S W Hell

Summary

Country: Germany

Publications

  1. pmc Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms
    Daniel Neumann
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    PMC Biophys 3:4. 2010
  2. ncbi request reprint Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range
    S W Hell
    Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Microsc 187:1-7. 1997
  3. doi request reprint Molecular orientation affects localization accuracy in superresolution far-field fluorescence microscopy
    Johann Engelhardt
    German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Nano Lett 11:209-13. 2011
  4. ncbi request reprint Far-field optical nanoscopy
    Stefan W Hell
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, and German Cancer Research Center DKFZ, High Resolution Optical Microscopy Division, 69120 Heidelberg, Germany
    Science 316:1153-8. 2007
  5. doi request reprint Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 26:1035-40. 2008
  6. pmc STED nanoscopy reveals molecular details of cholesterol- and cytoskeleton-modulated lipid interactions in living cells
    V Mueller
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 101:1651-60. 2011
  7. ncbi request reprint EFGP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy
    S Jakobs
    Max Planck Institute for Biophysicial Chemistry, High Resolution Optical Microscopy Group, Gottingen, Germany
    FEBS Lett 479:131-5. 2000
  8. ncbi request reprint Anatomy and dynamics of a supramolecular membrane protein cluster
    Jochen J Sieber
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Science 317:1072-6. 2007
  9. ncbi request reprint Live cell imaging by multifocal multiphoton microscopy
    M Straub
    High Resolution Optical Microscopy Group, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Eur J Cell Biol 79:726-34. 2000
  10. ncbi request reprint Reversible photoswitching enables single-molecule fluorescence fluctuation spectroscopy at high molecular concentration
    C Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Microsc Res Tech 70:1003-9. 2007

Collaborators

Detail Information

Publications80

  1. pmc Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms
    Daniel Neumann
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    PMC Biophys 3:4. 2010
    ..pacs: 87.16.tb, 87.85.rs...
  2. ncbi request reprint Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range
    S W Hell
    Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Microsc 187:1-7. 1997
    ..A comparison with unrestored two-photon confocal images reveals a total reduction of the uncertainty volume up to a factor of 15...
  3. doi request reprint Molecular orientation affects localization accuracy in superresolution far-field fluorescence microscopy
    Johann Engelhardt
    German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Nano Lett 11:209-13. 2011
    ..When imaging rotation-impaired fluorophores of unknown random orientation, the average localization accuracy in three-dimensional samples is typically limited to about ±32 nm, restricting the attainable resolution accordingly...
  4. ncbi request reprint Far-field optical nanoscopy
    Stefan W Hell
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, and German Cancer Research Center DKFZ, High Resolution Optical Microscopy Division, 69120 Heidelberg, Germany
    Science 316:1153-8. 2007
    ..Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization...
  5. doi request reprint Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 26:1035-40. 2008
    ..Furthermore, we demonstrate dual-color fluorescence microscopy with sub-diffraction resolution using bsDronpa and Dronpa whose emission maxima are separated by <20 nm...
  6. pmc STED nanoscopy reveals molecular details of cholesterol- and cytoskeleton-modulated lipid interactions in living cells
    V Mueller
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 101:1651-60. 2011
    ..This strong interaction is different from that responsible for forming cholesterol-dependent, liquid-ordered domains in model membranes...
  7. ncbi request reprint EFGP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy
    S Jakobs
    Max Planck Institute for Biophysicial Chemistry, High Resolution Optical Microscopy Group, Gottingen, Germany
    FEBS Lett 479:131-5. 2000
    ..In aging bacterial cultures DsRed appeared to aggregate within the cells, accompanied by a strong reduction in its fluorescence lifetime as determined by fluorescence lifetime imaging microscopy...
  8. ncbi request reprint Anatomy and dynamics of a supramolecular membrane protein cluster
    Jochen J Sieber
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Science 317:1072-6. 2007
    ....
  9. ncbi request reprint Live cell imaging by multifocal multiphoton microscopy
    M Straub
    High Resolution Optical Microscopy Group, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Eur J Cell Biol 79:726-34. 2000
    ..We conclude that MMM proves to be a technically simple and very effective method for fast 3-D live cell imaging at high resolution...
  10. ncbi request reprint Reversible photoswitching enables single-molecule fluorescence fluctuation spectroscopy at high molecular concentration
    C Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Microsc Res Tech 70:1003-9. 2007
    ..Photoswitching expands the range of single-molecule detection based experiments such as fluorescence fluctuation spectroscopy to large entity concentrations in the micromolar range...
  11. doi request reprint Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers
    Jonas Fölling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemphyschem 9:321-6. 2008
    ..Future synthesis of similar compounds holds great promise for cost-effective fluorescence nanoscopy with noninvasive optical sectioning...
  12. pmc Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy
    Andre C Stiel
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 95:2989-97. 2008
    ..We demonstrate time-lapse live-cell subdiffraction microscopy by imaging rsCherryRev targeted to the endoplasmic reticulum utilizing the switching and localization of single molecules...
  13. pmc Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters
    Alexander Egner
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Biophys J 93:3285-90. 2007
    ..These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm...
  14. ncbi request reprint Photoconversion of matrix targeted GFP enables analysis of continuity and intermixing of the mitochondrial lumen
    Stefan Jakobs
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    FEBS Lett 554:194-200. 2003
    ..Matrix constrictions frequently occurring in wild type cells as well as in Deltafis1 and Deltadnm1 mutants do not interfere with luminal continuity...
  15. ncbi request reprint Molecular organization of an amphiphilic styryl pyridinium dye in monolayers at the air/water interface in the presence of various anions
    Andrey A Turshatov
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Langmuir 22:1571-9. 2006
    ..Theoretical calculations did not yield a transition in the observed range, even for large aggregates (J aggregates). Therefore, other interactions may be responsible for the appearance of this band...
  16. doi request reprint Direct observation of the nanoscale dynamics of membrane lipids in a living cell
    Christian Eggeling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 457:1159-62. 2009
    ..The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells...
  17. doi request reprint Triplet-relaxation microscopy with bunched pulsed excitation
    Gerald Donnert
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Photochem Photobiol Sci 8:481-5. 2009
    ..Reaching almost T-Rex conditions this excitation scheme mimics fast scanning of the illumination beam and has the potential to improve a whole range of analytical tools that suffer from photobleaching and low signal levels...
  18. doi request reprint Rhodamine spiroamides for multicolor single-molecule switching fluorescent nanoscopy
    Vladimir N Belov
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemistry 15:10762-76. 2009
    ..Optical images with resolutions on the nanometer scale illustrate the potential of the labels in the colocalization of biological objects and the two-photon activation technique with optical sectioning...
  19. doi request reprint Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopy
    Kirill Kolmakov
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemistry 16:158-66. 2010
    ..g., amines and thiols) in complex mixtures. High-resolution GSDIM images and live-cell STED-FCS experiments on labeled microtubules and lipids prove the versatility of the novel probes for modern fluorescence microscopy and nanoscopy...
  20. ncbi request reprint Fluorescence resonance energy transfer analysis of protein-protein interactions in single living cells by multifocal multiphoton microscopy
    Irina Majoul
    Department of Neurobiology, Max Planck Institute of Biophysical Chemistry, Gottingen, Germany
    J Biotechnol 82:267-77. 2002
    ..Finally, FRET-MMM records performed continuously over 2 h allowed to analyze intracellular retrograde transport and sorting events and to discuss these mechanisms on a single cell level...
  21. ncbi request reprint Influence of monolayer state on spectroscopy and photoisomerization of an amphiphilic styryl-pyridinium dye on a solid substrate
    Mariano L Bossi
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Langmuir 23:3699-705. 2007
    ....
  22. doi request reprint A compact STED microscope providing 3D nanoscale resolution
    D Wildanger
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
    J Microsc 236:35-43. 2009
    ..The obtained results can be further improved by mathematical restoration algorithms. The far-field optical nanoscale resolution is attained in a variety of biological samples featuring strong variations in the local density of features...
  23. pmc Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins
    Michael Hofmann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 102:17565-9. 2005
    ..Our results underscore the potential to finally achieve molecular resolution in fluorescence microscopy by technical optimization...
  24. ncbi request reprint Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching
    Miriam A Schwentker
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Microsc Res Tech 70:269-80. 2007
    ..The obtained resolution of 50 nm ( approximately lambda/12) is limited only by the spectroscopic properties of the proteins and the imperfections of the optical implementation, but not on principle grounds...
  25. pmc Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell
    Birka Hein
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 105:14271-6. 2008
    ..Time-lapse STED recordings document morphological changes of the ER over time. Thus, nanoscale 3D imaging of organelles in the interior of living cells greatly expands the scope of light microscopy in cell biology...
  26. doi request reprint Enhancing fluorescence brightness: effect of reverse intersystem crossing studied by fluorescence fluctuation spectroscopy
    Christian Ringemann
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Chemphyschem 9:612-24. 2008
    ..The study of ReISC not only results in a better understanding of a fluorescent label's photophysics, but the method is a possible approach to optimize fluorescence emission in experiments, where signal strength is a critical parameter...
  27. doi request reprint Mitochondrial cristae revealed with focused light
    Roman Schmidt
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37077 Gottingen, Germany
    Nano Lett 9:2508-10. 2009
    ..We find a pronounced heterogeneity in the cristae arrangements even within individual mitochondrial tubules...
  28. ncbi request reprint Spatial and temporal dynamics of budding yeast mitochondria lacking the division component Fis1p
    Stefan Jakobs
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Cell Sci 116:2005-14. 2003
    ..The data suggest that different molecular machineries are responsible for the separation of the matrix and the fission of the outer membrane in budding yeast...
  29. pmc Stimulated emission depletion nanoscopy of living cells using SNAP-tag fusion proteins
    Birka Hein
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
    Biophys J 98:158-63. 2010
    ..Hence fusion proteins that bind modified organic dyes expand widely the application range of far-field fluorescence nanoscopy of living cells...
  30. ncbi request reprint Two-photon excitation STED microscopy
    Gael Moneron
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 17:14567-73. 2009
    ..4-fold improvement over the diffraction barrier...
  31. ncbi request reprint STED microscopy with continuous wave beams
    Katrin I Willig
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 4:915-8. 2007
    ..Viable for fluorophores with low triplet yield, the use of CW light sources greatly simplifies the implementation of this concept of far-field fluorescence nanoscopy...
  32. ncbi request reprint Photostable, amino reactive and water-soluble fluorescent labels based on sulfonated rhodamine with a rigidized xanthene fragment
    Vadim P Boyarskiy
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemistry 14:1784-92. 2008
    ..Subdiffraction resolution images of tubulin filaments of mammalian cells stained with these dyes illustrate their applicability as labels for stimulated emission depletion microscopy and other fluorescence techniques...
  33. ncbi request reprint Synthesis and characterization of photoswitchable fluorescent silica nanoparticles
    Jonas Fölling
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Small 4:134-42. 2008
    ..Some applications of these particles in fluorescence microscopy are also demonstrated. In particular, subdiffraction images of nanoparticles were obtained, in the focal plane of a confocal microscope...
  34. doi request reprint Video-rate far-field optical nanoscopy dissects synaptic vesicle movement
    Volker Westphal
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen 37077, Germany
    Science 320:246-9. 2008
    ..Our study demonstrates the emerging ability of optical microscopy to investigate intracellular physiological processes on the nanoscale in real time...
  35. doi request reprint Spherical nanosized focal spot unravels the interior of cells
    Roman Schmidt
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 5:539-44. 2008
    ..Fully relying on focused light, this lens-based fluorescence nanoscope unravels the interior of cells noninvasively, uniquely dissecting their sub-lambda-sized organelles...
  36. doi request reprint Dual-color STED microscopy at 30-nm focal-plane resolution
    Lars Meyer
    Max Planck Institute for Biophysical Chemistry, Dept of NanoBiophotonics, 37070 Gottingen, Germany
    Small 4:1095-100. 2008
  37. pmc Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron
    Tanja Brakemann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Biol Chem 285:14603-9. 2010
    ..Distinct absorption cross-sections for the switching wavelengths in the fluorescent and the nonfluorescent state are not essential for efficient photochromism in fluorescent proteins, although they may facilitate the switching process...
  38. ncbi request reprint Major signal increase in fluorescence microscopy through dark-state relaxation
    Gerald Donnert
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 4:81-6. 2007
    ..The signal gain was observed both for one- and two-photon excitation. Obeying dark or triplet state relaxation in the illumination process signifies a major step toward imaging with low photobleaching and strong fluorescence fluxes...
  39. doi request reprint Three-dimensional stimulated emission depletion microscopy of nitrogen-vacancy centers in diamond using continuous-wave light
    Kyu Young Han
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nano Lett 9:3323-9. 2009
    ..Finally, we exemplify the potential of using nanodiamonds containing NV centers as luminescence tags in STED microscopy. Our results offer new experimental avenues in nanooptics, nanotechnology, and the life sciences...
  40. pmc Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids
    Steffen J Sahl
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 107:6829-34. 2010
    ..Our experimental approach demonstrates that fast molecular movements can be tracked with minimal invasion, which can reveal new important details of cellular nano-organization...
  41. ncbi request reprint Nanoscale resolution in GFP-based microscopy
    Katrin I Willig
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 3:721-3. 2006
    ..Our results mark the advent of nanoscale biological microscopy with genetically encoded markers...
  42. doi request reprint Fluorescence nanoscopy by ground-state depletion and single-molecule return
    Jonas Fölling
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Methods 5:943-5. 2008
    ..Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach...
  43. ncbi request reprint Nanoscale organization of nicotinic acetylcholine receptors revealed by stimulated emission depletion microscopy
    R R Kellner
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37077 Gottingen, Germany
    Neuroscience 144:135-43. 2007
    ..5-3.5 microm), which possibly are related to the abolition of cytoskeletal physical barriers preventing the lateral diffusion of AChR nanoclusters...
  44. ncbi request reprint Comparison of I5M and 4Pi-microscopy
    J Bewersdorf
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Gottingen, Germany
    J Microsc 222:105-17. 2006
    ..In conclusion, with the current aperture angles and fluorescence signal strengths, it is not advisable to trade in the suppression of the sidelobes for a larger image signal...
  45. ncbi request reprint KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET
    I Majoul
    Department of Neurobiology, Max Planck Institute of Biophysical Chemistry, Gottingen, Germany
    Dev Cell 1:139-53. 2001
    ..Both p24a and p23 interact with ARF1, but only p24 interacts with ARFGAP. These findings suggest a model for how cargo-induced oligomerization of ERD2 regulates its sorting into COPI-coated buds...
  46. pmc 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants
    Andre C Stiel
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Biochem J 402:35-42. 2007
    ..The findings reported in the present study support the view that a cis-trans isomerization is one of the key events common to the switching mechanism in RSFPs...
  47. ncbi request reprint 4Pi-microscopy of the Golgi apparatus in live mammalian cells
    Alexander Egner
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, High Resolution Optical Microscopy Group, 37077 Goettingen, Germany
    J Struct Biol 147:70-6. 2004
    ..Superresolved 3D-fluorescence imaging is exemplified with the first representation of the Golgi apparatus in a live cell at approximately 100 nm resolution...
  48. ncbi request reprint Nanoscale separation of molecular species based on their rotational mobility
    Ilaria Testa
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 16:21093-104. 2008
    ..Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells...
  49. ncbi request reprint Concepts for nanoscale resolution in fluorescence microscopy
    Stefan W Hell
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37070 Gottingen, Germany
    Curr Opin Neurobiol 14:599-609. 2004
    ..Formed on the basis of reversible saturable optical transitions, these concepts might eventually allow us to investigate hitherto inaccessible details within live cells...
  50. doi request reprint Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
    Mariano Bossi
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Nano Lett 8:2463-8. 2008
    ..The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques...
  51. pmc Myelin basic protein-dependent plasma membrane reorganization in the formation of myelin
    Dirk Fitzner
    Centre for Biochemistry and Molecular Cell Biology, University of Gottingen, Gottingen, Germany
    EMBO J 25:5037-48. 2006
    ..We propose that this mechanism is essential for myelin to perform its insulating function during nerve conduction...
  52. pmc Structure and mechanism of the reversible photoswitch of a fluorescent protein
    Martin Andresen
    Department of NanoBiophotonics, Theoretical and Computational Biophysics, and X Ray Crystallography, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 102:13070-4. 2005
    ..Reversible photoswitching of the protein chromophore system within intact crystals also constitutes a step toward the use of fluorescent proteins in three-dimensional data recording...
  53. ncbi request reprint 2,2'-thiodiethanol: a new water soluble mounting medium for high resolution optical microscopy
    Thorsten Staudt
    German Cancer Research Center Heidelberg, High Resolution Optical Microscopy Division, Heidelberg, Germany
    Microsc Res Tech 70:1-9. 2007
    ..We present the optical and chemical properties of this new medium as well as its application to a variety of differently stained cells and cellular substructures...
  54. ncbi request reprint Fluorescence fluctuation spectroscopy in subdiffraction focal volumes
    Lars Kastrup
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Phys Rev Lett 94:178104. 2005
    ..Our method significantly extends the potential of far-field FFS, including for the noninvasive investigation of molecular reactions at higher concentrations...
  55. pmc Structural basis for reversible photoswitching in Dronpa
    Martin Andresen
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 104:13005-9. 2007
    ..We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore...
  56. doi request reprint Comparing video-rate STED nanoscopy and confocal microscopy of living neurons
    Marcel A Lauterbach
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Biophotonics 3:417-24. 2010
    ..Tentatively providing a larger photon flux, CW beams should facilitate extending fast STED imaging towards imaging fainter living samples...
  57. ncbi request reprint Photostability of a fluorescent marker under pulsed excited-state depletion through stimulated emission
    Marcus Dyba
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Appl Opt 42:5123-9. 2003
    ..One can counteract photobleaching by employing STED pulses that range from 150 ps to approximately half of the lifetime of the excited state. The results also have implications for multiphoton excitation microscopy...
  58. ncbi request reprint Toward fluorescence nanoscopy
    Stefan W Hell
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gottingen, Germany
    Nat Biotechnol 21:1347-55. 2003
    ..Relying on saturated optical transitions, these concepts are limited only by the attainable saturation level. As strong saturation should be feasible at low light intensities, nanoscale imaging with focused light may be closer than ever...
  59. pmc Fast 100-nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast
    Alexander Egner
    High Resolution Optical Microscopy Group, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 99:3370-5. 2002
    ..Moreover, this change is associated with a 2.8-fold increase of the surface of the reticulum, resulting in an average increase in volume of the mitochondrial compartment by a factor of 3.0 +/- 0.2...
  60. ncbi request reprint Breaking the diffraction barrier in fluorescence microscopy by optical shelving
    Stefan Bretschneider
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Phys Rev Lett 98:218103. 2007
    ..The presence of dark states in virtually any fluorescent molecule opens up a new venue for far-field microscopy with resolution that is no longer limited by diffraction...
  61. ncbi request reprint A new high-aperture glycerol immersion objective lens and its application to 3D-fluorescence microscopy
    N Martini
    High Resolution Optical Microscopy Group, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Microsc 206:146-51. 2002
    ..The unique advantages of these new lenses in high-resolution microscopy with two coherently used opposing lenses, such as 4 Pi-microscopy, are discussed...
  62. ncbi request reprint 4Pi microscopy of quantum dot-labeled cellular structures
    Rebecca Medda
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37070 Gottingen, Germany
    J Struct Biol 156:517-23. 2006
    ..In particular, we visualize the three-dimensional entanglement of the two networks with unprecedented detail...
  63. pmc High- and low-mobility stages in the synaptic vesicle cycle
    Dirk Kamin
    STED Microscopy of Synaptic Function, European Neuroscience Institute, Gottingen, Germany
    Biophys J 99:675-84. 2010
    ..We conclude that both high- and low-mobility states are characteristic of synaptic vesicle movement...
  64. pmc Macromolecular-scale resolution in biological fluorescence microscopy
    Gerald Donnert
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 103:11440-5. 2006
    ..The reported performance of diffraction-unlimited fluorescence microscopy opens up a pathway for addressing fundamental problems in the life sciences...
  65. doi request reprint Microscopy and its focal switch
    Stefan W Hell
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37070 Gottingen, Germany
    Nat Methods 6:24-32. 2009
    ..Here I discuss the principles of these methods together with their differences in implementation and operation. Finally, I outline potential developments...
  66. doi request reprint Three-dimensional nanoscopy of colloidal crystals
    Benjamin Harke
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nano Lett 8:1309-13. 2008
    ..The mapping of a model system of spheres organized by confined convective assembly unambiguously identified face-centered cubic, hexagonal close-packed, random hexagonal close-packed, and body-centered cubic structures...
  67. doi request reprint Direct light-driven modulation of luminescence from Mn-doped ZnSe quantum dots
    Scott E Irvine
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Angew Chem Int Ed Engl 47:2685-8. 2008
  68. ncbi request reprint STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis
    Katrin I Willig
    Departments of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Nature 440:935-9. 2006
    ..Our study also demonstrates that questions involving cellular structures with dimensions of a few tens of nanometres can be resolved with conventional far-field optics and visible light...
  69. doi request reprint 3D reconstruction of high-resolution STED microscope images
    Annedore Punge
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Microsc Res Tech 71:644-50. 2008
    ..This approach allows a 3D image reconstruction with a resolution <80 nm in all directions using available state-of-the art STED microscopes...
  70. ncbi request reprint Resolution scaling in STED microscopy
    Benjamin Harke
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 16:4154-62. 2008
    ..For relevant saturation values, the generalized square-root law is shown to predict the practical resolution with high accuracy...
  71. doi request reprint Fast STED microscopy with continuous wave fiber lasers
    Gael Moneron
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Opt Express 18:1302-9. 2010
    ..2 s...
  72. ncbi request reprint Fluorescence nanoscopy goes multicolor
    Andreas Schönle
    Nat Biotechnol 25:1234-5. 2007
  73. ncbi request reprint STED microscopy with a supercontinuum laser source
    Dominik Wildanger
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Opt Express 16:9614-21. 2008
    ..The imaging of dense nanoparticles and of the microtubular network of mammalian cells evidences a spatial resolution of 30-50 nm in the focal plane, i.e. by a factor of 8-9 beyond the diffraction barrier...
  74. pmc The SNARE motif is essential for the formation of syntaxin clusters in the plasma membrane
    Jochen J Sieber
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Biophys J 90:2843-51. 2006
    ..Thus, syntaxin clustering represents a mechanism of membrane patterning that is based on protein-protein interactions...
  75. ncbi request reprint Regulation of endothelial barrier function during flow-induced conversion to an arterial phenotype
    Jochen Seebach
    Institute of Physiology, Medical Faculty Dresden of the TU Dresden, 01307 Dresden, Germany
    Cardiovasc Res 75:596-607. 2007
    ..Flow-induced conversion of endothelial cells into an elongated arterial phenotype requires a coordinated regulation of cell junctions. Here we investigated the effect of acute and chronic flow on junction regulation...
  76. ncbi request reprint Immunofluorescence stimulated emission depletion microscopy
    Marcus Dyba
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Nat Biotechnol 21:1303-4. 2003
    ..We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation...
  77. ncbi request reprint Fluorescence microscopy with super-resolved optical sections
    Alexander Egner
    Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics 37077 Göttingen, Germany
    Trends Cell Biol 15:207-15. 2005
    ..Noninvasive axial sections of 80-160 nm thickness deliver more faithful 3D-images of subcellular features, providing a new opportunity to significantly enhance our understanding of cellular structure and function...
  78. pmc Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy
    Hilmar Gugel
    Leica Microsystems Heidelberg GmbH, 68165 Mannheim, Germany
    Biophys J 87:4146-52. 2004
    ..Realized in a state-of-the-art scanning microscope, this approach enables robust three-dimensional imaging of fixed and live cells at approximately 80 nm axial resolution...
  79. pmc Two-color far-field fluorescence nanoscopy
    Gerald Donnert
    Biophys J 92:L67-9. 2007
    ..The joint improvement of resolution and colocalization demonstrates the emerging potential of far-field fluorescence nanoscopy to study the spatial organization of macromolecules in cells...
  80. ncbi request reprint 1,3-bicyclo[1.1.1]pentanediyl: the shortest rigid linear connector of phenylated photochromic units and a 1,5-dimethoxy-9,10-di(phenylethynyl)anthracene fluorophore
    Armin de Meijere
    Institut fur Organische und Biomolekulare Chemie, Georg August Universitat Gottingen, Tammannstrasse 2, 37077 Gottingen, Germany
    Chemistry 13:2503-16. 2007
    ..The closed forms of the methoxy-substituted photochromic units 2 and 3 are less resistant to UV light (313 nm) than the closed form of 1...