M Pfaffl

Summary

Affiliation: Institute of Physiology
Country: Germany

Publications

  1. ncbi request reprint Quantification of insulin-like growth factor-1 (IGF-1) mRNA: development and validation of an internally standardised competitive reverse transcription-polymerase chain reaction
    M Pfaffl
    Institut fur Physiologie, Forschungszentrum fur Milch und Lebensmittel Weihenstephan, Technische Universitat Munchen, Freising Weihenstephan, Germany
    Exp Clin Endocrinol Diabetes 106:506-13. 1998
  2. ncbi request reprint Quantification of insulin-like growth factor-1 (IGF-1) mRNA: modulation of growth intensity by feeding results in inter- and intra-tissue-specific differences of IGF-1 mRNA expression in steers
    M Pfaffl
    Institut fur Physiologie, Forschungszentrum fur Milch und Lebensmittel Weihenstephan, Germany
    Exp Clin Endocrinol Diabetes 106:514-21. 1998
  3. pmc A new mathematical model for relative quantification in real-time RT-PCR
    M W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 29:e45. 2001
  4. ncbi request reprint Tissue-specific expression pattern of estrogen receptors (ER): quantification of ER alpha and ER beta mRNA with real-time RT-PCR
    M W Pfaffl
    Institute of Physiology, Research Centre for Milk and Food Weihenstephan, Technical University of Munich, Freising Weihenstephan, Germany
    APMIS 109:345-55. 2001
  5. ncbi request reprint Effects of synthetic progestagens on the mRNA expression of androgen receptor, progesterone receptor, oestrogen receptor alpha and beta, insulin-like growth factor-1 (IGF-1) and IGF-1 receptor in heifer tissues
    M W Pfaffl
    Institute of Physiology, Centre of Life and Food Sciences, Technical University of Munich, Germany
    J Vet Med A Physiol Pathol Clin Med 49:57-64. 2002
  6. ncbi request reprint Expression of estrogen and androgen receptor in the bovine gastrointestinal tract
    H Sauerwein
    Institut fur Physiologie, Forschungszentrum fur Milch und Lebensmittel Weihenstephan, Technische Universitat Munchen
    Dtsch Tierarztl Wochenschr 102:164-8. 1995
  7. ncbi request reprint Influence of oral application of mycophenolic acid on the clinical health status of sheep
    A I Mohr
    Institute of Animal Hygiene, Centre of Life and Food Sciences, Technical University Munich, Weihenstephaner Berg 3, D 85354 Freising, Germany
    J Vet Med A Physiol Pathol Clin Med 54:76-81. 2007
  8. ncbi request reprint Short-term effects on pro-inflammatory cytokine, lactoferrin and CD14 mRNA expression levels in bovine immunoseparated milk and blood cells treated by LPS
    C Prgomet
    Physiology Weihenstephan, Center of Life and Food Science, Technische Universitat Munchen, Weihenstephaner Berg 3, 85354 Freising Weihenstephan, Germany
    J Vet Med A Physiol Pathol Clin Med 52:317-24. 2005
  9. ncbi request reprint The mRNA expression of the members of the IGF-system in bovine corpus luteum during induced luteolysis
    T P Neuvians
    Institute of Physiology, Technical University Munich, Weihenstephaner Berg 3, Freising Weihenstephan D 85350, Germany
    Domest Anim Endocrinol 25:359-72. 2003
  10. ncbi request reprint Abundance of message for insulin-like growth factors-I and -II and for receptors for growth hormone, insulin-like growth factors-I and -II, and insulin in the intestine and liver of pre- and full-term calves
    T M Georgieva
    Division of Nutrition and Physiology, Institute of Animal Genetics, Nutrition and Housing, Faculty of Veterinary Medicine, University of Berne, CH 3012 Berne, Switzerland
    J Anim Sci 81:2294-300. 2003

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Quantification of insulin-like growth factor-1 (IGF-1) mRNA: development and validation of an internally standardised competitive reverse transcription-polymerase chain reaction
    M Pfaffl
    Institut fur Physiologie, Forschungszentrum fur Milch und Lebensmittel Weihenstephan, Technische Universitat Munchen, Freising Weihenstephan, Germany
    Exp Clin Endocrinol Diabetes 106:506-13. 1998
    ..In bulls, the IGF-1 mRNA expression was higher in m. splenius (p < 0.01) than m. gastrocnemius, indicating that locally produced IGF-1 might be important for sexually dimorphic muscle growth patterns...
  2. ncbi request reprint Quantification of insulin-like growth factor-1 (IGF-1) mRNA: modulation of growth intensity by feeding results in inter- and intra-tissue-specific differences of IGF-1 mRNA expression in steers
    M Pfaffl
    Institut fur Physiologie, Forschungszentrum fur Milch und Lebensmittel Weihenstephan, Germany
    Exp Clin Endocrinol Diabetes 106:514-21. 1998
    ..Our findings demonstrate that IGF-1 mRNA expression is regulated tissue specifically not only between different organs but also within musculature...
  3. pmc A new mathematical model for relative quantification in real-time RT-PCR
    M W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 29:e45. 2001
    ..High accuracy and reproducibility (<2.5% variation) were reached in LightCycler PCR using the established mathematical model...
  4. ncbi request reprint Tissue-specific expression pattern of estrogen receptors (ER): quantification of ER alpha and ER beta mRNA with real-time RT-PCR
    M W Pfaffl
    Institute of Physiology, Research Centre for Milk and Food Weihenstephan, Technical University of Munich, Freising Weihenstephan, Germany
    APMIS 109:345-55. 2001
    ..001) and kidney medulla (p<0.05). These data support the hypothesis that ER beta may have different biological functions than ER alpha, especially in kidney and jejunum...
  5. ncbi request reprint Effects of synthetic progestagens on the mRNA expression of androgen receptor, progesterone receptor, oestrogen receptor alpha and beta, insulin-like growth factor-1 (IGF-1) and IGF-1 receptor in heifer tissues
    M W Pfaffl
    Institute of Physiology, Centre of Life and Food Sciences, Technical University of Munich, Germany
    J Vet Med A Physiol Pathol Clin Med 49:57-64. 2002
    ..ERalpha in liver and neck muscle showed a trend of increasing expression...
  6. ncbi request reprint Expression of estrogen and androgen receptor in the bovine gastrointestinal tract
    H Sauerwein
    Institut fur Physiologie, Forschungszentrum fur Milch und Lebensmittel Weihenstephan, Technische Universitat Munchen
    Dtsch Tierarztl Wochenschr 102:164-8. 1995
    ..AR mRNA expression was not determined in the forestomaches, but was present in all intestinal segments investigated.(ABSTRACT TRUNCATED AT 250 WORDS)..
  7. ncbi request reprint Influence of oral application of mycophenolic acid on the clinical health status of sheep
    A I Mohr
    Institute of Animal Hygiene, Centre of Life and Food Sciences, Technical University Munich, Weihenstephaner Berg 3, D 85354 Freising, Germany
    J Vet Med A Physiol Pathol Clin Med 54:76-81. 2007
    ..The results of the study indicate that MPA concentrations occurring naturally in silage have no obvious impact on sheep health...
  8. ncbi request reprint Short-term effects on pro-inflammatory cytokine, lactoferrin and CD14 mRNA expression levels in bovine immunoseparated milk and blood cells treated by LPS
    C Prgomet
    Physiology Weihenstephan, Center of Life and Food Science, Technische Universitat Munchen, Weihenstephaner Berg 3, 85354 Freising Weihenstephan, Germany
    J Vet Med A Physiol Pathol Clin Med 52:317-24. 2005
    ..The constitutive transcription of CD14 mRNA in WBC and monocytes was found to be 6 to 15 times higher than in adequate milk cells...
  9. ncbi request reprint The mRNA expression of the members of the IGF-system in bovine corpus luteum during induced luteolysis
    T P Neuvians
    Institute of Physiology, Technical University Munich, Weihenstephaner Berg 3, Freising Weihenstephan D 85350, Germany
    Domest Anim Endocrinol 25:359-72. 2003
    ..There were no significant changes in IGF I mRNA expression. In conclusion, the IGF-system with all its components seems to play an important role in the very complex process of PGF2alpha-induced luteolysis in bovine CL...
  10. ncbi request reprint Abundance of message for insulin-like growth factors-I and -II and for receptors for growth hormone, insulin-like growth factors-I and -II, and insulin in the intestine and liver of pre- and full-term calves
    T M Georgieva
    Division of Nutrition and Physiology, Institute of Animal Genetics, Nutrition and Housing, Faculty of Veterinary Medicine, University of Berne, CH 3012 Berne, Switzerland
    J Anim Sci 81:2294-300. 2003
    ..In conclusion, mRNA levels of IGF-I and IGF-II and of receptors for GH, IGF-I, IGF-II, and insulin were different at different intestinal sites and in intestine and liver and changed during the perinatal period...
  11. ncbi request reprint Expression and localisation of oestrogen and progesterone receptors in the bovine mammary gland during development, function and involution
    D Schams
    Institute of Physiology, Technical University Munich Weihenstephan, D 85350 Freising, Germany
    J Endocrinol 177:305-17. 2003
    ..In conclusion, the mRNA expression and protein data for ER and PR showed clear regulatory changes, suggesting involvement of these receptors in bovine mammary gland development and involution...
  12. ncbi request reprint Characterisation of gene expression patterns in 22RV1 cells for determination of environmental androgenic/antiandrogenic compounds
    A Hartel
    Institute of Physiology, Center of Milk and Food Research, Technical University Munich, Weihenstephaner Berg 3, 85354 Freising, Germany
    J Steroid Biochem Mol Biol 84:231-8. 2003
    ....
  13. ncbi request reprint The gastrointestinal tract as target of steroid hormone action: quantification of steroid receptor mRNA expression (AR, ERalpha, ERbeta and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR
    M W Pfaffl
    Institute of Physiology, Research Centre for Milk and Food Weihenstephan, Weihenstephaner Berg 3, Technische Universitat Munchen, D 85350, Freising, Germany
    J Steroid Biochem Mol Biol 84:159-66. 2003
    ..AR and PR seem to be not estrogen dependent...
  14. ncbi request reprint Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding proteins 1, 2 and 3 in the bovine species
    M W Pfaffl
    Institute of Physiology, Center of Life and Food Sciences, Technical University of Munich, 85354 Freising Weihenstephan, Germany
    Domest Anim Endocrinol 22:91-102. 2002
    ....
  15. ncbi request reprint [Insulin-like growth factor 1 (IGF-1) mRNA and IGF-1 protein. Expression in cells of the trabecular meshwork of the bovine eye]
    Y Cao
    Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Ophthalmologe 99:555-8. 2002
    ..The purpose of the study was to determine whether cultured bovine trabecular meshwork cells and trabecular tissue ex vivo express insulin-like growth factor-1 (IGF-1) mRNA and protein...
  16. ncbi request reprint Effect of lactoferrin on selected immune system parameters and the gastrointestinal morphology in growing calves
    C Prgomet
    Lehrstuhl für Physiologie, Technische Universität München Weihenstephan, Freising, Germany
    J Anim Physiol Anim Nutr (Berl) 91:109-19. 2007
    ..Together these findings emphasize the ability of LF to stimulate prominent immune system parameters and that it has the capacity to modulate the immune responses in a positive way...
  17. ncbi request reprint Quantitative real-time RT-PCR--a perspective
    S A Bustin
    Institute of Cellular and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London, UK
    J Mol Endocrinol 34:597-601. 2005
    ....
  18. ncbi request reprint Short-term changes of mRNA expression of various inflammatory factors and milk proteins in mammary tissue during LPS-induced mastitis
    S Schmitz
    Institute of Physiology, Technical University Munich, D 85354 Freising Weihenstephan, Germany
    Domest Anim Endocrinol 26:111-26. 2004
    ..05). In conclusion, mRNA expression of most inflammatory factors increased within hours, whereas that of most milk proteins remained unchanged...
  19. ncbi request reprint Detection and quantification of mRNA expression of alpha- and beta-adrenergic receptor subtypes in the mammary gland of dairy cows
    T Inderwies
    Institute of Physiology, Technical University Munich, D 85354 Freising Weihenstephan, Germany
    Domest Anim Endocrinol 24:123-35. 2003
    ..In conclusion, eight of nine adrenergic receptors classified to date were detected and relatively quantified in the mammary gland of dairy cows...
  20. ncbi request reprint Involvement of pro-inflammatory cytokines, mediators of inflammation, and basic fibroblast growth factor in prostaglandin F2alpha-induced luteolysis in bovine corpus luteum
    T P Neuvians
    Department of Physiology, Technical University Munich, Weihenstephaner Berg 3, D 85350 Freising Weihenstephan, Germany
    Biol Reprod 70:473-80. 2004
    ..FGF-2 may participate in the suppression of cytokine-induced iNOS mRNA expression and in the prevention of an inflammatory reaction in the surrounding tissues...
  21. ncbi request reprint Milking characteristics and their relation to adrenergic receptor mRNA expression and ligand binding in the mammary gland of dairy cows
    T Inderwies
    Institute of Physiology, Technical University Munich, D 85354, Freising Weihenstephan, Germany
    Domest Anim Endocrinol 25:275-86. 2003
    ..To influence milking characteristics, individual reactions of the cow on adrenergic stimulation have to be considered...
  22. ncbi request reprint Gene expression of immunologically important factors in blood cells, milk cells, and mammary tissue of cows
    M W Pfaffl
    Institute of Physiology, Technical University Munich, Weihenstephaner Berg 3, D 85350 Freising, Germany
    J Dairy Sci 86:538-45. 2003
    ..Generally, TNFalpha and COX-2 showed their highest expression in milk cells, 5-LO in blood cells, whereas lactoferrin was mainly expressed by the mammary tissue. COX-1 was similarly expressed in all tested samples...
  23. ncbi request reprint [Effect of transforming growth factor beta 2 on phagocytosis in cultured bovine trabecular meshwork cells]
    Y Cao
    Institut für Augenheilkunde, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China
    Ophthalmologe 100:535-8. 2003
    ..This study was performed to investigate the effect of transforming growth factor beta 2 (TGF-beta 2) on phagocytosis in bovine trabecular meshwork cells in vitro...
  24. ncbi request reprint Reference histology of veal calf genital and endocrine tissues - an update for screening on hormonal growth promoters
    M J Groot
    RIKILT Institute of Food Safety, 6700 AE Wageningen, The Netherlands
    J Vet Med A Physiol Pathol Clin Med 54:238-46. 2007
    ..This experiment showed that it is possible to raise veal calves according to the practice without getting positive histology in the prostate or Bartholin's gland...