Peter-M Kloetzel

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. ncbi request reprint Antigen processing by the proteasome
    P M Kloetzel
    Institut fur Biochemie, Medical Faculty, Charite, Humboldt University, Monbijoustrasse 2, 10117 Berlin, Germany
    Nat Rev Mol Cell Biol 2:179-87. 2001
  2. pmc Mapping of O-GlcNAc sites of 20 S proteasome subunits and Hsp90 by a novel biotin-cystamine tag
    Thorsten Overath
    Institut fur Biochemie, Charite Universitatsmedizin Berlin, 13347 Berlin, Germany
    Mol Cell Proteomics 11:467-77. 2012
  3. pmc Impairment of immunoproteasome function by β5i/LMP7 subunit deficiency results in severe enterovirus myocarditis
    Elisa Opitz
    Medizinische Klinik für Kardiologie, Charite Universitatsmedizin Berlin, Berlin, Germany
    PLoS Pathog 7:e1002233. 2011
  4. pmc The 20S proteasome splicing activity discovered by SpliceMet
    Juliane Liepe
    Institut fur Biochemie, Charite, Universitatsmedizin Berlin, Berlin, Germany
    PLoS Comput Biol 6:e1000830. 2010
  5. pmc The FAT10- and ubiquitin-dependent degradation machineries exhibit common and distinct requirements for MHC class I antigen presentation
    Frederic Ebstein
    Institut fur Biochemie, Charite Universitatsmedizin Berlin, Campus CVK, Germany
    Cell Mol Life Sci 69:2443-54. 2012
  6. pmc The role of the proteasome in the generation of MHC class I ligands and immune responses
    E J A M Sijts
    Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL Utrecht, The Netherlands
    Cell Mol Life Sci 68:1491-502. 2011
  7. ncbi request reprint Proteasome and peptidase function in MHC-class-I-mediated antigen presentation
    Peter Michael Kloetzel
    Institut fur Biochemie, Charité Medizinische Fakultät der Humboldt Universität zu Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
    Curr Opin Immunol 16:76-81. 2004
  8. ncbi request reprint Generation of major histocompatibility complex class I antigens: functional interplay between proteasomes and TPPII
    Peter M Kloetzel
    Institut fur Biochemie, University Medical School Charité, Humboldt Universität Monbijoustr 2, 10117 Berlin, Germany
    Nat Immunol 5:661-9. 2004
  9. ncbi request reprint The proteasome and MHC class I antigen processing
    Peter M Kloetzel
    Institut fur Biochemie, Charite, Medizinische Fakultat der Humboldt Universitat zu Berlin, Monbijoust 2, 10117 Berlin, Germany
    Biochim Biophys Acta 1695:225-33. 2004
  10. ncbi request reprint Tumor cell lines expressing the proteasome subunit isoform LMP7E1 exhibit immunoproteasome deficiency
    Sylvia Heink
    Institute of Biochemistry, Charité Universitätsmedizin Berlin CCM, Monbijoustrasse 2, 10117 Berlin, Germany
    Cancer Res 66:649-52. 2006

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Antigen processing by the proteasome
    P M Kloetzel
    Institut fur Biochemie, Medical Faculty, Charite, Humboldt University, Monbijoustrasse 2, 10117 Berlin, Germany
    Nat Rev Mol Cell Biol 2:179-87. 2001
    ..But proteasomes--the waste-disposal units of the cell--typically do not generate peptides for antigen presentation with high efficiency. How, then, does the proteasome adapt to serve the immune system well?..
  2. pmc Mapping of O-GlcNAc sites of 20 S proteasome subunits and Hsp90 by a novel biotin-cystamine tag
    Thorsten Overath
    Institut fur Biochemie, Charite Universitatsmedizin Berlin, 13347 Berlin, Germany
    Mol Cell Proteomics 11:467-77. 2012
    ....
  3. pmc Impairment of immunoproteasome function by β5i/LMP7 subunit deficiency results in severe enterovirus myocarditis
    Elisa Opitz
    Medizinische Klinik für Kardiologie, Charite Universitatsmedizin Berlin, Berlin, Germany
    PLoS Pathog 7:e1002233. 2011
    ..This study therefore demonstrates that IP formation primarily protects the target organ of CVB3 infection from excessive inflammatory tissue damage in a virus-induced proinflammatory cytokine milieu...
  4. pmc The 20S proteasome splicing activity discovered by SpliceMet
    Juliane Liepe
    Institut fur Biochemie, Charite, Universitatsmedizin Berlin, Berlin, Germany
    PLoS Comput Biol 6:e1000830. 2010
    ....
  5. pmc The FAT10- and ubiquitin-dependent degradation machineries exhibit common and distinct requirements for MHC class I antigen presentation
    Frederic Ebstein
    Institut fur Biochemie, Charite Universitatsmedizin Berlin, Campus CVK, Germany
    Cell Mol Life Sci 69:2443-54. 2012
    ..Collectively, our data establish FAT10 modification as a distinct and alternative signal for facilitated MHC class I antigen presentation...
  6. pmc The role of the proteasome in the generation of MHC class I ligands and immune responses
    E J A M Sijts
    Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584CL Utrecht, The Netherlands
    Cell Mol Life Sci 68:1491-502. 2011
    ..We further discuss recently discovered functions of proteasomes in peptide splicing, lymphocyte survival and the regulation of cytokine production and inflammatory responses...
  7. ncbi request reprint Proteasome and peptidase function in MHC-class-I-mediated antigen presentation
    Peter Michael Kloetzel
    Institut fur Biochemie, Charité Medizinische Fakultät der Humboldt Universität zu Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
    Curr Opin Immunol 16:76-81. 2004
    ..Recent evidence suggests that tripeptidyl peptidase II (TPPII), a large peptidase with exo-and endo-proteolytic activities, is also involved in antigen processing and may generate a specific set of MHC class I epitopes...
  8. ncbi request reprint Generation of major histocompatibility complex class I antigens: functional interplay between proteasomes and TPPII
    Peter M Kloetzel
    Institut fur Biochemie, University Medical School Charité, Humboldt Universität Monbijoustr 2, 10117 Berlin, Germany
    Nat Immunol 5:661-9. 2004
    ..We therefore revisit the interferon-gamma-induced immune adaptation of the proteasome and attempt to redefine its function in connection with the emerging importance of TPPII...
  9. ncbi request reprint The proteasome and MHC class I antigen processing
    Peter M Kloetzel
    Institut fur Biochemie, Charite, Medizinische Fakultat der Humboldt Universitat zu Berlin, Monbijoust 2, 10117 Berlin, Germany
    Biochim Biophys Acta 1695:225-33. 2004
    ..Thus, a combination of several of regulatory events tunes the proteasome system for maximal efficiency in the generation of MHC class I antigens...
  10. ncbi request reprint Tumor cell lines expressing the proteasome subunit isoform LMP7E1 exhibit immunoproteasome deficiency
    Sylvia Heink
    Institute of Biochemistry, Charité Universitätsmedizin Berlin CCM, Monbijoustrasse 2, 10117 Berlin, Germany
    Cancer Res 66:649-52. 2006
    ..However, expression of LMP7E2 in these cells restores the i20S-deficient phenotype. Thus, our data describe a novel mechanism that contributes to the process of oncogenesis...
  11. pmc IFN-gamma-induced immune adaptation of the proteasome system is an accelerated and transient response
    Sylvia Heink
    Institute of Biochemistry, Charite Universitatsmedizin Berlin, 10117 Berlin, Germany
    Proc Natl Acad Sci U S A 102:9241-6. 2005
    ....
  12. ncbi request reprint A role for the proteasome regulator PA28alpha in antigen presentation
    M Groettrup
    Institute for Biochemistry, Medical Faculty Charité, Humboldt University, Berlin, Germany
    Nature 381:166-8. 1996
    ..These results demonstrate a fundamental in vivo function for PA28alpha in antigen processing...
  13. pmc PI31 is a modulator of proteasome formation and antigen processing
    Dietmar M W Zaiss
    Institute of Biochemistry, Medical Faculty Charité, Humboldt University, D 10117 Berlin, Germany
    Proc Natl Acad Sci U S A 99:14344-9. 2002
    ..Thus, PI31 represents a cellular regulator of proteasome formation and of proteasome-mediated antigen processing...
  14. pmc Hepatitis C virus mutation affects proteasomal epitope processing
    Ulrike Seifert
    Institute of Biochemistry, Charite, Humboldt University, Berlin, Germany
    J Clin Invest 114:250-9. 2004
    ....
  15. ncbi request reprint The role of the proteasome activator PA28 in MHC class I antigen processing
    Alice Sijts
    Institut fur Biochemie, Charité Medizinische Fakültät der, Humboldt Universitat zu Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
    Mol Immunol 39:165-9. 2002
    ..Our results show that PA28 selectively up-regulates the presentation of viral MHC class I epitopes and that down regulation PA28 in tumor cells results in impaired presentation of a human TRP2 tumor antigen...
  16. ncbi request reprint The murine cytomegalovirus pp89 immunodominant H-2Ld epitope is generated and translocated into the endoplasmic reticulum as an 11-mer precursor peptide
    C Knuehl
    Institute of Biochemistry, Medical Faculty, , Humboldt University, Monbijoustrasse 2a, 10117 Berlin, Germany
    J Immunol 167:1515-21. 2001
    ..In consequence, our combined proteasome/TAP assays show that the 11-mer precursor is the immunorelevant peptide product that requires N-terminal trimming in the ER for MHC class I binding...
  17. ncbi request reprint Combined effect of proteasome and calpain inhibition on cisplatin-resistant human melanoma cells
    Izabela Młynarczuk-Biały
    Institut fuer Biochemie, Charite Universitaetsmedizin Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
    Cancer Res 66:7598-605. 2006
    ..Thus, combined strategies targeting distinct proteolytic pathways may help to overcome mechanisms of drug resistance in tumor cells...
  18. ncbi request reprint Human immunodeficiency virus-1 Tat protein interacts with distinct proteasomal alpha and beta subunits
    G Sébastien Apcher
    Humboldt Universitat zu Berlin, Universitatsklinikum Charite, Institut fur Biochemie, Germany
    FEBS Lett 553:200-4. 2003
    ..This indicates that Tat can modulate or interfere with cellular proteasome function by specific interaction with distinct proteasomal subunits...
  19. ncbi request reprint An essential role for tripeptidyl peptidase in the generation of an MHC class I epitope
    Ulrike Seifert
    Institut für Biochemie Charité, Medical Faculty of the Humboldt University Berlin, Monbijoustr 2, 10117 Berlin, Germany
    Nat Immunol 4:375-9. 2003
    ..We propose that TPPII can act in combination with or independent of the proteasome system and can generate epitopes that evade generation by the proteasome-system...
  20. ncbi request reprint Circulating proteasome levels are an independent prognostic factor for survival in multiple myeloma
    Christian Jakob
    Department of Hematology and Oncology, Charite Universitatsmedizin Berlin, Germany
    Blood 109:2100-5. 2007
    ..38) survival analysis in patients with active MM. We demonstrate for the first time that increased serum proteasome concentrations correlate with advanced disease and are an independent prognostic factor in MM...
  21. ncbi request reprint Circulating proteasomes are markers of cell damage and immunologic activity in autoimmune diseases
    Karl Egerer
    Department of Rheumatology and Clinical Immunology, Institute of Biochemistry, Humboldt University of Berlin, Berlin, Germany
    J Rheumatol 29:2045-52. 2002
    ....
  22. ncbi request reprint Different proteasome subtypes in a single tissue exhibit different enzymatic properties
    B Dahlmann
    Department of Clinical Biochemistry, Deutsches Diabetes Forschungsinstitut, Dusseldorf, Germany
    J Mol Biol 303:643-53. 2000
    ..Therefore, the characteristics of proteasomes purified from tissues or cells represent the average of the different subtype activities which in turn may have different functions in vivo...
  23. ncbi request reprint Sequence information within proteasomal prosequences mediates efficient integration of beta-subunits into the 20 S proteasome complex
    M Schmidt
    Institut fur Biochemie, Medizinische Fakultät der Humboldt Universität zu Berlin Charité, Monbijoustr 2, Berlin, 10117, Germany
    J Mol Biol 288:117-28. 1999
    ..The contribution of beta5i/LMP7 on the cooperative formation of a homogeneous population of immunoproteasome is therefore most likely based on an acceleration of the beta1i/LMP2 and potentially of beta2i/MECL1 processing kinetics...
  24. ncbi request reprint Biochemical analysis of proteasomes from mouse microglia: induction of immunoproteasomes by interferon-gamma and lipopolysaccharide
    R Stohwasser
    Paul Ehrlich Center of Experimental Medicine, Humboldt University Medical School, Institute of Biochemistry, Berlin, Germany
    Glia 29:355-65. 2000
    ..These results indicate that both the 20S core protease as well as the proteasome activator PA700 are targets of modulatory subunit replacements or transient association of regulatory components in the course of microglial activation...
  25. ncbi request reprint Subtypes of 20S proteasomes from skeletal muscle
    B Dahlmann
    Institut für Biochemie Charité, Humboldt Universitat Berlin, Monbijoustr 2, 10117, Berlin, Germany
    Biochimie 83:295-9. 2001
    ..Our data show that the properties of 20S proteasomes isolated from a given tissue or cells are always the average of the properties of the whole set of proteasome subtypes...
  26. ncbi request reprint PA28alphabeta double and PA28beta single transfectant mouse B8 cell lines reveal enhanced presentation of a mouse cytomegalovirus (MCMV) pp89 MHC class I epitope
    R Stohwasser
    Institute of Biochemistry, Medical Faculty Charité, Humboldt University, Monbijoustrasse 2, 10117, Berlin, Germany
    Mol Immunol 37:13-9. 2000
    ....
  27. ncbi request reprint Characterisation of the newly identified human Ump1 homologue POMP and analysis of LMP7(beta 5i) incorporation into 20 S proteasomes
    E Witt
    Institut fur Biochemie, Charité Humboldt University Medical School, Monbijoustr 2, Berlin, 10117, Germany
    J Mol Biol 301:1-9. 2000
    ..As a result of the precursor accumulation, an increased amount of the POMP protein can be detected in these cells...
  28. ncbi request reprint The role of the ubiquitin-proteasome pathway in MHC class I antigen processing: implications for vaccine design
    A Sijts
    Institute of Biochemistry, Charite Medical School, Humboldt University Berlin, Germany
    Curr Mol Med 1:665-76. 2001
    ..The availability of such programs as well as a general insight into the proteasome mediated steps in MHC class I antigen processing provides us with a rational basis for the design of new antiviral and anticancer T cell vaccines...
  29. pmc A kinetic model of vertebrate 20S proteasome accounting for the generation of major proteolytic fragments from oligomeric peptide substrates
    H G Holzhutter
    Humboldt Universitat zu Berlin, Medizinische Fakultät Charité, Institut fur Biochemie, D 10117 Berlin, Germany
    Biophys J 79:1196-205. 2000
    ..This finding strongly supports the idea that generation of fragments with well-defined lengths is favored in that temporary immobilization of the downstream fragment after the first cleavage renders it susceptible for a second cleavage...
  30. pmc Link between organ-specific antigen processing by 20S proteasomes and CD8(+) T cell-mediated autoimmunity
    Ulrike Kuckelkorn
    Institute of Biochemistry, Charite, Humboldt University, D 10117 Berlin, Germany
    J Exp Med 195:983-90. 2002
    ..We propose tissue-specific antigen processing by 20S proteasomes as a potential mechanism to control organ-specific immune responses...
  31. ncbi request reprint Functional analysis of eukaryotic 20S proteasome nuclear localization signal
    C Knuehl
    Institut fur Biochemie, Charite, Hunboldt Universitat zu Berlin, Federal Republic of Germany
    Exp Cell Res 225:67-74. 1996
    ..Whereas the deletion of the NLS alone resulted only in a slight impairment of subunit transport into the nucleus, removal of the C-terminal 96 amino acid residues abolished nuclear translocation completely...
  32. ncbi request reprint 20S proteasome biogenesis
    E Kruger
    Institut fur Biochemie, Humboldt Universitat zu Berlin, Universitatsklinikum Charite, Monbijoustr 2, 10117, Berlin, Germany
    Biochimie 83:289-93. 2001
    ..The major localization of yeast proteasomes may point to the subcellular place of proteasome biogenesis...
  33. pmc Analysis of mammalian 20S proteasome biogenesis: the maturation of beta-subunits is an ordered two-step mechanism involving autocatalysis
    G Schmidtke
    Institut fur Biochemie, Charite, Humboldt Universitat zu Berlin, Germany
    EMBO J 15:6887-98. 1996
    ..We provide evidence that subunit processing of mammalian beta-subunits proceeds via a novel ordered two-step mechanism involving autocatalysis...
  34. ncbi request reprint Molecular cloning of the mouse proteasome subunits MC14 and MECL-1: reciprocally regulated tissue expression of interferon-gamma-modulated proteasome subunits
    R Stohwasser
    Institute for Biochemistry, Medical Faculty Charité, Humboldt University, Berlin, Germany
    Eur J Immunol 27:1182-7. 1997
    ..The impact of a regulated reciprocal tissue expression is discussed with respect to thymic selection and the induction of tolerance in potentially autoreactive T cells...
  35. ncbi request reprint Inhibition of proteasome activity induces concerted expression of proteasome genes and de novo formation of Mammalian proteasomes
    Silke Meiners
    Humboldt Universitat zu Berlin, Universitatsklinikum Charite, Institut fur Biochemie, Monbijoustrasse 2, 10117 Berlin, Germany
    J Biol Chem 278:21517-25. 2003
    ....
  36. ncbi request reprint A second gene encoding the mouse proteasome activator PA28beta subunit is part of a LINE1 element and is driven by a LINE1 promoter
    D M Zaiss
    Institute of Biochemistry Charité, Humboldt University Medical School, Humboldt University, Monbijoustrasse 2, Berlin, 10117, Germany
    J Mol Biol 287:829-35. 1999
    ....
  37. pmc Subcellular distribution of proteasomes implicates a major location of protein degradation in the nuclear envelope-ER network in yeast
    C Enenkel
    Institut fur Biochemie, Humboldt Universitat, Universitatsklinikum Charite, Monbijoustrasse 2, D 10117 Berlin, Germany
    EMBO J 17:6144-54. 1998
    ..A major location of proteasomal peptide cleavage activity was visualized in the NE-ER network, indicating that proteasomal degradation takes place mainly in this subcellular compartment in yeast...
  38. ncbi request reprint The RTP site shared by the HIV-1 Tat protein and the 11S regulator subunit alpha is crucial for their effects on proteasome function including antigen processing
    Xiaohua Huang
    Division of Molecular Biology, Department of Surgery, Medical Faculty Charité, Humboldt University, Monbijoustr 2a, Berlin, Germany
    J Mol Biol 323:771-82. 2002
    ..In contrast, the Tat peptide 37-72 with mutations of amino acid residues Lys51, Arg52 and Asp67 to Ala was not able to reduce antigen presentation...
  39. ncbi request reprint A comprehensive view on proteasomal sequences: implications for the evolution of the proteasome
    Christoph Gille
    Institute of Biochemistry, Medical Faculty Charité, Humboldt University, D 10117, Berlin, Germany
    J Mol Biol 326:1437-48. 2003
    ..The structure-based multiple proteasomal alignment of 433 sequences from 143 organisms can be downloaded from the URL dagger and will be updated regularly...