Christian M T Spahn

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. pmc Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications
    Rajendra K Agrawal
    Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201 0509, USA
    Proc Natl Acad Sci U S A 101:8900-5. 2004
  2. pmc Exploring conformational modes of macromolecular assemblies by multiparticle cryo-EM
    Christian M T Spahn
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Curr Opin Struct Biol 19:623-31. 2009
  3. pmc Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation
    Christian M T Spahn
    Wadsworth Center, Health Research Inc, Howard Hughes Medical Institute, Albany, NY 12201 0509, USA
    EMBO J 23:1008-19. 2004
  4. pmc Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites
    Andreas H Ratje
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Nature 468:713-6. 2010
  5. doi request reprint The complex of tmRNA-SmpB and EF-G on translocating ribosomes
    David J F Ramrath
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Nature 485:526-9. 2012
  6. ncbi request reprint Structure of the ribosome-bound cricket paralysis virus IRES RNA
    Martin Schuler
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Nat Struct Mol Biol 13:1092-6. 2006
  7. ncbi request reprint A new tRNA intermediate revealed on the ribosome during EF4-mediated back-translocation
    Sean R Connell
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Nat Struct Mol Biol 15:910-5. 2008
  8. ncbi request reprint Structural basis for interaction of the ribosome with the switch regions of GTP-bound elongation factors
    Sean R Connell
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Mol Cell 25:751-64. 2007
  9. ncbi request reprint Localization and dynamic behavior of ribosomal protein L30e
    Mario Halic
    Institute of Biochemistry, Charite, University Medical School Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
    Nat Struct Mol Biol 12:467-8. 2005
  10. pmc GTPase activation of elongation factor EF-Tu by the ribosome during decoding
    Jan Christian Schuette
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Berlin, Germany
    EMBO J 28:755-65. 2009

Collaborators

Detail Information

Publications27

  1. pmc Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications
    Rajendra K Agrawal
    Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201 0509, USA
    Proc Natl Acad Sci U S A 101:8900-5. 2004
    ....
  2. pmc Exploring conformational modes of macromolecular assemblies by multiparticle cryo-EM
    Christian M T Spahn
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Curr Opin Struct Biol 19:623-31. 2009
    ..Their applications point to the future of cryo-EM as an experimental method uniquely suited to visualize the conformational modes of large macromolecular complexes and machines...
  3. pmc Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation
    Christian M T Spahn
    Wadsworth Center, Health Research Inc, Howard Hughes Medical Institute, Albany, NY 12201 0509, USA
    EMBO J 23:1008-19. 2004
    ..sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction...
  4. pmc Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites
    Andreas H Ratje
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Nature 468:713-6. 2010
    ..These findings provide direct structural and mechanistic insight into the 'missing link' in terms of tRNA intermediates involved in the universally conserved translocation process...
  5. doi request reprint The complex of tmRNA-SmpB and EF-G on translocating ribosomes
    David J F Ramrath
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Nature 485:526-9. 2012
    ....
  6. ncbi request reprint Structure of the ribosome-bound cricket paralysis virus IRES RNA
    Martin Schuler
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Nat Struct Mol Biol 13:1092-6. 2006
    ..Our results exemplify the molecular strategy for recruitment of an IRES and reveal the dynamic features necessary for internal initiation...
  7. ncbi request reprint A new tRNA intermediate revealed on the ribosome during EF4-mediated back-translocation
    Sean R Connell
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Nat Struct Mol Biol 15:910-5. 2008
    ..Our results, therefore, provide insight into the underlying structural principles governing back-translocation...
  8. ncbi request reprint Structural basis for interaction of the ribosome with the switch regions of GTP-bound elongation factors
    Sean R Connell
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Mol Cell 25:751-64. 2007
    ..Therefore, a network of interaction with the ribosome establishes the active GTP conformation of EF-G and thus facilitates GTP hydrolysis and tRNA translocation...
  9. ncbi request reprint Localization and dynamic behavior of ribosomal protein L30e
    Mario Halic
    Institute of Biochemistry, Charite, University Medical School Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
    Nat Struct Mol Biol 12:467-8. 2005
    ..5 A. L30e is part of the interface between large and small subunits, where it dynamically participates in the formation of the two intersubunit bridges eB9 and B4...
  10. pmc GTPase activation of elongation factor EF-Tu by the ribosome during decoding
    Jan Christian Schuette
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Berlin, Germany
    EMBO J 28:755-65. 2009
    ..Thus, the structure provides insights into the molecular mechanism of signalling codon recognition from the decoding centre of the 30S subunit to the GTPase centre of EF-Tu...
  11. ncbi request reprint Structure of the signal recognition particle interacting with the elongation-arrested ribosome
    Mario Halic
    Institute of Biochemistry, Charite, University Medical School, Humboldt University of Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
    Nature 427:808-14. 2004
    ....
  12. ncbi request reprint Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factor
    Christian M T Spahn
    Howard Hughes Medical Institute, Health Research Inc at, Albany, NY 10012, USA
    Cell 118:465-75. 2004
    ..Conformational changes in the ribosome as well as within the IRES itself show that CrPV IRES actively manipulates the ribosome. CrPV-like IRES elements seem to act as RNA-based translation factors...
  13. pmc Structure and dynamics of the mammalian ribosomal pretranslocation complex
    Tatyana Budkevich
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Mol Cell 44:214-24. 2011
    ....
  14. doi request reprint Multiparticle cryo-EM of ribosomes
    Justus Loerke
    Institut fur Medizinische Physik und Biophysik, Charite, Universitatsmedizin Berlin, Berlin, Germany
    Methods Enzymol 483:161-77. 2010
    ..This increases information gained from single states, while exploiting the coexistence of multiple states to gather comprehensive mechanistic insight into biological processes like ribosomal translocation...
  15. pmc Structural basis for the binding of IRES RNAs to the head of the ribosomal 40S subunit
    Margarita Muhs
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Nucleic Acids Res 39:5264-75. 2011
    ....
  16. ncbi request reprint Identification of the versatile scaffold protein RACK1 on the eukaryotic ribosome by cryo-EM
    Jayati Sengupta
    Health Research, Inc, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, New York 12201 0509, USA
    Nat Struct Mol Biol 11:957-62. 2004
    ..One face of RACK1 exposes the WD-repeats as a platform for interactions with kinases and receptors. Using this platform, RACK1 can recruit other proteins to the ribosome...
  17. doi request reprint Visualization of two transfer RNAs trapped in transit during elongation factor G-mediated translocation
    David J F Ramrath
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, 10117 Berlin, Germany
    Proc Natl Acad Sci U S A 110:20964-9. 2013
    ....
  18. doi request reprint EF-G and EF4: translocation and back-translocation on the bacterial ribosome
    Hiroshi Yamamoto
    1 Institut für Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany 2
    Nat Rev Microbiol 12:89-100. 2014
    ..In this Review, we describe recent insights into the mechanistic basis of both translocation and back-translocation. ..
  19. pmc A dose-rate effect in single-particle electron microscopy
    James Z Chen
    Howard Hughes Medical Institute, Rosenstiel Center MS029, Brandeis University, 415 South Street, Waltham, MA 02545 9110, USA
    J Struct Biol 161:92-100. 2008
    ..The improvement in image quality, especially for multiple-exposure data collection, will help single-particle cryo-EM to reach higher resolution...
  20. ncbi request reprint An assay for local quality in cryo-electron micrographs of single particles
    Haixiao Gao
    Howard Hughes Medical Institute, Wadsworth Center, Albany, New York 12201 0509, USA
    Ultramicroscopy 93:169-78. 2002
    ..The method of assaying, resulting in "local quality maps", may be useful as a general tool for screening micrographs used as input for reconstructions...
  21. ncbi request reprint Building functional modules from molecular interactions
    Klaus Peter Hofmann
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Campus Mitte, Chariteplatz 1, 10098 Berlin, Germany
    Trends Biochem Sci 31:497-508. 2006
    ..Further progress in this field, where systems biology and biochemistry meet, will depend on iterative validation of the experimental and theoretical approaches...
  22. doi request reprint Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin
    Joerg M Harms
    Cluster of Excellence for Macromolecular Complexes, Institut für Organische Chemie und Chemische Biologie, J W Goethe Universität Frankfurt am Main, Max von Laue Strasse 7, D 60438 Frankfurt am Main, Germany
    Mol Cell 30:26-38. 2008
    ..The results suggest that L11 acts as a molecular switch to control L7 binding and plays a pivotal role in positioning one L7-CTD monomer on the G' subdomain of EF-G to regulate EF-G turnover during protein synthesis...
  23. ncbi request reprint A method of focused classification, based on the bootstrap 3D variance analysis, and its application to EF-G-dependent translocation
    Pawel A Penczek
    Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, 6431 Fannin, MSB 6 218, Houston, TX 77030, USA
    J Struct Biol 154:184-94. 2006
    ..The associated calculation of the correlations within the density map confirmed the conformational variability of the complex, which could be interpreted in terms of the ribosomal elongation cycle...
  24. ncbi request reprint Structure of eEF3 and the mechanism of transfer RNA release from the E-site
    Christian B F Andersen
    Centre for Structural Biology, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK 8000 Aarhus, Denmark
    Nature 443:663-8. 2006
    ..eEF3 uses an entirely new factor binding site near the ribosomal E-site, with the chromodomain likely to stabilize the ribosomal L1 stalk in an open conformation, thus allowing tRNA release...
  25. ncbi request reprint Codon-anticodon interaction at the P site is a prerequisite for tRNA interaction with the small ribosomal subunit
    Markus A Schäfer
    Max Planck Institut fur Molekulare Genetik, AG Ribosomen, Ihnestrasse 73, D 14195 Berlin, Germany
    J Biol Chem 277:19095-105. 2002
    ..5-A crystal structure of a programmed 70 S ribosome, thus refining which ribosomal components are critical for tRNA fixation at the P site...
  26. ncbi request reprint Estimation of variance in single-particle reconstruction using the bootstrap technique
    Pawel A Penczek
    Department of Biochemistry and Molecular Biology, The University of Texas Houston Medical School, 6431 Fannin, MSB 6 218, Houston, TX 77030, USA
    J Struct Biol 154:168-83. 2006
    ..Computations with test data confirm the viability, computational efficiency, and accuracy of the method under conditions encountered in practical circumstances...
  27. pmc A European focus on proteomics
    Christian M T Spahn
    Institute of Medical Physics and Biophysics, Charite, Berlin, Germany
    Genome Biol 5:322. 2004