George Iliakis

Summary

Country: Germany

Publications

  1. doi request reprint DNA double strand break repair inhibition as a cause of heat radiosensitization: re-evaluation considering backup pathways of NHEJ
    George Iliakis
    University of Duisburg Essen Medical School, Institute of Medical Radiation Biology, Essen, Germany
    Int J Hyperthermia 24:17-29. 2008
  2. doi request reprint Alternative end-joining repair pathways are the ultimate backup for abrogated classical non-homologous end-joining and homologous recombination repair: Implications for the formation of chromosome translocations
    George Iliakis
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany Electronic address
    Mutat Res Genet Toxicol Environ Mutagen 793:166-75. 2015
  3. pmc DNA double-strand break repair as determinant of cellular radiosensitivity to killing and target in radiation therapy
    Emil Mladenov
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School Essen, Germany
    Front Oncol 3:113. 2013
  4. pmc PARP-1 and Ku compete for repair of DNA double strand breaks by distinct NHEJ pathways
    Minli Wang
    University of Duisburg Essen, Medical School, Institute of Medical Radiation Biology, Hufeland Street 55, 45122 Essen, Germany
    Nucleic Acids Res 34:6170-82. 2006
  5. pmc Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells
    Hiroshi Arakawa
    Institute for Radiocytogenetics, German Research Center for Environmental Health, 85764 Neuherberg, Germany
    Nucleic Acids Res 40:2599-610. 2012
  6. ncbi request reprint The impact of serotonin on the development of bystander damage assessed by γ-H2AX foci analysis
    Holger Klammer
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Int J Radiat Biol 88:777-80. 2012
  7. pmc Processing of DNA double strand breaks by alternative non-homologous end-joining in hyperacetylated chromatin
    Vasilissa Manova
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Hufelandstr, 55, 45122, Essen, Germany
    Genome Integr 3:4. 2012
  8. doi request reprint In vitro rejoining of double strand breaks in genomic DNA
    George Iliakis
    Institute of Medical Radiation Biology, Medical School, University of Duisburg Essen, Essen, Germany
    Methods Mol Biol 920:471-84. 2012
  9. doi request reprint Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication
    George Iliakis
    Institute of Medical Radiation Biology, Medical School, University of Duisburg Essen, Essen, Germany
    Methods Mol Biol 920:591-602. 2012
  10. doi request reprint Backup pathways of NHEJ in cells of higher eukaryotes: cell cycle dependence
    George Iliakis
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Radiother Oncol 92:310-5. 2009

Collaborators

  • Satyendra K Singh
  • Ya Wang
  • Lihua Zhang
  • Mario Moscariello
  • Noritaka Adachi
  • Gisela Taucher-Scholz
  • Alexandra Gellhaus
  • Andreas Bockisch
  • Martin Stuschke
  • W J Curran
  • T K Pandita
  • Aya Kurosawa
  • Harry Scherthan
  • LAWRENCE THOMPSON
  • Zhao Chong Zeng
  • Gloria Li
  • Huichen Wang
  • Minli Wang
  • Emil Mladenov
  • Wenqi Wu
  • Holger Klammer
  • Aashish Soni
  • Simon Magin
  • Fanghua Li
  • Bustanur Rosidi
  • Hiroshi Arakawa
  • Frank Windhofer
  • Gabriel E Pantelias
  • Xiang Wang
  • Yanlei Cheng
  • Katja Paul
  • Georgia I Terzoudi
  • Janapriya Saha
  • Hongyan Wang
  • Katja Paul-Konietzko
  • Maria Siemann
  • Agnes Schipler
  • Theresa Bednar
  • Vasilissa Manova
  • Ronel Perrault
  • Christian Staudt
  • MUNIRA KADHIM
  • Xiaoxiang Fan
  • Sascha Raschke
  • Tamara Mussfeldt
  • Andrea Kinner
  • Peter Tamulevicius
  • Weizhong Wu
  • Jun Guan
  • Wilfried Böcker
  • Marta Martin
  • Ferya Banaz-Yaşar
  • Adam P Dicker
  • Girdhar G Sharma
  • Juergen Thomale
  • Maria Papaioannou
  • Tamara Murmann
  • Martha Grabos
  • Nadine Coym
  • Alena Bencsik-Theilen
  • Veronika Mladenova
  • Alena A Bencsik-Theilen
  • Nge Cheong
  • Aparna Sharma
  • Edward M Johnson
  • Jay Rappaport
  • Meijuan Wang
  • Shohreh Amini
  • Nune Darbinian-Sarkissian
  • Krzysztof Reiss
  • Kamel Khalili
  • Anna Genesca
  • Kaliopi N Manola
  • Isabel Jaco
  • Elke Winterhager
  • Rabea Tischka
  • Laura Tusell
  • Laura Latre
  • Josep Egozcue
  • Guillermo E Taccioli
  • Maria A Blasco
  • Jac A Nickoloff
  • Simon N Powell
  • Wilfried Boecker
  • Torian L Williams
  • Varsha Gandhi
  • Jerry W Shay
  • Derrick S Grant
  • Wei Qin
  • Arun Gupta

Detail Information

Publications62

  1. doi request reprint DNA double strand break repair inhibition as a cause of heat radiosensitization: re-evaluation considering backup pathways of NHEJ
    George Iliakis
    University of Duisburg Essen Medical School, Institute of Medical Radiation Biology, Essen, Germany
    Int J Hyperthermia 24:17-29. 2008
    ..We conclude with a model reclaiming a central role for DSBs in heat radiosensitization...
  2. doi request reprint Alternative end-joining repair pathways are the ultimate backup for abrogated classical non-homologous end-joining and homologous recombination repair: Implications for the formation of chromosome translocations
    George Iliakis
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany Electronic address
    Mutat Res Genet Toxicol Environ Mutagen 793:166-75. 2015
    ..We discuss possible ramifications of this model in cellular DSB management and pathway choice, and analyze its implications in radiation carcinogenesis and the design of novel therapeutic approaches. ..
  3. pmc DNA double-strand break repair as determinant of cellular radiosensitivity to killing and target in radiation therapy
    Emil Mladenov
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School Essen, Germany
    Front Oncol 3:113. 2013
    ..Finally, we discuss promising alternatives for targeting DSB repair to improve radiation therapy and cancer treatment...
  4. pmc PARP-1 and Ku compete for repair of DNA double strand breaks by distinct NHEJ pathways
    Minli Wang
    University of Duisburg Essen, Medical School, Institute of Medical Radiation Biology, Hufeland Street 55, 45122 Essen, Germany
    Nucleic Acids Res 34:6170-82. 2006
    ..This form of DSB repair is sensitive to PARP-1 inhibitors. The results define the function of PARP-1 in DSB repair and characterize a candidate pathway responsible for joining errors causing genomic instability and cancer...
  5. pmc Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells
    Hiroshi Arakawa
    Institute for Radiocytogenetics, German Research Center for Environmental Health, 85764 Neuherberg, Germany
    Nucleic Acids Res 40:2599-610. 2012
    ..Our observations demonstrate a high functional versatility for the evolutionary new LigIII in DNA replication and mitochondrial metabolism, and suggest the presence of an alternative pathway for Okazaki fragment ligation...
  6. ncbi request reprint The impact of serotonin on the development of bystander damage assessed by γ-H2AX foci analysis
    Holger Klammer
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Int J Radiat Biol 88:777-80. 2012
    ..In a recent publication, serotonin was found to have a strong modulating effect on the size of bystander effects detected using colony formation assays...
  7. pmc Processing of DNA double strand breaks by alternative non-homologous end-joining in hyperacetylated chromatin
    Vasilissa Manova
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Hufelandstr, 55, 45122, Essen, Germany
    Genome Integr 3:4. 2012
    ..abstract:..
  8. doi request reprint In vitro rejoining of double strand breaks in genomic DNA
    George Iliakis
    Institute of Medical Radiation Biology, Medical School, University of Duisburg Essen, Essen, Germany
    Methods Mol Biol 920:471-84. 2012
    ..We describe the protocols developed to carry out and analyze these in vitro reactions, including procedures for the preparation of cell extract and the preparation of the substrate DNA ("naked" DNA or nuclei)...
  9. doi request reprint Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication
    George Iliakis
    Institute of Medical Radiation Biology, Medical School, University of Duisburg Essen, Essen, Germany
    Methods Mol Biol 920:591-602. 2012
    ..Nevertheless, the fact that several steps of DNA replication initiation are carried out in this in vitro assay by TAg and not the corresponding cellular factors, might be considered as a limitation of the approach...
  10. doi request reprint Backup pathways of NHEJ in cells of higher eukaryotes: cell cycle dependence
    George Iliakis
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Radiother Oncol 92:310-5. 2009
    ..They generate a framework for investigating the mechanistic basis of HRR contribution to DSB repair...
  11. doi request reprint Widespread dependence of backup NHEJ on growth state: ramifications for the use of DNA-PK inhibitors
    Satyendra K Singh
    Institute of Medical Radiation Biology, University Duisburg Essen Medical School, Essen, Germany
    Int J Radiat Oncol Biol Phys 79:540-8. 2011
    ..Because the different D-NHEJ mutants show phenotypes distinct in their details, it is necessary to characterize the dependence of their DSB repair capacity on growth state and to explore species-specific responses...
  12. pmc Histone H1 functions as a stimulatory factor in backup pathways of NHEJ
    Bustanur Rosidi
    University of Duisburg Essen, Medical School, Institute of Medical Radiation Biology, 45122 Essen, Germany
    Nucleic Acids Res 36:1610-23. 2008
    ..Since histone H1 has been shown to counteract D-NHEJ, these observations and the known functions of the protein identify it as a putative alignment factor operating preferentially within B-NHEJ...
  13. ncbi request reprint Plasmid-based assays for DNA end-joining in vitro
    George Iliakis
    Institute of Medical Radiation Biology, Medical School, University of Duisburg Essen, Essen, Germany
    Methods Mol Biol 314:123-31. 2006
    ..The assay is powerful and easy to perform, but one should be aware that it represents an oversimplification, as it does not consider the in vivo organization of DNA into chromatin...
  14. doi request reprint Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells
    Holger Klammer
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Cancer Res 70:8498-506. 2010
    ..The results point to enhanced NHEJ as a mechanism of AR and suggest that AR may be transmitted to bystander cells through factors generating replication-mediated DSBs...
  15. ncbi request reprint Repair of radiation induced DNA double strand breaks by backup NHEJ is enhanced in G2
    Wenqi Wu
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Hufelandstr 55, Essen, Germany
    DNA Repair (Amst) 7:329-38. 2008
    ..The results show a new and potentially important cell cycle regulation of B-NHEJ and generate a framework to investigate the mechanistic basis of HRR contribution to DSB repair...
  16. ncbi request reprint Analysis of inhibition of DNA replication in irradiated cells using the SV40-based in vitro assay of DNA replication
    George Iliakis
    Institute of Medical Radiation Biology, Medical School, University of Duisburg Essen, Essen, Germany
    Methods Mol Biol 314:61-71. 2006
    ..The assay is powerful but is limited by the fact that initiation steps carried out by the TAg in vitro may have different cellular determinants...
  17. pmc Post-irradiation chemical processing of DNA damage generates double-strand breaks in cells already engaged in repair
    Satyendra K Singh
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, 45122 Essen, Germany
    Nucleic Acids Res 39:8416-29. 2011
    ..This subset of delayed DSBs may challenge DDR, may affect the perceived repair kinetics and requires further characterization...
  18. ncbi request reprint Marked dependence on growth state of backup pathways of NHEJ
    Frank Windhofer
    Institute of Medical Radiation Biology, University Duisburg Essen Medical School, Essen, Germany
    Int J Radiat Oncol Biol Phys 68:1462-70. 2007
    ..Recent evidence implicates growth signaling in the regulation of D-NHEJ. This study was intended to determine whether the ability to repair DSBs by B-NHEJ also depends on growth state...
  19. doi request reprint Lipofection and nucleofection of substrate plasmid can generate widely different readings of DNA end-joining efficiency in different cell lines
    Simon Magin
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    DNA Repair (Amst) 12:148-60. 2013
    ....
  20. doi request reprint Extensive repair of DNA double-strand breaks in cells deficient in the DNA-PK-dependent pathway of NHEJ after exclusion of heat-labile sites
    Satyendra K Singh
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Radiat Res 172:152-64. 2009
    ....
  21. ncbi request reprint In vitro rejoining of double-strand breaks in genomic DNA
    George Iliakis
    Institute of Medical Radiation Biology, Medical School, University of Duisburg Essen, Essen, Germany
    Methods Mol Biol 314:95-108. 2006
    ..Here, we describe the protocols developed to carry out and analyze these in vitro reactions, including procedures for preparation of cell extract and the preparation of the substrate DNA ("naked" DNA or nuclei)...
  22. ncbi request reprint Enhanced use of backup pathways of NHEJ in G2 in Chinese hamster mutant cells with defects in the classical pathway of NHEJ
    Wenqi Wu
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Radiat Res 170:512-20. 2008
    ....
  23. ncbi request reprint Dependence of adaptive response and its bystander transmission on the genetic background of tested cells
    Holger Klammer
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Int J Radiat Biol 88:720-6. 2012
    ..Importantly, AR was 'transmitted' to non-irradiated bystander cells through transfer of medium from cells that had received a priming dose of IR. Here, we examine the influence of the genetic background in these responses...
  24. doi request reprint Application of alkaline sucrose gradient centrifugation in the analysis of DNA replication after DNA damage
    Sascha Raschke
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Methods Mol Biol 521:329-42. 2009
    ..replicon initiation, which is essential for an in-depth analysis of the intra-S-phase checkpoint. This ability makes the technique unique and justifies its somewhat labour-intensive nature...
  25. ncbi request reprint Homology-directed repair is required for the development of radioresistance during S phase: interplay between double-strand break repair and checkpoint response
    Peter Tamulevicius
    Institute of Medical Radiation Biology, University Duisburg Essen Medical School, Essen, Germany
    Radiat Res 167:1-11. 2007
    ..An effort is made to integrate relevant phenomena of radiation action, such as low-dose radiosensitivity and the G(2) assay in this scheme...
  26. doi request reprint Inhibition of B-NHEJ in plateau-phase cells is not a direct consequence of suppressed growth factor signaling
    Satyendra K Singh
    Institute of Medical Radiation Biology, University Duisburg Essen Medical School, Essen, Germany
    Int J Radiat Oncol Biol Phys 84:e237-43. 2012
    ..Using new, standardized serum-deprivation protocols, we re-examine the growth requirements of B-NHEJ and test the role of epidermal growth factor receptor (EGFR) signaling in its regulation...
  27. pmc DNA ligases I and III cooperate in alternative non-homologous end-joining in vertebrates
    Katja Paul
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    PLoS ONE 8:e59505. 2013
    ..Together with results on DNA replication, these observations uncover a remarkable and previously unappreciated functional flexibility and interchangeability between LIG1 and LIG3...
  28. pmc Requirement for Parp-1 and DNA ligases 1 or 3 but not of Xrcc1 in chromosomal translocation formation by backup end joining
    Aashish Soni
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, 45122 Essen, Germany
    Nucleic Acids Res 42:6380-92. 2014
    ..These observations advance our understanding of chromosome aberration formation and have implications for the mechanism of action of Parp inhibitors. ..
  29. doi request reprint Effects of chromatin decondensation on alternative NHEJ
    Mario Moscariello
    Institute of Medical Radiation Biology, University of Duisburg Essen, Hufelandstrasse 55, 45122 Essen, Germany
    DNA Repair (Amst) 12:972-81. 2013
    ..The observations reported here may therefore contribute to the development of drug-based A-NHEJ suppression-strategies aiming at optimizing cancer treatment outcomes and possibly also at suppressing carcinogenesis. ..
  30. ncbi request reprint DNA damage checkpoint control in cells exposed to ionizing radiation
    George Iliakis
    Institute of Medical Radiation Biology, University of Essen Medical School, Hufelanstrasse 55, 45122 Essen, Germany
    Oncogene 22:5834-47. 2003
    ..The role of checkpoints in genomic stability and the potential of improving the treatment of cancer by DNA damage inducing agents through checkpoint abrogation are also briefly outlined...
  31. pmc Reduced contribution of thermally labile sugar lesions to DNA double strand break formation after exposure to heavy ions
    Satyendra K Singh
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Hufelandstr 55, Essen, 45122, Germany
    Radiat Oncol 8:77. 2013
    ..The consequences of these effects to the relative biological effectiveness are discussed...
  32. doi request reprint Reduced contribution of thermally-labile sugar lesions to DNA double-strand break formation after exposure to neutrons
    Satyendra K Singh
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Radiat Res 178:581-90. 2012
    ....
  33. doi request reprint Initial characterization of a low-molecular-weight factor enhancing the checkpoint response
    Xiaoxiang Fan
    University of Duisburg Essen, Medical School, Institute of Medical Radiation Biology, 45122 Essen, Germany
    Radiat Res 174:424-35. 2010
    ..Compounds with properties such as those of GAMA bridge intercellular communication with the DNA damage response and may function as radioprotectors...
  34. ncbi request reprint Low levels of DNA ligases III and IV sufficient for effective NHEJ
    Frank Windhofer
    Institute of Medical Radiation Biology, University Duisburg Essen Medical School, Essen, Germany
    J Cell Physiol 213:475-83. 2007
    ..The results suggest that complete coverage for D-NHEJ or B-NHEJ is afforded by very low ligase levels and demonstrate residual end joining by DNA ligase IV in cells of patients with mutations in LIG4...
  35. doi request reprint Inhibition of Homologous Recombination and Promotion of Mutagenic Repair of DNA Double-Strand Breaks Underpins Arabinoside-Nucleoside Analogue Radiosensitization
    Simon Magin
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Mol Cancer Ther 14:1424-33. 2015
    ....
  36. doi request reprint Role for Artemis nuclease in the repair of radiation-induced DNA double strand breaks by alternative end joining
    Mario Moscariello
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    DNA Repair (Amst) 31:29-40. 2015
    ..We conclude that Artemis is a nuclease participating in DSB repair by all major repair pathways. ..
  37. doi request reprint The yield of DNA double strand breaks determined after exclusion of those forming from heat-labile lesions predicts tumor cell radiosensitivity to killing
    Yanlei Cheng
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Germany
    Radiother Oncol 116:366-73. 2015
    ....
  38. doi request reprint Marked contribution of alternative end-joining to chromosome-translocation-formation by stochastically induced DNA double-strand-breaks in G2-phase human cells
    Aashish Soni
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Mutat Res Genet Toxicol Environ Mutagen 793:2-8. 2015
    ..We discuss DSB localization in the genome, characteristics of the DSB and the cell cycle as potential causes of the divergent results generated with IR and designer nucleases. ..
  39. pmc Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin
    Andrea Kinner
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Hufelandstrasse 55, 45122 Essen, Germany
    Nucleic Acids Res 36:5678-94. 2008
    ..We conclude with a critical analysis of the strengths and weaknesses of the approach and present some interesting applications of the resulting methodology...
  40. ncbi request reprint Computational Methods for analysis of foci: validation for radiation-induced gamma-H2AX foci in human cells
    Wilfried Böcker
    Institute of Medical Radiation Biology, University Duisburg Essen Medical School, Essen, Germany
    Radiat Res 165:113-24. 2006
    ..The computational methods presented extend to images generated by digital cameras...
  41. doi request reprint Induction and repair of DNA double strand breaks: the increasing spectrum of non-homologous end joining pathways
    Emil Mladenov
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Hufelandstr 55, 45122 Essen, Germany
    Mutat Res 711:61-72. 2011
    ..We place particular emphasis on backup pathways of NHEJ and summarize their increasing significance in various cellular processes, as well as their potential contribution to carcinogenesis...
  42. doi request reprint Bystander effects as manifestation of intercellular communication of DNA damage and of the cellular oxidative status
    Holger Klammer
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Cancer Lett 356:58-71. 2015
    ..Such responses are thought to protect the organism by enhancing repair in a community of cells and by eliminating severely damaged cells. ..
  43. doi request reprint DNA Ligases I and III Support Nucleotide Excision Repair in DT40 Cells with Similar Efficiency
    Katja Paul-Konietzko
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Photochem Photobiol 91:1173-80. 2015
    ..We show for the first time in a clean genetic background a functional redundancy in NER between Lig1 and Lig3, which appears to be cell cycle independent and which is likely to contribute to the stability of vertebrate genomes. ..
  44. doi request reprint The contribution of thermally labile sugar lesions to DNA double-strand break formation in cells grown in the presence of BrdU
    Fanghua Li
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Int J Radiat Biol 91:312-20. 2015
    ..Since BrdU radiosensitization might be associated with increased yields and reduced reparability of DSB, we investigated whether BrdU underpins these effects by shifting the balance in the generation of TLSL...
  45. pmc DNA double-strand-break complexity levels and their possible contributions to the probability for error-prone processing and repair pathway choice
    Agnes Schipler
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, 45122 Essen, Germany
    Nucleic Acids Res 41:7589-605. 2013
    ..In the same framework, we also analyze the rational of DSB repair pathway choice. ..
  46. ncbi request reprint Cell line specific modulation of connexin43 expression after exposure to ionizing radiation
    Ferya Banaz-Yaşar
    Institute of Anatomy, University Hospital Essen, Essen, Germany
    Cell Commun Adhes 12:249-59. 2005
    ..Taken together, our data show a cell line specific modulation of Cx43 expression after exposure to X-rays...
  47. pmc DNA-PKcs subunits in radiosensitization by hyperthermia on hepatocellular carcinoma hepG2 cell line
    Zhao Chong Zeng
    Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China
    World J Gastroenterol 8:797-803. 2002
    ..To investigate the role of DNA-PKcs subunits in radiosensitization by hyperthermia on hepatocellular carcinoma HepG(2) cell lines...
  48. ncbi request reprint Caffeine could not efficiently sensitize homologous recombination repair-deficient cells to ionizing radiation-induced killing
    Huichen Wang
    Department of Radiation Oncology, Kimmel Cancer Center of Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Radiat Res 159:420-5. 2003
    ..RAD51 and RAD54 are the important factors for HRR. Our results indicate that the checkpoint responses abolished by caffeine (S and G(2)) mainly affect HRR, which results in cell radiosensitization...
  49. ncbi request reprint hTERT associates with human telomeres and enhances genomic stability and DNA repair
    Girdhar G Sharma
    Radiation and Cancer Biology Division, Washington University School of Medicine, St Louis, MO 63108, USA
    Oncogene 22:131-46. 2003
    ..These novel functions of telomerase are distinct from its known effect on telomere length and have potentially important biological consequences...
  50. pmc Biochemical evidence for Ku-independent backup pathways of NHEJ
    Huichen Wang
    Department of Radiation Oncology, Division of Experimental Radiation Oncology, Kimmel Cancer Center, Jefferson Medical College, Philadelphia, PA 19107, USA
    Nucleic Acids Res 31:5377-88. 2003
    ..A chromatin-based model of DNA DSB rejoining accommodating biochemical and genetic results is presented and deviations between in vitro and in vivo results discussed...
  51. ncbi request reprint Checkpoint abrogation in G2 compromises repair of chromosomal breaks in ataxia telangiectasia cells
    Georgia I Terzoudi
    Institute of Nuclear Technology and Radiation Protection, National Centre for Scientific Research Demokritos, Aghia Paraskevi Attikis, Athens, Greece
    Cancer Res 65:11292-6. 2005
    ..Failure to arrest will lead to chromatin condensation and conversion of unrepaired DNA DSBs to chromosomal breaks during G(2)-to-M phase transition...
  52. ncbi request reprint Postreplicative joining of DNA double-strand breaks causes genomic instability in DNA-PKcs-deficient mouse embryonic fibroblasts
    Marta Martin
    Department of Cell Biology, Physiology, and Immunology, Institute of Biotechnology and Biomedicine, Universitat Autonoma de Barcelona, Bellaterra, Spain
    Cancer Res 65:10223-32. 2005
    ..The preponderance of sister chromatid fusion as a product of incorrect joining points to a possible defect in S-phase arrest and emphasizes proximity in these misrepair events...
  53. ncbi request reprint DNA ligase III as a candidate component of backup pathways of nonhomologous end joining
    Huichen Wang
    Department of Radiation Oncology, Division of Experimental Radiation Oncology, Kimmel Cancer Center, Jefferson Medical College, Philadelphia, Pennsylvania 19122, USA
    Cancer Res 65:4020-30. 2005
    ..These in vivo and in vitro observations identify DNA ligase III as a candidate component for B-NHEJ and point to additional factors contributing to NHEJ efficiency...
  54. ncbi request reprint ATR affecting cell radiosensitivity is dependent on homologous recombination repair but independent of nonhomologous end joining
    Hongyan Wang
    Department of Radiation Oncology, Kimmel Cancer Center of Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Cancer Res 64:7139-43. 2004
    ..These results indicate that the effects of ATR on cell radiosensitivity are independent of NHEJ but are linked to HRR that may be affected by the deficient S and G(2) checkpoints...
  55. ncbi request reprint Backup pathways of NHEJ are suppressed by DNA-PK
    Ronel Perrault
    Department of Radiation Oncology, Division of Experimental Radiation Oncology, Kimmel Cancer Center, Jefferson Medical College, Philadelphia, Pennsylvania 19107, USA
    J Cell Biochem 92:781-94. 2004
    ..Thus, DNA-PK suppresses alternative pathways of end joining by efficiently binding DNA ends and shunting them to D-NHEJ...
  56. ncbi request reprint Caffeine inhibits homology-directed repair of I-SceI-induced DNA double-strand breaks
    Huichen Wang
    Department of Radiation Oncology of Kimmel Cancer Center, Jefferson Medical College, Philadelphia, PA 19107, USA
    Oncogene 23:824-34. 2004
    ..In summary, our results identify inhibition of HDR as a significant contributor to caffeine radiosensitization...
  57. ncbi request reprint Evidence for the involvement of Puralpha in response to DNA replication stress
    Huichen Wang
    Department of Neuroscience, Center for Neurovirology, Temple University School of Medicine, Philadelphia, Pennsylvania 19122, USA
    Cancer Biol Ther 6:596-602. 2007
    ..These results suggest a role for Puralpha as a caretaker protein that is involved in the repair of DSBs induced by stalled replication forks...
  58. ncbi request reprint Targeting angiogenic processes by combination low-dose paclitaxel and radiation therapy
    Adam P Dicker
    Department of Radiation Oncology, Kimmel Cancer Center, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania 19107 5097, USA
    Am J Clin Oncol 26:e45-53. 2003
    ..The combination of low-dose paclitaxel and radiation suggests a complementary strategy with potential clinical ramifications to target angiogenesis-dependent malignancies...
  59. ncbi request reprint Caffeine-induced radiosensitization is independent of nonhomologous end joining of DNA double-strand breaks
    Xiang Wang
    Department of Radiation Oncology, Kimmel Cancer Center of Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Radiat Res 159:426-32. 2003
    ..Our results indicate that caffeine-induced radiosensitization occurs by affecting an NHEJ-independent process, possibly HRR...
  60. ncbi request reprint Ku affects the CHK1-dependent G(2) checkpoint after ionizing radiation
    Xiang Wang
    Department of Radiation Oncology, Kimmel Cancer Center of Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Cancer Res 62:6031-4. 2002
    ..These data indicate that the stronger G(2) checkpoint response shown in Ku80(-/-) cells is CHK1 dependent and suggest that the CHK1-dependent checkpoint response contributes to the highly efficient HRR in such cells...
  61. ncbi request reprint Premature chromosome condensation reveals DNA-PK independent pathways of chromosome break repair
    Georgia I Terzoudi
    Institute of Radioisotopes and Radiodiagnostic Products, National Centre for Scientific Research Demokritos, 15310 Ag Paraskevi Attikis, Athens, Greece
    Int J Oncol 33:871-9. 2008
    ..The understanding of chromosomal aberration formation allows mechanistic explanations for the carcinogenic potential of D-NHEJ defects...