A JeltschSummaryAffiliation: International University Bremen Country: Germany Publications
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Molecular enzymology of mammalian DNA methyltransferasesA Jeltsch
School of Engineering and Science, International University Bremen, Germany
Curr Top Microbiol Immunol 301:203-25. 2006..The allosteric activation of Dnmt1 for methylation at unmodified sites is described. Wherever possible, correlations between the biochemical properties of the enzymes and their physiological functions in the cell are indicated...- On the enzymatic properties of Dnmt1: specificity, processivity, mechanism of linear diffusion and allosteric regulation of the enzymeAlbert Jeltsch
International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
Epigenetics 1:63-6. 2006..Binding of DNA to allosteric site(s) in the N-terminal part of the enzyme can lead to stimulation and inhibition of its catalytic activity depending on the nature of the substrate and effector... - Application of DNA methyltransferases in targeted DNA methylationAlbert Jeltsch
Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
Appl Microbiol Biotechnol 75:1233-40. 2007..Thereby, gene expression can be switched off specifically, efficiently, and stably, which has a number of potential medical applications... 
Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylationDa Jia
Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, Georgia 30322, USA
Nature 449:248-51. 2007..These results suggest a basis for the recognition and methylation of differentially methylated regions in imprinted genes, involving the detection of both nucleosome modification and CpG spacing...- The Dnmt1 DNA-(cytosine-C5)-methyltransferase methylates DNA processively with high preference for hemimethylated target sitesAndrea Hermann
Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
J Biol Chem 279:48350-9. 2004.... - Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3LHumaira Gowher
International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
J Biol Chem 280:13341-8. 2005..Following dissociation of Dnmt3L, Dnmt3A adopts a closed conformation leading to slow rates of DNA release. Therefore, Dnmt3L acts as a substrate exchange factor that accelerates DNA and AdoMet binding to de novo DNA methyltransferases... - Transition from nonspecific to specific DNA interactions along the substrate-recognition pathway of dam methyltransferaseJohn R Horton
Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
Cell 121:349-61. 2005..These structures illustrate the transition in enzyme-DNA interaction from nonspecific to specific interaction, suggesting that there is a temporal order for formation of specific contacts... - Catalytic mechanism of DNA-(cytosine-C5)-methyltransferases revisited: covalent intermediate formation is not essential for methyl group transfer by the murine Dnmt3a enzymeSabine Reither
Institut fur Biochemie, FB 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392, Giessen, Germany
J Mol Biol 329:675-84. 2003..We propose that correct positioning of the flipped base and the cofactor and binding to the transition state of methyl group transfer are the most important roles of the Dnmt3a enzyme in the catalytic cycle of methyl group transfer... - Methylation sensitivity of restriction enzymes interacting with GATC sitesAndrea Hermann
, Germany
Biotechniques 34:924-6, 928, 930. 2003 - De novo methylation of nucleosomal DNA by the mammalian Dnmt1 and Dnmt3A DNA methyltransferasesHumaira Gowher
School of Engineering and Science, International University Bremen, Campus Ring 1, D 28759 Bremen, Germany
Biochemistry 44:9899-904. 2005.... - Reversible inactivation of the CG specific SssI DNA (cytosine-C5)-methyltransferase with a photocleavable protecting groupPhilipp Rathert
Biochemistry Laboratory International University Bremen, School of Engineering and Science, Campus Ring 1, 28759 Bremen, Germany
Chembiochem 8:202-7. 2007..Irradiation of the inactivated enzyme with near-ultraviolet light (320-400 nm) restored 60 % of the catalytic activity. This indicates that caging by DMNBB can be used for the reversible inactivation of M.SssI... - Chimeric DNA methyltransferases target DNA methylation to specific DNA sequences and repress expression of target genesFuyang Li
Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
Nucleic Acids Res 35:100-12. 2007..In short, we show here that it is possible to direct DNA MTase activity to predetermined sites in DNA, achieve targeted gene silencing in mammalian cell lines and interfere with HSV-1 propagation... - The Escherichia coli dam DNA methyltransferase modifies DNA in a highly processive reactionSabine Urig
Institut fur Biochemie, Fachbereich Biologie, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
J Mol Biol 319:1085-96. 2002.... - A fluorimetric assay for on-line detection of DNA cleavage by restriction endonucleasesKristin Eisenschmidt
, , 35392 Giessen, Germany
J Biotechnol 96:185-91. 2002..This assay can be carried out in a microplate format, which allows for the analysis of many restriction endonuclease variants in parallel... - Two alternative conformations of S-adenosyl-L-homocysteine bound to Escherichia coli DNA adenine methyltransferase and the implication of conformational changes in regulating the catalytic cycleKirsten Liebert
Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, 28759 Bremen, Germany
J Biol Chem 282:22848-55. 2007..This suggests that the hexapeptide couples specific DNA binding (Lys(9)), AdoMet binding (Trp(10)), and insertion of the flipped target base into the active site pocket (Lys(14))... - German human methylome project startedAlbert Jeltsch
Cancer Res 66:7378. 2006 - Two substrates are better than one: dual specificities for Dnmt2 methyltransferasesAlbert Jeltsch
School of Engineering and Science, International University Bremen, Campus Ring 1, 28759 Bremen, Germany
Trends Biochem Sci 31:306-8. 2006..This finding has important implications for understanding the evolutionary relationships among these enzymes... - Phosphorylation of serine-515 activates the Mammalian maintenance methyltransferase Dnmt1Rachna Goyal
Institut fur Biochemie, Justus Liebig Universitat, Giessen, Germany
Epigenetics 2:155-60. 2007..We conclude that phosphorylation of Dnmt1 at Ser515 could be an important regulator of Dnmt1 activity during cell cycle and after proliferative stimuli... - The M.EcoRV DNA-(adenine N6)-methyltransferase uses DNA bending for recognition of an expanded EcoDam recognition siteTomasz P Jurkowski
Biochemistry Laboratory, BioRec Program, School of Engineering and Science, Jacobs University Bremen, Germany
J Biol Chem 282:36942-52. 2007..These results suggest a temporal order of the formation of protein-DNA contacts in which the Gua6-Arg128 contact forms early followed by DNA bending and, finally, the formation of the Lys11-Gua1 contact... - Analysis of the substrate specificity of the Dim-5 histone lysine methyltransferase using peptide arraysPhilipp Rathert
Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
Chem Biol 15:5-11. 2008..Comparative analyses of peptide arrays with wild-type and mutant enzymes, therefore, are well suited to investigate the target specificity of protein methyltransferases and study epigenetic crosstalk... - Detection and quantitation of the activity of DNA methyltransferases using a biotin/avidin microplate assayKirsten Liebert
School of Engineering and Science, International University Bremen, Bremen, Germany
Methods Mol Biol 418:149-56. 2008..Furthermore, the assay is very convenient, fast and well suited for automation... - Bisulfite sequencing Data Presentation and Compilation (BDPC) web server--a useful tool for DNA methylation analysisChristian Rohde
School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28725 Bremen, Germany
Nucleic Acids Res 36:e34. 2008..v) A condensed file, containing all primary data in simplified format for further downstream data analysis and (vi) a custom track file for display of the results in the UCSC genome browser... 
Cyclical DNA methylation of a transcriptionally active promoterRaphaël Métivier
Universite de Rennes I, CNRS, UMR 6026 Equipe SPARTE, IFR 140 GFAS, Campus de Beaulieu, 35042 Rennes Cedex, France
Nature 452:45-50. 2008..Cyclical changes in the methylation status of promoter CpGs may thus represent a critical event in transcriptional achievement...- Persistent downregulation of the pancarcinoma-associated epithelial cell adhesion molecule via active intranuclear methylationBernardina T F van der Gun
Department of Pathology and Laboratory Medicine, Section Medical Biology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands
Int J Cancer 123:484-9. 2008..This is the first study showing that active DNA methylation leads to sustained silencing of endogenous EpCAM expression... - Protein lysine methyltransferase G9a acts on non-histone targetsPhilipp Rathert
Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany
Nat Chem Biol 4:344-6. 2008..We demonstrate potential downstream signaling pathways for methylation of non-histone proteins... - Reading and writing DNA methylationAlbert Jeltsch
Nat Struct Mol Biol 15:1003-4. 2008 - Mutations in DNA methyltransferase DNMT3B in ICF syndrome affect its regulation by DNMT3LZheng Hua Xie
The State Key Laboratory of Molecular Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
Hum Mol Genet 15:1375-85. 2006.... - Specificity of DNA triple helix formation analyzed by a FRET assaySabine Reither
Institut fur Biochemie, FB8 Justus Liebig Universität, Heinrich Buff Ring 58, 35392, Giessen, Germany
BMC Biochem 3:27. 2002..A third DNA strand can bind into the major groove of a homopurine duplex DNA to form a DNA triple helix. Sequence specific triplex formation can be applied for gene targeting, gene silencing and mutagenesis... - Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNAMehrnaz Fatemi
Institut fur Biochemie, Justus Liebig Universitat, Giessen, Germany
Eur J Biochem 269:4981-4. 2002..This model agrees with the biochemical properties of these enzymes and provides a mechanistic basis for the functional cooperation of different DNA MTases in de novo methylation of DNA that has also been observed in vivo... - Probing the DNA interface of the EcoRV DNA-(adenine-N6)-methyltransferase by site-directed mutagenesis, fluorescence spectroscopy, and UV cross-linkingCarsten Beck
, , Heinrich-Buff-Ring 58, 35392 Giessen, Germany
Biochemistry 41:14103-10. 2002..Whether the observation that evolutionarily conserved contacts are formed early during complex formation is a general rule for DNA interacting enzymes or proteins that change their specificity during evolution remains to be seen... - Detection and analysis of enzymatic DNA methylation of oligonucleotide substrates by matrix-assisted laser desorption ionization time-of-flight mass spectrometryAndreas Humeny
, Emil-Fischer-Zentrum, , Fahrstrasse 17, 91054 Erlangen, Germany
Anal Biochem 313:160-6. 2003..We provide evidence that the dam MTase modifies DNA in a processive reaction, confirming earlier findings... - A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genesRichard J Roberts
New England Biolabs, Beverly, MA 01915, USA
Nucleic Acids Res 31:1805-12. 2003..It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes... - The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activityAndrea Hermann
Institut fur Biochemie, FB 8, Heinrich Buff Ring 58, Justus Liebig Universitat, 35392 Giessen, Germany
J Biol Chem 278:31717-21. 2003..Methylation was observed at CG sites in a loose ttnCGga(g/a) consensus sequence, suggesting that Dnmt2 has a more specialized role than other mammalian DNA methyltransferases... - Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuIIAspasia Spyridaki
Department of Biology, IMBB FORTH, University of Crete, PO Box 1527, Heraklion, GR 71110, Crete, Greece
J Mol Biol 331:395-406. 2003..These results, for the first time, shed light on the pathway by which metal ions as essential cofactors enter the catalytic center of restriction endonucleases... - Maintenance of species identity and controlling speciation of bacteria: a new function for restriction/modification systems?Albert Jeltsch
Institut fur Biochemie, FB 08, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
Gene 317:13-6. 2003.... - Chromatin targeting of de novo DNA methyltransferases by the PWWP domainYing Zi Ge
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China
J Biol Chem 279:25447-54. 2004..Our data establish the PWWP domain as a novel chromatin/chromosome-targeting module and suggest that the PWWP-mediated chromatin association is essential for the function of the de novo methyltransferases during development... - Anomalous mobility of polymerase chain reaction products after bisulfite treatment of DNAVikas Handa
, FB 8, , Heinrich-Buff-Ring 58, 35392 Giessen, Germany
Anal Biochem 333:196-8. 2004 - Mechanism of inhibition of DNA methyltransferases by cytidine analogs in cancer therapyHumaira Gowher
School of Engineering and Science, International University Bremen, Bremen 28759, Germany
Cancer Biol Ther 3:1062-8. 2004..This review provides an insight to how the chemistry of DNA methylation is involved in the performance of these drugs targeted against it... - Profound flanking sequence preference of Dnmt3a and Dnmt3b mammalian DNA methyltransferases shape the human epigenomeVikas Handa
Institut fur Biochemie, FB 08, Heinrich Buff Ring 58, Justus Liebig Universitat Giessen, 35392 Giessen, Germany
J Mol Biol 348:1103-12. 2005..Furthermore, similar flanking sequence preferences have been found for the stimulation of the immune system by unmethylated CGs, suggesting a co-evolution of DNA MTases and the immune system... - Developing a programmed restriction endonuclease for highly specific DNA cleavageKristin Eisenschmidt
, , Heinrich-Buff-Ring 58, D-35392 Giessen, Germany
Nucleic Acids Res 33:7039-47. 2005..Single base pair substitutions in the TFS prevent addressed DNA cleavage by scPvuII-TFO... - Accuracy of DNA methylation pattern preservation by the Dnmt1 methyltransferaseRachna Goyal
, Heinrich-Buff-Ring 58, , 35392 Giessen, Germany
Nucleic Acids Res 34:1182-8. 2006.... - Structure and substrate recognition of the Escherichia coli DNA adenine methyltransferaseJohn R Horton
Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
J Mol Biol 358:559-70. 2006..3) The orphaned Thy residue displays structural flexibility by adopting an extrahelical or intrahelical position where it is in contact to N120... - Beyond Watson and Crick: DNA methylation and molecular enzymology of DNA methyltransferasesAlbert Jeltsch
Institut fur Biochemie, FB 8, Justus Liebig Universitat, Heinrich Buff Ring 58, 35392 Giessen, Germany
Chembiochem 3:274-93. 2002..In addition, the structures and mechanisms of the DNA methyltransferases, which enable them to specifically recognize their DNA targets and to induce such large conformational changes of the DNA, are discussed...