Research Topics
Genomes and Genes
| Ursula SeidlerSummaryAffiliation: Hannover Medical School Country: Germany Publications
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Detail Information
Publications
Essential role of the electroneutral Na+-HCO3- cotransporter NBCn1 in murine duodenal acid-base balance and colonic mucus layer build-up in vivoAnurag Kumar Singh
U Seidler Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Carl Neuberg Strase 1, D 30625 Germany
J Physiol 591:2189-204. 2013....
Sodium and chloride absorptive defects in the small intestine in Slc26a6 null miceUrsula Seidler
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Pflugers Arch 455:757-66. 2008..The data strongly suggests that Slc26a6 acts in concert with NHE3 in electroneutral salt absorption in the small intestine. Slc26a6 also serves to absorb Cl(-) during glucose-driven salt absorption...- The role of the NHERF family of PDZ scaffolding proteins in the regulation of salt and water transportUrsula Seidler
Department of Gastroenterology, Hannover Medical School, Hannover, Germany
Ann N Y Acad Sci 1165:249-60. 2009..Recently, NHERF1, 2, and 3 knockout mice have become available, and this review summarizes the reports on electrolyte and fluid transport regulation in the native intestine of these mice... - Molecular mechanisms of disturbed electrolyte transport in intestinal inflammationUrsula Seidler
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany
Ann N Y Acad Sci 1072:262-75. 2006.... - Knockout mouse models for intestinal electrolyte transporters and regulatory PDZ adaptors: new insights into cystic fibrosis, secretory diarrhoea and fructose-induced hypertensionUrsula Seidler
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Germany
Exp Physiol 94:175-9. 2009.... - Recent advances in the molecular and functional characterization of acid/base and electrolyte transporters in the basolateral membranes of gastric and duodenal epithelial cellsU Seidler
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Germany
Acta Physiol (Oxf) 201:3-20. 2011..The purpose of this review is to summarize current knowledge on the molecular identity and cellular function of basolateral ion transporters in the gastric and duodenal epithelium... 
The switch of intestinal Slc26 exchangers from anion absorptive to HCOFormula secretory mode is dependent on CFTR anion channel functionAnurag Kumar Singh
Dept of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Germany
Am J Physiol Cell Physiol 298:C1057-65. 2010..This suggests that Slc26a6 and Slc26a3 need proton recycling via NHE3 to operate in the Cl(-) absorptive mode and Cl(-) exit via CFTR to operate in the HCO(3)(-) secretory mode...- Loss of PDZ-adaptor protein NHERF2 affects membrane localization and cGMP- and [Ca2+]- but not cAMP-dependent regulation of Na+/H+ exchanger 3 in murine intestineMingmin Chen
Department of Gastroenterology, Hannover Medical School, Hannover 30625, Germany
J Physiol 588:5049-63. 2010.... - NHE3 inhibition by cAMP and Ca2+ is abolished in PDZ-domain protein PDZK1-deficient murine enterocytesAyhan Cinar
Hannover Medical School, Department of Gastroenterology and Hepatology, Carl Neuberg Str 1, 30625 Hannover, Germany
J Physiol 581:1235-46. 2007..It leaves NHE3 inhibition by hyperosmolarity intact, suggesting an important and selective role for PDZK1 in the agonist-mediated regulation of intestinal NHE3 activity... - Gene ablation for PEPT1 in mice abolishes the effects of dipeptides on small intestinal fluid absorption, short-circuit current, and intracellular pHMingmin Chen
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Carl Neuberg Strasse 1, D 30625 Hannover, Germany
Am J Physiol Gastrointest Liver Physiol 299:G265-74. 2010.... - Differential roles of NHERF1, NHERF2, and PDZK1 in regulating CFTR-mediated intestinal anion secretion in miceAnurag Kumar Singh
Department of Gastroenterology, Hepatology, and Endocrinology, Carl Neuberg Strasse 1, Hannover Medical School, Hannover, Germany
J Clin Invest 119:540-50. 2009..These data suggest that the NHERF proteins differentially modulate duodenal HCO3- secretion: while NHERF1 is an obligatory linker for beta2-AR stimulation of CFTR, NHERF2 confers inhibitory signals by coupling the LPA receptor to CFTR... - Rescue of epithelial HCO3- secretion in murine intestine by apical membrane expression of the cystic fibrosis transmembrane conductance regulator mutant F508delFang Xiao
Department of Gastoenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
J Physiol 590:5317-34. 2012..This suggests that therapeutic strategies which deliver F508del to the apical membrane have the potential to significantly enhance epithelial HCO(3)(-) secretion... - Down regulation of small intestinal ion transport in PDZK1- (CAP70/NHERF3) deficient miceJutta Hillesheim
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Carl Neuberg Strasse 1, 30625 Hannover, Germany
Pflugers Arch 454:575-86. 2007..Lack of the PDZ-adapter protein PDZK1 in murine small intestine causes a mild reduction in maximal CFTR activation, but a severe defect in electroneutral Na(+) absorption... - Kir4.1 channel expression is essential for parietal cell control of acid secretionPenghong Song
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, D 30625 Hannover, Germany
J Biol Chem 286:14120-8. 2011..Our results demonstrate that Kir4.1 channels are involved in the control of acid secretion and suggest that they may also affect secretory membrane recycling... - Localization, trafficking, and significance for acid secretion of parietal cell Kir4.1 and KCNQ1 K+ channelsMarc André Kaufhold
Department of Gastroenterology, Hannover Medical School, Hannover, Germany
Gastroenterology 134:1058-69. 2008..This study addresses the subcellular localization, trafficking, and potential functional significance of KCNQ1 and Kir4.1 channels during stimulated acid secretion... - The electroneutral Na⁺:HCO₃⁻ cotransporter NBCn1 is a major pHi regulator in murine duodenumMingmin Chen
Department of Gastroenterology, Hannover Medical School, Carl Neuberg Strase 1, Hannover, D 30625, Germany
J Physiol 590:3317-33. 2012..The electroneutral Na+:HCO3 − cotransporter NBCn1 (slc4a7) is a major duodenal HCO3 − importer that supplies HCO3 − during basal and FSK-stimulated HCO3 − secretion... - Duodenal acidity "sensing" but not epithelial HCO3- supply is critically dependent on carbonic anhydrase II expressionMarkus Sjöblom
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover 30625, Germany
Proc Natl Acad Sci U S A 106:13094-9. 2009..The results suggest that CA II is important for duodenocyte acidification by low luminal pH and for eliciting the acid-mediated HCO(3)(-) secretory response, but is not important in the generation of the secreted HCO(3)(-) ions... - cAMP-dependent and cholinergic regulation of the electrogenic intestinal/pancreatic Na+/HCO3- cotransporter pNBC1 in human embryonic kidney (HEK293) cellsOliver Bachmann
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany
BMC Cell Biol 9:70. 2008..After having shown activation of NBC by carbachol and forskolin in murine colon, we now investigated these pathways in HEK293 cells transiently expressing a GFP-tagged pNBC1 construct... - Involvement of the anion exchanger SLC26A6 in prostaglandin E2- but not forskolin-stimulated duodenal HCO3- secretionBiguang Tuo
Department of Gastroenterology and Hepatology, Hannover Medical School, Germany
Gastroenterology 130:349-58. 2006..The present study was designed to examine the role of SLC26A6 in prostaglandin E(2) (PGE(2))-, forskolin-, and carbachol-induced duodenal HCO(3)(-) secretion... - Preserved Na(+)/H(+) exchanger isoform 3 expression and localization, but decreased NHE3 function indicate regulatory sodium transport defect in ulcerative colitisSunil Yeruva
Department of Gastroenterology, Hannover Medical School, Hannover, Germany
Inflamm Bowel Dis 16:1149-61. 2010..We therefore studied the expression, brush border membrane (BBM) localization, and transport capacity of the major intestinal Na(+) absorptive protein, the Na(+)/H(+) exchanger isoform 3 (NHE3) in biopsies from UC patients... - Loss of downregulated in adenoma (DRA) impairs mucosal HCO3(-) secretion in murine ileocolonic inflammationFang Xiao
Department of Gastroenterology, Hannover Medical School, Germany
Inflamm Bowel Dis 18:101-11. 2012..Disturbances in J(HCO3)(-) may occur due to inflammation-induced changes in the crypt and villous architecture, or due to the effect of proinflammatory cytokines on epithelial ion transporters... - Downregulation of the NHE3-binding PDZ-adaptor protein PDZK1 expression during cytokine-induced inflammation in interleukin-10-deficient miceHenrike Lenzen
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
PLoS ONE 7:e40657. 2012.... - KCNQ1 is the luminal K+ recycling channel during stimulation of gastric acid secretionPenghong Song
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Carl Neuberg Strasse 1, D 30625, Germany
J Physiol 587:3955-65. 2009..The study demonstrates that the KCNQ1 channel provides K(+) to the extracellular K(+) binding site of the H(+)/K(+)-ATPase during acid secretion, and no other gastric K(+) channel can substitute for this function... - Fructose-induced hypertension: essential role of chloride and fructose absorbing transporters PAT1 and Glut5Anurag Kumar Singh
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Kidney Int 74:438-47. 2008..We suggest that fructose-induced hypertension is likely caused by increased salt absorption by the intestine and kidney and the transporters Slc26a6 and Slc2a5 are essential in this process... - Neuronal cGMP kinase I is essential for stimulation of duodenal bicarbonate secretion by luminal acidAnurag Kumar Singh
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany
FASEB J 26:1745-54. 2012..vagus elicited a similar HCO(3)(-) secretory response in cGKI(-/-), ncGKI(-/-) and wild-type mice. These findings show that protection of the duodenum from acid injury requires neuronal cGKI... - Phosphatidylinositol 3-kinase is involved in prostaglandin E2-mediated murine duodenal bicarbonate secretionBi guang Tuo
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Carl Neuberg Strasse 1, 30625 Hannover, Germany
Am J Physiol Gastrointest Liver Physiol 293:G279-87. 2007..These results demonstrated that PGE(2) activates PI3K in duodenal mucosa and stimulates duodenal HCO(3)(-) secretion via cAMP-, Ca(2+)-, and PI3K-dependent signaling pathways... - News from the end of the gut--how the highly segmental pattern of colonic HCO₃⁻ transport relates to absorptive function and mucosal integrityOliver Bachmann
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany
Biol Pharm Bull 34:794-802. 2011.... - Increased epithelial permeability is the primary cause for bicarbonate loss in inflamed murine colonMarina Jurić
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
Inflamm Bowel Dis 19:904-11. 2013..However, candidate pathways like CFTR or DRA are inhibited in the inflamed gut. This study addressed the question whether and how inflammation-associated increased intestinal permeability may result in epithelial HCO(3)(-) loss... - Cytoprotective effects of acidosis via heat shock protein HSP27 against the anticancer drug doxorubicinAnurag Kumar Singh
Department of Gastroenterolgy, Hepatology and Endocrinology, Hannover Medical School, Germany
Cell Mol Life Sci 68:1041-51. 2011..In conclusion, acidic pH causes less accumulation of cytotoxic drug in the nucleus of adeno gastric carcinoma (AGS) cells and HSP27-dependent decrease in FasR-mediated gastric epithelial tumour cell apoptosis... - Mechanisms of secretion-associated shrinkage and volume recovery in cultured rabbit parietal cellsOliver Bachmann
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany
Am J Physiol Gastrointest Liver Physiol 292:G711-7. 2007..K(+), Cl(-), and water secretion into the secretory canaliculi is thus one likely mechanism of stimulation-associated cell shrinkage in cultured parietal cells. The observed RVI is predominantly mediated by NHE1... - Protein kinase C-alpha attenuates cholinergically stimulated gastric acid secretion of rabbit parietal cellsMichael Fährmann
Institut für Zoophysiologie der Westfälischen Wilhelms Universität Münster, Hindenburgplatz 55, D 48143 Munster, Germany
Br J Pharmacol 139:545-54. 2003..We assume that in cholinergically stimulated parietal cells PKC-alpha transinhibits CaMKII activity, resulting in an attenuation of acid secretion... - Identification of a basolateral Cl-/HCO3- exchanger specific to gastric parietal cellsSnezana Petrovic
Department of Medicine, University of Cincinnati, Cincinnati 45267, USA
Am J Physiol Gastrointest Liver Physiol 284:G1093-103. 2003..On the basis of its unique expression pattern and function, we propose that SLC26A7 is a basolateral Cl(-)/HCO(3)(-) exchanger in gastric parietal cells and plays a major role in gastric acid secretion... - Colocalization of the apical Cl-/HCO3- exchanger PAT1 and gastric H-K-ATPase in stomach parietal cellsSnezana Petrovic
Department of Medicine, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267 0585, USA
Am J Physiol Gastrointest Liver Physiol 283:G1207-16. 2002.... - The down regulated in adenoma (dra) gene product binds to the second PDZ domain of the NHE3 kinase A regulatory protein (E3KARP), potentially linking intestinal Cl-/HCO3- exchange to Na+/H+ exchangeGeorg Lamprecht
1st Medical Department and Medical and Natural Sciences Research Center, University of Tuebingen, Otfried Mueller Strasse 10, 72076 Tuebingen, Germany
Biochemistry 41:12336-42. 2002..In such a model, the first PDZ domain would remain available for instance for signal transduction proteins... - Renal and intestinal transport defects in Slc26a6-null miceZhaohui Wang
Department of Internal Medicine, Division of Nephrology and Hypertension, Univ of Cincinnati, 231 Albert Sabin Way, MSB 259G, Cincinnati OH 45267 0585, USA
Am J Physiol Cell Physiol 288:C957-65. 2005..We conclude that Slc26a6 mediates oxalate-stimulated NaCl absorption, contributes to apical membrane Cl-/base exchange in the kidney proximal tubule, and also plays an important role in HCO3- secretion in the duodenum... - The CFTR associated protein CAP70 interacts with the apical Cl-/HCO3- exchanger DRA in rabbit small intestinal mucosaHeidi Rossmann
Department of Clinical Chemistry and Laboratory Medicine, University of Mainz, Mainz, Germany
Biochemistry 44:4477-87. 2005..Its restricted expression shows that it cannot be involved in NaCl absorption in the proximal colon. We suggest that CAP70 mediates regulatory functions specific to the small intestine... - Different actions of protein kinase C isoforms alpha and epsilon on gastric acid secretionMichael Fährmann
Institut für Zoophysiologie der Westfälischen Wilhelms Universität Münster, Hindenburgplatz 55, D 48143 Munster, Germany
Br J Pharmacol 136:938-46. 2002..In contrast, PKC-alpha activation attenuates acid secretion accompanied by a down-regulation of CaMKII activity... - Down-regulated in adenoma mediates apical Cl-/HCO3- exchange in rabbit, rat, and human duodenumPetra Jacob
Department of Medicine, University of Tubingen, Tubingen, Germany
Gastroenterology 122:709-24. 2002..The recently discovered dra (down-regulated in adenoma) gene encodes a transport protein (DRA) for SO4(2-), Cl-, and HCO3-. The aim of this study was to investigate whether DRA may be the duodenal apical Cl-/HCO3- exchanger... - Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integritySya N Ukena
Department of Mucosal Immunity, Helmholtz Centre for Infection Research, Braunschweig, Germany
PLoS ONE 2:e1308. 2007..Disruption of this border alters paracellular permeability and is a key mechanism for the development of enteric infections and inflammatory bowel diseases (IBDs)... - CaMKII is activated and translocated to the secretory apical membrane during cholinergically conveyed gastric acid secretionMichael Fährmann
Institut für Zoophysiologie der Westfälischen Wilhelms Universität Münster, Hindenburgplatz 55, D 48143 Munster, Germany
Cell Signal 14:161-8. 2002..These findings contribute to the model implicating an involvement for CaMKII in the intracellular dynamics of the acid secretion... - Isoforms of SLC26A6 mediate anion transport and have functional PDZ interaction domainsHannes Lohi
Department of Medical Genetics, Biomedicum Helsinki, University of Helsinki, Finland
Am J Physiol Cell Physiol 284:C769-79. 2003..Our results support the general concept of microdomain organization for ion transport and suggest a mechanism for cystic fibrosis transmembrane regulator (CFTR)-mediated SLC26A6 upregulation in pancreatic duct cells... - Cystic fibrosis transmembrane conductance regulator activation is reduced in the small intestine of Na+/H+ exchanger 3 regulatory factor 1 (NHERF-1)- but Not NHERF-2-deficient miceNellie Broere
Department of Biochemistry, Erasmus University Medical Center, 3015 GE, Rotterdam, The Netherlands
J Biol Chem 282:37575-84. 2007.... - Identification of an apical Cl-/HCO3- exchanger in gastric surface mucous and duodenal villus cellsJie Xu
Department of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
Am J Physiol Gastrointest Liver Physiol 285:G1225-34. 2003..These data show that AE4 is an apical Cl-/HCO3- exchanger in gastric mucous cells and duodenal villus cells. On the basis of its function and location, we propose that AE4 may play an important role in mucosal protection... - Controversies in the role of SLC26 anion exchangers in pancreatic ductal bicarbonate secretionPeter Hegyi
Pancreas 37:232-4. 2008 - Role of Na-K-2Cl cotransporter-1 in gastric secretion of nonacidic fluid and pepsinogenNichole McDaniel
Div of Biomedical Sciences, 2226 Webber Hall, Univ of California, Riverside, CA 92521, USA
Am J Physiol Gastrointest Liver Physiol 289:G550-60. 2005..The observed dependence of pepsinogen secretion on NKCC supports the concept that a nonacidic secretory stream elaborated from parietal cells facilitates flushing of the proenzyme from the gastric gland lumen... - Intravenous iron sucrose versus oral iron supplementation for the treatment of iron deficiency anemia in patients with inflammatory bowel disease--a randomized, controlled, open-label, multicenter studyOliver Schroder
First Department of Internal Medicine, Division of Gastroenterology, ZAFES, Johann Wolfgang Goethe University, Frankfurt, Germany
Am J Gastroenterol 100:2503-9. 2005..We therefore evaluated the efficacy and safety of intravenous iron sucrose as compared with oral iron sulfate for the treatment of iron deficiency anemia (IDA) in patients with IBD... - Tissue-specific regulation of sodium/proton exchanger isoform 3 activity in Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) null mice. cAMP inhibition is differentially dependent on NHERF1 and exchange protein directly activated by cAMP in ileum versus Rakhilya Murtazina
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 282:25141-51. 2007.... - Gp130 signaling promotes development of acute experimental colitis by facilitating early neutrophil/macrophage recruitment and activationLeif E Sander
Department of Medicine III, RWTH University Hospital Aachen, Aachen, Germany
J Immunol 181:3586-94. 2008..We provide evidence that IL-6 cytokines acting via gp130 are required in the acute stages of intestinal inflammation by modulating the dynamics of innate immune cell recruitment and activation...