Ursula Bilitewski

Summary

Affiliation: Helmholtz Centre for Infection Research
Country: Germany

Publications

  1. pmc Deletion of the HAMP domains from the histidine kinase CaNik1p of Candida albicans or treatment with fungicides activates the MAP kinase Hog1p in S. cerevisiae transformants
    Mohammed El-Mowafy
    AG Biological Systems Analysis, Helmholtz Centre for Infection Research HZI, Inhoffenstr, 7, 38124 Braunschweig, Germany
    BMC Microbiol 13:209. 2013
  2. ncbi request reprint Determination of immunomodulatory effects: focus on functional analysis of phagocytes as representatives of the innate immune system
    Ursula Bilitewski
    Helmholtz Centre for Infection Research HZI, Inhoffenstr 7, 38124, Braunschweig, Germany
    Anal Bioanal Chem 391:1545-54. 2008
  3. doi request reprint A novel functional assay for fungal histidine kinases group III reveals the role of HAMP domains for fungicide sensitivity
    Anna Buschart
    Helmholtz Centre for Infection Research, Department of Biological Systems Analysis, Inhoffenstr 7, 38124 Braunschweig, Germany
    J Biotechnol 157:268-77. 2012
  4. doi request reprint The fungicide fludioxonil antagonizes fluconazole activity in the human fungal pathogen Candida albicans
    Anna Buschart
    Biological Systems Analysis, Helmholtz Centre for Infection Research, Braunschweig, Germany
    J Med Microbiol 61:1696-703. 2012
  5. doi request reprint A viability assay for Candida albicans based on the electron transfer mediator 2,6-dichlorophenolindophenol
    Rabeay Y A Hassan
    Biological Systems Analysis Group, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany
    Anal Biochem 419:26-32. 2011
  6. doi request reprint Deletion of the Candida albicans histidine kinase gene CHK1 improves recognition by phagocytes through an increased exposure of cell wall beta-1,3-glucans
    Nina Klippel
    Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany
    Microbiology 156:3432-44. 2010
  7. ncbi request reprint Development of an enzyme-linked immunoreceptor assay (ELIRA) for quantification of the biological activity of recombinant human bone morphogenetic protein-2
    Janine Wendler
    Department of Natural Product Biology, National Research Centre for Biotechnology GBF, Mascheroder Weg 1, 38124 Braunschweig, Germany
    J Biotechnol 119:425-35. 2005
  8. doi request reprint Direct electrochemical determination of Candida albicans activity
    Rabeay Y A Hassan
    Helmholtz Centre for Infection Research, Working Group BiSA, Inhoffenstr 7, 38124 Braunschweig, Germany
    Biosens Bioelectron 49:192-8. 2013
  9. doi request reprint Identification of inhibitors of yeast-to-hyphae transition in Candida albicans by a reporter screening assay
    Anna Heintz-Buschart
    Department of Biological Systems Analysis, Helmholtz Centre for Infection Research, Inhoffenstr 7, 38124 Braunschweig, Germany
    J Biotechnol 164:137-42. 2013
  10. pmc Involvement of the mitogen activated protein kinase Hog1p in the response of Candida albicans to iron availability
    Hani E J Kaba
    Working Group Biological Systems Analysis, Helmholtz Centre for Infection Research, Inhoffenstr 7, D 38124 Braunschweig, Germany
    BMC Microbiol 13:16. 2013

Collaborators

  • Janine Wendler
  • Mahmoud M Bahgat
  • Ursula Rinas
  • Andrea Hoffmann
  • Rabeay Y A Hassan
  • Anna Buschart
  • Shuna Cui
  • Mohammed El-Mowafy
  • Nina Klippel
  • Hani E J Kaba
  • Anna Heintz-Buschart
  • Janine Wesolowski
  • Judith Behnsen
  • Nina Wienhoefer
  • Peter P Müller
  • Erwin Hohn
  • Manfred Nimtz
  • Holger Eickhoff
  • Peter P Mueller
  • Joop van den Heuvel
  • Katja Gremmer
  • Anna Burakowska
  • Lothar Groebe
  • Stephan Hodde
  • Christian Bardroff
  • Matthias Gunzer
  • Matthias Brock
  • Frank Gunzer
  • Mike Hasenberg
  • Priyanka Narang
  • Manfred Rohde
  • Axel A Brakhage

Detail Information

Publications15

  1. pmc Deletion of the HAMP domains from the histidine kinase CaNik1p of Candida albicans or treatment with fungicides activates the MAP kinase Hog1p in S. cerevisiae transformants
    Mohammed El-Mowafy
    AG Biological Systems Analysis, Helmholtz Centre for Infection Research HZI, Inhoffenstr, 7, 38124 Braunschweig, Germany
    BMC Microbiol 13:209. 2013
    ..Heterologous expression of this protein in Sacchromyces cerevisiae conferred susceptibility to different fungicides. Fungicide activity was associated with phosphorylation of the mitogen activated protein kinase Hog1p...
  2. ncbi request reprint Determination of immunomodulatory effects: focus on functional analysis of phagocytes as representatives of the innate immune system
    Ursula Bilitewski
    Helmholtz Centre for Infection Research HZI, Inhoffenstr 7, 38124, Braunschweig, Germany
    Anal Bioanal Chem 391:1545-54. 2008
    ..Corresponding analytical procedures are available, but standardization is required, as varying the procedure may influence the outcomes of the assays...
  3. doi request reprint A novel functional assay for fungal histidine kinases group III reveals the role of HAMP domains for fungicide sensitivity
    Anna Buschart
    Helmholtz Centre for Infection Research, Department of Biological Systems Analysis, Inhoffenstr 7, 38124 Braunschweig, Germany
    J Biotechnol 157:268-77. 2012
    ..This indicates that the HAMP domains are decisive for the mode of action of the antifungal compounds...
  4. doi request reprint The fungicide fludioxonil antagonizes fluconazole activity in the human fungal pathogen Candida albicans
    Anna Buschart
    Biological Systems Analysis, Helmholtz Centre for Infection Research, Braunschweig, Germany
    J Med Microbiol 61:1696-703. 2012
    ..The resistance to fluconazole was increased by fludioxonil, independently of Nik1p. Therefore, exposure of C. albicans to fludioxonil may lead to increased resistance to fluconazole treatment...
  5. doi request reprint A viability assay for Candida albicans based on the electron transfer mediator 2,6-dichlorophenolindophenol
    Rabeay Y A Hassan
    Biological Systems Analysis Group, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany
    Anal Biochem 419:26-32. 2011
    ..albicans but could be increased to the C. albicans level by adding glucose. No significant increases were observed with galactose. DCIP reduction rates from C. albicans were not further increased by any carbon source...
  6. doi request reprint Deletion of the Candida albicans histidine kinase gene CHK1 improves recognition by phagocytes through an increased exposure of cell wall beta-1,3-glucans
    Nina Klippel
    Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany
    Microbiology 156:3432-44. 2010
    ..As there are no homologues of fungal histidine kinases in mammals, Chk1p has to be considered as a promising target for new antifungal agents...
  7. ncbi request reprint Development of an enzyme-linked immunoreceptor assay (ELIRA) for quantification of the biological activity of recombinant human bone morphogenetic protein-2
    Janine Wendler
    Department of Natural Product Biology, National Research Centre for Biotechnology GBF, Mascheroder Weg 1, 38124 Braunschweig, Germany
    J Biotechnol 119:425-35. 2005
    ....
  8. doi request reprint Direct electrochemical determination of Candida albicans activity
    Rabeay Y A Hassan
    Helmholtz Centre for Infection Research, Working Group BiSA, Inhoffenstr 7, 38124 Braunschweig, Germany
    Biosens Bioelectron 49:192-8. 2013
    ..We believe that this work can be utilized for the development of early detection assays and opens the door for new technological developments in the field of C. albicans...
  9. doi request reprint Identification of inhibitors of yeast-to-hyphae transition in Candida albicans by a reporter screening assay
    Anna Heintz-Buschart
    Department of Biological Systems Analysis, Helmholtz Centre for Infection Research, Inhoffenstr 7, 38124 Braunschweig, Germany
    J Biotechnol 164:137-42. 2013
    ..In conclusion we developed a novel screening assay, which addresses the morphological switch from the yeast form of C. albicans to its hyphal form and identified novel chemical structures with activity against C. albicans...
  10. pmc Involvement of the mitogen activated protein kinase Hog1p in the response of Candida albicans to iron availability
    Hani E J Kaba
    Working Group Biological Systems Analysis, Helmholtz Centre for Infection Research, Inhoffenstr 7, D 38124 Braunschweig, Germany
    BMC Microbiol 13:16. 2013
    ..albicans to iron availability was not studied in detail. Thus, we analyzed phenotypic and molecular responses of C. albicans to different iron concentrations particularly with respect to the activity of the Hog1p MAP kinase module...
  11. ncbi request reprint Sensing of oxygen in microtiter plates: a novel tool for screening drugs against pathogenic yeasts
    Janine Wesolowski
    Helmholtz Centre for Infection Research HZI, Inhoffenstr 7, 38124, Braunschweig, Germany
    Anal Bioanal Chem 391:1731-7. 2008
    ..Thus, the determination of oxygen in microtiter plates via fluorescent dyes is a versatile supplement to standard growth inhibition tests...
  12. ncbi request reprint Application of an SPR-based receptor assay for the determination of biologically active recombinant bone morphogenetic protein-2
    Janine Wendler
    Division of Natural Products, National Research Centre for Biotechnology GBF, Mascheroder Weg 1, 38124, Braunschweig, Germany
    Anal Bioanal Chem 381:1056-64. 2005
    ..These data show that the disulphide-bonded dimer corresponds to the biologically active protein capable of binding the BMP-receptor type IA...
  13. doi request reprint Genistein induces morphology change and G2/M cell cycle arrest by inducing p38 MAPK activation in macrophages
    Shuna Cui
    Medical College of Yangzhou University, Huaihai Road 11, 225001, Yangzhou, China Helmholtz Centre for Infection Research HZI, Inhoffenstr 7, 38124 Braunschweig, Germany
    Int Immunopharmacol 18:142-50. 2014
    ..The production of cytokines (TNF-α) stimulated by C. albicans was strongly inhibited by genistein. In conclusion, genistein showed a strong immune modulatory effect on the macrophages. ..
  14. ncbi request reprint Biochemical analysis with microfluidic systems
    Ursula Bilitewski
    Dept Natural Products Biology, Gesellschaft fur Biotechnologische Forschung mbH, Mascheroder Weg 1, 38124, Braunschweig, Germany
    Anal Bioanal Chem 377:556-69. 2003
    ..They utilize either affinity capillary electrophoresis as a homogeneous assay format, or immobilized antigens or antibodies in heterogeneous assays with serial supply of reagents and washing solutions...
  15. pmc Environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans
    Judith Behnsen
    Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Jena, Germany
    PLoS Pathog 3:e13. 2007
    ..This could explain why "delocalized" Aspergillus infections such as hematogeneous spread are almost uncontrollable diseases, even in immunocompetent individuals...