Research Topics
| Reiner F HaseloffSummaryCountry: Germany Publications
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Publications
Differential protein expression in brain capillary endothelial cells induced by hypoxia and posthypoxic reoxygenationReiner F Haseloff
Institute of Molecular Pharmacology, Berlin, Germany
Proteomics 6:1803-9. 2006..g., calreticulin), and cytoskeletal (e.g., vimentin) proteins. The results indicate that BCEC respond to hypoxia/reoxygenation by adaptive up-regulation of proteins involved in the glycolysis, protein synthesis, and stress response...- Cytotoxicity of spin trapping compoundsR F Haseloff
Research Institute of Molecular Pharmacology, Berlin, Germany
FEBS Lett 418:73-5. 1997..The results of this study indicate that most spin traps can be used in cell cultures at customary (i.e. millimolar) concentrations; caution is recommended when nitroso spin traps are applied to cells... - In search of the astrocytic factor(s) modulating blood-brain barrier functions in brain capillary endothelial cells in vitroR F Haseloff
Forschungsinstitut fur Molekulare Pharmakologie, Robert Rossle Str 10, D 13125 Berlin, Germany
Cell Mol Neurobiol 25:25-39. 2005..4) In conclusion, it is expected that the present and future research focused on molecular mechanisms and signaling pathways will produce new and exciting insights into the complex network of BBB regulation: the cornerstone is laid... - A strategy for enrichment of claudins based on their affinity to Clostridium perfringens enterotoxinDörte Lohrberg
Leibniz Institut fur Molekulare Pharmakologie FMP, Berlin, Germany
BMC Mol Biol 10:61. 2009..The interaction of several claudins with Clostridium perfringens enterotoxin (CPE) has been exploited for an affinity-based enrichment of CPE-binding claudins from lysates of normal rat cholangiocytes... - Superoxide-mediated reduction of the nitroxide group can prevent detection of nitric oxide by nitronyl nitroxidesR F Haseloff
Institute of Molecular Pharmacology, Berlin, Germany
Free Radic Res 26:7-17. 1997..The results indicate that in biological systems (in addition to other reducing agents) the presence of superoxide can prevent the detection of nitric oxide using nitronyl nitroxides... - Nitronyl nitroxides, a novel group of protective agents against oxidative stress in endothelial cells forming the blood-brain barrierI E Blasig
Forschungsinstitut fur Molekulare Pharmakologie, Delbrück Zentrum für Molekulare Medizin, Robert Rossle Str 10, 13125 Berlin, Germany
Neuropharmacology 43:1006-14. 2002..NN represent a new group of antioxdatively acting cytoprotectiva improving the survival and function of the endothelium against oxidative stress... - *NO and oxyradical metabolism in new cell lines of rat brain capillary endothelial cells forming the blood-brain barrierI E Blasig
Forschungsinstitut fur Molekulare Pharmakologie, Robert Rossle Str 10, 10315, Berlin, Germany
Microvasc Res 62:114-27. 2001..These cells can be subcultivated in large amounts and, hence, they are suitable to study the role of radical metabolism in the BBB and in the cerebral microvasculature... - Proteomics of brain endothelium. Separation of proteins by two-dimensional gel electrophoresis and identification by mass spectrometryReiner F Haseloff
Institute of Molecular Pharmacology, Berlin, Germany
Methods Mol Med 89:465-77. 2003 - Structure and function of claudinsGerd Krause
Leibniz Institut fur Molekulare Pharmakologie, Robert Rossle Str 10, 13125 Berlin, Germany
Biochim Biophys Acta 1778:631-45. 2008..The concepts evolved from these findings and first tentative molecular models for homophilic interactions may explain the different functional contribution of the two extracellular loops at tight junctions... - Tight junctions and tissue barriersIngolf E Blasig
Leibniz Institut fur Molekulare Pharmakologie, Berlin, Germany
Antioxid Redox Signal 15:1163-6. 2011..However, our knowledge of the molecular basis of the specific mechanisms responsible for functional disturbances remains limited and needs further investigations. Antioxid. Redox Signal. 15, 1163-1166... - Synthesis and spin trapping applications of 2,2-dimethyl-d6-4-methyl-2H-imidazole-1-oxide-1-15NR F Haseloff
Institute of Molecular Pharmacology, Berlin, Germany
Free Radic Res 26:159-68. 1997..The pKa was found to be 5.8 +/- 0.3. In comparison to other nitrones, application of this spin trap provides more detailed information on the structure of the species trapped, especially for carbon-centered radicals... - Evaluation of the titanium dioxide approach for MS analysis of phosphopeptidesClementine Klemm
, , 13125 Berlin, Germany
J Mass Spectrom 41:1623-32. 2006..Drastically reduced recovery was observed for phosphopeptides with multiple basic amino acids. Nonspecific binding of nonphosphorylated peptides and sample loss of phosphopeptides must also be taken into account... - Protein markers of ischemic insult in brain endothelial cells identified using 2D gel electrophoresis and ICAT-based quantitative proteomicsArsalan S Haqqani
Cerebrovascular Research and Genomics and Proteomics Groups, Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, Canada K1A 0R6
J Proteome Res 6:226-39. 2007..The comparative analyses of protein changes between the in vitro and in vivo models demonstrated a significant correlative relationship, emphasizing the 'translational' value of in vitro endothelial models in neurovascular research... - Protective effects of peroxiredoxin-1 at the injured blood-brain barrierGerty Schreibelt
Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands
Free Radic Biol Med 45:256-64. 2008..On the other hand, it may function as an endogenous defense mechanism to inhibit leukocyte infiltration and counteract ROS-induced cellular injury...