James Adjaye

Summary

Affiliation: Heinrich Heine University
Country: Germany

Publications

  1. Spitzhorn L, Megges M, Wruck W, Rahman M, Otte J, Degistirici O, et al. Human iPSC-derived MSCs (iMSCs) from aged individuals acquire a rejuvenation signature. Stem Cell Res Ther. 2019;10:100 pubmed publisher
    ..The iMSC concept could allow circumventing the drawbacks associated with the use of adult MSCs und thus provide a promising tool for use in various clinical settings in the future. ..
  2. Martins S, Bohndorf M, Graffmann N, Wruck W, Chrzanowska K, Adjaye J. Fibroblast-derived integration-free iPSC line ISRM-NBS1 from an 18-year-old Nijmegen Breakage Syndrome patient carrying the homozygous NBN c.657_661del5 mutation. Stem Cell Res. 2019;34:101372 pubmed publisher
    ..955. ..
  3. Spitzhorn L, Kordes C, Megges M, Sawitza I, Götze S, Reichert D, et al. Transplanted human pluripotent stem cell-derived mesenchymal stem cells support liver regeneration in Gunn rats. Stem Cells Dev. 2018;: pubmed publisher
    ..These findings imply that transplanted human iMSCs can contribute to liver regeneration in vivo and thus represent a promising tool for the treatment of inherited liver diseases. ..
  4. Graffmann N, Bohndorf M, Ncube A, Kawala M, Wruck W, Kashofer K, et al. Establishment and characterization of an iPSC line from a 35 years old high grade patient with nonalcoholic fatty liver disease (30-40% steatosis) with homozygous wildtype PNPLA3 genotype. Stem Cell Res. 2018;31:113-116 pubmed publisher
    ..The induced pluripotent stem cells (iPSCs) were characterized by immunocytochemistry, flow cytometry, embryoid body formation, pluritest, DNA-fingerprinting and karyotype analysis. ..
  5. Graffmann N, Bohndorf M, Ncube A, Wruck W, Kashofer K, Zatloukal K, et al. Establishment and characterization of an iPSC line from a 58 years old high grade patient with nonalcoholic fatty liver disease (70% steatosis) with homozygous wildtype PNPLA3 genotype. Stem Cell Res. 2018;31:131-134 pubmed publisher
    ..We characterized the induced pluripotent stem cells (iPSCs) by immunocytochemistry, flow cytometry, embryoid body formation, pluritest DNA-fingerprinting, and karyotype analysis. ..
  6. Martins S, Yigit H, Bohndorf M, Graffmann N, Fiszl A, Wruck W, et al. Lymphoblast-derived integration-free iPSC line AD-TREM2-1 from a 67year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant. Stem Cell Res. 2018;29:60-63 pubmed publisher
    ..947. ..
  7. Abu Dawud R, Graffmann N, Ferber S, Wruck W, Adjaye J. Pluripotent stem cells: induction and self-renewal. Philos Trans R Soc Lond B Biol Sci. 2018;373: pubmed publisher
    ..We propose these as additional biomarkers defining naive PSCs.This article is part of the theme issue 'Designer human tissue: coming to a lab near you'. ..
  8. Rahman M, Spitzhorn L, Wruck W, Hagenbeck C, Balan P, Graffmann N, et al. The presence of human mesenchymal stem cells of renal origin in amniotic fluid increases with gestational time. Stem Cell Res Ther. 2018;9:113 pubmed publisher
    ..Third-trimester amniotic fluid contains AF-MSCs of renal origin and this novel source of kidney progenitors may have enormous future potentials for disease modeling, renal repair and drug screening. ..
  9. Martins S, Yigit H, Bohndorf M, Graffmann N, Fiszl A, Wruck W, et al. Lymphoblast-derived integration-free iPSC line AD-TREM2-3 from a 74 year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant. Stem Cell Res. 2018;30:141-144 pubmed publisher
    ..940. ..

More Information

Publications27

  1. Schröter F, Sleegers K, Cuyvers E, Bohndorf M, Wruck W, Van Broeckhoven C, et al. Lymphoblast-derived integration-free iPS cell line from a female 67-year-old Alzheimer's disease patient with TREM2 (R47H) missense mutation. Stem Cell Res. 2016;17:553-555 pubmed publisher
    ..961. ..
  2. Kawala M, Bohndorf M, Graffmann N, Wruck W, Zatloukal K, Adjaye J. Characterization of dermal fibroblast-derived iPSCs from a patient with low grade steatosis. Stem Cell Res. 2016;17:547-549 pubmed publisher
    ..iPSCs were characterized by immunocytochemistry, embryonic body-formation, DNA-fingerprint karyotype analysis and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearsons correlation of 0.9251. ..
  3. Lichtner B, Matz P, Adjaye J. Generation of iPSC lines from primary human chorionic villi cells. Stem Cell Res. 2015;15:697-9 pubmed publisher
    ..Pluripotency was confirmed both in vivo and in vitro. The transcriptomes of the CV-derived iPSC lines and the human embryonic stem cell lines-H1 and H9 have a Pearson correlation of 0.929 and 0.943 respectively. ..
  4. Mlody B, Adjaye J. Generation of iPSC lines from a Nijmegen Breakage Syndrome patient. Stem Cell Res. 2015;15:629-32 pubmed publisher
    ..Pluripotency was confirmed both in vivo and in vitro. ..
  5. Megges M, Geissler S, Duda G, Adjaye J. Generation of an iPS cell line from bone marrow derived mesenchymal stromal cells from an elderly patient. Stem Cell Res. 2015;15:565-8 pubmed publisher
    ..Pluripotency was confirmed both in vitro and in vivo. ..
  6. Kawala M, Bohndorf M, Graffmann N, Wruck W, Zatloukal K, Adjaye J. Characterization of dermal fibroblast-derived iPSCs from a patient with high grade steatosis. Stem Cell Res. 2016;17:568-571 pubmed publisher
    ..9287. Resource table. ..
  7. Schröter F, Sleegers K, Cuyvers E, Bohndorf M, Wruck W, Van Broeckhoven C, et al. Lymphoblast-derived integration-free iPS cell line from a 65-year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant. Stem Cell Res. 2016;16:113-5 pubmed publisher
    ..966. ..
  8. Drews K, Matz P, Adjaye J. Generation of iPSC lines from primary human amniotic fluid cells. Stem Cell Res. 2015;15:712-4 pubmed publisher
    ..A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human embryonic stem cell lines (H1 and H9) revealed a Pearson correlation of 0.953 and 0.941 respectively. ..
  9. Andersen R, Zaher W, Larsen K, Ditzel N, Drews K, Wruck W, et al. Association between in vivo bone formation and ex vivo migratory capacity of human bone marrow stromal cells. Stem Cell Res Ther. 2015;6:196 pubmed publisher
    ..PDGFRβ can be used as a potential marker for the prospective selection of hBMSC populations with high migration and bone formation capacities suitable for clinical trials for enhancing bone regeneration. ..
  10. Schröter F, Sleegers K, Van Cauwenberghe C, Bohndorf M, Wruck W, Van Broeckhoven C, et al. Lymphoblast-derived integration-free iPSC lines from a female and male Alzheimer's disease patient expressing different copy numbers of a coding CNV in the Alzheimer risk gene CR1. Stem Cell Res. 2016;17:560-563 pubmed publisher
    ..The iPSCs retained the CR1 CNV, and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearson correlation of 0.956 for AD1-CR10 and 0.908 for AD1-CR14. ..
  11. Mertes F, Lichtner B, Kuhl H, Blattner M, Otte J, Wruck W, et al. Combined ultra-low input mRNA and whole-genome sequencing of human embryonic stem cells. BMC Genomics. 2015;16:925 pubmed publisher
    ..The results we present demonstrate that a combined analysis of genomic DNA and messenger RNA from ultra-low input samples is feasible and can readily be applied to other cellular systems with limited material available. ..
  12. Hossini A, Megges M, Prigione A, Lichtner B, Toliat M, Wruck W, et al. Erratum: Induced pluripotent stem cell-derived neuronal cells from a sporadic Alzheimer's disease donor as a model for investigating AD-associated gene regulatory networks. BMC Genomics. 2015;16:433 pubmed publisher
  13. Martins S, Bohndorf M, Schröter F, Assar F, Wruck W, Sleegers K, et al. Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female. Stem Cell Res. 2018;26:76-79 pubmed publisher
    ..95. ..
  14. Bohndorf M, Ncube A, Spitzhorn L, Enczmann J, Wruck W, Adjaye J. Derivation and characterization of integration-free iPSC line ISRM-UM51 derived from SIX2-positive renal cells isolated from urine of an African male expressing the CYP2D6 *4/*17 variant which confers intermediate drug metabolizing activity. Stem Cell Res. 2017;25:18-21 pubmed publisher
    ..Comparative transcriptome analyses with human embryonic stem cell lines H1 and H9 revealed a Pearson correlation of 0.9243 and 0.9619 respectively. ..
  15. Mlody B, Wruck W, Martins S, Sperling K, Adjaye J. Nijmegen Breakage Syndrome fibroblasts and iPSCs: cellular models for uncovering disease-associated signaling pathways and establishing a screening platform for anti-oxidants. Sci Rep. 2017;7:7516 pubmed publisher
    ..Our study demonstrates the utility of NBS-iPSCs as a screening platform for anti-oxidants capable of suppressing DNA damage and a cellular model for studying NBN de-regulation in cancer and microcephaly. ..
  16. Matz P, Wruck W, Fauler B, Herebian D, Mielke T, Adjaye J. Footprint-free human fetal foreskin derived iPSCs: A tool for modeling hepatogenesis associated gene regulatory networks. Sci Rep. 2017;7:6294 pubmed publisher
    ..We use E-iPSCs for modeling gene regulatory networks associated with human hepatogenesis and gastrulation in general. ..
  17. Kawala M, Bohndorf M, Graffmann N, Wruck W, Zatloukal K, Adjaye J. Characterization of iPSCs derived from dermal fibroblasts from a healthy 19year old female. Stem Cell Res. 2016;17:597-599 pubmed publisher
    ..8952. ..
  18. Hossini A, Megges M, Prigione A, Lichtner B, Toliat M, Wruck W, et al. Induced pluripotent stem cell-derived neuronal cells from a sporadic Alzheimer's disease donor as a model for investigating AD-associated gene regulatory networks. BMC Genomics. 2015;16:84 pubmed publisher