude that the activity of membrane-resident SNAREs is not downregulated by control proteins but is constitutively active even if not engaged in fusion events...
  42. pmc Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules
    Dietmar Riedel
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Cell Biol 22:6487-97. 2002
    ..We conclude that Rab3D exerts its function during granule maturation, possibly by preventing homotypic fusion of secretory granules...
  43. pmc Imaging direct, dynamin-dependent recapture of fusing secretory granules on plasma membrane lawns from PC12 cells
    Phillip Holroyd
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 99:16806-11. 2002
    ....
  44. doi request reprint Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis
    Nathan J Pavlos
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Neurosci 30:13441-53. 2010
    ..Finally, we demonstrate that selective modulation of the GTP/GDP switch mechanism of Rab27b impairs SV recycling, suggesting that Rab27b, probably in concert with Rab3s, is involved in SV exocytosis...
  45. pmc SNARE function is not involved in early endosome docking
    Ulf Geumann
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Göttingen 37077, Germany
    Mol Biol Cell 19:5327-37. 2008
    ..We conclude that docking of early endosomes is independent of SNARE function...
  46. ncbi request reprint STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis
    Katrin I Willig
    Departments of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nature 440:935-9. 2006
    ..Our study also demonstrates that questions involving cellular structures with dimensions of a few tens of nanometres can be resolved with conventional far-field optics and visible light...
  47. ncbi request reprint Kiss-and-run, collapse and 'readily retrievable' vesicles
    Silvio O Rizzoli
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Traffic 8:1137-44. 2007
    ....
  48. doi request reprint Complexins living up to their name--new light on their role in exocytosis
    Alexander Stein
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Neuron 64:295-7. 2009
    ..In a study by Xue et al. in this issue of Neuron, contradictory data from Drosophila and mouse complexin mutants have been resolved, revealing a complex pattern of facilitatory and inhibitory domains...
  49. doi request reprint Synaptic membrane proteins form stable microdomains in early endosomes
    Ulf Geumann
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Göttingen 37077, Germany
    Microsc Res Tech 73:606-17. 2010
    ..We conclude that synaptic membrane proteins are organized in stable but dynamic clusters within endosomes, which are likely to persist during membrane recycling...
  50. ncbi request reprint Synaptotagmin activates membrane fusion through a Ca2+-dependent trans interaction with phospholipids
    Alexander Stein
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Nat Struct Mol Biol 14:904-11. 2007
    ..However, our findings also show that the action of synaptotagmin on the fusion-arrested state of docked vesicles in vivo is not fully reproduced in vitro...
  51. pmc The specificity of SNARE pairing in biological membranes is mediated by both proof-reading and spatial segregation
    Ioanna Bethani
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    EMBO J 26:3981-92. 2007
    ....
  52. ncbi request reprint A stable interaction between syntaxin 1a and synaptobrevin 2 mediated by their transmembrane domains
    M Margittai
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    FEBS Lett 446:40-4. 1999
    ..Furthermore, the complex is formed when a truncated version of syntaxin is used that contains only 12 additional amino acid residues outside the membrane anchor. We conclude that the interaction is mediated by the transmembrane domains...
  53. pmc Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies
    Daniel Zwilling
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    EMBO J 26:9-18. 2007
    ..We conclude that homotypic fusion reactions may proceed with multiple SNARE topologies, suggesting that the conserved SNARE structure allows for flexibility in the initial interactions needed for fusion...
  54. ncbi request reprint Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles
    Silvio O Rizzoli
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 37077 Gottingen, Germany
    Traffic 7:1163-76. 2006
    ....
  55. pmc Helical extension of the neuronal SNARE complex into the membrane
    Alexander Stein
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nature 460:525-8. 2009
    ..Our results suggest that the final phase of SNARE assembly is directly coupled to membrane merger...
  56. pmc Discrimination between docking and fusion of liposomes reconstituted with neuronal SNARE-proteins using FCS
    Anna Cypionka
    Department of Neurobiology, AG Biomolecular Spectroscopy and Single Molecule Detection, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 106:18575-80. 2009
    ..Our results show that under appropriate conditions a docked state, mediated by trans-SNARE interactions, can be isolated that constitutes an intermediate in the fusion pathway...
  57. doi request reprint Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein
    Mads Grønborg
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Neurosci 30:2-12. 2010
    ....
  58. pmc Identification of functionally interacting SNAREs by using complementary substitutions in the conserved '0' layer
    Carmen T Graf
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Biol Cell 16:2263-74. 2005
    ..This rescue is attributed to the involvement of Sed5p and Ykt6p in a different SNARE complex that functions in intra-Golgi trafficking...
  59. ncbi request reprint The Habc domain and the SNARE core complex are connected by a highly flexible linker
    Martin Margittai
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Biochemistry 42:4009-14. 2003
    ..We conclude that the linker region exhibits a high degree of conformational flexibility...
  60. ncbi request reprint Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression
    Matthew Holt
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Biol Chem 281:17076-83. 2006
    ..However, neither complex assembly nor fusion was as efficient as with SNAP-25...
  61. pmc The Ca2+ affinity of synaptotagmin 1 is markedly increased by a specific interaction of its C2B domain with phosphatidylinositol 4,5-bisphosphate
    Anand Radhakrishnan
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Biol Chem 284:25749-60. 2009
    ..Together, our findings lend support to the view that synaptotagmin functions by binding in a trans configuration whereby the C2A domain binds to the synaptic vesicle and the C2B binds to the PI(4,5)P2-enriched plasma membrane...
  62. doi request reprint A tethering complex recruits SNAREs and grabs vesicles
    Hans Dieter Schmitt
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Cell 139:1053-5. 2009
    ..2009) now provide evidence that the yeast Dsl1 complex tethers vesicles to the endoplasmic reticulum (ER) by binding ER SNARE proteins at its base and capturing vesicles using a loop region that extends 20 nm from the ER membrane...
  63. doi request reprint Video-rate far-field optical nanoscopy dissects synaptic vesicle movement
    Volker Westphal
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen 37077, Germany
    Science 320:246-9. 2008
    ..Our study demonstrates the emerging ability of optical microscopy to investigate intracellular physiological processes on the nanoscale in real time...
  64. doi request reprint A fluorescence-based in vitro assay for investigating early endosome dynamics
    Sina V Barysch
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Nat Protoc 5:1127-37. 2010
    ..The in vitro reactions can then be performed separately (approximately 1 d) and are conveniently carried out with multiple samples in parallel. The assay can be adapted for studying the dynamics of organelles other than endosomes...
  65. doi request reprint Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking
    Ioanna Bethani
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Traffic 10:1543-59. 2009
    ..We conclude that SNAREs are expressed at much higher levels than needed for maintenance of organelle fusion, and that loss of SNAREs is compensated for by the co-regulation of the docking machinery...
  66. pmc Sorting in early endosomes reveals connections to docking- and fusion-associated factors
    Sina V Barysch
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Göttingen 37077, Germany
    Proc Natl Acad Sci U S A 106:9697-702. 2009
    ..We conclude that fusion, docking, and sorting/budding are interconnected at the molecular level...
  67. pmc One SNARE complex is sufficient for membrane fusion
    Geert van den Bogaart
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Nat Struct Mol Biol 17:358-64. 2010
    ..Our findings shed new light on the mechanism of SNARE-mediated membrane fusion and call for a revision of current views of fusion events such as the fast release of neurotransmitters...
  68. pmc Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity
    Dorothea Brandhorst
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 103:2701-6. 2006
    ..We conclude that the specificity of SNARE pairing does not suffice to determine the specificity of organelle fusion...
  69. ncbi request reprint Fusion of vacuoles--where are the membranes, and where are the holes?
    Thorsten Lang
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Dev Cell 2:257-9. 2002
    ..During fusion, a disk of boundary membrane is excised and left behind within the fused vacuoles...
  70. ncbi request reprint Molecular anatomy of a trafficking organelle
    Shigeo Takamori
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Cell 127:831-46. 2006
    ....
  71. ncbi request reprint Plasmalemmal phosphatidylinositol-4,5-bisphosphate level regulates the releasable vesicle pool size in chromaffin cells
    Ira Milosevic
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    J Neurosci 25:2557-65. 2005
    ....
  72. ncbi request reprint Synaptic and vesicular co-localization of the glutamate transporters VGLUT1 and VGLUT2 in the mouse hippocampus
    Etienne Herzog
    Max Planck Institut fur experimentelle Medizin, Abteilung Molekulare Neurobiologie, Gottingen, Germany
    J Neurochem 99:1011-8. 2006
    ..As the number of transporter molecules per vesicle affects quantal size, the developmental window where VGLUT1 and VGLUT2 are co-expressed may allow for greater plasticity in the control of quantal release...
  73. ncbi request reprint Localization of the mouse 5-hydroxytryptamine(1A) receptor in lipid microdomains depends on its palmitoylation and is involved in receptor-mediated signaling
    Ute Renner
    Abteilung Neuro und Sinnesphysiologie, Physiologisches Institut, Universitat Gottingen, Gottingen, Germany
    Mol Pharmacol 72:502-13. 2007
    ..More importantly, the raft localization of the 5-HT1A receptor seems to be involved in receptor-mediated signaling...
  74. ncbi request reprint Endobrevin/VAMP8 mediates exocytotic release of hexosaminidase from rat basophilic leukaemia cells
    Undine Lippert
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    FEBS Lett 581:3479-84. 2007
    ..Thus, VAMP8, which also mediates fusion of late endosomes and lysosomes, plays a major role in hexosaminidase release, strengthening the view that mast cell granules share properties of both secretory granules and lysosomes...
  75. pmc Munc18-bound syntaxin readily forms SNARE complexes with synaptobrevin in native plasma membranes
    Felipe E Zilly
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    PLoS Biol 4:e330. 2006
    ..We conclude that Munc18-1 allows for the formation of a complex between syntaxin and SNAP-25 that serves as an acceptor for vesicle-bound synaptobrevin and that thus represents an intermediate in the pathway towards exocytosis...
  76. doi request reprint A sandwich enzyme-linked immunosorbent assay for the quantification of insoluble membrane and scaffold proteins
    Constanze Geumann
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Anal Biochem 402:161-9. 2010
    ..The assay is versatile, allows parallel processing and automation, and should be applicable to a wide range of hitherto inaccessible proteins...
  77. pmc Loss of the zymogen granule protein syncollin affects pancreatic protein synthesis and transport but not secretion
    Wolfram Antonin
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry 37077 Göttingen, Germany
    Mol Cell Biol 22:1545-54. 2002
    ..Furthermore, the rates of both protein synthesis and intracellular transport of secretory proteins were reduced. We conclude that syncollin plays a role in maturation and/or concentration of zymogens in zymogen granules...
  78. ncbi request reprint SNAREs prefer liquid-disordered over "raft" (liquid-ordered) domains when reconstituted into giant unilamellar vesicles
    Kirsten Bacia
    Dresden University of Technology, Institute of Biophysics, Tatzberg 47 51, 01307 Dresden, Germany
    J Biol Chem 279:37951-5. 2004
    ....
  79. ncbi request reprint A complete genetic analysis of neuronal Rab3 function
    Oliver M Schlüter
    Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
    J Neurosci 24:6629-37. 2004
    ..Furthermore, our data demonstrate that Rab3 is not in itself essential for synaptic membrane traffic but functions to modulate the basic release machinery...
  80. pmc Rab3D regulates a novel vesicular trafficking pathway that is required for osteoclastic bone resorption
    Nathan J Pavlos
    Unit of Orthopaedics, School of Surgery and Pathology, University of Western Australia, 2nd Floor M Block, QEII Medical Centre, Nedlands, Perth, Western Australia 6009, Australia
    Mol Cell Biol 25:5253-69. 2005
    ..Collectively, our data suggest that Rab3D modulates a post-TGN trafficking step that is required for osteoclastic bone resorption...
  81. ncbi request reprint A broken alpha -helix in folded alpha -Synuclein
    Sreeganga Chandra
    Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
    J Biol Chem 278:15313-8. 2003
    ..The structural organization of the alpha-helices of alpha-synuclein was not anticipated by sequence analyses and may be important for its pathogenic role...
  82. ncbi request reprint Endocytosis in neurons: editorial introduction and overview
    Reinhard Jahn
    Traffic 8:1121-2. 2007
  83. ncbi request reprint [Progress in study of molecular mechanisms of exocytosis]
    Shigeo Takamori
    21st Century COE Program, Department of Neurology and Neurological Science, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Seikagaku 79:879-82. 2007
  84. ncbi request reprint Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification
    Judyta Juranek
    Department of Neurobiology, MPI for Biophysical Chemistry, 37077 Göttingen Germany
    Eur J Neurosci 24:3043-52. 2006
    ..We conclude that Bassoon and ELKS2 perform independent and specialized functions in synaptic transmission of autonomic synapses...
  85. ncbi request reprint Divergent functions of neuronal Rab11b in Ca2+-regulated versus constitutive exocytosis
    Mikhail V Khvotchev
    Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
    J Neurosci 23:10531-9. 2003
    ....
  86. pmc Rabphilin regulates SNARE-dependent re-priming of synaptic vesicles for fusion
    Ferenc Deak
    Center for Basic Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390 9111, USA
    EMBO J 25:2856-66. 2006
    ....
  87. ncbi request reprint Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence
    Sandra Winter
    AG Funktionelle Zellbiologie, Centrum für Anatomie, Charite Universitatsmedizin Berlin, D 10115 Berlin, Germany
    J Neurosci 25:4672-80. 2005
    ..Thus, Galpha(o2) acts on a putative regulatory chloride binding domain that appears to modulate transport activity of vesicular glutamate transporters...
  88. ncbi request reprint Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion
    Oliver M Schlüter
    Max Planck Institut fur experimentelle Medizin, 37075 Gottingen, Germany
    J Biol Chem 277:40919-29. 2002
    ..These results offer an hypothesis that reconciles Rab3 transfection and knock-out studies by suggesting that Rab3 functions as a gatekeeper of a late stage in exocytosis...
  89. pmc Two-color far-field fluorescence nanoscopy
    Gerald Donnert
    Biophys J 92:L67-9. 2007
    ..The joint improvement of resolution and colocalization demonstrates the emerging potential of far-field fluorescence nanoscopy to study the spatial organization of macromolecules in cells...
  90. pmc CASK Functions as a Mg2+-independent neurexin kinase
    Konark Mukherjee
    Department of Neuroscience, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390 9111, USA
    Cell 133:328-39. 2008
    ..Moreover, our study suggests that other pseudokinases (10% of the kinome) could also be catalytically active...
  91. pmc Molecular cloning and functional characterization of human vesicular glutamate transporter 3
    Shigeo Takamori
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    EMBO Rep 3:798-803. 2002
    ....
  92. pmc Macromolecular-scale resolution in biological fluorescence microscopy
    Gerald Donnert
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 103:11440-5. 2006
    ..The reported performance of diffraction-unlimited fluorescence microscopy opens up a pathway for addressing fundamental problems in the life sciences...