- Human selenoproteins at a glanceS Gromer
Biochemiezentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 504, 69120, Heidelberg, Germany
Cell Mol Life Sci 62:2414-37. 2005..In this review we provide a brief but up-to-date overview of what is currently known about these 25 acknowledged human selenoproteins and their synthesis...
- Active sites of thioredoxin reductases: why selenoproteins?Stephan Gromer
Biochemie Zentrum Heidelberg, Heidelberg University, Im Neuenheimer Feld 504, D 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 100:12618-23. 2003..Our data suggest that the selective advantage of selenoenzymes is a broader range of substrates and a broader range of microenvironmental conditions in which enzyme activity is possible...
- The thioredoxin system--from science to clinicStephan Gromer
Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 504, D 69120 Heidelberg, Germany
Med Res Rev 24:40-89. 2004..In this review we will present and evaluate the preclinical and clinical results available today. Current trends in drug development are emphasized...
- Interactions of methylene blue with human disulfide reductases and their orthologues from Plasmodium falciparumKathrin Buchholz
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 504, D 69120 Heidelberg, Germany
Antimicrob Agents Chemother 52:183-91. 2008..This explains the terms subversive substrate or turncoat inhibitor for MB. The results are discussed in cell-pathological and clinical contexts...
- Mutational studies confirm the catalytic triad in the human selenoenzyme thioredoxin reductase predicted by molecular modelingStephan Gromer
Heidelberg University Biochemistry Center, Im Neuenheimer Feld 504, 69120 Heidelberg, Germany
Chembiochem 7:1649-52. 2006
- Cytotoxic interactions of methylene blue with trypanosomatid-specific disulfide reductases and their dithiol productsKathrin Buchholz
Biochemie Zentrum der Universitat Heidelberg, INF 504, D 69120 Heidelberg, Germany
Mol Biochem Parasitol 160:65-9. 2008..Since MB is an affordable, available, and accessible drug it might be tested--alone or in drug combinations--against trypanosomatid-caused diseases of animal and man...
- The conserved histidine 106 of large thioredoxin reductases is likely to have a structural role but not a base catalyst functionJudit Jacob
Biochemiezentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 504, D 69120 Heidelberg, Germany
FEBS Lett 579:745-8. 2005..Interestingly, the phenylalanine-mutants, designed as negative controls were the most active mutants which suggests rather a structural role of His106...
- Human placenta thioredoxin reductase: preparation and inhibitor studiesStephan Gromer
Biochemistry Center, Heidelberg University, D 69120 Heidelberg, Germany
Methods Enzymol 347:382-94. 2002
- Thioredoxin reductase from the malaria mosquito Anopheles gambiaeHolger Bauer
Biochemie Zentrum, Universitat Heidelberg, Heidelberg, Germany
Eur J Biochem 270:4272-81. 2003..These differences offer an interesting approach to the design of species-specific inhibitors. Notably, A. gambiae thioredoxin reductase-1 is not a selenoenzyme but instead contains a highly unusual redox-active Cys-Cys sequence...
- Methylseleninate is a substrate rather than an inhibitor of mammalian thioredoxin reductase. Implications for the antitumor effects of seleniumStephan Gromer
Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
J Biol Chem 277:9701-6. 2002..This suggests that the catalytic selenocysteine residue of mammalian thioredoxin reductase is essential for methylseleninate reduction...
- Synthesis of 5-nitro-2-furancarbohydrazides and their cis-diamminedichloroplatinum complexes as bitopic and irreversible human thioredoxin reductase inhibitorsRégis Millet
UMR 8525 CNRS Université Lille II Institut Pasteur de Lille, Institut de Biologie de Lille, 1 rue du Professor Calmette, BP447 59021 Lille Cedex, France
J Med Chem 48:7024-39. 2005..Studies with mutant enzymes clearly demonstrate the penultimate selenocysteine residue as the prime target of the synthesized cis-diamminedichloroplatinum complexes...
- Selenium metabolism in Trypanosoma: characterization of selenoproteomes and identification of a Kinetoplastida-specific selenoproteinAlexey V Lobanov
Department of Biochemistry, University of Nebraska, Lincoln, NE 68588, USA
Nucleic Acids Res 34:4012-24. 2006..Overall, these data establish that Trypanosoma, Leishmania and likely other Kinetoplastida utilize and depend on the trace element selenium, and this dependence is due to occurrence of selenium in at least three selenoproteins...