Gerhard Heusipp

..These data suggest that under Ca(2+)-depleted conditions Tir might be secreted into the medium and integrated into host membranes by an Esp-independent mechanism, without the need for a functional type III translocation machinery...

..coli, led to the induction of htrA expression via the Cpx system in Y. enterocolitica. Interestingly, a Y. enterocolitica CpxR mutant strain was not impaired for virulence in a mouse model of infection...

..CCR6-deficient mice may therefore represent a suitable model for the study of M-cell function in vivo...

..Here, we summarize the current knowledge about the influence of DNA methylation on virulence functions and discuss perspectives for future research...

..coli, sigmaE of Y. enterocolitica is not induced by heat shock or ethanol. Overproduction of the outer membrane protein Ail does not lead to the induction of rpoE expression. However, rpoE expression is induced by osmotic stress...

..The data suggest that AAT has additional functions in the host response against bacterial infections that are not related to its anti-protease activity...

..coli genome. Moreover, this implies that the important identification of specific pathotypes has to be based on a broad matrix of indicator genes. In addition, the presence of intermediate strains needs to be accounted for...

..This is the first example of posttranslational regulation of type III secretion by the Clp protease and adds a new flavor to the complex regulatory mechanisms underlying the controlled release of effector proteins from bacterial cells...

..Our data describe the transcriptional regulation of the tad type IVb pilus operon by PypB in Y. enterocolitica but fail to show most previously described phenotypes associated with this type of pilus in other bacteria...

..enterocolitica and indicate that Dam targets regulatory processes modulating the composition and function of the bacterial surface...

..As ChiY and EngY both bind to chitin, and YE3650 shows motifs conserved in oligosaccharide-binding enzymes, all secreted proteins might be important for polysaccharide interaction/degradation during infection or in the environment...

..In addition, we show that cpxR and cpxP mutant strains have no defect in Y. enterocolitica invasion of host cells...

..Furthermore, Dam mediates virulence of Y. enterocolitica. Dam overproduction results in increased tissue culture invasion of Y. enterocolitica, while the expression of specifically in vivo-expressed genes is not altered...

..Furthermore, it can lead to important advances in understanding and evaluating the intracellular and molecular function of YopM without the need for infection with Yersinia...

..Our data reveal a new regulatory network that might have implications for the controlled expression of further virulence-associated functions in Yersinia...

..We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2...

..Homologues of the novel effector Ibe are widely distributed among EPEC, ATEC and EHEC strains but are not necessarily genetically linked to the LEE as they have occasionally also been found to be encoded within lambdoid prophages...

..In this study, we identify AAT as a new binding partner for EspB and EspD, suggesting a previously unappreciated role for AAT in host cell defense against EPEC infections and potentially also against other bacterial pathogens...

..While the H-NS antagonist RovA activates pypC, it has no effect on pypA and pypB. Furthermore, H-NS affects biofilm formation in Y. enterocolitica...

..We suggest that these findings reflect early steps in the development of neurological disorders associated with pertussis disease...

..Together, our results demonstrate that under conditions of limited Ca(2+) an alternative mechanism for Tir integration can trigger the induction of A/E lesions...