Genomes and Genes
Affiliation: Humboldt University
- Genetic variation of TLR4 influences immunoendocrine stress response: an observational study in cardiac surgical patientsAlexander Koch
Clinic of Anaesthesiology, Intensive Care Medicine and Pain Therapy, JW Goethe University Hospital, Theodor Stern Kai 7, Frankfurt am Main 60590, Germany
Crit Care 15:R109. 2011..To investigate the link to systemic inflammation in this context, we additionally measured 10 different cytokines in the serum...
- Influence of genetic variations in TLR4 and TIRAP/Mal on the course of sepsis and pneumonia and cytokine release: an observational study in three cohortsOliver Kumpf
Department of Anesthesiology, Intensive Care Medicine and Pain Management, HANSE Klinikum Stralsund, Grosse Parower Strasse 47 53, Stralsund 18435, Germany
Crit Care 14:R103. 2010....
- Acute-phase concentrations of lipopolysaccharide (LPS)-binding protein inhibit innate immune cell activation by different LPS chemotypes via different mechanismsLutz Hamann
Institute for Microbiology and Hygiene, Charite University Medical Center, Humboldt University Berlin, Dorotheenstrasse 96, 10117 Berlin, Germany
Infect Immun 73:193-200. 2005..A complete understanding of the host's mechanisms to modulate the proinflammatory effects of LPS will most likely help in the understanding of inflammation and infection and may lead to novel therapeutic intervention strategies...
- A coding mutation within the first exon of the human MD-2 gene results in decreased lipopolysaccharide-induced signalingL Hamann
Institute for Microbiology and Hygiene, Charite Medical Center, Humboldt University, Berlin, Germany
Genes Immun 5:283-8. 2004..Genotyping of a further 41 patients by a newly developed Lightcycler/FRET method failed to detect any additional polymorphism carriers, indicating that this is a rare mutation...
- Rapid and inexpensive real-time PCR for genotyping functional polymorphisms within the Toll-like receptor -2, -4, and -9 genesLutz Hamann
Institute for Microbiology and Hygiene, Charite Medical Center, Humboldt University, Dorotheenstrasse, Berlin 96 10117, Germany
J Immunol Methods 285:281-91. 2004..In addition, we provide protocols employing a standard Taq polymerase in order to reduce substantially the costs for real-time PCR...
- Toll-like receptor (TLR)-9 promotor polymorphisms and atherosclerosisLutz Hamann
Institute for Microbiology and Hygiene, Charite University Medical Center, Medicine, Dorotheenstrasse 96, 10117 Berlin, Germany
Clin Chim Acta 364:303-7. 2006..Specially, C. pneumonia and CMV as intracellular pathogens may be potent trigger of TLR-9 signaling. Therefore, we investigated whether the two common promotor polymorphisms of the TLR-9 gene are correlated with atherogenesis...
- Low frequency of the TIRAP S180L polymorphism in Africa, and its potential role in malaria, sepsis, and leprosyLutz Hamann
Institute of Medical Microbiology and Hygiene, Charite University Medical Center Berlin, Germany
BMC Med Genet 10:65. 2009....
- The toll-like receptor 1 variant S248N influences placental malariaLutz Hamann
Institute for Microbiology and Hygiene, Charite University Medical Center, Dorotheenstrasse 96, 10117 Berlin, Germany
Infect Genet Evol 10:785-9. 2010..These data support that TLR-1 is involved in the recognition of P. falciparum and indicate its role in susceptibility to and manifestation of malaria in pregnancy...
- Inhibition of LPS-induced activation of alveolar macrophages by high concentrations of LPS-binding proteinLutz Hamann
Department of Immunology and Cell Biology, Center for Medicine and Bioscience, Borstel, Germany
Biochem Biophys Res Commun 295:553-60. 2002..In contrast, the lung epithelial cell line A549 responded to high concentrations of LBP by an enhanced LPS uptake which did not result in cellular activation, suggesting an anti-inflammatory function of these cells by clearing LPS...
- Binding of lipopolysaccharide (LPS) to CHO cells does not correlate with LPS-induced NF-kappaB activationL Hamann
Research Center Borstel Center for Medicine and Bioscience, Borstel, Germany
Eur J Immunol 30:211-6. 2000..We conclude that CD14 is more than a catalyst for LPS binding: it seems to be directly involved in LPS signaling and thus appears to be an essential part of the signaling complex...
- Association of the toll-like receptor 2 A-16934T promoter polymorphism with severe atopic dermatitisD Y Oh
Institut für Mikrobiologie und Hygiene, Allergie Centrum Charité, CCM, Charite Universitatsmedizin Berlin, Berlin, Germany
Allergy 64:1608-15. 2009..A genetically determined defective function of pattern recognition receptors such as toll-like receptors (TLRs) has been proposed as a candidate mechanism in the pathogenesis of AD...
- Association of vitamin D receptor gene polymorphisms with severe atopic dermatitis in adultsG Heine
Klinik fur Dermatologie, Venerologie und Allergologie, Allergie Centrum Charité, CCM, Charite Universitatsmedizin Berlin, Berlin, Germany
Br J Dermatol 168:855-8. 2013..Vitamin D mediates immunomodulatory functions, and beneficial functions in allergic diseases have been suggested. Vitamin D receptor gene (VDR) polymorphisms are known but have not been studied in patients with atopic dermatitis (AD)...
- Decay-accelerating factor (DAF/CD55) is a functional active element of the LPS receptor complexH Heine
Center for Medicine and Biosciences, Research Center Borstel, Borstel, Germany
J Endotoxin Res 7:227-31. 2001..Our results demonstrate a new functional role of CD55 as a molecule able to mediate LPS-induced activation of cells that may be part of a multimeric LPS receptor complex...
- Ventilation-induced chemokine and cytokine release is associated with activation of nuclear factor-kappaB and is blocked by steroidsH D Held
Divisions of Pulmonary Pharmacology and Cellular Immunology, Research Center Borstel, Borstel, Germany
Am J Respir Crit Care Med 163:711-6. 2001..Our data further suggest that steroids might be considered as a subsidiary treatment during artificial mechanical ventilation...
- Accumulation of inhibitory kappaB-alpha as a mechanism contributing to the anti-inflammatory effects of surfactant protein-AYingda Wu
Department of Immunochemistry and Biochemical Microbiology, Research Center Borstel, Parkallee 22, 23845 Borstel, Germany
Am J Respir Cell Mol Biol 31:587-94. 2004..The data suggest that anti-inflammatory effects of SP-A on LPS-challenged AMs are associated with a SP-A-mediated direct modulation of the IkappaB-alpha turnover in these cells...
- Surfactant protein a inhibits lipopolysaccharide-induced immune cell activation by preventing the interaction of lipopolysaccharide with lipopolysaccharide-binding proteinCordula Stamme
Department of Immunochemistry and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Bioscience, Borstel, Germany
Am J Respir Cell Mol Biol 27:353-60. 2002..Therefore, the ability of SP-A to inhibit immune cell activation by Re-LPS may be due to its ability to block the binding of Re-LPS to LBP and prevent the initiation of the LBP/CD14 pathway for inflammatory reactions in the lung...
- Lipoteichoic acid (LTA) of Streptococcus pneumoniae and Staphylococcus aureus activates immune cells via Toll-like receptor (TLR)-2, lipopolysaccharide-binding protein (LBP), and CD14, whereas TLR-4 and MD-2 are not involvedNicolas W J Schröder
Institut für Mikrobiologie und Hygiene, Universitatsklinikum Charite, Medizinische Fakultat der Humboldt Universitat zu Berlin, Dorotheenstrasse 96, D 10117 Berlin, Germany
J Biol Chem 278:15587-94. 2003..However, the involvement of TLR-4 and MD-2 in this process was ruled out...
- CD55/decay accelerating factor is part of the lipopolysaccharide-induced receptor complexHolger Heine
Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany
Eur J Immunol 33:1399-408. 2003..Finally, through FRET analysis we could demonstrate LPS-induced clustering of CD55 and CD11/CD18 in human monocytes. Our results imply a new functional role of CD55 as a member of a multimeric LPS receptor complex...
- TLR4 polymorphisms, infectious diseases, and evolutionary pressure during migration of modern humansBart Ferwerda
Department of Internal Medicine, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
Proc Natl Acad Sci U S A 104:16645-50. 2007....
- Cutting edge: A common polymorphism impairs cell surface trafficking and functional responses of TLR1 but protects against leprosyChristopher M Johnson
Department of Microbiology, College of Medicine, University of Illinois, 601 South Goodwin Avenue, Urbana, IL 61801, USA
J Immunol 178:7520-4. 2007..Surprisingly, the 602S allele is associated with a decreased incidence of leprosy, suggesting that Mycobacterium leprae subverts the TLR system as a mechanism of immune evasion...
- Common polymorphisms of toll-like receptors 4 and 9 are associated with the clinical manifestation of malaria during pregnancyFrank P Mockenhaupt
Institute of Tropical Medicine and International Health, Berlin, Germany
J Infect Dis 194:184-8. 2006..These findings suggest that TLR4 and TLR9 play a role in the manifestation of malaria during pregnancy...
- Pre- and postoperative cytokine release after in vitro whole blood lipopolysaccharide stimulation and frequent toll-like receptor 4 polymorphismsOliver Kumpf
Robert Rossle Klinik, Klinik fur Chirurgie und Chirurgische Onkologie, Charite Universitatsmedizin Berlin, Campus Berlin Buch, Kindenberger Weg 80, 13125 Berlin, Germany
Shock 25:123-8. 2006..The LPS adaptation effect of cytokine release after surgery is furthermore not affected by the presence of the TLR4 polymorphism Asp299Gly/Thr399Ile...
- Toll-like receptor (TLR) polymorphisms in African children: Common TLR-4 variants predispose to severe malariaFrank P Mockenhaupt
Institute of Tropical Medicine Berlin, Charite Universitatsmedizin Berlin, Spandauer Damm 130, 14050 Berlin, Germany
Proc Natl Acad Sci U S A 103:177-82. 2006..5- and 2.6-fold increased risks of severe malaria, respectively. These findings suggest TLR4-mediated responses to malaria in vivo and TLR-4 polymorphisms to be associated with disease manifestation...
- High frequency of polymorphism Arg753Gln of the Toll-like receptor-2 gene detected by a novel allele-specific PCRNicolas W J Schröder
Institut für Mikrobiologie und Hygiene, Universitatsklinikum Charite, Medizinische Fakultat, Humboldt Universitat zu Berlin, Dorotheenstrasse 96, 10098, Berlin, Germany
J Mol Med (Berl) 81:368-72. 2003....
- A frequent toll-like receptor (TLR)-2 polymorphism is a risk factor for coronary restenosisLutz Hamann
Institute for Microbiology and Hygiene, Charite University Medical Center, Humboldt University, Dorotheenstrasse 96, 10117 Berlin, Germany
J Mol Med (Berl) 83:478-85. 2005..Screening for the TLR-2 Arg753Gln SNP may be of importance for stratifying a patient's risk and for preventive and therapeutic measures...
- Lipopolysaccharide binding protein binds to triacylated and diacylated lipopeptides and mediates innate immune responsesNicolas W J Schröder
Institut für Mikrobiologie und Hygiene, Charite University Medical Center, Humboldt University, Berlin, Germany
J Immunol 173:2683-91. 2004..These results support that LBP is a pattern recognition receptor transferring a variety of bacterial ligands including the two major types of lipopeptides to CD14 present in different receptor complexes...
- Use of locked nucleic acid oligonucleotides as hybridization/FRET probes for quantification of 16S rDNA by real-time PCROliver Goldenberg
Charite University Medical Center, Berlin, Germany
Biotechniques 38:29-30, 32. 2005