Research Topics
| Jürgen J HeinischSummaryCountry: Germany Publications
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Detail Information
Publications
Atomic force microscopy - looking at mechanosensors on the cell surfaceJürgen J Heinisch
Universitat Osnabruck, Fachbereich Biologie chemie, AG Genetik, Barbarastr 11, 49076 Osnabruck, Germany
J Cell Sci 125:4189-95. 2012..Although we emphasize recent studies on cell surface proteins from yeasts, the techniques described are applicable to surface proteins from virtually all organisms, from bacteria to human cells...- Single-molecule atomic force microscopy reveals clustering of the yeast plasma-membrane sensor Wsc1Jürgen J Heinisch
Fachbereich Biologie Chemie AG Genetik, Universitat Osnabruck, Osnabruck, Germany
PLoS ONE 5:e11104. 2010..cerevisiae. Our findings indicate that in yeast, like in higher eukaryotes, signalling is coupled to the localized enrichment of sensors and receptors within membrane patches... 
Measurement of the mechanical behavior of yeast membrane sensors using single-molecule atomic force microscopyJürgen J Heinisch
Fachbereich Biologie chemie, Universitat Osnabruck, Osnabruck, Germany
Nat Protoc 5:670-7. 2010..For experienced researchers in biological AFM, the entire protocol can be completed in approximately 3 weeks...- A block of endocytosis of the yeast cell wall integrity sensors Wsc1 and Wsc2 results in reduced fitness in vivoSabrina Wilk
AG Genetik, Fachbereich Biologie chemie, Universitat Osnabruck, Barbarastr 11, Osnabruck, Germany
Mol Genet Genomics 284:217-29. 2010..These data suggest that sensor distribution is not the major determinant for its specificity... - Is there anyone out there?--Single-molecule atomic force microscopy meets yeast genetics to study sensor functionsJürgen J Heinisch
Universitat Osnabruck, Fachbereich Biologie chemie, AG Genetik, Osnabruck, Germany
Integr Biol (Camb) 2:408-15. 2010.... 
Dissecting sensor functions in cell wall integrity signaling in Kluyveromyces lactisRosaura Rodicio
Universitat Osnabruck, Fachbereich Biologie chemie, AG Genetik, Barbarastr 11, D 49076 Osnabrück, Germany
Fungal Genet Biol 45:422-35. 2008..Sensor-GFP fusions localize to the plasma membrane, with a cell cycle dependent distribution of KlWsc1-GFP. Both Wsc-type sensors concentrate in discrete spots within the plasma membrane...- How do I begin? Sensing extracellular stress to maintain yeast cell wall integrityArne Jendretzki
University of Osnabruck, Faculty of Biology and Chemistry, Department of Genetics, Barbarastrasse 11, 49076 Osnabruck, Germany
Eur J Cell Biol 90:740-4. 2011..This review summarises our recent results on sensor structure and function, as well as the advances made regarding sensor mechanics... - Milk and sugar: regulation of cell wall synthesis in the milk yeast Kluyveromyces lactisKatja Backhaus
University of Osnabruck, Faculty of Biology and Chemistry, Department of Genetics, Barbarastrasse 11, 49076 Osnabruck, Germany
Eur J Cell Biol 90:745-50. 2011..lactis. We here summarize the results obtained in the past years on CWI signaling in K. lactis and use a comparative approach to the findings obtained in S. cerevisiae to highlight special adaptations to their natural environments... - Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neckArne Jendretzki
Universitat Osnabruck, Fachbereich Biologie chemie, AG Genetik, Barbarastr 11, Osnabrück 49076, Germany
Mol Genet Genomics 282:437-51. 2009..We propose that Cyk3 is part of an actomyosin ring independent cytokinesis pathway, which acts as a rescue mechanism to recruit Inn1 to the bud neck... - Functional analyses of the extra- and intracellular domains of the yeast cell wall integrity sensors Mid2 and Wsc1Andrea Straede
Universitat Osnabruck, Fachbereich Biologie chemie, AG Genetik, Barbarastr 11, D 49076 Osnabrück, Germany
FEBS Lett 581:4495-500. 2007.... - KlRHO1 and KlPKC1 are essential for cell integrity signalling in Kluyveromyces lactisRosaura Rodicio
, Fachbereich Biologie/Chemie, AG Genetik, Barbarastr. 11, , Germany
Microbiology 152:2635-49. 2006..Thus, KlPkc1 and KlRho1 have been shown to serve vital functions in K. lactis, to interact in cell integrity signalling and to traffic between the plasma membrane and the vacuole... - The effect of tea tree oil and antifungal agents on a reporter for yeast cell integrity signallingAndrea Straede
Universitat Osnabruck, Fachbereich Biologie chemie, AG Genetik, Barbarastrasse 11, D 49076 Osnabrück, Germany
Yeast 24:321-34. 2007..1% v/v, with the purified compounds acting already at half these concentrations. A mid2 deletion displayed hyper-resistance. Tea tree oil also induces the signalling pathway in a dose-dependent manner... - A tool kit for molecular genetics of Kluyveromyces lactis comprising a congenic strain series and a set of versatile vectorsJürgen J Heinisch
Universitat Osnabruck, Fachbereich Biologie chemie, AG Genetik, Osnabruck, Germany
FEMS Yeast Res 10:333-42. 2010..To increase the frequency of homologous recombination, the Klku80 deletion was also introduced into the congenic strain series. A PCR-based method for determination of mating type is provided... - Triple mammalian/yeast/bacterial shuttle vectors for single and combined Lentivirus- and Sindbis virus-mediated infections of neuronsLidia Bakota
Department of Neurobiology, University of Osnabruck, Barbarastr 11, 49076 Osnabruck, Germany
Mol Genet Genomics 287:313-24. 2012..We demonstrate the expression of genes encoding fluorescent proteins (EGFP and mCherry) both separately and in combination, using two different viral systems in mammalian cell lines, primary neurons and organotypic slices... - New yeast/E. coli/Drosophila triple shuttle vectors for efficient generation of Drosophila P element transformation constructsAchim Paululat
University of Osnabruck, Faculty of Biology, Department of Zoology and Developmental Biology, Barbarastr 11, D 49076 Osnabrück, Germany
Gene 511:300-5. 2012..The new set of triple shuttle vectors will be highly beneficial for the rapid construction of complex Drosophila transformation plasmids... - A systematic study of the cell wall composition of Kluyveromyces lactisKatja Backhaus
Universitat Osnabruck, Fachbereich Biologie chemie, AG Genetik, Osnabruck, Germany
Yeast 27:647-60. 2010..Importantly, the composition of the cell wall glycoproteome depended on carbon source and growth phase. Our results clearly illustrate the dynamic nature of the cell wall of K. lactis and provide a firm base for studying its regulation... - The frontotemporal dementia mutation R406W blocks tau's interaction with the membrane in an annexin A2-dependent mannerAnne Gauthier-Kemper
Department of Neurobiology, University of Osnabruck, Osnabruck, Germany
J Cell Biol 192:647-61. 2011..We suggest that the pathological effect of the R406W mutation is caused by impaired membrane binding, which involves a functional interaction with AnxA2 as a membrane-cytoskeleton linker... - Baker's yeast as a tool for the development of antifungal kinase inhibitors--targeting protein kinase C and the cell integrity pathwayJürgen J Heinisch
Universitat Osnabruck, Fachbereich Biologie chemie, AG Genetik, Barbarastr 11, D 49069 Osnabruck, Germany
Biochim Biophys Acta 1754:171-82. 2005..Since a variety of protein kinases take part in the signal transduction, this broadens the range of targets for potential inhibitors...