R Heintzmann

Summary

Country: Germany

Publications

  1. ncbi Reconstruction of axial tomographic high resolution data from confocal fluorescence microscopy: a method for improving 3D FISH images
    R Heintzmann
    Applied Optics and Information Processing, Institute of Applied Physics, University of Heidelberg, Germany
    Anal Cell Pathol 20:7-15. 2000
  2. ncbi Axial tomographic confocal fluorescence microscopy
    R Heintzmann
    Applied Optics and Information Processing, Kirchhoff Institute of Physics, University of Heidelberg, Albert Ueberle Str 3 5, 69120 Heidelberg, Germany
    J Microsc 206:7-23. 2002
  3. ncbi Saturated patterned excitation microscopy--a concept for optical resolution improvement
    Rainer Heintzmann
    Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Opt Soc Am A Opt Image Sci Vis 19:1599-609. 2002
  4. ncbi Saturated patterned excitation microscopy with two-dimensional excitation patterns
    Rainer Heintzmann
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Göttingen 37077, Germany
    Micron 34:283-91. 2003
  5. ncbi Resolution enhancement by subtraction of confocal signals taken at different pinhole sizes
    Rainer Heintzmann
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Micron 34:293-300. 2003
  6. ncbi Spatially modulated illumination microscopy: online visualization of intensity distribution and prediction of nanometer precision of axial distance measurements by computer simulations
    B Albrecht
    Kirchhoff Institute for Physics (KIP, University of Heidelberg, Applied Optics and Information Processing, D-69120 Heidelberg, Germany
    J Biomed Opt 6:292-9. 2001
  7. ncbi A dual path programmable array microscope (PAM): simultaneous acquisition of conjugate and non-conjugate images
    R Heintzmann
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, , Germany
    J Microsc 204:119-35. 2001
  8. ncbi Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET)
    D S Lidke
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, , Germany
    Biochem Soc Trans 31:1020-7. 2003
  9. ncbi Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction
    Diane S Lidke
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Nat Biotechnol 22:198-203. 2004
  10. ncbi Dynamic speckle illumination microscopy with wavelet prefiltering
    Cathie Ventalon
    Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215, USA
    Opt Lett 32:1417-9. 2007

Collaborators

Detail Information

Publications19

  1. ncbi Reconstruction of axial tomographic high resolution data from confocal fluorescence microscopy: a method for improving 3D FISH images
    R Heintzmann
    Applied Optics and Information Processing, Institute of Applied Physics, University of Heidelberg, Germany
    Anal Cell Pathol 20:7-15. 2000
    ..htm. At this web address an interactive 3D viewer is additionally provided for browsing the 3D data. This java applet displays three orthogonal slices of the data set which are dynamically updated by user mouse clicks or keystrokes...
  2. ncbi Axial tomographic confocal fluorescence microscopy
    R Heintzmann
    Applied Optics and Information Processing, Kirchhoff Institute of Physics, University of Heidelberg, Albert Ueberle Str 3 5, 69120 Heidelberg, Germany
    J Microsc 206:7-23. 2002
    ..A clearly improved 3D resolution was obtained by axial tomography together with reconstruction as compared with reconstruction of confocal data from only a single angular view...
  3. ncbi Saturated patterned excitation microscopy--a concept for optical resolution improvement
    Rainer Heintzmann
    Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    J Opt Soc Am A Opt Image Sci Vis 19:1599-609. 2002
    ..The effects of photon noise are included in the simulations...
  4. ncbi Saturated patterned excitation microscopy with two-dimensional excitation patterns
    Rainer Heintzmann
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Göttingen 37077, Germany
    Micron 34:283-91. 2003
    ..For higher resolution, an increased number of detected photons and of raw data images are required. A potential method for substantially decreasing the required number of raw images in PEM and SPEM is discussed...
  5. ncbi Resolution enhancement by subtraction of confocal signals taken at different pinhole sizes
    Rainer Heintzmann
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Micron 34:293-300. 2003
    ..A comparison of 2D and 3D experimental data analysed with subtractive imaging, the equivalent Fourier-space processing of the confocal data only, and Fourier-space weighted averaging is presented...
  6. ncbi Spatially modulated illumination microscopy: online visualization of intensity distribution and prediction of nanometer precision of axial distance measurements by computer simulations
    B Albrecht
    Kirchhoff Institute for Physics (KIP, University of Heidelberg, Applied Optics and Information Processing, D-69120 Heidelberg, Germany
    J Biomed Opt 6:292-9. 2001
    ....
  7. ncbi A dual path programmable array microscope (PAM): simultaneous acquisition of conjugate and non-conjugate images
    R Heintzmann
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, , Germany
    J Microsc 204:119-35. 2001
    ..We also investigated the properties of images obtained by subjecting the Ic and Inc data to a combined maximum likelihood deconvolution...
  8. ncbi Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET)
    D S Lidke
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, , Germany
    Biochem Soc Trans 31:1020-7. 2003
    ..They are particularly applicable to probes generated by fusions of visible fluorescence proteins, as exemplified by studies of the erbB receptor tyrosine kinases involved in growth-factor-mediated signal transduction...
  9. ncbi Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction
    Diane S Lidke
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Nat Biotechnol 22:198-203. 2004
    ..QD-ligands will find widespread use in basic research and biotechnological developments...
  10. ncbi Dynamic speckle illumination microscopy with wavelet prefiltering
    Cathie Ventalon
    Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215, USA
    Opt Lett 32:1417-9. 2007
    ..The resultant gain in sectioning strength leads to a fundamentally improved scaling law for the out-of-focus background rejection...
  11. ncbi Fluorescence lifetime imaging in an optically sectioning programmable array microscope (PAM)
    Quentin S Hanley
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    Cytometry A 67:112-8. 2005
    ..The PAM can be used in sectioning and nonsectioning modes, thereby constituting a useful platform for fluorescence lifetime imaging...
  12. ncbi Breaking the resolution limit in light microscopy
    Rainer Heintzmann
    Brief Funct Genomic Proteomic 5:289-301. 2006
    ..Key concepts such as the point spread function and the Abbe limit, which are necessary for an in depth understanding of the presented methods, are described without requiring extensive mathematical training...
  13. ncbi A novel site of action for alpha-SNAP in the SNARE conformational cycle controlling membrane fusion
    Marcin Barszczewski
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Biol Cell 19:776-84. 2008
    ..We conclude that alpha-SNAP inhibits exocytosis by binding to the syntaxin SNARE motif and in turn prevents SNARE assembly, revealing an unexpected site of action for alpha-SNAP in the SNARE cycle that drives exocytotic membrane fusion...
  14. ncbi Determinants of synaptobrevin regulation in membranes
    Tabrez J Siddiqui
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Biol Cell 18:2037-46. 2007
    ....
  15. ncbi High-resolution image reconstruction in fluorescence microscopy with patterned excitation
    Rainer Heintzmann
    Randall Division of Cell and Molecular Biophysics, Kings College London, London SE1 1UL, UK
    Appl Opt 45:5037-45. 2006
    ..We investigate the influence of some data processing strategies on unwanted effects such as residual patterning and local deviations from linearity in the reconstructed intensity...
  16. ncbi The SNARE motif is essential for the formation of syntaxin clusters in the plasma membrane
    Jochen J Sieber
    Department of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Biophys J 90:2843-51. 2006
    ..Thus, syntaxin clustering represents a mechanism of membrane patterning that is based on protein-protein interactions...
  17. ncbi Polycomb group protein complexes exchange rapidly in living Drosophila
    Gabriella Ficz
    Max Planck Institute for Biophysical Chemistry, Department of Molecular Biology, 37070 Gottingen, Germany
    Development 132:3963-76. 2005
    ....
  18. ncbi New effects in polynucleotide release from cationic lipid carriers revealed by confocal imaging, fluorescence cross-correlation spectroscopy and single particle tracking
    Svitlana Berezhna
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Goettingen, Germany
    Biochim Biophys Acta 1669:193-207. 2005
    ....
  19. ncbi Three-dimensional FRET reconstruction microscopy for analysis of dynamic molecular interactions in live cells
    Adam D Hoppe
    Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA
    Biophys J 95:400-18. 2008
    ..These results verify previous observations that Cdc42 signaling is localized to the advancing margins of forming phagosomes in macrophages...