Jörg Dieter Schulzke

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. pmc The plant-derived glucocorticoid receptor agonist Endiandrin A acts as co-stimulator of colonic epithelial sodium channels (ENaC) via SGK-1 and MAPKs
    Dana Kuntzsch
    Department of Gastroenterology, Division of Nutritional Medicine, Charite, Campus Benjamin Franklin, Berlin, Germany
    PLoS ONE 7:e49426. 2012
  2. pmc Oral and fecal Campylobacter concisus strains perturb barrier function by apoptosis induction in HT-29/B6 intestinal epithelial cells
    Hans Linde Nielsen
    Department of Infectious Diseases, Aalborg Hospital, Aarhus University Hospital, Aalborg, Denmark
    PLoS ONE 6:e23858. 2011
  3. doi request reprint Perspectives on tight junction research
    Jörg Dieter Schulzke
    Department of Gastroenterology, Infectious Diseases, and Rheumatology, Division of Nutritional Medicine, Charite, Universitatsmedizin Berlin, Germany
    Ann N Y Acad Sci 1257:1-19. 2012
  4. doi request reprint Prostaglandin I(2) sensory input into the enteric nervous system during distension-induced colonic chloride secretion in rat colon
    J D Schulzke
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Charite, Berlin, Germany
    Acta Physiol (Oxf) 199:305-16. 2010
  5. pmc Anti-diarrheal mechanism of the traditional remedy Uzara via reduction of active chloride secretion
    Jörg D Schulzke
    Department of Gastroenterology, Section of General Medicine and Nutrition, Charite, Campus Benjamin Franklin, Freie Universität Berlin and Humboldt Universität zu Berlin, Berlin, Germany
    PLoS ONE 6:e18107. 2011
  6. doi request reprint Cell polarity-determining proteins Par-3 and PP-1 are involved in epithelial tight junction defects in coeliac disease
    Michael Schumann
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Campus Benjamin Franklin, Charite Berlin, Berlin, Germany
    Gut 61:220-8. 2012
  7. pmc Downregulation of epithelial apoptosis and barrier repair in active Crohn's disease by tumour necrosis factor alpha antibody treatment
    S Zeissig
    Medizinische Klinik I, Charité Universitary Medicine Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, Berlin 12200, Germany
    Gut 53:1295-302. 2004
  8. doi request reprint Epithelial tight junctions in intestinal inflammation
    Joerg D Schulzke
    Department of General Medicine and Pathophysiology of Enteral Nutrition, Charite, Campus Benjamin Franklin, Berlin, Germany
    Ann N Y Acad Sci 1165:294-300. 2009
  9. ncbi request reprint Epithelial transport and barrier function in occludin-deficient mice
    J D Schulzke
    Department of Gastroenterology, Campus Benjamin Franklin, Charité Universitary Medicine Berlin, Germany
    Biochim Biophys Acta 1669:34-42. 2005
  10. ncbi request reprint Disrupted barrier function through epithelial cell apoptosis
    Joerg Dieter Schulzke
    Medizinische Klinik I, Department of Gastroenterology, Infectious Diseases and Rheumatology, Charite, Campus Benjamin Franklin, 12200 Berlin, Germany
    Ann N Y Acad Sci 1072:288-99. 2006

Collaborators

Detail Information

Publications78

  1. pmc The plant-derived glucocorticoid receptor agonist Endiandrin A acts as co-stimulator of colonic epithelial sodium channels (ENaC) via SGK-1 and MAPKs
    Dana Kuntzsch
    Department of Gastroenterology, Division of Nutritional Medicine, Charite, Campus Benjamin Franklin, Berlin, Germany
    PLoS ONE 7:e49426. 2012
    ....
  2. pmc Oral and fecal Campylobacter concisus strains perturb barrier function by apoptosis induction in HT-29/B6 intestinal epithelial cells
    Hans Linde Nielsen
    Department of Infectious Diseases, Aalborg Hospital, Aarhus University Hospital, Aalborg, Denmark
    PLoS ONE 6:e23858. 2011
    ..concisus strains could mainly be assigned to apoptotic leaks together with moderate TJ changes, demonstrating a leak-flux mechanism that parallels the clinical manifestation of diarrhea...
  3. doi request reprint Perspectives on tight junction research
    Jörg Dieter Schulzke
    Department of Gastroenterology, Infectious Diseases, and Rheumatology, Division of Nutritional Medicine, Charite, Universitatsmedizin Berlin, Germany
    Ann N Y Acad Sci 1257:1-19. 2012
    ..This overview concludes with a list of open questions and future topics in tight junction research...
  4. doi request reprint Prostaglandin I(2) sensory input into the enteric nervous system during distension-induced colonic chloride secretion in rat colon
    J D Schulzke
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Charite, Berlin, Germany
    Acta Physiol (Oxf) 199:305-16. 2010
    ..This study aimed to investigate sensorial inputs and the role of afferent and interneurones in mechanically activated submucosal secretory reflex circuits...
  5. pmc Anti-diarrheal mechanism of the traditional remedy Uzara via reduction of active chloride secretion
    Jörg D Schulzke
    Department of Gastroenterology, Section of General Medicine and Nutrition, Charite, Campus Benjamin Franklin, Freie Universität Berlin and Humboldt Universität zu Berlin, Berlin, Germany
    PLoS ONE 6:e18107. 2011
    ..The root extract of the African Uzara plant is used in traditional medicine as anti-diarrheal drug. It is known to act via inhibition of intestinal motility, but malabsorptive or antisecretory mechanisms are unknown yet...
  6. doi request reprint Cell polarity-determining proteins Par-3 and PP-1 are involved in epithelial tight junction defects in coeliac disease
    Michael Schumann
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Campus Benjamin Franklin, Charite Berlin, Berlin, Germany
    Gut 61:220-8. 2012
    ..Epithelial barrier defects are well known in coeliac disease, but the mechanisms are only poorly defined. It is unclear, whether barrier disturbance reflects upregulated epithelial transcytosis or paracellular leakage...
  7. pmc Downregulation of epithelial apoptosis and barrier repair in active Crohn's disease by tumour necrosis factor alpha antibody treatment
    S Zeissig
    Medizinische Klinik I, Charité Universitary Medicine Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, Berlin 12200, Germany
    Gut 53:1295-302. 2004
    ..Recently, tumour necrosis factor alpha (TNF-alpha) antibodies were recognised as effective in steroid refractory CD. The aim of this study was to characterise the effects of this therapy on the epithelial barrier...
  8. doi request reprint Epithelial tight junctions in intestinal inflammation
    Joerg D Schulzke
    Department of General Medicine and Pathophysiology of Enteral Nutrition, Charite, Campus Benjamin Franklin, Berlin, Germany
    Ann N Y Acad Sci 1165:294-300. 2009
    ..Immune regulation of epithelial functions by cytokines may cause barrier dysfunction not only by tight junction impairments but also by apoptotic leaks, transcytotic mechanisms, and mucosal gross lesions...
  9. ncbi request reprint Epithelial transport and barrier function in occludin-deficient mice
    J D Schulzke
    Department of Gastroenterology, Campus Benjamin Franklin, Charité Universitary Medicine Berlin, Germany
    Biochim Biophys Acta 1669:34-42. 2005
    ..This study aimed at functional characterization of the tight junction protein occludin using the occludin-deficient mouse model...
  10. ncbi request reprint Disrupted barrier function through epithelial cell apoptosis
    Joerg Dieter Schulzke
    Medizinische Klinik I, Department of Gastroenterology, Infectious Diseases and Rheumatology, Charite, Campus Benjamin Franklin, 12200 Berlin, Germany
    Ann N Y Acad Sci 1072:288-99. 2006
    ..Therapeutically,TNF-alpha-antibodies (infliximab) can restore barrier function in Crohn's disease by downregulating epithelial apoptoses, while epithelial TJs are unaffected...
  11. doi request reprint Disorders of intestinal secretion and absorption
    Jörg Dieter Schulzke
    Department of Gastroenterology, Infectiology, and Rheumatology, Hindenburgdamm 30, 12203 Berlin, Germany
    Best Pract Res Clin Gastroenterol 23:395-406. 2009
    ..g. for iron or vitamins) and protein-losing enteropathy are presented with obligatory and optional tests as used in the clinical setting...
  12. ncbi request reprint Supernatants of HIV-infected immune cells affect the barrier function of human HT-29/B6 intestinal epithelial cells
    Heinz Schmitz
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, 12200 Berlin, Germany
    AIDS 16:983-91. 2002
    ..Characterization of the diarrhoea-inducing effect of altered cytokine production in HIV infection...
  13. ncbi request reprint Contribution of claudin-5 to barrier properties in tight junctions of epithelial cells
    Salah Amasheh
    Department of Clinical Physiology, Charité Universitary Medicine Berlin, Campus Benjamin Franklin, 12200 Berlin, Germany
    Cell Tissue Res 321:89-96. 2005
    ..Claudin-5 may thus be classified as a tight-junctional protein capable of contributing to the "sealing" of the tight junction...
  14. ncbi request reprint The specific fates of tight junction proteins in apoptotic epithelial cells
    Christian Bojarski
    Institute of Clinical Chemistry and Pathobiochemistry, Charite Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
    J Cell Sci 117:2097-107. 2004
    ..While the amount of claudin-2 protein was reduced similarly to occludin, ZO-1 and ZO-2, claudin-1 was either fully preserved or was even increased in apoptotic cells...
  15. doi request reprint Altered ENaC expression leads to impaired sodium absorption in the noninflamed intestine in Crohn's disease
    Sebastian Zeissig
    Department of Gastroenterology, Infectious Diseases, and Rheumatology, Charite, Campus Benjamin Franklin, Berlin, Germany
    Gastroenterology 134:1436-47. 2008
    ..Crohn's disease (CD) is a chronic inflammatory bowel disease. In this study, we have investigated sodium absorption via epithelial sodium channels (ENaC) in the macroscopically noninflamed colon in active CD...
  16. ncbi request reprint Cytokine-dependent transcriptional down-regulation of epithelial sodium channel in ulcerative colitis
    Salah Amasheh
    Department of Clinical Physiology, Campus Benjamin Franklin, Charite University Medicine Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
    Gastroenterology 126:1711-20. 2004
    ..This study aimed to characterize mechanisms involved in the dysregulation of ENaC expression in ulcerative colitis (UC)...
  17. doi request reprint The effect of chitosan on transcellular and paracellular mechanisms in the intestinal epithelial barrier
    Rita Rosenthal
    Institute of Clinical Physiology, Charite, Campus Benjamin Franklin, Freie Universität and Humboldt Universität, 12200 Berlin, Germany
    Biomaterials 33:2791-800. 2012
    ..These results suggest that the rapid and reversible absorption-enhancing chitosan effect is due to changes in intracellular pH caused by the activation of a chloride-bicarbonate exchanger resulting in the opening of the TJ...
  18. doi request reprint Aerolysin from Aeromonas hydrophila perturbs tight junction integrity and cell lesion repair in intestinal epithelial HT-29/B6 cells
    Roland Bücker
    Department of Gastroenterology, Infectious Diseases, and Rheumotology, Charite Berlin, Germany
    J Infect Dis 204:1283-92. 2011
    ..Pathogenesis of Aeromonas hydrophila and its hemolysin has been characterized, but the mechanism of the epithelial barrier dysfunction is currently poorly understood...
  19. ncbi request reprint IL-1beta and TNFalpha regulate sodium absorption in rat distal colon
    Christian Barmeyer
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Campus Benjamin Franklin, Charite University Medicine Berlin, 12200 Berlin, Germany
    Biochem Biophys Res Commun 317:500-7. 2004
    ..We conclude that the pro-inflammatory cytokines IL-1beta and TNFalpha inhibit electrogenic sodium absorption in rat distal colon by mRNA expression regulation of the beta- and gamma-ENaC subunits...
  20. ncbi request reprint Butyrate induces intestinal sodium absorption via Sp3-mediated transcriptional up-regulation of epithelial sodium channels
    Sebastian Zeissig
    Department of Gastroenterology, Infectious Diseases, and Rheumatology, Charite, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
    Gastroenterology 132:236-48. 2007
    ..The epithelial sodium channel (ENaC) is the rate-limiting factor for colonic electrogenic sodium absorption. This study aimed to investigate ENaC regulation by butyrate, a short-chain fatty acid (SCFA) produced by intestinal bacteria...
  21. ncbi request reprint Mechanisms of diarrhea in collagenous colitis
    Natalie Bürgel
    Department of Gastroenterology, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Berlin, Germany
    Gastroenterology 123:433-43. 2002
    ..Collagenous colitis is an inflammatory disease of unknown etiology with diarrhea as the leading symptom. The aim of this study was to examine the pathogenic mechanisms of this disease...
  22. doi request reprint Claudin-3 acts as a sealing component of the tight junction for ions of either charge and uncharged solutes
    Susanne Milatz
    Institute of Clinical Physiology, Charite, Campus Benjamin Franklin, Berlin, Germany
    Biochim Biophys Acta 1798:2048-57. 2010
    ..Thus, in a kidney model epithelium, claudin-3 acts as a general barrier-forming protein...
  23. doi request reprint Claudin-2, a component of the tight junction, forms a paracellular water channel
    Rita Rosenthal
    Institute of Clinical Physiology, Charite, Campus Benjamin Franklin, Freie Universität and Humboldt Universität, 12200 Berlin, Germany
    J Cell Sci 123:1913-21. 2010
    ..By contrast, claudin-10b transfection did not alter water flux. We conclude that claudin-2, but not claudin-10b, forms a paracellular water channel and thus mediates paracellular water transport in leaky epithelia...
  24. ncbi request reprint Escherichia coli alpha-haemolysin induces focal leaks in colonic epithelium: a novel mechanism of bacterial translocation
    Hanno Troeger
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Campus Benjamin Franklin, Charite, Berlin, Germany
    Cell Microbiol 9:2530-40. 2007
    ..E. coli alpha-haemolysin is able to induce focal leaks in colonic cell cultures as well as in native colon. This process represents a novel route of bacterial translocation facilitated by pro-inflammatory cytokines...
  25. doi request reprint Barrier effects of nutritional factors
    Maren Amasheh
    Department of Gastroenterology, Charite, Campus Benjamin Franklin, Berlin, Germany
    Ann N Y Acad Sci 1165:267-73. 2009
    ..This is due to a 2.3 +/- 0.1-fold expression rate of the tight junction protein claudin-4...
  26. doi request reprint Regulation of mucosal structure and barrier function in rat colon exposed to tumor necrosis factor alpha and interferon gamma in vitro: a novel model for studying the pathomechanisms of inflammatory bowel disease cytokines
    Maren Amasheh
    Department of Medicine I Gastroenterology, Infectious Diseases and Rheumatology, Charite, Campus Benjamin Franklin, Berlin, Germany
    Scand J Gastroenterol 44:1226-35. 2009
    ....
  27. doi request reprint Na+ absorption defends from paracellular back-leakage by claudin-8 upregulation
    Salah Amasheh
    Institute of Clinical Physiology, Charite, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
    Biochem Biophys Res Commun 378:45-50. 2009
    ..These results represent an important physiological implication: Na(+) absorption is paralleled by claudin-8-mediated sealing of the paracellular barrier to prevent Na(+) back-leakage, supporting steep Na(+) gradients in distal colon...
  28. doi request reprint TNFalpha-induced and berberine-antagonized tight junction barrier impairment via tyrosine kinase, Akt and NFkappaB signaling
    Maren Amasheh
    Department of Gastroenterology, Charite, Campus Benjamin Franklin, Berlin 12200, Germany
    J Cell Sci 123:4145-55. 2010
    ..In conclusion, the anti-diarrheal effect of berberine is explained by a novel mechanism, suggesting a therapeutic approach against barrier breakdown in intestinal inflammation...
  29. ncbi request reprint Arcobacter butzleri induces barrier dysfunction in intestinal HT-29/B6 cells
    Roland Bücker
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Charite Berlin, Berlin, Germany
    J Infect Dis 200:756-64. 2009
    ..Arcobacter butzleri causes watery diarrhea and bacteremia. Although, recently, more cases of diarrhea have been caused by Arcobacter species, very little is known about its pathogenesis, the identification of which is the aim of this study...
  30. ncbi request reprint TRPV4-mediated regulation of epithelial permeability
    Bettina Reiter
    Institut fur Pharmakologie, Charite Campus Benjamin Franklin, Berlin, Germany
    FASEB J 20:1802-12. 2006
    ..Reiter, B., Kraft, R., Günzel, D., Zeissig, S., Schulzke, J-D., Fromm, M., Harteneck, C. TRPV4-mediated regulation of epithelial permeability...
  31. doi request reprint Ion transport and barrier function are disturbed in microscopic colitis
    Christian Barmeyer
    Department of Gastroenterology, Infectiology and Rheumatology, Charite, Campus Benjamin Franklin, Freie Universität and Humboldt Universität, Berlin, Germany
    Ann N Y Acad Sci 1258:143-8. 2012
    ..From a therapeutic perspective it can be postulated that the functional importance of loss of ions may be higher than that caused by barrier impairment...
  32. doi request reprint High-resolution analysis of barrier function
    Michael Fromm
    Institute of Clinical Physiology, Charite, Berlin, Germany
    Ann N Y Acad Sci 1165:74-81. 2009
    ..Third, three-dimensional confocal fluorescence imaging for depicting transepithelial transport processes is introduced. Using this method the transepithelial translocation of bacteria which generate focal leaks was discovered...
  33. doi request reprint Acute HIV infection induces mucosal infiltration with CD4+ and CD8+ T cells, epithelial apoptosis, and a mucosal barrier defect
    Hans Jörg Epple
    Medical Clinic I, Gastroenterology, Rheumatology, Infectiology, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, Berlin, Germany
    Gastroenterology 139:1289-300. 2010
    ..We analyzed T-cell subsets, epithelial apoptosis, and barrier function of the duodenal mucosa in patients with acute HIV infection...
  34. doi request reprint Defective tight junctions in refractory celiac disease
    Michael Schumann
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Berlin, Germany
    Ann N Y Acad Sci 1258:43-51. 2012
    ..Furthermore, the tightening claudin-5 is downregulated and the pore-forming claudin-2 is upregulated...
  35. doi request reprint Effects of quercetin studied in colonic HT-29/B6 cells and rat intestine in vitro
    Maren Amasheh
    Department of Gastroenterology, Infectious Diseases, and Rheumatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Ann N Y Acad Sci 1258:100-7. 2012
    ..These findings demonstrate that quercetin enhances barrier function in rat small and large intestine and possesses protective effects on cytokine-induced barrier damage...
  36. ncbi request reprint Predictors of clinical response to gluten-free diet in patients diagnosed with diarrhea-predominant irritable bowel syndrome
    Ulrich Wahnschaffe
    Medical Clinic A, Department of Gastroenterology, Endocrinology and Nutritrion, University Hospital Ernst Moritz Arndt Universität Greifswald, Greifswald, Germany
    Clin Gastroenterol Hepatol 5:844-50; quiz 769. 2007
    ....
  37. ncbi request reprint Altered permeability in inflammatory bowel disease: pathophysiology and clinical implications
    Joachim Mankertz
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Charite Campus Benjamin Franklin, Berlin, Germany
    Curr Opin Gastroenterol 23:379-83. 2007
    ..To present the mechanisms behind barrier disturbance in inflammatory bowel disease and their functional consequences...
  38. doi request reprint Nano- and microscaled particles for drug targeting to inflamed intestinal mucosa: a first in vivo study in human patients
    Carsten Schmidt
    Clinic of Internal Medicine II, Department of Gastroenterology, Hepatology and Infectious Diseases, University Clinic Jena, Jena, Germany
    J Control Release 165:139-45. 2013
    ..Nanoparticles may not be required for local drug delivery to intestinal lesions in humans, thereby minimising the risk of unintended translocation into the blood system...
  39. pmc Determinants of colonic barrier function in inflammatory bowel disease and potential therapeutics
    Nina A Hering
    Department of Gastroenterology, Division of Nutritional Medicine, Charite, Campus Benjamin Franklin, Berlin, Germany
    J Physiol 590:1035-44. 2012
    ..Beside cytokine antagonists, different plant compounds and probiotics have been shown to stabilize the barrier function by affecting TJ protein expression and distribution...
  40. doi request reprint Impairment of the intestinal barrier is evident in untreated but absent in suppressively treated HIV-infected patients
    H J Epple
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Gut 58:220-7. 2009
    ..The purpose of this study was to investigate the effect of highly active antiretroviral therapy (HAART) on the HIV-induced intestinal barrier defect and to identify underlying mechanisms...
  41. ncbi request reprint Mechanisms of diarrhea in the interleukin-2-deficient mouse model of colonic inflammation
    C Barmeyer
    Department of Gastroenterology, Charite University Medicine Berlin, 12200 Berlin, Germany
    Am J Physiol Gastrointest Liver Physiol 286:G244-52. 2004
    ..Therefore, this model is ideal to study regulation of transporter expression independent of barrier defects...
  42. doi request reprint Epithelial barriers in intestinal inflammation
    Lena J John
    Department of General Medicine, Charite, Campus Benjamin Franklin, Berlin, Germany
    Antioxid Redox Signal 15:1255-70. 2011
    ..In addition, the main cytokines responsible for these effects and their role during oxidative stress and intestinal inflammation will be discussed, as well as therapeutic approaches and their mode of action...
  43. doi request reprint Norovirus non-structural protein p20 leads to impaired restitution of epithelial defects by inhibition of actin cytoskeleton remodelling
    Bernd Hillenbrand
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Scand J Gastroenterol 45:1307-19. 2010
    ..Because of the lack of cell lines susceptible to human norovirus infection, pathomechanisms and replication cycle are largely unknown. Here, we address the issue of how norovirus infection could lead to epithelial barrier dysfunction...
  44. doi request reprint Surveillance colonoscopy in patients with inflammatory bowel disease: comparison of random biopsy vs. targeted biopsy protocols
    Ute Gunther
    Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Medizinische Klinik I Gastroenterologie, Infektiologie, Rheumatologie, Hindenburgdamm 30, 12200 Berlin, Germany
    Int J Colorectal Dis 26:667-72. 2011
    ..We aimed to compare three different endoscopic surveillance strategies in the detection of IEN...
  45. ncbi request reprint Functional crosstalk between Wnt signaling and Cdx-related transcriptional activation in the regulation of the claudin-2 promoter activity
    Joachim Mankertz
    Department of Gastroenterology, Infectiology and Rheumatology, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200, Berlin, Germany
    Biochem Biophys Res Commun 314:1001-7. 2004
    ..Moreover, a functional crosstalk between Wnt signaling and transcriptional activation related to caudal-related homeobox (Cdx) proteins could be demonstrated in the regulation of claudin-2 promoter-mediated gene expression...
  46. pmc Single-cell epithelial defects close rapidly by an actinomyosin purse string mechanism with functional tight junctions
    P Florian
    Institut für Klinische Physiologie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, 12200 Berlin, Germany
    J Physiol 545:485-99. 2002
    ....
  47. ncbi request reprint Leaks in the epithelial barrier caused by spontaneous and TNF-alpha-induced single-cell apoptosis
    A H Gitter
    Institute of Clinical Physiology, Department of Gastroenterology, University Hospital Benjamin Franklin, Freie Universitat Berlin, Berlin, Germany
    FASEB J 14:1749-53. 2000
    ..Hence, spontaneous and induced apoptosis hollow out the intestinal barrier and may facilitate loss of solutes and uptake of noxious agents...
  48. ncbi request reprint Epithelial barrier defects in HT-29/B6 colonic cell monolayers induced by tumor necrosis factor-alpha
    A H Gitter
    Institut für Klinische Physiologie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, 12200 Berlin, Germany
    Ann N Y Acad Sci 915:193-203. 2000
    ..These mechanisms explain clinical manifestations in the inflamed intestinal wall containing cytokine-secreting macrophages--for example, leak flux diarrhea and invasion of bacterial enterotoxins...
  49. pmc Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn's disease
    S Zeissig
    Department of Gastroenterology, Charite, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
    Gut 56:61-72. 2007
    ..Epithelial barrier function is impaired in Crohn's disease...
  50. doi request reprint Therapeutic options to modulate barrier defects in inflammatory bowel disease
    Nina A Hering
    Department of General Medicine, Charité Center 10, Campus Benjamin Franklin, Berlin, Germany
    Dig Dis 27:450-4. 2009
    ..Finally, food components can strengthen the epithelial barrier as for example the flavonoid quercetin which has been shown to upregulate claudin-4 within the epithelial TJ...
  51. ncbi request reprint The HIV protease inhibitors saquinavir, ritonavir, and nelfinavir induce apoptosis and decrease barrier function in human intestinal epithelial cells
    Hagen Bode
    Department of Gastroenterology Infectious Diseases, Campus Benjamin Franklin, Charite Universitatsmedizin Berlin, Berlin, Germany
    Antivir Ther 10:645-55. 2005
    ..We investigated whether tight junction dysregulation, apoptosis or necrosis are responsible for this epithelial damage...
  52. doi request reprint Through the endoscope balloon dilation of ileocolonic strictures: prognostic factors, complications, and effectiveness
    Jorg C Hoffmann
    Medizinische Klinik I mit Schwerpunkt Gastroenterologie, Infektiologie, Rheumatologie, Charite, Campus Benjamin Franklin, 12200 Berlin, Germany
    Int J Colorectal Dis 23:689-96. 2008
    ..About half of all Crohn's disease (CD) patients undergo surgery at some point, many because of strictures. An alternative possibility is to dilate strictures endoscopically. However, little is known about prognostic factors...
  53. ncbi request reprint The interleukin-2-deficient mouse model
    C Barmeyer
    Medical Clinic I Gastroenterology, Infectious Diseases and Rheumatology, University Clinic Benjamin Franklin, Free University of Berlin, Berlin, Germany
    Pathobiology 70:139-42. 2002
    ..This makes this model suitable for studying the general pathomechanisms of the inflammatory downregulation of intestinal transport proteins...
  54. ncbi request reprint TNFalpha up-regulates claudin-2 expression in epithelial HT-29/B6 cells via phosphatidylinositol-3-kinase signaling
    J Mankertz
    Department of Gastroenterology, Charite, Campus Benjamin Franklin, Hindenburgdamm 30, 12200, Berlin, Germany
    Cell Tissue Res 336:67-77. 2009
    ..Thus, the up-regulation of claudin-2 by TNFalpha is attributable to the regulation of the expression of the gene, as a result of which epithelial barrier function is disturbed, for example, during chronic intestinal inflammation...
  55. ncbi request reprint Expression from the human occludin promoter is affected by tumor necrosis factor alpha and interferon gamma
    J Mankertz
    Medizinische Klinik I Gastroenterologie und Infektiologie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Hindenburgdamm 30, Germany
    J Cell Sci 113:2085-90. 2000
    ..This could be an important mechanism in gastrointestinal diseases accompanied by barrier defects, for example inflammatory bowel diseases...
  56. ncbi request reprint Restitution of single-cell defects in the mouse colon epithelium differs from that of cultured cells
    D Gunzel
    Department of Clinical Physiology, Charite, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, and Jena University of Applied Sciences, Germany
    Am J Physiol Regul Integr Comp Physiol 290:R1496-507. 2006
    ..Proinflammatory cytokines and pathogenic bacteria delay restitution. This suggests a key role of very small lesions at the onset of pathogenic processes in the intestine...
  57. pmc Determinants of successful weight loss after using a commercial web-based weight reduction program for six months: cohort study
    Elisa Postrach
    The Division of Nutritional Medicine, Department of Gastroenterology and Hepatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    J Med Internet Res 15:e219. 2013
    ..KiloCoach, a commercial program currently available in German-speaking countries, incorporates these features. A previous investigation showed that the program effectively supports users in losing weight...
  58. ncbi request reprint Therapy with budesonide in patients with refractory sprue
    Severin Daum
    Department of Medicine I, Gastroenterology, Rheumatology and Infectious Diseases, Charité Universitary Medicine Berlin, Campus Benjamin Franklin, Berlin, Germany
    Digestion 73:60-8. 2006
    ..Locally acting budesonide could replace systemic corticosteroid therapy and reduce toxicity in patients with RS. Aims: To evaluate the efficacy and toxicity of budesonide in patients with RS...
  59. ncbi request reprint Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART
    Hans Jörg Epple
    Medical Clinic I, Gastroenterology, Rheumatology, Infectiology Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
    Blood 108:3072-8. 2006
    ..Their role may be 2-edged: attenuating HIV-induced immune hyperactivation while suppressing the immune response to HIV and mucosal pathogens...
  60. doi request reprint Serum bile acids and leptin interact with glucose metabolism in patients with liver cirrhosis
    Luzia Valentini
    Charite Universitatsmedizin Berlin, Department of Gastroenterology, Infectiology and Rheumatology incl section on Clinical Nutrition, Chariteplatz 1, Berlin 10117, Germany
    Clin Nutr 32:122-9. 2013
    ..We investigated possible involvements of bile acids (BA) and leptin in hepatogenous insulin resistance being present in up to 90% of cirrhotic patients...
  61. doi request reprint Microbial butyrate and its role for barrier function in the gastrointestinal tract
    Svenja Plöger
    Institute of Veterinary Physiology, Free University of Berlin, Berlin, Germany
    Ann N Y Acad Sci 1258:52-9. 2012
    ..Further evaluation of the molecular events that link butyrate to an improved tight junction structure will allow for the elucidation of the cofactors affecting the reliability of butyrate as a clinical treatment tool...
  62. doi request reprint Enhanced T cell transmigration across the murine liver sinusoidal endothelium is mediated by transcytosis and surface presentation of chemokines
    Arnhild Schrage
    Medizinische Klinik I, Campus Charité Benjamin Franklin, Berlin, Germany
    Hepatology 48:1262-72. 2008
    ..Blockage of transcytosis in LSEC significantly inhibited this effect, and this suggested that chemokines taken up from the basolateral side and presented on the luminal side of endothelial cells trigger T cell transmigration...
  63. doi request reprint Tight junctions: molecular structure meets function
    Jörg Dieter Schulzke
    Department of General Medicine, Campus Benjamin Franklin, Freie Universität and Humboldt Universität, Berlin, Germany
    Ann N Y Acad Sci 1165:1-6. 2009
    ....
  64. pmc Permeability of human HT-29/B6 colonic epithelium as a function of apoptosis
    C Bojarski
    Department of Gastroenterology, , , 12200 Berlin, Germany
    J Physiol 535:541-52. 2001
    ..Under control conditions the paracellular conductivity (G(para)) was smaller than the transcellular (G(trans)), but with 12 % apoptosis, G(para) exceeded G(trans). By definition, the epithelium became 'leaky'...
  65. doi request reprint Mechanisms of epithelial translocation of the alpha(2)-gliadin-33mer in coeliac sprue
    M Schumann
    Klinik fur Gastroenterologie, Charite, Campus Benjamin Franklin, 12200 Berlin, Germany
    Gut 57:747-54. 2008
    ..The alpha(2)-gliadin-33mer has been shown to be important in the pathogenesis of coeliac disease. We aimed to study mechanisms of its epithelial translocation and processing in respect to transcytotic and paracellular pathways...
  66. ncbi request reprint Epithelial apoptosis is a prominent feature of the epithelial barrier disturbance in intestinal inflammation: effect of pro-inflammatory interleukin-13 on epithelial cell function
    F Heller
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Charite, Campus Benjamin Franklin, Berlin, Germany
    Mucosal Immunol 1:S58-61. 2008
    ..In conclusion, IL-13 disturbs intestinal barrier function through mechanisms including apoptosis induction and alteration of tight junction protein composition...
  67. doi request reprint Determinants of bioelectrical phase angle in disease
    Nicole Stobäus
    Department of General Medicine, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Germany
    Br J Nutr 107:1217-20. 2012
    ..When investigating the SPhA, only malnutrition and inflammation were found to be significant predictors, as a result of which the SPhA is considered a more suitable indicator of nutritional and health status...
  68. doi request reprint Mundane, yet challenging: the assessment of malnutrition in inflammatory bowel disease
    Luzia Valentini
    Dept Gastroenterology, Hepatology and Endocrinology, Charite Universitatsmedizin Berlin, CCM, Chariteplatz 1, 10117 Berlin, Germany
    Eur J Intern Med 22:13-5. 2011
    ..Patients with active inflammatory bowel disease (IBD) are a high risk group for protein-energy malnutrition, with a prevalence ranging up to 75%...
  69. ncbi request reprint Gene expression of the tight junction protein occludin includes differential splicing and alternative promoter usage
    Joachim Mankertz
    Medizinische Klinik I Gastroenterologie, Infektiologie und Rheumatologie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Hindenburgdamm 30, 12000 Berlin, Germany
    Biochem Biophys Res Commun 298:657-66. 2002
    ..Gene expression mediated by this promoter is influenced by the pro-inflammatory cytokine tumor necrosis factor alpha...
  70. doi request reprint Structural and functional changes of the duodenum in human norovirus infection
    H Troeger
    Department of Gastroenterology, Infectious Diseases and Rheumatology, Campus Benjamin Franklin, Charite Universitatsmedizin, Berlin, Germany
    Gut 58:1070-7. 2009
    ..Norovirus infection is the most frequent cause of infectious diarrhoea in the western world. This study aimed to characterise functionally and histomorphologically the diseased duodenum in human biopsies...
  71. pmc Aeromonas hydrophila beta-hemolysin induces active chloride secretion in colon epithelial cells (HT-29/B6)
    H J Epple
    Medical Clinic I, Gastroenterology, Infectiology, and Rheumatology, Berlin, Germany
    Infect Immun 72:4848-58. 2004
    ..strain Sb. In conclusion, beta-hemolysin secreted by pathogenic aeromonads induces active Cl(-) secretion in the intestinal epithelium, possibly by channel insertion into the apical membrane and by activation of protein kinase C...
  72. ncbi request reprint Does miniprobe endoscopic ultrasound have a role in the diagnostic repertoire for colorectal cancer?
    Jörg Dieter Schulzke
    Gastroenterologie, Infektiologie und Rheumatologie, Medizinische Klinik I, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
    Int J Colorectal Dis 18:450. 2003
  73. doi request reprint The BASA-ROT table: an arithmetic-hypothetical concept for easy BMI-, age-, and sex-adjusted bedside estimation of energy expenditure
    Luzia Valentini
    Charite Universitatsmedizin Berlin, Berlin, Germany
    Nutrition 28:773-8. 2012
    ..We thus scrutinized the impact of BMI, age, and sex on ROTs, compared the results to the often used 25 kcal/kg ROT, and calculated a BMI-, age-, and sex-adjusted ROT table containing calories per kilogram in the basal state...
  74. doi request reprint Hand grip strength: outcome predictor and marker of nutritional status
    Kristina Norman
    Department of Gastroenterology, Hepatology and Endocrinology, Charite University Medicine Berlin, Berlin, Germany
    Clin Nutr 30:135-42. 2011
    ..This review outlines the prognostic relevance of grip strength in various clinical and epidemiologic settings and investigates its suitability as marker of nutritional status in cross-sectional as well as intervention studies...
  75. ncbi request reprint Epithelial barrier defects in ulcerative colitis: characterization and quantification by electrophysiological imaging
    A H Gitter
    Institut für Klinische Physiologie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, 12200 Berlin, Germany
    Gastroenterology 121:1320-8. 2001
    ..We quantified the contribution of these mechanisms to the increased colonic ion permeability...
  76. ncbi request reprint Intestinal microcirculation and gut permeability in acute pancreatitis: early changes and therapeutic implications
    H G Hotz
    Department of Surgery, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Berlin, Germany
    J Gastrointest Surg 2:518-25. 1998
    ....
  77. pmc Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum
    Hanno Troeger
    Campus Benjamin Franklin, Charite, Medizinische Klinik I, Gastroenterology, Infectious Diseases and Rheumatology, 12200 Berlin, Germany
    Gut 56:328-35. 2007
    ..Giardia lamblia causes infection of the small intestine, which leads to malabsorption and chronic diarrhoea...
  78. pmc Antigen transport and cytoskeletal characteristics of a distinct enterocyte population in inflammatory bowel diseases
    Sabine Kersting
    Department of Pediatrics, University of Muenster, Albert Schweitzer Str 33, 48149 Muenster, Germany
    Am J Pathol 165:425-37. 2004
    ..RACE cells are characterized by increased antigen transport to late endosomes and the trans-Golgi network, a disassembled cytoskeleton and lower concentrations of proteins that are markers of cell differentiation...