F Pragst

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. ncbi request reprint Systematic toxicological analysis by high-performance liquid chromatography with diode array detection (HPLC-DAD)
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Berlin, Germany
    Clin Chem Lab Med 42:1325-40. 2004
  2. ncbi request reprint State of the art in hair analysis for detection of drug and alcohol abuse
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Hittorfstr 18, D 14195 Berlin, Germany
    Clin Chim Acta 370:17-49. 2006
  3. doi request reprint Interpretation problems in a forensic case of abstinence determination using alcohol markers in hair
    Fritz Pragst
    University Hospital Charite, Institute of Legal Medicine, Turmstr 21, Building N, 10559 Berlin, Germany
    Forensic Sci Int 217:e4-7. 2012
  4. ncbi request reprint Wipe-test and patch-test for alcohol misuse based on the concentration ratio of fatty acid ethyl esters and squalene CFAEE/CSQ in skin surface lipids
    F Pragst
    Institute of Legal Medicine, Humboldt University, Hannoversche Strasse 6, 10115 Berlin, Germany
    Forensic Sci Int 143:77-86. 2004
  5. doi request reprint Determination of fatty acid ethyl esters (FAEE) and ethyl glucuronide (EtG) in hair: a promising way for retrospective detection of alcohol abuse during pregnancy?
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Berlin, Germany
    Ther Drug Monit 30:255-63. 2008
  6. doi request reprint Combined use of fatty acid ethyl esters and ethyl glucuronide in hair for diagnosis of alcohol abuse: interpretation and advantages
    F Pragst
    Institute of Legal Medicine, University Hospital Charite, Berlin, Germany
    Forensic Sci Int 196:101-10. 2010
  7. ncbi request reprint Poisonings with diphenhydramine--a survey of 68 clinical and 55 death cases
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Berlin, Germany
    Forensic Sci Int 161:189-97. 2006
  8. ncbi request reprint Application of solid-phase microextraction in analytical toxicology
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Hittorfstr 18, 14195 Berlin, Germany
    Anal Bioanal Chem 388:1393-414. 2007
  9. ncbi request reprint A sudden death with lung embolism after inadvertent infusion of zinc oxide shake lotion
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Berlin, Germany
    Forensic Sci Int 170:207-12. 2007
  10. ncbi request reprint Analysis of fatty acid ethyl esters in hair as possible markers of chronically elevated alcohol consumption by headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS)
    F Pragst
    Institute of Legal Medicine, Humboldt University, Hannoversche Strasse 6, D 10115 Berlin, Germany
    Forensic Sci Int 121:76-88. 2001

Collaborators

Detail Information

Publications43

  1. ncbi request reprint Systematic toxicological analysis by high-performance liquid chromatography with diode array detection (HPLC-DAD)
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Berlin, Germany
    Clin Chem Lab Med 42:1325-40. 2004
    ....
  2. ncbi request reprint State of the art in hair analysis for detection of drug and alcohol abuse
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Hittorfstr 18, D 14195 Berlin, Germany
    Clin Chim Acta 370:17-49. 2006
    ..Hair analysis for drugs is, however, not a simple routine procedure and needs substantial guidelines throughout the testing process, i.e., from sample collection to results interpretation...
  3. doi request reprint Interpretation problems in a forensic case of abstinence determination using alcohol markers in hair
    Fritz Pragst
    University Hospital Charite, Institute of Legal Medicine, Turmstr 21, Building N, 10559 Berlin, Germany
    Forensic Sci Int 217:e4-7. 2012
    ....
  4. ncbi request reprint Wipe-test and patch-test for alcohol misuse based on the concentration ratio of fatty acid ethyl esters and squalene CFAEE/CSQ in skin surface lipids
    F Pragst
    Institute of Legal Medicine, Humboldt University, Hannoversche Strasse 6, 10115 Berlin, Germany
    Forensic Sci Int 143:77-86. 2004
    ..42). It can be concluded from the results that FAEE in skin surface lipids can be used for medium-term retrospective detection of heavy drinking...
  5. doi request reprint Determination of fatty acid ethyl esters (FAEE) and ethyl glucuronide (EtG) in hair: a promising way for retrospective detection of alcohol abuse during pregnancy?
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Berlin, Germany
    Ther Drug Monit 30:255-63. 2008
    ..Until now, there has been no evaluation in context of FAS diagnosis; however, a successful application for this purpose can be expected from the good experience in driving ability examination...
  6. doi request reprint Combined use of fatty acid ethyl esters and ethyl glucuronide in hair for diagnosis of alcohol abuse: interpretation and advantages
    F Pragst
    Institute of Legal Medicine, University Hospital Charite, Berlin, Germany
    Forensic Sci Int 196:101-10. 2010
    ....
  7. ncbi request reprint Poisonings with diphenhydramine--a survey of 68 clinical and 55 death cases
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Berlin, Germany
    Forensic Sci Int 161:189-97. 2006
    ..From the results it follows that diphenhydramine is not less poisonous than other prescribed hypnotics. However, despite the hallucinogenic effects, an abuse for recreational purposes was not observed until now...
  8. ncbi request reprint Application of solid-phase microextraction in analytical toxicology
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Hittorfstr 18, 14195 Berlin, Germany
    Anal Bioanal Chem 388:1393-414. 2007
    ..However, it was shown that it also has some capacity for a general screening by direct immersion into urine samples and for pesticides and other semivolatile substance in the headspace mode...
  9. ncbi request reprint A sudden death with lung embolism after inadvertent infusion of zinc oxide shake lotion
    Fritz Pragst
    Institute of Legal Medicine, University Hospital Charite, Berlin, Germany
    Forensic Sci Int 170:207-12. 2007
    ..The results are discussed in context of pulmonary microembolism cases frequently described for drug addicts after injection of crashed talcum containing tablets...
  10. ncbi request reprint Analysis of fatty acid ethyl esters in hair as possible markers of chronically elevated alcohol consumption by headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS)
    F Pragst
    Institute of Legal Medicine, Humboldt University, Hannoversche Strasse 6, D 10115 Berlin, Germany
    Forensic Sci Int 121:76-88. 2001
    ..From the results follows that the measurement of FAEE concentrations in hair is a useful way for a retrospective detection of alcohol abuse...
  11. ncbi request reprint Squalene in hair--a natural reference substance for the improved interpretation of fatty acid ethyl ester concentrations with respect to alcohol misuse
    V Auwärter
    Institute of Legal Medicine, Humboldt University, Hannoversche Strasse 6, D 10115 Berlin, Germany
    Forensic Sci Int 145:149-59. 2004
    ..5 ng/microg). However, the relative concentration CFAEE/CSQ cannot completely replace the absolute concentration CFAEE, and both should regularly be used for an improved interpretation with respect to alcohol abuse...
  12. doi request reprint Workplace alcohol testing program by combined use of ethyl glucuronide and fatty acid ethyl esters in hair
    M Hastedt
    Institute of Legal Medicine and Forensic Sciences, Charite Universitatsmedizin Berlin, Turmstraße 21, Building N, 10559 Berlin, Germany
    Alcohol Alcohol 47:127-32. 2012
    ..The applicability of fatty acid ethyl esters (FAEEs) and ethyl glucuronide (EtG) in hair in a workplace alcohol testing program was investigated...
  13. ncbi request reprint Fatty acid ethyl esters in hair as markers of alcohol consumption. Segmental hair analysis of alcoholics, social drinkers, and teetotalers
    V Auwärter
    Institute of Legal Medicine, Humboldt University, Hannoversche Strasse 6, D 10115 Berlin, Germany
    Clin Chem 47:2114-23. 2001
    ..Fatty acid ethyl esters (FAEEs) are products of nonoxidative ethanol metabolism. After incorporation in hair, they should be suitable long-term markers of alcohol abuse...
  14. ncbi request reprint Effect of hair care and hair cosmetics on the concentrations of fatty acid ethyl esters in hair as markers of chronically elevated alcohol consumption
    Sven Hartwig
    Institute of Legal Medicine, Humboldt University, Hannoversche Strasse 6, 10115 Berlin, Germany
    Forensic Sci Int 131:90-7. 2003
    ..5% ethanol, with a deodorant and with a hair spray. As an explanation, it is assumed that FAEE are formed in the sebum glands also after regular topical application of products with a higher ethanol content...
  15. doi request reprint 11-nor-Delta9-tetrahydrocannabinol-9-carboxylic acid ethyl ester (THC-COOEt): unsuccessful search for a marker of combined cannabis and alcohol consumption
    Thomas Nadulski
    Institute of Legal Medicine, University Hospital Charite, Hittorfstr 18, 14195 Berlin, Germany
    Forensic Sci Int 196:78-84. 2010
    ..3 ng/mL in plasma and 2 pg/mg in hair in 11 samples using GC-NCI-MS and 0.2 pg/mg in one sample using GC-NCI-MS. Therefore, the use of this compound as a marker for combined cannabis and alcohol consumption could not be achieved...
  16. ncbi request reprint Shift of the high-performance liquid chromatographic retention times of metabolites in relation to the original drug on an RP8 column with acidic mobile phase
    S Herre
    Institute of Legal Medicine of the Humboldt University, Berlin, Germany
    J Chromatogr B Biomed Sci Appl 692:111-26. 1997
    ..The practical use of the T(M) values as a support to distinguish between different metabolites in the HPLC-DAD analysis of intoxications is demonstrated with cases of poisoning with diphenhydramine, propafenone and methaqualone...
  17. doi request reprint Quantification of fatty acid ethyl esters (FAEE) and ethyl glucuronide (EtG) in meconium from newborns for detection of alcohol abuse in a maternal health evaluation study
    Abdulsallam Bakdash
    Institute of Legal Medicine, University Hospital Charite, Hittorfstr 18, 14195 Berlin, Germany
    Anal Bioanal Chem 396:2469-77. 2010
    ..It is concluded that the combined use of FAEE and EtG in meconium as markers for fetal alcohol exposure essentially increases the accuracy of the interpretation and helps to avoid false positive and false-negative results...
  18. ncbi request reprint Narcotrend-assisted propofol/remifentanil anaesthesia vs clinical practice: does it make a difference?
    I Rundshagen
    Department of Anaesthesiology, Universitatsmedizin Berlin, Campus Charite Mitte, Schumannstr, 20 21, D 10117 Berlin, Germany
    Br J Anaesth 99:686-93. 2007
    ..The aim of the present study is to test the hypothesis that the intraoperative level of anaesthetic depth differs if decision-making is guided by Narcotrend monitoring or not...
  19. doi request reprint Nervus medianus evoked potentials and bispectral index during repeated transitions from consciousness to unconsciousness
    I Rundshagen
    Department of Anaesthesiology, University Hospital Charite, Berlin, Germany
    Br J Anaesth 101:366-73. 2008
    ..We investigated the relationship between median nerve somatosensory evoked potentials (SSEPs) and the bispectral index (BIS) during alternating periods of consciousness and propofol-induced unconsciousness...
  20. ncbi request reprint Oculomotor effects of delta-9-tetrahydrocannabinol in humans: implications for the functional neuroanatomy of the brain cannabinoid system
    Christoph J Ploner
    Klinik fur Neurologie, Charite, Humboldt Universitat, D 10117 Berlin, Germany
    Cereb Cortex 12:1016-23. 2002
    ..Saccadic eye movements may provide an objective measure of motor and cognitive effects of cannabinoids...
  21. ncbi request reprint Altered cell-mediated immunity and increased postoperative infection rate in long-term alcoholic patients
    Claudia D Spies
    Department of Anesthesiology and Intensive Care Medicine, Charite Universitatsmedizin Berlin, Campus Mitte, Schumannstrasse 20 21, 10098 Berlin, Germany
    Anesthesiology 100:1088-100. 2004
    ..The aim of this study was to evaluate perioperative T cell-mediated immune parameters as well as cytokine release from whole blood cells after lipopolysaccharide stimulation and its association with postoperative infections...
  22. ncbi request reprint Selectivity of substance identification by HPLC-DAD in toxicological analysis using a UV spectra library of 2682 compounds
    Matthias Herzler
    Institute of Legal Medicine, Humboldt University, Hannoversche Strasse 6, D 10115 Berlin, Germany
    J Anal Toxicol 27:233-42. 2003
    ..The practical relevance of the results for systematic toxicological analysis is demonstrated by the example of a multidrug intoxication and in the context of sample preparation methods routinely used...
  23. ncbi request reprint Fatty Acid ethyl esters in scalp, pubic, axillary, beard and body hair as markers for alcohol misuse
    Sven Hartwig
    Institute of Legal Medicine, Humboldt University, Hannoversche Strasse 6, 10115 Berlin, Germany
    Alcohol Alcohol 38:163-7. 2003
    ..This study examined the possibility of using hair samples other than scalp hair for analysis of fatty acid ethyl esters (FAEE) as markers for alcohol misuse...
  24. ncbi request reprint Analytical investigations in a death case by suffocation in an argon atmosphere
    V Auwärter
    Institute of Legal Medicine, Humboldt University, Hannoversche Strasse 6, D 10115 Berlin, Germany
    Forensic Sci Int 143:169-75. 2004
    ..They cannot be explained by a long-term inhalation of an atmosphere enriched with argon before the incident as it is likely to have occurred in this factory hall...
  25. ncbi request reprint Simple and sensitive determination of Delta(9)-tetrahydrocannabinol, cannabidiol and cannabinol in hair by combined silylation, headspace solid phase microextraction and gas chromatography-mass spectrometry
    Thomas Nadulski
    Institute of Legal Medicine, University Hospital Charite, 14195 Berlin, Hittorfstr 18, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 846:78-85. 2007
    ..12 ng/mg) using a sample amount of 30 mg. The frequently observed increase of the segmental drug concentrations from proximal to distal is explained by progressive accumulation in the hair shaft from sebum or side stream smoke...
  26. ncbi request reprint Determination of cathinone, cathine and norephedrine in hair of Yemenite khat chewers
    Frank Sporkert
    Institute of Legal Medicine, University Rostock, St Georg Street 108, 18055 Rostock, Germany
    Forensic Sci Int 133:39-46. 2003
    ..7ng/mg for CTN. A highly significant correlation was found between the self-reported data about the khat consumption habits of the volunteers (4-56h chewing per week) and the concentrations of norephedrine and norpseudoephedrine in hair...
  27. ncbi request reprint Oral fluid testing for illicit substance use in preanaesthetic care
    F Kork
    Department of Anaesthesiology and Intensive Care Medicine, Campus Virchow Clinic and Campus Charité Mitte, Charite University Medicine Berlin, Berlin, Germany
    J Int Med Res 40:194-203. 2012
    ..This prospective observational study evaluated oral fluid testing and self reporting of illicit drug use compared with confirmatory blood testing...
  28. ncbi request reprint Psychomotor dysfunction after remifentanil/propofol anaesthesia
    I Dressler
    University Hospital Charite, Department of Anaesthesiology, Berlin, Germany
    Eur J Anaesthesiol 24:347-54. 2007
    ..The hypothesis was that psychomotor performance remains reduced 24 h after anaesthesia...
  29. ncbi request reprint Cannabinoids enhance susceptibility of immature brain to ethanol neurotoxicity
    Henrik H Hansen
    Department of Pediatric Neurology and Neuroscience Research Center, Humboldt University, Berlin, Germany
    Ann Neurol 64:42-52. 2008
    ..Neurocognitive deficits have been reported in children whose mothers used marijuana during pregnancy. Maternal consumption of ethanol is known to cause serious developmental deficits..
  30. ncbi request reprint Simultaneous and sensitive analysis of THC, 11-OH-THC, THC-COOH, CBD, and CBN by GC-MS in plasma after oral application of small doses of THC and cannabis extract
    Thomas Nadulski
    Institute of Legal Medicine, University Hospital Charite, Hannoversche Strabe 6, D 10115 Berlin, Germany
    J Anal Toxicol 29:782-9. 2005
    ..Therefore, the concentration ratio 11-OH-THC/THC was discussed as a criterion for distinguishing oral from inhalative cannabis consumption...
  31. ncbi request reprint Headspace solid-phase microextraction with 1-pyrenyldiazomethane on-fibre derivatisation for analysis of fluoroacetic acid in biological samples
    Frank Sporkert
    Institute of Legal Medicine, Charite, Humboldt University, Hannoversche Strasse 6, D 10115, Berlin, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 772:45-51. 2002
    ..07 microg/ml, respectively. The method was also tested with spiked whole blood, urine, stomach contents and kidney samples. It was sufficiently reliable, reproducible and sensitive for use in routine forensic toxicology applications...
  32. ncbi request reprint Functional residual capacity measurement by heptafluoropropane in ventilated newborn lungs: in vitro and in vivo validation
    Hans Proquitte
    Clinic of Neonatology, Charite Campus Mitte, and Institute of Legal Medicine, Charite Universitatsmedizin, Berlin, Germany
    Crit Care Med 34:1789-95. 2006
    ..The aim of the present study was to validate functional residual capacity measurements by heptafluoropropane wash-in/wash-out (0.8%) during mechanical ventilation in small, surfactant-depleted lungs using a newborn piglet model...
  33. ncbi request reprint Determination of 1,1,1,2,3,3,3-heptafluoropropane (HFP) in blood by headspace gas chromatography-mass spectrometry
    Volker Auwärter
    Institute of Legal Medicine, University Hospital Charite, Hittorftrasse 18, D 14195 Berlin, Germany
    J Anal Toxicol 29:574-6. 2005
    ..A saturation concentration in blood between 2.0 and 2.5 mg/L was attained after 3 min ventilation and decreased with a half-life below 1 min after termination of the HFP supply. No relevant accumulation of HFP was observed...
  34. ncbi request reprint Application of headspace solid phase microextraction to qualitative and quantitative analysis of tobacco additives in cigarettes
    Careen Merckel
    Institute of Legal Medicine, Humboldt University, Hittorfstr 18, D 14195 Berlin, Germany
    J Chromatogr A 1116:10-9. 2006
    ..5 g NaCl or Na2SO4. Furthermore, the method was optimized for quantitative determination of 17 frequently occurring additives. The practical applicability of the method was demonstrated for cigarettes from 32 brands...
  35. ncbi request reprint Randomized, double-blind, placebo-controlled study about the effects of cannabidiol (CBD) on the pharmacokinetics of Delta9-tetrahydrocannabinol (THC) after oral application of THC verses standardized cannabis extract
    Thomas Nadulski
    Institute of Legal Medicine and Department of Psychiatry, University Hospital Charite, Berlin, Germany
    Ther Drug Monit 27:799-810. 2005
    ..Significantly higher AUC and Cmax and shorter tmax were found for females as compared with males...
  36. doi request reprint Measurement of direct ethanol metabolites in a case of a former driving under the influence (DUI) of alcohol offender, now claiming abstinence
    Friedrich M Wurst
    Psychiatric University Hospital, University of Basel, Basel, Switzerland
    Int J Legal Med 122:235-9. 2008
    ..A careful individual interpretation of the results for the different markers, however, is an absolute necessity...
  37. ncbi request reprint Concentration of fatty acid ethyl esters in hair of alcoholics: comparison to other biological state markers and self reported-ethanol intake
    Friedrich Martin Wurst
    Psychiatric University Hospital and Department Central Laboratory, University of Basel, Switzerland
    Alcohol Alcohol 39:33-8. 2004
    ..Additionally, we propose a cut-off value of FAEE to distinguish teetotallers/moderate/social drinkers from alcoholics or individuals drinking at harmful levels...
  38. pmc Chronic intake of fermented floral nectar by wild treeshrews
    Frank Wiens
    Tierphysiologie, Universitat Bayreuth, 95440 Bayreuth, Germany
    Proc Natl Acad Sci U S A 105:10426-31. 2008
    ..It is yet unclear to what extent treeshrews benefit from ingested alcohol per se and how they mitigate the risk of continuous high blood alcohol concentrations...
  39. doi request reprint Measurement of direct ethanol metabolites suggests higher rate of alcohol use among pregnant women than found with the AUDIT--a pilot study in a population-based sample of Swedish women
    Friedrich Martin Wurst
    Psychiatric University Clinic, Basel, Switzerland
    Am J Obstet Gynecol 198:407.e1-5. 2008
    ..The objective of the study was to investigate whether biomarkers of alcohol consumption would provide additional information to the use of a validated alcohol questionnaire in pregnant women...
  40. ncbi request reprint [Fatal MDMA-intoxication with postmortem diagnosis of hyperthermia]
    Ingo Wirth
    Fachhochschule der Polizei des Landes Brandenburg
    Arch Kriminol 220:141-51. 2007
    ..The problems of drug-induced hyperthermia faced by forensic pathologists and criminal investigators are discussed on the basis of a death from the autopsy material of the Berlin Institute of Legal Medicine (CCM)...
  41. ncbi request reprint World Health Organization/International Society for Biomedical Research on Alcoholism study on state and trait markers of alcohol use and dependence: back to the future
    Friedrich M Wurst
    Psychiatric University Hospital Basel, Switzerland
    Alcohol Clin Exp Res 29:1268-75. 2005
  42. ncbi request reprint Blood alcohol concentration for monitoring ethanol treatment to prevent alcohol withdrawal in the intensive care unit
    Verena Eggers
    Department of Anesthesiology and Intensive Care Medicine, University Hospital Charite, Charite Campus Mitte, Schumannstrasse 20 21, 10117 Berlin, Germany
    Intensive Care Med 28:1475-82. 2002
    ..An established protocol for the prevention of AWS is ethanol administration. The aim of this study was to evaluate possible differences in ethanol dose and levels between successfully treated patients and those who developed AWS...
  43. ncbi request reprint Emerging biomarkers: new directions and clinical applications
    Friedrich M Wurst
    Psychiatric University Hospital, Wilhelm Klein Strasse 27, CH 4025 Basel, Switzerland
    Alcohol Clin Exp Res 29:465-73. 2005
    ....