H Kuhn

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. ncbi request reprint 15-Lipoxygenase-2 is differentially expressed in normal and neoplastic ovary
    Jana Roffeis
    Institute of Biochemistry, University Medicine Berlin Charite, Berlin, Germany
    Eur J Cancer Prev 16:568-75. 2007
  2. ncbi request reprint Structural basis for the positional specificity of lipoxygenases
    H Kuhn
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Hessische Str 3 4, 10 115, Berlin, F R, Germany
    Prostaglandins Other Lipid Mediat 62:255-70. 2000
  3. ncbi request reprint Structural biology of mammalian lipoxygenases: enzymatic consequences of targeted alterations of the protein structure
    Hartmut Kuhn
    Institute of Biochemistry, University Medicine Berlin Charite, Monbijoustr 2, 10117 Berlin, Germany
    Biochem Biophys Res Commun 338:93-101. 2005
  4. ncbi request reprint The role of lipoxygenase-isoforms in atherogenesis
    Hartmut Kuhn
    Institute of Biochemistry, University Medicine Berlin, Berlin, Germany
    Mol Nutr Food Res 49:1014-29. 2005
  5. ncbi request reprint Amino acid differences in the deduced 5-lipoxygenase sequence of CAST atherosclerosis-resistance mice confer impaired activity when introduced into the human ortholog
    Hartmut Kuhn
    Institute of Biochemistry, Humboldt University Medical School Charité, Berlin, Germany
    Arterioscler Thromb Vasc Biol 23:1072-6. 2003
  6. ncbi request reprint Mammalian arachidonate 15-lipoxygenases structure, function, and biological implications
    Hartmut Kuhn
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Berlin, Germany
    Prostaglandins Other Lipid Mediat 68:263-90. 2002
  7. ncbi request reprint Arachidonic Acid metabolites in the cardiovascular system: the role of lipoxygenase isoforms in atherogenesis with particular emphasis on vascular remodeling
    Hartmut Kuhn
    Institute of Biochemistry, University Medicine Berlin Charite, Berlin, Germany
    J Cardiovasc Pharmacol 50:609-20. 2007
  8. ncbi request reprint Regulation of enzymatic lipid peroxidation: the interplay of peroxidizing and peroxide reducing enzymes
    Hartmut Kuhn
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Berlin, Germany
    Free Radic Biol Med 33:154-72. 2002
  9. ncbi request reprint Alterations of lipoxygenase specificity by targeted substrate modification and site-directed mutagenesis
    M Walther
    Institute of Biochemistry, , Humboldt University, Berlin, Germany
    Chem Biol 8:779-90. 2001
  10. ncbi request reprint The erythroid arachidonate 15-lipoxygenase in rat reticulocytes
    T Schewe
    Institute of Biochemistry, School of Medicine Charite, Humboldt University, Berlin, GDR
    Biomed Biochim Acta 49:S42-6. 1990

Collaborators

Detail Information

Publications74

  1. ncbi request reprint 15-Lipoxygenase-2 is differentially expressed in normal and neoplastic ovary
    Jana Roffeis
    Institute of Biochemistry, University Medicine Berlin Charite, Berlin, Germany
    Eur J Cancer Prev 16:568-75. 2007
    ..018 for metastases, respectively). These data indicate that expression of 15-lipoxygenase-2 mRNA is strongly augmented during ovarian carcinogenesis and that the enzyme may constitute a suitable candidate as a tumor marker...
  2. ncbi request reprint Structural basis for the positional specificity of lipoxygenases
    H Kuhn
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Hessische Str 3 4, 10 115, Berlin, F R, Germany
    Prostaglandins Other Lipid Mediat 62:255-70. 2000
    ....
  3. ncbi request reprint Structural biology of mammalian lipoxygenases: enzymatic consequences of targeted alterations of the protein structure
    Hartmut Kuhn
    Institute of Biochemistry, University Medicine Berlin Charite, Monbijoustr 2, 10117 Berlin, Germany
    Biochem Biophys Res Commun 338:93-101. 2005
    ..This review will briefly summarize our current understanding on the structural biology of the most important mammalian lipoxygenase isoforms and will also address selected mechanistic features of the lipoxygenase reaction...
  4. ncbi request reprint The role of lipoxygenase-isoforms in atherogenesis
    Hartmut Kuhn
    Institute of Biochemistry, University Medicine Berlin, Berlin, Germany
    Mol Nutr Food Res 49:1014-29. 2005
    ....
  5. ncbi request reprint Amino acid differences in the deduced 5-lipoxygenase sequence of CAST atherosclerosis-resistance mice confer impaired activity when introduced into the human ortholog
    Hartmut Kuhn
    Institute of Biochemistry, Humboldt University Medical School Charité, Berlin, Germany
    Arterioscler Thromb Vasc Biol 23:1072-6. 2003
    ..Comparison of 5-lipoxygenase genomic sequences of B6 and CON6 mice indicated 2 conserved amino acid exchanges in the CON6 animals, but the functional impact of these mutations has not been defined...
  6. ncbi request reprint Mammalian arachidonate 15-lipoxygenases structure, function, and biological implications
    Hartmut Kuhn
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Berlin, Germany
    Prostaglandins Other Lipid Mediat 68:263-90. 2002
    ..In addition, the major hypotheses on the physiological and patho-physiological roles of 15-LOXs will be discussed briefly...
  7. ncbi request reprint Arachidonic Acid metabolites in the cardiovascular system: the role of lipoxygenase isoforms in atherogenesis with particular emphasis on vascular remodeling
    Hartmut Kuhn
    Institute of Biochemistry, University Medicine Berlin Charite, Berlin, Germany
    J Cardiovasc Pharmacol 50:609-20. 2007
    ....
  8. ncbi request reprint Regulation of enzymatic lipid peroxidation: the interplay of peroxidizing and peroxide reducing enzymes
    Hartmut Kuhn
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Berlin, Germany
    Free Radic Biol Med 33:154-72. 2002
    ....
  9. ncbi request reprint Alterations of lipoxygenase specificity by targeted substrate modification and site-directed mutagenesis
    M Walther
    Institute of Biochemistry, , Humboldt University, Berlin, Germany
    Chem Biol 8:779-90. 2001
    ..Thus, both theories for the mechanistic basis of 5-lipoxygenation (straight and inverse substrate orientation) appear to be correct for different LOX isoforms...
  10. ncbi request reprint The erythroid arachidonate 15-lipoxygenase in rat reticulocytes
    T Schewe
    Institute of Biochemistry, School of Medicine Charite, Humboldt University, Berlin, GDR
    Biomed Biochim Acta 49:S42-6. 1990
    ..It shows immunological cross-reactivity with a polyclonal antiserum against the rabbit reticulocyte lipoxygenase. It differs from the rabbit enzyme with respect to some kinetic properties and its isoelectric point...
  11. ncbi request reprint Structural basis for lipoxygenase specificity. Conversion of the human leukocyte 5-lipoxygenase to a 15-lipoxygenating enzyme species by site-directed mutagenesis
    K Schwarz
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Hessische Strasse 3 4, D 10115 Berlin, Germany
    J Biol Chem 276:773-9. 2001
    ..These data indicate the principle possibility of interconverting 5- and 15-lipoxygenases by site-directed mutagenesis and appear to support the space-based hypothesis of positional specificity...
  12. doi request reprint Structural basis for pH-dependent alterations of reaction specificity of vertebrate lipoxygenase isoforms
    M Walther
    Institute of Biochemistry, University Medicine Berlin Charite, Germany
    Biochim Biophys Acta 1791:827-35. 2009
    ..However, we found that changes in the assay conditions (low substrate concentration) and introduction/removal of a critical histidine at the active site impact the pH-dependence of reaction specificity for some lipoxygenase isoforms...
  13. ncbi request reprint The N-terminal domain of the reticulocyte-type 15-lipoxygenase is not essential for enzymatic activity but contains determinants for membrane binding
    Matthias Walther
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Monbijoustrasse 2, D 10117 Berlin, Germany
    J Biol Chem 277:27360-6. 2002
    ....
  14. ncbi request reprint Regulation of cellular 15-lipoxygenase activity on pretranslational, translational, and posttranslational levels
    H Kuhn
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Berlin, Germany
    Lipids 34:S273-9. 1999
    ..Suicide inactivation and proteolytic breakdown may contribute to the disappearance of functional 12/15-lipoxygenase at later stages of erythropoiesis...
  15. ncbi request reprint Impact of intravenous oxygen therapy on the expression of reticulocyte-type 15-lipoxygenase in human volunteers
    P Chaitidis
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Monbijoustr 2, Berlin 10117, Germany
    Prostaglandins Leukot Essent Fatty Acids 71:271-6. 2004
    ..Since 15-lipoxygenase has been implicated in the resolving phase of acute inflammatory diseases the anti-inflammatory effects of intravenous oxygen infusion may be explained at least in part by our experimental findings...
  16. pmc In vivo action of 15-lipoxygenase in early stages of human atherogenesis
    H Kuhn
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Berlin, Germany
    J Clin Invest 99:888-93. 1997
    ..In advanced human plaques the 15-lipoxygenase may be functionally silent and specific lipoxygenase products formed in earlier stages may be decomposed or superimposed by large amounts of nonenzymatic lipid peroxidation products...
  17. ncbi request reprint Simultaneous expression of leukocyte-type 12-lipoxygenase and reticulocyte-type 15-lipoxygenase in rabbits
    M Berger
    Institute of Biochemistry, University Clinics Charite, Hessische Str 3 4, Humboldt University Berlin, Germany
    J Mol Biol 278:935-48. 1998
    ..This is of importance for the implication of both enzymes in red blood cell development and atherogenesis...
  18. ncbi request reprint Investigations into calcium-dependent membrane association of 15-lipoxygenase-1. Mechanistic roles of surface-exposed hydrophobic amino acids and calcium
    Matthias Walther
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Monbijoustrasse 2, 10117 Berlin, Germany
    J Biol Chem 279:3717-25. 2004
    ....
  19. ncbi request reprint Sequence determinants for the reaction specificity of murine (12R)-lipoxygenase: targeted substrate modification and site-directed mutagenesis
    Sunitha Meruvu
    University Medicine Berlin Charite, Monbijoustrasse 2, 10117 Berlin, Germany
    J Biol Chem 280:36633-41. 2005
    ....
  20. ncbi request reprint Affinity labeling of the rabbit 12/15-lipoxygenase using azido derivatives of arachidonic acid
    Stepan Romanov
    Institute of Biochemistry, University Medicine Berlin Charite, Monbijoustrasse 2, 10117 Berlin, Germany
    Biochemistry 45:3554-62. 2006
    ....
  21. ncbi request reprint Transient experimental anemia in cholesterol-fed rabbits induces systemic overexpression of the reticulocyte-type 15-lipoxygenase and protects from aortic lipid deposition
    F Trebus
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Monbijoustr, Berlin, Germany
    Prostaglandins Leukot Essent Fatty Acids 67:419-28. 2002
    ..These data suggest that transient experimental anemia, which is accompanied by a long-lasting overexpression of the reticulocyte-type 15-LOX protects cholesterol-fed rabbits from lipid deposition in the aortic wall...
  22. ncbi request reprint Cloning of the mouse phospholipid hydroperoxide glutathione peroxidase gene
    A Borchert
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Berlin, Germany
    FEBS Lett 446:223-7. 1999
    ..Our investigations provide useful tools for future targeted gene disruption studies...
  23. ncbi request reprint Soybean lipoxygenase-1 oxygenates synthetic polyenoic fatty acids with an altered positional specificity: evidence for inverse substrate alignment
    I Ivanov
    Institute of Biochemistry, , Humboldt University, Hessische Strasse 3-4, 10115 Berlin, Germany
    Biochemistry 40:10223-9. 2001
    ..However, simultaneous introduction of an omega-OH group and methylation of the carboxylate led to formation of significant 5-lipoxygenation products, suggesting an inverse head to tail substrate orientation...
  24. ncbi request reprint Membrane translocation of 15-lipoxygenase in hematopoietic cells is calcium-dependent and activates the oxygenase activity of the enzyme
    R Brinckmann
    Institute of Biochemistry Pathology and Dermatological Clinic, University Clinics Charite, Humboldt University, Berlin, Germany
    Blood 91:64-74. 1998
    ..This translocation, which increases the fatty acid oxygenase activity of the enzyme, is calcium-dependent, but may not require a special docking protein...
  25. ncbi request reprint Enantioselective substrate specificity of 15-lipoxygenase 1
    Igor Ivanov
    Institute of Biochemistry, University Clinics Charite, Humboldt University Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
    Biochemistry 43:15720-8. 2004
    ..This interaction, which was not observed with 16(R)-HETE, 18(R)-HETE, or 18(S)-HETE, appears to be a major reason for the high degree of enantioselectivity during lipoxygenation of 16-HETE...
  26. ncbi request reprint Structure of the promoter and complete sequence of the gene coding for the rabbit translationally controlled tumor protein (TCTP) P23
    H Thiele
    Institute of Biochemistry, University Clinics Charite, Humboldt University Berlin, Germany
    Eur J Biochem 257:62-8. 1998
    ..The results confirm that the analyzed gene is the actively transcribed TCTP gene...
  27. ncbi request reprint The diversity of the lipoxygenase family. Many sequence data but little information on biological significance
    H Kuhn
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Berlin, Germany
    FEBS Lett 449:7-11. 1999
    ..This review attempts to classify the currently known mammalian lipoxygenase isoforms and critically reviews the concepts for their biological importance...
  28. ncbi request reprint [Recommendations for prevention of roentgen contrast medium-induced nephropathy]
    M Mockel
    Charité Campus Virchow Klinikum und Campus Berlin Buch Franz Volhard Klinik Medizinische Klinik mit Schwerpunkt Kardiologie Augustenburger Platz 1 13353 Berlin
    Z Kardiol 91:719-26. 2002
  29. pmc Molecular dioxygen enters the active site of 12/15-lipoxygenase via dynamic oxygen access channels
    Jan Saam
    Institute of Biochemistry, Charite Universitatsmedizin Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
    Proc Natl Acad Sci U S A 104:13319-24. 2007
    ....
  30. ncbi request reprint Structural basis for catalytic activity and enzyme polymerization of phospholipid hydroperoxide glutathione peroxidase-4 (GPx4)
    Patrick Scheerer
    Institute of Biochemistry, University Medicine Berlin Charite, Monbijoustr 2, D 10117 Berlin, Germany
    Biochemistry 46:9041-9. 2007
    ..Site-directed mutagenesis suggested involvement of the catalytic C46 and surface exposed C10 and C66 in polymer formation. In GPx4 crystals, these residues contact adjacent protein monomers...
  31. ncbi request reprint Biologic relevance of lipoxygenase isoforms in atherogenesis
    Hartmut Kuhn
    Institute of Biochemistry, Univsersity Medicine Berlin Charite, Monbijou stra 2, 0 10115 Berlin, Germany
    Expert Rev Cardiovasc Ther 3:1099-110. 2005
    ..The available literature data will be critically reviewed and a short perspective on future developments in the field will be provided...
  32. ncbi request reprint Design and biological evaluation of photo-switchable inhibitors
    Melanie Fullbeck
    Berlin Center of Genome Based Bioinformatics, 3D Dataminig Group, Institute of Molecular Biology and Bioinformatics, University Medicine Berlin Charité CBF, Arnimallee 22, D 10117 Berlin, Germany
    Genome Inform 17:141-51. 2006
    ..Inserting hemithioindigo into this short peptide, stabilization towards proteolytic degradation is achieved. Such photo-reactive compounds might be developed as potential drugs for a great variety of diseases...
  33. doi request reprint 5-Selenization of salicylic acid derivatives yielded isoform-specific 5-lipoxygenase inhibitors
    Sun Chol Yu
    Institute of Biochemistry, University Medicine Berlin Charite, Monbijoustrasse 2, 10117 Berlin, Germany
    Org Biomol Chem 8:828-34. 2010
    ..In addition, synthesis products exhibited GPx-like activity, which was higher than that of ebselen for some derivatives...
  34. ncbi request reprint Dual role of oxygen during lipoxygenase reactions
    Igor Ivanov
    Institute of Biochemistry Humboldt University Medical School Charité, Berlin, Germany
    FEBS J 272:2523-35. 2005
    ..These findings are of biological relevance as variations of the reaction conditions may turn the lipoxygenase reaction into an efficient source of free radicals...
  35. doi request reprint Molecular enzymology of lipoxygenases
    Igor Ivanov
    Institute of Biochemistry, University Medicine Berlin Charite, Germany
    Arch Biochem Biophys 503:161-74. 2010
    ..Although the biological role of LOXs in lower organisms is far from clear, sequence data suggests that this enzyme family might have evolved shortly after the appearance of atmospheric oxygen on earth...
  36. pmc Applicability of the triad concept for the positional specificity of mammalian lipoxygenases
    Robert Vogel
    Institute of Biochemistry, University Medicine Berlin Charite, Monbijoustrasse 2, D 10117 Berlin, Germany
    J Biol Chem 285:5369-76. 2010
    ..The specificity of the platelet 12-LOX was partly explained by the triad hypothesis, but the concept was not applicable for 15-LOX2 and (12R)-LOX...
  37. ncbi request reprint Suicidal inactivation of the rabbit 15-lipoxygenase by 15S-HpETE is paralleled by covalent modification of active site peptides
    Rainer Wiesner
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Berlin, Germany
    Free Radic Biol Med 34:304-15. 2003
    ..Therefore, this protein modification contributes to suicidal inactivation...
  38. ncbi request reprint Occurrence of the erythroid cell specific arachidonate 15-lipoxygenase in human reticulocytes
    P Kroschwald
    Institute of Biochemistry, Humboldt University, Berlin, GDR
    Biochem Biophys Res Commun 160:954-60. 1989
    ..The occurrence of an erythroid cell specific lipoxygenase of similar type in reticulocytes of various mammals and man suggests the general role of this enzyme in the maturational degradation of mitochondria...
  39. ncbi request reprint Expression regulation of MAO isoforms in monocytic cells in response to Th2 cytokines
    Pavlos Chaitidis
    Institute of Biochemistry, University Medicine Berlin Charite, Berlin, Germany
    Med Sci Monit 11:BR259-65. 2005
    ..Transfection of monocytic cells (U937) with 15-LOX1 also induced MAO-A expression. These data suggested that 15-LOX1 products might play a role in the IL4-induced signaling cascade leading to expression of MAO-A in human monocytes...
  40. ncbi request reprint Expression of 12/15-lipoxygenase attenuates intracellular lipid deposition during in vitro foam cell formation
    Jutta Belkner
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Berlin, Germany
    Arterioscler Thromb Vasc Biol 25:797-802. 2005
    ..However, in several rabbit atherosclerosis models, the enzyme appears to act atheroprotective...
  41. ncbi request reprint Regulation of expression of the phospholipid hydroperoxide/sperm nucleus glutathione peroxidase gene. Tissue-specific expression pattern and identification of functional cis- and trans-regulatory elements
    Astrid Borchert
    Institute of Biochemistry, Humboldt University Medical School Charité, Monbijoustrasse 2, 10117 Berlin, Germany
    J Biol Chem 278:2571-80. 2003
    ..The decision, which GPx isoform is expressed in a given cell, appears to be made by alternative splicing of a joint primary transcript...
  42. pmc Functional characterization of cis- and trans-regulatory elements involved in expression of phospholipid hydroperoxide glutathione peroxidase
    Christoph Ufer
    Institute of Biochemistry, Humboldt University Medical School Charité, Monbijoustrasse 2, 10117 Berlin, Germany
    Nucleic Acids Res 31:4293-303. 2003
    ..The corresponding trans-regulatory proteins may contribute to differential expression regulation of the mitochondrial and cytosolic phGPx isoforms...
  43. ncbi request reprint Malaria-parasitized erythrocytes and hemozoin nonenzymatically generate large amounts of hydroxy fatty acids that inhibit monocyte functions
    Evelin Schwarzer
    Institute of Biochemistry, Humboldt University Charite, Berlin, Germany
    Blood 101:722-8. 2003
    ....
  44. ncbi request reprint The role of phospholipid hydroperoxide glutathione peroxidase isoforms in murine embryogenesis
    Astrid Borchert
    Institute of Biochemistry, University Medicine Berlin Charite, Monbijoustrasse 2, D 10117 Berlin, Germany
    J Biol Chem 281:19655-64. 2006
    ..These findings may explain in part intrauterine lethality of GPx4 knock-out mice...
  45. ncbi request reprint Induction of 15-lipoxygenase-1 impairs expression of HIV-1 receptors CD4 and CXCR4 in monocytic cells
    Pavlos Chaitidis
    Institute for Biochemistry, University Medicine Berlin Charite, Monbijoustr 2, D 10117 Berlin, Germany
    FEBS Lett 579:3691-4. 2005
    ..Since these surface proteins are essential for the binding of T-tropic HIV-1 strains expression of 15-lipoxygenase1 may confer resistance against HIV infection to monocytic cells...
  46. ncbi request reprint Age-related decrease in 15-lipoxygenase contributes to reduced vasorelaxation in rabbit aorta
    Xin Tang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 294:H679-87. 2008
    ..These data indicate that aortic 15-LO-1 expression and activity are downregulated with aging in rabbits. This decrease is paralleled by the reduced synthesis of vasoactive THETA and HEETA and aortic relaxations to acetylcholine and AA...
  47. ncbi request reprint Reticulocyte 15-lipoxygenase-I is important in acetylcholine-induced endothelium-dependent vasorelaxation in rabbit aorta
    Xin Tang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Arterioscler Thromb Vasc Biol 26:78-84. 2006
    ..These metabolites contribute to endothelium-dependent relaxations of rabbit aorta to AA and acetylcholine. We investigated the identity of rabbit aortic 15-LO and studied its importance in the regulation of vascular tone...
  48. doi request reprint Synthesis of a new seleninic acid anhydride and mechanistic studies into its glutathione peroxidase activity
    Sun Chol Yu
    Department of Pharmacy, Pyongyang Medical University, Ryonhwa dong, Central district Pyongyang DPR of Korea
    Chemistry 14:7066-71. 2008
    ..These data suggested that cycles A and B may constitute the major routes of GPx-like activity of 7, whereas cycle C may not significantly contribute to catalysis...
  49. doi request reprint Structural properties of plant and mammalian lipoxygenases. Temperature-dependent conformational alterations and membrane binding ability
    Giampiero Mei
    NAST Centre, Nanoscienze and Nanotecnologie and Strumentazione, 00133 Rome, Italy
    Biochemistry 47:9234-42. 2008
    ....
  50. ncbi request reprint Modification of HDL3 by mild oxidative stress increases ATP-binding cassette transporter 1-mediated cholesterol efflux
    Angela Pirillo
    Department of Pharmacological Sciences, Via Balzaretti, 9, 20133 Milano, Italy
    Cardiovasc Res 75:566-74. 2007
    ..In the present study we investigated the effect of a mild oxidation of HDL(3) on its function as cholesterol acceptor...
  51. ncbi request reprint A simple method for the preparation of (5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid enantiomers and the corresponding 14,15-dehydro analogues: role of the 16-hydroxy group for the lipoxygenase reaction
    Igor V Ivanov
    M V Lomonosov State Academy of Fine Chemical Technology, 117571 Pr Vernadskogo 86, Moscow, Russian Federation
    Bioorg Med Chem 10:2335-43. 2002
    ..In contrast, the product pattern of 16-HETE methyl ester oxygenation by the soybean lipoxygenase (5-lipoxygenation) may be explained by an inverse head to tail substrate orientation...
  52. ncbi request reprint Structural flexibility of the N-terminal beta-barrel domain of 15-lipoxygenase-1 probed by small angle X-ray scattering. Functional consequences for activity regulation and membrane binding
    Michal Hammel
    Institute of Biophysics and X ray Structure Research of the Austrian Academy of Sciences, Schmiedlstr 6, A 8042 Graz, Austria
    J Mol Biol 343:917-29. 2004
    ..This interdomain movement may be of functional importance for regulation of the catalytic activity and membrane binding...
  53. pmc A near null variant of 12/15-LOX encoded by a novel SNP in ALOX15 and the risk of coronary artery disease
    Themistocles L Assimes
    Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305 5406, USA
    Atherosclerosis 198:136-44. 2008
    ..We tested the hypothesis that exonic and/or promoter single nucleotide polymorphisms (SNPs) in the human 12/15-LOX gene (ALOX15) alter the risk of symptomatic coronary artery disease (CAD)...
  54. ncbi request reprint Photoactivation of an inhibitor of the 12/15-lipoxygenase pathway
    Stephan Herre
    Institute of Chemistry, Technical University Berlin, Germany
    Chembiochem 7:1089-95. 2006
    ..Since light-induced isomerization may proceed in the skin, such photoreactive compounds might be developed as potential drugs for inflammatory skin diseases...
  55. ncbi request reprint 15-Lipoxygenase-mediated modification of high-density lipoproteins impairs SR-BI- and ABCA1-dependent cholesterol efflux from macrophages
    Angela Pirillo
    Department of Pharmacological Sciences, University of Milan, Milan, Italy
    Biochim Biophys Acta 1761:292-300. 2006
    ..These results suggest that 15-lipoxygenase might induce structural alterations of HDL3 particles that impair their capability of triggering reverse cholesterol transport...
  56. ncbi request reprint Interleukin-13 upregulates vasodilatory 15-lipoxygenase eicosanoids in rabbit aorta
    Xin Tang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Arterioscler Thromb Vasc Biol 23:1768-74. 2003
    ..We tested the hypothesis that IL-13 upregulates the 15-lipoxygenase pathway in rabbit aorta by inducing 15-lipoxygenase expression, thus increasing vascular relaxation mediated by THETA and HEETA...
  57. ncbi request reprint Mechanical stretch alters alveolar type II cell mediator release toward a proinflammatory pattern
    Stefan Hammerschmidt
    Department of Respiratory Medicine and Critical Care, Universitat Leipzig, Johannisallee 32, 04103 Leipzig, Germany
    Am J Respir Cell Mol Biol 33:203-10. 2005
    ..Continuing mechanical distension of ATII cells contributes to an inflammatory response by a shift in the balance of pro- and anti-inflammatory mediators...
  58. ncbi request reprint Synthesis of (5Z,8Z,11Z,14Z)-18- and 19-azidoeicosa-5,8,11,14-tetraenoic acids and their [5,6,8,9,11,12,14,15-3H8]-analogues through a common synthetic route
    Stepan G Romanov
    M V Lomonosov State Academy of Fine Chemical Technology, Moscow, Russia
    Chem Phys Lipids 130:117-26. 2004
    ....
  59. ncbi request reprint Sequence determinants for the positional specificity of lipoxygenases
    Kristin Schwarz
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Hessische Str 3 4, D 10115 Berlin, F R Germany
    Adv Exp Med Biol 507:55-60. 2002
  60. ncbi request reprint Lipoxygenases in the cardiovascular system
    Hartmut Kuhn
    Circ Res 94:1527-9. 2004
  61. pmc Expanding expression of the 5-lipoxygenase pathway within the arterial wall during human atherogenesis
    Rainer Spanbroek
    Institute for Vascular Medicine, Friedrich Schiller University of Jena, Nordhäuserstrasse 78, 99089 Erfurt, Germany
    Proc Natl Acad Sci U S A 100:1238-43. 2003
    ..They raise the possibility that antileukotriene drugs may be an effective treatment regimen in late-stage disease...
  62. ncbi request reprint Discovery of a functional retrotransposon of the murine phospholipid hydroperoxide glutathione peroxidase: chromosomal localization and tissue-specific expression pattern
    Carolin Boschan
    Institute of Biochemistry, University Clinics Charite, Humboldt University, Monbijoustr 2, Berlin, D 10117, Germany
    Genomics 79:387-94. 2002
    ..The results are important for the design of future knockout strategies aimed at investigating the biological role of this enzyme...
  63. ncbi request reprint Influence of basic fibroblast growth factor on the proliferation of non-small cell lung cancer cell lines
    Hartmut Kuhn
    Division of Respiratory and Critical Care Medicine, Department of Medicine I, University of Leipzig, Johannisallee 32, Leipzig 04103, Germany
    Lung Cancer 44:167-74. 2004
    ..These findings suggest that bFGF stimulates the growth of tumor cells by intracrine mechanisms. Strategies to inhibit bFGF in NSCLC may therefore be a promising approach in NSCLC therapy...
  64. ncbi request reprint The 15-lipoxygenase-modified high density lipoproteins 3 fail to inhibit the TNF-alpha-induced inflammatory response in human endothelial cells
    Angela Pirillo
    Department of Pharmacological Sciences, University of Milan, Milan, Italy
    J Immunol 181:2821-30. 2008
    ..Altogether, these results demonstrate that enzymatic modification induced by 15-LO impaired the protective role of HDL(3), generating a dysfunctional lipoprotein endowed with proinflammatory characteristics...
  65. pmc Translational regulation of glutathione peroxidase 4 expression through guanine-rich sequence-binding factor 1 is essential for embryonic brain development
    Christoph Ufer
    Institute of Biochemistry, University Medicine Berlin Charite, D 10117 Berlin, F R Germany
    Genes Dev 22:1838-50. 2008
    ..These data indicate that Grsf1 up-regulates translation of GPx4 mRNA and implicate the two proteins in embryonic brain development...
  66. ncbi request reprint Influence of adenoviral vector on expression of angiogenesis regulating factors in non-small cell lung cancer cell lines
    Hartmut Kuhn
    Department of Medicine I, University of Leipzig, Johannisallee 32, 04103 Leipzig, Germany
    Cancer Immunol Immunother 51:461-6. 2002
    ..These results indicate that infection of tumor cells with the Ad vector results in an increase expression of angiogenesis-regulating factors, but does not by itself activate angiogenesis...
  67. ncbi request reprint Release of bFGF following apoptosis and necrosis in NSCLC cells: effects on chemosensitivity to cisplatin
    Hartmut Kuhn
    Department of Respiratory Medicine, University of Leipzig, Johannisallee 32, 04103 Leipzig, Germany
    Oncol Rep 14:759-62. 2005
    ..These results suggest that the release of bFGF following cell death contributes to enhanced chemosensitivity in NSCLC cell lines...
  68. ncbi request reprint Exhaled breath condensate cytokine patterns in chronic obstructive pulmonary disease
    Christian Gessner
    Department of Respiratory Medicine, University of Leipzig, Johannisallee 32, 04103 Leipzig, Germany
    Respir Med 99:1229-40. 2005
    ..01) in stable COPD (all: ex-smokers) with dose dependency for IL-8, IL-1 beta and IL-12. EBC analysis successfully characterized important differences in stable COPD compared to exacerbation or smoking and non-smoking healthy individuals...
  69. ncbi request reprint Monoamine oxidase-A modulates apoptotic cell death induced by staurosporine in human neuroblastoma cells
    Julia C Fitzgerald
    School of Biomedical and Natural Sciences, Nottingham Trent University, Clifton, Nottingham, UK
    J Neurochem 103:2189-99. 2007
    ..Therefore our data provide evidence that MAO-A, through its production of reactive oxygen species as a by-product of its catalytic activity on the mitochondrial surface, is recruited by the cell to enhance apoptotic signalling...
  70. ncbi request reprint Breath condensate nitrite correlates with hyperinflation in chronic obstructive pulmonary disease
    Christian Gessner
    Department of Respiratory Medicine, University of Leipzig, Johannisallee 32, 04103 Leipzig, Germany
    Respir Med 101:2271-8. 2007
    ..Investigations using isolated lung models and cells stretched in culture also provide insight into this relation. Exhaled breath condensate nitrite may be a biochemical indicator of pulmonary overdistension...
  71. ncbi request reprint Influence of tidal volume on pulmonary NO release, tissue lipid peroxidation and surfactant phospholipids
    Stefan Hammerschmidt
    Department of Pulmonary Medicine, Critical Care and Cardiology, Medical Department, University of Leipzig, Leipzig, Germany
    Biochim Biophys Acta 1639:17-26. 2003
    ..The increased release of NO and the accumulation of LPO products may represent early lung injury while elevated BALF-PL may reflect distension-induced surfactant secretion...
  72. ncbi request reprint Role for glutathione peroxidase-4 in brain development and neuronal apoptosis: specific induction of enzyme expression in reactive astrocytes following brain injury
    Nicolai E Savaskan
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Free Radic Biol Med 43:191-201. 2007
    ..We hypothesize that astrocytic upregulation of GPx4 in response to injury is part of a protective cascade counteracting further cell damage...
  73. ncbi request reprint Multiplex analysis of cytokines in exhaled breath condensate
    Ulrich Sack
    Institute of Clinical Immunology and Transfusion Medicine, University of Leipzig, Leipzig, Germany
    Cytometry A 69:169-72. 2006
    ..The main challenge in measurement of cytokines in EBC is the low protein content, which requires concentration steps that conflict with the need for excessive fluid required by most commonly used kits...
  74. ncbi request reprint Enhanced expression of VEGF following bFGF inhibition in non-small cell lung cancer cell lines
    Hartmut Kuhn
    Department of Respiratory Medicine, University of Leipzig, Germany
    Lung Cancer 54:149-53. 2006
    ..These results demonstrate that inhibition of bFGF appears to stimulate VEGF production in a p42/44 MAPK-dependent manner. These findings suggest that inhibition of bFGF alone is not a promising strategy to inhibit angiogenesis...