Stefan W Hell

Summary

Country: Germany

Publications

  1. ncbi request reprint Breaking the diffraction barrier in fluorescence microscopy by optical shelving
    Stefan Bretschneider
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Phys Rev Lett 98:218103. 2007
  2. doi request reprint Dual-color STED microscopy at 30-nm focal-plane resolution
    Lars Meyer
    Max Planck Institute for Biophysical Chemistry, Dept of NanoBiophotonics, 37070 Gottingen, Germany
    Small 4:1095-100. 2008
  3. ncbi request reprint Toward fluorescence nanoscopy
    Stefan W Hell
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 21:1347-55. 2003
  4. ncbi request reprint Nanoscale resolution in GFP-based microscopy
    Katrin I Willig
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 3:721-3. 2006
  5. doi request reprint Spherical nanosized focal spot unravels the interior of cells
    Roman Schmidt
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 5:539-44. 2008
  6. doi request reprint Microscopy and its focal switch
    Stefan W Hell
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37070 Gottingen, Germany
    Nat Methods 6:24-32. 2009
  7. ncbi request reprint Concepts for nanoscale resolution in fluorescence microscopy
    Stefan W Hell
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37070 Gottingen, Germany
    Curr Opin Neurobiol 14:599-609. 2004
  8. doi request reprint Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 26:1035-40. 2008
  9. doi request reprint Comparing video-rate STED nanoscopy and confocal microscopy of living neurons
    Marcel A Lauterbach
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Biophotonics 3:417-24. 2010
  10. doi request reprint New fluorinated rhodamines for optical microscopy and nanoscopy
    Gyuzel Yu Mitronova
    Department of Nano Biophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen Germany, Fax 49 551 2012505
    Chemistry 16:4477-88. 2010

Collaborators

Detail Information

Publications95

  1. ncbi request reprint Breaking the diffraction barrier in fluorescence microscopy by optical shelving
    Stefan Bretschneider
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Phys Rev Lett 98:218103. 2007
    ..The presence of dark states in virtually any fluorescent molecule opens up a new venue for far-field microscopy with resolution that is no longer limited by diffraction...
  2. doi request reprint Dual-color STED microscopy at 30-nm focal-plane resolution
    Lars Meyer
    Max Planck Institute for Biophysical Chemistry, Dept of NanoBiophotonics, 37070 Gottingen, Germany
    Small 4:1095-100. 2008
  3. ncbi request reprint Toward fluorescence nanoscopy
    Stefan W Hell
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 21:1347-55. 2003
    ..Relying on saturated optical transitions, these concepts are limited only by the attainable saturation level. As strong saturation should be feasible at low light intensities, nanoscale imaging with focused light may be closer than ever...
  4. ncbi request reprint Nanoscale resolution in GFP-based microscopy
    Katrin I Willig
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 3:721-3. 2006
    ..Our results mark the advent of nanoscale biological microscopy with genetically encoded markers...
  5. doi request reprint Spherical nanosized focal spot unravels the interior of cells
    Roman Schmidt
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 5:539-44. 2008
    ..Fully relying on focused light, this lens-based fluorescence nanoscope unravels the interior of cells noninvasively, uniquely dissecting their sub-lambda-sized organelles...
  6. doi request reprint Microscopy and its focal switch
    Stefan W Hell
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37070 Gottingen, Germany
    Nat Methods 6:24-32. 2009
    ..Here I discuss the principles of these methods together with their differences in implementation and operation. Finally, I outline potential developments...
  7. ncbi request reprint Concepts for nanoscale resolution in fluorescence microscopy
    Stefan W Hell
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37070 Gottingen, Germany
    Curr Opin Neurobiol 14:599-609. 2004
    ..Formed on the basis of reversible saturable optical transitions, these concepts might eventually allow us to investigate hitherto inaccessible details within live cells...
  8. doi request reprint Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 26:1035-40. 2008
    ..Furthermore, we demonstrate dual-color fluorescence microscopy with sub-diffraction resolution using bsDronpa and Dronpa whose emission maxima are separated by <20 nm...
  9. doi request reprint Comparing video-rate STED nanoscopy and confocal microscopy of living neurons
    Marcel A Lauterbach
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Biophotonics 3:417-24. 2010
    ..Tentatively providing a larger photon flux, CW beams should facilitate extending fast STED imaging towards imaging fainter living samples...
  10. doi request reprint New fluorinated rhodamines for optical microscopy and nanoscopy
    Gyuzel Yu Mitronova
    Department of Nano Biophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen Germany, Fax 49 551 2012505
    Chemistry 16:4477-88. 2010
    ..We exemplify the excellent performance of the fluorinated rhodamines in optical microscopy by fluorescence correlation spectroscopy (FCS) and stimulated emission depletion (STED) nanoscopy experiments. ..
  11. doi request reprint A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching
    Tanja Brakemann
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
    Nat Biotechnol 29:942-7. 2011
    ..The switching properties of Dreiklang enable far-field fluorescence nanoscopy in living mammalian cells using both a coordinate-targeted and a stochastic single molecule switching approach...
  12. pmc Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron
    Tanja Brakemann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Biol Chem 285:14603-9. 2010
    ..Distinct absorption cross-sections for the switching wavelengths in the fluorescent and the nonfluorescent state are not essential for efficient photochromism in fluorescent proteins, although they may facilitate the switching process...
  13. pmc Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy
    Andre C Stiel
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 95:2989-97. 2008
    ..We demonstrate time-lapse live-cell subdiffraction microscopy by imaging rsCherryRev targeted to the endoplasmic reticulum utilizing the switching and localization of single molecules...
  14. pmc 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants
    Andre C Stiel
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Biochem J 402:35-42. 2007
    ..The findings reported in the present study support the view that a cis-trans isomerization is one of the key events common to the switching mechanism in RSFPs...
  15. doi request reprint Diffraction-unlimited all-optical imaging and writing with a photochromic GFP
    Tim Grotjohann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 478:204-8. 2011
    ..The reversible switching also enables all-optical writing of features with subdiffraction size and spacings, which can be used for data storage...
  16. pmc Endosomal sorting of readily releasable synaptic vesicles
    Peer Hoopmann
    European Neuroscience Institute, Deutsche Forschungsgemeinschaft Research Center for Molecular Physiology of the Brain Excellence Cluster 171, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 107:19055-60. 2010
    ..In the latter compartment, they segregated from plasma membrane components in a process that is likely important for sorting/budding of newly developed vesicles from the endosome...
  17. doi request reprint Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers
    Jonas Fölling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemphyschem 9:321-6. 2008
    ..Future synthesis of similar compounds holds great promise for cost-effective fluorescence nanoscopy with noninvasive optical sectioning...
  18. doi request reprint Nanoscopy of living brain slices with low light levels
    Ilaria Testa
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Neuron 75:992-1000. 2012
    ....
  19. pmc Structural basis for reversible photoswitching in Dronpa
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 104:13005-9. 2007
    ..We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore...
  20. ncbi request reprint Nanoscale separation of molecular species based on their rotational mobility
    Ilaria Testa
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 16:21093-104. 2008
    ..Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells...
  21. ncbi request reprint Direct observation of the nanoscale dynamics of membrane lipids in a living cell
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 457:1159-62. 2009
    ..The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells...
  22. pmc Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength
    Ilaria Testa
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 99:2686-94. 2010
    ..The method can be expanded to even more colors by choosing optimized dichroic mirrors and selecting marker molecules with negligible inhomogeneous emission broadening...
  23. doi request reprint Dual-label STED nanoscopy of living cells using photochromism
    Katrin I Willig
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
    Nano Lett 11:3970-3. 2011
    ..The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells...
  24. ncbi request reprint Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
    Mariano Bossi
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Nano Lett 8:2463-8. 2008
    ..The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques...
  25. ncbi request reprint STED microscopy with continuous wave beams
    Katrin I Willig
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 4:915-8. 2007
    ..Viable for fluorophores with low triplet yield, the use of CW light sources greatly simplifies the implementation of this concept of far-field fluorescence nanoscopy...
  26. ncbi request reprint Anatomy and dynamics of a supramolecular membrane protein cluster
    Jochen J Sieber
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Science 317:1072-6. 2007
    ....
  27. pmc Phosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment
    Alf Honigmann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Nat Struct Mol Biol 20:679-86. 2013
    ..Our results suggest that PIP2 clusters organized by syntaxin-1 act as molecular beacons for vesicle docking, with the subsequent Ca(2+) influx bringing the vesicle membrane close enough for membrane fusion...
  28. doi request reprint MRT letter: Nanoscopy of protein colocalization in living cells by STED and GSDIM
    Birka Lalkens
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Gottingen, Germany
    Microsc Res Tech 75:1-6. 2012
    ..Images with resolution down to 65 nm prove a powerful new way for studying protein colocalization in living cells at the nanoscale...
  29. doi request reprint Enhancing fluorescence brightness: effect of reverse intersystem crossing studied by fluorescence fluctuation spectroscopy
    Christian Ringemann
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Chemphyschem 9:612-24. 2008
    ..The study of ReISC not only results in a better understanding of a fluorescent label's photophysics, but the method is a possible approach to optimize fluorescence emission in experiments, where signal strength is a critical parameter...
  30. doi request reprint Video-rate far-field optical nanoscopy dissects synaptic vesicle movement
    Volker Westphal
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen 37077, Germany
    Science 320:246-9. 2008
    ..Our study demonstrates the emerging ability of optical microscopy to investigate intracellular physiological processes on the nanoscale in real time...
  31. doi request reprint 3D reconstruction of high-resolution STED microscope images
    Annedore Punge
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Microsc Res Tech 71:644-50. 2008
    ..This approach allows a 3D image reconstruction with a resolution <80 nm in all directions using available state-of-the art STED microscopes...
  32. ncbi request reprint Resolution scaling in STED microscopy
    Benjamin Harke
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 16:4154-62. 2008
    ..For relevant saturation values, the generalized square-root law is shown to predict the practical resolution with high accuracy...
  33. doi request reprint Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores
    Daniel Aquino
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
    Nat Methods 8:353-9. 2011
    ..Offering a combination of multicolor recording, nanoscale resolution and extended axial depth, our method substantially advances the noninvasive 3D imaging of cells and of other transparent materials...
  34. doi request reprint Fluorescence nanoscopy by ground-state depletion and single-molecule return
    Jonas Fölling
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 5:943-5. 2008
    ..Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach...
  35. ncbi request reprint Isotropic 3D Nanoscopy based on single emitter switching
    Claas von Middendorff
    Max Planck Institute, Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 16:20774-88. 2008
    ..We verify the results by Monte-Carlo simulations of the imaging process and by applying a simple maximum-likelihood estimator for position determination...
  36. doi request reprint Fast STED microscopy with continuous wave fiber lasers
    Gael Moneron
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Opt Express 18:1302-9. 2010
    ..2 s...
  37. pmc Stimulated emission depletion nanoscopy of living cells using SNAP-tag fusion proteins
    Birka Hein
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
    Biophys J 98:158-63. 2010
    ..Hence fusion proteins that bind modified organic dyes expand widely the application range of far-field fluorescence nanoscopy of living cells...
  38. pmc Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters
    Alexander Egner
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 93:3285-90. 2007
    ..These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm...
  39. ncbi request reprint Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching
    Miriam A Schwentker
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Microsc Res Tech 70:269-80. 2007
    ..The obtained resolution of 50 nm ( approximately lambda/12) is limited only by the spectroscopic properties of the proteins and the imperfections of the optical implementation, but not on principle grounds...
  40. doi request reprint Far-field optical nanoscopy with reduced number of state transition cycles
    Thorsten Staudt
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 19:5644-57. 2011
    ..Thus, the photobleaching of the sample is reduced, while resolution and recording speed are preserved. The power of the method is exemplified by imaging immunolabeled glial cells with up to 8-fold reduced photobleaching...
  41. ncbi request reprint Two-photon excitation STED microscopy
    Gael Moneron
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 17:14567-73. 2009
    ..4-fold improvement over the diffraction barrier...
  42. doi request reprint Mitochondrial cristae revealed with focused light
    Roman Schmidt
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37077 Gottingen, Germany
    Nano Lett 9:2508-10. 2009
    ..We find a pronounced heterogeneity in the cristae arrangements even within individual mitochondrial tubules...
  43. ncbi request reprint Limited intermixing of synaptic vesicle components upon vesicle recycling
    Felipe Opazo
    European Neuroscience Institute Göttingen, DFG Research Center for Molecular Physiology of the Brain Excellence Cluster 171, Grisebachstrs 5, 37077 Gottingen, Germany
    Traffic 11:800-12. 2010
    ..We suggest that several pHluorin-tagged vesicle proteins are less well integrated in clusters...
  44. pmc Nanoscopy in a living multicellular organism expressing GFP
    Brian R Rankin
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 100:L63-5. 2011
    ..These results demonstrate that numerous microscopy studies of live samples employing GFP as the marker can be performed at subdiffraction resolution...
  45. pmc Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient
    Christian A Wurm
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 108:13546-51. 2011
    ..We conclude that the nanoscale distribution of the TOM complex is finely adjusted to the cellular conditions, resulting in distribution gradients both within single cells and between adjacent cells...
  46. doi request reprint Carborhodol: a new hybrid fluorophore obtained by combination of fluorescein and carbopyronine dye cores
    Maksim V Sednev
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Bioconjug Chem 24:690-700. 2013
    ..2-1.6 ns) suggests the possibility of dual FLIM with numerous dyes having τ values in the range of 3-5 ns. All of these features make the carborhodol fluorophore a valuable addition to the family of the red-emitting fluorescent dyes...
  47. ncbi request reprint STED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue
    Alf Honigmann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Faraday Discuss 161:77-89; discussion 113-50. 2013
    ....
  48. pmc Solid immersion facilitates fluorescence microscopy with nanometer resolution and sub-ångström emitter localization
    Dominik Wildanger
    Department of NanoBiophotonics, Max Planck Institut for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Adv Mater 24:OP309-13. 2012
    ..4 ± 0.3 nm and with a localization precision of 0.09 nm...
  49. doi request reprint Red-emitting rhodamines with hydroxylated, sulfonated, and phosphorylated dye residues and their use in fluorescence nanoscopy
    Kirill Kolmakov
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemistry 18:12986-98. 2012
    ..All these features, accompanied by a zero net charge in conjugates, were accomplished by the introduction of hydrophilic groups of two types: two hydroxyl groups and one sulfonic acid residue...
  50. doi request reprint STED with wavelengths closer to the emission maximum
    Giuseppe Vicidomini
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 20:5225-36. 2012
    ..The method is exemplified by imaging immunolabeled features in mammalian cells with an up to 3-fold increased STED efficiency compared to that encountered in standard STED nanoscopy implementations...
  51. doi request reprint Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopy
    Kirill Kolmakov
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemistry 16:158-66. 2010
    ..g., amines and thiols) in complex mixtures. High-resolution GSDIM images and live-cell STED-FCS experiments on labeled microtubules and lipids prove the versatility of the novel probes for modern fluorescence microscopy and nanoscopy...
  52. doi request reprint Sharper low-power STED nanoscopy by time gating
    Giuseppe Vicidomini
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
    Nat Methods 8:571-3. 2011
    ..This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells...
  53. ncbi request reprint Rhodamine spiroamides for multicolor single-molecule switching fluorescent nanoscopy
    Vladimir N Belov
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemistry 15:10762-76. 2009
    ..Optical images with resolutions on the nanometer scale illustrate the potential of the labels in the colocalization of biological objects and the two-photon activation technique with optical sectioning...
  54. doi request reprint Three-dimensional stimulated emission depletion microscopy of nitrogen-vacancy centers in diamond using continuous-wave light
    Kyu Young Han
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nano Lett 9:3323-9. 2009
    ..Finally, we exemplify the potential of using nanodiamonds containing NV centers as luminescence tags in STED microscopy. Our results offer new experimental avenues in nanooptics, nanotechnology, and the life sciences...
  55. ncbi request reprint Photostable, amino reactive and water-soluble fluorescent labels based on sulfonated rhodamine with a rigidized xanthene fragment
    Vadim P Boyarskiy
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemistry 14:1784-92. 2008
    ..Subdiffraction resolution images of tubulin filaments of mammalian cells stained with these dyes illustrate their applicability as labels for stimulated emission depletion microscopy and other fluorescence techniques...
  56. doi request reprint Triplet-relaxation microscopy with bunched pulsed excitation
    Gerald Donnert
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Photochem Photobiol Sci 8:481-5. 2009
    ..Reaching almost T-Rex conditions this excitation scheme mimics fast scanning of the illumination beam and has the potential to improve a whole range of analytical tools that suffer from photobleaching and low signal levels...
  57. pmc Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids
    Steffen J Sahl
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 107:6829-34. 2010
    ..Our experimental approach demonstrates that fast molecular movements can be tracked with minimal invasion, which can reveal new important details of cellular nano-organization...
  58. doi request reprint Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins
    Flavie Lavoie-Cardinal
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Göttingen Germany, Fax 49 551 201 2505 Equal contributions
    Chemphyschem 15:655-63. 2014
    ....
  59. doi request reprint Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS)
    Marcel Leutenegger
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 20:5243-63. 2012
    ..Together with the estimated axial confinement of about 55 nm, our TIRF-STED nanoscope achieved an almost isotropic and less than 1 attoliter small all-optically induced measurement volume...
  60. pmc Membrane protein sequestering by ionic protein-lipid interactions
    Geert van den Bogaart
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 479:552-5. 2011
    ..Our results demonstrate that electrostatic protein-lipid interactions can result in the formation of microdomains independently of cholesterol or lipid phases...
  61. ncbi request reprint Photoconversion of matrix targeted GFP enables analysis of continuity and intermixing of the mitochondrial lumen
    Stefan Jakobs
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    FEBS Lett 554:194-200. 2003
    ..Matrix constrictions frequently occurring in wild type cells as well as in Deltafis1 and Deltadnm1 mutants do not interfere with luminal continuity...
  62. doi request reprint Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses
    Johanna Bückers
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 19:3130-43. 2011
    ..Furthermore, we propose a setup having a second STED beam for long duration multicolor recording...
  63. doi request reprint Far-field autofluorescence nanoscopy
    Jakob Bierwagen
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gttingen, Germany
    Nano Lett 10:4249-52. 2010
    ..The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts...
  64. doi request reprint Three-dimensional nanoscopy of colloidal crystals
    Benjamin Harke
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nano Lett 8:1309-13. 2008
    ..The mapping of a model system of spheres organized by confined convective assembly unambiguously identified face-centered cubic, hexagonal close-packed, random hexagonal close-packed, and body-centered cubic structures...
  65. doi request reprint A STED microscope aligned by design
    Dominik Wildanger
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 17:16100-10. 2009
    ..The design of a phase plate is described which selectively modulates the STED beam but leaves the excitation beam unaffected. The performance of the single-beam setup is on par with previous dual-beam designs...
  66. doi request reprint A readily retrievable pool of synaptic vesicles
    Yunfeng Hua
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany
    Nat Neurosci 14:833-9. 2011
    ..By using fluorescence nanoscopy of surface-labeled synaptotagmin 1, we could resolve the spatial distribution of the surface pool at the periactive zone in hippocampal boutons, which represent putative sites of endocytosis...
  67. pmc Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell
    Birka Hein
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 105:14271-6. 2008
    ..Time-lapse STED recordings document morphological changes of the ER over time. Thus, nanoscale 3D imaging of organelles in the interior of living cells greatly expands the scope of light microscopy in cell biology...
  68. doi request reprint Masked red-emitting carbopyronine dyes with photosensitive 2-diazo-1-indanone caging group
    Kirill Kolmakov
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Photochem Photobiol Sci 11:522-32. 2012
    ..For use in immunolabelling the caged dyes were decorated with a (hydrophilic) linker and an (activated) carboxyl group...
  69. ncbi request reprint Spatial and temporal dynamics of budding yeast mitochondria lacking the division component Fis1p
    Stefan Jakobs
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Cell Sci 116:2005-14. 2003
    ..The data suggest that different molecular machineries are responsible for the separation of the matrix and the fission of the outer membrane in budding yeast...
  70. doi request reprint Dynamic imaging of colloidal-crystal nanostructures at 200 frames per second
    Marcel A Lauterbach
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37075 Gottingen, Germany
    Langmuir 26:14400-4. 2010
    ..With a temporal resolution of 5 ms, we exemplify the technique by visualizing the annealing of potential point defects during the formation of the colloidal crystal...
  71. doi request reprint Automatic deconvolution of 4Pi-microscopy data with arbitrary phase
    Giuseppe Vicidomini
    MPI for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Lett 34:3583-5. 2009
    ..Taking several 4Pi images at different relative phases of the interfering beams is shown to improve the robustness of the approach...
  72. ncbi request reprint Stimulated-emission-depletion microscopy with a multicolor stimulated-Raman-scattering light source
    Brian R Rankin
    Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Opt Lett 33:2491-3. 2008
    ..This SRS light source enables the simple implementation of multicolor STED and provides a spectral output with multiple available wavelengths from green to red with potential for further expansion...
  73. pmc Structure and mechanism of the reversible photoswitch of a fluorescent protein
    Martin Andresen
    Department of NanoBiophotonics, Theoretical and Computational Biophysics, and X Ray Crystallography, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 102:13070-4. 2005
    ..Reversible photoswitching of the protein chromophore system within intact crystals also constitutes a step toward the use of fluorescent proteins in three-dimensional data recording...
  74. pmc Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins
    Michael Hofmann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 102:17565-9. 2005
    ..Our results underscore the potential to finally achieve molecular resolution in fluorescence microscopy by technical optimization...
  75. pmc Macromolecular-scale resolution in biological fluorescence microscopy
    Gerald Donnert
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 103:11440-5. 2006
    ..The reported performance of diffraction-unlimited fluorescence microscopy opens up a pathway for addressing fundamental problems in the life sciences...
  76. ncbi request reprint 4Pi microscopy of quantum dot-labeled cellular structures
    Rebecca Medda
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37070 Gottingen, Germany
    J Struct Biol 156:517-23. 2006
    ..In particular, we visualize the three-dimensional entanglement of the two networks with unprecedented detail...
  77. ncbi request reprint STED microscopy with a supercontinuum laser source
    Dominik Wildanger
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 16:9614-21. 2008
    ..The imaging of dense nanoparticles and of the microtubular network of mammalian cells evidences a spatial resolution of 30-50 nm in the focal plane, i.e. by a factor of 8-9 beyond the diffraction barrier...
  78. ncbi request reprint Immunofluorescence stimulated emission depletion microscopy
    Marcus Dyba
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Nat Biotechnol 21:1303-4. 2003
    ..We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation...
  79. ncbi request reprint Fluorescence microscopy with super-resolved optical sections
    Alexander Egner
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics 37077 Göttingen, Germany
    Trends Cell Biol 15:207-15. 2005
    ..Noninvasive axial sections of 80-160 nm thickness deliver more faithful 3D-images of subcellular features, providing a new opportunity to significantly enhance our understanding of cellular structure and function...
  80. ncbi request reprint 4Pi-microscopy of the Golgi apparatus in live mammalian cells
    Alexander Egner
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, High Resolution Optical Microscopy Group, 37077 Goettingen, Germany
    J Struct Biol 147:70-6. 2004
    ..Superresolved 3D-fluorescence imaging is exemplified with the first representation of the Golgi apparatus in a live cell at approximately 100 nm resolution...
  81. ncbi request reprint Far-field optical nanoscopy
    Stefan W Hell
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, and German Cancer Research Center DKFZ, High Resolution Optical Microscopy Division, 69120 Heidelberg, Germany
    Science 316:1153-8. 2007
    ..Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization...
  82. pmc Fast 100-nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast
    Alexander Egner
    High Resolution Optical Microscopy Group, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 99:3370-5. 2002
    ..Moreover, this change is associated with a 2.8-fold increase of the surface of the reticulum, resulting in an average increase in volume of the mitochondrial compartment by a factor of 3.0 +/- 0.2...
  83. ncbi request reprint Synthesis and characterization of photoswitchable fluorescent silica nanoparticles
    Jonas Fölling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Small 4:134-42. 2008
    ..Some applications of these particles in fluorescence microscopy are also demonstrated. In particular, subdiffraction images of nanoparticles were obtained, in the focal plane of a confocal microscope...
  84. ncbi request reprint Fluorescence fluctuation spectroscopy in subdiffraction focal volumes
    Lars Kastrup
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Phys Rev Lett 94:178104. 2005
    ..Our method significantly extends the potential of far-field FFS, including for the noninvasive investigation of molecular reactions at higher concentrations...
  85. ncbi request reprint Photostability of a fluorescent marker under pulsed excited-state depletion through stimulated emission
    Marcus Dyba
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Appl Opt 42:5123-9. 2003
    ..One can counteract photobleaching by employing STED pulses that range from 150 ps to approximately half of the lifetime of the excited state. The results also have implications for multiphoton excitation microscopy...
  86. ncbi request reprint 1,3-bicyclo[1.1.1]pentanediyl: the shortest rigid linear connector of phenylated photochromic units and a 1,5-dimethoxy-9,10-di(phenylethynyl)anthracene fluorophore
    Armin de Meijere
    Institut fur Organische und Biomolekulare Chemie, Georg August Universitat Gottingen, Tammannstrasse 2, 37077 Gottingen, Germany
    Chemistry 13:2503-16. 2007
    ..The closed forms of the methoxy-substituted photochromic units 2 and 3 are less resistant to UV light (313 nm) than the closed form of 1...
  87. ncbi request reprint Major signal increase in fluorescence microscopy through dark-state relaxation
    Gerald Donnert
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 4:81-6. 2007
    ..The signal gain was observed both for one- and two-photon excitation. Obeying dark or triplet state relaxation in the illumination process signifies a major step toward imaging with low photobleaching and strong fluorescence fluxes...
  88. ncbi request reprint STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis
    Katrin I Willig
    Departments of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nature 440:935-9. 2006
    ..Our study also demonstrates that questions involving cellular structures with dimensions of a few tens of nanometres can be resolved with conventional far-field optics and visible light...
  89. pmc Two-color far-field fluorescence nanoscopy
    Gerald Donnert
    Biophys J 92:L67-9. 2007
    ..The joint improvement of resolution and colocalization demonstrates the emerging potential of far-field fluorescence nanoscopy to study the spatial organization of macromolecules in cells...
  90. ncbi request reprint Fluorescence nanoscopy goes multicolor
    Andreas Schönle
    Nat Biotechnol 25:1234-5. 2007
  91. pmc Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy
    Hilmar Gugel
    Leica Microsystems Heidelberg GmbH, 68165 Mannheim, Germany
    Biophys J 87:4146-52. 2004
    ..Realized in a state-of-the-art scanning microscope, this approach enables robust three-dimensional imaging of fixed and live cells at approximately 80 nm axial resolution...
  92. ncbi request reprint Fluorescence resonance energy transfer analysis of protein-protein interactions in single living cells by multifocal multiphoton microscopy
    Irina Majoul
    Department of Neurobiology, Max Planck Institute of Biophysical Chemistry, Gottingen, Germany
    J Biotechnol 82:267-77. 2002
    ..Finally, FRET-MMM records performed continuously over 2 h allowed to analyze intracellular retrograde transport and sorting events and to discuss these mechanisms on a single cell level...
  93. ncbi request reprint 2,2'-thiodiethanol: a new water soluble mounting medium for high resolution optical microscopy
    Thorsten Staudt
    German Cancer Research Center Heidelberg, High Resolution Optical Microscopy Division, Heidelberg, Germany
    Microsc Res Tech 70:1-9. 2007
    ..We present the optical and chemical properties of this new medium as well as its application to a variety of differently stained cells and cellular substructures...
  94. ncbi request reprint Regulation of endothelial barrier function during flow-induced conversion to an arterial phenotype
    Jochen Seebach
    Institute of Physiology, Medical Faculty Dresden of the TU Dresden, 01307 Dresden, Germany
    Cardiovasc Res 75:596-607. 2007
    ..Flow-induced conversion of endothelial cells into an elongated arterial phenotype requires a coordinated regulation of cell junctions. Here we investigated the effect of acute and chronic flow on junction regulation...
  95. ncbi request reprint 4Pi microscopy with linear fluorescence excitation
    Marion C Lang
    High Resolution Optical Microscopy Division, German Cancer Research Center DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Opt Lett 32:259-61. 2007
    ..An axial resolution of 95 nm, corresponding to a more than fourfold improvement over confocal microscopy, is verified in the imaging of microtubules in mammalian cells...