F Kern

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. ncbi request reprint Cytomegalovirus (CMV) phosphoprotein 65 makes a large contribution to shaping the T cell repertoire in CMV-exposed individuals
    Florian Kern
    Institut für Medizinische Immunologie der Charité, Campus Mitte, 10098 Berlin, Germany
    J Infect Dis 185:1709-16. 2002
  2. pmc Protection from cytomegalovirus after transplantation is correlated with immediate early 1-specific CD8 T cells
    Torsten Bunde
    Institut fur Medizinische Immunologie, Charite, Universitatsmedizin Berlin, Campus Mitte, 10098 Berlin, Germany
    J Exp Med 201:1031-6. 2005
  3. ncbi request reprint HLA type-independent generation of antigen-specific T cells for adoptive immunotherapy
    Markus H Hammer
    Department of Nephrology and Internal Intensive Care, Universitatsmedizin Berlin, Berlin, Germany
    Eur J Immunol 35:2250-8. 2005
  4. pmc Target structures of the CD8(+)-T-cell response to human cytomegalovirus: the 72-kilodalton major immediate-early protein revisited
    F Kern
    Institut fur Medizinische Immunologie, Medizinische Fakultät der Humboldt Universität zu Berlin Charité, 10098 Berlin, Germany
    J Virol 73:8179-84. 1999
  5. ncbi request reprint Distribution of human CMV-specific memory T cells among the CD8pos. subsets defined by CD57, CD27, and CD45 isoforms
    F Kern
    Institut für Medizinische Immunologie Charité Campus Mitte, Humboldt Universitat zu Berlin, Berlin, Germany
    Eur J Immunol 29:2908-15. 1999
  6. ncbi request reprint Measurement of anti-human cytomegalovirus T cell reactivity in transplant recipients and its potential clinical use: a mini-review
    F Kern
    Institut fur Medizinische Immunologie, Charité Medizinische Fakultät der Humboldt Universität zu Berlin, Berlin, Deutschland
    Intervirology 42:322-4. 1999
  7. ncbi request reprint Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides
    F Kern
    Institut fur Medizinische Immunologie, Charite, Berlin, Germany
    Eur J Immunol 30:1676-82. 2000
  8. ncbi request reprint Measuring Ag-specific immune responses: understanding immunopathogenesis and improving diagnostics in infectious disease, autoimmunity and cancer
    Florian Kern
    Institut fur Medizinische Immunologie, Charite Universitatsmedizin Berlin, Campus Mitte, 10098 Berlin, Germany
    Trends Immunol 26:477-84. 2005
  9. ncbi request reprint Immunomodulatory therapies: challenges of individualized therapy strategies
    H D Volk
    Institute for Medical Immunology, Charite University Medicine Berlin, Chariteplatz 1, 10117 Berlin, Germany
    Ernst Schering Found Symp Proc . 2006
  10. ncbi request reprint A protocol for combining proliferation, tetramer staining and intracellular cytokine detection for the flow-cytometric analysis of antigen specific T-cells
    L Tesfa
    Institut fur Medizinische Immunologie, Berliner Hochschulmedizin Charite, Charite Campus Mitte, Berlin, Germany
    J Biol Regul Homeost Agents 17:366-70. 2003

Collaborators

  • P Reinke
  • H D Volk
  • L Tesfa
  • Jan W Gratama
  • Mario Roederer
  • Louis J Picker
  • B Sawitzki
  • Andreas Moosmann
  • S M Barry
  • M Noutsias
  • Ulrich Keilholtz
  • George Janossy
  • Constanze Schonemann
  • Ingmar A F M Heijnen
  • Hans Jochem Kolb
  • F Zipp
  • Nancy A Hosken
  • Marc Bonneville
  • Bodo Hoffmeister
  • Mathias Streitz
  • Georgy Cherepnev
  • Bernhard Ay
  • Torsten Bunde
  • Stephan Fuhrmann
  • Felix Kiecker
  • Alexander Kirchner
  • Markus H Hammer
  • Christian Pelte
  • Rudolf Volkmer
  • Giuseppina Li Pira
  • Anne Letsch
  • Gordon Brestrich
  • Roland Hetzer
  • Rudolf Volkmer-Engert
  • Wolfgang Hammerschmidt
  • Andrew W Sylwester
  • Martina Wiesner
  • Jan D Lunemann
  • Nora Laske
  • Heinz Peter Schultheiss
  • Dirk Lassner
  • Georgy Cherepnev-G
  • Raskit Lachmann
  • Andrea Block
  • Katja Kotsch
  • Michael Hummel
  • Hans B Lehmkuhl
  • Pauline Meij
  • Katrin Klippert
  • Uwe Kuhl
  • Maria Rohde
  • Andreas Schlosser
  • Il Kang Na
  • Carmen Scheibenbogen
  • Anne Marie Asemissen
  • Ulrich Keilholz
  • Carsten C Mahrenholz
  • Maren Knoedler
  • Eckhard Thiel
  • Prisca Boisguerin
  • Fabrizio Manca
  • Michael Loesch
  • Sebastian D Schuck
  • Kenneth H Grabstein
  • Paul R Sleath
  • John B Edgar
  • Caroline Zentz
  • Hans Lehmkuhl
  • Jay A Nelson
  • Bridget L Mitchell
  • Mark Cobbold
  • Susanna Proesch
  • Dirk Habedank
  • Cara Taormina
  • Nina Babel
  • Reinhard Zeidler
  • Franziska Ruchti
  • Cliona M Rooney
  • Sonja Meyer
  • Alexa Mittenzweig
  • Ruud van der Zee
  • Uwe Wendling
  • Yanjun Wang
  • Constanze Schoenemann
  • Sandra Rückert
  • Ina Maria Rudawsky
  • Ulrich Wahn
  • Volker Schuster
  • Renate Nickel
  • Elham Khatamzas

Detail Information

Publications30

  1. ncbi request reprint Cytomegalovirus (CMV) phosphoprotein 65 makes a large contribution to shaping the T cell repertoire in CMV-exposed individuals
    Florian Kern
    Institut für Medizinische Immunologie der Charité, Campus Mitte, 10098 Berlin, Germany
    J Infect Dis 185:1709-16. 2002
    ..Several new T cell epitopes were identified...
  2. pmc Protection from cytomegalovirus after transplantation is correlated with immediate early 1-specific CD8 T cells
    Torsten Bunde
    Institut fur Medizinische Immunologie, Charite, Universitatsmedizin Berlin, Campus Mitte, 10098 Berlin, Germany
    J Exp Med 201:1031-6. 2005
    ..In conclusion, high frequencies of IE-1 but not pp65-specific CD8 T cells correlate with protection from CMV disease. These results have important implications for monitoring T cell responses, adoptive cell therapy, and vaccine design...
  3. ncbi request reprint HLA type-independent generation of antigen-specific T cells for adoptive immunotherapy
    Markus H Hammer
    Department of Nephrology and Internal Intensive Care, Universitatsmedizin Berlin, Berlin, Germany
    Eur J Immunol 35:2250-8. 2005
    ..The protocol combines the biosafety of peptide-based approaches with HLA type independence and may help to advance adoptive immunotherapy in the future...
  4. pmc Target structures of the CD8(+)-T-cell response to human cytomegalovirus: the 72-kilodalton major immediate-early protein revisited
    F Kern
    Institut fur Medizinische Immunologie, Medizinische Fakultät der Humboldt Universität zu Berlin Charité, 10098 Berlin, Germany
    J Virol 73:8179-84. 1999
    ..In summary, our results suggest that IE-1 is far more important as a CD8(+)-T-cell target than current opinion suggests...
  5. ncbi request reprint Distribution of human CMV-specific memory T cells among the CD8pos. subsets defined by CD57, CD27, and CD45 isoforms
    F Kern
    Institut für Medizinische Immunologie Charité Campus Mitte, Humboldt Universitat zu Berlin, Berlin, Germany
    Eur J Immunol 29:2908-15. 1999
    ..This subpopulation likely includes the fully differentiated memory cells responsible for the long-term immune defense against HCMV reactivation...
  6. ncbi request reprint Measurement of anti-human cytomegalovirus T cell reactivity in transplant recipients and its potential clinical use: a mini-review
    F Kern
    Institut fur Medizinische Immunologie, Charité Medizinische Fakultät der Humboldt Universität zu Berlin, Berlin, Deutschland
    Intervirology 42:322-4. 1999
    ..We also discuss a number of preconditions for integrating such testing into the clinical routine...
  7. ncbi request reprint Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides
    F Kern
    Institut fur Medizinische Immunologie, Charite, Berlin, Germany
    Eur J Immunol 30:1676-82. 2000
    ..Similar pools may be applied to examine the role of proteins from other pathogens, in autoimmune disease or following vaccination...
  8. ncbi request reprint Measuring Ag-specific immune responses: understanding immunopathogenesis and improving diagnostics in infectious disease, autoimmunity and cancer
    Florian Kern
    Institut fur Medizinische Immunologie, Charite Universitatsmedizin Berlin, Campus Mitte, 10098 Berlin, Germany
    Trends Immunol 26:477-84. 2005
    ....
  9. ncbi request reprint Immunomodulatory therapies: challenges of individualized therapy strategies
    H D Volk
    Institute for Medical Immunology, Charite University Medicine Berlin, Chariteplatz 1, 10117 Berlin, Germany
    Ernst Schering Found Symp Proc . 2006
    ..Herein we report on new biomarkerguided studies in the immunosuppressive treatment of transplant patients and patients with autoimmune disease and we discuss its benefits and pitfalls...
  10. ncbi request reprint A protocol for combining proliferation, tetramer staining and intracellular cytokine detection for the flow-cytometric analysis of antigen specific T-cells
    L Tesfa
    Institut fur Medizinische Immunologie, Berliner Hochschulmedizin Charite, Charite Campus Mitte, Berlin, Germany
    J Biol Regul Homeost Agents 17:366-70. 2003
    ....
  11. ncbi request reprint Selection of CMV-specific CD8+ and CD4+ T cells by mini-EBV-transformed B cell lines
    Martina Wiesner
    Department of Gene Vectors, GSF National Research Center for Environment and Health, Munich, Germany
    Eur J Immunol 35:2110-21. 2005
    ..Using a peptide library covering the pp65 sequence for further analysis of T cell clones, we identified new pp65 CD8(+) and CD4(+) T cell epitopes...
  12. pmc Broadly targeted human cytomegalovirus-specific CD4+ and CD8+ T cells dominate the memory compartments of exposed subjects
    Andrew W Sylwester
    Vaccine and Gene Therapy Institute, Department of Pathology, Oregon Health and Science University, Beaverton, 97006, USA
    J Exp Med 202:673-85. 2005
    ..These data provide the first glimpse of the total human T cell response to a complex infectious agent and will provide insight into the rules governing immunodominance and cross-reactivity in complex viral infections of humans...
  13. ncbi request reprint Sorting and pooling strategy: a novel tool to map a virus proteome for CD8 T-cell epitopes
    Bernhard Ay
    Institut für Medizinische Immunulogie, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    Biopolymers 88:64-75. 2007
    ..So far, 9069 of these peptides have been tested in an ex vivo T-cell stimulation assay. As well as confirming a number of previously known epitopes, we identified several new ones...
  14. ncbi request reprint Measurement of antigen specific immune responses: 2006 update
    Giuseppina Li Pira
    Viral Immunology, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
    Cytometry B Clin Cytom 72:77-85. 2007
    ....
  15. ncbi request reprint CMV-specific central memory T cells reside in bone marrow
    Anne Letsch
    Department of Hematology, Oncology, Charite, Berlin, Germany
    Eur J Immunol 37:3063-8. 2007
    ..CMV specific BM-derived T(CM) might be a valuable source for generating T cells for adoptive transfer...
  16. ncbi request reprint Use of peptides and peptide libraries as T-cell stimulants in flow cytometric studies
    Georgy Cherepnev
    Institute for Medical Immunology, Charite, Universitatsmedizin Berlin, Campus Charite Mitte, Berlin 10117, Germany
    Methods Cell Biol 75:453-79. 2004
  17. ncbi request reprint Enumeration of antigen-specific CD8+ T lymphocytes by single-platform, HLA tetramer-based flow cytometry: a European multicenter evaluation
    Ingmar A F M Heijnen
    Division of Immunology, Department of Laboratory Medicine, Kantonsspital Aarau, Aarau, Switzerland
    Cytometry B Clin Cytom 62:1-13. 2004
    ..Hence, the European Working Group on Clinical Cell Analysis set out to develop and evaluate a single-platform tetramer-based method that used cytomegalovirus (CMV) as the antigenic model...
  18. ncbi request reprint Comparison of proliferation and rapid cytokine induction assays for flow cytometric T-cell epitope mapping
    Lydia Tesfa
    Institute for Medical Immunology, Universitätsklinikum Charité der Humboldt Universität zu Berlin, Berlin, Germany
    Cytometry A 52:36-45. 2003
    ..We investigated whether the same epitopes could be identified by proliferation studies...
  19. doi request reprint Dissection of the CMV specific T-cell response is required for optimized cardiac transplant monitoring
    Alexander Kirchner
    Institut fur Medizinische Immunologie, Charite, Berlin, Germany
    J Med Virol 80:1604-14. 2008
    ..These results should be used to inform the design of clinically applicable and diagnostically useful approaches to monitoring CMV specific responses in heart transplant recipients...
  20. ncbi request reprint No difference in type 1 T-cell immune responses to human cytomegalovirus antigens between atopic children and nonatopic children
    Nora Laske
    J Allergy Clin Immunol 112:210-2. 2003
  21. ncbi request reprint Detection of antigen-specific T cells by cytokine flow cytometry: the use of whole blood may underestimate frequencies
    Bodo Hoffmeister
    Institut fur Medizinische Immunologie, Medizinische Fakultat der Humboldt Universitat zu Berlin, Charite, Berlin, Germany
    Eur J Immunol 33:3484-92. 2003
    ..In several instances, responses identified with PBMC were not at all detected with whole blood. In summary, studies using whole blood in this type of assay are likely to underestimate the frequencies of antigen-specific T cells...
  22. ncbi request reprint Random screening of proteins for HLA-A*0201-binding nine-amino acid peptides is not sufficient for identifying CD8 T cell epitopes recognized in the context of HLA-A*0201
    Christian Pelte
    Institut für Medizinische Immunologie der Charité, Humboldt Universität zu Berlin Charité, Campus Charite Mitte, Berlin, Germany
    J Immunol 172:6783-9. 2004
    ..We conclude that MHC/peptide binding predictions are helpful for locating epitopes in known target proteins but not necessarily for screening epitopes in proteins not known to be T cell targets...
  23. ncbi request reprint Analysis of antigen-specific T-cell responses with synthetic peptides--what kind of peptide for which purpose?
    Felix Kiecker
    Abteilung Klinische Immunologie, Institut für Medizinische Immunologie der Charité, Humboldt Universität zu Berlin Charité, Campus Charite Mitte, Berlin, Germany
    Hum Immunol 65:523-36. 2004
    ..These parameters may be modified subject to the purpose of a study...
  24. ncbi request reprint Confirmation of Mycobacterium tuberculosis infection by flow cytometry after ex vivo incubation of peripheral blood T cells with an ESAT-6-derived peptide pool
    Lydia Tesfa
    Institut für Medizinische Immunologie der Charité, Humboldt Universität zu Berlin Charité, Campus Charite Mitte, Berlin, Germany
    Cytometry B Clin Cytom 60:47-53. 2004
    ..Established procedures measure secreted T-cell cytokines following whole blood stimulation with recombinant ESAT-6 protein or use Elispot as a read-out...
  25. ncbi request reprint Cross-sectional and longitudinal analysis of myelin-reactive T cells in patients with multiple sclerosis
    Jan D Lunemann
    Institute of Neuroimmunology, Neuroscience Research Center, Charite, 10098, Berlin, Germany
    J Neurol 251:1111-20. 2004
    ..Our data provide new perspectives in detecting pathogenetically relevant T cells, but clearly demonstrate the different conclusions which must be drawn from various approaches concerning the quantification of autoreactive T cells...
  26. ncbi request reprint Evaluation of the frequency of virus-specific CD8+ T cells by cytokine flow cytometry
    Bodo Hoffmeister
    Institute of Medical Immunology, Charite, Humboldt University, Berlin, Germany
    Methods Mol Biol 215:59-69. 2003
  27. ncbi request reprint The role of flow cytometry in the interferon-gamma-based diagnosis of active tuberculosis and its coinfection with HIV-1--A technically oriented review
    George Janossy
    Department of Immunology, Royal Free and University College Medical School, London, United Kingdom
    Cytometry B Clin Cytom 74:S141-51. 2008
    ..In active TB, but not in BCG vaccinees, TB-specific memory CD4+ T cells can be found in blood that are dominantly CD27-negative and probably lung seeking and can be diagnostically useful...
  28. doi request reprint T cell response to the cytomegalovirus major capsid protein (UL86) is dominated by helper cells with a large polyfunctional component and diverse epitope recognition
    Stephan Fuhrmann
    Institut fur Medizinische Immunologie, Charite Universitatsmedizin Berlin, Germany
    J Infect Dis 197:1455-8. 2008
    ..A total of 8 CD4 T cell epitopes were identified. In contrast, CD8 T cell responses to UL86 were rare and small. UL86 is of interest for monitoring the response to CMV...
  29. pmc NS1 specific CD8+ T-cells with effector function and TRBV11 dominance in a patient with parvovirus B19 associated inflammatory cardiomyopathy
    Mathias Streitz
    Institute of Medical Immunology, Charite Universitatsmedizin Berlin, Berlin, Germany
    PLoS ONE 3:e2361. 2008
    ..Despite the importance of T-cells in antiviral defense, little is known about the role of B19V specific T-cells in this entity...
  30. ncbi request reprint Mapping T cell epitopes by flow cytometry
    Bodo Hoffmeister
    Department of Medical Immunology, Charite, Humboldt Universitat zu Berlin, Schumannstrasse 21, 10117 Berlin, Germany
    Methods 29:270-81. 2003
    ..This article includes information on peptide configuration, a section on the design and efficient application of peptide pools, and working laboratory protocols for both assays...