Research Topics
Genomes and Genes | Christian M T SpahnSummaryAffiliation: Humboldt University Country: Germany Publications
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Detail Information
Publications
Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implicationsRajendra K Agrawal
Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201 0509, USA
Proc Natl Acad Sci U S A 101:8900-5. 2004....- Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocationChristian M T Spahn
Wadsworth Center, Health Research Inc, Howard Hughes Medical Institute, Albany, NY 12201 0509, USA
EMBO J 23:1008-19. 2004..sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction... - Exploring conformational modes of macromolecular assemblies by multiparticle cryo-EMChristian M T Spahn
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
Curr Opin Struct Biol 19:623-31. 2009..Their applications point to the future of cryo-EM as an experimental method uniquely suited to visualize the conformational modes of large macromolecular complexes and machines... - Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sitesAndreas H Ratje
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
Nature 468:713-6. 2010..These findings provide direct structural and mechanistic insight into the 'missing link' in terms of tRNA intermediates involved in the universally conserved translocation process... 
The complex of tmRNA-SmpB and EF-G on translocating ribosomesDavid J F Ramrath
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
Nature 485:526-9. 2012....
A new tRNA intermediate revealed on the ribosome during EF4-mediated back-translocationSean R Connell
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
Nat Struct Mol Biol 15:910-5. 2008..Our results, therefore, provide insight into the underlying structural principles governing back-translocation...- Structural basis for interaction of the ribosome with the switch regions of GTP-bound elongation factorsSean R Connell
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
Mol Cell 25:751-64. 2007..Therefore, a network of interaction with the ribosome establishes the active GTP conformation of EF-G and thus facilitates GTP hydrolysis and tRNA translocation... - Structure of the ribosome-bound cricket paralysis virus IRES RNAMartin Schuler
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
Nat Struct Mol Biol 13:1092-6. 2006..Our results exemplify the molecular strategy for recruitment of an IRES and reveal the dynamic features necessary for internal initiation... - GTPase activation of elongation factor EF-Tu by the ribosome during decodingJan Christian Schuette
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Berlin, Germany
EMBO J 28:755-65. 2009..Thus, the structure provides insights into the molecular mechanism of signalling codon recognition from the decoding centre of the 30S subunit to the GTPase centre of EF-Tu... - Localization and dynamic behavior of ribosomal protein L30eMario Halic
Institute of Biochemistry, Charite, University Medical School Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
Nat Struct Mol Biol 12:467-8. 2005..5 A. L30e is part of the interface between large and small subunits, where it dynamically participates in the formation of the two intersubunit bridges eB9 and B4... - Structure of the signal recognition particle interacting with the elongation-arrested ribosomeMario Halic
Institute of Biochemistry, Charite, University Medical School, Humboldt University of Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
Nature 427:808-14. 2004.... - Structure and dynamics of the mammalian ribosomal pretranslocation complexTatyana Budkevich
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
Mol Cell 44:214-24. 2011.... - Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factorChristian M T Spahn
Howard Hughes Medical Institute, Health Research Inc. at, Albany, NY 10012, USA
Cell 118:465-75. 2004..Conformational changes in the ribosome as well as within the IRES itself show that CrPV IRES actively manipulates the ribosome. CrPV-like IRES elements seem to act as RNA-based translation factors... - Multiparticle cryo-EM of ribosomesJustus Loerke
Institut fur Medizinische Physik und Biophysik, Charite, Universitatsmedizin Berlin, Berlin, Germany
Methods Enzymol 483:161-77. 2010..This increases information gained from single states, while exploiting the coexistence of multiple states to gather comprehensive mechanistic insight into biological processes like ribosomal translocation... - Identification of the versatile scaffold protein RACK1 on the eukaryotic ribosome by cryo-EMJayati Sengupta
Health Research, Inc, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, New York 12201-0509, USA
Nat Struct Mol Biol 11:957-62. 2004..One face of RACK1 exposes the WD-repeats as a platform for interactions with kinases and receptors. Using this platform, RACK1 can recruit other proteins to the ribosome... - Structural basis for the binding of IRES RNAs to the head of the ribosomal 40S subunitMargarita Muhs
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
Nucleic Acids Res 39:5264-75. 2011.... - A dose-rate effect in single-particle electron microscopyJames Z Chen
Howard Hughes Medical Institute, Rosenstiel Center MS029, Brandeis University, 415 South Street, Waltham, MA 02545 9110, USA
J Struct Biol 161:92-100. 2008..The improvement in image quality, especially for multiple-exposure data collection, will help single-particle cryo-EM to reach higher resolution... - An assay for local quality in cryo-electron micrographs of single particlesHaixiao Gao
Howard Hughes Medical Institute, Wadsworth Center, Albany, New York 12201-0509, USA
Ultramicroscopy 93:169-78. 2002..The method of assaying, resulting in "local quality maps", may be useful as a general tool for screening micrographs used as input for reconstructions... - Building functional modules from molecular interactionsKlaus Peter Hofmann
Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Campus Mitte, Chariteplatz 1, 10098 Berlin, Germany
Trends Biochem Sci 31:497-508. 2006..Further progress in this field, where systems biology and biochemistry meet, will depend on iterative validation of the experimental and theoretical approaches... - Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcinJoerg M Harms
Cluster of Excellence for Macromolecular Complexes, Institut für Organische Chemie und Chemische Biologie, J W Goethe Universität Frankfurt am Main, Max von Laue Strasse 7, D 60438 Frankfurt am Main, Germany
Mol Cell 30:26-38. 2008..The results suggest that L11 acts as a molecular switch to control L7 binding and plays a pivotal role in positioning one L7-CTD monomer on the G' subdomain of EF-G to regulate EF-G turnover during protein synthesis... - Structure of eEF3 and the mechanism of transfer RNA release from the E-siteChristian B F Andersen
Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK-8000 Aarhus, Denmark
Nature 443:663-8. 2006..eEF3 uses an entirely new factor binding site near the ribosomal E-site, with the chromodomain likely to stabilize the ribosomal L1 stalk in an open conformation, thus allowing tRNA release... - A method of focused classification, based on the bootstrap 3D variance analysis, and its application to EF-G-dependent translocationPawel A Penczek
Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, 6431 Fannin, MSB 6 218, Houston, TX 77030, USA
J Struct Biol 154:184-94. 2006..The associated calculation of the correlations within the density map confirmed the conformational variability of the complex, which could be interpreted in terms of the ribosomal elongation cycle... - Codon-anticodon interaction at the P site is a prerequisite for tRNA interaction with the small ribosomal subunitMarkus A Schäfer
Max Planck Institut fur Molekulare Genetik, AG Ribosomen, Ihnestrasse 73, D 14195 Berlin, Germany
J Biol Chem 277:19095-105. 2002..5-A crystal structure of a programmed 70 S ribosome, thus refining which ribosomal components are critical for tRNA fixation at the P site... - Estimation of variance in single-particle reconstruction using the bootstrap techniquePawel A Penczek
Department of Biochemistry and Molecular Biology, The University of Texas-Houston Medical School, 6431 Fannin, MSB 6.218, Houston, TX 77030, USA
J Struct Biol 154:168-83. 2006..Computations with test data confirm the viability, computational efficiency, and accuracy of the method under conditions encountered in practical circumstances... 
A European focus on proteomicsChristian M T Spahn
Institute of Medical Physics and Biophysics, Charit, Berlin, Germany
Genome Biol 5:322. 2004