Zoltan Ivics

Summary

Country: Germany

Publications

  1. pmc Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers
    Orsolya Kolacsek
    Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University and National Blood Center, Budapest, Hungary
    Mob DNA 2:5. 2011
  2. pmc Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Robert Rössle Str, 13092 Berlin, Germany
    Genome Biol 8:S1. 2007
  3. pmc Novel strong tissue specific promoter for gene expression in human germ cells
    Denis Kuzmin
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
    BMC Biotechnol 10:58. 2010
  4. doi request reprint Nonviral gene delivery with the sleeping beauty transposon system
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin 13125, Germany
    Hum Gene Ther 22:1043-51. 2011
  5. ncbi request reprint Targeted Sleeping Beauty transposition in human cells
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Mol Ther 15:1137-44. 2007
  6. pmc Sleeping Beauty transposon mutagenesis of the rat genome in spermatogonial stem cells
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Methods 53:356-65. 2011
  7. pmc The expanding universe of transposon technologies for gene and cell engineering
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Mob DNA 1:25. 2010
  8. pmc Generating knockout rats by transposon mutagenesis in spermatogonial stem cells
    Zsuzsanna Izsvák
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Methods 7:443-5. 2010
  9. pmc Transposon-mediated genome manipulation in vertebrates
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Methods 6:415-22. 2009
  10. ncbi request reprint Transposons for gene therapy!
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Curr Gene Ther 6:593-607. 2006

Collaborators

Detail Information

Publications40

  1. pmc Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers
    Orsolya Kolacsek
    Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University and National Blood Center, Budapest, Hungary
    Mob DNA 2:5. 2011
    ..abstract:..
  2. pmc Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Robert Rössle Str, 13092 Berlin, Germany
    Genome Biol 8:S1. 2007
    ....
  3. pmc Novel strong tissue specific promoter for gene expression in human germ cells
    Denis Kuzmin
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
    BMC Biotechnol 10:58. 2010
    ..We report a novel, tissue-specific promoter that was identified and engineered from the native upstream regulatory region of the human gene NDUFV1 containing an endogenous retroviral sequence...
  4. doi request reprint Nonviral gene delivery with the sleeping beauty transposon system
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin 13125, Germany
    Hum Gene Ther 22:1043-51. 2011
    ....
  5. ncbi request reprint Targeted Sleeping Beauty transposition in human cells
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Mol Ther 15:1137-44. 2007
    ..Targeted transposition can be a powerful technology for safe transgene integration in human therapeutic applications...
  6. pmc Sleeping Beauty transposon mutagenesis of the rat genome in spermatogonial stem cells
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Methods 53:356-65. 2011
    ....
  7. pmc The expanding universe of transposon technologies for gene and cell engineering
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Mob DNA 1:25. 2010
    ....
  8. pmc Generating knockout rats by transposon mutagenesis in spermatogonial stem cells
    Zsuzsanna Izsvák
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Methods 7:443-5. 2010
    ..Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat...
  9. pmc Transposon-mediated genome manipulation in vertebrates
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Methods 6:415-22. 2009
    ..Here we provide an overview of transposon-based methods used in vertebrate model organisms with an emphasis on the mouse system and highlight the most important considerations concerning genetic applications of the transposon systems...
  10. ncbi request reprint Transposons for gene therapy!
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Curr Gene Ther 6:593-607. 2006
    ....
  11. doi request reprint Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cells
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Protoc 6:1521-35. 2011
    ..The complete protocol from transfection of SSCs to the genotyping of heterozygous F(1) offspring that harbor genomic SB gene-trap insertions takes 5-6 months...
  12. ncbi request reprint The Sleeping Beauty transposable element: evolution, regulation and genetic applications
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13092, Berlin, Germany
    Curr Issues Mol Biol 6:43-55. 2004
    ....
  13. pmc Genomic parasites and genome evolution
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13092 Berlin, Germany
    Genome Biol 10:306. 2009
    ..A report of the Second International Conference/Workshop on the Genomic Impact of Eukaryotic Transposable Elements, Pacific Grove, USA, 6-10 February 2009...
  14. ncbi request reprint A whole lotta jumpin' goin' on: new transposon tools for vertebrate functional genomics
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Trends Genet 21:8-11. 2005
    ..Two recent articles report on the construction of retrotransposable elements that efficiently 'jump' in mammalian cells. These new elements hold great promise as useful vectors for insertional mutagenesis screens in the mouse...
  15. pmc Retargeting sleeping beauty transposon insertions by engineered zinc finger DNA-binding domains
    Katrin Voigt
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Mol Ther 20:1852-62. 2012
    ..Improvements in ZF technology and a careful choice of targeted genomic regions may improve the safety profile of SB for future clinical applications...
  16. pmc Retargeting transposon insertions by the adeno-associated virus Rep protein
    Ismahen Ammar
    Max Delbruck Center for Molecular Medicine, 13125 Berlin, Germany
    Nucleic Acids Res 40:6693-712. 2012
    ..This study provides a comparative insight into target site selection properties of transposons, as well as proof-of-principle for targeted chromosomal transposition by composite protein-protein and protein-DNA interactions...
  17. ncbi request reprint Healing the wounds inflicted by sleeping beauty transposition by double-strand break repair in mammalian somatic cells
    Zsuzsanna Izsvák
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13092 Berlin, Germany
    Mol Cell 13:279-90. 2004
    ..The overlapping but distinct roles of repair factors in transposition and in V(D)J recombination might influence the outcomes of these mechanistically similar processes...
  18. pmc The Frog Prince: a reconstructed transposon from Rana pipiens with high transpositional activity in vertebrate cells
    Csaba Miskey
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, D 13092 Berlin, Germany
    Nucleic Acids Res 31:6873-81. 2003
    ..Frog Prince can greatly extend our possibilities for genetic analyses in vertebrates...
  19. pmc The DNA-bending protein HMGB1 is a cellular cofactor of Sleeping Beauty transposition
    Hatem Zayed
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, D 13092 Berlin, Germany
    Nucleic Acids Res 31:2313-22. 2003
    ....
  20. ncbi request reprint Development of hyperactive sleeping beauty transposon vectors by mutational analysis
    Hatem Zayed
    Max Delbruck Center for Molecular Medicine, Robert Rössle Strasse10, D 13092 Berlin, Germany
    Mol Ther 9:292-304. 2004
    ..The improved vector system should prove useful for efficient gene transfer in vertebrates...
  21. ncbi request reprint Transposable elements for transgenesis and insertional mutagenesis in vertebrates: a contemporary review of experimental strategies
    Zoltan Ivics
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Methods Mol Biol 260:255-76. 2004
    ..These features all contribute to the usefulness of TEs as tools for vertebrate functional genomics, as well as for animal biotechnology and human gene therapy...
  22. pmc Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes
    Ludivine Sinzelle
    Max Delbruck Center for Molecular Medicine, 13092 Berlin, Germany
    Proc Natl Acad Sci U S A 105:4715-20. 2008
    ..The Harbinger transposon will serve as a useful experimental system for transposon biology and for investigating the enzymatic functions of domesticated, transposon-derived cellular genes...
  23. ncbi request reprint Involvement of a bifunctional, paired-like DNA-binding domain and a transpositional enhancer in Sleeping Beauty transposition
    Zsuzsanna Izsvák
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, D 13092 Berlin, Germany
    J Biol Chem 277:34581-8. 2002
    ..These interactions may result in enhanced stability of synaptic complexes, which might explain the efficient transposition of Sleeping Beauty in vertebrate cells...
  24. ncbi request reprint Transcriptional activities of the Sleeping Beauty transposon and shielding its genetic cargo with insulators
    Oliver Walisko
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Mol Ther 16:359-69. 2008
    ....
  25. pmc Comparative analysis of transposable element vector systems in human cells
    Ivana Grabundzija
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Mol Ther 18:1200-9. 2010
    ..SB, Tol2, and PB constitute complementary research tools for gene transfer in mammalian cells with important implications for fundamental and translational research...
  26. doi request reprint Translating Sleeping Beauty transposition into cellular therapies: victories and challenges
    Zsuzsanna Izsvák
    Max Delbrück Center for Molecular Medicine MDC, Berlin, Germany
    Bioessays 32:756-67. 2010
    ..The first clinical application of the SB system will help to validate the safety of this approach...
  27. doi request reprint Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Genet 41:753-61. 2009
    ....
  28. pmc The ancient mariner sails again: transposition of the human Hsmar1 element by a reconstructed transposase and activities of the SETMAR protein on transposon ends
    Csaba Miskey
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, Berlin, Germany
    Mol Cell Biol 27:4589-600. 2007
    ..The novel transposon system can be a useful tool for genome manipulations in vertebrates and for investigations into the transpositional dynamics and the contributions of these elements to primate genome evolution...
  29. ncbi request reprint Efficient stable gene transfer into human cells by the Sleeping Beauty transposon vectors
    Zsuzsanna Izsvák
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Methods 49:287-97. 2009
    ..The given protocols are adaptable to any vertebrate cells in culture...
  30. doi request reprint Targeted gene insertion for molecular medicine
    Katrin Voigt
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13092, Berlin, Germany
    J Mol Med (Berl) 86:1205-19. 2008
    ....
  31. ncbi request reprint Interference with cell cycle progression by parasitic genetic elements: sleeping beauty joins the club
    Oliver Walisko
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Cell Cycle 5:1275-80. 2006
    ..Thus, interference with the cell cycle seems to be a shared strategy of parasitic selfish genetic elements...
  32. ncbi request reprint Sleeping beauty transposition: biology and applications for molecular therapy
    Zsuzsanna Izsvák
    Max Delbruck Center for Molecular Medicine, D 13092 Berlin, Germany
    Mol Ther 9:147-56. 2004
    ..In this review we summarize our current knowledge of Sleeping Beauty biology and describe the strengths and current limitations of transposon technology for gene therapeutic applications...
  33. pmc Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells
    Ivana Grabundzija
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Nucleic Acids Res 41:1829-47. 2013
    ..This strategy would allow correction of a genetic defect by site-specific insertion of a therapeutic gene construct into 'safe harbor' sites in the genomes of autologous, patient-derived iPS cells...
  34. doi request reprint The Sleeping Beauty transposon toolbox
    Ismahen Ammar
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Methods Mol Biol 859:229-40. 2012
    ..This review highlights the state-of-the-art in SB transposon technology in diverse genetic applications with special emphasis on the transposon as well as transposase vectors currently available in the SB transposon toolbox...
  35. doi request reprint Stable gene transfer and expression in cord blood-derived CD34+ hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system
    Xingkui Xue
    Division of Pediatric Blood and Marrow Transplantation, Berlin, Germany
    Blood 114:1319-30. 2009
    ..Our results support the continuing development of SB-based gene transfer into human hematopoietic stem cells as a modality for gene therapy...
  36. pmc Sleeping Beauty transposase modulates cell-cycle progression through interaction with Miz-1
    Oliver Walisko
    Max Delbruck Center for Molecular Medicine, D 13092 Berlin, Germany
    Proc Natl Acad Sci U S A 103:4062-7. 2006
    ..Because nonhomologous end-joining is required for efficient SB transposition, the transposase-induced G(1) slowdown is probably a selfish act on the transposon's part to maximize the chance for a successful transposition event...
  37. doi request reprint Insertional engineering of chromosomes with Sleeping Beauty transposition: an overview
    Ivana Grabundzija
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Methods Mol Biol 738:69-85. 2011
    ....
  38. pmc Regulation of DNA transposition by CpG methylation and chromatin structure in human cells
    Tobias Jursch
    Max Delbruck Center for Molecular Medicine, D 13125, Berlin, Germany
    Mob DNA 4:15. 2013
    ..However, very surprisingly, it was previously shown that CpG methylation of the Sleeping Beauty (SB) transposon significantly enhanced transposition in mouse embryonic stem cells...
  39. pmc A novel active endogenous retrovirus family contributes to genome variability in rat inbred strains
    Yongming Wang
    Max Delbrück Center for Molecular Medicine MDC, Berlin, Germany
    Genome Res 20:19-27. 2010
    ..This study identifies an ERV-K-type family in rats that shows obvious signs of recent activity. Ongoing retrotranspositional activity may significantly add to genomic variability among inbred rat strains...