Juergen Lademann

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. ncbi Hair follicles--an efficient storage and penetration pathway for topically applied substances. Summary of recent results obtained at the Center of Experimental and Applied Cutaneous Physiology, Charité -Universitätsmedizin Berlin, Germany
    J Lademann
    Center of Experimental and Applied Cutaneous Physiology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Skin Pharmacol Physiol 21:150-5. 2008
  2. ncbi Correlation of the local distribution of topically applied substances inside the stratum corneum determined by tape-stripping to differences in bioavailability
    H J Weigmann
    Center of Experimental and Applied Cutaneous Physiology, Dermatological Clinic, , Humboldt University, Berlin, Germany
    Skin Pharmacol Appl Skin Physiol 14:98-102. 2001
  3. ncbi Lateral spreading of topically applied UV filter substances investigated by tape stripping
    U Jacobi
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charit, Humboldt University, DE-10098 Berlin, Germany
    Skin Pharmacol Physiol 17:17-22. 2004
  4. ncbi [Penetration of microparticles into human skin]
    J Lademann
    Bereich Hautphysiologie der Klinik für Dermatologie, Venerologie und Allergologie, Universitatsmedizin Berlin, Charite
    Hautarzt 55:1117-9. 2004
  5. ncbi Determination of the cuticula thickness of human and porcine hairs and their potential influence on the penetration of nanoparticles into the hair follicles
    Juergen Lademann
    Charite Universitatsmedizin Berlin, Center of Applied Cutaneous Physiology, Department of Dermatology, Berlin, Berlin D 10117, Germany
    J Biomed Opt 14:021014. 2009
  6. ncbi Penetration and storage of particles in human skin: perspectives and safety aspects
    J Lademann
    Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Eur J Pharm Biopharm 77:465-8. 2011
  7. ncbi The tape stripping procedure--evaluation of some critical parameters
    J Lademann
    Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Eur J Pharm Biopharm 72:317-23. 2009
  8. ncbi Which skin model is the most appropriate for the investigation of topically applied substances into the hair follicles?
    J Lademann
    Center of Experimental and Applied Cutaneous Physiology CCP, Department of Dermatology and Allergology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Skin Pharmacol Physiol 23:47-52. 2010
  9. ncbi Risk assessment of the application of a plasma jet in dermatology
    Juergen Lademann
    Charite Universitatsmedizin Berlin, Center of Experimental and Cutaneous Physiology, Department of Dermatology and Allergology, Chariteplatz 1, Berlin 10117, Germany
    J Biomed Opt 14:054025. 2009
  10. ncbi Comparison of two in vitro models for the analysis of follicular penetration and its prevention by barrier emulsions
    Jurgen Lademann
    Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Eur J Pharm Biopharm 72:600-4. 2009

Collaborators

Detail Information

Publications76

  1. ncbi Hair follicles--an efficient storage and penetration pathway for topically applied substances. Summary of recent results obtained at the Center of Experimental and Applied Cutaneous Physiology, Charité -Universitätsmedizin Berlin, Germany
    J Lademann
    Center of Experimental and Applied Cutaneous Physiology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Skin Pharmacol Physiol 21:150-5. 2008
    ....
  2. ncbi Correlation of the local distribution of topically applied substances inside the stratum corneum determined by tape-stripping to differences in bioavailability
    H J Weigmann
    Center of Experimental and Applied Cutaneous Physiology, Dermatological Clinic, , Humboldt University, Berlin, Germany
    Skin Pharmacol Appl Skin Physiol 14:98-102. 2001
    ..The observed low intensity blanching is correlated to the follicle orifices of the skin. After application of Temovate and Emollient, a lateral spreading of the drug must be taken into account...
  3. ncbi Lateral spreading of topically applied UV filter substances investigated by tape stripping
    U Jacobi
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charit, Humboldt University, DE-10098 Berlin, Germany
    Skin Pharmacol Physiol 17:17-22. 2004
    ..It has to be considered during drug treatment of small limited skin areas and for the interpretation of recovery rates obtained in penetration studies...
  4. ncbi [Penetration of microparticles into human skin]
    J Lademann
    Bereich Hautphysiologie der Klinik für Dermatologie, Venerologie und Allergologie, Universitatsmedizin Berlin, Charite
    Hautarzt 55:1117-9. 2004
    ..5 microm. Thus, microparticles can be used for drug delivery into the hair follicles...
  5. ncbi Determination of the cuticula thickness of human and porcine hairs and their potential influence on the penetration of nanoparticles into the hair follicles
    Juergen Lademann
    Charite Universitatsmedizin Berlin, Center of Applied Cutaneous Physiology, Department of Dermatology, Berlin, Berlin D 10117, Germany
    J Biomed Opt 14:021014. 2009
    ..530 nm), while the thickness of the cuticula obtained from porcine ear bristles were slightly thinner (approx. 320 nm)...
  6. ncbi Penetration and storage of particles in human skin: perspectives and safety aspects
    J Lademann
    Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Eur J Pharm Biopharm 77:465-8. 2011
    ..In the present review, the follicular penetration mechanisms and storage properties of particles are discussed...
  7. ncbi The tape stripping procedure--evaluation of some critical parameters
    J Lademann
    Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Eur J Pharm Biopharm 72:317-23. 2009
    ..This is used, e.g., for the determination of the homogeneity of the distribution and the ex vivo determination of a universal sun protection factor (USPF) characterizing the efficacy of sunscreens...
  8. ncbi Which skin model is the most appropriate for the investigation of topically applied substances into the hair follicles?
    J Lademann
    Center of Experimental and Applied Cutaneous Physiology CCP, Department of Dermatology and Allergology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Skin Pharmacol Physiol 23:47-52. 2010
    ....
  9. ncbi Risk assessment of the application of a plasma jet in dermatology
    Juergen Lademann
    Charite Universitatsmedizin Berlin, Center of Experimental and Cutaneous Physiology, Department of Dermatology and Allergology, Chariteplatz 1, Berlin 10117, Germany
    J Biomed Opt 14:054025. 2009
    ..Additionally, thermal damage of the tissue by the plasma can be excluded. The results of the risk assessment stimulate the in vivo application of the investigated plasma jet in the treatment of chronic wounds...
  10. ncbi Comparison of two in vitro models for the analysis of follicular penetration and its prevention by barrier emulsions
    Jurgen Lademann
    Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Eur J Pharm Biopharm 72:600-4. 2009
    ..The results give further indication that in vitro studies based on membranes of excised skin are not suitable for the investigation of the follicular penetration pathway of topically applied substances...
  11. ncbi Synergy effects between organic and inorganic UV filters in sunscreens
    J Lademann
    Medical Faculty Charité, Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, 10098 Berlin, Germany
    J Biomed Opt 10:14008. 2005
    ..In this way, more photons are absorbed, increasing the SPF. The results obtained are important for the optimization of the SPF of sunscreen formulation containing organic and inorganic UV-filter components...
  12. ncbi Hair follicles - a long-term reservoir for drug delivery
    J Lademann
    Department of Dermatology and Allergy, Center of Experimental and Applied Cutaneous Physiology CCP, Charite Universitatsmedizin Berlin, Berlin, Germany
    Skin Pharmacol Physiol 19:232-6. 2006
    ..These results underscore the importance of the hair follicle for drug delivery purposes, mainly highlighting new possibilities for the future concerning retarded delivery, application frequency, and galenic design...
  13. ncbi Optical coherent tomography for in vivo determination of changes in hair cross section and diameter during treatment with glucocorticosteroids--a simple method to screen for doping substances?
    J Lademann
    Center of Applied Cutaneous Physiology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Skin Pharmacol Physiol 21:312-7. 2008
    ....
  14. ncbi [How safe are nanoparticles?]
    J Lademann
    Center of Experimental and Applied Cutaneous Physiology, Klinik fur Dermatologie, Venerologie und Allergologie, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin
    Hautarzt 60:305-9. 2009
    ..We discuss the perspectives and risks of the topical application of nanoparticles...
  15. ncbi Optical investigations to avoid the disturbing influences of furrows and wrinkles quantifying penetration of drugs and cosmetics into the skin by tape stripping
    Juergen Lademann
    Charite University Medicine Berlin, Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, 10098 Berlin, Germany
    J Biomed Opt 10:054015. 2005
    ....
  16. ncbi Influence of nonhomogeneous distribution of topically applied UV filters on sun protection factors
    Jurgen Lademann
    Medical Faculty Charité, Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, 10098 Berlin, Germany
    J Biomed Opt 9:1358-62. 2004
    ..The direct correlation of homogeneity of distribution with the SPF opens up the possibility to increase the SPF by optimizing the formulation...
  17. ncbi Influence of microparticles on the homogeneity of distribution of topically applied substances
    J Lademann
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Charite Universitatsmedizin, Berlin, Germany
    Skin Pharmacol Physiol 21:274-82. 2008
    ....
  18. ncbi Penetration of titanium dioxide microparticles in a sunscreen formulation into the horny layer and the follicular orifice
    J Lademann
    Dermatological Clinic, Medical Faculty Charité, Humboldt University Berlin, Germany
    Skin Pharmacol Appl Skin Physiol 12:247-56. 1999
    ..The amount of TiO2 found in a given follicle was less than 1% of the applied total amount of sunscreens. A penetration of microparticles into viable skin tissue could not be detected...
  19. ncbi Investigation of the stability of coated titanium microparticles used in sunscreens
    J Lademann
    Dermatological Clinic, Medical Faculty Charité, Humboldt University Berlin, Berlin, Germany
    Skin Pharmacol Appl Skin Physiol 13:258-64. 2000
    ..Applying strong forces on the microparticles by centrifugation, exceeding by far in use conditions, resulted only in small changes in the coating...
  20. ncbi Investigation of follicular penetration of topically applied substances
    J Lademann
    Center of Experimental and Applied Cutaneous Physiology, Dermatological Clinic, Medical Faculty Charité, Humboldt University, Berlin, Germany
    Skin Pharmacol Appl Skin Physiol 14:17-22. 2001
    ..Furthermore hair growth in the selected skin areas was determined. A correlation between sebum production, hair growth activity and follicular penetration was observed...
  21. ncbi Application of optical non-invasive methods in skin physiology: a comparison of laser scanning microscopy and optical coherent tomography with histological analysis
    Juergen Lademann
    Universitätsmedizin Charité Berlin, Department of Dermatology and Allergy, Center of Experimental and Applied Cutaneous Physiology CCP, Germany
    Skin Res Technol 13:119-32. 2007
    ....
  22. ncbi Nanoparticles--an efficient carrier for drug delivery into the hair follicles
    Juergen Lademann
    Charite Universitatsmedizin Berlin, Department of Dermatology, Berlin, Germany
    Eur J Pharm Biopharm 66:159-64. 2007
    ..Taking into consideration the surface structure of the hair follicles, it was assumed that the movement of the hairs may act as a pumping mechanism pushing the nanoparticles deep into the hair follicles...
  23. ncbi Carotenoids in human skin
    Juergen Lademann
    Center of Experimental and Cutaneous Physiology CCP, Department of Dermatology and Allergology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Exp Dermatol 20:377-82. 2011
    ..The measurements are highly suited for the development of anti-ageing strategies and can be efficiently used in the medical diagnostics and therapy control...
  24. ncbi Penetration studies of topically applied substances: Optical determination of the amount of stratum corneum removed by tape stripping
    J Lademann
    Charite Universitatsmedizin Berlin, Department of Dermatology and Allergology, Center of Experimental and Applied Cutaneous Physiology CCP, D 10098 Berlin, Germany
    J Biomed Opt 11:054026. 2006
    ..Comparing the results of the measurements obtained by the corneocyte density analyzer and by uv-visible spectrometry, identical results were obtained...
  25. ncbi Follicular penetration and targeting
    Jurgen Lademann
    Department of Dermatology, Venerology and Allergology, Charite, Berlin, Germany
    J Investig Dermatol Symp Proc 10:301-3. 2005
    ..Moreover, during hair follicle cycling with initiation of anagen, newly formed vessels occur. Thus, the potential of nestin-expressing hair follicle stem cells to form neurons and blood vessels was investigated...
  26. ncbi Dermal carotenoid level and kinetics after topical and systemic administration of antioxidants: enrichment strategies in a controlled in vivo study
    Maxim E Darvin
    Charite Universitatsmedizin Berlin, Department of Dermatology, Venerology and Allergology, Berlin, Germany
    J Dermatol Sci 64:53-8. 2011
    ..The best prevention of the destructive action of free radicals in human skin is textile coverings, topical sunscreens and the development of a high antioxidative protective network...
  27. ncbi In vivo skin treatment with tissue-tolerable plasma influences skin physiology and antioxidant profile in human stratum corneum
    Joachim W Fluhr
    Universitätsmedizin Charité, Berlin, Department of Dermatology and Allergology, Center of Experimental and Cutaneous Physiology, Berlin, Germany
    Exp Dermatol 21:130-4. 2012
    ..Skin physiology parameters were influenced without damaging the skin or skin functions, indicating the safety of TTP under in vivo conditions...
  28. ncbi 40 nm, but not 750 or 1,500 nm, nanoparticles enter epidermal CD1a+ cells after transcutaneous application on human skin
    Annika Vogt
    Department of Dermatology and Allergy, Clinical Research Center for Hair and Skin Physiology, Charite Universitatsmedizin Berlin, Berlin, Germany
    J Invest Dermatol 126:1316-22. 2006
    ..We conclude that 40 nm nanoparticles, but not 750 or 1,500 nm nanoparticles, may be efficiently used to transcutaneously deliver vaccine compounds via the hair follicle into cutaneous APCs...
  29. ncbi Ex-vivo spectroscopic quantification of sunscreen efficacy: proposal of a universal sun protection factor
    Hans Juergen Weigmann
    Charite Universitatsmedizin Berlin, Department of Dermatology and Allergy, Center of Experimental and Applied Cutaneous Physiology CCP, 10098 Berlin, Germany
    J Biomed Opt 12:044013. 2007
    ..3. The USPF values objectively assess the efficacy of sunscreens considering a protection against all irradiation injuries...
  30. ncbi Kinetics of carotenoid distribution in human skin in vivo after exogenous stress: disinfectant and wIRA-induced carotenoid depletion recovers from outside to inside
    Joachim W Fluhr
    Charité Universitätsmedizin, Center of Experimental Applied Cutaneous Physiology, Department of Dermatology and Allergology, Berlin, Germany
    J Biomed Opt 16:035002. 2011
    ..We could show that the Raman microscopic spectroscopy is suited to analyze the carotenoid kinetic of stress effects and recovery...
  31. ncbi Strategy of topical vaccination with nanoparticles
    Sascha Jung
    Charite Universitatsmedizin Berlin, Department of Dermatology, Center for Experimental and Applied Cutaneous Physiology, Chariteplatz 1, Berlin D 10117, Germany
    J Biomed Opt 14:021001. 2009
    ..Penetration depth of negatively charged liposomes did not exceed 50% of the total follicle length. The fluorescence dyes were mainly detected in the hair follicle; only a small amount of dye was found in the upper parts of the epidermis...
  32. ncbi Selective follicular targeting by modification of the particle sizes
    Alexa Patzelt
    Charite Universitatsmedizin Berlin, Department of Dermatology and Allergology, Center of Experimental and Cutaneous Physiology CCP, Berlin, Germany
    J Control Release 150:45-8. 2011
    ..For the human terminal hair follicle, the situation can be expected to be similar due to a similar size ratio of the hair follicles...
  33. ncbi One-year study on the variation of carotenoid antioxidant substances in living human skin: influence of dietary supplementation and stress factors
    Maxim E Darvin
    Charite Universitatsmedizin Berlin, Department of Dermatology, Chariteplatz 1, 10117 Berlin, Germany
    J Biomed Opt 13:044028. 2008
    ..During the summer and autumn months, an increase in the level of carotenoids in the skin was measured for all volunteers. The average "seasonal increase" of the carotenoid content in the skin was determined to be 1.26-fold...
  34. ncbi Differential stripping demonstrates a significant reduction of the hair follicle reservoir in vitro compared to in vivo
    Alexa Patzelt
    Department of Dermatology, Charite Universitatsmedizin Berlin, Chariteplatz 1, Berlin, Germany
    Eur J Pharm Biopharm 70:234-8. 2008
    ..It can thus be assumed that excised skin models are suitable for penetration studies only to a limited extent, as follicular penetration is greatly diminished due to the contraction of the elastic fibres of the skin...
  35. ncbi In vivo photoprotective and anti-inflammatory effect of hyperforin is associated with high antioxidant activity in vitro and ex vivo
    Martina C Meinke
    Department of Dermatology, Venerology and Allergology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Eur J Pharm Biopharm 81:346-50. 2012
    ..Occlusive application of HP cream on non-irradiated test sites did not cause any skin irritation. Taken together, these results demonstrate that hyperforin is a powerful free radical scavenger...
  36. ncbi Follicular targeting--a promising tool in selective dermatotherapy
    Annika Vogt
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology and Allergy, Charite Universitaetsmedizin Berlin, Berlin, Germany
    J Investig Dermatol Symp Proc 10:252-5. 2005
    ..Follicular targeting offers opportunities for new developments, not only in hair therapy and in the treatment of hair follicle associated diseases but also in gene therapy and immunotherapy...
  37. ncbi Porcine ear skin: an in vitro model for human skin
    Ute Jacobi
    Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Skin Res Technol 13:19-24. 2007
    ..Porcine ear skin is used in studies of percutaneous penetration as a substitute for human skin. The structure of this tissue, including hair follicles, was studied qualitatively and quantitatively in comparison with human skin...
  38. ncbi Cutaneous concentration of lycopene correlates significantly with the roughness of the skin
    Maxim Darvin
    Charite Universitatsmedizin Berlin, Department of Dermatology, Berlin, Germany
    Eur J Pharm Biopharm 69:943-7. 2008
    ..843). These findings indicate that higher levels of antioxidants in the skin effectively lead to lower levels of skin roughness, and therefore support our hypothesis...
  39. ncbi Morphometry of human terminal and vellus hair follicles
    Annika Vogt
    Clinical Research Center for Hair and Skin Physiology, Department of Dermatology and Allergy, Charite Universitatsmedizin Berlin, Germany
    Exp Dermatol 16:946-50. 2007
    ..We provide precise information on the size and the position of key target structures in VHF and THF...
  40. ncbi Topical beta-carotene protects against infra-red-light-induced free radicals
    Maxim E Darvin
    Center of Applied Cutaneous Physiology, Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Exp Dermatol 20:125-9. 2011
    ..Resonance Raman spectroscopy can be used for the non-invasive measurements of carotenoids, which can be rated as marker substances of redox processes...
  41. ncbi Transfer of ultraviolet photon energy into fluorescent light in the visible path represents a new and efficient protection mechanism of sunscreens
    Theognosia Vergou
    A Sygros Hospital, Department of Dermatology, University of Athens, Athens, Greece
    J Biomed Opt 16:105001. 2011
    ..The transfer of UV photon energy into fluorescent light represents a new approach to increase the efficiency of sunscreens and could form the basis for a new generation of sunscreens...
  42. ncbi Influence of the absorption behavior of sunscreens in the short-wavelength UV range (UVB) and the long-wavelength UV range (UVA) on the relation of the UVB absorption to sun protection factor
    Hans Juergen Weigmann
    Charité Universitätsmedizin Berlin, Department of Dermatology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Charitéplatz 1, 10117 Berlin, Germany
    J Biomed Opt 15:055008. 2010
    ..g., antioxidants and cell-protecting agents on the efficacy of the products...
  43. ncbi Application of optical methods to characterize textile materials and their influence on the human skin
    Helene Strese
    Charité Universitätsmedizin Berlin, Department for Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin D 10117, Germany
    J Biomed Opt 16:046013. 2011
    ..Consequently, the optical method used in dermatology for the analysis of the skin can be applied to characterize and evaluate textile fabrics and their interaction with human skin in vivo...
  44. ncbi Comparison of two methods for noninvasive determination of carotenoids in human and animal skin: Raman spectroscopy versus reflection spectroscopy
    Maxim E Darvin
    Department of Dermatology, Venerology and Allergology, Center of Experimental and Cutaneous Physiology CCP, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    J Biophotonics 5:550-8. 2012
    ..A strong correlation between the carotenoid concentration determined by the RS and for the RRS system was achieved for human skin in vivo (R = 0.88) and for bovine udder skin in vitro (R = 0.81)...
  45. ncbi Two-wavelength carbon dioxide laser application for in-vitro blood glucose measurements
    Martina Meinke
    Charite Universitatsmedizin Berlin, Institut für Medizinische Physik und Lasermedizin, Berlin, Germany
    J Biomed Opt 13:014021. 2008
    ..The CO2 laser measurement is too fast to be influenced by the red blood cell sedimentation, and will be a suitable method for glucose determination in whole blood...
  46. ncbi In vivo confocal scanning laser microscopy: comparison of the reflectance and fluorescence mode by imaging human skin
    Lars E Meyer
    Charite Universitaetsmedizin, Department of Dermatology and Allergy, Center of Experimental and Applied Cutaneous Physiology CCP, Berlin, Germany
    J Biomed Opt 11:044012. 2006
    ..The comparison was drawn by scanning human skin in vivo. Representative images in varying depths were obtained and analyzed; preparation procedures are shown and discussed...
  47. ncbi Comparison of transepidermal water loss and spectroscopic absorbance to quantify changes of the stratum corneum after tape stripping
    H-J Weigmann
    Department of Dermatology, Center of Experimental and Applied Cutaneous Physiology, , Berlin, Germany
    Skin Pharmacol Physiol 18:180-5. 2005
    ..The results demonstrate the importance of tape stripping characterizing the behaviour of topically applied substances...
  48. ncbi Human percutaneous absorption of a direct hair dye comparing in vitro and in vivo results: implications for safety assessment and animal testing
    J Lademann
    Department of Dermatology and Allergy, Clinical Research Centre for Hair and Skin Physiology, Charite Universitatsmedizin, 10117 Berlin, Germany
    Food Chem Toxicol 46:2214-23. 2008
    ....
  49. ncbi Serial non-invasive measurements of dermal carotenoid concentrations in dairy cows following recovery from abomasal displacement
    Julia Klein
    Clinic for Ruminants and Swine, Faculty of Veterinary Medicine, Freie Universitat Berlin, Berlin, Germany
    PLoS ONE 7:e47706. 2012
    ..These results indicate potential applications for reflection spectroscopy for non-invasive early detection of changes in the dermal carotenoid concentrations as a reflection of the antioxidant status in an animal...
  50. ncbi [Inside-out application of ivermectin in scabies -- a hypothesis]
    Norbert Haas
    Hautklinik Charit, , Berlin
    J Dtsch Dermatol Ges 1:933-7. 2003
  51. ncbi Semiquantitative determination of the penetration of a fluorescent hydrogel formulation into the hair follicle with and without follicular closure by microparticles by means of differential stripping
    A Teichmann
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charité, Berlin, Germany
    Skin Pharmacol Physiol 19:101-5. 2006
    ..This could be very useful in skin protection to prevent the storage of harmful topically applied substances in the hair follicles, known to represent long-term reservoirs...
  52. ncbi Penetration profile of microspheres in follicular targeting of terminal hair follicles
    R Toll
    Department of Dermatology and Allergy, Centre of Experimental and Applied Cutaneous Physiology, Medical School of the Humboldt University of Berlin, Germany
    J Invest Dermatol 123:168-76. 2004
    ..Thus, the basis for follicular targeting of essential structures containing stem cells for keratinocytes, melanocytes, and mast cells has been laid...
  53. ncbi The porcine snout--an in vitro model for human lips?
    U Jacobi
    Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Exp Dermatol 14:96-102. 2005
    ..These results indicate that porcine snout can be used as an in vitro model for human lips in penetration studies. Both human and porcine tissues exhibit an efficient barrier against the penetration of topically applied substances...
  54. ncbi Comparison of four different in vitro systems to study the reservoir capacity of the stratum corneum
    U Jacobi
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charité Universitätsmedizin Berlin, 10098 Berlin, Germany
    J Control Release 103:61-71. 2005
    ..The absolute drug concentrations previously detected in human skin in vivo and in vitro were compared with the present data, affording the best accordance in the case of IP...
  55. ncbi [Follicular penetration. An important pathway for topically applied substances]
    J Lademann
    Klinik für Dermatologie und Allergologie, Medizinische Fakultät Charité, Berlin
    Hautarzt 54:321-3. 2003
    ..Topically applied substances cannot enter all follicles. We discuss the reasons for the phenomenon of open and closed hair follicles...
  56. ncbi Evaluation of the pseudo-absorption method to quantify human stratum corneum removed by tape stripping using protein absorption
    U Lindemann
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charit, Humboldt University Berlin, Berlin, Germany
    Skin Pharmacol Appl Skin Physiol 16:228-36. 2003
    ..Both the protein absorption and the absorption measured after staining correlate well with the pseudo-absorption measured at 430 nm (R(2) = 0.92 +/- 0.04 and R(2) = 0.95 +/- 0.04, respectively)...
  57. ncbi Reservoir function of the stratum corneum: development of an in vivo method to quantitatively determine the stratum corneum reservoir for topically applied substances
    A Teichmann
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charité, Berlin, Germany
    Skin Pharmacol Physiol 18:75-80. 2005
    ..The results show that the method developed is suited to quantitatively determine in vivo the SC reservoir for topically applied substances...
  58. ncbi Differential stripping: introduction of a method to show the penetration of topically applied antifungal substances into the hair follicles
    M Ossadnik
    HELIOS Klinikum Bad Saarow, Ost Brandenburgisches Tumorzentrum, Berlin, Germany
    Mycoses 50:457-62. 2007
    ..Therefore, differential stripping represents a suitable method to determine the penetration of antifungal substances into the hair follicles, which is of clinical importance for the optimization of topical antifungal therapeutics...
  59. ncbi The role of follicular penetration. A differential view
    H Schaefer
    Center of Experimental and Applied Cutaneous Physiology, Dermatological Clinic, Medical Faculty Charité, Humboldt University Berlin, Germany
    Skin Pharmacol Appl Skin Physiol 14:23-7. 2001
    ..Each of these can theoretically represent a vertical pathway for percutaneous absorption, i.e. a 'shunt'...
  60. ncbi Penetration of pollen proteins into the skin
    U Jacobi
    Department of Dermatology and Allergology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Skin Pharmacol Physiol 20:297-304. 2007
    ..To evaluate such strategies, the developed method can be used, and further studies in atopic dermatitis patients are necessary to determine whether the penetration of type I allergens is increased...
  61. ncbi Determination of the horny layer profile by tape stripping in combination with optical spectroscopy in the visible range as a prerequisite to quantify percutaneous absorption
    H Weigmann
    Charite, Universitätsklinikum der Humboldt Universität zu Berlin, Klinik fur Dermatologie, Venerologie und Allergologie mit Asthmapoliklinik, Berlin, Germany
    Skin Pharmacol Appl Skin Physiol 12:34-45. 1999
    ..This was exemplified by the determination of the penetration of chemical and physical UV filters into the horny layer...
  62. ncbi Bioavailability of clobetasol propionate-quantification of drug concentrations in the stratum corneum by dermatopharmacokinetics using tape stripping
    H Weigmann
    Charite, Universitätsklinikum der Humboldt Universität zu Berlin, Klinik fur Dermatologie, Venerologie und Allergologie mit Asthmapoliklinik, Berlin, Germany
    Skin Pharmacol Appl Skin Physiol 12:46-53. 1999
    ..In general, a lateral distribution of the applied drug must be taken into account when positioning the application areas on the forearm...
  63. ncbi The number of stratum corneum cell layers correlates with the pseudo-absorption of the corneocytes
    U Jacobi
    Department of Dermatology, Medical Faculty Charité, Berlin, Germany
    Skin Pharmacol Physiol 18:175-9. 2005
    ..In this way, the SC can be quantified using the procedure of tape stripping in combination with the spectroscopic determination of the corneocytes...
  64. ncbi Confocal laser-scanning microscopy of capillaries in normal and psoriatic skin
    Rami Archid
    Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology CCP, Charite Universitatsmedizin Berlin, Berlin, Germany
    J Biomed Opt 17:101511. 2012
    ..The diameter of the vessels could be seen as a well-quantifiable indicator for the state of psoriatic skin. CLSM could be useful for therapeutic monitoring to delay possible recurrences...
  65. ncbi Radical protection in the visible and infrared by a hyperforin-rich cream - in vivo versus ex vivo methods
    Sophia Arndt
    Department of Dermatology, Venerology and Allergology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Exp Dermatol 22:354-7. 2013
    ..In vivo, a daylong protection effect could be validated after a 4-week application time of the cream indicating that a regular application is necessary to obtain the full effect...
  66. ncbi Optical methods for noninvasive determination of carotenoids in human and animal skin
    Maxim E Darvin
    Charite Universitatsmedizin Berlin, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology CCP, Chariteplatz 1, 10117 Berlin, Germany
    J Biomed Opt 18:61230. 2013
    ..The advantages, shortcomings, and limitations of the above-mentioned optical methods are discussed...
  67. ncbi UV/VIS absorbance allows rapid, accurate, and reproducible mass determination of corneocytes removed by tape stripping
    H-J Weigmann
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charit, Humboldt University Berlin, Berlin, Germany
    Skin Pharmacol Appl Skin Physiol 16:217-27. 2003
    ..Taken together, this new method is rapid, sensitive, reproducible, and accurate. We anticipate a wide application in penetration studies as well as in dermatopharmacokinetics...
  68. ncbi Radical production by infrared A irradiation in human tissue
    M E Darvin
    Center of Applied Cutaneous Physiology CCP, Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Skin Pharmacol Physiol 23:40-6. 2010
    ..Furthermore, it was shown in the present study that the side effect in the form of radical formation could be significantly reduced by increasing the protection system of the human organism in form of the antioxidant network...
  69. ncbi [Functional food and bioavailability in the target organ skin]
    M Darwin
    Center of Experimental and Applied Cutaneous Physiology (CCP, , Venerologie und Allergologie, Charit,
    Hautarzt 57:286, 288-90. 2006
    ..On the other hand, stress, illness and UV-radiation can reduce the concentration of antioxidant substances in the skin. A high concentration of antioxidant substances is protective and associated with a reduction in skin wrinkling...
  70. ncbi The pathogenetic mechanism of anthracycline-induced palmar-plantar erythrodysesthesia
    A Martschick
    Department of Gynecology and Obstetrics, Charite Campus Virchow Klinikum, Charite Universitaetsmedizin Berlin, 10117 Berlin, Germany
    Anticancer Res 29:2307-13. 2009
    ..Unfortunately, their implementation in therapy is limited due to severe side-effects such as palmar-plantar erythrodysesthesia (PPE)...
  71. ncbi Estimation of the relative stratum corneum amount removed by tape stripping
    U Jacobi
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, , Berlin, Germany
    Skin Res Technol 11:91-6. 2005
    ....
  72. ncbi Effect of supplemented and topically applied antioxidant substances on human tissue
    M Darvin
    Center of Experimental and Applied Cutaneous Physiology, Medical Faculty Charité, Humboldt University Berlin, Berlin, Germany
    Skin Pharmacol Physiol 19:238-47. 2006
    ..Nevertheless, there are some clear tendencies concerning systemic and topical application of antioxidant substances in medicine and cosmetics, and we summarize them in the present paper...
  73. ncbi Follicular penetration of topically applied caffeine via a shampoo formulation
    N Otberg
    Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Skin Pharmacol Physiol 20:195-8. 2007
    ..Follicular drug delivery is the prerequisite for an effective treatment of androgenetic alopecia or other reasons of premature hair loss...
  74. ncbi Antifungal effect of 5-aminolevulinic acid PDT in Trichophyton rubrum
    H Kamp
    Center of Experimental and Applied Cutaneous Physiology (CCP, , Campus Mitte, Berlin, Germany
    Mycoses 48:101-7. 2005
    ..Summarizing our results, ALA PDT might be a promising approach in the reduction of T. rubrum colonization in onychomycosis...
  75. ncbi Ethnic variation in vellus hair follicle size and distribution
    S Mangelsdorf
    Department of Dermatology, Center for Experimental and Applied Cutaneous Physiology, Medical Faculty Charit, Berlin, Germany
    Skin Pharmacol Physiol 19:159-67. 2006
    ..Likewise, the development of topically applied drugs should take place under adequate consideration of ethnic differences in skin physiology...
  76. ncbi Infrared radiation-induced matrix metalloproteinase in human skin: implications for protection
    Peter Schroeder
    Cell Biology Molecular Ageing Research, Institut fuer Umweltmedizinische Forschung, Heinrich Heine University Düsseldorf gGmbH, Duesseldorf, Germany
    J Invest Dermatol 128:2491-7. 2008
    ..Thus, IRA irradiation most likely promotes premature skin aging and topical application of appropriate antioxidants represents an effective photoprotective strategy...