Affiliation: Hannover Medical School
Saran S, Tran D, Klebba Färber S, Morán Losada P, Wiehlmann L, Koch A, et al
. THOC5, a member of the mRNA export complex, contributes to processing of a subset of wingless/integrated (Wnt) target mRNAs and integrity of the gut epithelial barrier. BMC Cell Biol. 2013;14:51 pubmed publisher
..These data imply that THOC5 may be a useful tool for studying intestinal stem cells, for modifying the differentiation processes and for cancer therapy. ..
Tran D, Saran S, Williamson A, Pierce A, Dittrich Breiholz O, Wiehlmann L, et al
. THOC5 controls 3'end-processing of immediate early genes via interaction with polyadenylation specific factor 100 (CPSF100). Nucleic Acids Res. 2014;42:12249-60 pubmed publisher
..THOC5 is required for recruitment of CPSF100 to 3'UTR of THOC5 target genes. These data suggest the presence of a novel mechanism for the control of IEG response by THOC5 via 3'end-processing. ..
Koch A, Saran S, Tran D, Klebba FÃ¤rber S, Thiesler H, Sewald K, et al
. Murine precision-cut liver slices (PCLS): a new tool for studying tumor microenvironments and cell signaling ex vivo. Cell Commun Signal. 2014;12:73 pubmed publisher
..This method may also prove useful for identification of genes in liver-resident cells that support cancer invasion by using PCLS from transgenic liver. ..
Tran D, Saran S, Koch A, Tamura T. mRNA export protein THOC5 as a tool for identification of target genes for cancer therapy. Cancer Lett. 2016;373:222-6 pubmed publisher
..Finally we describe our recent data on selection of targeting candidates from THOC5 dependent genes for application in cancer therapy. ..
Tran D, Koch A, Saran S, Armbrecht M, Ewald F, Koch M, et al
. Extracellular-signal regulated kinase (Erk1/2), mitogen-activated protein kinase-activated protein kinase 2 (MK2) and tristetraprolin (TTP) comprehensively regulate injury-induced immediate early gene (IEG) response in in vitro liver organ culture. Cell Signal. 2016;28:438-447 pubmed publisher
..These data also suggest that in vitro liver culture may be a useful tool for measuring the proliferation potential of hepatocytes in individual liver. ..
Tran D, Koch A, Allister A, Saran S, Ewald F, Koch M, et al
. Treatment with MAPKAP2 (MK2) inhibitor and DNA methylation inhibitor, 5-aza dC, synergistically triggers apoptosis in hepatocellular carcinoma (HCC) via tristetraprolin (TTP). Cell Signal. 2016;28:1872-1880 pubmed publisher
..Notably, under the same conditions human hepatocyte cells (THLE-2) did not undergo apoptosis. These data also suggest that MK2 inhibitor with 5-aza dC or interferon may be a useful tool for therapy against HCC. ..
Dutta P, Koch A, Breyer B, Schneider H, Dittrich Breiholz O, Kracht M, et al
. Identification of novel target genes of nerve growth factor (NGF) in human mastocytoma cell line (HMC-1 (V560G c-Kit)) by transcriptome analysis. BMC Genomics. 2011;12:196 pubmed publisher
..However, the exact role of NGF in hematopoietic system is unclear. It is also not clear whether NGF mediated signals in hematopoietic cells are identical to those in neuronal cells...
Mancini A, Niemann Seyde S, Pankow R, El Bounkari O, Klebba Färber S, Koch A, et al
. THOC5/FMIP, an mRNA export TREX complex protein, is essential for hematopoietic primitive cell survival in vivo. BMC Biol. 2010;8:1 pubmed publisher
..Furthermore, mechanistically depletion of THOC5/Fms interacting protein causes the down-regulation of its direct interacting partner, THOC1 which may contribute to altered THO complex function and cell death. ..