Affiliation: Humboldt University
Hövelmann F, Gaspar I, Chamiolo J, Kasper M, Steffen J, Ephrussi A, et al
. LNA-enhanced DNA FIT-probes for multicolour RNA imaging. Chem Sci. 2016;7:128-135 pubmed publisher
Fang G, Chamiolo J, Kankowski S, Hövelmann F, Friedrich D, Löwer A, et al
. A bright FIT-PNA hybridization probe for the hybridization state specific analysis of a C ? U RNA edit via FRET in a binary system. Chem Sci. 2018;9:4794-4800 pubmed publisher
Loibl S, Harpaz Z, Zitterbart R, Seitz O. Total chemical synthesis of proteins without HPLC purification. Chem Sci. 2016;7:6753-6759 pubmed publisher
..Only three days were required for the parallel synthesis of 9 MUC1 proteins which were obtained in 8-33% overall yield with 90-98% purity despite the omission of HPLC purification. ..
Lotze J, Reinhardt U, Seitz O, Beck Sickinger A. Peptide-tags for site-specific protein labelling in vitro and in vivo. Mol Biosyst. 2016;12:1731-45 pubmed publisher
Kern A, Seitz O. Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA. Chem Sci. 2015;6:724-728 pubmed publisher
..The combined use of native chemical PNA ligation and auxiliary DNA probes enabled reactions on templates obtained from human genomic DNA. ..
Schmalisch J, Seitz O. Acceleration of thiol additive-free native chemical ligation by intramolecular S â†’ S acyl transfer. Chem Commun (Camb). 2015;51:7554-7 pubmed publisher
..This facilitates sequential NCL reactions and ligation-desulfurization reactions in one-pot formats. The synthetic utility is demonstrated by the synthesis of a SH3 domain. ..
Diezmann F, von Kleist L, Haucke V, Seitz O. Probing heterobivalent binding to the endocytic AP-2 adaptor complex by DNA-based spatial screening. Org Biomol Chem. 2015;13:8008-15 pubmed publisher
..By contrast, the experiments with a more rigid, duplex-spaced assembly revealed marked distance dependencies. Consequences for the function of adaptor proteins are discussed. ..
Loibl S, Harpaz Z, Seitz O. A Type of Auxiliary for Native Chemical Peptide Ligation beyond Cysteine and Glycine Junctions. Angew Chem Int Ed Engl. 2015;54:15055-9 pubmed publisher
..The synthetic utility of the method is demonstrated by the synthesis of two antimicrobial proteins, DCD-1L and opistoporin-2. ..
Zitterbart R, Seitz O. Parallel Chemical Protein Synthesis on a Surface Enables the Rapid Analysis of the Phosphoregulation of SH3 Domains. Angew Chem Int Ed Engl. 2016;55:7252-6 pubmed publisher
..The analysis suggests that tyrosine phosphorylation of SH3 domains found in Abl kinases act as a switch that can induce both the loss and, unexpectedly, gain of affinity for proline-rich ligands. ..