A Jeltsch

Summary

Affiliation: International University Bremen
Country: Germany

Publications

  1. pmc Chimeric DNA methyltransferases target DNA methylation to specific DNA sequences and repress expression of target genes
    Fuyang Li
    Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    Nucleic Acids Res 35:100-12. 2007
  2. pmc Accuracy of DNA methylation pattern preservation by the Dnmt1 methyltransferase
    Rachna Goyal
    Institut für Biochemie FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    Nucleic Acids Res 34:1182-8. 2006
  3. ncbi Molecular enzymology of mammalian DNA methyltransferases
    A Jeltsch
    School of Engineering and Science, International University Bremen, Germany
    Curr Top Microbiol Immunol 301:203-25. 2006
  4. ncbi Application of DNA methyltransferases in targeted DNA methylation
    Albert Jeltsch
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Appl Microbiol Biotechnol 75:1233-40. 2007
  5. ncbi On the enzymatic properties of Dnmt1: specificity, processivity, mechanism of linear diffusion and allosteric regulation of the enzyme
    Albert Jeltsch
    International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
    Epigenetics 1:63-6. 2006
  6. ncbi On the substrate specificity of DNA methyltransferases. adenine-N6 DNA methyltransferases also modify cytosine residues at position N4
    A Jeltsch
    Institut fur Biochemie, Fachbereich Biologie, Heinrich Buff Ring 58, 35392 Giessen, Germany
    J Biol Chem 274:19538-44. 1999
  7. pmc Changing the target base specificity of the EcoRV DNA methyltransferase by rational de novo protein-design
    M Roth
    , Fachbereich 8, , Heinrich-Buff-Ring 58, 35392 Giessen, Germany
    Nucleic Acids Res 29:3137-44. 2001
  8. ncbi Enzymatic properties of recombinant Dnmt3a DNA methyltransferase from mouse: the enzyme modifies DNA in a non-processive manner and also methylates non-CpG [correction of non-CpA] sites
    H Gowher
    Institut für Biochemie Fachbereich 8, Justus Liebig Universitat, Heinrich Buff Ring 58, Giessen, 35392, Germany
    J Mol Biol 309:1201-8. 2001
  9. ncbi Mutational analysis of target base flipping by the EcoRV adenine-N6 DNA methyltransferase
    A Jeltsch
    Institut fur Biochemie, Fachbereich Biologie, Heinrich Buff Ring 58, Giessen, 35392, Germany
    J Mol Biol 285:1121-30. 1999
  10. ncbi The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA
    M Fatemi
    Institut für Biochemie Fachbereich 8, Justus Liebig Universitat, Heinrich Buff Ring 58, Giessen, 35392, Germany
    J Mol Biol 309:1189-99. 2001

Collaborators

  • T Friedrich
  • Michal Minczuk
  • Christian Rohde
  • Yingying Zhang
  • Tomasz P Jurkowski
  • Xiaodong Cheng
  • F Lyko
  • Stephen E Halford
  • ASHOK BHAGWAT
  • ARTHUR RIGGS
  • ERIC SELKER
  • Noreen E Murray
  • D T Dryden
  • Shuang yong Xu
  • R J Roberts
  • Hiroyuki Sasaki
  • Jurate Bitinaite
  • Humaira Gowher
  • Philipp Rathert
  • Andrea Hermann
  • Kirsten Liebert
  • Rachna Goyal
  • John R Horton
  • Vikas Handa
  • Alfred Pingoud
  • Kristin Eisenschmidt
  • Xing Zhang
  • Sabine Reither
  • Carsten Beck
  • Fuyang Li
  • Thomas Lanio
  • A Hermann
  • H Gowher
  • Mehrnaz Fatemi
  • Bernardina T F van der Gun
  • Raphaël Métivier
  • Tamas Rasko
  • Renata Z Jurkowska
  • Antal Kiss
  • Krystyna Slaska-Kiss
  • Da Jia
  • M Roth
  • Zheng Hua Xie
  • Guo Liang Xu
  • Jian ping Ding
  • Andras Simoncsits
  • Ying Zi Ge
  • Aspasia Spyridaki
  • Andreas Humeny
  • Sabine Urig
  • C Beck
  • M Fatemi
  • A Pingoud
  • Lou F M H de Leij
  • Florence Demay
  • Rene Cortese
  • Yoichi Shinkai
  • Christine Le Péron
  • Marianne G Rots
  • Christian Freund
  • Richard P Carmouche
  • Vladimir Benes
  • Elmar Weinhold
  • Frank Gannon
  • Gilles Salbert
  • Amélie Monami
  • Yasuhiko Komatsu
  • George Reid
  • Marcel H J Ruiters
  • Rozenn Gallais
  • Arunkumar Dhayalan
  • Renata Jurkowska
  • David Ibberson
  • Peter Barath
  • Marie Murakami
  • Pamela M J McLaughlin
  • Reinhold Wasserkort
  • Raluca Tamas
  • Christophe Tiffoche
  • Monika Papworth
  • Sergei Ragozin
  • Markus Roth
  • Heike Laser
  • Marcella Orwick
  • Sanjay Chahar
  • Yan Nv Huang
  • Zhao Xia Chen
  • Miklos Bekes
  • Kenichiro Hata
  • Richard Reinhardt

Detail Information

Publications53

  1. pmc Chimeric DNA methyltransferases target DNA methylation to specific DNA sequences and repress expression of target genes
    Fuyang Li
    Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    Nucleic Acids Res 35:100-12. 2007
    ..In short, we show here that it is possible to direct DNA MTase activity to predetermined sites in DNA, achieve targeted gene silencing in mammalian cell lines and interfere with HSV-1 propagation...
  2. pmc Accuracy of DNA methylation pattern preservation by the Dnmt1 methyltransferase
    Rachna Goyal
    Institut für Biochemie FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    Nucleic Acids Res 34:1182-8. 2006
    ....
  3. ncbi Molecular enzymology of mammalian DNA methyltransferases
    A Jeltsch
    School of Engineering and Science, International University Bremen, Germany
    Curr Top Microbiol Immunol 301:203-25. 2006
    ..The allosteric activation of Dnmt1 for methylation at unmodified sites is described. Wherever possible, correlations between the biochemical properties of the enzymes and their physiological functions in the cell are indicated...
  4. ncbi Application of DNA methyltransferases in targeted DNA methylation
    Albert Jeltsch
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Appl Microbiol Biotechnol 75:1233-40. 2007
    ..Thereby, gene expression can be switched off specifically, efficiently, and stably, which has a number of potential medical applications...
  5. ncbi On the enzymatic properties of Dnmt1: specificity, processivity, mechanism of linear diffusion and allosteric regulation of the enzyme
    Albert Jeltsch
    International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
    Epigenetics 1:63-6. 2006
    ..Binding of DNA to allosteric site(s) in the N-terminal part of the enzyme can lead to stimulation and inhibition of its catalytic activity depending on the nature of the substrate and effector...
  6. ncbi On the substrate specificity of DNA methyltransferases. adenine-N6 DNA methyltransferases also modify cytosine residues at position N4
    A Jeltsch
    Institut fur Biochemie, Fachbereich Biologie, Heinrich Buff Ring 58, 35392 Giessen, Germany
    J Biol Chem 274:19538-44. 1999
    ..BamHI was not detectable. On the basis of our results, we suggest that adenine-N6 and cytosine-N4 methyltransferases should be grouped into one enzyme family...
  7. pmc Changing the target base specificity of the EcoRV DNA methyltransferase by rational de novo protein-design
    M Roth
    , Fachbereich 8, , Heinrich-Buff-Ring 58, 35392 Giessen, Germany
    Nucleic Acids Res 29:3137-44. 2001
    ..Because there are no natural paragons for the variants described here, a change of the target base specificity of a DNA interacting enzyme was possible by rational de novo design of its active site...
  8. ncbi Enzymatic properties of recombinant Dnmt3a DNA methyltransferase from mouse: the enzyme modifies DNA in a non-processive manner and also methylates non-CpG [correction of non-CpA] sites
    H Gowher
    Institut für Biochemie Fachbereich 8, Justus Liebig Universitat, Heinrich Buff Ring 58, Giessen, 35392, Germany
    J Mol Biol 309:1201-8. 2001
    ....
  9. ncbi Mutational analysis of target base flipping by the EcoRV adenine-N6 DNA methyltransferase
    A Jeltsch
    Institut fur Biochemie, Fachbereich Biologie, Heinrich Buff Ring 58, Giessen, 35392, Germany
    J Mol Biol 285:1121-30. 1999
    ..The S229A variant can better flip modified bases but does not tightly lock the flipped base into the adenine-binding pocket, suggesting that Ser229 could form a contact to the flipped adenine...
  10. ncbi The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA
    M Fatemi
    Institut für Biochemie Fachbereich 8, Justus Liebig Universitat, Heinrich Buff Ring 58, Giessen, 35392, Germany
    J Mol Biol 309:1189-99. 2001
    ..It suggests that Dnmt1 might be responsible for spreading of methylation, a process that is observed during aging and carcenogenesis but may be important for de novo methylation of DNA...
  11. ncbi Engineering novel restriction endonucleases: principles and applications
    A Jeltsch
    Institut fur Biochemie, Fachbereich Biologie, Justus Liebig Universitat, Giessen, Germany
    Trends Biotechnol 14:235-8. 1996
    ....
  12. pmc Structure and function of type II restriction endonucleases
    A Pingoud
    Institut für Biochemie FB 08, Justus Liebig Universitat, Heinrich Buff Ring 58, D 35392 Giessen, Germany
    Nucleic Acids Res 29:3705-27. 2001
    ..Cleavage in the two strands usually occurs in a concerted fashion and leads to inversion of configuration at the phosphorus. The products of the reaction are DNA fragments with a 3'-OH and a 5'-phosphate...
  13. ncbi Biochemistry and biology of mammalian DNA methyltransferases
    A Hermann
    Institut fur Biochemie, FB 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392, Giessen, Germany
    Cell Mol Life Sci 61:2571-87. 2004
    ..Here we review our current understanding of the molecular enzymology of the mammalian DNA methyltransferases Dnmt1, Dnmt3a, Dnmt3b and Dnmt2 and the roles of the enzymes in the above-mentioned biological processes...
  14. ncbi How does a DNA interacting enzyme change its specificity during molecular evolution? A site-directed mutagenesis study at the DNA binding site of the DNA-(adenine-N6)-methyltransferase EcoRV
    C Beck
    Institut fur Biochemie, Fachbereich 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    Biochemistry 40:10956-65. 2001
    ..EcoRV and dam MTases. Mutagenesis at important residues within this module leads to variants that show a decreased ability to recognize the TC-part of the GATATC sequence...
  15. ncbi Circular permutations in the molecular evolution of DNA methyltransferases
    A Jeltsch
    Institut fur Biochemie, Fachbereich Biologie, Justus Liebig Universitat Giessen, Heinrich Buff Ring 58, 35392 Giessen, Germany
    J Mol Evol 49:161-4. 1999
    ..Thus, circular permutation can be regarded as a normal process in molecular evolution and a changed order of conserved amino acid motifs should not be interpreted to argue against divergent evolution...
  16. pmc Transition from nonspecific to specific DNA interactions along the substrate-recognition pathway of dam methyltransferase
    John R Horton
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Cell 121:349-61. 2005
    ..These structures illustrate the transition in enzyme-DNA interaction from nonspecific to specific interaction, suggesting that there is a temporal order for formation of specific contacts...
  17. ncbi Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L
    Humaira Gowher
    International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
    J Biol Chem 280:13341-8. 2005
    ..Following dissociation of Dnmt3L, Dnmt3A adopts a closed conformation leading to slow rates of DNA release. Therefore, Dnmt3L acts as a substrate exchange factor that accelerates DNA and AdoMet binding to de novo DNA methyltransferases...
  18. pmc Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation
    Da Jia
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322, USA
    Nature 449:248-51. 2007
    ..These results suggest a basis for the recognition and methylation of differentially methylated regions in imprinted genes, involving the detection of both nucleosome modification and CpG spacing...
  19. ncbi De novo methylation of nucleosomal DNA by the mammalian Dnmt1 and Dnmt3A DNA methyltransferases
    Humaira Gowher
    School of Engineering and Science, International University Bremen, Campus Ring 1, D 28759 Bremen, Germany
    Biochemistry 44:9899-904. 2005
    ....
  20. ncbi The Dnmt1 DNA-(cytosine-C5)-methyltransferase methylates DNA processively with high preference for hemimethylated target sites
    Andrea Hermann
    Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    J Biol Chem 279:48350-9. 2004
    ....
  21. ncbi Reversible inactivation of the CG specific SssI DNA (cytosine-C5)-methyltransferase with a photocleavable protecting group
    Philipp Rathert
    Biochemistry Laboratory International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
    Chembiochem 8:202-7. 2007
    ..Irradiation of the inactivated enzyme with near-ultraviolet light (320-400 nm) restored 60 % of the catalytic activity. This indicates that caging by DMNBB can be used for the reversible inactivation of M.SssI...
  22. ncbi Two alternative conformations of S-adenosyl-L-homocysteine bound to Escherichia coli DNA adenine methyltransferase and the implication of conformational changes in regulating the catalytic cycle
    Kirsten Liebert
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, 28759 Bremen, Germany
    J Biol Chem 282:22848-55. 2007
    ..This suggests that the hexapeptide couples specific DNA binding (Lys(9)), AdoMet binding (Trp(10)), and insertion of the flipped target base into the active site pocket (Lys(14))...
  23. ncbi Catalytic mechanism of DNA-(cytosine-C5)-methyltransferases revisited: covalent intermediate formation is not essential for methyl group transfer by the murine Dnmt3a enzyme
    Sabine Reither
    Institut fur Biochemie, FB 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392, Giessen, Germany
    J Mol Biol 329:675-84. 2003
    ..We propose that correct positioning of the flipped base and the cofactor and binding to the transition state of methyl group transfer are the most important roles of the Dnmt3a enzyme in the catalytic cycle of methyl group transfer...
  24. ncbi Methylation sensitivity of restriction enzymes interacting with GATC sites
    Andrea Hermann
    Justus Liebig Universitat Giessen, Germany
    Biotechniques 34:924-6, 928, 930. 2003
  25. ncbi A fluorimetric assay for on-line detection of DNA cleavage by restriction endonucleases
    Kristin Eisenschmidt
    Justus Liebig Universitat, Institut für Biochemie FB08, 35392 Giessen, Germany
    J Biotechnol 96:185-91. 2002
    ..This assay can be carried out in a microplate format, which allows for the analysis of many restriction endonuclease variants in parallel...
  26. ncbi The Escherichia coli dam DNA methyltransferase modifies DNA in a highly processive reaction
    Sabine Urig
    Institut fur Biochemie, Fachbereich Biologie, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    J Mol Biol 319:1085-96. 2002
    ....
  27. ncbi German human methylome project started
    Albert Jeltsch
    Cancer Res 66:7378. 2006
  28. doi Reading and writing DNA methylation
    Albert Jeltsch
    Nat Struct Mol Biol 15:1003-4. 2008
  29. pmc Protein lysine methyltransferase G9a acts on non-histone targets
    Philipp Rathert
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Nat Chem Biol 4:344-6. 2008
    ..We demonstrate potential downstream signaling pathways for methylation of non-histone proteins...
  30. doi Persistent downregulation of the pancarcinoma-associated epithelial cell adhesion molecule via active intranuclear methylation
    Bernardina T F van der Gun
    Department of Pathology and Laboratory Medicine, Section Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands
    Int J Cancer 123:484-9. 2008
    ..This is the first study showing that active DNA methylation leads to sustained silencing of endogenous EpCAM expression...
  31. doi Cyclical DNA methylation of a transcriptionally active promoter
    Raphaël Métivier
    Universite de Rennes I, CNRS, UMR 6026 Equipe SPARTE, IFR 140 GFAS, Campus de Beaulieu, 35042 Rennes Cedex, France
    Nature 452:45-50. 2008
    ..Cyclical changes in the methylation status of promoter CpGs may thus represent a critical event in transcriptional achievement...
  32. pmc Bisulfite sequencing Data Presentation and Compilation (BDPC) web server--a useful tool for DNA methylation analysis
    Christian Rohde
    School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28725 Bremen, Germany
    Nucleic Acids Res 36:e34. 2008
    ..v) A condensed file, containing all primary data in simplified format for further downstream data analysis and (vi) a custom track file for display of the results in the UCSC genome browser...
  33. ncbi Detection and quantitation of the activity of DNA methyltransferases using a biotin/avidin microplate assay
    Kirsten Liebert
    School of Engineering and Science, International University Bremen, Bremen, Germany
    Methods Mol Biol 418:149-56. 2008
    ..Furthermore, the assay is very convenient, fast and well suited for automation...
  34. ncbi Phosphorylation of serine-515 activates the Mammalian maintenance methyltransferase Dnmt1
    Rachna Goyal
    Institut fur Biochemie, Justus Liebig Universitat, Giessen, Germany
    Epigenetics 2:155-60. 2007
    ..We conclude that phosphorylation of Dnmt1 at Ser515 could be an important regulator of Dnmt1 activity during cell cycle and after proliferative stimuli...
  35. pmc Analysis of the substrate specificity of the Dim-5 histone lysine methyltransferase using peptide arrays
    Philipp Rathert
    Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Chem Biol 15:5-11. 2008
    ..Comparative analyses of peptide arrays with wild-type and mutant enzymes, therefore, are well suited to investigate the target specificity of protein methyltransferases and study epigenetic crosstalk...
  36. ncbi The M.EcoRV DNA-(adenine N6)-methyltransferase uses DNA bending for recognition of an expanded EcoDam recognition site
    Tomasz P Jurkowski
    Biochemistry Laboratory, BioRec Program, School of Engineering and Science, Jacobs University Bremen, Germany
    J Biol Chem 282:36942-52. 2007
    ..These results suggest a temporal order of the formation of protein-DNA contacts in which the Gua6-Arg128 contact forms early followed by DNA bending and, finally, the formation of the Lys11-Gua1 contact...
  37. ncbi Two substrates are better than one: dual specificities for Dnmt2 methyltransferases
    Albert Jeltsch
    School of Engineering and Science, International University Bremen, Campus Ring 1, 28759 Bremen, Germany
    Trends Biochem Sci 31:306-8. 2006
    ..This finding has important implications for understanding the evolutionary relationships among these enzymes...
  38. ncbi Mutations in DNA methyltransferase DNMT3B in ICF syndrome affect its regulation by DNMT3L
    Zheng Hua Xie
    The State Key Laboratory of Molecular Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
    Hum Mol Genet 15:1375-85. 2006
    ....
  39. pmc Specificity of DNA triple helix formation analyzed by a FRET assay
    Sabine Reither
    Institut fur Biochemie, FB8 Justus Liebig Universität, Heinrich Buff Ring 58, 35392, Giessen, Germany
    BMC Biochem 3:27. 2002
    ..A third DNA strand can bind into the major groove of a homopurine duplex DNA to form a DNA triple helix. Sequence specific triplex formation can be applied for gene targeting, gene silencing and mutagenesis...
  40. ncbi Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA
    Mehrnaz Fatemi
    Institut fur Biochemie, Justus Liebig Universitat, Giessen, Germany
    Eur J Biochem 269:4981-4. 2002
    ..This model agrees with the biochemical properties of these enzymes and provides a mechanistic basis for the functional cooperation of different DNA MTases in de novo methylation of DNA that has also been observed in vivo...
  41. ncbi Probing the DNA interface of the EcoRV DNA-(adenine-N6)-methyltransferase by site-directed mutagenesis, fluorescence spectroscopy, and UV cross-linking
    Carsten Beck
    Institut fur Biochemie, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    Biochemistry 41:14103-10. 2002
    ..Whether the observation that evolutionarily conserved contacts are formed early during complex formation is a general rule for DNA interacting enzymes or proteins that change their specificity during evolution remains to be seen...
  42. ncbi Detection and analysis of enzymatic DNA methylation of oligonucleotide substrates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    Andreas Humeny
    Institut fur Biochemie, Emil Fischer Zentrum, Friedrich Alexander Universitat Erlangen Nurnberg, Fahrstrasse 17, 91054 Erlangen, Germany
    Anal Biochem 313:160-6. 2003
    ..We provide evidence that the dam MTase modifies DNA in a processive reaction, confirming earlier findings...
  43. pmc A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
    Richard J Roberts
    New England Biolabs, Beverly, MA 01915, USA
    Nucleic Acids Res 31:1805-12. 2003
    ..It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes...
  44. ncbi The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity
    Andrea Hermann
    Institut fur Biochemie, FB 8, Heinrich Buff Ring 58, Justus Liebig Universitat, 35392 Giessen, Germany
    J Biol Chem 278:31717-21. 2003
    ..Methylation was observed at CG sites in a loose ttnCGga(g/a) consensus sequence, suggesting that Dnmt2 has a more specialized role than other mammalian DNA methyltransferases...
  45. ncbi Maintenance of species identity and controlling speciation of bacteria: a new function for restriction/modification systems?
    Albert Jeltsch
    Institut fur Biochemie, FB 08, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    Gene 317:13-6. 2003
    ....
  46. ncbi Chromatin targeting of de novo DNA methyltransferases by the PWWP domain
    Ying Zi Ge
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China
    J Biol Chem 279:25447-54. 2004
    ..Our data establish the PWWP domain as a novel chromatin/chromosome-targeting module and suggest that the PWWP-mediated chromatin association is essential for the function of the de novo methyltransferases during development...
  47. ncbi Anomalous mobility of polymerase chain reaction products after bisulfite treatment of DNA
    Vikas Handa
    Institut fur Biochemie, FB 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    Anal Biochem 333:196-8. 2004
  48. ncbi Mechanism of inhibition of DNA methyltransferases by cytidine analogs in cancer therapy
    Humaira Gowher
    School of Engineering and Science, International University Bremen, Bremen 28759, Germany
    Cancer Biol Ther 3:1062-8. 2004
    ..This review provides an insight to how the chemistry of DNA methylation is involved in the performance of these drugs targeted against it...
  49. ncbi Profound flanking sequence preference of Dnmt3a and Dnmt3b mammalian DNA methyltransferases shape the human epigenome
    Vikas Handa
    Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
    J Mol Biol 348:1103-12. 2005
    ..Furthermore, similar flanking sequence preferences have been found for the stimulation of the immune system by unmethylated CGs, suggesting a co-evolution of DNA MTases and the immune system...
  50. pmc Developing a programmed restriction endonuclease for highly specific DNA cleavage
    Kristin Eisenschmidt
    Institut fur Biochemie, Justus Liebig Universitat, Heinrich Buff Ring 58, D 35392 Giessen, Germany
    Nucleic Acids Res 33:7039-47. 2005
    ..Single base pair substitutions in the TFS prevent addressed DNA cleavage by scPvuII-TFO...
  51. ncbi Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuII
    Aspasia Spyridaki
    Department of Biology, IMBB FORTH, University of Crete, PO Box 1527, Heraklion, GR 71110, Crete, Greece
    J Mol Biol 331:395-406. 2003
    ..These results, for the first time, shed light on the pathway by which metal ions as essential cofactors enter the catalytic center of restriction endonucleases...
  52. pmc Structure and substrate recognition of the Escherichia coli DNA adenine methyltransferase
    John R Horton
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    J Mol Biol 358:559-70. 2006
    ..3) The orphaned Thy residue displays structural flexibility by adopting an extrahelical or intrahelical position where it is in contact to N120...
  53. ncbi Beyond Watson and Crick: DNA methylation and molecular enzymology of DNA methyltransferases
    Albert Jeltsch
    Institut fur Biochemie, FB 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
    Chembiochem 3:274-93. 2002
    ..In addition, the structures and mechanisms of the DNA methyltransferases, which enable them to specifically recognize their DNA targets and to induce such large conformational changes of the DNA, are discussed...