Research Topics
Genomes and Genes | Karl Erich JaegerSummaryCountry: Germany Publications
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Publications
Mechanism of acetaldehyde-induced deactivation of microbial lipasesBenjamin Franken
Institute of Molecular Enzyme Technology, Heinrich Heine University Dusseldorf, Forschungszentrum Julich GmbH, D 52426 Jülich, Germany
BMC Biochem 12:10. 2011..Previous studies showed that several lipases were sensitive toward acetaldehyde deactivation whereas others were insensitive; however, a general explanation of the acetaldehyde-induced inactivation mechanism is missing...
Lipases for biotechnologyKarl Erich Jaeger
Institute for Molecular Enzyme Technology, Heinrich Heine Universitat Dusseldorf, Forschungszentrum Julich, D 52425, Julich, Germany
Curr Opin Biotechnol 13:390-7. 2002..Furthermore, novel biotechnological applications have been successfully established using lipases for the synthesis of biopolymers and biodiesel, the production of enantiopure pharmaceuticals, agrochemicals, and flavour compounds...- Enantioselective biocatalysis optimized by directed evolutionKarl Erich Jaeger
Institut für Molekulare Enzymtechnologie, Heinrich Heine Universität Duesseldorf, Forschungszentrum Juelich, D 52426 Juelich, Germany
Curr Opin Biotechnol 15:305-13. 2004.... 
A T7 RNA polymerase-based toolkit for the concerted expression of clustered genesSolmaz Arvani
Institut für Molekulare Enzymtechnologie, Heinrich Heine Universitat Dusseldorf, Forschungszentrum Julich, D 52426 Jülich, Germany
J Biotechnol 159:162-71. 2012..Our findings clearly indicate that due to its unique properties T7 RNA polymerase can be applied for overexpression of large and complex bacterial gene regions...- Structural basis for the slow dark recovery of a full-length LOV protein from Pseudomonas putidaFranco Circolone
Institut für Molekulare Enzymtechnologie, Heinrich Heine Universitat Dusseldorf, D 52426 Jülich, Germany
J Mol Biol 417:362-74. 2012..The overall structural arrangement of PpSB1-LOV, together with a complementary phylogenetic analysis, highlights a common ancestry of bacterial LOV photoreceptors and Per-ARNT-Sim chemosensors... - A novel T7 RNA polymerase dependent expression system for high-level protein production in the phototrophic bacterium Rhodobacter capsulatusNadine Katzke
Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Forschungszentrum Juelich, Stetternicher Forst, D 52426 Juelich, Germany
Protein Expr Purif 69:137-46. 2010..capsulatus cultures. This result clearly indicates that the novel R. capsulatus-based expression system is well suited for the high-level expression of soluble proteins... - LOVely enzymes - towards engineering light-controllable biocatalystsUlrich Krauss
Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Centre Juelich, D 52426 Juelich, Germany
Microb Biotechnol 3:15-23. 2010..Hence, it appears that fusion of LOV photoreceptors to functional enzyme target sites via appropriate linker structures may represent a straightforward strategy to design light controllable biocatalysts... - Lights on and action! Controlling microbial gene expression by lightThomas Drepper
Institute of Molecular Enzyme Technology, Heinrich Heine University Dusseldorf, Forschungszentrum Julich, Stetternicher Forst, 52426, Julich, Germany
Appl Microbiol Biotechnol 90:23-40. 2011.... - Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolutionThorsten Eggert
Institut für Molekulare Enzymtechnologie, Heinrich Heine Universitat Dusseldorf, Forschungszentrum Julich, 52426 Julich, Germany
Chembiochem 6:1062-7. 2005.... - Flavin mononucleotide-based fluorescent reporter proteins outperform green fluorescent protein-like proteins as quantitative in vivo real-time reportersThomas Drepper
Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Center Juelich, Stetternicher Forst, D 52426 Juelich, Germany
Appl Environ Microbiol 76:5990-4. 2010..Instead, flavin mononucleotide (FMN)-binding fluorescent proteins (FbFPs) are suitable for quantitative real-time in vivo assays under these conditions... - Cofactor trapping, a new method to produce flavin mononucleotideUlrich Krauss
Institut für Molekulare Enzymtechnologie, Heinrich Heine Universitat Dusseldorf, Forschungszentrum Julich, D 52426 Jülich, Germany
Appl Environ Microbiol 77:1097-100. 2011..This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli. This, in turn, allows the isolation of highly pure FMN... - Synthesis of chiral cyanohydrins by recombinant Escherichia coli cells in a micro-aqueous reaction systemKathrin Emmi Scholz
Institut für Molekulare Enzymtechnologie, Heinrich Heine Universität Düsseldorf and Forschungszentrum Jülich, Julich, Germany
Appl Environ Microbiol 78:5025-7. 2012..Microscopy studies employing a fusion of AtHNL with a flavin-based fluorescent protein (FbFP) reveal that the cells remain intact in the reaction system... - Distribution and phylogeny of light-oxygen-voltage-blue-light-signaling proteins in the three kingdoms of lifeUlrich Krauss
Institut für Molekulare Enzymtechnologie, Heinrich Heine Universitat Dusseldorf, Forschungszentrum Julich, Julich, Germany
J Bacteriol 191:7234-42. 2009.... - Rapid sequence scanning mutagenesis using in silico oligo design and the Megaprimer PCR of whole plasmid method (MegaWHOP)Ulrich Krauss
Institute of Molecular Enzyme Technology, Research Centre Julich, Heinrich Heine University, Dusseldorf, Julich, Germany
Methods Mol Biol 634:127-35. 2010.... - Enlightened enzymes: strategies to create novel photoresponsive proteinsUlrich Krauss
Institute of Molecular Enzyme Technology, Heinrich Heine University Dusseldorf, Forschungszentrum Julich, Julich, Germany
Chemistry 17:2552-60. 2011..Recent cell biological examples will be highlighted and possible biotechnological applications will be presented... - Combination of computational prescreening and experimental library construction can accelerate enzyme optimization by directed evolutionSusanne Aileen Funke
, , , Germany
Protein Eng Des Sel 18:509-14. 2005..Both QM/MM-based calculations and molecular biology experiments identify histidine 76 as a residue that strongly affects the catalytic activity. The experiments demonstrate its important influence on enantioselectivity... - Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and heterologous signal peptidesChristian Degering
Institute of Molecular Enzyme Technology, Heinrich Heine Universitat Dusseldorf, Research Center Julich, Julich, Germany
Appl Environ Microbiol 76:6370-6. 2010.... - The subcellular localization of a C-terminal processing protease in Pseudomonas aeruginosaRien Hoge
Research Centre Juelich, Institute for Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Juelich, Germany
FEMS Microbiol Lett 316:23-30. 2011..Our results provide experimental evidence for the generally accepted hypothesis that CTPs are located in the periplasmic space of Gram-negative bacteria... - Novel broad host range shuttle vectors for expression in Escherichia coli, Bacillus subtilis and Pseudomonas putidaSonja Christina Troeschel
Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Center Juelich, D 52426 Juelich, Germany
J Biotechnol 161:71-9. 2012..subtilis and cutinase from the eukaryotic fungus Fusarium solani pisi in three different host strains. Additionally, we report here the construction of a T7 RNA polymerase-based expression strain of P. putida... - Lipase LipC affects motility, biofilm formation and rhamnolipid production in Pseudomonas aeruginosaFrank Rosenau
Institute for Molecular Enzyme Technology, Research Centre Juelich, Heinrich Heine University Duesseldorf, Juelich, Germany
FEMS Microbiol Lett 309:25-34. 2010..Also, the P. aeruginosa lipC mutant showed a significantly altered biofilm architecture. Proteome analysis revealed the accumulation of the response regulator protein PhoP in the lipC mutant... - Biocatalytic production of enantiopure cyclohexane-trans-1,2-diol using extracellular lipases from Bacillus subtilisJean Detry
, , , Germany
Appl Microbiol Biotechnol 72:1107-16. 2006.... - Extracellular lipases from Bacillus subtilis: regulation of gene expression and enzyme activity by amino acid supply and external pHThorsten Eggert
, , Forschungszentrum Juelich, D-52426 Juelich, Germany
FEMS Microbiol Lett 225:319-24. 2003..The expression of lipA was repressed by high amino acid concentrations, whereas the lipB gene expression remained unaffected... - Novel biocatalysts for white biotechnologyThomas Drepper
Institute of Molecular Enzyme Technology, , , , Germany
Biotechnol J 1:777-86. 2006..Furthermore, we have isolated and characterized biocatalysts relevant for the preparation of enantiopure compounds... - Reporter proteins for in vivo fluorescence without oxygenThomas Drepper
Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Center Juelich, Stetternicher Forst, D 52426 Juelich, Germany
Nat Biotechnol 25:443-5. 2007.... - Mutual exchange of kinetic properties by extended mutagenesis in two short LOV domain proteins from Pseudomonas putidaKatrin Jentzsch
Institut für Molekulare Enzymtechnologie, Heinrich Heine Universitat Dusseldorf, FZ Julich, Stetternicher Forst D 52426 Jülich, Germany
Biochemistry 48:10321-33. 2009.... - The autotransporter esterase EstA of Pseudomonas aeruginosa is required for rhamnolipid production, cell motility, and biofilm formationSusanne Wilhelm
Institute for Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Centre Juelich, Stetternicher Forst, D 52426 Juelich, Germany
J Bacteriol 189:6695-703. 2007..None of the mutant phenotypes could be complemented by expression of EstA*, demonstrating that the phenotypes affected by the estA mutation depend on the enzymatically active protein... - A novel transposon for functional expression of DNA librariesChristian Leggewie
, , Forschungszentrum Juelich, D-52426 Juelich, Germany
J Biotechnol 123:281-7. 2006..Furthermore, this transposon allows the bidirectional sequencing of the respective clones starting from unique primer binding sites... - Lipase-specific foldasesFrank Rosenau
, , , , Germany
Chembiochem 5:152-61. 2004.... - Pseudomonas aeruginosa lectin LecB is located in the outer membrane and is involved in biofilm formationDenis Tielker
, , Forschungszentrum Juelich, D-52426 Juelich, Germany
Microbiology 151:1313-23. 2005..Staining of biofilm cells using fluorescently labelled LecB confirmed the presence of these ligands... - Functional cell-surface display of a lipase-specific chaperoneSusanne Wilhelm
Institute of Molecular Enzyme Technology, Heinrich Heine University Dusseldorf, Research Centre Julich, 52426 Julich, Germany
Chembiochem 8:55-60. 2007..The foldase autodisplay system reported here can be used for a variety of applications including the ultrahigh-throughput screening of large libraries of foldase variants generated by directed evolution... - Learning from directed evolution: Further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivityManfred T Reetz
Max Planck Institut fur Kohlenforschung, Kaiser Wilhelm Platz 1, 45470 Mulheim Ruhr, Germany
Chembiochem 8:106-12. 2007..This study supports our original postulate regarding the relay mechanism, offers further mechanistic insight into the role of individual mutations, and provides mutants that display even higher enantioselectivity (E of up to 64)... - Learning from directed evolution: theoretical investigations into cooperative mutations in lipase enantioselectivityMarco Bocola
, Kaiser-Wilhelm-Platz 1, , Germany
Chembiochem 5:214-23. 2004..The simulations provide insight into remote and cooperative effects of mutations... - Select the best: novel biocatalysts for industrial applicationsMonika Konarzycka-Bessler
Institute of Molecular Enzyme Technology, Heinrich-Heine University Duesseldorf, Research Centre Juelich, D-52428 Juelich, Germany
Trends Biotechnol 24:248-50. 2006.... - Prospecting for biocatalysts and drugs in the genomes of non-cultured microorganismsWolfgang R Streit
Institut fur Mikrobiologie und Genetik, Universitat Gottingen, Grisebachstrasse 8, D 37077 Gottingen, Germany
Curr Opin Biotechnol 15:285-90. 2004..So far, metagenome-based approaches have led to the isolation of many novel biocatalysts and a variety of other molecules with a high potential for downstream applications... - Identification of novel benzoylformate decarboxylases by growth selectionHelge Henning
Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Centre Juelich, , Germany
Appl Environ Microbiol 72:7510-7. 2006.... - Lectin-based affinity tag for one-step protein purificationDenis Tielker
Heinrich-Heine-University Duesseldorf, Juelich, Germany
Biotechniques 41:327-32. 2006..In conclusion, our results indicate that the lectin LecB of P. aeruginosa can be used as a tag for the high-yield one-step purification of recombinant proteins... - Conformational analysis of the blue-light sensing protein YtvA reveals a competitive interface for LOV-LOV dimerization and interdomain interactionsValentina Buttani
Dept of Physics, University of Parma, via G P Usberti 7 A, 43100 Parma, Italy
Photochem Photobiol Sci 6:41-9. 2007..The data uncover a common surface for LOV-LOV and intraprotein interaction, involving the central beta-scaffold, and offer hints to investigate the molecular basis of light-activation and regulation in LOV proteins... - A generic system for the Escherichia coli cell-surface display of lipolytic enzymesStefan Becker
, , , Grisebachstrasse 8, , Germany
FEBS Lett 579:1177-82. 2005..EstA provides a useful tool for surface display of lipases including variant libraries generated by directed evolution thereby enabling the identification of novel enzymes with interesting biological and biotechnological ramifications... - Ultrahigh-throughput screening to identify E. coli cells expressing functionally active enzymes on their surfaceStefan Becker
Department of Biochemistry, Clemens Schöpf Institute, Darmstadt University of Technology, Petersenstrasse 22, Darmstadt, Germany
Chembiochem 8:943-9. 2007..This strategy of covalently attaching a biotin label to esterase-proficient bacteria might open new avenues to ultrahigh-throughput screening of enzyme libraries for hydrolytic enzymes with enhanced activities or enantioselectivities... - A calcium-gated lid and a large beta-roll sandwich are revealed by the crystal structure of extracellular lipase from Serratia marcescensReto Meier
Department of Chemistry and Biochemistry, University Bern Freiestrasse 3, CH 3012 Bern, Switzerland
J Biol Chem 282:31477-83. 2007..The analysis of the properties of the beta-roll domains suggests an intramolecular chaperone function... - Mutations towards enantioselectivity adversely affect secretion of Pseudomonas aeruginosa lipaseSascha Hausmann
Research Centre Juelich, Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Juelich, Germany
FEMS Microbiol Lett 282:65-72. 2008..We report here the identification of two amino acid substitutions located on the protein surface, which significantly impair lipase secretion... - Hexadecane and Tween 80 stimulate lipase production in Burkholderia glumae by different mechanismsBouke K H L Boekema
Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
Appl Environ Microbiol 73:3838-44. 2007..In conclusion, hexadecane and Tween 80 enhance lipase production in B. glumae, and they act via different mechanisms... - Single-cell high-throughput screening to identify enantioselective hydrolytic enzymesStefan Becker
Institute for Biochemistry and Organic Chemistry, , Petersenstrasse 22, 64287 Darmstadt, Germany
Angew Chem Int Ed Engl 47:5085-8. 2008