A Jeltsch

Summary

Affiliation: International University Bremen
Country: Germany

Publications

  1. ncbi Molecular enzymology of mammalian DNA methyltransferases
    A Jeltsch
    School of Engineering and Science, International University Bremen, Germany
    Curr Top Microbiol Immunol 301:203-25. 2006
  2. ncbi On the enzymatic properties of Dnmt1: specificity, processivity, mechanism of linear diffusion and allosteric regulation of the enzyme
    Albert Jeltsch
    International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
    Epigenetics 1:63-6. 2006
  3. ncbi Application of DNA methyltransferases in targeted DNA methylation
    Albert Jeltsch
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Appl Microbiol Biotechnol 75:1233-40. 2007
  4. pmc Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation
    Da Jia
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322, USA
    Nature 449:248-51. 2007
  5. ncbi The Dnmt1 DNA-(cytosine-C5)-methyltransferase methylates DNA processively with high preference for hemimethylated target sites
    Andrea Hermann
    Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    J Biol Chem 279:48350-9. 2004
  6. ncbi Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L
    Humaira Gowher
    International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
    J Biol Chem 280:13341-8. 2005
  7. pmc Transition from nonspecific to specific DNA interactions along the substrate-recognition pathway of dam methyltransferase
    John R Horton
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Cell 121:349-61. 2005
  8. ncbi Catalytic mechanism of DNA-(cytosine-C5)-methyltransferases revisited: covalent intermediate formation is not essential for methyl group transfer by the murine Dnmt3a enzyme
    Sabine Reither
    Institut fur Biochemie, FB 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392, Giessen, Germany
    J Mol Biol 329:675-84. 2003
  9. ncbi Methylation sensitivity of restriction enzymes interacting with GATC sites
    Andrea Hermann
    Justus Liebig Universitat Giessen, Germany
    Biotechniques 34:924-6, 928, 930. 2003
  10. ncbi De novo methylation of nucleosomal DNA by the mammalian Dnmt1 and Dnmt3A DNA methyltransferases
    Humaira Gowher
    School of Engineering and Science, International University Bremen, Campus Ring 1, D 28759 Bremen, Germany
    Biochemistry 44:9899-904. 2005

Collaborators

  • Xiaodong Cheng
  • Michal Minczuk
  • F Lyko
  • Shuang yong Xu
  • D T Dryden
  • Noreen E Murray
  • Jurate Bitinaite
  • Hiroyuki Sasaki
  • ERIC SELKER
  • ARTHUR RIGGS
  • ASHOK BHAGWAT
  • Stephen E Halford
  • R J Roberts
  • Humaira Gowher
  • Philipp Rathert
  • Andrea Hermann
  • Kirsten Liebert
  • Rachna Goyal
  • John R Horton
  • Vikas Handa
  • Alfred Pingoud
  • Kristin Eisenschmidt
  • Christian Rohde
  • Xing Zhang
  • Tomasz P Jurkowski
  • Sabine Reither
  • Carsten Beck
  • Fuyang Li
  • Thomas Lanio
  • Mehrnaz Fatemi
  • Bernardina T F van der Gun
  • Raphaël Métivier
  • Tamas Rasko
  • Renata Z Jurkowska
  • Krystyna Slaska-Kiss
  • Yingying Zhang
  • Antal Kiss
  • Richard Reinhardt
  • Da Jia
  • Zheng Hua Xie
  • Guo Liang Xu
  • Jian ping Ding
  • Andras Simoncsits
  • Ying Zi Ge
  • Andreas Humeny
  • Aspasia Spyridaki
  • Sabine Urig
  • Lou F M H de Leij
  • Marianne G Rots
  • Christian Freund
  • Richard P Carmouche
  • Vladimir Benes
  • Elmar Weinhold
  • Yasuhiko Komatsu
  • Amélie Monami
  • George Reid
  • Arunkumar Dhayalan
  • David Ibberson
  • Peter Barath
  • Marie Murakami
  • Reinhold Wasserkort
  • Raluca Tamas
  • Florence Demay
  • Rene Cortese
  • Yoichi Shinkai
  • Christine Le Péron
  • Heinrich Stamerjohanns
  • Frank Gannon
  • Gilles Salbert
  • Marcel H J Ruiters
  • Rozenn Gallais
  • Renata Jurkowska
  • Pamela M J McLaughlin
  • Christophe Tiffoche
  • Monika Papworth
  • Sergei Ragozin
  • Markus Roth
  • Wolfgang Nellen
  • Heike Laser
  • Marcella Orwick
  • Sanjay Chahar
  • Nils Anspach
  • Liliya Kulishova
  • Yan Nv Huang
  • Zhao Xia Chen
  • Miklos Bekes
  • Kenichiro Hata
  • Chris J Stockdale
  • Tom Owen-Hughes
  • Stanley Hattman

Detail Information

Publications43

  1. ncbi Molecular enzymology of mammalian DNA methyltransferases
    A Jeltsch
    School of Engineering and Science, International University Bremen, Germany
    Curr Top Microbiol Immunol 301:203-25. 2006
    ..The allosteric activation of Dnmt1 for methylation at unmodified sites is described. Wherever possible, correlations between the biochemical properties of the enzymes and their physiological functions in the cell are indicated...
  2. ncbi On the enzymatic properties of Dnmt1: specificity, processivity, mechanism of linear diffusion and allosteric regulation of the enzyme
    Albert Jeltsch
    International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
    Epigenetics 1:63-6. 2006
    ..Binding of DNA to allosteric site(s) in the N-terminal part of the enzyme can lead to stimulation and inhibition of its catalytic activity depending on the nature of the substrate and effector...
  3. ncbi Application of DNA methyltransferases in targeted DNA methylation
    Albert Jeltsch
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Appl Microbiol Biotechnol 75:1233-40. 2007
    ..Thereby, gene expression can be switched off specifically, efficiently, and stably, which has a number of potential medical applications...
  4. pmc Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation
    Da Jia
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322, USA
    Nature 449:248-51. 2007
    ..These results suggest a basis for the recognition and methylation of differentially methylated regions in imprinted genes, involving the detection of both nucleosome modification and CpG spacing...
  5. ncbi The Dnmt1 DNA-(cytosine-C5)-methyltransferase methylates DNA processively with high preference for hemimethylated target sites
    Andrea Hermann
    Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    J Biol Chem 279:48350-9. 2004
    ....
  6. ncbi Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L
    Humaira Gowher
    International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
    J Biol Chem 280:13341-8. 2005
    ..Following dissociation of Dnmt3L, Dnmt3A adopts a closed conformation leading to slow rates of DNA release. Therefore, Dnmt3L acts as a substrate exchange factor that accelerates DNA and AdoMet binding to de novo DNA methyltransferases...
  7. pmc Transition from nonspecific to specific DNA interactions along the substrate-recognition pathway of dam methyltransferase
    John R Horton
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Cell 121:349-61. 2005
    ..These structures illustrate the transition in enzyme-DNA interaction from nonspecific to specific interaction, suggesting that there is a temporal order for formation of specific contacts...
  8. ncbi Catalytic mechanism of DNA-(cytosine-C5)-methyltransferases revisited: covalent intermediate formation is not essential for methyl group transfer by the murine Dnmt3a enzyme
    Sabine Reither
    Institut fur Biochemie, FB 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392, Giessen, Germany
    J Mol Biol 329:675-84. 2003
    ..We propose that correct positioning of the flipped base and the cofactor and binding to the transition state of methyl group transfer are the most important roles of the Dnmt3a enzyme in the catalytic cycle of methyl group transfer...
  9. ncbi Methylation sensitivity of restriction enzymes interacting with GATC sites
    Andrea Hermann
    Justus Liebig Universitat Giessen, Germany
    Biotechniques 34:924-6, 928, 930. 2003
  10. ncbi De novo methylation of nucleosomal DNA by the mammalian Dnmt1 and Dnmt3A DNA methyltransferases
    Humaira Gowher
    School of Engineering and Science, International University Bremen, Campus Ring 1, D 28759 Bremen, Germany
    Biochemistry 44:9899-904. 2005
    ....
  11. ncbi Reversible inactivation of the CG specific SssI DNA (cytosine-C5)-methyltransferase with a photocleavable protecting group
    Philipp Rathert
    Biochemistry Laboratory International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
    Chembiochem 8:202-7. 2007
    ..Irradiation of the inactivated enzyme with near-ultraviolet light (320-400 nm) restored 60 % of the catalytic activity. This indicates that caging by DMNBB can be used for the reversible inactivation of M.SssI...
  12. pmc Chimeric DNA methyltransferases target DNA methylation to specific DNA sequences and repress expression of target genes
    Fuyang Li
    Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    Nucleic Acids Res 35:100-12. 2007
    ..In short, we show here that it is possible to direct DNA MTase activity to predetermined sites in DNA, achieve targeted gene silencing in mammalian cell lines and interfere with HSV-1 propagation...
  13. ncbi The Escherichia coli dam DNA methyltransferase modifies DNA in a highly processive reaction
    Sabine Urig
    Institut fur Biochemie, Fachbereich Biologie, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    J Mol Biol 319:1085-96. 2002
    ....
  14. ncbi A fluorimetric assay for on-line detection of DNA cleavage by restriction endonucleases
    Kristin Eisenschmidt
    Justus Liebig Universitat, Institut für Biochemie FB08, 35392 Giessen, Germany
    J Biotechnol 96:185-91. 2002
    ..This assay can be carried out in a microplate format, which allows for the analysis of many restriction endonuclease variants in parallel...
  15. ncbi Two alternative conformations of S-adenosyl-L-homocysteine bound to Escherichia coli DNA adenine methyltransferase and the implication of conformational changes in regulating the catalytic cycle
    Kirsten Liebert
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, 28759 Bremen, Germany
    J Biol Chem 282:22848-55. 2007
    ..This suggests that the hexapeptide couples specific DNA binding (Lys(9)), AdoMet binding (Trp(10)), and insertion of the flipped target base into the active site pocket (Lys(14))...
  16. ncbi German human methylome project started
    Albert Jeltsch
    Cancer Res 66:7378. 2006
  17. ncbi Two substrates are better than one: dual specificities for Dnmt2 methyltransferases
    Albert Jeltsch
    School of Engineering and Science, International University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Trends Biochem Sci 31:306-8. 2006
    ..This finding has important implications for understanding the evolutionary relationships among these enzymes...
  18. ncbi Phosphorylation of serine-515 activates the Mammalian maintenance methyltransferase Dnmt1
    Rachna Goyal
    Institut fur Biochemie, Justus Liebig Universitat, Giessen, Germany
    Epigenetics 2:155-60. 2007
    ..We conclude that phosphorylation of Dnmt1 at Ser515 could be an important regulator of Dnmt1 activity during cell cycle and after proliferative stimuli...
  19. ncbi The M.EcoRV DNA-(adenine N6)-methyltransferase uses DNA bending for recognition of an expanded EcoDam recognition site
    Tomasz P Jurkowski
    Biochemistry Laboratory, BioRec Program, School of Engineering and Science, Jacobs University Bremen, Germany
    J Biol Chem 282:36942-52. 2007
    ..These results suggest a temporal order of the formation of protein-DNA contacts in which the Gua6-Arg128 contact forms early followed by DNA bending and, finally, the formation of the Lys11-Gua1 contact...
  20. pmc Analysis of the substrate specificity of the Dim-5 histone lysine methyltransferase using peptide arrays
    Philipp Rathert
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Chem Biol 15:5-11. 2008
    ..Comparative analyses of peptide arrays with wild-type and mutant enzymes, therefore, are well suited to investigate the target specificity of protein methyltransferases and study epigenetic crosstalk...
  21. ncbi Detection and quantitation of the activity of DNA methyltransferases using a biotin/avidin microplate assay
    Kirsten Liebert
    School of Engineering and Science, International University Bremen, Bremen, Germany
    Methods Mol Biol 418:149-56. 2008
    ..Furthermore, the assay is very convenient, fast and well suited for automation...
  22. pmc Bisulfite sequencing Data Presentation and Compilation (BDPC) web server--a useful tool for DNA methylation analysis
    Christian Rohde
    School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28725 Bremen, Germany
    Nucleic Acids Res 36:e34. 2008
    ..v) A condensed file, containing all primary data in simplified format for further downstream data analysis and (vi) a custom track file for display of the results in the UCSC genome browser...
  23. doi Cyclical DNA methylation of a transcriptionally active promoter
    Raphaël Métivier
    Universite de Rennes I, CNRS, UMR 6026 Equipe SPARTE, IFR 140 GFAS, Campus de Beaulieu, 35042 Rennes Cedex, France
    Nature 452:45-50. 2008
    ..Cyclical changes in the methylation status of promoter CpGs may thus represent a critical event in transcriptional achievement...
  24. doi Persistent downregulation of the pancarcinoma-associated epithelial cell adhesion molecule via active intranuclear methylation
    Bernardina T F van der Gun
    Department of Pathology and Laboratory Medicine, Section Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands
    Int J Cancer 123:484-9. 2008
    ..This is the first study showing that active DNA methylation leads to sustained silencing of endogenous EpCAM expression...
  25. pmc Protein lysine methyltransferase G9a acts on non-histone targets
    Philipp Rathert
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Nat Chem Biol 4:344-6. 2008
    ..We demonstrate potential downstream signaling pathways for methylation of non-histone proteins...
  26. doi Reading and writing DNA methylation
    Albert Jeltsch
    Nat Struct Mol Biol 15:1003-4. 2008
  27. ncbi Mutations in DNA methyltransferase DNMT3B in ICF syndrome affect its regulation by DNMT3L
    Zheng Hua Xie
    The State Key Laboratory of Molecular Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
    Hum Mol Genet 15:1375-85. 2006
    ....
  28. pmc Specificity of DNA triple helix formation analyzed by a FRET assay
    Sabine Reither
    Institut fur Biochemie, FB8 Justus Liebig Universität, Heinrich Buff Ring 58, 35392, Giessen, Germany
    BMC Biochem 3:27. 2002
    ..A third DNA strand can bind into the major groove of a homopurine duplex DNA to form a DNA triple helix. Sequence specific triplex formation can be applied for gene targeting, gene silencing and mutagenesis...
  29. ncbi Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA
    Mehrnaz Fatemi
    Institut fur Biochemie, Justus Liebig Universitat, Giessen, Germany
    Eur J Biochem 269:4981-4. 2002
    ..This model agrees with the biochemical properties of these enzymes and provides a mechanistic basis for the functional cooperation of different DNA MTases in de novo methylation of DNA that has also been observed in vivo...
  30. ncbi Probing the DNA interface of the EcoRV DNA-(adenine-N6)-methyltransferase by site-directed mutagenesis, fluorescence spectroscopy, and UV cross-linking
    Carsten Beck
    Institut fur Biochemie, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    Biochemistry 41:14103-10. 2002
    ..Whether the observation that evolutionarily conserved contacts are formed early during complex formation is a general rule for DNA interacting enzymes or proteins that change their specificity during evolution remains to be seen...
  31. ncbi Detection and analysis of enzymatic DNA methylation of oligonucleotide substrates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    Andreas Humeny
    Institut fur Biochemie, Emil Fischer Zentrum, Friedrich Alexander Universitat Erlangen Nurnberg, Fahrstrasse 17, 91054 Erlangen, Germany
    Anal Biochem 313:160-6. 2003
    ..We provide evidence that the dam MTase modifies DNA in a processive reaction, confirming earlier findings...
  32. pmc A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
    Richard J Roberts
    New England Biolabs, Beverly, MA 01915, USA
    Nucleic Acids Res 31:1805-12. 2003
    ..It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes...
  33. ncbi The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity
    Andrea Hermann
    Institut fur Biochemie, FB 8, Heinrich Buff Ring 58, Justus Liebig Universitat, 35392 Giessen, Germany
    J Biol Chem 278:31717-21. 2003
    ..Methylation was observed at CG sites in a loose ttnCGga(g/a) consensus sequence, suggesting that Dnmt2 has a more specialized role than other mammalian DNA methyltransferases...
  34. ncbi Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuII
    Aspasia Spyridaki
    Department of Biology, IMBB FORTH, University of Crete, PO Box 1527, Heraklion, GR 71110, Crete, Greece
    J Mol Biol 331:395-406. 2003
    ..These results, for the first time, shed light on the pathway by which metal ions as essential cofactors enter the catalytic center of restriction endonucleases...
  35. ncbi Maintenance of species identity and controlling speciation of bacteria: a new function for restriction/modification systems?
    Albert Jeltsch
    Institut fur Biochemie, FB 08, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    Gene 317:13-6. 2003
    ....
  36. ncbi Chromatin targeting of de novo DNA methyltransferases by the PWWP domain
    Ying Zi Ge
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China
    J Biol Chem 279:25447-54. 2004
    ..Our data establish the PWWP domain as a novel chromatin/chromosome-targeting module and suggest that the PWWP-mediated chromatin association is essential for the function of the de novo methyltransferases during development...
  37. ncbi Anomalous mobility of polymerase chain reaction products after bisulfite treatment of DNA
    Vikas Handa
    Institut fur Biochemie, FB 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    Anal Biochem 333:196-8. 2004
  38. ncbi Mechanism of inhibition of DNA methyltransferases by cytidine analogs in cancer therapy
    Humaira Gowher
    School of Engineering and Science, International University Bremen, Bremen 28759, Germany
    Cancer Biol Ther 3:1062-8. 2004
    ..This review provides an insight to how the chemistry of DNA methylation is involved in the performance of these drugs targeted against it...
  39. ncbi Profound flanking sequence preference of Dnmt3a and Dnmt3b mammalian DNA methyltransferases shape the human epigenome
    Vikas Handa
    Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    J Mol Biol 348:1103-12. 2005
    ..Furthermore, similar flanking sequence preferences have been found for the stimulation of the immune system by unmethylated CGs, suggesting a co-evolution of DNA MTases and the immune system...
  40. pmc Developing a programmed restriction endonuclease for highly specific DNA cleavage
    Kristin Eisenschmidt
    Institut fur Biochemie, Justus Liebig Universitat, Heinrich Buff Ring 58, D 35392 Giessen, Germany
    Nucleic Acids Res 33:7039-47. 2005
    ..Single base pair substitutions in the TFS prevent addressed DNA cleavage by scPvuII-TFO...
  41. pmc Accuracy of DNA methylation pattern preservation by the Dnmt1 methyltransferase
    Rachna Goyal
    Institut für Biochemie FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    Nucleic Acids Res 34:1182-8. 2006
    ....
  42. pmc Structure and substrate recognition of the Escherichia coli DNA adenine methyltransferase
    John R Horton
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    J Mol Biol 358:559-70. 2006
    ..3) The orphaned Thy residue displays structural flexibility by adopting an extrahelical or intrahelical position where it is in contact to N120...
  43. ncbi Beyond Watson and Crick: DNA methylation and molecular enzymology of DNA methyltransferases
    Albert Jeltsch
    Institut fur Biochemie, FB 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    Chembiochem 3:274-93. 2002
    ..In addition, the structures and mechanisms of the DNA methyltransferases, which enable them to specifically recognize their DNA targets and to induce such large conformational changes of the DNA, are discussed...