J V Holtje

Summary

Country: Germany

Publications

  1. pmc Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli
    J V Holtje
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Germany
    Microbiol Mol Biol Rev 62:181-203. 1998
  2. ncbi request reprint Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli
    W Vollmer
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Spemannstrasse 35, D 72076 Tubingen, Germany
    J Biol Chem 274:6726-34. 1999
  3. ncbi request reprint Cloning and characterization of PBP 1C, a third member of the multimodular class A penicillin-binding proteins of Escherichia coli
    G Schiffer
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, D 72076 Tubingen, Germany
    J Biol Chem 274:32031-9. 1999
  4. ncbi request reprint Specific interaction of penicillin-binding proteins 3 and 7/8 with soluble lytic transglycosylase in Escherichia coli
    T Romeis
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Federal Republic of Germany
    J Biol Chem 269:21603-7. 1994
  5. pmc Outer membrane localization of murein hydrolases: MltA, a third lipoprotein lytic transglycosylase in Escherichia coli
    J Lommatzsch
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    J Bacteriol 179:5465-70. 1997
  6. ncbi request reprint Alterations of murein structure and of penicillin-binding proteins in minicells from Escherichia coli
    W Obermann
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, FRG
    Microbiology 140:79-87. 1994
  7. pmc A simple screen for murein transglycosylase inhibitors
    W Vollmer
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, 72076 Tubingen, Germany
    Antimicrob Agents Chemother 44:1181-5. 2000
  8. pmc Interference with murein turnover has no effect on growth but reduces beta-lactamase induction in Escherichia coli
    A R Kraft
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, 72076 Tubingen, Germany
    J Bacteriol 181:7192-8. 1999
  9. ncbi request reprint Affinity chromatography as a means to study multienzyme complexes involved in murein synthesis
    M von Rechenberg
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    Microb Drug Resist 2:155-7. 1996
  10. ncbi request reprint A murein hydrolase is the specific target of bulgecin in Escherichia coli
    M F Templin
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Germany
    J Biol Chem 267:20039-43. 1992

Collaborators

Detail Information

Publications27

  1. pmc Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli
    J V Holtje
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Germany
    Microbiol Mol Biol Rev 62:181-203. 1998
    ..A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template...
  2. ncbi request reprint Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli
    W Vollmer
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Spemannstrasse 35, D 72076 Tubingen, Germany
    J Biol Chem 274:6726-34. 1999
    ..coli...
  3. ncbi request reprint Cloning and characterization of PBP 1C, a third member of the multimodular class A penicillin-binding proteins of Escherichia coli
    G Schiffer
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, D 72076 Tubingen, Germany
    J Biol Chem 274:32031-9. 1999
    ..Deletion of PBP1C resulted in an altered mode of murein synthesis. It is suggested that PBP1C functions in vivo as a transglycosylase only...
  4. ncbi request reprint Specific interaction of penicillin-binding proteins 3 and 7/8 with soluble lytic transglycosylase in Escherichia coli
    T Romeis
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Federal Republic of Germany
    J Biol Chem 269:21603-7. 1994
    ..In addition, PBP7/8 was found not only to stabilize but also to stimulate the enzymatic activity of Slt70 by a protein-protein interaction. It is concluded that Slt70, PBP7/8, and PBP3 may form a multienzyme complex in vivo...
  5. pmc Outer membrane localization of murein hydrolases: MltA, a third lipoprotein lytic transglycosylase in Escherichia coli
    J Lommatzsch
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    J Bacteriol 179:5465-70. 1997
    ..However, dramatic changes in the structure of the murein sacculus indicate that lytic transglycosylases are involved in maturation of the murein sacculus...
  6. ncbi request reprint Alterations of murein structure and of penicillin-binding proteins in minicells from Escherichia coli
    W Obermann
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, FRG
    Microbiology 140:79-87. 1994
    ..Whereas PBP4 and PBP6 were greatly diminished, PBP8 was significantly increased. We consider it unlikely that the sort of changes observed in murein structure reflect the fact that minicells are composed of two hemispherical polar caps...
  7. pmc A simple screen for murein transglycosylase inhibitors
    W Vollmer
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, 72076 Tubingen, Germany
    Antimicrob Agents Chemother 44:1181-5. 2000
    ..Crude membrane extracts can be used as a source for the PBPs, and different kinds of labels for the penicillin-PBP complexes can be used. The assay can be adapted to high-throughput screens...
  8. pmc Interference with murein turnover has no effect on growth but reduces beta-lactamase induction in Escherichia coli
    A R Kraft
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, 72076 Tubingen, Germany
    J Bacteriol 181:7192-8. 1999
    ..Thus, specific inhibitors of lytic transglycosylases can increase the potency of penicillins and cephalosporins against bacteria inducing AmpC-like beta-lactamases...
  9. ncbi request reprint Affinity chromatography as a means to study multienzyme complexes involved in murein synthesis
    M von Rechenberg
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    Microb Drug Resist 2:155-7. 1996
    ..It is speculated that the lytic transglycosylases assemble a multienzyme complex consisting of hydrolases and synthases, which is involved in growth of the stress-bearing murein sacculus...
  10. ncbi request reprint A murein hydrolase is the specific target of bulgecin in Escherichia coli
    M F Templin
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Germany
    J Biol Chem 267:20039-43. 1992
    ..The possibility of an allosteric modulation of the activity of the enzyme by changes in the structure of the murein sacculus is discussed...
  11. pmc Membrane-bound lytic endotransglycosylase in Escherichia coli
    A R Kraft
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    J Bacteriol 180:3441-7. 1998
    ....
  12. ncbi request reprint Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli
    C Heidrich
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Spemannstrasse 35, 72076 Tubingen, Germany
    Mol Microbiol 41:167-78. 2001
    ..As amidase deletion mutants accumulate trimeric and tetrameric cross-links in their murein, it is suggested that these structures mark the division site before cleavage of the septum...
  13. ncbi request reprint Characterization of three different lytic transglycosylases in Escherichia coli
    T Romeis
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, FRG
    FEMS Microbiol Lett 111:141-6. 1993
    ..The antibiotic bulgecin that inhibits Slt70 does not inhibit the lytic transglycosylases present in the slt70 deletion mutant...
  14. pmc Purification and properties of a membrane-bound lytic transglycosylase from Escherichia coli
    A Ursinus
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    J Bacteriol 176:338-43. 1994
    ..The amino acid sequences of four proteolytic peptides showed no identity with known sequences in the data bank. With Mlt38, the number of proteins in E. coli showing lytic transglycosylase activity rises to three...
  15. ncbi request reprint Enzymology of elongation and constriction of the murein sacculus of Escherichia coli
    J V Holtje
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Spemannstrasse 35, 72076, Tubingen, Germany
    Biochimie 83:103-8. 2001
    ..Such enzymes would at the same time cleave bonds in the murein net and insert new material into the growing sacculus...
  16. ncbi request reprint Control of the activity of the soluble lytic transglycosylase by the stringent response in Escherichia coli
    A S Betzner
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, F R G
    FEMS Microbiol Lett 55:161-4. 1990
    ..These findings indicate that the stringent control modulates the enzymatic activity of the soluble lytic transglycosylase in vivo...
  17. pmc A defect in cell wall recycling triggers autolysis during the stationary growth phase of Escherichia coli
    M F Templin
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, Spemannstrasse 35, 72076 Tubingen, Germany
    EMBO J 18:4108-17. 1999
    ..They can only function as acceptors and not as donors in the crucial cross-linking reaction. It is concluded that murein recycling is a promising target for novel antibacterial agents...
  18. ncbi request reprint Morphogenesis of Escherichia coli
    W Vollmer
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Spemannstrasse 35, D 72076, Tubingen, Germany
    Curr Opin Microbiol 4:625-33. 2001
    ..In this review, we summarize the recent results on murein structure and metabolism. On the basis of these findings, we present a model that explains maintenance of the specific rod shape of E. coli...
  19. pmc Subcellular distribution of the soluble lytic transglycosylase in Escherichia coli
    B Walderich
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    J Bacteriol 173:5668-76. 1991
    ..This finding is discussed in the light of recent evidence suggesting that the murein of E. coli might be a structure of more than one layer expanding by inside-to-outside growth of patches of murein...
  20. ncbi request reprint Lysis induction of Escherichia coli by the cloned lysis protein of the phage MS2 depends on the presence of osmoregulatory membrane-derived oligosaccharides
    J V Holtje
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Federal Republic of Germany
    J Biol Chem 263:3539-41. 1988
    ..It is concluded that the MDOs play an important role in maintaining a proper arrangement of inner and outer membrane, a prerequisite for a functional insertion of the MS2 lysis protein...
  21. ncbi request reprint Molecular interplay of murein synthases and murein hydrolases in Escherichia coli
    J V Holtje
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Germany
    Microb Drug Resist 2:99-103. 1996
    ....
  22. ncbi request reprint From growth to autolysis: the murein hydrolases in Escherichia coli
    J V Holtje
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Germany
    Arch Microbiol 164:243-54. 1995
    ..The murein turnover products that are released during growth are further degraded by these (1 --> 6)-anhydromuramic acid derivatives by an intramolecular transglycosylation reaction...
  23. ncbi request reprint Determination of murein precursors during the cell cycle of Escherichia coli
    U Kohlrausch
    Max Planck Institut fur Entwicklungsbiologie, Abteilung, Biochemie, Tubingen, FRG
    J Gen Microbiol 135:1499-506. 1989
    ..It is concluded that elongation and division of the murein sacculus is not controlled by oscillations in the concentrations of these late murein precursors...
  24. pmc Analysis of murein and murein precursors during antibiotic-induced lysis of Escherichia coli
    U Kohlrausch
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, Federal Republic of Germany
    J Bacteriol 173:3425-31. 1991
    ..coli. It is proposed that antibiotic-induced bacteriolysis is the result of a zipperlike splitting of the murein net by exo-muramidases locally restricted to the equatorial zone of the cell...
  25. ncbi request reprint The negative regulator of beta-lactamase induction AmpD is a N-acetyl-anhydromuramyl-L-alanine amidase
    J V Holtje
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, FRG
    FEMS Microbiol Lett 122:159-64. 1994
    ..It is suggested that the negative regulatory effect of AmpD is due to the hydrolysis of anhydro-muropeptides which may function as signals for beta-lactamase induction...
  26. ncbi request reprint Penicillin-binding protein 7/8 of Escherichia coli is a DD-endopeptidase
    T Romeis
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Germany
    Eur J Biochem 224:597-604. 1994
    ..The enzyme is inhibited by the 'penem' beta-lactam antibiotic CGP31608 at a concentration of 0.25 micrograms/ml by 50%. Thus besides PBP4 and the mepA gene product, a third endopeptidase exists in E. coli...
  27. ncbi request reprint Cloning and expression of a murein hydrolase lipoprotein from Escherichia coli
    K Ehlert
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Germany
    Mol Microbiol 16:761-8. 1995
    ..It is concluded that the previously isolated Slt35 protein is a proteolytic degradation product of the murein hydrolase lipoprotein MltB. Surprisingly, a deletion in the mltB gene showed no obvious phenotype...