M Fleischhacker

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. ncbi Quantification of free RNA in serum and bronchial lavage: a new diagnostic tool in lung cancer detection?
    Bernd Schmidt
    Division of Pneumology, University Hospital Charite, Charite Universitatsmedizin Berlin, Campus Charite Mitte, Schumannstr 20 21, D 10117 Berlin, Germany
    Lung Cancer 48:145-7. 2005
  2. doi Free circulating nucleic acids in plasma and serum (CNAPS) -- Useful for the detection of lung cancer patients?
    Michael Fleischhacker
    Charite Universitatsmedizin Berlin, Med Klinik m S Onkologie und Hämatologie, Berlin, Germany
    Cancer Biomark 6:211-9. 2010
  3. pmc Correlation of SHOX2 gene amplification and DNA methylation in lung cancer tumors
    Katja U Schneider
    University Hospital Heidelberg, Institute of Human Genetics, Heidelberg, Germany
    BMC Cancer 11:102. 2011
  4. pmc SHOX2 DNA methylation is a biomarker for the diagnosis of lung cancer based on bronchial aspirates
    Bernd Schmidt
    Universitätsklinik und Poliklinik für Innere Medizin I, Universitätsklinikum Halle Saale, Halle, Germany
    BMC Cancer 10:600. 2010
  5. ncbi Biology of circulating mRNA: still more questions than answers?
    Michael Fleischhacker
    Charite Universitatsmedizin Berlin, Medizinische Klinik m S Onkologie u Hämatologie, Molekularbiologisches Labor, Alte Apotheke, Schumannstr 20 21, 10117 Berlin, Germany
    Ann N Y Acad Sci 1075:40-9. 2006
  6. ncbi 3rd International Symposium on Circulating Nucleic Acids in Plasma and Serum (CNAPS III). Santa Monica, California, November 9-12, 2003
    M Fleischhacker
    Universitatsmedizin Berlin, Medizinische Klinik II, Onkologie und Hämatologie, CCM, Berlin, Germany
    Eur J Med Res 9:87-93. 2004
  7. doi Methods for isolation of cell-free plasma DNA strongly affect DNA yield
    Michael Fleischhacker
    Charite, Universitatsmedizin Berlin, Med Klinik m S Onkologie u Hämatologie, Mol Biol Labor, Chariteplatz 1, 10117 Berlin, Germany
    Clin Chim Acta 412:2085-8. 2011
  8. ncbi Detection of amplifiable messenger RNA in the serum of patients with lung cancer
    M Fleischhacker
    Universitatsklinikum Charite, Medizinische Klinik II m S Hämatologie und Onkologie, Berlin, Germany
    Ann N Y Acad Sci 945:179-88. 2001
  9. ncbi The 2nd International Symposium on Circulating Nucleic Acids in Plasma and Serum (CNAPS-2), Hong Kong, February 20-21, 2001
    M Fleischhacker
    Universitatsklinikum Charite, Medizinische Klinik II m S Onkologie und Hämatologie, Schumannstr 20 21, D 10117 Berlin, Germany
    Eur J Med Res 6:364-8. 2001
  10. pmc Longitudinal genotyping of Candida dubliniensis isolates reveals strain maintenance, microevolution, and the emergence of itraconazole resistance
    M Fleischhacker
    Charitè Universitätsmedizin Berlin Medizinische Klinik m S Onkologie u Hämatologie, Mol Biol Labor, Alte Apotheke, CCM, Chariteplatz 1, 10117 Berlin, Germany
    J Clin Microbiol 48:1643-50. 2010

Collaborators

Detail Information

Publications30

  1. ncbi Quantification of free RNA in serum and bronchial lavage: a new diagnostic tool in lung cancer detection?
    Bernd Schmidt
    Division of Pneumology, University Hospital Charite, Charite Universitatsmedizin Berlin, Campus Charite Mitte, Schumannstr 20 21, D 10117 Berlin, Germany
    Lung Cancer 48:145-7. 2005
    ..009). In conclusion, quantification of intact RNA isolated from BLF supernatant and from serum might become a valuable tool for differentiation between tumor and non-tumor patients...
  2. doi Free circulating nucleic acids in plasma and serum (CNAPS) -- Useful for the detection of lung cancer patients?
    Michael Fleischhacker
    Charite Universitatsmedizin Berlin, Med Klinik m S Onkologie und Hämatologie, Berlin, Germany
    Cancer Biomark 6:211-9. 2010
    ..In addition, we highlight some technical points considering the pre-analytical probe handling and assay standardization, as well as the biology behind free-circulating nucleic acids...
  3. pmc Correlation of SHOX2 gene amplification and DNA methylation in lung cancer tumors
    Katja U Schneider
    University Hospital Heidelberg, Institute of Human Genetics, Heidelberg, Germany
    BMC Cancer 11:102. 2011
    ..An amplification of the SHOX2 gene locus together with the observed tumor-specific hypermethylation might explain the good performance of this marker in bronchial lavage samples...
  4. pmc SHOX2 DNA methylation is a biomarker for the diagnosis of lung cancer based on bronchial aspirates
    Bernd Schmidt
    Universitätsklinik und Poliklinik für Innere Medizin I, Universitätsklinikum Halle Saale, Halle, Germany
    BMC Cancer 10:600. 2010
    ..A test with a low false positive rate can reduce the need for further invasive and costly procedures and ensure early treatment...
  5. ncbi Biology of circulating mRNA: still more questions than answers?
    Michael Fleischhacker
    Charite Universitatsmedizin Berlin, Medizinische Klinik m S Onkologie u Hämatologie, Molekularbiologisches Labor, Alte Apotheke, Schumannstr 20 21, 10117 Berlin, Germany
    Ann N Y Acad Sci 1075:40-9. 2006
    ..In this short review I will discuss some basic aspects of the release mechanisms for the RNA, its biological meaning, and clinical value...
  6. ncbi 3rd International Symposium on Circulating Nucleic Acids in Plasma and Serum (CNAPS III). Santa Monica, California, November 9-12, 2003
    M Fleischhacker
    Universitatsmedizin Berlin, Medizinische Klinik II, Onkologie und Hämatologie, CCM, Berlin, Germany
    Eur J Med Res 9:87-93. 2004
    ..This meeting report summarizes the state of the art in the field...
  7. doi Methods for isolation of cell-free plasma DNA strongly affect DNA yield
    Michael Fleischhacker
    Charite, Universitatsmedizin Berlin, Med Klinik m S Onkologie u Hämatologie, Mol Biol Labor, Chariteplatz 1, 10117 Berlin, Germany
    Clin Chim Acta 412:2085-8. 2011
    ..0001). Comparable results were achieved by the use of the genes GAPDH and ERV while higher values were obtained by use of ß-globin. The laboratory site had only minor influence on DNA yield when manual extraction methods were used...
  8. ncbi Detection of amplifiable messenger RNA in the serum of patients with lung cancer
    M Fleischhacker
    Universitatsklinikum Charite, Medizinische Klinik II m S Hämatologie und Onkologie, Berlin, Germany
    Ann N Y Acad Sci 945:179-88. 2001
    ..The hnRNP-B1 mRNA was detectable in 14/18 sera, and Her2/neu-specific mRNA could be amplified from the serum of 7/18 patients. Combining the last two markers, we were able to detect all patients with a malignant lung tumor...
  9. ncbi The 2nd International Symposium on Circulating Nucleic Acids in Plasma and Serum (CNAPS-2), Hong Kong, February 20-21, 2001
    M Fleischhacker
    Universitatsklinikum Charite, Medizinische Klinik II m S Onkologie und Hämatologie, Schumannstr 20 21, D 10117 Berlin, Germany
    Eur J Med Res 6:364-8. 2001
    ....
  10. pmc Longitudinal genotyping of Candida dubliniensis isolates reveals strain maintenance, microevolution, and the emergence of itraconazole resistance
    M Fleischhacker
    Charitè Universitätsmedizin Berlin Medizinische Klinik m S Onkologie u Hämatologie, Mol Biol Labor, Alte Apotheke, CCM, Chariteplatz 1, 10117 Berlin, Germany
    J Clin Microbiol 48:1643-50. 2010
    ..In addition, isolates may acquire itraconazole resistance, even in the absence of prior azole therapy...
  11. ncbi Circulating nucleic acids (CNAs) and cancer--a survey
    M Fleischhacker
    Charite, Universitatsmedizin Berlin, Medizinische Klinik mS Onkologie u Hämatologie, CCM, Chariteplatz 1, 10117 Berlin, Germany
    Biochim Biophys Acta 1775:181-232. 2007
    ..At the RNA level the overexpression of tumor-associated genes was shown. These observations laid the foundation for the development of assays for an early detection of cancer as well as for other clinical means...
  12. ncbi Molecular genetic characteristics of lung cancer--useful as real' tumor markers?
    M Fleischhacker
    Medizinische Klinik und Poliklinik mit Schwerpunkt Hämatologie und Onkologie, Universitatsklinikum Charite, Berlin, Germany
    Lung Cancer 25:7-24. 1999
    ..Some of the molecular genetic features of lung tumor cells are summarized and the possibilities to use these characteristics as new tumor markers are discussed...
  13. ncbi Tumour markers - new aspects of an old discussion?
    M Fleischhacker
    Medizinische Klinik m S, Hämatologie und Onkologie, Charite, Campus Mitte, Schumannstr 20 21, D 10117 Berlin, Germany
    Eur J Med Res 4:144-8. 1999
    ..Therefore, the development of more sensitive and specific tumor markers is urgently needed. In this review, the possibilities of using molecular genetic characteristics of cancer as specific and sensitive tumor markers are discussed...
  14. ncbi Increased levels of vascular endothelial growth factor in bronchoalveolar lavage of patients with bronchial carcinoma effect of tumour activity and oxidative stress due to radio-chemotherapy?
    T Beinert
    Universitäts Klinikum Charité, Zentrum fur Innere Medizin, Medizinische Klinik und Poliklinik II, Schumannstrasse 20 21, D 10117 Berlin, Germany
    Eur J Med Res 4:328-34. 1999
    ..We tested the hypothesis that VEGF is additionally affected by oxidative stress due to anticancer therapy. Moreover, the suitability of epidermal growth factor (EGF) to estimate tumour activity was studied...
  15. ncbi Detection of tumor-specific mRNA in cell-free bronchial lavage supernatant in patients with lung cancer
    E Engel
    Medizinische Klinik II, Onkologie u Hämatologie, Charite Universitatsmedizin Berlin, Berlin, Germany
    Ann N Y Acad Sci 1022:140-6. 2004
    ..This may open new possibilities for the diagnosis of lung cancer...
  16. ncbi Oxidant-induced lung injury in anticancer therapy
    T Beinert
    Med Klinik m S Hämatologie und Onkologie, Charite, Campus Mitte, Schumannstr 20 21, D 10117 Berlin, Germany
    Eur J Med Res 4:43-53. 1999
    ..This review discusses mechanisms of ROS generation during radio-chemotherapy in the lung, antioxidant defense strategies and responses to oxidants, thereby assessing current diagnostic tools...
  17. ncbi Detection of cell-free nucleic acids in bronchial lavage fluid supernatants from patients with lung cancer
    B Schmidt
    Division of Pneumology, University Hospital Charite, Schumannstr 20 21, D 10117, Berlin, Germany
    Eur J Cancer 40:452-60. 2004
    ..Altogether, tumour-associated changes were detected in DNA samples from 47% of the patients and in RNA samples from all of the patients analysed...
  18. ncbi Further evidence for oxidant-induced vascular endothelial growth factor up-regulation in the bronchoalveolar lavage fluid of lung cancer patients undergoing radio-chemotherapy
    T Beinert
    Charite, Medizinische Klinik m S Onkologie und Hämatologie, Berlin, Germany
    J Cancer Res Clin Oncol 126:352-6. 2000
    ..In this study we aimed to reveal whether this increase was due to the therapy-associated intrapulmonary oxidative burden...
  19. ncbi Meeting report - First international symposium on circulating nucleic acids in plasma/serum - Implications in cancer diagnostics, prognosis or follow up and in prenatal diagnosis - Menthon France, August 18-20, 1999
    M Fleischhacker
    Universitatsklinikum Charite, Medizinische Klinik II, Onkologie und Hämatologie, Schumannstr 20 21, D 10117 Berlin, Germany Michael
    Eur J Med Res 4:488-90. 1999
    ....
  20. doi Diagnosis of chronic disseminated candidosis from liver biopsies by a novel PCR in patients with haematological malignancies
    M Fleischhacker
    Medizinische Klinik und Poliklinik II, Charité Campus Mitte der Humboldt Universität zu Berlin, Berlin, Germany
    Clin Microbiol Infect 18:1010-6. 2012
    ..In conclusion, the novel LCD chip technique examined in our study was able to detect fungal pathogens in liver biopsies from patients with haematological malignancies and suspected HCI/CDC but was negative in control biopsies...
  21. ncbi Paradoxical growth effects of the echinocandins caspofungin and micafungin, but not of anidulafungin, on clinical isolates of Candida albicans and C. dubliniensis
    M Fleischhacker
    Department of Internal Medicine, Div Oncology and Hematology, Charite Universitatsmedizin Berlin, Charite Campus Mitte, Chariteplatz 1, 10117 Berlin, Germany
    Eur J Clin Microbiol Infect Dis 27:127-31. 2008
    ..To analyze the effects of a high concentration of three antifungal substances, the echinocandins anidulafungin, caspofungin, and micafungin, on the growth of Candida spp...
  22. pmc Secretion of E,E-farnesol and biofilm formation in eight different Candida species
    K Weber
    Charite Universitatsmedizin Berlin, Department of Medicine, Division of Oncology and Hematology, Chariteplatz 1, 10117 Berlin, Germany
    Antimicrob Agents Chemother 52:1859-61. 2008
    ..FOH production differed significantly not only between Candida spp. but within Candida albicans strains as well. FOH concentrations and biofilm formation were the highest for C. albicans...
  23. ncbi Detection of cell-free DNA in bronchial lavage fluid supernatants of patients with lung cancer
    Tim Carstensen
    Charite Universitatsmedizin Berlin, Medizinische Klinik II, Pulmologie, Berlin, Germany
    Ann N Y Acad Sci 1022:202-10. 2004
    ..Their quantity and quality are sufficient for further amplification via polymerase chain reaction. Altogether, tumor-associated changes were detected in the DNA of 47% of the patients that were analyzed...
  24. doi Integrity of cell-free plasma DNA in patients with lung cancer and nonmalignant lung disease
    Bernd Schmidt
    Medizinische Klinik m S Infektiologie und Pneumologie, Pneumologie, Charite Universitatsmedizin, Berlin, Germany
    Ann N Y Acad Sci 1137:207-13. 2008
    ..Our results led us to conclude that this method is not useful in a diagnostic setting and is not able to differentiate between lung cancer patients and patients with a benign lung disease...
  25. doi An improved method for the isolation of free-circulating plasma DNA and cell-free DNA from other body fluids
    Christoph Kirsch
    MACHEREY NAGEL GmbH and Co KG, Duren, Germany
    Ann N Y Acad Sci 1137:135-9. 2008
    ..The procedure is fast and easy to standardize and might be valuable for laboratories working in the field of CNAPS...
  26. doi Cell-free DNA resuscitated for tumor testing
    Michael Fleischhacker
    Nat Med 14:914-5. 2008
  27. ncbi TP53 gene in blood plasma DNA of tumor patients
    Horst H Schlechte
    Department of Urology, Charité Hospital of the Humboldt University of Berlin, Berlin, Germany
    Ann N Y Acad Sci 1022:61-9. 2004
    ..Genetic analysis of plasma in tumor patients should be further developed, as it might be of prognostic value...
  28. ncbi Microsatellite alterations in free-circulating serum DNA in patients with ulcerative colitis
    Peter Rauh
    Charite Universitatsmedizin Berlin, Medizinische Klinik m S Onkologie und Haematologie, Campus Charite Mitte, Berlin, Deutschland
    Dig Dis 21:363-6. 2003
    ....
  29. ncbi Changes in concentration of DNA in serum and plasma during storage of blood samples
    Monika Jung
    Clin Chem 49:1028-9. 2003
  30. ncbi Improved method for isolating cell-free DNA
    Bernd Schmidt
    Clin Chem 51:1561-3. 2005